Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Forward (+)

6017808 .. 6023503

Gene Structure 5696 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G17500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3651 bp

ATGGCAAGAGGTAGAATAAGGTCAAAGCTGAGACTTAGCCATATATACACATTTGGATGTCTTAGGCCAAGTGCAGATGAAGGTCAAGATCCTCACCCAATCCAAGGTCCAGGATTTAGCAGGACTGTGTACTGTAACCAGCCTCATATGCACAAGAAGAAGCCTTTGAAATACCGTTCTAACTATGTTTCTACCACTAGGTACAATTTGATCACCTTCTTTCCAAAGTGCCTCTACGAACAGTTTCACCGTGCTGCCAATTTCTACTTCTTGGTAGCTGCCATTCTTTCCGTGTTCCCTCTTTCCCCGTTCAACAAATGGAGTATGATTGCTCCACTGGTGTTTGTTGTTGGGCTCAGTATGTTAAAGGAGGCTCTTGAAGATTGGAGTAGGTTTATGCAAGATGTGAAGATCAATGCGAGTAAAGTTTATGTTCATAAAAGCGATGGCGAGTTTCGTCGCAGAAAGTGGAAAAAGATTAGTGTTGGAGATATTGTAAAAGTGGAGAAAGACGGCTTTTTCCCTGCTGATTTGCTCTTGTTGTCGTCCAGTTATGAGGATGGGATTTGTTATGTAGAGACTATGAATTTAGATGGCGAGACAAACTTGAAAGTGAAAAGGTCCTTGGAAGTAACATTATCGTTGGATGACTACGACTCTTTCAAGGATTTCACGGGGATAATAAGATGCGAAGATCCAAACCCCAGCCTTTACACATTTGTTGGCAATCTTGAGTATGAGCGCCAGATATTTCCACTGGATCCAAGTCAGATTCTGCTGAGAGATTCAAAGCTTAGGAATACACCTTATGTTTATGGAGTGGTGGTATTCACAGGGCATGATACTAAGGTGATGCAGAACTCAACAAAATCGCCTTCAAAAAGGAGCAGAATCGAAAAGACGATGGACTATATAATCTACACGCTTCTTGTCCTACTTATTTTGATCTCTTGCATAAGTTCATCGGGATTTGCTTGGGAGACAAAGTTTCACATGCCGAAATGGTGGTACTTAAGACCTGAGGAGCCTGAGAACTTAACCAATCCAAGTAATCCTGTTTATGCTGGATTTGTCCATCTGATCACTGCTCTGCTGCTGTACGGATATTTGATACCGATCTCTCTTTATGTCTCCATTGAGGTTGTGAAAGTGTTGCAAGCATCTTTCATCAATAAAGATCTACACATGTATGATAGTGAGAGTGGGGTTCCAGCCCACGCACGCACATCGAATCTAAACGAAGAGCTAGGACAAGTGGATACTATCCTTTCTGATAAAACGGGAACTTTGACTTGTAATCAGATGGATTTTCTGAAATGTTCAATTGCTGGCACTTCTTATGGTGTACGTTCAAGCGAAGTCGAAGTTGCTGCTGCACAGCAAATGGCTGTTGATCTTGATGAGCATGGAGAAGTATCCAGTAGGACAAGTACTCCAAGGGCTCAGGCTCGAGATATTGAGGTAGAAAGTAGCATTACTCCAAGAATTCCTATAAAGGGGTTTGGTTTCGAGGATATTAGGCTAATGGATGGGAATTGGTTGAGAGAGCCACATACAGATGACATTTTGCTGTTCTTTCGCATATTAGCTATATGTCACACAGCTATTCCCGAGCTTAACGAGGAGACTGGCAAGTACACTTATGAAGCAGAGTCACCAGATGAAGCTTCTTTTCTTACTGCTGCTAGTGAGTTTGGTTTTGTGTTCTTCAAGAGAACCCAATCAAGCGTTTATGTTCATGAAAGGCTATCTCATTCAGGGCAAACTATTGAAAGGGAGTATAAAGTTCTGAATTTGTTGGACTTCACTAGCAAACGAAAGCGAATGTCAGTAGTTGTACGTGACGAGGAAGGGCAGATTCTTCTGCTATGCAAAGGAGCTGACAGTATCATTTTTGAGCGACTGGCTAAAAATGGGAAAGTATACTTGGGACCTACCACTAAGCATTTAAATGAATATGGAGAAGCAGGACTCCGTACTCTTGCACTTTCATACAGAAAGCTTGACGAGGAAGAATATTCAGCTTGGAACGCTGAGTTTCACAAGGCCAAAACTTCTATAGGTTCGGATAGAGATGAGTTGCTTGAGAGGATATCGGATATGATCGAAAAAGACCTTATTCTTGTAGGTGCAACTGCTGTGGAGGACAAACTCCAGAAAGGGGTGCCCCAATGCATTGATAAACTTGCCCAAGCTGGCCTCAAATTATGGGTTTTAACTGGGGATAAGATGGAAACAGCAATCAACATCGGATACTCGTGTAGTTTACTTCGGCAAGGCATGAAACAGATATGCATAACTGTGGTGAACTCAGAAGGAGCATCTCAAGATGCAAAAGCTGTGAAGGACAATATTTTGAATCAAATCACAAAAGCTGTGCAAATGGTGAAGCTGGAGAAAGATCCACACGCGGCATTTGCTTTGATCATTGACGGGAAAACCCTAACGTATGCATTGGAGGATGAGATGAAGTATCAGTTTCTTGCTTTGGCGGTCGATTGTGCCTCAGTCATTTGCTGTCGTGTGTCTCCAAAGCAGAAAGCTTTGGTAACGAGGTTAGTTAAAGAGGGAACCGGGAAAATCACATTGGCAATAGGTGATGGTGCGAATGATGTTGGAATGATTCAAGAAGCTGACATTGGTGTTGGAATTAGTGGGGTTGAAGGAATGCAGGCTGTAATGGCAAGTGATTTTTCTATCGCCCAGTTCCGGTTTCTTGAAAGATTGCTCGTCGTTCATGGACACTGGTGCTACAAAAGAATAGCTCAAATGATCTGCTATTTCTTTTACAAGAACATAGCATTTGGTCTCACTCTCTTCTATTTTGAGGCTTTCACCGGGTTTTCTGGGCAATCAGTATATAATGACTACTACCTATTACTCTTCAACGTCGTCCTTACTTCATTGCCAGTGATTGCCCTTGGAGTCTTTGAACAAGATGTTTCCTCTGAGATCTGTTTACAGTTTCCAGCGTTGTACCAACAAGGGAAGAAAAATCTCTTCTTTGACTGGTATAGAATACTTGGGTGGATGGGAAATGGTGTGTACTCCTCCCTTGTAATATTCTTCCTCAACATTGGAATCATTTATGAGCAAGCTTTCAGAGTCAGTGGCCAAACTGCTGACATGGATGCTGTTGGTACTACCATGTTTACCTGCATTATTTGGGCTGTGAATGTGCAAATCGCATTGACGGTGAGTCACTTCACTTGGATCCAACACGTCTTGATCTGGGGAAGCATTGGTTTATGGTATCTGTTTGTTGCGCTCTACGGGATGATGCCTCCAAGTCTTTCAGGCAACATCTATAGAATTCTAGTAGAGATACTTGCTCCTGCACCCATTTATTGGATAGCCACATTTTTGGTCACAGTGACTACAGTTCTCCCTTACTTTGCCCACATATCATTCCAAAGGTTCTTACATCCGTTGGATCATCACATCATTCAAGAGATAAAATACTACAAGAGAGATGTGGAGGATAGAAGAATGTGGACACGAGAGCGTACAAAAGCACGGGAGAAGACTAAGATTGGATTCACAGCTAGAGTTGACGCGAAAATCAGACACCTACGATCAAAGCTCAATAAGAAACAGTCAAATATGTCACAATTTTCAACTCAAGATACAATGTCCCCAAGATCAGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005488 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006950 GO:0006996 GO:0008150 GO:0008270 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010286 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016049 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019216 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0033036 GO:0034204 GO:0040007 GO:0042623 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045332 GO:0046872 GO:0046914 GO:0048193 GO:0048194 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0060560 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0080090 GO:0097035 GO:0098791 GO:1905038

