Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Reverse (-)

17964761 .. 17970829

Gene Structure 6069 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1173300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3684 bp

ATGGCAGGAGGAAAAATAAGGGCGAAGCTTCGCCAGAGTAACCTTTACACATTCTGCCATAAGCCAAAAGCCAATGAGACCGAGGCGCCTCACCCAATCCAAGGCGCCGGCTTCTCAAGAACTGTATATTGTAACCAACCTCTTCTCCATCAAAAGAAGCCTTATAAATACCGTTCAAATTTTATATCAACCACCAAGTATAACCCAATCACCTTCTTGCCCAAAGCACTTTTTGAGCAGTTCAGACGGGTTGCCAATGTGTATTTCCTTTTGGCTGCCATTCTTTCCCTCACACCCGTTTCACCATTCTCTCCAGTGAGTATGATTGCTCCACTGGTATTTGTTGTTGGTCTCAGTATGGCAAAGGAAGCGATGGAAGATTGGAATAGGTTCTTGCAGGATATGAAAGTTAATCGTCGTAAAGTAATTGTTCATAAAGGGGATGGTGATTTTGGTTTTAATCCATGGCATAAGATTTGTGTTGGAGATGTTGTGAAAGTGGAAAAGGATCAATTCTTTCCTGCAGATTTGCTTCTTTTGTCGTCAAGTTATGAGGATGGGATTTGCTATGTTGAGACCATGAACTTAGATGGTGAAACGAATTTGAAGTTAAAGAGATGTTTGGAAGTAACCTCGCCAATGGAGGATGATGGCGCTTTCAAGGATTTGACGGCAACAATTCATTGTGAAGATCCAAACCCAAATCTTTACTCCTTCATAGGTAATCTTGAGTATGATCGGCAGGTGTATCCTATTGAACCCAATCAGATACTCTTGAGAGATTCGAAGCTCAGGAATACAGCTTATGTGTATGGGGTGGTGATATTCACGGGTCACGATAGCAAAGTCATGCAGAATTCAACGAAGTCCCCTTCAAAAAGGAGCGGAATAGAAAGGAAAATGGATAACATTATTTATATTCTTTTCACCCTCCTTGTAGGGATTTCAGTGATCAGCTCAATAGGCTTTGCCGTGAAGACAAAATTCAGCATGCCAGACGCGTGGTACTTACGACCTGATAATACAACGGATATGTATAGCCCAAAAAAGCCTGCCTTGTCCGGCCTTATCCATTTGATCACTGCTCTCATCCTTTATGGATATCTAATACCCATCTCTCTCTATGTTTCAATTGAGGTTGTGAAGGTTTTGCAAGCAAGCTTCATTAACCAAGACTTACTCATGTATGATGAAGAGACTGGGAATCCTGCTCAAGCACGGACATCAAATTTGAATGAAGAGTTAGGCCAGGTAGACACGATCCTTTCTGATAAAACTGGCACGTTAACCTGCAACCAGATGGACTTTCTGAAGTGTTCCATTGCTGGTACTGCATATGGTGTCCGTTCCAGTGAAGTTGAACTTGCAGCAGCAAAACAGATGGCTAGTGACCTTGAAGACCATGATGATGAGCTTGCCAATTTTCCCATGCGAAAACGTAACACACGTTTTTCATGGGAGAAAGGAATAGGATCAGAAATTGAGCTAGAGACAGTTGTTACGTCCAATGAAGAAAATGATCTGAAACCTGCCATAAAGGGGTTTAGCTTTGAGGACAGCCGCCTCATGGTGGGAAATTGGTTGAATGAGTCCAGCCCTGATGTGGTTTCATTGTTTTTACGGATATTAGCAGTTTGCCACACTGCAATTCCCGAGCTAAATGAAGAAACTGGCAGTTATACATATGAAGCAGAGTCACCTGATGAAGCAGCTTTTCTTGTTGCAGCAAGAGAGTTAGGATTTGAGTTTTGTAAAAGAAATCAGTCAGGTGTAATTGTCCGCGAAAGGTATCATCATTCCGGAAAACCAGTGGAAAGGGAGTACAAAATCTTAAATCTATTGGAATTCACTAGCAAAAGGAAGCGTATGTCTGTAATTGTGCGAGATGAAGATGGACAACTTTTACTTATGTGCAAAGGTGCTGACAGCATAATTTTTGATCGACTTTCAAAGAATGGAAGAATGTATGAGGAAGCTACTACTAAGCATTTGAATGAATATGGAGAAGCTGGGTTGCGTACATTGGCACTTGCTTATAGAAAGCTTGAGGAGTCTGAGTACTCTGCTTGGAGCAGTGAGTTTCAGAAAGCAAAAACATCTATTGGGGCTGATAGGGATGGAATGCTTGAGCGAGTAGCAGATAAGATGGAAAGAGACTTGATCCTTGTTGGTGCTACAGCTGTTGAGGACAAATTGCAAAAAGGGGTGCCCCAGTGCATAGACAATCTCGCACAAGCTGGTCTTAAGATCTGGGTTTTGACGGGAGACAAGATGGAAACTGCAATCAACATAGGATTTGCATGTAGTTTGCTCCGACAGGGCATGAAGCAGATCTGTATATCAACAGCAAATTTAGACACGTTGGGACAAGATAGCAAAGAGGCTGTGAAAGATAACATTTTGAACCAAATTACCAATGCCTCACAAATGATCAAGTTGGAAAAAGATCCGCATGCAGCATTTGCATTAATCATCGATGGAAAAACTTTAAGCTACGCTTTGGAGGATGATATGAAACATCTATTCTTAGGATTAGGTGTTGACTGTGCATCAGTCATATGCTGCCGTGTCTCTCCCAAGCAGAAGGCATTGGTAACAAGGTTAGTCAAACAAGGCACTGGAAAAACCACATTAGCCATTGGTGACGGTGCAAATGACGTTGGAATGATTCAAGAAGCTGACATTGGTGTTGGAATCAGTGGGGTCGAAGGTATGCAGGCTGTGATGGCTAGTGACTTTGCTATTTCCCAGTTTCGGTTTTTGGAGAGACTTTTGGTAGTACACGGGCATTGGTGCTATAAGAGGATTGCTCAGATGGTTTGCTATTTCTTCTACAAAAACATTGCATTTGGTCTTACCCTCTTCTACTTCGAGGCATTCACTGGTTTTTCTGGGCAATCAATATATGACGACTGGTACATGTTATCATTCAATGTCATTCTAACCTCATTGCCTGTAATTTCGCTTGGAGTTTTTGAACAAGATGTTTCGTCTGAGGTCTGCTTACAGTTCCCTGCATTGTACCAACAAGGACCTAGAAATTTGTTCTTTGACTGGTACAGGATACTGGGGTGGATGGGCAATGGTGTCTATTGCTCTCTCATAGTCTTCTTTCTCAACATCATCATCTTCTACGACCAAGCCTTCCGTGCCAATGGCCAGACAGCTGATATGGCTGCTATGGGTACCACTATGTTCAGTTGCATCGTTTGGGCTGTTAACTGCCAGATTGCTCTCATAACGAGCCACTTTACGTGGATTCAGCATCTCCTTGTGTGGGGAAGTATTGCAATGTGGTACCTATTTCTCTTGCTTTACGGAATGCTGCCTCCAACTCGCTCCAAGAATGCTTACCAAATTCTAGTCGAAGCTCTTGGTCCTGCCCCATTATTTTGGTCAGCCACTCTCTTAGTAACAATCGCATGTAATCTCCCATACATTGTCCACTTAGCATTCCAAAGAAGTTTCAATCCAATGGATCATCATATCATCCAAGAAATCAAGTACTACAAGAAGGATGTTGAGGATCAGCGCATGTGGAAGAGGGAGGCGTCGAAAGCAAGACAAGAAACCAAGATAGGGTTCACGGCGAGAGTGGATGCCAAGATCAGACATTTGAGAGGGAAGCTACAAAAGAAGCATCATACCCCTGTAAGTACGCAAGGAGGGATGTCACCGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1228

Amino Acids

138.24

Weight (kDa)

6.52

Isoelectric Point (pI)

39.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	42 - 109	6e-25	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	539 - 734	1.2e-22	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	908 - 1158	1.5e-90	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	165
AarI	CACCTGC	1 cut(s)	733
Acc36I	ACCTGC	3 cut(s)	733, 1298, 1537
Acc65I	GGTACC	2 cut(s)	3185, 3297
AccB1I	GGYRCC	5 cut(s)	85, 104, 2206, 3185, 3297
AccBSI	CCGCTC	1 cut(s)	885
AccI	GTMKAC	1 cut(s)	1254
AccII	CGCG	2 cut(s)	1001, 1782
AccIII	TCCGGA	1 cut(s)	1799
AciI	CCGC	4 cut(s)	885, 1561, 1780, 2450
AclWI	GGATC	8 cut(s)	516, 686, 1255, 1480, 2155, 2441, 3486, 3534
AcoI	YGGCCR	1 cut(s)	3157
AcsI	RAATTY	9 cut(s)	178, 601, 856, 983, 1228, 1844, 2350, 3040, 3357
AcuI	CTGAAG	1 cut(s)	1331
AcyI	GRCGYC	3 cut(s)	86, 105, 3551
AfiI	CCNNNNNNNGG	8 cut(s)	101, 1539, 1567, 1570, 1603, 2754, 3277, 3579
AflII	CTTAAG	1 cut(s)	2243
AflIII	ACRYGT	4 cut(s)	999, 1445, 2358, 2919
AjnI	CCWGG	1 cut(s)	1248
AjuI	GAANNNNNNNTTGG	6 cut(s)	248, 280, 2667, 2699, 3566, 3598
AleI	CACNNNNGTG	1 cut(s)	3679
Alw26I	GTCTC	9 cut(s)	71, 356, 569, 1190, 1484, 2148, 2259, 2575, 2761
AlwI	GGATC	8 cut(s)	516, 686, 1255, 1480, 2155, 2441, 3486, 3534
Ama87I	CYCGRG	1 cut(s)	1652
Aor13HI	TCCGGA	1 cut(s)	1799
AoxI	GGCC	3 cut(s)	1063, 1246, 3157
ApeKI	GCWGC	9 cut(s)	275, 1367, 1370, 1709, 1724, 2456, 2562, 3176, 3325
ApoI	RAATTY	9 cut(s)	178, 601, 856, 983, 1228, 1844, 2350, 3040, 3357
ArsI	GACNNNNNNTTYG	2 cut(s)	1213, 1245
AseI	ATTAAT	1 cut(s)	2468
Asp700I	GAANNNNTTC	1 cut(s)	389
Asp718I	GGTACC	2 cut(s)	3185, 3297
AspLEI	GCGC	4 cut(s)	88, 107, 656, 3534
AspS9I	GGNCC	2 cut(s)	3032, 3377
AsuII	TTCGAA	1 cut(s)	785
AvaI	CYCGRG	1 cut(s)	1652
AvaII	GGWCC	2 cut(s)	3032, 3377
BaeGI	GKGCMC	1 cut(s)	2211
BaeI	ACNNNNGTAYC	2 cut(s)	3056, 3089
BalI	TGGCCA	1 cut(s)	3159
BanI	GGYRCC	5 cut(s)	85, 104, 2206, 3185, 3297
BbsI	GAAGAC	3 cut(s)	983, 1404, 3100
BbvI	GCAGC	9 cut(s)	262, 1379, 1382, 1721, 1736, 2468, 2549, 3163, 3312
BceAI	ACGGC	4 cut(s)	687, 956, 2550, 3603
BciT130I	CCWGG	1 cut(s)	1250
BciVI	GTATCC	2 cut(s)	759, 3057
BclI	TGATCA	3 cut(s)	951, 1077, 2430
BcoDI	GTCTC	9 cut(s)	71, 356, 569, 1190, 1484, 2148, 2259, 2575, 2761
BfaI	CTAG	6 cut(s)	1386, 1487, 1851, 2730, 3036, 3362
BfmI	CTRYAG	2 cut(s)	522, 2175
BfoI	RGCGCY	3 cut(s)	89, 108, 657
BfrI	CTTAAG	1 cut(s)	2243
BfuAI	ACCTGC	3 cut(s)	733, 1298, 1537
BfuI	GTATCC	2 cut(s)	759, 3057
BglII	AGATCT	2 cut(s)	2247, 2331
BmcAI	AGTACT	2 cut(s)	2060, 3506
Bme1390I	CCNGG	1 cut(s)	1250
