MD04G1066500.v1.1

Phosphatidylinositol glycan anchor biosynthesis class U

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009567
Physical Location & Seq
Reverse (-)
8964185 .. 8970098
5914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1066500.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGGGCGGCGGCATCAGTGCTATTCAGGCTCGTTCTCATCTACTACCCAAAAATCTCAACCTTTCCTCTCGCCCCGAAGTCTCCACTCCTCTCACCAGCCTCCGCCGCCTTGCTGAGGGTTACTGGTTGAAGCAATCATCAATGTCTCCCTATGCAGGATCTATGTACCATGGATCTCCTTTGTTGCTCTCACTTCTCGGGCCACTCACAGTCAAGCGATTTGAAGGGCAACCTAATCATCTTCTCTGCAGTTTGGTTTATGTGGTTGCAGATGTATTGAATGCAATGCTTATTCGTGCTACTGGTCAGACTCTTCAGGTTGCATACCGGCAGAGTTTGAAGTCACTAGATCTTGATACGACATTAGAAAGCATTGAGGTGCTCCCTTCTGGGGATATTGCTGCTCTTGTGTATCTATGGAATCCTTTCACAATAGTTGCATGTGTGGGTTTGTCAACATCTCCAATTGAGAATTTTGCGATCATACTGGCCTTGTATGGAGCATGTAAAAGACTGGTTCCCTTGGCTGCTTTTGGGTGGGTTATAGCAACACATCTGTCTCTTTATCCTATGATTCTCCTAATTCCAATGATTCTTTTATTAGGATATGGTCCAGATACTCCTAGGAAACTGTTGCTACTGAGGAGATATGGTGAAGTTGAAGATAACTCCTCAAGTGATAGTTGTCAACAACAGGAGAAAGTTATTAATCAATCAGAGCTACCCCCTGTTTTCTCTTGGAGACCTGTCGGACTTTTCTTATTGTGGGCTATTATGTGGTCAGCCTACGTGTTATTTCTCTGTGGCGTATCTGCTAAACAGTATGGCGATCTCTGGGAGATGTTTGAAAGAATGTATGGTTTCATTCTCACTGTGCAAGATTTGTCTCCAAATATAGGTGTCTTATGGTATTTCTTTGCAGAGGTCTTCGATTTTTTCAGAAATTTCTTCCTGATAGTTTTCCAAATGAATATTCTCTTTTTGATATTTCCATTGGCCATACGACTGAAGCATCGTCCGTGCTTTCTTGCTTTTGTCTACATCACCATCTCCTCAATGCTTAAGTCTTATCCTTCTGTTGGGGATTCAGCTCTGTACTTTGGTTTGTTGGGCTGGTTTGTTAATGAACTGTCAGAAATGCGATTCTCTTTCTGCCTCTTCAGTGTTTATGTTGGCATTTCCCTCCTTAGCCCTGTGATGCACAACCTATGGATATGGAGGGGCACTGGCAATGCAAATTTCTACTTTGCTACTGCAATTACTTATGCTTGTTTGCAGATTGTTTTGGTGATTGAGAGTGTAAGTGCAATGCTCAATCATGACAGGAAGCTAAGAAAGTTGTTGAACACAAAGATTCATGTTGACAAATCTTAA

Protein Analysis

458

Amino Acids

51.56

Weight (kDa)

