Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Forward (+)

60732603 .. 60738259

Gene Structure 5657 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020069

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3681 bp

ATGGCTGGAGGAAGAATAAGGGCAAGGCTACGGCGGAGCCACCTCTACACATTCTTGCGTAAGCCAAGGGGCAATGAGTCAGGGGCACCTCATCCAATCCAAGGTCCTGGGTACTCGAGAACTGTACATTGTAACCAACCTCTGCTCCATCAAAAGAAACCTTACAAATACCGTTCAAATTACATATCGACCACAAAGTATAACCCGATCACGTTCTTGCCCAAGGCACTCTTTGAGCAGTTCAGACGGGTTGCCAATATCTATTTCCTTTTGGCTGCTGGTCTTTCACTTACTTCTGTTTCTCCATTCGGTCCTTGGAGCATGATAGCTCCTTTGGCTTTTGTTGTTGGTCTCAGTATGGCAAAGGAAGCTATGGAAGACTGGAATAGGTTCTTGCAGGACATGAAGGTTAATCTCCGGAAAGTAAATGTTCATAAAGGAGATGGTGTTTTCGGATATAGGCCATGGCATAAGATTGTGGTTGGAGATGTGGTGAAAGTGGAAAAGGATAAATTCTTTCCTGCTGATCTGCTTCTATTGTCGTCAAGTTACGAGGATGGTTTCTGCTATGTTGAGACTATGAACTTAGATGGCGAGACAAATTTGAAGGTCAAGAGATGCTTGGAGGTAACCGCACCTTTGGATGATGATGGAGTTTTCAAGGACTTCACAGCAACAGTTCAATGTGAAGATCCAAATCCCAATCTTTATTCCTTTGTGGGTAATCTTGATCATGACCGGCAGATTTATCCTCTTGAACCCGGTCAGATACTCCTGAGAGATTCAAAGCTCAGGAATACAAGTTATATCTATGGGGTAGTGATATTTACAGGTCATGATAGCAAAGTCATGCAGAATTCGACAAAGTCCCCCTCAAAAAGGAGTGGAATAGAAAAGAAAATGGACAAGATTATCTACATCCTTTTCTTCATCCTTTTAGTGATCTCCTTGATCAGCTCAATAGGGTTTGGTGTGAAGACAAGAAAAGATATGCCATCGGCATGGTACTTACGACCAGATGATGCAGAAGATATGTATAGCCCAAAAAAACCTGCTGCGGCTGGCGCTATCCATCTGGTCACTGCTCTCATCCTTTATGGATATCTAATACCCATCTCTCTTTATGTTTCCATTGAGGTTGTGAAGGTCTTGCAAGCAAAGTTCATTAACAATGACATACACATGTATGATGAAGAGCATGGAACTCCTGCTCAAGCCCGGACATCAAATTTGAATGAGGAGTTAGGCCAGGTAGACACGATCCTTTCTGACAAAACTGGCACTTTAACCTGCAACCAGATGAACTTTCTGAAGTGTTGCATTGCTGGCATTGCATACGGTGTCCGCTCTAGTGAAGTTGAACTTGCAGCAGCAAAACAGATGGCTACTGACCTTGATGATCAGGATGAGCATGATGAAGAACTAGCCAATGTTCCCAGGAATAAACATGGTCAACAAGTATCATGGGGGAATGAAGTAGGATCAGAAATTGAGCTGGAAACTGTTGTTACATGCAAGGATGGTAAGGATCCGAAACCTGCCATAAAAGGGTTTAGCTTTGTGGACAACCGCCTCATGGATGGAAATTGGTTGAGAGAGCCCACCTGTGATGTGATTTTATTATTTTTGCGGATTTTAGCCGTTTGCCATACTGCAATTCCTGAGCTAAATGAAGAGACTGGCCAATATACATATGAAGCAGAGTCACCCGACGAAGGAGCTTTTCTTGTTGCAGCACGTGAGTTAGGTTTTGAGTTCTGTAAAAGAAATCAGTCAAGTGTGTATGTCCGCGAAAAGTATCCTGATCCAGGACAACCAGTGGAAAGGGAGTATCAAGTCTTAAATCTATTGGAATTCACCAGCAAAAGGAAGCGTATGTCAGTAATTGTGCGTGACGAAGATGGGCAATTATTACTTTTCTGCAAAGGTGCTGACAGCATCATTTTTGATCGACTGTCAAAGAATGGAAGAATTTATGAGGAAGCTAGTACCAAGCATTTGAATGAATATGGAGAAGCTGGGTTGCGTACGTTGGCACTTGCTTATAAAAAGCTTGAGGAGTCCGAGTATTCTGCTTGGAACAACGAATTTCAGAAAGCAAAATCATCTATTGGGGCTGATAGGGAGGTGATGCTCGAGCTAGTAGCAGAGAAGATGGAAAAAGACTTGATCATGGTTGGTGCTACAGCTGTGGAGGACAAATTGCAAAAAGGGGTGCCCCAATGCATAGACAATCTTGCACAAGCTGGTCTCAAGATCTGGGTGTTGACAGGGGATAAGATGGAAACTGCAATCAACATAGGATTTGCATGTAGTTTGCTCCGACAGGGCATGAAGCAGATCTGTATATCAACAGCAAATTTAGACACATTGGGACAAGATGGAAAAGAGGCTGTGAAAGACAACATTTTGAATCAAATTACAAATGCCTCTCAAATGATCAAGTTGGAAACAGATCCACATGCAGCGTTTGCACTAATTATTGATGGAAAAACTTTAACCTATGCTTTGGACGCTGATATGAAGCATCTATTCTTGGAATTAGCTGTTGATTGTGCATCTGTCATATGCTGTCGTGTCTCCCCTAAGCAGAAGGCATTGGTAACAAGGTTAGTAAGACAAGGAACTGGGAAGACTACATTAGCAATTGGTGATGGTGCAAATGATGTTGGAATGATTCAAGAAGCTGATATTGGTGTTGGAATCAGTGGCGTCGAAGGTATGCAGGCTGTGATGGCTAGTGACTTTGCTATTGCCCAGTTTCGGTTTTTGGAGAGACTTTTGGTTGTTCATGGACATTGGTGCTATAAAAGGATTGCTCAGATGATTTGCTATTTCTTCTACAAAAATATAGCATTTGGTCTGACCCTCTTCTACTTTGAGGCATTCACGGGATTTTCGGGGCAATCAATATATGCTGACTTGTACATGCTAACGTTCAATGTTATTCTTACCTCATTGCCTGTCATATCACTTGGAGTATTTGAACAAGATGTTTCTTCCGAGGTCTGCTTACAGTTCCCTGCATTATATCAGCAAGGAGCCCGAAATTTGTTCTTCGACTGGTACAGAATACTGGGGTGGATGGGCAATGGTGTATATTGCTCTCTCATTGTCTTCTTCCTCAACATTATCATATTCAAAGACCAGTCCTTCAGGTCTGATGGCCAGATTGCTGATATGGCTGCTATGGGTACAACTATGTTCAGTTGCATCATATGGGCCGTCAACTGCCAGATTGCTCTGGCAATGTGTCACTTTACATGGATTCAACACTGCTTTATTTGGGGCAGTATTGCAATGTGGTACCTATTTCTCTTAATTTACGGCATAGTGACACCAACGTGGTCTGGAAATGCCTACCAGATTCTAGTTGAAGTCCTCGGTCCTGCACCTATCTTTTGGTCAGCCACCCTATTAGTTACTCTTGCTTGTAATCTCCCTTACATTGTCCACATAGCCTTCCAAAGAAGTTTCAATCCAATGGATCATCATATCATCCAAGAAATCAAATACTACCGGAAAGATGTTCAGGACCAACGCATGTGGAAGAGGGAGGCATCTAAAGCAAGACAAGAAACCAAGATTGGGTTCTCAGCTCGGGTAGATGCAAAGATCAGACAATTGAGGGCGAGGCTGCACAAGAAGCAACCCCAGAGTAACGCTATGTCCCCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1227

Amino Acids

138.21

Weight (kDa)

6.61

Isoelectric Point (pI)

37.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	42 - 109	7.5e-24	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	542 - 737	5.6e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	911 - 1161	9.1e-88	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2046
Acc36I	ACCTGC	3 cut(s)	1060, 1298, 1546
Acc65I	GGTACC	1 cut(s)	3306
AccB1I	GGYRCC	3 cut(s)	85, 2215, 3306
AccBSI	CCGCTC	1 cut(s)	1347
AccI	GTMKAC	1 cut(s)	1254
AccII	CGCG	1 cut(s)	1791
AccIII	TCCGGA	1 cut(s)	417
AciI	CCGC	7 cut(s)	34, 633, 1058, 1345, 1570, 1630, 1789
AclI	AACGTT	1 cut(s)	2936
AclWI	GGATC	8 cut(s)	686, 1255, 1489, 1523, 1536, 1799, 2450, 3495
AcoI	YGGCCR	2 cut(s)	1681, 3166
AcsI	RAATTY	9 cut(s)	512, 601, 856, 1228, 1853, 1971, 2087, 2359, 3049
AcuI	CTGAAG	2 cut(s)	1331, 3139
AcvI	CACGTG	1 cut(s)	1739
AcyI	GRCGYC	1 cut(s)	2712