Bme18I	GGWCC	2 cut(s)	3032, 3377
BmeT110I	CYCGRG	1 cut(s)	1652
BmgT120I	GGNCC	2 cut(s)	3032, 3377
BmiI	GGNNCC	5 cut(s)	87, 106, 2208, 3187, 3299
BmrFI	CCNGG	1 cut(s)	1250
BmrI	ACTGGG	4 cut(s)	1209, 2206, 2743, 3077
BmsI	GCATC	5 cut(s)	2558, 3213, 3274, 3589, 3649
BmuI	ACTGGG	4 cut(s)	1209, 2206, 2743, 3077
BpiI	GAAGAC	3 cut(s)	983, 1404, 3100
BplI	GAGNNNNNCTC	2 cut(s)	2794, 2826
BpmI	CTGGAG	1 cut(s)	297
Bpu10I	CCTNAGC	1 cut(s)	791
Bpu14I	TTCGAA	1 cut(s)	785
BpuEI	CTTGAG	6 cut(s)	100, 749, 796, 1197, 2066, 2147
Bsa29I	ATCGAT	1 cut(s)	2475
BsaAI	YACGTR	1 cut(s)	3255
BsaHI	GRCGYC	3 cut(s)	86, 105, 3551
BsaI	GGTCTC	3 cut(s)	71, 356, 569
BsaJI	CCNNGG	3 cut(s)	81, 100, 464
BsaWI	WCCGGW	1 cut(s)	1799
BsaXI	ACNNNNNCTCC	6 cut(s)	129, 159, 477, 507, 2042, 2072
Bsc4I	CCNNNNNNNGG	8 cut(s)	101, 1539, 1567, 1570, 1603, 2754, 3277, 3579
Bse118I	RCCGGY	1 cut(s)	107
Bse3DI	GCAATG	5 cut(s)	1320, 2841, 2948, 3088, 3297
BseAI	TCCGGA	1 cut(s)	1799
BseBI	CCWGG	1 cut(s)	1250
BseCI	ATCGAT	1 cut(s)	2475
BseDI	CCNNGG	3 cut(s)	81, 100, 464
BseLI	CCNNNNNNNGG	8 cut(s)	101, 1539, 1567, 1570, 1603, 2754, 3277, 3579
BseMI	GCAATG	5 cut(s)	1320, 2841, 2948, 3088, 3297
BseMII	CTCAG	5 cut(s)	367, 805, 2046, 2825, 2985
BseRI	GAGGAG	1 cut(s)	2063
BseSI	GKGCMC	1 cut(s)	2211
BseXI	GCAGC	9 cut(s)	262, 1379, 1382, 1721, 1736, 2468, 2549, 3163, 3312
BseYI	CCCAGC	1 cut(s)	2009
Bsh1236I	CGCG	2 cut(s)	1001, 1782
BshFI	GGCC	3 cut(s)	1065, 1248, 3159
BshNI	GGYRCC	5 cut(s)	85, 104, 2206, 3185, 3297
BshVI	ATCGAT	1 cut(s)	2475
BsiHKCI	CYCGRG	1 cut(s)	1652
BsiSI	CCGG	3 cut(s)	108, 1062, 1800
BslFI	GGGAC	2 cut(s)	853, 2379
BslI	CCNNNNNNNGG	8 cut(s)	101, 1539, 1567, 1570, 1603, 2754, 3277, 3579
BsmAI	GTCTC	9 cut(s)	71, 356, 569, 1190, 1484, 2148, 2259, 2575, 2761
BsmFI	GGGAC	2 cut(s)	853, 2379
BsmI	GAATGC	5 cut(s)	2127, 2876, 3327, 3352, 3452
BsnI	GGCC	3 cut(s)	1065, 1248, 3159
Bso31I	GGTCTC	3 cut(s)	71, 356, 569
BsoBI	CYCGRG	1 cut(s)	1652
Bsp119I	TTCGAA	1 cut(s)	785
Bsp1286I	GDGCHC	1 cut(s)	2211
Bsp13I	TCCGGA	1 cut(s)	1799
Bsp19I	CCATGG	1 cut(s)	464
BspACI	CCGC	4 cut(s)	885, 1561, 1780, 2450
BspANI	GGCC	3 cut(s)	1065, 1248, 3159
BspCNI	CTCAG	5 cut(s)	366, 804, 2047, 2824, 2986
