Rorug07G0052800

Phosphatidylinositol glycan anchor biosynthesis class U

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009394
Physical Location & Seq
Forward (+)
3749994 .. 3752039
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0052800.1

Sequence Viewer

Length: 879 bp
ATGGACCGGTCCGTCACCTTCGAAACCCTCCGGTTCAAGACGAGCCTCGTCAGCTCCGCTTCGCCGAGTTGCGTAGAGATCAAGTACCAGCTTCCCAACGAGACTCTCGAGGCTCGGCTCGTCTCCGTCGCGGCGATGATCAGCGAGTTCGAGGCCTGGCAGAGGATTCCGGTTTACTTGTTTGATCGCACCGCCGGTTCGGCTTGCAGAGAGAATGCGGTTCGGCTTGATCCACACGGCTTCATTGATGCAGAGGAGGATCTAATATCACTTCCTAGGGTGGGTGATGATTATCCTCAGGCTTCAGTGAATAGCGAGACCAGCCCTGCAACATCGGAAGTTGTAGTTAATGGAAGTGATAGTCAAACTCTGGCTGTTGGCCAGGAATTCTCAGATACAAAAGCATTTCGTGATGCATTGGTAGCTACGGCTATTGCCAAGAAGTTTGAGTTAACATTTATCAGATCCGACCGAGCGCGAGTAACTGCTAAATGTGCTGAAGATGGCTGCTCATGGCGTATACATGCATCCAAGCTTCCAGATGTTGACACTTTCCAGATAAAGGCATTGAAGGGAGTGCATTGTTGTGTACTTCCAGAACGATCTGGCCACCGACAAGCAAAAATGAAATGGATCTTAAATTGTATCATGGATCGTGTGCGAGAAAATATCAACTACAAACCTAAGGAAATCATAAAGGACATTGAGCAAGAGTATGGGGTTATTATACCATATTTGAAGGCCCATCGAGCTAAAGAGCGAGCCTTGGAGTTGATATATGGAATGCCAATGAAGGACATAGTTTTTAATCAAGCTGAAGTTCAGGGAGTAGAGAAGGCCTTGCATGATGTACTTGTCATTGTAGGAAGAAGTCTTTGA

Protein Analysis

292

Amino Acids

32.74

Weight (kDa)

