Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Reverse (-)

16623318 .. 16629632

Gene Structure 6315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1190200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3684 bp

ATGGCAGGAGGTAAGATGAGGGCGAAGCTTCGCCAGAGCAACCTTTACACATTCTGCCGTAAGCCTAAAGCCGATGAGACAGATGCGCCTCGCCCAATCCAAGGAGCTGGGTTCACAAGAACTGTATATTGCAACCAACCTCATTTCCATCAAAAGAAGCCTTATAAATACCGTTCAAATTATATATCAACCACCAAGTATAACGTTATCACGTTCTTGCCCAAAGCACTCTTTGAGCAGTTCAGACGGGTTGCCAATGTGTATTTCCTTTTAGCTGCAATTCTTTCCCTCACACCTGTTTCACCTTTCTCTCCAGTGAGTATGATTGCTCCTCTGGTATTTGTTGTTGGTCTTAGTATGGCAAAGGAAGCGATGGAAGATTGGAATAGGTTCTTGCAGGATATGAAGGTTAATCGACGTAAAGTTATCGTTCATAAAGGAGATGGTGTTTTTGATTTTAGGCCATGGCATAAGATTCGGGTTGGAGATGTTGTGAAAGTGGAAAAGGATCAATTCTTTCCTGCAGATTTGCTTCTTTTGTCATCAAGTTATGAGGATGGGATTTGTTATGTTGAGACTATGAACTTAGATGGTGAAACAAATTTGAAGGTAAAGAGATGTTTGGAAGTAACCTCGCCAATGGAGGATGATGGCGCTTTCAAGGATTTGACGGCAACAATTCAATGTGAAGATCCAAACCCCAATCTTTACTCATTCGTAGGTAATCTTGAGTATGATCGGCAGGTGTATCCTCTTGAACCCAATCAGATACTCTTGAGAGATTCGAAGCTCAGGAATACAGCTTATGTGTATGGGGTGGTGATATTTACAGGTCATGATAGCAAAGTCATGCAGAATTCAACGAAGTCCCCTTCAAAAAGGAGCGGAATAGAAAGGAAAATGGATCACATTATCTATATTCTTTTCACCCTCCTTTTAGGGATTTCAATGATCAGCTCAATAGGCTTTGCTGTGAAGATAAAATTCAGCATGCCAGACTCGTGGTACTTACGACCTGATAATACGACGGATATGTATAGCCCAAAAAAGCCTGCCTTGTCCGGCCTTATCCATTTGGTCACCGCTCTCATCCTTTATGGATATCTAATACCTATCTCTCTCTATGTTTCAATTGAGGTTGTGAAGGTTTTGCAAGCAAGCTTTATTAACCAAGACTTACTCATGTATGATGAAGAGACTGGGAAGCCTGCTGAAGCACGGACATCAAATTTGAATGAGGAGTTAGGCCAGGTAGACACGATCCTCTCTGATAAAACTGGCACATTAACCTGCAACCAGATGGACTTTCTGAAGTGTTCCATTGCTGGTACTGCCTATGGCGTTCGTTCCAGTGAAGTTGAACTTGCAGCAGCAAAACAGATGGCTAGTGACCTTGAAGACCGTGATGATGAGCTTGCCAATTTTCCCATGCGAAAACATAATGCGCATGTATCATGGGAGAATGGAATAGGATCGGAAATTGAGCTAGAGACTGTTGTTACGTCCAATGAAGATAAGGATCGGAAACCTGCCATAAAGGGGTTTAGCTTTGAGGACAGCCGCCTCATGGGAGGAAATTGGTTGAATGAGTCCAGCCCTGATGTGATTTCATTATTTTTACGGATATTAGCAGTTTGCCACACTGCAATTCCAGAGGTAAATGAAGAGACTGGCAGTTATACATATGAAGCCGAGTCACCGGATGAAGCAGCTTTTCTTGTTGCAGCAAGAGAGTTAGGATTTGAGTTTTGTAAAAGAAGTCAATCAGGTGTGTTTGTCCTCGAAAGGTATCATCATTCAGGACGACCAGTGGAAAGGGAGTACAAAATCTTAAATCTATTGGAATTCACTAGCAAAAGGAAGCGTATGTCTGTAATTGTGCGAGATGAAGATGGACAGCTTTTACTGATGTGCAAAGGTGCTGACAGCATAATTTTCGATCGACTTTCAAAGAATGGAAGAATGTATGAGGAAGCTACTACTAAGCATTTGAATGAATATGGAGAAGCTGGGTTGCGTACATTGGCACTTGCTTATAGAAAGCTTGAGGAGTCCGAGTACTCTGCTTGGAGTAATGAGTTTCAGAAAGCAAAAACATCAATTGGGGCTGATAGGGATGGAATGCTTGAGCGACTAGCAGATAAGATGGAAAGAGACTTGATCCTTGTTGGTGCTACAGCTGTAGAGGACAAATTGCAAAAAGGGGTGCCCCAGTGCATAGACAATCTCGCACAAGCTGGTCTCAAGATCTGGGTTTTGACGGGGGACAAGATGGAAACTGCAATCAACATAGGATTTGCATGTAGTTTGCTCCGACAGGGCATGAAGCAGATTTGTATATCAACCACAAATTTAGACACGTTGGGACAAGATAGCAAAGAGGCGGTGAAAGATAACATTTTGAATCAAATCACCAATGCCTCACAAATGATCAAGTTGGAAAAAGATCCACATGCGGCATTTGCATTAATTATTGATGGAAAAACTTTAACCTATGCTTTGGAGGATGATATGAAGCATCTATTCTTGGGATTAGCTGTTGACTGTGCATCAGTCATATGCTGCCGTGTCTCTCCCAAGCAGAAGGCATTGGTAACAAGGTTAGTCAAACAAGGAACTGGAAAAACGACGTTAGCAATTGGCGATGGTGCAAATGATGTTGGAATGATTCAAGAAGCTGATATTGGTGTTGGAATCAGTGGGGTCGAAGGTATGCAGGCTGTGATGGCTAGTGACTTTGCTATTTCCCAGTTTCGGTATTTGGAGAGACTTTTGGTAGTCCACGGCCATTGGTGCTATAAGAGGATTGCTCAGATGGTCTGCTATTTCTTCTACAAAAATATTGCATTTGGTCTCACCCTCTTCTACTTCGAGGCATTCACGGGTTTCTCTGGGCAATCAATATATGATGACTGGTACATGTTATCATTCAATGTCATTCTAACCTCATTGCCTGTCATTTCACTTGGAGTTTTTGAACAAGATGTTTCGTCCGAGGTCTGCTTACAGTTCCCAGCATTGTACCAACAAGGACCTAGAAATCTGTTCTTCGATTGGTACAGGATACTGGGGTGGATGGGCAATGGTGTCTACTGCTCTCTCATAATCTACTTCCTCAACATTATCATCTTCTACGACCAGGCCTTCCGTGCTAATGGCCAGACGGCTGATATGGCTGCTATGGGTACCACTATGTTCAGTTGCATCGTGTGGGCTGTGAACTGCCAGATTGCTCTCACAATGAGCCACTTTACGTGGATTCAGCATCTCTTTGTGTGGGGAAGTATTGCAATGTGGTACCTATTTCTCTTAATTTACGGCATGCTGACTCCAACTTGGAGTAAAAATGCCTACCAAATTCTAGTCGAAGCTCTCGGTCCTGCCCCATTATTTTGGTCAGCCACCCTCTTAGTAACAATCGCTTGTAATCTCCCTTACATTGTCCACTTAGCCTTCCAAAGAAGTTTCAATCCAATGGACCATCATATCATCCAAGAAATCAAATACTACAAGAAGGATGTCGAGGATCAGCGCATGTGGAAGAGGGAGGCGTCGAAAGCAAGACAAGAAACCAAGATAGGGTTCACGGCGAGAGTGGATGCCAAGATCAGACACTTGAGAGGGAAGCTACATAAGAAGCATCATACTCCTGTAAGTACGCAAGGAGGGATGTCACCATCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1228

Amino Acids

138.7

Weight (kDa)

6.63

Isoelectric Point (pI)

38.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	42 - 109	6.4e-26	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	539 - 734	2.1e-22	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	908 - 1158	5.4e-90	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	165