AfaI	GTAC	9 cut(s)	113, 126, 1007, 1990, 2029, 2927, 3068, 3196, 3308
AfiI	CCNNNNNNNGG	9 cut(s)	101, 879, 1548, 1576, 1715, 1808, 1866, 2763, 3588
AflIII	ACRYGT	1 cut(s)	1182
AjnI	CCWGG	4 cut(s)	106, 1248, 1436, 1807
AjuI	GAANNNNNNNTTGG	8 cut(s)	248, 280, 2676, 2708, 3570, 3575, 3602, 3607
AleI	CACNNNNGTG	1 cut(s)	3343
Alw26I	GTCTC	7 cut(s)	356, 569, 590, 1670, 2255, 2584, 2770
AlwI	GGATC	8 cut(s)	686, 1255, 1489, 1523, 1536, 1799, 2450, 3495
Ama87I	CYCGRG	3 cut(s)	115, 2135, 3600
Aor13HI	TCCGGA	1 cut(s)	417
AoxI	GGCC	5 cut(s)	461, 1246, 1681, 3166, 3222
ApeKI	GCWGC	8 cut(s)	275, 1055, 1367, 1370, 1733, 2465, 3185, 3637
ApoI	RAATTY	9 cut(s)	512, 601, 856, 1228, 1853, 1971, 2087, 2359, 3049
ArsI	GACNNNNNNTTYG	2 cut(s)	1213, 1245
Asp700I	GAANNNNTTC	1 cut(s)	389
Asp718I	GGTACC	1 cut(s)	3306
AspLEI	GCGC	1 cut(s)	1067
AspS9I	GGNCC	5 cut(s)	104, 311, 3222, 3386, 3535
AsuC2I	CCSGG	2 cut(s)	762, 1219
AsuHPI	GGTGA	5 cut(s)	505, 1698, 1849, 2140, 2663
AvaI	CYCGRG	3 cut(s)	115, 2135, 3600
AvaII	GGWCC	4 cut(s)	104, 311, 3386, 3535
BaeGI	GKGCMC	2 cut(s)	88, 2220
BalI	TGGCCA	2 cut(s)	1683, 3168
BamHI	GGATCC	1 cut(s)	1528
BanI	GGYRCC	3 cut(s)	85, 2215, 3306
BanII	GRGCYC	2 cut(s)	1602, 3046
BbrPI	CACGTG	1 cut(s)	1739
BbsI	GAAGAC	4 cut(s)	384, 983, 2639, 3109
BbvI	GCAGC	8 cut(s)	262, 1042, 1379, 1382, 1745, 2477, 3172, 3624
BceAI	ACGGC	4 cut(s)	47, 1625, 3209, 3343
BcgI	CGANNNNNNTGC	2 cut(s)	2054, 2088
BciT130I	CCWGG	4 cut(s)	108, 1250, 1438, 1809
BciVI	GTATCC	1 cut(s)	1809
BclI	TGATCA	5 cut(s)	730, 951, 1399, 2169, 2439
BcnI	CCSGG	2 cut(s)	762, 1219
BcoDI	GTCTC	7 cut(s)	356, 569, 590, 1670, 2255, 2584, 2770
BfaI	CTAG	6 cut(s)	1350, 1424, 1986, 2141, 2739, 3371
BfmI	CTRYAG	1 cut(s)	2184
BfoI	RGCGCY	1 cut(s)	1068
BfuAI	ACCTGC	3 cut(s)	1060, 1298, 1546
BfuI	GTATCC	1 cut(s)	1809
BglII	AGATCT	2 cut(s)	2256, 2340
BisI	GCNGC	9 cut(s)	276, 1056, 1059, 1368, 1371, 1734, 2466, 3186, 3638
BlsI	GCNGC	9 cut(s)	277, 1057, 1060, 1369, 1372, 1735, 2467, 3187, 3639
Bme1390I	CCNGG	6 cut(s)	108, 762, 1219, 1250, 1438, 1809
Bme18I	GGWCC	4 cut(s)	104, 311, 3386, 3535
BmeT110I	CYCGRG	3 cut(s)	115, 2135, 3600
BmgT120I	GGNCC	5 cut(s)	104, 311, 3222, 3386, 3535
BmiI	GGNNCC	6 cut(s)	38, 87, 1530, 2217, 3043, 3308
BmrFI	CCNGG	6 cut(s)	108, 762, 1219, 1250, 1438, 1809
BmrI	ACTGGG	3 cut(s)	2637, 2752, 3086
BmsI	GCATC	9 cut(s)	608, 1012, 1947, 2121, 2536, 2567, 3222, 3569, 3598
BmuI	ACTGGG	3 cut(s)	2637, 2752, 3086
BpiI	GAAGAC	4 cut(s)	384, 983, 2639, 3109