BspDI	ATCGAT	1 cut(s)	2475
BspEI	TCCGGA	1 cut(s)	1799
BspFNI	CGCG	2 cut(s)	1001, 1782
BspLI	GGNNCC	5 cut(s)	87, 106, 2208, 3187, 3299
BspMAI	CTGCAG	1 cut(s)	526
BspMI	ACCTGC	3 cut(s)	733, 1298, 1537
BspPI	GGATC	8 cut(s)	516, 686, 1255, 1480, 2155, 2441, 3486, 3534
BspT104I	TTCGAA	1 cut(s)	785
BspT107I	GGYRCC	5 cut(s)	85, 104, 2206, 3185, 3297
BspTI	CTTAAG	1 cut(s)	2243
BspTNI	GGTCTC	3 cut(s)	71, 356, 569
BsrBI	CCGCTC	1 cut(s)	885
BsrDI	GCAATG	5 cut(s)	1320, 2841, 2948, 3088, 3297
BsrFI	RCCGGY	1 cut(s)	107
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	3 cut(s)	81, 100, 464
BssNI	GRCGYC	3 cut(s)	86, 105, 3551
BssT1I	CCWWGG	2 cut(s)	100, 464
Bst2UI	CCWGG	1 cut(s)	1250
Bst4CI	ACNGT	7 cut(s)	124, 173, 1495, 2546, 2648, 3009, 3678
Bst6I	CTCTTC	5 cut(s)	147, 1188, 1233, 2867, 3536
BstACI	GRCGYC	3 cut(s)	86, 105, 3551
BstAFI	CTTAAG	1 cut(s)	2243
BstAPI	GCANNNNNTGC	1 cut(s)	2462
BstBAI	YACGTR	1 cut(s)	3255
BstBI	TTCGAA	1 cut(s)	785
BstC8I	GCNNGC	8 cut(s)	109, 992, 1053, 1155, 1159, 1416, 2454, 2718
BstDSI	CCRYGG	1 cut(s)	464
BstFNI	CGCG	2 cut(s)	1001, 1782
BstH2I	RGCGCY	3 cut(s)	89, 108, 657
BstHHI	GCGC	4 cut(s)	88, 107, 656, 3534
BstMAI	GTCTC	9 cut(s)	71, 356, 569, 1190, 1484, 2148, 2259, 2575, 2761
BstMWI	GCNNNNNNNGC	8 cut(s)	368, 963, 1331, 2462, 2726, 3149, 3173, 3557
BstNI	CCWGG	1 cut(s)	1250
BstNSI	RCATGY	6 cut(s)	994, 2304, 2456, 2923, 3426, 3538
BstSCI	CCNGG	1 cut(s)	1248
BstSFI	CTRYAG	2 cut(s)	522, 2175
BstSLI	GKGCMC	1 cut(s)	2211
BstUI	CGCG	2 cut(s)	1001, 1782
BstV1I	GCAGC	9 cut(s)	262, 1379, 1382, 1721, 1736, 2468, 2549, 3163, 3312
BstV2I	GAAGAC	3 cut(s)	983, 1404, 3100
BstX2I	RGATCY	4 cut(s)	691, 2247, 2331, 2446
BstXI	CCANNNNNNTGG	2 cut(s)	1002, 3393
BstYI	RGATCY	4 cut(s)	691, 2247, 2331, 2446
Bsu15I	ATCGAT	1 cut(s)	2475
BsuI	GTATCC	2 cut(s)	759, 3057
BsuRI	GGCC	3 cut(s)	1065, 1248, 3159
BsuTUI	ATCGAT	1 cut(s)	2475
BtgI	CCRYGG	1 cut(s)	464
BtgZI	GCGATG	1 cut(s)	386
BtsI	GCAGTG	3 cut(s)	1080, 1641, 2080
BveI	ACCTGC	3 cut(s)	733, 1298, 1537
Cac8I	GCNNGC	8 cut(s)	109, 992, 1053, 1155, 1159, 1416, 2454, 2718
CfoI	GCGC	4 cut(s)	88, 107, 656, 3534
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	2 cut(s)	3032, 3377
ClaI	ATCGAT	1 cut(s)	2475
