RLG00000003814

Phosphatidylinositol glycan anchor biosynthesis class U

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009567
Physical Location & Seq
Forward (+)
53035339 .. 53040309
4971 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003814

Sequence Viewer

Length: 1425 bp
ATGGAGGAGCAAAGCACGAAGCAGAAGCAGAAGCAGAAGCAGGGAAGGTTCTGGACATGGGTCACAGCTTCAGTGTTATTCAGACTCATTCTCATCTACTTCCCCCAAAATCTCAACCTCTCTTCTCGCCCCGAAGTCTCCACTCCTCTCACCAGCCTTCGTCGCCTGGCCGAAGGATACTGGTTGAAGCAATCATCCATGTCTCCCTACGCAGGATCTATGTACCACGGATCTCCTCTGTTACTCTCACTTCTTGGCCCACTCACAATCAAACGAATTGACGGGCAGCCCAGTCATCTTCTATGCAGTTTGGTTTTTGTGGCTGCAGATATATTGAATTCATTGCTCATTCGTGCTACTGGTCAGACTCTTCAGATTGCATATAGGCAGAGTTTGAGATCACTAGATCTTGACATGATATCAGAAATGGAGATTCTCCCTTCGGGGGATATTGCTGCTTTAATCTACTTGTGGAACCCTTTTACAATAGCTGCATGTGTGGGGTTGTCAACATCTCCAATTGAGAACCTGGCCATCATGTTAGCCCTCTATGGAGCATGTAAACGACTGGTTCCCTTGGCTGCTTTTGGATGGGTCATGGCAACCCATCTGTCTCTTTATCCTATAATTCTTATTATTCCGATTATTCTCTTATTAGGTAATGGTCCAGATACTCCTAGGAAATTGTTCCTGCTGAGGAGACATGGTAAAGTTGAAGAAAACTCCTCGAGTGATAGTTGTCAACAGGAGAAAGTTATTGATCAATCAAAACTACTCGATGTCTTTTCTTGGAGACCTGTCATGCTTTTCTTAGTGTGGACTGTTATGTGGTCAGTCTATTTGTTACTTCTATGTGGCGTATCCGTTAAACAGTATGGAGGTCTTTGGGAGATGTTTACAAGTATGTACGGTTTCATTCTCACAGTACAAGATTTGTCTCCAAACATTGGTGTCTTATGGTATTTCTTTGCCGAGGTCTTTGATTTTTTCAGAAATTTTTTCCTGATTGTATTCCATATGAACATTCTGTTTATGATATTCCCATTGGCCATACGGCTGCACCACCGCCCCTGCTTTCTTGCTTTTGTCTACATTACAATCTCTTCAATGCTCAAGTCTTATCCTTCTGTTGGGGATTCAGCTCTGTACTTTGGTTTGTTGGGCTGGTTTGTTTACGAACTGTCAGAAATGAAATTCTCGTTCTTCCTCTTCTGTGTTTATATTGGGGTTTCCCTCCTTAGCCCTGTGATGCACAACTTATGGATATGGAGGGGAACAGGAAATGCAAATTTCTACTTTGCCACTGCAATGGCTTATGCGTGTTTGCAGATTGTTTTGGTGGTTGAAAGTGTAAGTGCAATGCTCAATCACGACAGGAAGTTGAGAAAGCTATCAAGTGCAAGGCTTCAAGATGGCAAATCATAA

Protein Analysis

475

Amino Acids

53.95

Weight (kDa)