BpmI	CTGGAG	1 cut(s)	27
Bpu10I	CCTNAGC	3 cut(s)	791, 1662, 2586
BpuEI	CTTGAG	3 cut(s)	1197, 2075, 2237
BpuMI	CCSGG	2 cut(s)	762, 1219
BsaAI	YACGTR	1 cut(s)	1739
BsaBI	GATNNNNATC	1 cut(s)	696
BsaHI	GRCGYC	1 cut(s)	2712
BsaI	GGTCTC	2 cut(s)	356, 2255
BsaJI	CCNNGG	9 cut(s)	65, 100, 107, 222, 314, 464, 1436, 3003, 3382
BsaWI	WCCGGW	2 cut(s)	417, 3519
BsaXI	ACNNNNNCTCC	8 cut(s)	129, 159, 432, 462, 477, 507, 2051, 2081
Bsc4I	CCNNNNNNNGG	9 cut(s)	101, 879, 1548, 1576, 1715, 1808, 1866, 2763, 3588
Bse118I	RCCGGY	1 cut(s)	738
Bse1I	ACTGG	9 cut(s)	386, 1282, 1684, 1817, 2632, 2758, 3068, 3081, 3148
Bse3DI	GCAATG	7 cut(s)	79, 1320, 1329, 2957, 3097, 3255, 3306
Bse8I	GATNNNNATC	1 cut(s)	696
BseAI	TCCGGA	1 cut(s)	417
BseBI	CCWGG	4 cut(s)	108, 1250, 1438, 1809
BseDI	CCNNGG	9 cut(s)	65, 100, 107, 222, 314, 464, 1436, 3003, 3382
BseJI	GATNNNNATC	1 cut(s)	696
BseLI	CCNNNNNNNGG	9 cut(s)	101, 879, 1548, 1576, 1715, 1808, 1866, 2763, 3588
BseMI	GCAATG	7 cut(s)	79, 1320, 1329, 2957, 3097, 3255, 3306
BseMII	CTCAG	6 cut(s)	367, 767, 805, 1653, 2834, 3609
BseNI	ACTGG	9 cut(s)	386, 1282, 1684, 1817, 2632, 2758, 3068, 3081, 3148
BseRI	GAGGAG	2 cut(s)	1253, 2072
BseSI	GKGCMC	2 cut(s)	88, 2220
BseXI	GCAGC	8 cut(s)	262, 1042, 1379, 1382, 1745, 2477, 3172, 3624
BseYI	CCCAGC	1 cut(s)	2018
BsgI	GTGCAG	2 cut(s)	3375, 3623
Bsh1236I	CGCG	1 cut(s)	1791
BshFI	GGCC	5 cut(s)	463, 1248, 1683, 3168, 3224
BshNI	GGYRCC	3 cut(s)	85, 2215, 3306
BsiHKCI	CYCGRG	3 cut(s)	115, 2135, 3600
BsiSI	CCGG	5 cut(s)	418, 739, 762, 1219, 3520
BsiWI	CGTACG	1 cut(s)	2027
BslFI	GGGAC	3 cut(s)	853, 2388, 3655
BslI	CCNNNNNNNGG	9 cut(s)	101, 879, 1548, 1576, 1715, 1808, 1866, 2763, 3588
BsmAI	GTCTC	7 cut(s)	356, 569, 590, 1670, 2255, 2584, 2770
BsmFI	GGGAC	3 cut(s)	853, 2388, 3655
BsmI	GAATGC	1 cut(s)	2885
BsnI	GGCC	5 cut(s)	463, 1248, 1683, 3168, 3224
Bso31I	GGTCTC	2 cut(s)	356, 2255
BsoBI	CYCGRG	3 cut(s)	115, 2135, 3600
Bsp1286I	GDGCHC	4 cut(s)	88, 1602, 2220, 3046
Bsp13I	TCCGGA	1 cut(s)	417
Bsp1407I	TGTACA	2 cut(s)	124, 2925
Bsp19I	CCATGG	1 cut(s)	464
BspACI	CCGC	7 cut(s)	34, 633, 1058, 1345, 1570, 1630, 1789
BspANI	GGCC	5 cut(s)	463, 1248, 1683, 3168, 3224
BspCNI	CTCAG	6 cut(s)	366, 768, 804, 1654, 2833, 3608
BspEI	TCCGGA	1 cut(s)	417
BspFNI	CGCG	1 cut(s)	1791
BspHI	TCATGA	2 cut(s)	733, 835
BspLI	GGNNCC	6 cut(s)	38, 87, 1530, 2217, 3043, 3308
BspMI	ACCTGC	3 cut(s)	1060, 1298, 1546
BspPI	GGATC	8 cut(s)	686, 1255, 1489, 1523, 1536, 1799, 2450, 3495