CseI	GACGC	2 cut(s)	1007, 3540
DinI	GGCGCC	2 cut(s)	87, 106
EaeI	YGGCCR	1 cut(s)	3157
Eam1104I	CTCTTC	5 cut(s)	147, 1188, 1233, 2867, 3536
EarI	CTCTTC	5 cut(s)	147, 1188, 1233, 2867, 3536
Eco130I	CCWWGG	2 cut(s)	100, 464
Eco31I	GGTCTC	3 cut(s)	71, 356, 569
Eco32I	GATATC	1 cut(s)	1103
Eco47I	GGWCC	2 cut(s)	3032, 3377
Eco57I	CTGAAG	1 cut(s)	1331
Eco88I	CYCGRG	1 cut(s)	1652
EcoO109I	RGGNCCY	1 cut(s)	3032
EcoRI	GAATTC	2 cut(s)	856, 1844
EcoRII	CCWGG	1 cut(s)	1248
EcoRV	GATATC	1 cut(s)	1103
EcoT14I	CCWWGG	2 cut(s)	100, 464
EgeI	GGCGCC	2 cut(s)	87, 106
EheI	GGCGCC	2 cut(s)	87, 106
ErhI	CCWWGG	2 cut(s)	100, 464
FalI	AAGNNNNNCTT	8 cut(s)	1040, 1072, 1347, 1379, 2226, 2258, 2482, 2514
FaqI	GGGAC	2 cut(s)	853, 2379
FauNDI	CATATG	3 cut(s)	1336, 1684, 2558
FbaI	TGATCA	3 cut(s)	951, 1077, 2430
FblI	GTMKAC	1 cut(s)	1254
FspBI	CTAG	6 cut(s)	1386, 1487, 1851, 2730, 3036, 3362
GlaI	GCGC	4 cut(s)	87, 106, 655, 3533
GsaI	CCCAGC	1 cut(s)	2013
GsuI	CTGGAG	1 cut(s)	297
HaeII	RGCGCY	3 cut(s)	89, 108, 657
HaeIII	GGCC	3 cut(s)	1065, 1248, 3159
HapII	CCGG	3 cut(s)	108, 1062, 1800
HgaI	GACGC	2 cut(s)	1007, 3540
HhaI	GCGC	4 cut(s)	88, 107, 656, 3534
Hin1I	GRCGYC	3 cut(s)	86, 105, 3551
Hin6I	GCGC	4 cut(s)	86, 105, 654, 3532
HinP1I	GCGC	4 cut(s)	86, 105, 654, 3532
HincII	GTYRAC	3 cut(s)	1287, 2542, 3220
HindII	GTYRAC	3 cut(s)	1287, 2542, 3220
HindIII	AAGCTT	3 cut(s)	26, 1159, 2042
HinfI	GANTC	8 cut(s)	782, 1204, 1589, 1694, 2051, 2668, 2694, 3259
HpaI	GTTAAC	2 cut(s)	1287, 3220
HpaII	CCGG	3 cut(s)	108, 1062, 1800
Hpy166II	GTNNAC	7 cut(s)	1255, 1287, 2542, 2782, 3220, 3445, 3585
Hpy188III	TCNNGA	9 cut(s)	117, 728, 775, 793, 836, 1652, 1800, 2672, 3681
Hpy8I	GTNNAC	7 cut(s)	1255, 1287, 2542, 2782, 3220, 3445, 3585
Hpy99I	CGWCG	3 cut(s)	420, 3556, 3682
HpyAV	CCTTC	8 cut(s)	223, 724, 882, 1138, 2578, 2702, 3154, 3508
HpyCH4III	ACNGT	7 cut(s)	124, 173, 1495, 2546, 2648, 3009, 3678
HpyCH4IV	ACGT	7 cut(s)	1283, 1438, 1447, 1502, 2360, 2658, 3254
HpyF10VI	GCNNNNNNNGC	8 cut(s)	368, 963, 1331, 2462, 2726, 3149, 3173, 3557
HpySE526I	ACGT	7 cut(s)	1283, 1438, 1447, 1502, 2360, 2658, 3254
Hsp92I	GRCGYC	3 cut(s)	86, 105, 3551
HspAI	GCGC	4 cut(s)	86, 105, 654, 3532
