Rroxscaffold_5G00380640

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004153
Physical Location & Seq
Forward (+)
60805699 .. 60807432
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380640.1

Sequence Viewer

Length: 498 bp
ATGGCTCTAGTGAATGTTGCTGCTCTCCAGTTTACAACTTCTGCCCTTTTTCGGACTCGTTCTGCAACTTCAAACCTTCAGGTCTTGCCGACTAAGAGAAATTTCTCCATGAAGCCGGTAGTAATGAGAGCAAGGCAGCATGCCAGAACCGAAAGTGCGAAAACTCTAAACAGAAGAATGCAGAAGAAGTACAATGGCACGCCTAAGAAGCCGAGGCTTTCAGTATTCTGTTCAGACAAACAATTGTATGCTATGCTGGTTGATGACCAAAACAAGAAGTGTTTGTTTTATGGAAGCACTCTGCAGAAATCTATTCGCCAGGATCCCTACTGTAGTACCCCTGAAGCTGCAAAACGTGTTGGCGAGGAGTTAATCAAGGCCTGCAATGATCTCAACATAGGCGAAATATCGTCTTATGATCGCAATGGGTTTGCTACCGGGGAAAGGATTCACGCATTTGAGATTGCAATTTCAGAATACGGGTTCTTGTCAAGATAA

Protein Analysis

165

Amino Acids

18.69

Weight (kDa)

