MD16G1072000.v1.1

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004153
Physical Location & Seq
Forward (+)
5043915 .. 5045602
1688 bp
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UTR
Exon/CDS
Intron
MD16G1072000.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCCCTTGCGAATGCTGCTGCTCTCAAGTTCTCGACTTCTGCATTTTTGGGTCTCCGTTCAGCAACTTCCAACTTTCAAATTCAACCCATGCAGCAGACGAAAAGAACAAGTTTCTCGGCGCAGCCGTTAGTTATGAGAGCGAGGGGGAATGCCAGGACGGATAGTGCGAAAACTCGTAACAGAAGAATGCAGAAGAAGTACAATGGCACGTCTAAGAGACCGAGGCTTTCGGTGTTCTGTTCAGATAAACAGTTGTATGCAATGCTGGTAGATGACCAAAACAAGAACTGTTTGTTTTACGGAAGCACCCTGCAGAAATCTATTCGTCAGAATCCCGATTGTAGTACCAGCGAAGCTGCTAAACGCCTGGGCGAGGAGCTCGTCAAGGCCTGCAACAATCTCAACATACACGAAATATCATCGTATGATCGCAATGGTTTTGCCACTGGGGAAAGGATTCAGGCTTTCGAGATTGCAATTTCTCAGCATGGTTTCTTGCCAAGATAG

Protein Analysis

170

Amino Acids

18.95

Weight (kDa)