KasI	GGCGCC	2 cut(s)	85, 104
Kpn2I	TCCGGA	1 cut(s)	1799
KpnI	GGTACC	2 cut(s)	3189, 3301
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
Ksp22I	TGATCA	3 cut(s)	951, 1077, 2430
KspAI	GTTAAC	2 cut(s)	1287, 3220
LmnI	GCTCC	5 cut(s)	334, 882, 2070, 2316, 3344
Lsp1109I	GCAGC	9 cut(s)	262, 1379, 1382, 1721, 1736, 2468, 2549, 3163, 3312
LweI	GCATC	5 cut(s)	2558, 3213, 3274, 3589, 3649
MaeI	CTAG	6 cut(s)	1386, 1487, 1851, 2730, 3036, 3362
MaeII	ACGT	7 cut(s)	1283, 1438, 1447, 1502, 2360, 2658, 3254
MbiI	CCGCTC	1 cut(s)	885
MfeI	CAATTG	1 cut(s)	1131
MflI	RGATCY	4 cut(s)	691, 2247, 2331, 2446
MhlI	GDGCHC	1 cut(s)	2211
MlsI	TGGCCA	1 cut(s)	3159
MluI	ACGCGT	1 cut(s)	999
MluNI	TGGCCA	1 cut(s)	3159
Mly113I	GGCGCC	2 cut(s)	86, 105
MlyI	GAGTC	3 cut(s)	1598, 1703, 2060
MmeI	TCCRAC	6 cut(s)	463, 2338, 2418, 2641, 2671, 3356
Mox20I	TGGCCA	1 cut(s)	3159
MroI	TCCGGA	1 cut(s)	1799
MroNI	GCCGGC	1 cut(s)	107
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	1 cut(s)	3159
MslI	CAYNNNNRTG	3 cut(s)	1407, 1992, 3679
Msp20I	TGGCCA	1 cut(s)	3159
MspA1I	CMGCKG	2 cut(s)	2180, 3167
MspCI	CTTAAG	1 cut(s)	2243
MspI	CCGG	3 cut(s)	108, 1062, 1800
MspR9I	CCNGG	1 cut(s)	1250
MunI	CAATTG	1 cut(s)	1131
Mva1269I	GAATGC	5 cut(s)	2127, 2876, 3327, 3352, 3452
MvaI	CCWGG	1 cut(s)	1250
MvnI	CGCG	2 cut(s)	1001, 1782
MwoI	GCNNNNNNNGC	8 cut(s)	368, 963, 1331, 2462, 2726, 3149, 3173, 3557
NaeI	GCCGGC	1 cut(s)	109
NarI	GGCGCC	2 cut(s)	86, 105
NcoI	CCATGG	1 cut(s)	464
NdeI	CATATG	3 cut(s)	1336, 1684, 2558
NgoMIV	GCCGGC	1 cut(s)	107
NlaIV	GGNNCC	5 cut(s)	87, 106, 2208, 3187, 3299
NmuCI	GTSAC	6 cut(s)	833, 1388, 1695, 2642, 2732, 3672
NspI	RCATGY	6 cut(s)	994, 2304, 2456, 2923, 3426, 3538
NspV	TTCGAA	1 cut(s)	785
OliI	CACNNNNGTG	1 cut(s)	3679
PaeI	GCATGC	2 cut(s)	994, 2456
PaqCI	CACCTGC	1 cut(s)	733
PciI	ACATGT	1 cut(s)	2919
PcsI	WCGNNNNNNNCGW	1 cut(s)	1444
PctI	GAATGC	5 cut(s)	2127, 2876, 3327, 3352, 3452
PdiI	GCCGGC	1 cut(s)	109
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	5 cut(s)	782, 1204, 2668, 2694, 3259
PleI	GAGTC	3 cut(s)	1597, 1702, 2059
PluTI	GGCGCC	2 cut(s)	89, 108
PpsI	GAGTC	3 cut(s)	1597, 1702, 2059
Ppu21I	YACGTR	1 cut(s)	3255
PpuMI	RGGWCCY	1 cut(s)	3032