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1216

Amino Acids

138.19

Weight (kDa)

7.53

Isoelectric Point (pI)

39.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	43 - 110	1.2e-23	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	526 - 720	8.5e-18	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	893 - 1143	6.1e-89	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	3164
AccB1I	GGYRCC	1 cut(s)	2164
AccB7I	CCANNNNNTGG	1 cut(s)	3348
AccI	GTMKAC	1 cut(s)	1923
AccII	CGCG	2 cut(s)	2411, 3557
AciI	CCGC	2 cut(s)	2411, 2492
AclWI	GGATC	8 cut(s)	83, 689, 755, 768, 2396, 3208, 3221, 3441
AcoI	YGGCCR	1 cut(s)	3112
AcsI	RAATTY	4 cut(s)	586, 1485, 1792, 3312
AfiI	CCNNNNNNNGG	5 cut(s)	104, 1496, 2161, 2709, 3348
AflII	CTTAAG	1 cut(s)	1012
AflIII	ACRYGT	2 cut(s)	1185, 3220
AhdI	GACNNNNNGTC	1 cut(s)	3198
AjiI	CACGTC	1 cut(s)	3223
AjnI	CCWGG	1 cut(s)	109
Alw26I	GTCTC	8 cut(s)	25, 572, 593, 974, 1135, 1619, 2532, 2813
AlwI	GGATC	8 cut(s)	83, 689, 755, 768, 2396, 3208, 3221, 3441
AlwNI	CAGNNNCTG	1 cut(s)	775
Ama87I	CYCGRG	2 cut(s)	1449, 1610
AoxI	GGCC	4 cut(s)	65, 2046, 2197, 3112
ApeKI	GCWGC	6 cut(s)	254, 278, 1093, 1370, 1373, 1682
ApoI	RAATTY	4 cut(s)	586, 1485, 1792, 3312
Asp700I	GAANNNNTTC	1 cut(s)	243
AspLEI	GCGC	2 cut(s)	744, 3268
AspS9I	GGNCC	3 cut(s)	107, 621, 1931
AsuC2I	CCSGG	2 cut(s)	2575, 2839
AvaI	CYCGRG	2 cut(s)	1449, 1610
AvaII	GGWCC	3 cut(s)	107, 621, 1931
AxyI	CCTNAGG	1 cut(s)	1020
BaeGI	GKGCMC	1 cut(s)	2169
BaeI	ACNNNNGTAYC	4 cut(s)	834, 867, 2245, 2278
BalI	TGGCCA	1 cut(s)	3114
BamHI	GGATCC	2 cut(s)	760, 3213
BanI	GGYRCC	1 cut(s)	2164
BanII	GRGCYC	2 cut(s)	357, 1444
BauI	CACGAG	2 cut(s)	2257, 3498
BbsI	GAAGAC	1 cut(s)	3530
BbvI	GCAGC	6 cut(s)	241, 265, 1080, 1357, 1360, 1669
BceAI	ACGGC	1 cut(s)	529
BcgI	CGANNNNNNTGC	4 cut(s)	1209, 1243, 1352, 1386
BciT130I	CCWGG	1 cut(s)	111
BciVI	GTATCC	3 cut(s)	1252, 1426, 2246
BclI	TGATCA	3 cut(s)	210, 1080, 2424
BcnI	CCSGG	2 cut(s)	2575, 2839
BcoDI	GTCTC	8 cut(s)	25, 572, 593, 974, 1135, 1619, 2532, 2813
BfaI	CTAG	6 cut(s)	198, 1247, 1686, 1809, 3317, 3546
BfmI	CTRYAG	3 cut(s)	2058, 3307, 3378
BfoI	RGCGCY	1 cut(s)	745
BfrI	CTTAAG	1 cut(s)	1012