10.18

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 53 - 161 2.7e-18 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 81
AfaI GTAC 2 cut(s) 203, 349
AfiI CCNNNNNNNGG 1 cut(s) 376
AgsI TTSAA 2 cut(s) 80, 86
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 155, 369
AluBI AGCT 2 cut(s) 359, 382
AluI AGCT 2 cut(s) 359, 382
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 2 cut(s) 59, 214
AoxI GGCC 2 cut(s) 3, 390
ApeKI GCWGC 5 cut(s) 17, 20, 94, 124, 359
ApoI RAATTY 1 cut(s) 81
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 5 cut(s) 4, 7, 106, 136, 346
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 2 cut(s) 157, 371
BcoDI GTCTC 2 cut(s) 59, 214
BfmI CTRYAG 1 cut(s) 314
BisI GCNGC 5 cut(s) 18, 21, 95, 125, 360
BlsI GCNGC 5 cut(s) 19, 22, 96, 126, 361
Bme1390I CCNGG 2 cut(s) 157, 371
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 2 cut(s) 157, 371
BmrI ACTGGG 1 cut(s) 459
BmuI ACTGGG 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 11
BsaI GGTCTC 2 cut(s) 59, 214
BsaJI CCNNGG 2 cut(s) 224, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 2 cut(s) 270, 442
BseBI CCWGG 2 cut(s) 157, 371
BseDI CCNNGG 2 cut(s) 224, 370
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 2 cut(s) 270, 442
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 5 cut(s) 4, 7, 106, 136, 346
BshFI GGCC 2 cut(s) 5, 392
BsiHKAI GWGCWC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 2 cut(s) 59, 214
BsmI GAATGC 3 cut(s) 19, 157, 195
BsnI GGCC 2 cut(s) 5, 392
Bso31I GGTCTC 2 cut(s) 59, 214
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 1 cut(s) 430
BspANI GGCC 2 cut(s) 5, 392
BspCNI CTCAG 1 cut(s) 499
BspMAI CTGCAG 1 cut(s) 318
BspTNI GGTCTC 2 cut(s) 59, 214
BsrDI GCAATG 2 cut(s) 270, 442
BsrI ACTGG 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 224, 370
BssMI GATC 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 157, 371
Bst4CI ACNGT 2 cut(s) 255, 293
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 216, 486
BstENI CCTNNNNNAGG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 1 cut(s) 433
BstMAI GTCTC 2 cut(s) 59, 214
BstMBI GATC 1 cut(s) 430
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 157, 371
BstSCI CCNGG 2 cut(s) 155, 369
BstSFI CTRYAG 1 cut(s) 314
BstV1I GCAGC 5 cut(s) 4, 7, 106, 136, 346
BsuRI GGCC 2 cut(s) 5, 392
BtrI CACGTC 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 394
CfoI GCGC 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 202, 348
CviAII CATG 2 cut(s) 91, 491
CviJI RGCY 7 cut(s) 5, 127, 229, 359, 382, 392, 467
CviKI_1 RGCY 7 cut(s) 5, 127, 229, 359, 382, 392, 467
CviQI GTAC 2 cut(s) 202, 348
DdeI CTNAG 2 cut(s) 216, 486
DpnI GATC 1 cut(s) 432
DpnII GATC 1 cut(s) 430
Ecl136II GAGCTC 1 cut(s) 382
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 2 cut(s) 59, 214
Eco53kI GAGCTC 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 155, 369
EcoT38I GRGCYC 1 cut(s) 384
FaeI CATG 2 cut(s) 94, 494
FaiI YATR 6 cut(s) 92, 137, 261, 410, 429, 492
FatI CATG 2 cut(s) 90, 490
Fnu4HI GCNGC 5 cut(s) 18, 21, 95, 125, 360
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 5 cut(s) 18, 21, 95, 125, 360
GlaI GCGC 1 cut(s) 123
GluI GCNGC 5 cut(s) 18, 21, 95, 125, 360
HaeIII GGCC 2 cut(s) 5, 392
HhaI GCGC 1 cut(s) 124
Hin1II CATG 2 cut(s) 94, 494
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 2 cut(s) 334, 460
Hpy188I TCNGA 2 cut(s) 247, 333
Hpy188III TCNNGA 3 cut(s) 34, 338, 472
HpyCH4III ACNGT 2 cut(s) 255, 293
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 7 cut(s) 44, 94, 193, 263, 316, 396, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 2 cut(s) 216, 486
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 94, 494
HspAI GCGC 1 cut(s) 122
Kzo9I GATC 1 cut(s) 430
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 5 cut(s) 4, 7, 106, 136, 346
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 179
MalI GATC 1 cut(s) 432
MboI GATC 1 cut(s) 430
MboII GAAGA 2 cut(s) 198, 208
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 2 cut(s) 81, 480
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 3 cut(s) 138, 219, 370
MroXI GAANNNNTTC 1 cut(s) 459
MspR9I CCNGG 2 cut(s) 157, 371
Mva1269I GAATGC 3 cut(s) 19, 157, 195
MvaI CCWGG 2 cut(s) 157, 371
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 1 cut(s) 430
NlaIII CATG 2 cut(s) 94, 494
NmeAIII GCCGAG 1 cut(s) 98
PceI AGGCCT 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 167
PctI GAATGC 3 cut(s) 19, 157, 195
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 334, 460
PkrI GCNGC 5 cut(s) 19, 22, 96, 126, 361
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 155, 369
PspGI CCWGG 2 cut(s) 155, 369
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 318
RsaI GTAC 2 cut(s) 203, 349
RsaNI GTAC 2 cut(s) 202, 348
SacI GAGCTC 1 cut(s) 384
SatI GCNGC 5 cut(s) 18, 21, 95, 125, 360
Sau3AI GATC 1 cut(s) 430
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 157, 371
SduI GDGCHC 1 cut(s) 384
SetI ASST 3 cut(s) 215, 361, 384
SfcI CTRYAG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 2 cut(s) 81, 480
SseBI AGGCCT 1 cut(s) 392
SstI GAGCTC 1 cut(s) 384
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 2 cut(s) 155, 369
TaaI ACNGT 2 cut(s) 255, 293
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 35, 471
TaqII GACCGA 1 cut(s) 238
TasI AATT 2 cut(s) 81, 480
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 2 cut(s) 334, 460
TscAI CASTG 1 cut(s) 454
TseI GCWGC 5 cut(s) 17, 20, 94, 124, 359
TspGWI ACGGA 3 cut(s) 47, 176, 318
TspRI CASTG 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.