9.06

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-U PF06728 23 - 439 9.2e-113 GPI transamidase subunit PIG-U
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12730 AT1G12730 AT1G63110 AT1G63110 AT1G63110
fragaria_vesca FvH4_5g01570 FvH4_5g01570
malus_domestica MD04G1066500.v1.1 MD06G1073700.v1.1
prunus_persica Prupe.5G091900_v2.0.a1
pyrus_communis pycom04g05980 pycom06g07290
rosa_chinensis RchiOBHm_Chr7g0199111
rosa_laevigata RLG00000003814
rosa_multiflora Rmu_sc0006266.1_g000049
rosa_roxburghii Rroxscaffold_3G00257070
rosa_rugosa Rorug07G0052700 Rorug07G0052800
rosa_samantha Rh7BG179700 Rh7DG179200
rosa_wichuraiana Rw7G015460 Rw7G015490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 947
AccI GTMKAC 1 cut(s) 1089
AciI CCGC 1 cut(s) 1066
AclWI GGATC 2 cut(s) 223, 238
AcoI YGGCCR 3 cut(s) 168, 531, 1047
AcsI RAATTY 4 cut(s) 337, 994, 1193, 1288
AcuI CTGAAG 2 cut(s) 54, 356
AfaI GTAC 4 cut(s) 224, 908, 927, 1148
AfiI CCNNNNNNNGG 4 cut(s) 212, 445, 947, 1130
AgsI TTSAA 6 cut(s) 187, 337, 716, 1107, 1346, 1409
AjnI CCWGG 2 cut(s) 165, 528
AluBI AGCT 4 cut(s) 68, 491, 1142, 1390
AluI AGCT 4 cut(s) 68, 491, 1142, 1390
Alw26I GTCTC 6 cut(s) 142, 207, 618, 694, 787, 942
AlwI GGATC 2 cut(s) 223, 238
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 4 cut(s) 168, 256, 531, 1047
ApeKI GCWGC 6 cut(s) 286, 323, 455, 491, 581, 1057
ApoI RAATTY 4 cut(s) 337, 994, 1193, 1288
Asp700I GAANNNNTTC 1 cut(s) 686
AspA2I CCTAGG 1 cut(s) 677
AspS9I GGNCC 2 cut(s) 257, 665
AsuHPI GGTGA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 727
AvaII GGWCC 1 cut(s) 665
AvrII CCTAGG 1 cut(s) 677
BalI TGGCCA 2 cut(s) 533, 1049
BbvCI CCTCAGC 1 cut(s) 695
BbvI GCAGC 6 cut(s) 298, 310, 442, 478, 568, 1044
BccI CCATC 4 cut(s) 542, 585, 615, 1406
BceAI ACGGC 1 cut(s) 1070
BciT130I CCWGG 2 cut(s) 167, 530
BciVI GTATCC 2 cut(s) 170, 871
BclI TGATCA 1 cut(s) 760
BcoDI GTCTC 6 cut(s) 142, 207, 618, 694, 787, 942
BfaI CTAG 2 cut(s) 404, 678
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 2 cut(s) 170, 871
BglII AGATCT 1 cut(s) 406
BisI GCNGC 6 cut(s) 287, 324, 456, 492, 582, 1058
BlnI CCTAGG 1 cut(s) 677
BlsI GCNGC 6 cut(s) 288, 325, 457, 493, 583, 1059
Bme1390I CCNGG 2 cut(s) 167, 530
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 1 cut(s) 727
BmgT120I GGNCC 2 cut(s) 257, 665
BmiI GGNNCC 2 cut(s) 476, 573
BmrFI CCNGG 2 cut(s) 167, 530
BmrI ACTGGG 1 cut(s) 285
BmsI GCATC 1 cut(s) 1239
BmuI ACTGGG 1 cut(s) 285
BoxI GACNNNNGTC 1 cut(s) 59
Bpu10I CCTNAGC 2 cut(s) 695, 1238
BpuEI CTTGAG 1 cut(s) 1097
BsaI GGTCTC 1 cut(s) 787
BsaJI CCNNGG 4 cut(s) 226, 576, 677, 972
BsaXI ACNNNNNCTCC 2 cut(s) 691, 721
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 445, 947, 1130
Bse1I ACTGG 4 cut(s) 185, 291, 364, 573
Bse3DI GCAATG 3 cut(s) 341, 1314, 1365
BseBI CCWGG 2 cut(s) 167, 530
BseDI CCNNGG 4 cut(s) 226, 576, 677, 972
BseGI GGATG 2 cut(s) 194, 596
BseLI CCNNNNNNNGG 4 cut(s) 212, 445, 947, 1130
BseMI GCAATG 3 cut(s) 341, 1314, 1365
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 4 cut(s) 185, 291, 364, 573
BseRI GAGGAG 5 cut(s) 20, 135, 225, 712, 715
BseXI GCAGC 6 cut(s) 298, 310, 442, 478, 568, 1044
BsgI GTGCAG 1 cut(s) 1043
BshFI GGCC 4 cut(s) 170, 258, 533, 1049
BsiHKCI CYCGRG 1 cut(s) 727
BslI CCNNNNNNNGG 4 cut(s) 212, 445, 947, 1130
BsmAI GTCTC 6 cut(s) 142, 207, 618, 694, 787, 942