AarI	CACCTGC	1 cut(s)	733
Acc16I	TGCGCA	1 cut(s)	1446
Acc36I	ACCTGC	3 cut(s)	733, 1298, 1537
Acc65I	GGTACC	2 cut(s)	3185, 3297
AccB1I	GGYRCC	3 cut(s)	2206, 3185, 3297
AccBSI	CCGCTC	2 cut(s)	885, 1085
AccI	GTMKAC	2 cut(s)	1254, 3090
AciI	CCGC	5 cut(s)	885, 1083, 1561, 2384, 2456
AclI	AACGTT	1 cut(s)	204
AclWI	GGATC	9 cut(s)	516, 686, 912, 1255, 1480, 1527, 2155, 2441, 3534
AcoI	YGGCCR	2 cut(s)	2785, 3157
AcsI	RAATTY	7 cut(s)	601, 856, 983, 1228, 1844, 2350, 3357
AcuI	CTGAAG	2 cut(s)	1233, 1331
AcyI	GRCGYC	1 cut(s)	3551
AfiI	CCNNNNNNNGG	5 cut(s)	101, 1539, 1567, 2754, 3579
AflIII	ACRYGT	2 cut(s)	2358, 2919
AjnI	CCWGG	2 cut(s)	1248, 3138
AjuI	GAANNNNNNNTTGG	6 cut(s)	248, 280, 2667, 2699, 3566, 3598
AleI	CACNNNNGTG	1 cut(s)	3679
AlwI	GGATC	9 cut(s)	516, 686, 912, 1255, 1480, 1527, 2155, 2441, 3534
AoxI	GGCC	6 cut(s)	461, 1063, 1246, 2785, 3141, 3157
ApeKI	GCWGC	7 cut(s)	275, 1367, 1370, 1709, 1724, 2562, 3176
ApoI	RAATTY	7 cut(s)	601, 856, 983, 1228, 1844, 2350, 3357
ArsI	GACNNNNNNTTYG	2 cut(s)	1213, 1245
AseI	ATTAAT	1 cut(s)	2468
Asp700I	GAANNNNTTC	1 cut(s)	389
Asp718I	GGTACC	2 cut(s)	3185, 3297
AspLEI	GCGC	4 cut(s)	88, 656, 1447, 3534
AspS9I	GGNCC	3 cut(s)	3032, 3377, 3478
AsuII	TTCGAA	1 cut(s)	785
AvaII	GGWCC	3 cut(s)	3032, 3377, 3478
BaeGI	GKGCMC	1 cut(s)	2211
BaeI	ACNNNNGTAYC	2 cut(s)	3056, 3089
BalI	TGGCCA	1 cut(s)	3159
BanI	GGYRCC	3 cut(s)	2206, 3185, 3297
BauI	CACGAG	1 cut(s)	1000
BbsI	GAAGAC	1 cut(s)	1404
BbvI	GCAGC	7 cut(s)	262, 1379, 1382, 1721, 1736, 2549, 3163
BceAI	ACGGC	7 cut(s)	42, 687, 2550, 2800, 3180, 3334, 3603
BcgI	CGANNNNNNTGC	6 cut(s)	458, 492, 1918, 1952, 2045, 2079
BciT130I	CCWGG	2 cut(s)	1250, 3140
BciVI	GTATCC	2 cut(s)	759, 3057
BclI	TGATCA	2 cut(s)	951, 2430
BfaI	CTAG	7 cut(s)	1386, 1487, 1851, 2135, 2730, 3036, 3362
BfmI	CTRYAG	3 cut(s)	522, 2175, 2181
BfoI	RGCGCY	1 cut(s)	657
BfuAI	ACCTGC	3 cut(s)	733, 1298, 1537
BfuI	GTATCC	2 cut(s)	759, 3057
BglII	AGATCT	1 cut(s)	2247
BisI	GCNGC	9 cut(s)	276, 1368, 1371, 1561, 1710, 1725, 2457, 2563, 3177
BlsI	GCNGC	9 cut(s)	277, 1369, 1372, 1562, 1711, 1726, 2458, 2564, 3178
BmcAI	AGTACT	1 cut(s)	2060
Bme1390I	CCNGG	2 cut(s)	1250, 3140
Bme18I	GGWCC	3 cut(s)	3032, 3377, 3478