6.13

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 123 - 187 1.3e-22 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AccI GTMKAC 1 cut(s) 520
AccII CGCG 2 cut(s) 131, 478
AciI CCGC 4 cut(s) 57, 131, 192, 218
AclWI GGATC 5 cut(s) 224, 267, 459, 641, 660
AcoI YGGCCR 2 cut(s) 379, 607
AcsI RAATTY 1 cut(s) 386
AcuI CTGAAG 3 cut(s) 288, 519, 837
AfaI GTAC 3 cut(s) 86, 591, 852
AfiI CCNNNNNNNGG 3 cut(s) 162, 281, 562
AgeI ACCGGT 1 cut(s) 6
AgsI TTSAA 3 cut(s) 37, 571, 739
AjnI CCWGG 2 cut(s) 155, 381
AluBI AGCT 6 cut(s) 54, 91, 425, 535, 752, 815
AluI AGCT 6 cut(s) 54, 91, 425, 535, 752, 815
Alw26I GTCTC 3 cut(s) 95, 127, 311
AlwI GGATC 5 cut(s) 224, 267, 459, 641, 660
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 5 cut(s) 153, 379, 607, 741, 837
ApeKI GCWGC 1 cut(s) 507
ApoI RAATTY 1 cut(s) 386
AsiGI ACCGGT 1 cut(s) 6
AspA2I CCTAGG 1 cut(s) 275
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 3 cut(s) 4, 9, 742
AsuHPI GGTGA 2 cut(s) 7, 296
AsuII TTCGAA 1 cut(s) 21
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 2 cut(s) 4, 9
AvrII CCTAGG 1 cut(s) 275
AxyI CCTNAGG 2 cut(s) 297, 684
BalI TGGCCA 2 cut(s) 381, 609
BbvI GCAGC 1 cut(s) 494
BccI CCATC 2 cut(s) 497, 753
BceAI ACGGC 2 cut(s) 253, 444
BciT130I CCWGG 2 cut(s) 157, 383
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 3 cut(s) 95, 127, 311
BfaI CTAG 1 cut(s) 276
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 2 cut(s) 132, 508
BlnI CCTAGG 1 cut(s) 275
BlsI GCNGC 2 cut(s) 133, 509
Bme1390I CCNGG 2 cut(s) 157, 383
Bme18I GGWCC 2 cut(s) 4, 9
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 3 cut(s) 4, 9, 742
BmrFI CCNGG 2 cut(s) 157, 383
BmsI GCATC 3 cut(s) 238, 403, 536
Bpu14I TTCGAA 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 275, 765
BsaWI WCCGGW 3 cut(s) 6, 30, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 281, 562
Bse118I RCCGGY 2 cut(s) 6, 194
Bse21I CCTNAGG 2 cut(s) 297, 684
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 2 cut(s) 157, 383
BseDI CCNNGG 2 cut(s) 275, 765
BseGI GGATG 1 cut(s) 527
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 3 cut(s) 162, 281, 562
BseMII CTCAG 2 cut(s) 311, 405
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 494
Bsh1236I CGCG 2 cut(s) 131, 478
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 5 cut(s) 155, 381, 609, 743, 839
BshTI ACCGGT 1 cut(s) 6
BsiEI CGRYCG 1 cut(s) 472
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 4 cut(s) 7, 31, 170, 195
BslI CCNNNNNNNGG 3 cut(s) 162, 281, 562
BsmAI GTCTC 3 cut(s) 95, 127, 311
BsmBI CGTCTC 1 cut(s) 127
BsmI GAATGC 2 cut(s) 220, 789
BsnI GGCC 5 cut(s) 155, 381, 609, 743, 839
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 21
Bsp143I GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
BspACI CCGC 4 cut(s) 57, 131, 192, 218
BspANI GGCC 5 cut(s) 155, 381, 609, 743, 839
BspCNI CTCAG 2 cut(s) 310, 404
BspFNI CGCG 2 cut(s) 131, 478
BspPI GGATC 5 cut(s) 224, 267, 459, 641, 660
BspT104I TTCGAA 1 cut(s) 21
BspTNI GGTCTC 1 cut(s) 311
BsrFI RCCGGY 2 cut(s) 6, 194
BssAI RCCGGY 2 cut(s) 6, 194
BssECI CCNNGG 2 cut(s) 275, 765
BssMI GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
BssNAI GTATAC 1 cut(s) 521
BssT1I CCWWGG 2 cut(s) 275, 765
Bst1107I GTATAC 1 cut(s) 521
Bst2UI CCWGG 2 cut(s) 157, 383
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 205, 762
BstDEI CTNAG 3 cut(s) 297, 391, 684
BstENI CCTNNNNNAGG 1 cut(s) 160
BstF5I GGATG 1 cut(s) 527
BstFNI CGCG 2 cut(s) 131, 478
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 9 cut(s) 81, 141, 187, 232, 262, 467, 605, 636, 655
BstMAI GTCTC 3 cut(s) 95, 127, 311
BstMBI GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 6 cut(s) 51, 200, 321, 422, 494, 749
BstNI CCWGG 2 cut(s) 157, 383
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 155, 381
BstUI CGCG 2 cut(s) 131, 478
BstV1I GCAGC 1 cut(s) 494
BstX2I RGATCY 3 cut(s) 259, 464, 633
BstYI RGATCY 3 cut(s) 259, 464, 633
BstZ17I GTATAC 1 cut(s) 521
Bsu36I CCTNAGG 2 cut(s) 297, 684
BsuRI GGCC 5 cut(s) 155, 381, 609, 743, 839
BtgZI GCGATG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 205, 762
CfoI GCGC 1 cut(s) 478
Cfr10I RCCGGY 2 cut(s) 6, 194
Cfr13I GGNCC 3 cut(s) 4, 9, 742
CpoI CGGWCCG 1 cut(s) 9
Csp6I GTAC 3 cut(s) 85, 590, 851
CspAI ACCGGT 1 cut(s) 6
CspI CGGWCCG 1 cut(s) 9
CviAII CATG 4 cut(s) 513, 524, 649, 845
CviQI GTAC 3 cut(s) 85, 590, 851
DdeI CTNAG 3 cut(s) 297, 391, 684
DpnI GATC 9 cut(s) 80, 140, 186, 231, 261, 466, 604, 635, 654
DpnII GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
EaeI YGGCCR 2 cut(s) 379, 607
Eco130I CCWWGG 2 cut(s) 275, 765
Eco147I AGGCCT 2 cut(s) 155, 839
Eco31I GGTCTC 1 cut(s) 311
Eco47I GGWCC 2 cut(s) 4, 9
Eco57I CTGAAG 3 cut(s) 288, 519, 837
Eco81I CCTNAGG 2 cut(s) 297, 684