9.89

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 49 - 161 2.9e-16 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 317, 330
AcsI RAATTY 1 cut(s) 100
AcuI CTGAAG 2 cut(s) 62, 363
AfaI GTAC 2 cut(s) 191, 337
AfiI CCNNNNNNNGG 2 cut(s) 51, 444
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 1 cut(s) 72
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 1 cut(s) 347
AluI AGCT 1 cut(s) 347
AlwI GGATC 2 cut(s) 317, 330
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 3 cut(s) 20, 136, 347
ApoI RAATTY 1 cut(s) 100
Asp700I GAANNNNTTC 1 cut(s) 447
AsuC2I CCSGG 1 cut(s) 439
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BamHI GGATCC 1 cut(s) 322
BbvI GCAGC 3 cut(s) 7, 148, 334
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 1 cut(s) 439
BfaI CTAG 1 cut(s) 8
BfmI CTRYAG 2 cut(s) 302, 331
BisI GCNGC 3 cut(s) 21, 137, 348
BlsI GCNGC 3 cut(s) 22, 138, 349
Bme1390I CCNGG 2 cut(s) 320, 439
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 320, 439
BpmI CTGGAG 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 439
BsaJI CCNNGG 2 cut(s) 212, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 444
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 2 cut(s) 391, 430
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 212, 438
BseLI CCNNNNNNNGG 2 cut(s) 51, 444
BseMI GCAATG 2 cut(s) 391, 430
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 3 cut(s) 7, 148, 334
BshFI GGCC 1 cut(s) 380
BsiSI CCGG 2 cut(s) 116, 438
BslI CCNNNNNNNGG 2 cut(s) 51, 444
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 1 cut(s) 380
Bsp143I GATC 3 cut(s) 322, 388, 418
BspANI GGCC 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 306
BspPI GGATC 2 cut(s) 317, 330
BsrDI GCAATG 2 cut(s) 391, 430
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 2 cut(s) 212, 438
BssMI GATC 3 cut(s) 322, 388, 418
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 332
BstC8I GCNNGC 3 cut(s) 141, 200, 382
BstDEI CTNAG 2 cut(s) 93, 204
BstKTI GATC 3 cut(s) 325, 391, 421
BstMBI GATC 3 cut(s) 322, 388, 418
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 318, 437
BstSFI CTRYAG 2 cut(s) 302, 331
BstV1I GCAGC 3 cut(s) 7, 148, 334
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 1 cut(s) 380
Cac8I GCNNGC 3 cut(s) 141, 200, 382
Cfr10I RCCGGY 1 cut(s) 115
Csp6I GTAC 2 cut(s) 190, 336
CviAII CATG 2 cut(s) 109, 140
CviJI RGCY 6 cut(s) 5, 115, 211, 217, 347, 380
CviKI_1 RGCY 6 cut(s) 5, 115, 211, 217, 347, 380
CviQI GTAC 2 cut(s) 190, 336
DdeI CTNAG 2 cut(s) 93, 204
DpnI GATC 3 cut(s) 324, 390, 420
DpnII GATC 3 cut(s) 322, 388, 418
Eco147I AGGCCT 1 cut(s) 380
Eco57I CTGAAG 2 cut(s) 62, 363
EcoRII CCWGG 1 cut(s) 318
FaeI CATG 2 cut(s) 112, 143
FaiI YATR 7 cut(s) 110, 141, 249, 254, 291, 398, 417
FatI CATG 2 cut(s) 108, 139
Fnu4HI GCNGC 3 cut(s) 21, 137, 348
Fsp4HI GCNGC 3 cut(s) 21, 137, 348
FspBI CTAG 1 cut(s) 8
GluI GCNGC 3 cut(s) 21, 137, 348
GsuI CTGGAG 1 cut(s) 11
HaeIII GGCC 1 cut(s) 380
HapII CCGG 2 cut(s) 116, 438
Hin1II CATG 2 cut(s) 112, 143
HinfI GANTC 2 cut(s) 55, 448
HpaII CCGG 2 cut(s) 116, 438
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 3 cut(s) 54, 235, 475
Hpy188III TCNNGA 1 cut(s) 492
Hpy8I GTNNAC 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 6 cut(s) 65, 181, 304, 350, 384, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpyF3I CTNAG 2 cut(s) 93, 204
HpySE526I ACGT 1 cut(s) 355
Hsp92II CATG 2 cut(s) 112, 143
Kzo9I GATC 3 cut(s) 322, 388, 418
Lsp1109I GCAGC 3 cut(s) 7, 148, 334
MaeI CTAG 1 cut(s) 8
MaeII ACGT 1 cut(s) 355
MalI GATC 3 cut(s) 324, 390, 420
MboI GATC 3 cut(s) 322, 388, 418
MboII GAAGA 2 cut(s) 186, 196
MfeI CAATTG 1 cut(s) 242
MflI RGATCY 1 cut(s) 322
MluCI AATT 3 cut(s) 100, 242, 468
MlyI GAGTC 1 cut(s) 49
MnlI CCTC 2 cut(s) 207, 358
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 1 cut(s) 371
MspI CCGG 2 cut(s) 116, 438
MspR9I CCNGG 2 cut(s) 320, 439
MunI CAATTG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 439
NdeII GATC 3 cut(s) 322, 388, 418
NlaIII CATG 2 cut(s) 112, 143
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 237
NspI RCATGY 1 cut(s) 143
PaeI GCATGC 1 cut(s) 143
PceI AGGCCT 1 cut(s) 380
PctI GAATGC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 3 cut(s) 22, 138, 349
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 306
PsuI RGATCY 1 cut(s) 322
RsaI GTAC 2 cut(s) 191, 337
RsaNI GTAC 2 cut(s) 190, 336
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 3 cut(s) 21, 137, 348
Sau3AI GATC 3 cut(s) 322, 388, 418
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 2 cut(s) 320, 439
SetI ASST 4 cut(s) 78, 84, 349, 358
SfcI CTRYAG 2 cut(s) 302, 331
SphI GCATGC 1 cut(s) 143
Sse9I AATT 3 cut(s) 100, 242, 468
SseBI AGGCCT 1 cut(s) 380
SspMI CTAG 1 cut(s) 8
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 318, 437
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 358
TasI AATT 3 cut(s) 100, 242, 468
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseI GCWGC 3 cut(s) 20, 136, 347
TspDTI ATGAA 1 cut(s) 125
XapI RAATTY 1 cut(s) 100
XceI RCATGY 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.