Rh4AG371800

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004153
Physical Location & Seq
Forward (+)
67965697 .. 67967239
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG371800.1

Sequence Viewer

Length: 390 bp
ATGAAGCCGGTAGTAATGAGAGCGAGGCAGCATGCCAGAACCGAAAGTGCGAAAACTCTAAACAGAAGAATGCAGAAGAAGTACAATGGCACGCCTAAGAAGCCGAGGCTTTCAGTATTCTGTTCAGACAAACAATTGTATGCTATGCTGGTAGATGACCAAAACAAGAAGTGTTTGTTTTATGGAAGCACCCTGCAGAAATCTATTCGCCAGGATCCCCATTGTAGTACCCCTGAAGCCGCAAAACGTGTTGGCAAGGAGTTAATCAAGGCCTGCAATGATCTCAACATAGGCGAAATATCGTCTTATGATCGCAATGGGTTTGCTACCGGGGAAAGGATTCAAGCATTTGAGATTGCAATTTCAGAATACGGGTTCTTGTCAAGATAA

Protein Analysis

129

Amino Acids

14.72

Weight (kDa)

9.76

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 13 - 125 6.1e-17 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 240
AclWI GGATC 2 cut(s) 209, 222
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 2 cut(s) 83, 229
AfiI CCNNNNNNNGG 1 cut(s) 336
AflIII ACRYGT 1 cut(s) 247
AgsI TTSAA 1 cut(s) 344
AjnI CCWGG 1 cut(s) 210
AlwI GGATC 2 cut(s) 209, 222
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 331
BamHI GGATCC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 212
BcnI CCSGG 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 194
BisI GCNGC 2 cut(s) 29, 240
BlsI GCNGC 2 cut(s) 30, 241
Bme1390I CCNGG 2 cut(s) 212, 331
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 2 cut(s) 212, 331
BpuMI CCSGG 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 104, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse118I RCCGGY 1 cut(s) 7
Bse3DI GCAATG 2 cut(s) 283, 322
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 104, 330
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMI GCAATG 2 cut(s) 283, 322
BseXI GCAGC 1 cut(s) 40
BshFI GGCC 1 cut(s) 272
BsiSI CCGG 2 cut(s) 8, 330
BslI CCNNNNNNNGG 1 cut(s) 336
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 214, 280, 310
BspACI CCGC 1 cut(s) 240
BspANI GGCC 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 198
BspPI GGATC 2 cut(s) 209, 222
BsrDI GCAATG 2 cut(s) 283, 322
BsrFI RCCGGY 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 2 cut(s) 104, 330
BssMI GATC 3 cut(s) 214, 280, 310
Bst2UI CCWGG 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 33, 92, 274
BstDEI CTNAG 1 cut(s) 96
BstKTI GATC 3 cut(s) 217, 283, 313
BstMBI GATC 3 cut(s) 214, 280, 310
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 2 cut(s) 210, 329
BstSFI CTRYAG 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 1 cut(s) 272
Cac8I GCNNGC 3 cut(s) 33, 92, 274
Cfr10I RCCGGY 1 cut(s) 7
Csp6I GTAC 2 cut(s) 82, 228
CviAII CATG 1 cut(s) 32
CviJI RGCY 5 cut(s) 7, 103, 109, 239, 272
CviKI_1 RGCY 5 cut(s) 7, 103, 109, 239, 272
CviQI GTAC 2 cut(s) 82, 228
DdeI CTNAG 1 cut(s) 96
DpnI GATC 3 cut(s) 216, 282, 312
DpnII GATC 3 cut(s) 214, 280, 310
Eco147I AGGCCT 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 210
FaeI CATG 1 cut(s) 35
FaiI YATR 6 cut(s) 33, 141, 146, 183, 290, 309
FatI CATG 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 29, 240
Fsp4HI GCNGC 2 cut(s) 29, 240
GluI GCNGC 2 cut(s) 29, 240
HaeIII GGCC 1 cut(s) 272
HapII CCGG 2 cut(s) 8, 330
Hin1II CATG 1 cut(s) 35
HinfI GANTC 1 cut(s) 340
HpaII CCGG 2 cut(s) 8, 330
Hpy188I TCNGA 2 cut(s) 127, 367
Hpy188III TCNNGA 1 cut(s) 384
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 4 cut(s) 73, 196, 276, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 1 cut(s) 35
Kzo9I GATC 3 cut(s) 214, 280, 310
LpnPI CCDG 9 cut(s) 21, 49, 134, 197, 206, 224, 246, 286, 343
Lsp1109I GCAGC 1 cut(s) 40
MaeII ACGT 1 cut(s) 247
MalI GATC 3 cut(s) 216, 282, 312
MboI GATC 3 cut(s) 214, 280, 310
MboII GAAGA 2 cut(s) 78, 88
MfeI CAATTG 1 cut(s) 134
MflI RGATCY 1 cut(s) 214
MluCI AATT 2 cut(s) 134, 360
MnlI CCTC 2 cut(s) 18, 99
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 1 cut(s) 263
MspI CCGG 2 cut(s) 8, 330
MspR9I CCNGG 2 cut(s) 212, 331
MunI CAATTG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 331
NdeII GATC 3 cut(s) 214, 280, 310
NlaIII CATG 1 cut(s) 35
NlaIV GGNNCC 1 cut(s) 216
NmeAIII GCCGAG 1 cut(s) 129
NspI RCATGY 1 cut(s) 35
PaeI GCATGC 1 cut(s) 35
PceI AGGCCT 1 cut(s) 272
PctI GAATGC 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 2 cut(s) 30, 241
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 216
PstI CTGCAG 1 cut(s) 198
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 2 cut(s) 83, 229
RsaNI GTAC 2 cut(s) 82, 228
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 2 cut(s) 29, 240
Sau3AI GATC 3 cut(s) 214, 280, 310
ScrFI CCNGG 2 cut(s) 212, 331
SetI ASST 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 194
SphI GCATGC 1 cut(s) 35
Sse9I AATT 2 cut(s) 134, 360
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 1 cut(s) 240
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 210, 329
TaiI ACGT 1 cut(s) 250
TasI AATT 2 cut(s) 134, 360
TatI WGTACW 1 cut(s) 81
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 35
XmnI GAANNNNTTC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.