BmgT120I	GGNCC	3 cut(s)	3032, 3377, 3478
BmiI	GGNNCC	3 cut(s)	2208, 3187, 3299
BmrFI	CCNGG	2 cut(s)	1250, 3140
BmrI	ACTGGG	4 cut(s)	1209, 2206, 2743, 3077
BmsI	GCATC	7 cut(s)	73, 2527, 2558, 3213, 3274, 3589, 3649
BmuI	ACTGGG	4 cut(s)	1209, 2206, 2743, 3077
BpiI	GAAGAC	1 cut(s)	1404
BplI	GAGNNNNNCTC	2 cut(s)	2794, 2826
BpmI	CTGGAG	1 cut(s)	297
Bpu10I	CCTNAGC	1 cut(s)	791
Bpu14I	TTCGAA	1 cut(s)	785
BpuEI	CTTGAG	6 cut(s)	749, 796, 2066, 2147, 2228, 3637
BsaAI	YACGTR	1 cut(s)	3255
BsaBI	GATNNNNATC	1 cut(s)	1518
BsaHI	GRCGYC	1 cut(s)	3551
BsaI	GGTCTC	2 cut(s)	2246, 2858
BsaJI	CCNNGG	4 cut(s)	100, 464, 2782, 2994
BsaWI	WCCGGW	1 cut(s)	1699
BsaXI	ACNNNNNCTCC	6 cut(s)	432, 462, 477, 507, 2042, 2072
Bsc4I	CCNNNNNNNGG	5 cut(s)	101, 1539, 1567, 2754, 3579
Bse3DI	GCAATG	4 cut(s)	1320, 2948, 3088, 3297
Bse8I	GATNNNNATC	1 cut(s)	1518
BseBI	CCWGG	2 cut(s)	1250, 3140
BseDI	CCNNGG	4 cut(s)	100, 464, 2782, 2994
BseJI	GATNNNNATC	1 cut(s)	1518
BseLI	CCNNNNNNNGG	5 cut(s)	101, 1539, 1567, 2754, 3579
BseMI	GCAATG	4 cut(s)	1320, 2948, 3088, 3297
BseMII	CTCAG	2 cut(s)	805, 2825
BseRI	GAGGAG	3 cut(s)	321, 1253, 2063
BseSI	GKGCMC	1 cut(s)	2211
BseXI	GCAGC	7 cut(s)	262, 1379, 1382, 1721, 1736, 2549, 3163
BseYI	CCCAGC	3 cut(s)	107, 2009, 3013
Bsh1285I	CGRYCG	1 cut(s)	1942
BshFI	GGCC	6 cut(s)	463, 1065, 1248, 2787, 3143, 3159
BshNI	GGYRCC	3 cut(s)	2206, 3185, 3297
BsiEI	CGRYCG	1 cut(s)	1942
BsiSI	CCGG	2 cut(s)	1062, 1700
BslFI	GGGAC	3 cut(s)	853, 2279, 2379
BslI	CCNNNNNNNGG	5 cut(s)	101, 1539, 1567, 2754, 3579
BsmFI	GGGAC	3 cut(s)	853, 2279, 2379
BsmI	GAATGC	2 cut(s)	2127, 2876
BsnI	GGCC	6 cut(s)	463, 1065, 1248, 2787, 3143, 3159
Bso31I	GGTCTC	2 cut(s)	2246, 2858
Bsp119I	TTCGAA	1 cut(s)	785
Bsp1286I	GDGCHC	1 cut(s)	2211
Bsp19I	CCATGG	1 cut(s)	464
BspACI	CCGC	5 cut(s)	885, 1083, 1561, 2384, 2456
BspANI	GGCC	6 cut(s)	463, 1065, 1248, 2787, 3143, 3159
BspCNI	CTCAG	2 cut(s)	804, 2824
BspHI	TCATGA	1 cut(s)	835
BspLI	GGNNCC	3 cut(s)	2208, 3187, 3299
BspMAI	CTGCAG	1 cut(s)	526
BspMI	ACCTGC	3 cut(s)	733, 1298, 1537
BspPI	GGATC	9 cut(s)	516, 686, 912, 1255, 1480, 1527, 2155, 2441, 3534
BspT104I	TTCGAA	1 cut(s)	785
BspT107I	GGYRCC	3 cut(s)	2206, 3185, 3297