Eco88I CYCGRG 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 155, 381
EcoT14I CCWWGG 2 cut(s) 275, 765
EcoT22I ATGCAT 2 cut(s) 418, 529
ErhI CCWWGG 2 cut(s) 275, 765
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 4 cut(s) 516, 527, 652, 848
FatI CATG 4 cut(s) 512, 523, 648, 844
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 520
Fnu4HI GCNGC 2 cut(s) 132, 508
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 2 cut(s) 132, 508
FspBI CTAG 1 cut(s) 276
GlaI GCGC 1 cut(s) 477
GluI GCNGC 2 cut(s) 132, 508
HaeIII GGCC 5 cut(s) 155, 381, 609, 743, 839
HapII CCGG 4 cut(s) 7, 31, 170, 195
HhaI GCGC 1 cut(s) 478
Hin1II CATG 4 cut(s) 516, 527, 652, 848
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HincII GTYRAC 2 cut(s) 453, 547
HindII GTYRAC 2 cut(s) 453, 547
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 2 cut(s) 103, 166
HpaI GTTAAC 1 cut(s) 453
HpaII CCGG 4 cut(s) 7, 31, 170, 195
HphI GGTGA 2 cut(s) 7, 296
Hpy166II GTNNAC 5 cut(s) 175, 453, 521, 547, 590
Hpy188I TCNGA 4 cut(s) 337, 394, 464, 469
Hpy188III TCNNGA 6 cut(s) 37, 107, 410, 539, 556, 596
Hpy8I GTNNAC 5 cut(s) 175, 453, 521, 547, 590
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 5 cut(s) 28, 565, 733, 787, 829
HpyCH4V TGCA 7 cut(s) 207, 251, 329, 416, 527, 580, 844
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 200, 321, 422, 494, 749
HpyF3I CTNAG 3 cut(s) 297, 391, 684
Hsp92II CATG 4 cut(s) 516, 527, 652, 848
HspAI GCGC 1 cut(s) 476
Ksp22I TGATCA 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 453
Kzo9I GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 494
LweI GCATC 3 cut(s) 238, 403, 536
MaeI CTAG 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 13, 481
MalI GATC 9 cut(s) 80, 140, 186, 231, 261, 466, 604, 635, 654
MboI GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
MboII GAAGA 2 cut(s) 512, 879
MflI RGATCY 3 cut(s) 259, 464, 633
MlsI TGGCCA 2 cut(s) 381, 609
MluCI AATT 2 cut(s) 386, 640
MluNI TGGCCA 2 cut(s) 381, 609
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 8 cut(s) 38, 56, 103, 145, 156, 247, 250, 306
Mox20I TGGCCA 2 cut(s) 381, 609
Mph1103I ATGCAT 2 cut(s) 418, 529
MscI TGGCCA 2 cut(s) 381, 609
MseI TTAA 4 cut(s) 348, 452, 638, 807
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 2 cut(s) 381, 609
MspI CCGG 4 cut(s) 7, 31, 170, 195
MspR9I CCNGG 2 cut(s) 157, 383
Mva1269I GAATGC 2 cut(s) 220, 789
MvaI CCWGG 2 cut(s) 157, 383
MvnI CGCG 2 cut(s) 131, 478
MwoI GCNNNNNNNGC 6 cut(s) 51, 200, 321, 422, 494, 749
NdeII GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
NlaIII CATG 4 cut(s) 516, 527, 652, 848
NmeAIII GCCGAG 2 cut(s) 90, 94
NmuCI GTSAC 1 cut(s) 13
NsiI ATGCAT 2 cut(s) 418, 529
NspI RCATGY 1 cut(s) 527
NspV TTCGAA 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 107
PceI AGGCCT 2 cut(s) 155, 839
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 2 cut(s) 220, 789
PfeI GAWTC 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 6
PkrI GCNGC 2 cut(s) 133, 509
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 155, 381
PspGI CCWGG 2 cut(s) 155, 381
PspPI GGNCC 3 cut(s) 4, 9, 742
PsuI RGATCY 3 cut(s) 259, 464, 633
RsaI GTAC 3 cut(s) 86, 591, 852
RsaNI GTAC 3 cut(s) 85, 590, 851
RseI CAYNNNNRTG 1 cut(s) 585
Rsr2I CGGWCCG 1 cut(s) 9
RsrII CGGWCCG 1 cut(s) 9
SaqAI TTAA 4 cut(s) 348, 452, 638, 807
SatI GCNGC 2 cut(s) 132, 508
Sau3AI GATC 9 cut(s) 78, 138, 184, 229, 259, 464, 602, 633, 652
Sau96I GGNCC 3 cut(s) 4, 9, 742
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 2 cut(s) 157, 383
SetI ASST 8 cut(s) 20, 56, 93, 427, 537, 685, 754, 817
SfaNI GCATC 3 cut(s) 238, 403, 536
Sfr274I CTCGAG 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 2 cut(s) 4, 9
SlaI CTCGAG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 2 cut(s) 386, 640
SseBI AGGCCT 2 cut(s) 155, 839
SsiI CCGC 4 cut(s) 57, 131, 192, 218
SspMI CTAG 1 cut(s) 276
StuI AGGCCT 2 cut(s) 155, 839
StyD4I CCNGG 2 cut(s) 155, 381
StyI CCWWGG 2 cut(s) 275, 765
TaqI TCGA 4 cut(s) 21, 108, 150, 748
TaqII GACCGA 1 cut(s) 486
TasI AATT 2 cut(s) 386, 640
TatI WGTACW 2 cut(s) 589, 850
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 4 cut(s) 348, 452, 638, 807
Tru9I TTAA 4 cut(s) 348, 452, 638, 807
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 1 cut(s) 507
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 3 cut(s) 232, 641, 806
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 1 cut(s) 312
VpaK11BI GGWCC 2 cut(s) 4, 9
XagI CCTNNNNNAGG 1 cut(s) 160
XapI RAATTY 1 cut(s) 386
XceI RCATGY 1 cut(s) 527
XhoI CTCGAG 1 cut(s) 107
XmaJI CCTAGG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 520
XspI CTAG 1 cut(s) 276
Zsp2I ATGCAT 2 cut(s) 418, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.