8.66

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-U PF06728 18 - 422 1.1e-110 GPI transamidase subunit PIG-U
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12730 AT1G12730 AT1G63110 AT1G63110 AT1G63110
fragaria_vesca FvH4_5g01570 FvH4_5g01570
malus_domestica MD04G1066500.v1.1 MD06G1073700.v1.1
prunus_persica Prupe.5G091900_v2.0.a1
pyrus_communis pycom04g05980 pycom06g07290
rosa_chinensis RchiOBHm_Chr7g0199111
rosa_laevigata RLG00000003814
rosa_multiflora Rmu_sc0006266.1_g000049
rosa_roxburghii Rroxscaffold_3G00257070
rosa_rugosa Rorug07G0052700 Rorug07G0052800
rosa_samantha Rh7BG179700 Rh7DG179200
rosa_wichuraiana Rw7G015460 Rw7G015490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1038
AciI CCGC 4 cut(s) 6, 9, 103, 106
AclWI GGATC 2 cut(s) 166, 181
AcoI YGGCCR 1 cut(s) 996
AcsI RAATTY 3 cut(s) 472, 943, 1237
AcuI CTGAAG 3 cut(s) 299, 1028, 1144
AfaI GTAC 2 cut(s) 167, 1097
AfiI CCNNNNNNNGG 5 cut(s) 115, 155, 391, 896, 1079
AflII CTTAAG 1 cut(s) 1061
AflIII ACRYGT 1 cut(s) 789
AgsI TTSAA 7 cut(s) 130, 224, 280, 340, 662, 848, 1345
AjuI GAANNNNNNNTTGG 2 cut(s) 957, 989
AluBI AGCT 3 cut(s) 721, 1091, 1330
AluI AGCT 3 cut(s) 721, 1091, 1330
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 5 cut(s) 85, 150, 564, 736, 891
AlwI GGATC 2 cut(s) 166, 181
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 3 cut(s) 200, 489, 996
ApeKI GCWGC 2 cut(s) 401, 527
ApoI RAATTY 3 cut(s) 472, 943, 1237
AseI ATTAAT 1 cut(s) 708
Asp700I GAANNNNTTC 1 cut(s) 425
AspA2I CCTAGG 1 cut(s) 623
AspS9I GGNCC 2 cut(s) 200, 611
AsuHPI GGTGA 4 cut(s) 85, 665, 1036, 1300
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 1 cut(s) 611
AvrII CCTAGG 1 cut(s) 623
BaeGI GKGCMC 1 cut(s) 1226
BalI TGGCCA 1 cut(s) 998
BbsI GAAGAC 1 cut(s) 919
Bbv12I GWGCWC 1 cut(s) 384
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 388, 514
BccI CCATC 1 cut(s) 1055
BcoDI GTCTC 5 cut(s) 85, 150, 564, 736, 891
BfaI CTAG 2 cut(s) 347, 624
BfmI CTRYAG 1 cut(s) 247
BfrI CTTAAG 1 cut(s) 1061
BglII AGATCT 1 cut(s) 349
BisI GCNGC 5 cut(s) 7, 10, 106, 402, 528
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 5 cut(s) 8, 11, 107, 403, 529
Bme18I GGWCC 1 cut(s) 611
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 200, 611
BmiI GGNNCC 1 cut(s) 519
BmsI GCATC 3 cut(s) 21, 1021, 1188
BpiI GAAGAC 1 cut(s) 919
Bpu10I CCTNAGC 2 cut(s) 114, 1187
BpuEI CTTGAG 1 cut(s) 658
BsaAI YACGTR 1 cut(s) 790
BsaI GGTCTC 1 cut(s) 736
BsaJI CCNNGG 3 cut(s) 169, 522, 623
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 5 cut(s) 115, 155, 391, 896, 1079
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 5 cut(s) 128, 307, 492, 519, 1231
Bse3DI GCAATG 3 cut(s) 291, 1237, 1314
BseDI CCNNGG 3 cut(s) 169, 522, 623
BseLI CCNNNNNNNGG 5 cut(s) 115, 155, 391, 896, 1079
BseMI GCAATG 3 cut(s) 291, 1237, 1314
BseMII CTCAG 2 cut(s) 105, 632
BseNI ACTGG 5 cut(s) 128, 307, 492, 519, 1231
BseRI GAGGAG 4 cut(s) 78, 658, 661, 1042
BseSI GKGCMC 1 cut(s) 1226
BseXI GCAGC 2 cut(s) 388, 514
BshFI GGCC 3 cut(s) 202, 491, 998
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 5 cut(s) 115, 155, 391, 896, 1079
BsmAI GTCTC 5 cut(s) 85, 150, 564, 736, 891
BsmI GAATGC 1 cut(s) 286
BsnI GGCC 3 cut(s) 202, 491, 998
Bso31I GGTCTC 1 cut(s) 736