BspTNI	GGTCTC	2 cut(s)	2246, 2858
BsrBI	CCGCTC	2 cut(s)	885, 1085
BsrDI	GCAATG	4 cut(s)	1320, 2948, 3088, 3297
BssECI	CCNNGG	4 cut(s)	100, 464, 2782, 2994
BssNI	GRCGYC	1 cut(s)	3551
BssSI	CACGAG	1 cut(s)	1000
BssT1I	CCWWGG	2 cut(s)	100, 464
Bst2BI	CACGAG	1 cut(s)	1000
Bst2UI	CCWGG	2 cut(s)	1250, 3140
Bst4CI	ACNGT	6 cut(s)	124, 173, 1403, 1495, 2546, 3009
Bst6I	CTCTTC	4 cut(s)	1188, 1659, 2867, 3536
BstACI	GRCGYC	1 cut(s)	3551
BstBAI	YACGTR	1 cut(s)	3255
BstBI	TTCGAA	1 cut(s)	785
BstC8I	GCNNGC	8 cut(s)	992, 1053, 1155, 1159, 1209, 1416, 2718, 3323
BstDEI	CTNAG	7 cut(s)	353, 586, 791, 1983, 2811, 3409, 3448
BstDSI	CCRYGG	2 cut(s)	464, 2782
BstEII	GGTNACC	1 cut(s)	1078
BstH2I	RGCGCY	1 cut(s)	657
BstHHI	GCGC	4 cut(s)	88, 656, 1447, 3534
BstMCI	CGRYCG	1 cut(s)	1942
BstMWI	GCNNNNNNNGC	9 cut(s)	368, 963, 1331, 2462, 2726, 2793, 3149, 3173, 3557
BstNI	CCWGG	2 cut(s)	1250, 3140
BstNSI	RCATGY	7 cut(s)	994, 1451, 2304, 2456, 2923, 3325, 3538
BstPI	GGTNACC	1 cut(s)	1078
BstSCI	CCNGG	2 cut(s)	1248, 3138
BstSFI	CTRYAG	3 cut(s)	522, 2175, 2181
BstSLI	GKGCMC	1 cut(s)	2211
BstV1I	GCAGC	7 cut(s)	262, 1379, 1382, 1721, 1736, 2549, 3163
BstV2I	GAAGAC	1 cut(s)	1404
BstX2I	RGATCY	3 cut(s)	691, 2247, 2446
BstXI	CCANNNNNNTGG	3 cut(s)	107, 1002, 3393
BstYI	RGATCY	3 cut(s)	691, 2247, 2446
BsuI	GTATCC	2 cut(s)	759, 3057
BsuRI	GGCC	6 cut(s)	463, 1065, 1248, 2787, 3143, 3159
BtgI	CCRYGG	2 cut(s)	464, 2782
BtgZI	GCGATG	2 cut(s)	386, 2658
BtsI	GCAGTG	1 cut(s)	1641
BtsIMutI	CAGTG	6 cut(s)	321, 1357, 1641, 1815, 2219, 2704
BveI	ACCTGC	3 cut(s)	733, 1298, 1537
Cac8I	GCNNGC	8 cut(s)	992, 1053, 1155, 1159, 1209, 1416, 2718, 3323
CciI	TCATGA	1 cut(s)	835
CfoI	GCGC	4 cut(s)	88, 656, 1447, 3534
Cfr13I	GGNCC	3 cut(s)	3032, 3377, 3478
CseI	GACGC	1 cut(s)	3540
CspCI	CAANNNNNGTGG	2 cut(s)	2771, 2806
DdeI	CTNAG	7 cut(s)	353, 586, 791, 1983, 2811, 3409, 3448
EaeI	YGGCCR	2 cut(s)	2785, 3157
Eam1104I	CTCTTC	4 cut(s)	1188, 1659, 2867, 3536
EarI	CTCTTC	4 cut(s)	1188, 1659, 2867, 3536
Eco130I	CCWWGG	2 cut(s)	100, 464
Eco147I	AGGCCT	1 cut(s)	3143
Eco31I	GGTCTC	2 cut(s)	2246, 2858
Eco32I	GATATC	1 cut(s)	1103
Eco47I	GGWCC	3 cut(s)	3032, 3377, 3478