BsnI GGCC 4 cut(s) 170, 258, 533, 1049
Bso31I GGTCTC 1 cut(s) 787
BsoBI CYCGRG 1 cut(s) 727
Bsp143I GATC 5 cut(s) 215, 230, 398, 406, 760
BspACI CCGC 1 cut(s) 1066
BspANI GGCC 4 cut(s) 170, 258, 533, 1049
BspCNI CTCAG 1 cut(s) 687
BspLI GGNNCC 2 cut(s) 476, 573
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 2 cut(s) 223, 238
BspTNI GGTCTC 1 cut(s) 787
BsrDI GCAATG 3 cut(s) 341, 1314, 1365
BsrI ACTGG 4 cut(s) 185, 291, 364, 573
BssECI CCNNGG 4 cut(s) 226, 576, 677, 972
BssMI GATC 5 cut(s) 215, 230, 398, 406, 760
BssT1I CCWWGG 2 cut(s) 576, 677
Bst2UI CCWGG 2 cut(s) 167, 530
Bst4CI ACNGT 5 cut(s) 823, 873, 911, 925, 1182
Bst6I CTCTTC 4 cut(s) 127, 375, 1108, 1214
BstDEI CTNAG 3 cut(s) 695, 811, 1238
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 2 cut(s) 194, 596
BstKTI GATC 5 cut(s) 218, 233, 401, 409, 763
BstMAI GTCTC 6 cut(s) 142, 207, 618, 694, 787, 942
BstMBI GATC 5 cut(s) 215, 230, 398, 406, 760
BstMWI GCNNNNNNNGC 1 cut(s) 162
BstNI CCWGG 2 cut(s) 167, 530
BstNSI RCATGY 2 cut(s) 498, 561
BstPAI GACNNNNGTC 1 cut(s) 59
BstSCI CCNGG 2 cut(s) 165, 528
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 6 cut(s) 298, 310, 442, 478, 568, 1044
BstX2I RGATCY 3 cut(s) 215, 230, 406
BstXI CCANNNNNNTGG 1 cut(s) 1309
BstYI RGATCY 3 cut(s) 215, 230, 406
BsuI GTATCC 2 cut(s) 170, 871
BsuRI GGCC 4 cut(s) 170, 258, 533, 1049
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 2 cut(s) 194, 596
BtsI GCAGTG 1 cut(s) 1302
BtsIMutI CAGTG 2 cut(s) 78, 1302
Cfr13I GGNCC 2 cut(s) 257, 665
Csp6I GTAC 4 cut(s) 223, 907, 926, 1147
CviAII CATG 9 cut(s) 57, 199, 415, 495, 538, 558, 598, 704, 802
CviQI GTAC 4 cut(s) 223, 907, 926, 1147
DdeI CTNAG 3 cut(s) 695, 811, 1238
DpnI GATC 5 cut(s) 217, 232, 400, 408, 762
DpnII GATC 5 cut(s) 215, 230, 398, 406, 760
EaeI YGGCCR 3 cut(s) 168, 531, 1047
Eam1104I CTCTTC 4 cut(s) 127, 375, 1108, 1214
EarI CTCTTC 4 cut(s) 127, 375, 1108, 1214
Eco130I CCWWGG 2 cut(s) 576, 677
Eco31I GGTCTC 1 cut(s) 787
Eco32I GATATC 1 cut(s) 420
Eco47I GGWCC 1 cut(s) 665
Eco57I CTGAAG 2 cut(s) 54, 356
Eco88I CYCGRG 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 2 cut(s) 165, 528
EcoRV GATATC 1 cut(s) 420
EcoT14I CCWWGG 2 cut(s) 576, 677
ErhI CCWWGG 2 cut(s) 576, 677
FaeI CATG 9 cut(s) 60, 202, 418, 498, 541, 561, 601, 707, 805
FatI CATG 9 cut(s) 56, 198, 414, 494, 537, 557, 597, 703, 801
FauNDI CATATG 1 cut(s) 1017
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 1 cut(s) 1089
Fnu4HI GCNGC 6 cut(s) 287, 324, 456, 492, 582, 1058
FokI GGATG 2 cut(s) 181, 603
Fsp4HI GCNGC 6 cut(s) 287, 324, 456, 492, 582, 1058
FspBI CTAG 2 cut(s) 404, 678
GluI GCNGC 6 cut(s) 287, 324, 456, 492, 582, 1058
HaeIII GGCC 4 cut(s) 170, 258, 533, 1049
Hin1II CATG 9 cut(s) 60, 202, 418, 498, 541, 561, 601, 707, 805
HincII GTYRAC 2 cut(s) 510, 743
HindII GTYRAC 2 cut(s) 510, 743
HinfI GANTC 4 cut(s) 84, 367, 433, 1136
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 7 cut(s) 510, 563, 743, 819, 897, 1090, 1174
Hpy188I TCNGA 7 cut(s) 83, 366, 375, 424, 642, 992, 1186
Hpy188III TCNNGA 6 cut(s) 52, 410, 668, 1003, 1370, 1409
Hpy8I GTNNAC 7 cut(s) 510, 563, 743, 819, 897, 1090, 1174
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 5 cut(s) 39, 167, 167, 450, 1134
HpyCH4III ACNGT 5 cut(s) 823, 873, 911, 925, 1182
HpyF10VI GCNNNNNNNGC 1 cut(s) 162
HpyF3I CTNAG 3 cut(s) 695, 811, 1238