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 2 cut(s) 384, 1226
Bsp143I GATC 5 cut(s) 158, 173, 349, 480, 829
Bsp19I CCATGG 1 cut(s) 169
BspACI CCGC 4 cut(s) 6, 9, 103, 106
BspANI GGCC 3 cut(s) 202, 491, 998
BspCNI CTCAG 2 cut(s) 106, 633
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 1 cut(s) 519
BspMAI CTGCAG 1 cut(s) 251
BspPI GGATC 2 cut(s) 166, 181
BspTI CTTAAG 1 cut(s) 1061
BspTNI GGTCTC 1 cut(s) 736
BsrDI GCAATG 3 cut(s) 291, 1237, 1314
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 5 cut(s) 128, 307, 492, 519, 1231
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 3 cut(s) 169, 522, 623
BssMI GATC 5 cut(s) 158, 173, 349, 480, 829
BssT1I CCWWGG 3 cut(s) 169, 522, 623
Bst4CI ACNGT 5 cut(s) 211, 633, 822, 874, 1131
Bst6I CTCTTC 2 cut(s) 318, 1163
BstAFI CTTAAG 1 cut(s) 1061
BstBAI YACGTR 1 cut(s) 790
BstDEI CTNAG 4 cut(s) 114, 641, 1187, 1331
BstDSI CCRYGG 1 cut(s) 169
BstENI CCTNNNNNAGG 1 cut(s) 113
BstKTI GATC 5 cut(s) 161, 176, 352, 483, 832
BstMAI GTCTC 5 cut(s) 85, 150, 564, 736, 891
BstMBI GATC 5 cut(s) 158, 173, 349, 480, 829
BstMWI GCNNNNNNNGC 2 cut(s) 26, 105
BstNSI RCATGY 2 cut(s) 444, 507
BstSFI CTRYAG 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 1226
BstV1I GCAGC 2 cut(s) 388, 514
BstV2I GAAGAC 1 cut(s) 919
BstX2I RGATCY 3 cut(s) 158, 173, 349
BstYI RGATCY 3 cut(s) 158, 173, 349
BsuRI GGCC 3 cut(s) 202, 491, 998
BtgI CCRYGG 1 cut(s) 169
BtsIMutI CAGTG 4 cut(s) 22, 870, 1168, 1224
CciI TCATGA 1 cut(s) 1318
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 200, 611
Csp6I GTAC 2 cut(s) 166, 1096
CviAII CATG 5 cut(s) 170, 441, 504, 1319, 1358
CviQI GTAC 2 cut(s) 166, 1096
DdeI CTNAG 4 cut(s) 114, 641, 1187, 1331
DpnI GATC 5 cut(s) 160, 175, 351, 482, 831
DpnII GATC 5 cut(s) 158, 173, 349, 480, 829
EaeI YGGCCR 1 cut(s) 996
Eam1104I CTCTTC 2 cut(s) 318, 1163
EarI CTCTTC 2 cut(s) 318, 1163
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 3 cut(s) 169, 522, 623
Eco31I GGTCTC 1 cut(s) 736
Eco47I GGWCC 1 cut(s) 611
Eco57I CTGAAG 3 cut(s) 299, 1028, 1144
Eco88I CYCGRG 1 cut(s) 197
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoT14I CCWWGG 3 cut(s) 169, 522, 623
ErhI CCWWGG 3 cut(s) 169, 522, 623
FaeI CATG 5 cut(s) 173, 444, 507, 1322, 1361
FatI CATG 5 cut(s) 169, 440, 503, 1318, 1357
FblI GTMKAC 1 cut(s) 1038
Fnu4HI GCNGC 5 cut(s) 7, 10, 106, 402, 528
Fsp4HI GCNGC 5 cut(s) 7, 10, 106, 402, 528
FspBI CTAG 2 cut(s) 347, 624
GluI GCNGC 5 cut(s) 7, 10, 106, 402, 528
HaeIII GGCC 3 cut(s) 202, 491, 998
HapII CCGG 1 cut(s) 328
Hin1II CATG 5 cut(s) 173, 444, 507, 1322, 1361
HincII GTYRAC 3 cut(s) 456, 689, 1363
HindII GTYRAC 3 cut(s) 456, 689, 1363
HinfI GANTC 7 cut(s) 310, 421, 574, 592, 1085, 1143, 1354
HpaII CCGG 1 cut(s) 328
HphI GGTGA 4 cut(s) 85, 665, 1036, 1300
Hpy166II GTNNAC 4 cut(s) 456, 689, 1039, 1363
Hpy188I TCNGA 5 cut(s) 309, 718, 752, 941, 1135
Hpy188III TCNNGA 4 cut(s) 353, 614, 952, 1319
Hpy8I GTNNAC 4 cut(s) 456, 689, 1039, 1363
HpyAV CCTTC 3 cut(s) 218, 396, 1083
HpyCH4III ACNGT 5 cut(s) 211, 633, 822, 874, 1131
HpyCH4IV ACGT 1 cut(s) 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 105
HpyF3I CTNAG 4 cut(s) 114, 641, 1187, 1331
HpySE526I ACGT 1 cut(s) 789
Hsp92II CATG 5 cut(s) 173, 444, 507, 1322, 1361