Eco57I	CTGAAG	2 cut(s)	1233, 1331
Eco91I	GGTNACC	1 cut(s)	1078
EcoO109I	RGGNCCY	1 cut(s)	3032
EcoO65I	GGTNACC	1 cut(s)	1078
EcoRI	GAATTC	2 cut(s)	856, 1844
EcoRII	CCWGG	2 cut(s)	1248, 3138
EcoRV	GATATC	1 cut(s)	1103
EcoT14I	CCWWGG	2 cut(s)	100, 464
ErhI	CCWWGG	2 cut(s)	100, 464
FalI	AAGNNNNNCTT	4 cut(s)	1040, 1072, 1347, 1379
FaqI	GGGAC	3 cut(s)	853, 2279, 2379
FauNDI	CATATG	2 cut(s)	1684, 2558
FbaI	TGATCA	2 cut(s)	951, 2430
FblI	GTMKAC	2 cut(s)	1254, 3090
Fnu4HI	GCNGC	9 cut(s)	276, 1368, 1371, 1561, 1710, 1725, 2457, 2563, 3177
Fsp4HI	GCNGC	9 cut(s)	276, 1368, 1371, 1561, 1710, 1725, 2457, 2563, 3177
FspAI	RTGCGCAY	1 cut(s)	1446
FspBI	CTAG	7 cut(s)	1386, 1487, 1851, 2135, 2730, 3036, 3362
FspI	TGCGCA	1 cut(s)	1446
GlaI	GCGC	4 cut(s)	87, 655, 1446, 3533
GluI	GCNGC	9 cut(s)	276, 1368, 1371, 1561, 1710, 1725, 2457, 2563, 3177
GsaI	CCCAGC	3 cut(s)	111, 2013, 3017
GsuI	CTGGAG	1 cut(s)	297
HaeII	RGCGCY	1 cut(s)	657
HaeIII	GGCC	6 cut(s)	463, 1065, 1248, 2787, 3143, 3159
HapII	CCGG	2 cut(s)	1062, 1700
HgaI	GACGC	1 cut(s)	3540
HhaI	GCGC	4 cut(s)	88, 656, 1447, 3534
Hin1I	GRCGYC	1 cut(s)	3551
Hin6I	GCGC	4 cut(s)	86, 654, 1445, 3532
HinP1I	GCGC	4 cut(s)	86, 654, 1445, 3532
HincII	GTYRAC	1 cut(s)	2542
HindII	GTYRAC	1 cut(s)	2542
HindIII	AAGCTT	3 cut(s)	26, 1159, 2042
HpaII	CCGG	2 cut(s)	1062, 1700
Hpy166II	GTNNAC	8 cut(s)	114, 1255, 2542, 2782, 3091, 3220, 3445, 3585
Hpy8I	GTNNAC	8 cut(s)	114, 1255, 2542, 2782, 3091, 3220, 3445, 3585
Hpy99I	CGWCG	4 cut(s)	420, 1030, 2632, 3556
HpyAV	CCTTC	9 cut(s)	400, 601, 882, 1138, 2578, 2702, 3154, 3463, 3508
HpyCH4III	ACNGT	6 cut(s)	124, 173, 1403, 1495, 2546, 3009
HpyCH4IV	ACGT	7 cut(s)	204, 212, 418, 1502, 2360, 2630, 3254
HpyF10VI	GCNNNNNNNGC	9 cut(s)	368, 963, 1331, 2462, 2726, 2793, 3149, 3173, 3557
HpyF3I	CTNAG	7 cut(s)	353, 586, 791, 1983, 2811, 3409, 3448
HpySE526I	ACGT	7 cut(s)	204, 212, 418, 1502, 2360, 2630, 3254
Hsp92I	GRCGYC	1 cut(s)	3551
HspAI	GCGC	4 cut(s)	86, 654, 1445, 3532
KpnI	GGTACC	2 cut(s)	3189, 3301
Ksp22I	TGATCA	2 cut(s)	951, 2430
LmnI	GCTCC	4 cut(s)	104, 334, 882, 2316
Lsp1109I	GCAGC	7 cut(s)	262, 1379, 1382, 1721, 1736, 2549, 3163
LweI	GCATC	7 cut(s)	73, 2527, 2558, 3213, 3274, 3589, 3649