BfuAI	ACCTGC	1 cut(s)	3164
BfuI	GTATCC	3 cut(s)	1252, 1426, 2246
BglII	AGATCT	2 cut(s)	1177, 2952
BisI	GCNGC	7 cut(s)	255, 279, 1094, 1371, 1374, 1683, 2412
BlsI	GCNGC	7 cut(s)	256, 280, 1095, 1372, 1375, 1684, 2413
BmcAI	AGTACT	1 cut(s)	1432
Bme1390I	CCNGG	3 cut(s)	111, 2575, 2839
Bme18I	GGWCC	3 cut(s)	107, 621, 1931
BmeRI	GACNNNNNGTC	1 cut(s)	3198
BmeT110I	CYCGRG	2 cut(s)	1449, 1610
BmgBI	CACGTC	1 cut(s)	3223
BmgT120I	GGNCC	3 cut(s)	107, 621, 1931
BmiI	GGNNCC	7 cut(s)	762, 1026, 1209, 1932, 2166, 2572, 3215
BmrFI	CCNGG	3 cut(s)	111, 2575, 2839
BmrI	ACTGGG	2 cut(s)	2229, 2698
BmsI	GCATC	7 cut(s)	677, 843, 1169, 2320, 2330, 3121, 3270
BmuI	ACTGGG	2 cut(s)	2229, 2698
BpiI	GAAGAC	1 cut(s)	3530
BpmI	CTGGAG	2 cut(s)	2138, 2414
Bpu10I	CCTNAGC	2 cut(s)	794, 1443
BpuEI	CTTGAG	4 cut(s)	752, 2105, 2310, 3606
BpuMI	CCSGG	2 cut(s)	2575, 2839
BsaAI	YACGTR	1 cut(s)	1841
BsaBI	GATNNNNATC	1 cut(s)	1115
BsaI	GGTCTC	1 cut(s)	2813
BsaJI	CCNNGG	4 cut(s)	103, 624, 1436, 2920
BsaWI	WCCGGW	1 cut(s)	2709
BsaXI	ACNNNNNCTCC	8 cut(s)	480, 510, 810, 840, 971, 1001, 1417, 1447
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 1496, 2161, 2709, 3348
Bse21I	CCTNAGG	1 cut(s)	1020
Bse3DI	GCAATG	1 cut(s)	2903
Bse8I	GATNNNNATC	1 cut(s)	1115
BseBI	CCWGG	1 cut(s)	111
BseDI	CCNNGG	4 cut(s)	103, 624, 1436, 2920
BseGI	GGATG	9 cut(s)	62, 565, 652, 1534, 2467, 3036, 3136, 3282, 3424
BseJI	GATNNNNATC	1 cut(s)	1115
BseLI	CCNNNNNNNGG	5 cut(s)	104, 1496, 2161, 2709, 3348
BseMI	GCAATG	1 cut(s)	2903
BseRI	GAGGAG	3 cut(s)	1037, 1637, 3040
BseSI	GKGCMC	1 cut(s)	2169
BseXI	GCAGC	6 cut(s)	241, 265, 1080, 1357, 1360, 1669
BseYI	CCCAGC	1 cut(s)	704
BsgI	GTGCAG	3 cut(s)	93, 1359, 3321
Bsh1236I	CGCG	2 cut(s)	2411, 3557
Bsh1285I	CGRYCG	1 cut(s)	2496
BshFI	GGCC	4 cut(s)	67, 2048, 2199, 3114
BshNI	GGYRCC	1 cut(s)	2164
BsiEI	CGRYCG	1 cut(s)	2496
BsiHKCI	CYCGRG	2 cut(s)	1449, 1610
BsiSI	CCGG	3 cut(s)	2574, 2710, 2838
BslFI	GGGAC	2 cut(s)	1944, 3619
BslI	CCNNNNNNNGG	5 cut(s)	104, 1496, 2161, 2709, 3348
BsmAI	GTCTC	8 cut(s)	25, 572, 593, 974, 1135, 1619, 2532, 2813
BsmFI	GGGAC	2 cut(s)	1944, 3619
BsmI	GAATGC	1 cut(s)	2673
BsnI	GGCC	4 cut(s)	67, 2048, 2199, 3114
Bso31I	GGTCTC	1 cut(s)	2813
BsoBI	CYCGRG	2 cut(s)	1449, 1610
Bsp1286I	GDGCHC	3 cut(s)	357, 1444, 2169
