AT1G14205

ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004153
Physical Location & Seq
Forward (+)
4855533 .. 4856762
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14205.1

Sequence Viewer

Length: 510 bp
ATGATACTTGGCATGGCTGCTTTGACTTCTCTTTCTACTGTTCAGTTTAGAGTCTCTGACATTCTCGGAACTCGTTTGAAACCTTATCCCCAGTTTCCAATTCGAATAACAACTATGTCTGTAGTAAAGCCGTTTGTGATTGAAGCGAGAGGGAACACTCGAGTGGAGAGTCCGAAAACTAGGAACAGGAGGAGCAGGAAGAAGTTTACTGGAACACAAACAAAACCAAGACTTTCAGTGTTCTGTTCAGATAAACAACTCTATGCTATGTTGGTTGATGACTTTAACAAAAAGTGTTTGTTCTACGCTAGTACATTGCAGAAGTCCATTCGCGGAGATCCTCCATGTACTGTCATTGAAGCAGCTAAACGGGTTGGAGAAGAGCTCATTAAGGCTTCTATTGACCTCAAGATTAACGAGATATCGTCTTATGATCGAAATGGAAATAAACGAGGCGAAAGAATGCAAGCCTTTGAAATTGCAATTGCGCAACACGGGTTCTTACCGTAG

Protein Analysis

169

Amino Acids

19.1

Weight (kDa)

10.1

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 53 - 161 3.5e-18 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 489
AccII CGCG 1 cut(s) 333
AciI CCGC 1 cut(s) 333
AclWI GGATC 1 cut(s) 332
AfaI GTAC 2 cut(s) 313, 349
AgsI TTSAA 4 cut(s) 79, 143, 359, 476
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 2 cut(s) 365, 385
AluI AGCT 2 cut(s) 365, 385
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 58
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 17, 362
AspLEI GCGC 1 cut(s) 490
AsuII TTCGAA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 159
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 4, 374
BceAI ACGGC 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 2 cut(s) 180, 309
BfmI CTRYAG 1 cut(s) 120
BisI GCNGC 2 cut(s) 18, 363
BlsI GCNGC 2 cut(s) 19, 364
BmeT110I CYCGRG 1 cut(s) 159
BmrI ACTGGG 1 cut(s) 85
BmuI ACTGGG 1 cut(s) 85
BplI GAGNNNNNCTC 2 cut(s) 369, 401
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 91, 214
Bse3DI GCAATG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 91, 214
BseRI GAGGAG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 4, 374
Bsh1236I CGCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 58
BsmI GAATGC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 337, 433
BspACI CCGC 1 cut(s) 333
BspFNI CGCG 1 cut(s) 333
BspPI GGATC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 314
BsrI ACTGG 2 cut(s) 91, 214
BssMI GATC 2 cut(s) 337, 433
Bst4CI ACNGT 3 cut(s) 40, 352, 507
Bst6I CTCTTC 1 cut(s) 375
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 468
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 2 cut(s) 340, 436
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 2 cut(s) 337, 433
BstSFI CTRYAG 1 cut(s) 120
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 4, 374
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 468
CfoI GCGC 1 cut(s) 490
Csp6I GTAC 2 cut(s) 312, 348
CviAII CATG 2 cut(s) 13, 345
CviJI RGCY 6 cut(s) 17, 130, 365, 385, 395, 470
CviKI_1 RGCY 6 cut(s) 17, 130, 365, 385, 395, 470
CviQI GTAC 2 cut(s) 312, 348
DpnI GATC 2 cut(s) 339, 435
DpnII GATC 2 cut(s) 337, 433
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 387
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 385
EcoRV GATATC 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 387
FaeI CATG 2 cut(s) 16, 348
FaiI YATR 6 cut(s) 14, 116, 264, 269, 346, 432
FatI CATG 2 cut(s) 12, 344
Fnu4HI GCNGC 2 cut(s) 18, 363
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 18, 363
FspBI CTAG 2 cut(s) 180, 309
FspI TGCGCA 1 cut(s) 489
GlaI GCGC 1 cut(s) 489
GluI GCNGC 2 cut(s) 18, 363
HhaI GCGC 1 cut(s) 490
Hin1II CATG 2 cut(s) 16, 348
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 2 cut(s) 51, 169
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 4 cut(s) 58, 68, 174, 250
Hpy188III TCNNGA 1 cut(s) 409
Hpy8I GTNNAC 1 cut(s) 207
HpyCH4III ACNGT 3 cut(s) 40, 352, 507
HpyCH4V TGCA 3 cut(s) 319, 466, 482
Hsp92II CATG 2 cut(s) 16, 348
HspAI GCGC 1 cut(s) 488
Kzo9I GATC 2 cut(s) 337, 433
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 4 cut(s) 104, 172, 181, 195
Lsp1109I GCAGC 2 cut(s) 4, 374
MaeI CTAG 2 cut(s) 180, 309
MalI GATC 2 cut(s) 339, 435
MboI GATC 2 cut(s) 337, 433
MboII GAAGA 2 cut(s) 211, 392
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 3 cut(s) 99, 477, 483
MlyI GAGTC 2 cut(s) 60, 178
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 5 cut(s) 143, 183, 351, 416, 446
MseI TTAA 3 cut(s) 285, 390, 414
MslI CAYNNNNRTG 1 cut(s) 161
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 1 cut(s) 468
MvnI CGCG 1 cut(s) 333
NdeII GATC 2 cut(s) 337, 433
NlaIII CATG 2 cut(s) 16, 348
NsbI TGCGCA 1 cut(s) 489
NspV TTCGAA 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 1 cut(s) 468
PkrI GCNGC 2 cut(s) 19, 364
PleI GAGTC 2 cut(s) 59, 177
PpsI GAGTC 2 cut(s) 59, 177
Psp124BI GAGCTC 1 cut(s) 387
PspXI VCTCGAGB 1 cut(s) 159
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 2 cut(s) 313, 349
RsaNI GTAC 2 cut(s) 312, 348
RseI CAYNNNNRTG 1 cut(s) 161
SacI GAGCTC 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 285, 390, 414
SatI GCNGC 2 cut(s) 18, 363
Sau3AI GATC 2 cut(s) 337, 433
SchI GAGTC 2 cut(s) 60, 178
SduI GDGCHC 1 cut(s) 387
SetI ASST 4 cut(s) 85, 367, 387, 408
SfcI CTRYAG 1 cut(s) 120
Sfr274I CTCGAG 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 103
SlaI CTCGAG 1 cut(s) 159
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 2 cut(s) 159, 407
SmoI CTYRAG 2 cut(s) 159, 407
Sse9I AATT 3 cut(s) 99, 477, 483
SsiI CCGC 1 cut(s) 333
SspMI CTAG 2 cut(s) 180, 309
SstI GAGCTC 1 cut(s) 387
TaaI ACNGT 3 cut(s) 40, 352, 507
TaqI TCGA 3 cut(s) 103, 160, 436
TasI AATT 3 cut(s) 99, 477, 483
TatI WGTACW 2 cut(s) 311, 347
Tru1I TTAA 3 cut(s) 285, 390, 414
Tru9I TTAA 3 cut(s) 285, 390, 414
TscAI CASTG 1 cut(s) 243
TseI GCWGC 2 cut(s) 17, 362
TspRI CASTG 1 cut(s) 243
XhoI CTCGAG 1 cut(s) 159
XspI CTAG 2 cut(s) 180, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.