MaeI	CTAG	7 cut(s)	1386, 1487, 1851, 2135, 2730, 3036, 3362
MaeII	ACGT	7 cut(s)	204, 212, 418, 1502, 2360, 2630, 3254
MaeIII	GTNAC	9 cut(s)	628, 1078, 1388, 1498, 1695, 2593, 2732, 3412, 3672
MbiI	CCGCTC	2 cut(s)	885, 1085
MfeI	CAATTG	3 cut(s)	1131, 2100, 2637
MflI	RGATCY	3 cut(s)	691, 2247, 2446
MhlI	GDGCHC	1 cut(s)	2211
MlsI	TGGCCA	1 cut(s)	3159
MluNI	TGGCCA	1 cut(s)	3159
MlyI	GAGTC	5 cut(s)	992, 1598, 1703, 2060, 3322
MmeI	TCCRAC	6 cut(s)	463, 2338, 2418, 2641, 2671, 3356
Mox20I	TGGCCA	1 cut(s)	3159
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	1 cut(s)	3159
MseI	TTAA	7 cut(s)	411, 1167, 1286, 1832, 2468, 2489, 3311
MslI	CAYNNNNRTG	2 cut(s)	1992, 3679
Msp20I	TGGCCA	1 cut(s)	3159
MspA1I	CMGCKG	1 cut(s)	2180
MspI	CCGG	2 cut(s)	1062, 1700
MspR9I	CCNGG	2 cut(s)	1250, 3140
MunI	CAATTG	3 cut(s)	1131, 2100, 2637
Mva1269I	GAATGC	2 cut(s)	2127, 2876
MvaI	CCWGG	2 cut(s)	1250, 3140
MwoI	GCNNNNNNNGC	9 cut(s)	368, 963, 1331, 2462, 2726, 2793, 3149, 3173, 3557
NcoI	CCATGG	1 cut(s)	464
NdeI	CATATG	2 cut(s)	1684, 2558
NlaIV	GGNNCC	3 cut(s)	2208, 3187, 3299
NmeAIII	GCCGAG	1 cut(s)	1717
NmuCI	GTSAC	5 cut(s)	1078, 1388, 1695, 2732, 3672
NsbI	TGCGCA	1 cut(s)	1446
NspI	RCATGY	7 cut(s)	994, 1451, 2304, 2456, 2923, 3325, 3538
NspV	TTCGAA	1 cut(s)	785
OliI	CACNNNNGTG	1 cut(s)	3679
PaeI	GCATGC	2 cut(s)	994, 3325
PagI	TCATGA	1 cut(s)	835
PaqCI	CACCTGC	1 cut(s)	733
PceI	AGGCCT	1 cut(s)	3143
PciI	ACATGT	1 cut(s)	2919
PctI	GAATGC	2 cut(s)	2127, 2876
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	6 cut(s)	475, 782, 2404, 2668, 2694, 3259
PkrI	GCNGC	9 cut(s)	277, 1369, 1372, 1562, 1711, 1726, 2458, 2564, 3178
Ple19I	CGATCG	1 cut(s)	1942
PleI	GAGTC	5 cut(s)	992, 1597, 1702, 2059, 3322
PpsI	GAGTC	5 cut(s)	992, 1597, 1702, 2059, 3322
Ppu21I	YACGTR	1 cut(s)	3255
PpuMI	RGGWCCY	1 cut(s)	3032
PscI	ACATGT	1 cut(s)	2919
PshBI	ATTAAT	1 cut(s)	2468
PsiI	TTATAA	1 cut(s)	165
Psp1406I	AACGTT	1 cut(s)	204
Psp5II	RGGWCCY	1 cut(s)	3032
Psp6I	CCWGG	2 cut(s)	1248, 3138
PspEI	GGTNACC	1 cut(s)	1078
PspFI	CCCAGC	3 cut(s)	107, 2009, 3013
PspGI	CCWGG	2 cut(s)	1248, 3138
PspN4I	GGNNCC	3 cut(s)	2208, 3187, 3299
PspPI	GGNCC	3 cut(s)	3032, 3377, 3478