BspACI	CCGC	2 cut(s)	2411, 2492
BspANI	GGCC	4 cut(s)	67, 2048, 2199, 3114
BspFNI	CGCG	2 cut(s)	2411, 3557
BspHI	TCATGA	1 cut(s)	1738
BspLI	GGNNCC	7 cut(s)	762, 1026, 1209, 1932, 2166, 2572, 3215
BspMI	ACCTGC	1 cut(s)	3164
BspPI	GGATC	8 cut(s)	83, 689, 755, 768, 2396, 3208, 3221, 3441
BspQI	GCTCTTC	1 cut(s)	1236
BspT107I	GGYRCC	1 cut(s)	2164
BspTI	CTTAAG	1 cut(s)	1012
BspTNI	GGTCTC	1 cut(s)	2813
BsrDI	GCAATG	1 cut(s)	2903
BssECI	CCNNGG	4 cut(s)	103, 624, 1436, 2920
BssNAI	GTATAC	1 cut(s)	1924
BssSI	CACGAG	2 cut(s)	2257, 3498
BssT1I	CCWWGG	4 cut(s)	103, 624, 1436, 2920
Bst1107I	GTATAC	1 cut(s)	1924
Bst2BI	CACGAG	2 cut(s)	2257, 3498
Bst2UI	CCWGG	1 cut(s)	111
Bst6I	CTCTTC	4 cut(s)	1236, 2822, 2888, 3005
BstAFI	CTTAAG	1 cut(s)	1012
BstBAI	YACGTR	1 cut(s)	1841
BstC8I	GCNNGC	6 cut(s)	1158, 1222, 1330, 2197, 2673, 3096
BstF5I	GGATG	9 cut(s)	62, 565, 652, 1534, 2467, 3036, 3136, 3282, 3424
BstFNI	CGCG	2 cut(s)	2411, 3557
BstH2I	RGCGCY	1 cut(s)	745
BstHHI	GCGC	2 cut(s)	744, 3268
BstMAI	GTCTC	8 cut(s)	25, 572, 593, 974, 1135, 1619, 2532, 2813
BstMCI	CGRYCG	1 cut(s)	2496
BstMWI	GCNNNNNNNGC	4 cut(s)	148, 2030, 2417, 2681
BstNI	CCWGG	1 cut(s)	111
BstNSI	RCATGY	2 cut(s)	997, 1189
BstSCI	CCNGG	3 cut(s)	109, 2573, 2837
BstSFI	CTRYAG	3 cut(s)	2058, 3307, 3378
BstSLI	GKGCMC	1 cut(s)	2169
BstUI	CGCG	2 cut(s)	2411, 3557
BstV1I	GCAGC	6 cut(s)	241, 265, 1080, 1357, 1360, 1669
BstV2I	GAAGAC	1 cut(s)	3530
BstX2I	RGATCY	7 cut(s)	88, 694, 760, 1177, 2401, 2952, 3213
BstXI	CCANNNNNNTGG	1 cut(s)	3364
BstYI	RGATCY	7 cut(s)	88, 694, 760, 1177, 2401, 2952, 3213
BstZ17I	GTATAC	1 cut(s)	1924
Bsu36I	CCTNAGG	1 cut(s)	1020
BsuI	GTATCC	3 cut(s)	1252, 1426, 2246
BsuRI	GGCC	4 cut(s)	67, 2048, 2199, 3114
BtgZI	GCGATG	1 cut(s)	459
BtrI	CACGTC	1 cut(s)	3223
BtsCI	GGATG	9 cut(s)	62, 565, 652, 1534, 2467, 3036, 3136, 3282, 3424
BtsI	GCAGTG	1 cut(s)	1083
BtsIMutI	CAGTG	7 cut(s)	335, 755, 1083, 2743, 2916, 3115, 3378
BveI	ACCTGC	1 cut(s)	3164
Cac8I	GCNNGC	6 cut(s)	1158, 1222, 1330, 2197, 2673, 3096
CaiI	CAGNNNCTG	1 cut(s)	775
CciI	TCATGA	1 cut(s)	1738
CfoI	GCGC	2 cut(s)	744, 3268
Cfr13I	GGNCC	3 cut(s)	107, 621, 1931
CseI	GACGC	1 cut(s)	3563
CspCI	CAANNNNNGTGG	2 cut(s)	2121, 2156
CviAII	CATG	9 cut(s)	839, 994, 1186, 1406, 1739, 2281, 2738, 3127, 3148