PscI	ACATGT	1 cut(s)	2919
PshBI	ATTAAT	1 cut(s)	2468
PsiI	TTATAA	1 cut(s)	165
Psp5II	RGGWCCY	1 cut(s)	3032
Psp6I	CCWGG	1 cut(s)	1248
PspFI	CCCAGC	1 cut(s)	2009
PspGI	CCWGG	1 cut(s)	1248
PspN4I	GGNNCC	5 cut(s)	87, 106, 2208, 3187, 3299
PspPI	GGNCC	2 cut(s)	3032, 3377
PspPPI	RGGWCCY	1 cut(s)	3032
PstI	CTGCAG	1 cut(s)	526
PsuI	RGATCY	4 cut(s)	691, 2247, 2331, 2446
PvuII	CAGCTG	2 cut(s)	2180, 3167
RseI	CAYNNNNRTG	3 cut(s)	1407, 1992, 3679
Sau96I	GGNCC	2 cut(s)	3032, 3377
ScaI	AGTACT	2 cut(s)	2060, 3506
SchI	GAGTC	3 cut(s)	1598, 1703, 2060
ScrFI	CCNGG	1 cut(s)	1250
SduI	GDGCHC	1 cut(s)	2211
SfaNI	GCATC	5 cut(s)	2558, 3213, 3274, 3589, 3649
SfcI	CTRYAG	2 cut(s)	522, 2175
SfoI	GGCGCC	2 cut(s)	87, 106
SfuI	TTCGAA	1 cut(s)	785
SinI	GGWCC	2 cut(s)	3032, 3377
SmiMI	CAYNNNNRTG	3 cut(s)	1407, 1992, 3679
SmlI	CTYRAG	7 cut(s)	115, 728, 775, 1212, 2045, 2126, 2243
SmoI	CTYRAG	7 cut(s)	115, 728, 775, 1212, 2045, 2126, 2243
SphI	GCATGC	2 cut(s)	994, 2456
SsiI	CCGC	4 cut(s)	885, 1561, 1780, 2450
SspDI	GGCGCC	2 cut(s)	85, 104
SspMI	CTAG	6 cut(s)	1386, 1487, 1851, 2730, 3036, 3362
StyD4I	CCNGG	1 cut(s)	1248
StyI	CCWWGG	2 cut(s)	100, 464
TaaI	ACNGT	7 cut(s)	124, 173, 1495, 2546, 2648, 3009, 3678
TaiI	ACGT	7 cut(s)	1286, 1441, 1450, 1505, 2363, 2661, 3257
TaqI	TCGA	7 cut(s)	785, 1942, 2475, 2706, 2871, 3366, 3554
TaqII	GACCGA	1 cut(s)	95
TatI	WGTACW	4 cut(s)	1821, 2058, 2779, 3504
TauI	GCSGC	1 cut(s)	1563
TfiI	GAWTC	5 cut(s)	782, 1204, 2668, 2694, 3259
TseFI	GTSAC	6 cut(s)	833, 1388, 1695, 2642, 2732, 3672
TseI	GCWGC	9 cut(s)	275, 1367, 1370, 1709, 1724, 2456, 2562, 3176, 3325
Tsp45I	GTSAC	6 cut(s)	833, 1388, 1695, 2642, 2732, 3672
TspGWI	ACGGA	6 cut(s)	1043, 1234, 1334, 1636, 3137, 3333
Vha464I	CTTAAG	1 cut(s)	2243
VpaK11BI	GGWCC	2 cut(s)	3032, 3377
VspI	ATTAAT	1 cut(s)	2468
XapI	RAATTY	9 cut(s)	178, 601, 856, 983, 1228, 1844, 2350, 3040, 3357
XceI	RCATGY	6 cut(s)	994, 2304, 2456, 2923, 3426, 3538
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1600
XmiI	GTMKAC	1 cut(s)	1254
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	6 cut(s)	1386, 1487, 1851, 2730, 3036, 3362
ZrmI	AGTACT	2 cut(s)	2060, 3506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.