Kzo9I GATC 5 cut(s) 158, 173, 349, 480, 829
LmnI GCTCC 2 cut(s) 387, 500
Lsp1109I GCAGC 2 cut(s) 388, 514
LweI GCATC 3 cut(s) 21, 1021, 1188
MaeI CTAG 2 cut(s) 347, 624
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 2 cut(s) 119, 342
MalI GATC 5 cut(s) 160, 175, 351, 482, 831
MboI GATC 5 cut(s) 158, 173, 349, 480, 829
MboII GAAGA 6 cut(s) 233, 305, 674, 919, 940, 1150
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 3 cut(s) 158, 173, 349
MhlI GDGCHC 2 cut(s) 384, 1226
MlsI TGGCCA 1 cut(s) 998
MluCI AATT 6 cut(s) 465, 472, 582, 943, 1237, 1257
MluNI TGGCCA 1 cut(s) 998
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 730
Mox20I TGGCCA 1 cut(s) 998
MroXI GAANNNNTTC 1 cut(s) 425
MscI TGGCCA 1 cut(s) 998
MseI TTAA 4 cut(s) 708, 1062, 1122, 1372
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 1061
MspI CCGG 1 cut(s) 328
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 286
MwoI GCNNNNNNNGC 2 cut(s) 26, 105
NcoI CCATGG 1 cut(s) 169
NdeII GATC 5 cut(s) 158, 173, 349, 480, 829
NlaIII CATG 5 cut(s) 173, 444, 507, 1322, 1361
NlaIV GGNNCC 1 cut(s) 519
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 2 cut(s) 444, 507
PagI TCATGA 1 cut(s) 1318
PctI GAATGC 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 6 cut(s) 421, 574, 592, 1085, 1143, 1354
PkrI GCNGC 5 cut(s) 8, 11, 107, 403, 529
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Ppu21I YACGTR 1 cut(s) 790
PshBI ATTAAT 1 cut(s) 708
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 2 cut(s) 200, 611
PstI CTGCAG 1 cut(s) 251
PsuI RGATCY 3 cut(s) 158, 173, 349
RsaI GTAC 2 cut(s) 167, 1097
RsaNI GTAC 2 cut(s) 166, 1096
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 4 cut(s) 708, 1062, 1122, 1372
SatI GCNGC 5 cut(s) 7, 10, 106, 402, 528
Sau3AI GATC 5 cut(s) 158, 173, 349, 480, 829
Sau96I GGNCC 2 cut(s) 200, 611
SchI GAGTC 1 cut(s) 304
SduI GDGCHC 2 cut(s) 384, 1226
SfaNI GCATC 3 cut(s) 21, 1021, 1188
SfcI CTRYAG 1 cut(s) 247
SinI GGWCC 1 cut(s) 611
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 673, 1061
SmoI CTYRAG 2 cut(s) 673, 1061
Sse9I AATT 6 cut(s) 465, 472, 582, 943, 1237, 1257
SsiI CCGC 4 cut(s) 6, 9, 103, 106
SspI AATATT 1 cut(s) 973
SspMI CTAG 2 cut(s) 347, 624
StyI CCWWGG 3 cut(s) 169, 522, 623
TaaI ACNGT 5 cut(s) 211, 633, 822, 874, 1131
TaiI ACGT 1 cut(s) 792
TaqI TCGA 1 cut(s) 930
TasI AATT 6 cut(s) 465, 472, 582, 943, 1237, 1257
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 3 cut(s) 9, 12, 108
TfiI GAWTC 6 cut(s) 421, 574, 592, 1085, 1143, 1354
Tru1I TTAA 4 cut(s) 708, 1062, 1122, 1372
Tru9I TTAA 4 cut(s) 708, 1062, 1122, 1372
TscAI CASTG 4 cut(s) 22, 877, 1168, 1231
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 2 cut(s) 401, 527
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 4 cut(s) 853, 983, 1140, 1346
TspGWI ACGGA 1 cut(s) 1008
TspRI CASTG 4 cut(s) 22, 877, 1168, 1231
Vha464I CTTAAG 1 cut(s) 1061
VpaK11BI GGWCC 1 cut(s) 611
VspI ATTAAT 1 cut(s) 708
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 3 cut(s) 472, 943, 1237
XceI RCATGY 2 cut(s) 444, 507
XmaJI CCTAGG 1 cut(s) 623
XmiI GTMKAC 1 cut(s) 1038
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 347, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.