DraI	TTTAAA	1 cut(s)	1950
DriI	GACNNNNNGTC	1 cut(s)	3198
EaeI	YGGCCR	1 cut(s)	3112
Eam1104I	CTCTTC	4 cut(s)	1236, 2822, 2888, 3005
Eam1105I	GACNNNNNGTC	1 cut(s)	3198
EarI	CTCTTC	4 cut(s)	1236, 2822, 2888, 3005
Eco130I	CCWWGG	4 cut(s)	103, 624, 1436, 2920
Eco24I	GRGCYC	2 cut(s)	357, 1444
Eco31I	GGTCTC	1 cut(s)	2813
Eco32I	GATATC	1 cut(s)	2094
Eco47I	GGWCC	3 cut(s)	107, 621, 1931
Eco81I	CCTNAGG	1 cut(s)	1020
Eco88I	CYCGRG	2 cut(s)	1449, 1610
EcoO109I	RGGNCCY	2 cut(s)	621, 1931
EcoRI	GAATTC	2 cut(s)	1485, 3312
EcoRII	CCWGG	1 cut(s)	109
EcoRV	GATATC	1 cut(s)	2094
EcoT14I	CCWWGG	4 cut(s)	103, 624, 1436, 2920
EcoT22I	ATGCAT	3 cut(s)	2177, 2297, 2455
EcoT38I	GRGCYC	2 cut(s)	357, 1444
ErhI	CCWWGG	4 cut(s)	103, 624, 1436, 2920
FaeI	CATG	9 cut(s)	842, 997, 1189, 1409, 1742, 2284, 2741, 3130, 3151
FaqI	GGGAC	2 cut(s)	1944, 3619
FatI	CATG	9 cut(s)	838, 993, 1185, 1405, 1738, 2280, 2737, 3126, 3147
FauNDI	CATATG	1 cut(s)	147
FbaI	TGATCA	3 cut(s)	210, 1080, 2424
FblI	GTMKAC	1 cut(s)	1923
Fnu4HI	GCNGC	7 cut(s)	255, 279, 1094, 1371, 1374, 1683, 2412
FokI	GGATG	9 cut(s)	69, 572, 659, 1541, 2474, 3043, 3143, 3289, 3411
FriOI	GRGCYC	2 cut(s)	357, 1444
Fsp4HI	GCNGC	7 cut(s)	255, 279, 1094, 1371, 1374, 1683, 2412
FspBI	CTAG	6 cut(s)	198, 1247, 1686, 1809, 3317, 3546
GlaI	GCGC	2 cut(s)	743, 3267
GluI	GCNGC	7 cut(s)	255, 279, 1094, 1371, 1374, 1683, 2412
GsaI	CCCAGC	1 cut(s)	708
GsuI	CTGGAG	2 cut(s)	2138, 2414
HaeII	RGCGCY	1 cut(s)	745
HaeIII	GGCC	4 cut(s)	67, 2048, 2199, 3114
HapII	CCGG	3 cut(s)	2574, 2710, 2838
HgaI	GACGC	1 cut(s)	3563
HhaI	GCGC	2 cut(s)	744, 3268
Hin1II	CATG	9 cut(s)	842, 997, 1189, 1409, 1742, 2284, 2741, 3130, 3151
Hin6I	GCGC	2 cut(s)	742, 3266
HinP1I	GCGC	2 cut(s)	742, 3266
HincII	GTYRAC	1 cut(s)	3553
HindII	GTYRAC	1 cut(s)	3553
HindIII	AAGCTT	5 cut(s)	791, 1665, 2000, 2541, 3096
HpaII	CCGG	3 cut(s)	2574, 2710, 2838
Hpy99I	CGWCG	3 cut(s)	462, 2735, 2894
HpyAV	CCTTC	7 cut(s)	74, 226, 885, 1844, 2309, 2338, 2657
HpyCH4IV	ACGT	5 cut(s)	1348, 1840, 2447, 2889, 3222
HpyF10VI	GCNNNNNNNGC	4 cut(s)	148, 2030, 2417, 2681
HpySE526I	ACGT	5 cut(s)	1348, 1840, 2447, 2889, 3222
Hsp92II	CATG	9 cut(s)	842, 997, 1189, 1409, 1742, 2284, 2741, 3130, 3151
HspAI	GCGC	2 cut(s)	742, 3266