Hsp92II CATG 9 cut(s) 60, 202, 418, 498, 541, 561, 601, 707, 805
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 5 cut(s) 215, 230, 398, 406, 760
LmnI GCTCC 2 cut(s) 7, 554
Lsp1109I GCAGC 6 cut(s) 298, 310, 442, 478, 568, 1044
LweI GCATC 1 cut(s) 1239
MaeI CTAG 2 cut(s) 404, 678
MaeIII GTNAC 3 cut(s) 61, 240, 843
MalI GATC 5 cut(s) 217, 232, 400, 408, 762
MboI GATC 5 cut(s) 215, 230, 398, 406, 760
MboII GAAGA 7 cut(s) 114, 290, 362, 728, 1095, 1195, 1201
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 3 cut(s) 215, 230, 406
MlsI TGGCCA 2 cut(s) 533, 1049
MluCI AATT 8 cut(s) 276, 337, 519, 627, 683, 994, 1193, 1288
MluNI TGGCCA 2 cut(s) 533, 1049
MlyI GAGTC 2 cut(s) 78, 361
Mox20I TGGCCA 2 cut(s) 533, 1049
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 2 cut(s) 533, 1049
MseI TTAA 2 cut(s) 461, 867
MslI CAYNNNNRTG 1 cut(s) 1307
Msp20I TGGCCA 2 cut(s) 533, 1049
MspR9I CCNGG 2 cut(s) 167, 530
MunI CAATTG 1 cut(s) 519
MvaI CCWGG 2 cut(s) 167, 530
MwoI GCNNNNNNNGC 1 cut(s) 162
NdeI CATATG 1 cut(s) 1017
NdeII GATC 5 cut(s) 215, 230, 398, 406, 760
NlaIII CATG 9 cut(s) 60, 202, 418, 498, 541, 561, 601, 707, 805
NlaIV GGNNCC 2 cut(s) 476, 573
NmeAIII GCCGAG 1 cut(s) 997
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 2 cut(s) 498, 561
PaeR7I CTCGAG 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 2 cut(s) 433, 1136
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 6 cut(s) 288, 325, 457, 493, 583, 1059
PleI GAGTC 2 cut(s) 78, 361
PpsI GAGTC 2 cut(s) 78, 361
PshAI GACNNNNGTC 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 165, 528
PspGI CCWGG 2 cut(s) 165, 528
PspN4I GGNNCC 2 cut(s) 476, 573
PspPI GGNCC 2 cut(s) 257, 665
PspXI VCTCGAGB 1 cut(s) 727
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 3 cut(s) 215, 230, 406
RsaI GTAC 4 cut(s) 224, 908, 927, 1148
RsaNI GTAC 4 cut(s) 223, 907, 926, 1147
RseI CAYNNNNRTG 1 cut(s) 1307
SaqAI TTAA 2 cut(s) 461, 867
SatI GCNGC 6 cut(s) 287, 324, 456, 492, 582, 1058
Sau3AI GATC 5 cut(s) 215, 230, 398, 406, 760
Sau96I GGNCC 2 cut(s) 257, 665
SchI GAGTC 2 cut(s) 78, 361
ScrFI CCNGG 2 cut(s) 167, 530
SfaNI GCATC 1 cut(s) 1239
SfcI CTRYAG 1 cut(s) 324
Sfr274I CTCGAG 1 cut(s) 727
SinI GGWCC 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 727
SmiMI CAYNNNNRTG 1 cut(s) 1307
SmlI CTYRAG 2 cut(s) 727, 1112
SmoI CTYRAG 2 cut(s) 727, 1112
Sse9I AATT 8 cut(s) 276, 337, 519, 627, 683, 994, 1193, 1288
SsiI CCGC 1 cut(s) 1066
SspMI CTAG 2 cut(s) 404, 678
StyD4I CCNGG 2 cut(s) 165, 528
StyI CCWWGG 2 cut(s) 576, 677
TaaI ACNGT 5 cut(s) 823, 873, 911, 925, 1182
TaqI TCGA 2 cut(s) 728, 777
TasI AATT 8 cut(s) 276, 337, 519, 627, 683, 994, 1193, 1288
TatI WGTACW 2 cut(s) 925, 1146
TfiI GAWTC 2 cut(s) 433, 1136
Tru1I TTAA 2 cut(s) 461, 867
Tru9I TTAA 2 cut(s) 461, 867
TscAI CASTG 2 cut(s) 78, 1309
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 6 cut(s) 286, 323, 455, 491, 581, 1057
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 330, 904, 1034, 1205
TspGWI ACGGA 2 cut(s) 243, 853
TspRI CASTG 2 cut(s) 78, 1309
Van91I CCANNNNNTGG 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 4 cut(s) 337, 994, 1193, 1288
XceI RCATGY 2 cut(s) 498, 561
XhoI CTCGAG 1 cut(s) 727
XmaJI CCTAGG 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 1089
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 2 cut(s) 404, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.