BspQI	GCTCTTC	1 cut(s)	1188
BspT107I	GGYRCC	3 cut(s)	85, 2215, 3306
BspTNI	GGTCTC	2 cut(s)	356, 2255
BsrBI	CCGCTC	1 cut(s)	1347
BsrDI	GCAATG	7 cut(s)	79, 1320, 1329, 2957, 3097, 3255, 3306
BsrFI	RCCGGY	1 cut(s)	738
BsrGI	TGTACA	2 cut(s)	124, 2925
BsrI	ACTGG	9 cut(s)	386, 1282, 1684, 1817, 2632, 2758, 3068, 3081, 3148
BssAI	RCCGGY	1 cut(s)	738
BssECI	CCNNGG	9 cut(s)	65, 100, 107, 222, 314, 464, 1436, 3003, 3382
BssNI	GRCGYC	1 cut(s)	2712
BssT1I	CCWWGG	5 cut(s)	65, 100, 222, 314, 464
Bst2UI	CCWGG	4 cut(s)	108, 1250, 1438, 1809
Bst4CI	ACNGT	7 cut(s)	124, 173, 679, 1340, 1504, 1956, 3018
Bst6I	CTCTTC	4 cut(s)	1188, 1668, 2876, 3545
BstACI	GRCGYC	1 cut(s)	2712
BstAPI	GCANNNNNTGC	1 cut(s)	2471
BstAUI	TGTACA	2 cut(s)	124, 2925
BstBAI	YACGTR	1 cut(s)	1739
BstC8I	GCNNGC	4 cut(s)	1063, 1155, 1327, 2727
BstDEI	CTNAG	8 cut(s)	353, 586, 776, 791, 1662, 2586, 2820, 3595
BstDSI	CCRYGG	1 cut(s)	464
BstEII	GGTNACC	1 cut(s)	628
BstENI	CCTNNNNNAGG	2 cut(s)	877, 1806
BstFNI	CGCG	1 cut(s)	1791
BstH2I	RGCGCY	1 cut(s)	1068
BstHHI	GCGC	1 cut(s)	1067
BstMAI	GTCTC	7 cut(s)	356, 569, 590, 1670, 2255, 2584, 2770
BstNI	CCWGG	4 cut(s)	108, 1250, 1438, 1809
BstNSI	RCATGY	6 cut(s)	1186, 1515, 2313, 2465, 2932, 3547
BstPI	GGTNACC	1 cut(s)	628
BstSCI	CCNGG	6 cut(s)	106, 760, 1217, 1248, 1436, 1807
BstSFI	CTRYAG	1 cut(s)	2184
BstSLI	GKGCMC	2 cut(s)	88, 2220
BstUI	CGCG	1 cut(s)	1791
BstV1I	GCAGC	8 cut(s)	262, 1042, 1379, 1382, 1745, 2477, 3172, 3624
BstV2I	GAAGAC	4 cut(s)	384, 983, 2639, 3109
BstX2I	RGATCY	5 cut(s)	691, 1528, 2256, 2340, 2455
BstXI	CCANNNNNNTGG	2 cut(s)	107, 1002
BstYI	RGATCY	5 cut(s)	691, 1528, 2256, 2340, 2455
BsuI	GTATCC	1 cut(s)	1809
BsuRI	GGCC	5 cut(s)	463, 1248, 1683, 3168, 3224
BtgI	CCRYGG	1 cut(s)	464
BtsI	GCAGTG	2 cut(s)	1080, 3274
BtsIMutI	CAGTG	4 cut(s)	1080, 1824, 2713, 3274
BveI	ACCTGC	3 cut(s)	1060, 1298, 1546
Cac8I	GCNNGC	4 cut(s)	1063, 1155, 1327, 2727
CciI	TCATGA	2 cut(s)	733, 835
CfoI	GCGC	1 cut(s)	1067
Cfr10I	RCCGGY	1 cut(s)	738
Cfr13I	GGNCC	5 cut(s)	104, 311, 3222, 3386, 3535
CseI	GACGC	2 cut(s)	2522, 2701
Csp6I	GTAC	9 cut(s)	112, 125, 1006, 1989, 2028, 2926, 3067, 3195, 3307
CspCI	CAANNNNNGTGG	2 cut(s)	3527, 3562
CviQI	GTAC	9 cut(s)	112, 125, 1006, 1989, 2028, 2926, 3067, 3195, 3307
DdeI	CTNAG	8 cut(s)	353, 586, 776, 791, 1662, 2586, 2820, 3595
EaeI	YGGCCR	2 cut(s)	1681, 3166
Eam1104I	CTCTTC	4 cut(s)	1188, 1668, 2876, 3545
EarI	CTCTTC	4 cut(s)	1188, 1668, 2876, 3545
EciI	GGCGGA	1 cut(s)	49