Ksp22I	TGATCA	3 cut(s)	210, 1080, 2424
LguI	GCTCTTC	1 cut(s)	1236
LmnI	GCTCC	6 cut(s)	337, 885, 1024, 1877, 2318, 3337
Lsp1109I	GCAGC	6 cut(s)	241, 265, 1080, 1357, 1360, 1669
LweI	GCATC	7 cut(s)	677, 843, 1169, 2320, 2330, 3121, 3270
MaeI	CTAG	6 cut(s)	198, 1247, 1686, 1809, 3317, 3546
MaeII	ACGT	5 cut(s)	1348, 1840, 2447, 2889, 3222
MfeI	CAATTG	1 cut(s)	1323
MflI	RGATCY	7 cut(s)	88, 694, 760, 1177, 2401, 2952, 3213
MhlI	GDGCHC	3 cut(s)	357, 1444, 2169
MlsI	TGGCCA	1 cut(s)	3114
MluNI	TGGCCA	1 cut(s)	3114
MlyI	GAGTC	6 cut(s)	650, 1661, 1965, 2934, 3114, 3208
MmeI	TCCRAC	7 cut(s)	466, 624, 1779, 2596, 2626, 3241, 3411
Mox20I	TGGCCA	1 cut(s)	3114
Mph1103I	ATGCAT	3 cut(s)	2177, 2297, 2455
MroXI	GAANNNNTTC	1 cut(s)	243
MscI	TGGCCA	1 cut(s)	3114
MseI	TTAA	7 cut(s)	365, 1013, 1037, 1617, 1949, 2216, 2562
MslI	CAYNNNNRTG	5 cut(s)	55, 1188, 1557, 1950, 2300
Msp20I	TGGCCA	1 cut(s)	3114
MspCI	CTTAAG	1 cut(s)	1012
MspI	CCGG	3 cut(s)	2574, 2710, 2838
MspR9I	CCNGG	3 cut(s)	111, 2575, 2839
MunI	CAATTG	1 cut(s)	1323
Mva1269I	GAATGC	1 cut(s)	2673
MvaI	CCWGG	1 cut(s)	111
MvnI	CGCG	2 cut(s)	2411, 3557
MwoI	GCNNNNNNNGC	4 cut(s)	148, 2030, 2417, 2681
NciI	CCSGG	2 cut(s)	2575, 2839
NdeI	CATATG	1 cut(s)	147
NlaIII	CATG	9 cut(s)	842, 997, 1189, 1409, 1742, 2284, 2741, 3130, 3151
NlaIV	GGNNCC	7 cut(s)	762, 1026, 1209, 1932, 2166, 2572, 3215
NmuCI	GTSAC	7 cut(s)	1595, 1653, 1841, 3200, 3367, 3373, 3606
NsiI	ATGCAT	3 cut(s)	2177, 2297, 2455
NspI	RCATGY	2 cut(s)	997, 1189
PaeR7I	CTCGAG	1 cut(s)	1449
PagI	TCATGA	1 cut(s)	1738
PciI	ACATGT	1 cut(s)	1185
PciSI	GCTCTTC	1 cut(s)	1236
PctI	GAATGC	1 cut(s)	2673
PdmI	GAANNNNTTC	1 cut(s)	243
PfeI	GAWTC	9 cut(s)	772, 785, 891, 1231, 1858, 2359, 2623, 3081, 3537
PflMI	CCANNNNNTGG	1 cut(s)	3348
PfoI	TCCNGGA	1 cut(s)	109
PkrI	GCNGC	7 cut(s)	256, 280, 1095, 1372, 1375, 1684, 2413
PleI	GAGTC	6 cut(s)	650, 1660, 1965, 2933, 3113, 3207
PpsI	GAGTC	6 cut(s)	650, 1660, 1965, 2933, 3113, 3207
Ppu21I	YACGTR	1 cut(s)	1841
PpuMI	RGGWCCY	2 cut(s)	621, 1931
PscI	ACATGT	1 cut(s)	1185
Psp5II	RGGWCCY	2 cut(s)	621, 1931
Psp6I	CCWGG	1 cut(s)	109
PspFI	CCCAGC	1 cut(s)	704
PspGI	CCWGG	1 cut(s)	109
PspN4I	GGNNCC	7 cut(s)	762, 1026, 1209, 1932, 2166, 2572, 3215
PspPI	GGNCC	3 cut(s)	107, 621, 1931
PspPPI	RGGWCCY	2 cut(s)	621, 1931