PspPPI	RGGWCCY	1 cut(s)	3032
PstI	CTGCAG	1 cut(s)	526
PsuI	RGATCY	3 cut(s)	691, 2247, 2446
PvuI	CGATCG	1 cut(s)	1942
PvuII	CAGCTG	1 cut(s)	2180
RseI	CAYNNNNRTG	2 cut(s)	1992, 3679
SaqAI	TTAA	7 cut(s)	411, 1167, 1286, 1832, 2468, 2489, 3311
SatI	GCNGC	9 cut(s)	276, 1368, 1371, 1561, 1710, 1725, 2457, 2563, 3177
Sau96I	GGNCC	3 cut(s)	3032, 3377, 3478
ScaI	AGTACT	1 cut(s)	2060
SchI	GAGTC	5 cut(s)	992, 1598, 1703, 2060, 3322
ScrFI	CCNGG	2 cut(s)	1250, 3140
SduI	GDGCHC	1 cut(s)	2211
SfaNI	GCATC	7 cut(s)	73, 2527, 2558, 3213, 3274, 3589, 3649
SfcI	CTRYAG	3 cut(s)	522, 2175, 2181
SfuI	TTCGAA	1 cut(s)	785
SinI	GGWCC	3 cut(s)	3032, 3377, 3478
SmiMI	CAYNNNNRTG	2 cut(s)	1992, 3679
SmlI	CTYRAG	6 cut(s)	728, 775, 2045, 2126, 2243, 3616
SmoI	CTYRAG	6 cut(s)	728, 775, 2045, 2126, 2243, 3616
SphI	GCATGC	2 cut(s)	994, 3325
SseBI	AGGCCT	1 cut(s)	3143
SsiI	CCGC	5 cut(s)	885, 1083, 1561, 2384, 2456
SspI	AATATT	1 cut(s)	2842
SspMI	CTAG	7 cut(s)	1386, 1487, 1851, 2135, 2730, 3036, 3362
StuI	AGGCCT	1 cut(s)	3143
StyD4I	CCNGG	2 cut(s)	1248, 3138
StyI	CCWWGG	2 cut(s)	100, 464
TaaI	ACNGT	6 cut(s)	124, 173, 1403, 1495, 2546, 3009
TaiI	ACGT	7 cut(s)	207, 215, 421, 1505, 2363, 2633, 3257
TaqII	GACCGA	1 cut(s)	3365
TatI	WGTACW	2 cut(s)	1821, 2058
TauI	GCSGC	2 cut(s)	1563, 2459
TfiI	GAWTC	6 cut(s)	475, 782, 2404, 2668, 2694, 3259
Tru1I	TTAA	7 cut(s)	411, 1167, 1286, 1832, 2468, 2489, 3311
Tru9I	TTAA	7 cut(s)	411, 1167, 1286, 1832, 2468, 2489, 3311
TscAI	CASTG	6 cut(s)	321, 1357, 1648, 1815, 2219, 2704
TseFI	GTSAC	5 cut(s)	1078, 1388, 1695, 2732, 3672
TseI	GCWGC	7 cut(s)	275, 1367, 1370, 1709, 1724, 2562, 3176
Tsp45I	GTSAC	5 cut(s)	1078, 1388, 1695, 2732, 3672
TspGWI	ACGGA	4 cut(s)	1043, 1234, 1636, 3137
TspRI	CASTG	6 cut(s)	321, 1357, 1648, 1815, 2219, 2704
VpaK11BI	GGWCC	3 cut(s)	3032, 3377, 3478
VspI	ATTAAT	1 cut(s)	2468
XapI	RAATTY	7 cut(s)	601, 856, 983, 1228, 1844, 2350, 3357
XceI	RCATGY	7 cut(s)	994, 1451, 2304, 2456, 2923, 3325, 3538
XmiI	GTMKAC	2 cut(s)	1254, 3090
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	7 cut(s)	1386, 1487, 1851, 2135, 2730, 3036, 3362
ZrmI	AGTACT	1 cut(s)	2060

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.