Eco130I	CCWWGG	5 cut(s)	65, 100, 222, 314, 464
Eco24I	GRGCYC	2 cut(s)	1602, 3046
Eco31I	GGTCTC	2 cut(s)	356, 2255
Eco32I	GATATC	1 cut(s)	1103
Eco47I	GGWCC	4 cut(s)	104, 311, 3386, 3535
Eco57I	CTGAAG	2 cut(s)	1331, 3139
Eco72I	CACGTG	1 cut(s)	1739
Eco88I	CYCGRG	3 cut(s)	115, 2135, 3600
Eco91I	GGTNACC	1 cut(s)	628
EcoNI	CCTNNNNNAGG	2 cut(s)	877, 1806
EcoO109I	RGGNCCY	1 cut(s)	104
EcoO65I	GGTNACC	1 cut(s)	628
EcoRI	GAATTC	2 cut(s)	856, 1853
EcoRII	CCWGG	4 cut(s)	106, 1248, 1436, 1807
EcoRV	GATATC	1 cut(s)	1103
EcoT14I	CCWWGG	5 cut(s)	65, 100, 222, 314, 464
EcoT22I	ATGCAT	1 cut(s)	2228
EcoT38I	GRGCYC	2 cut(s)	1602, 3046
ErhI	CCWWGG	5 cut(s)	65, 100, 222, 314, 464
FalI	AAGNNNNNCTT	6 cut(s)	215, 247, 605, 637, 1347, 1379
FaqI	GGGAC	3 cut(s)	853, 2388, 3655
FauNDI	CATATG	3 cut(s)	1693, 2567, 3218
FbaI	TGATCA	5 cut(s)	730, 951, 1399, 2169, 2439
FblI	GTMKAC	1 cut(s)	1254
Fnu4HI	GCNGC	9 cut(s)	276, 1056, 1059, 1368, 1371, 1734, 2466, 3186, 3638
FriOI	GRGCYC	2 cut(s)	1602, 3046
Fsp4HI	GCNGC	9 cut(s)	276, 1056, 1059, 1368, 1371, 1734, 2466, 3186, 3638
FspBI	CTAG	6 cut(s)	1350, 1424, 1986, 2141, 2739, 3371
GlaI	GCGC	1 cut(s)	1066
GluI	GCNGC	9 cut(s)	276, 1056, 1059, 1368, 1371, 1734, 2466, 3186, 3638
GsaI	CCCAGC	1 cut(s)	2022
GsuI	CTGGAG	1 cut(s)	27
HaeII	RGCGCY	1 cut(s)	1068
HaeIII	GGCC	5 cut(s)	463, 1248, 1683, 3168, 3224
HapII	CCGG	5 cut(s)	418, 739, 762, 1219, 3520
HgaI	GACGC	2 cut(s)	2522, 2701
HhaI	GCGC	1 cut(s)	1067
Hin1I	GRCGYC	1 cut(s)	2712
Hin6I	GCGC	1 cut(s)	1065
HinP1I	GCGC	1 cut(s)	1065
HincII	GTYRAC	3 cut(s)	1454, 2268, 3229
HindII	GTYRAC	3 cut(s)	1454, 2268, 3229
HindIII	AAGCTT	1 cut(s)	2051
HinfI	GANTC	9 cut(s)	77, 782, 1703, 2060, 2413, 2677, 2703, 3268, 3367
HpaII	CCGG	5 cut(s)	418, 739, 762, 1219, 3520
HphI	GGTGA	5 cut(s)	505, 1698, 1849, 2140, 2663
Hpy166II	GTNNAC	6 cut(s)	1255, 1454, 1564, 2268, 3229, 3454
Hpy8I	GTNNAC	6 cut(s)	1255, 1454, 1564, 2268, 3229, 3454
Hpy99I	CGWCG	2 cut(s)	1715, 2717
HpyAV	CCTTC	8 cut(s)	400, 601, 1138, 1709, 2587, 2711, 3163, 3472
HpyCH4III	ACNGT	7 cut(s)	124, 173, 679, 1340, 1504, 1956, 3018
HpyCH4IV	ACGT	5 cut(s)	212, 1738, 2030, 2936, 3344
HpyF3I	CTNAG	8 cut(s)	353, 586, 776, 791, 1662, 2586, 2820, 3595
HpySE526I	ACGT	5 cut(s)	212, 1738, 2030, 2936, 3344
Hsp92I	GRCGYC	1 cut(s)	2712
HspAI	GCGC	1 cut(s)	1065
Kpn2I	TCCGGA	1 cut(s)	417
KpnI	GGTACC	1 cut(s)	3310
Ksp22I	TGATCA	5 cut(s)	730, 951, 1399, 2169, 2439
LguI	GCTCTTC	1 cut(s)	1188