PstNI	CAGNNNCTG	1 cut(s)	775
PsuI	RGATCY	7 cut(s)	88, 694, 760, 1177, 2401, 2952, 3213
RseI	CAYNNNNRTG	5 cut(s)	55, 1188, 1557, 1950, 2300
SapI	GCTCTTC	1 cut(s)	1236
SaqAI	TTAA	7 cut(s)	365, 1013, 1037, 1617, 1949, 2216, 2562
SatI	GCNGC	7 cut(s)	255, 279, 1094, 1371, 1374, 1683, 2412
Sau96I	GGNCC	3 cut(s)	107, 621, 1931
ScaI	AGTACT	1 cut(s)	1432
SchI	GAGTC	6 cut(s)	650, 1661, 1965, 2934, 3114, 3208
ScrFI	CCNGG	3 cut(s)	111, 2575, 2839
SduI	GDGCHC	3 cut(s)	357, 1444, 2169
SfaNI	GCATC	7 cut(s)	677, 843, 1169, 2320, 2330, 3121, 3270
SfcI	CTRYAG	3 cut(s)	2058, 3307, 3378
Sfr274I	CTCGAG	1 cut(s)	1449
SinI	GGWCC	3 cut(s)	107, 621, 1931
SlaI	CTCGAG	1 cut(s)	1449
SmiI	ATTTAAAT	1 cut(s)	1950
SmiMI	CAYNNNNRTG	5 cut(s)	55, 1188, 1557, 1950, 2300
SmlI	CTYRAG	6 cut(s)	731, 1012, 1449, 2084, 2325, 3621
SmoI	CTYRAG	6 cut(s)	731, 1012, 1449, 2084, 2325, 3621
SsiI	CCGC	2 cut(s)	2411, 2492
SspI	AATATT	3 cut(s)	2018, 2353, 3063
SspMI	CTAG	6 cut(s)	198, 1247, 1686, 1809, 3317, 3546
StyD4I	CCNGG	3 cut(s)	109, 2573, 2837
StyI	CCWWGG	4 cut(s)	103, 624, 1436, 2920
SwaI	ATTTAAAT	1 cut(s)	1950
TaiI	ACGT	5 cut(s)	1351, 1843, 2450, 2892, 3225
TaqI	TCGA	7 cut(s)	894, 1229, 1362, 1450, 1509, 2106, 2496
TatI	WGTACW	4 cut(s)	129, 1430, 1635, 3045
TauI	GCSGC	1 cut(s)	2414
TfiI	GAWTC	9 cut(s)	772, 785, 891, 1231, 1858, 2359, 2623, 3081, 3537
Tru1I	TTAA	7 cut(s)	365, 1013, 1037, 1617, 1949, 2216, 2562
Tru9I	TTAA	7 cut(s)	365, 1013, 1037, 1617, 1949, 2216, 2562
TscAI	CASTG	7 cut(s)	342, 762, 1090, 2750, 2916, 3115, 3378
TseFI	GTSAC	7 cut(s)	1595, 1653, 1841, 3200, 3367, 3373, 3606
TseI	GCWGC	6 cut(s)	254, 278, 1093, 1370, 1373, 1682
Tsp45I	GTSAC	7 cut(s)	1595, 1653, 1841, 3200, 3367, 3373, 3606
TspGWI	ACGGA	4 cut(s)	280, 1116, 1964, 3417
TspRI	CASTG	7 cut(s)	342, 762, 1090, 2750, 2916, 3115, 3378
Van91I	CCANNNNNTGG	1 cut(s)	3348
Vha464I	CTTAAG	1 cut(s)	1012
VpaK11BI	GGWCC	3 cut(s)	107, 621, 1931
XapI	RAATTY	4 cut(s)	586, 1485, 1792, 3312
XceI	RCATGY	2 cut(s)	997, 1189
XhoI	CTCGAG	1 cut(s)	1449
XmiI	GTMKAC	1 cut(s)	1923
XmnI	GAANNNNTTC	1 cut(s)	243
XspI	CTAG	6 cut(s)	198, 1247, 1686, 1809, 3317, 3546
ZrmI	AGTACT	1 cut(s)	1432
Zsp2I	ATGCAT	3 cut(s)	2177, 2297, 2455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.