LmnI	GCTCC	7 cut(s)	36, 150, 318, 334, 1718, 2325, 3041
Lsp1109I	GCAGC	8 cut(s)	262, 1042, 1379, 1382, 1745, 2477, 3172, 3624
LweI	GCATC	9 cut(s)	608, 1012, 1947, 2121, 2536, 2567, 3222, 3569, 3598
MaeI	CTAG	6 cut(s)	1350, 1424, 1986, 2141, 2739, 3371
MaeII	ACGT	5 cut(s)	212, 1738, 2030, 2936, 3344
MbiI	CCGCTC	1 cut(s)	1347
MfeI	CAATTG	2 cut(s)	2646, 3623
MflI	RGATCY	5 cut(s)	691, 1528, 2256, 2340, 2455
MhlI	GDGCHC	4 cut(s)	88, 1602, 2220, 3046
MlsI	TGGCCA	2 cut(s)	1683, 3168
MluNI	TGGCCA	2 cut(s)	1683, 3168
MlyI	GAGTC	3 cut(s)	86, 1712, 2069
MmeI	TCCRAC	5 cut(s)	463, 2347, 2427, 2650, 2680
Mox20I	TGGCCA	2 cut(s)	1683, 3168
Mph1103I	ATGCAT	1 cut(s)	2228
MroI	TCCGGA	1 cut(s)	417
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	2 cut(s)	1683, 3168
MseI	TTAA	6 cut(s)	411, 1167, 1286, 1841, 2498, 3320
MslI	CAYNNNNRTG	5 cut(s)	1000, 1181, 1185, 2001, 3343
Msp20I	TGGCCA	2 cut(s)	1683, 3168
MspA1I	CMGCKG	1 cut(s)	2189
MspI	CCGG	5 cut(s)	418, 739, 762, 1219, 3520
MspR9I	CCNGG	6 cut(s)	108, 762, 1219, 1250, 1438, 1809
MunI	CAATTG	2 cut(s)	2646, 3623
Mva1269I	GAATGC	1 cut(s)	2885
MvaI	CCWGG	4 cut(s)	108, 1250, 1438, 1809
MvnI	CGCG	1 cut(s)	1791
NciI	CCSGG	2 cut(s)	762, 1219
NcoI	CCATGG	1 cut(s)	464
NdeI	CATATG	3 cut(s)	1693, 2567, 3218
NlaIV	GGNNCC	6 cut(s)	38, 87, 1530, 2217, 3043, 3308
NmuCI	GTSAC	6 cut(s)	1078, 1704, 1892, 2741, 3254, 3334
NsiI	ATGCAT	1 cut(s)	2228
NspI	RCATGY	6 cut(s)	1186, 1515, 2313, 2465, 2932, 3547
OliI	CACNNNNGTG	1 cut(s)	3343
PaeR7I	CTCGAG	2 cut(s)	115, 2135
PagI	TCATGA	2 cut(s)	733, 835
PciI	ACATGT	1 cut(s)	1182
PciSI	GCTCTTC	1 cut(s)	1188
PctI	GAATGC	1 cut(s)	2885
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	6 cut(s)	782, 2413, 2677, 2703, 3268, 3367
Pfl23II	CGTACG	1 cut(s)	2027
PflFI	GACNNNGTC	1 cut(s)	865
PfoI	TCCNGGA	1 cut(s)	1807
PkrI	GCNGC	9 cut(s)	277, 1057, 1060, 1369, 1372, 1735, 2467, 3187, 3639
PleI	GAGTC	3 cut(s)	85, 1711, 2068
PmaCI	CACGTG	1 cut(s)	1739
PmlI	CACGTG	1 cut(s)	1739
PpsI	GAGTC	3 cut(s)	85, 1711, 2068
Ppu21I	YACGTR	1 cut(s)	1739
PpuMI	RGGWCCY	1 cut(s)	104
PscI	ACATGT	1 cut(s)	1182
PsiI	TTATAA	1 cut(s)	2046
Psp1406I	AACGTT	1 cut(s)	2936
Psp5II	RGGWCCY	1 cut(s)	104
Psp6I	CCWGG	4 cut(s)	106, 1248, 1436, 1807
PspCI	CACGTG	1 cut(s)	1739
PspEI	GGTNACC	1 cut(s)	628
PspFI	CCCAGC	1 cut(s)	2018
PspGI	CCWGG	4 cut(s)	106, 1248, 1436, 1807
PspLI	CGTACG	1 cut(s)	2027
PspN4I	GGNNCC	6 cut(s)	38, 87, 1530, 2217, 3043, 3308
PspPI	GGNCC	5 cut(s)	104, 311, 3222, 3386, 3535
PspPPI	RGGWCCY	1 cut(s)	104
PspXI	VCTCGAGB	1 cut(s)	2135
PsuI	RGATCY	5 cut(s)	691, 1528, 2256, 2340, 2455
PsyI	GACNNNGTC	1 cut(s)	865
PvuII	CAGCTG	1 cut(s)	2189
RsaI	GTAC	9 cut(s)	113, 126, 1007, 1990, 2029, 2927, 3068, 3196, 3308
RsaNI	GTAC	9 cut(s)	112, 125, 1006, 1989, 2028, 2926, 3067, 3195, 3307
RseI	CAYNNNNRTG	5 cut(s)	1000, 1181, 1185, 2001, 3343
SapI	GCTCTTC	1 cut(s)	1188
SaqAI	TTAA	6 cut(s)	411, 1167, 1286, 1841, 2498, 3320
SatI	GCNGC	9 cut(s)	276, 1056, 1059, 1368, 1371, 1734, 2466, 3186, 3638
Sau96I	GGNCC	5 cut(s)	104, 311, 3222, 3386, 3535
SchI	GAGTC	3 cut(s)	86, 1712, 2069
ScrFI	CCNGG	6 cut(s)	108, 762, 1219, 1250, 1438, 1809
SduI	GDGCHC	4 cut(s)	88, 1602, 2220, 3046
SfaNI	GCATC	9 cut(s)	608, 1012, 1947, 2121, 2536, 2567, 3222, 3569, 3598
SfcI	CTRYAG	1 cut(s)	2184
Sfr274I	CTCGAG	2 cut(s)	115, 2135
SinI	GGWCC	4 cut(s)	104, 311, 3386, 3535
SlaI	CTCGAG	2 cut(s)	115, 2135
SmiMI	CAYNNNNRTG	5 cut(s)	1000, 1181, 1185, 2001, 3343
SmlI	CTYRAG	5 cut(s)	115, 1212, 2054, 2135, 2252
SmoI	CTYRAG	5 cut(s)	115, 1212, 2054, 2135, 2252
SsiI	CCGC	7 cut(s)	34, 633, 1058, 1345, 1570, 1630, 1789
SspMI	CTAG	6 cut(s)	1350, 1424, 1986, 2141, 2739, 3371
StyD4I	CCNGG	6 cut(s)	106, 760, 1217, 1248, 1436, 1807
StyI	CCWWGG	5 cut(s)	65, 100, 222, 314, 464
TaaI	ACNGT	7 cut(s)	124, 173, 679, 1340, 1504, 1956, 3018
TaiI	ACGT	5 cut(s)	215, 1741, 2033, 2939, 3347
TaqI	TCGA	7 cut(s)	116, 188, 860, 1951, 2136, 2715, 3060
TaqII	GACCGA	2 cut(s)	299, 3374
TatI	WGTACW	2 cut(s)	124, 2925
TauI	GCSGC	1 cut(s)	1061
TfiI	GAWTC	6 cut(s)	782, 2413, 2677, 2703, 3268, 3367
Tru1I	TTAA	6 cut(s)	411, 1167, 1286, 1841, 2498, 3320
Tru9I	TTAA	6 cut(s)	411, 1167, 1286, 1841, 2498, 3320
TscAI	CASTG	4 cut(s)	1087, 1824, 2713, 3281
TseFI	GTSAC	6 cut(s)	1078, 1704, 1892, 2741, 3254, 3334
TseI	GCWGC	8 cut(s)	275, 1055, 1367, 1370, 1733, 2465, 3185, 3637
Tsp45I	GTSAC	6 cut(s)	1078, 1704, 1892, 2741, 3254, 3334
TspRI	CASTG	4 cut(s)	1087, 1824, 2713, 3281
Tth111I	GACNNNGTC	1 cut(s)	865
VpaK11BI	GGWCC	4 cut(s)	104, 311, 3386, 3535
XagI	CCTNNNNNAGG	2 cut(s)	877, 1806
XapI	RAATTY	9 cut(s)	512, 601, 856, 1228, 1853, 1971, 2087, 2359, 3049
XceI	RCATGY	6 cut(s)	1186, 1515, 2313, 2465, 2932, 3547
XcmI	CCANNNNNNNNNTGG	1 cut(s)	312
XhoI	CTCGAG	2 cut(s)	115, 2135
XmiI	GTMKAC	1 cut(s)	1254
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	6 cut(s)	1350, 1424, 1986, 2141, 2739, 3371
Zsp2I	ATGCAT	1 cut(s)	2228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.