Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Forward (+)

58848813 .. 58854463

Gene Structure 5651 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G035770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3597 bp

ATGGCTGGAGGAAGAATAAGGGCAAGGCTACGGCGGAGCCACCTCTACACATTCTTGCGTAAGCCAAGGGGCAATGAGTCAGGGGCACCTCATCCAATCCAAGGTCCTGGGTACTCGAGAACTGTACATTGTAACCAACCTCTGCTCCATCAAAAGAAACCTTACAAATACCGTTCAAATTACATATCGACCACAAAGTATAACCCGATCACCTTCTTGCCCAAGGCACTCTTTGAGCAGTTCAGACGGGTTGCCAATATCTATTTCCTTTTGGCTGCCGGTCTTTCACTCACTTCTGTTTCTCCATTCGGTCCTTGGAGCATGATAGCTCCTTTGGCTTTTGTTGTTGGTCTCAGTATGGCAAAGGAAGCTATGGAAGACTGGAATAGGTTCTTGCAGGACATGAAGGTTAATCTTCGGAAAGTAAATGTTCATAAAGGAGATGGTGTTTTCGGATATAGGCCATGGCATAAGATTGTGGTTGGAGATGTGGTGAAAGTGGAAAAGGATAAATTCTTTCCTGCTGATCTGCTTCTATTGTCGTCAAGTTATGAGGATGGCTTCTGTTATGTTGAGACTATGAACTTAGATGGCGAGACAAATTTGAAGGTCAAGAGATGCTTGGAGGTAACCTCACCTTTGGATGACGATGGAGTTTTCAAGGACTTCACAGCAACAGTTCAATGTGAAGATCCAAATCCCAATCTTTATTCCTTTGTGGGTAATCTTGATCATGACCGGCAGATTTATCCTCTTGAACCCGGTCAGATACTCCTGAGAGATTCAAAGCTCAGGAATACAAGTTATATCTATGGGGTGGTGATATTTACAGGTCATGATAGCAAAGTCATGCAGAATTCGACAAAGTCCCCCTCAAAAAGGAGTGGAATAGAAAAGAAAATGGACAAGATTATCTACATCCTTTTCTTCATCCTTTTAGTGATCTCCTTGATCAGCTCAATAGGGTTTGGTGTGAAGACAAGAAAAGATATGCCATCGGCATGGTACTTACGACCAGACGATGCAGAAGATATGTATAGCCCAAAAAAACCTGCCGCAGCTGGCGCTATCCATCTGGTTGTGAAGGTCTTGCAAGCAAAGTTCATTAACAATGACATACACATGTATGATGAAGAGCATGGAACTCCTGCTCAAGCCCGGACATCAAATTTGAATGAGGAGTTAGGCCAGGTAGACACGATCCTTTCTGACAAAACTGGCACTTTAACCTGCAACCAGATGGACTTTCTGAAGTGTTGCATTGCTGGCATTGCATACGGTGTCCGCTCTAGTGAAGTTGAACTTGCAGCAGCAAAACAGATGGCTACTGACCTTGATGATCAGGACGAGCATGATGAAGAACTTGCCAATGTTCCCAGGAATAAACATAGTCAACAAGTATCATGGGGGAATGAAATAGGATCAGAAATTGAGCTGGAAACTGTTGTTACATCCAAGGATGGTAAGGATCCGAAACCTGCCATAAAAGGGTTTAGCTTTGTGGACAACCGCCTCATGGATGGAAATTGGTTGAGAGAGCCCACCTGTGATGTGATTTTATTATTTTTCCGGATTTTAGCCGTTTGCCATACTGCAATTCCTGAGCTAAATGAAGAGACTGGCCAATATACATATGAAGCAGAGTCACCCGATGAAGGAGCTTTTCTTGTTGCAGCACGTGAGTTAGGTTTTGAGTTCTGTAAAAGAAATCAGTCAAGTGTGTATGTCCGCGAAAAGTATCCTGATCCAGGACAACCAGTGGAAAGGGAGTATCAAGTCTTAAATCTATTGGAATTCACCAGCAAAAGGAAGCGTATGTCAGTAATTGTGCGTGACGAAGATGGGCAATTATTACTTTTCTGCAAAGGTGCTGACAGCATCATTTTCGATCGACTGTCAAAGAATGGAAGAATTTATGAGGAAGCTAGTACCAAGCATTTGAATGAATATGGAGAAGCTGGGTTGCGTACGTTGGCACTTGCTTATAAAAAGCTTGAGGAGTCCGAGTATTCTGCTTGGAACAATGAATTTCAGAAAGCAAAATCATCTATCGGGGCTGATAGGGAGGTGATGCTCGAGCGAGTAGCAGAGAAGATGGAAAAAGACTTGATCATGGTTGGTGCTACAGCTGTGCCCCAATGCATAGACAATCTTGCACAAGCTGGTCTCAAGATCTGGGTGTTGACAGGGGATAAGATGGAAACTGCAATCAACATAGGATTTGCATGTAGTTTGCTCCGACAGGGCATGAAGCAGATCTGTATATCAACAGCAAATTTAGACACATTGGGACAAGATGGAAAAGAGGCTGTGAAAGACAACATTTTGAATCAAATTACGAATGCCTCTCAAATGATCAAGTTGGAAACAGATCCACATGCAGCGTTTGCACTAATTATTGATGGAAAAACTTTAACCTATGCTTTGGACGCTGATATGAAGCATCTATTCTTGGAATTAGCTGTTGATTGTGCATCTGTCATATGCTGTCGTGTCTCCCCTAAGCAGAAGGCATTGGTAACGAGGTTAGTAAGACAAGGAACTGGGAAGACTACATTAGCAATTGGTGATGGTGCAAATGATGTTGGAATGATTCAAGAAGCTGATATTGGTGTTGGAATCAGTGGTGTCGAAGGTATGCAGGCTGTGATGGCTAGTGACTTTGCTATTGCCCAGTTTCGGTTTTTGGAGAGACTTTTGGTTGTCCATGGACATTGGTGCTATAAAAGGATTGCTCAGATGATTTGCTATTTCTTCTACAAAAATATAGCATTTGGTCTGACCCTCTTCTACTTTGAGGCATTCACGGGATTTTCGGGGCAATCAATATATGCTGACTTGTACATGCTAACGTTCAATGTTATTCTTACCTCATTGCCTGTCATATCACTTGGAGTATTTGAACAAGATGTTTCTTCCGAGGTCTGCTTACAGTTCCCTGCATTATATCAGCAAGGAGCCCGAAATTTGTTCTTCGACTGGTACAGAATACTGGGGTGGATGGGCAATGGTGTATATTGCTCTCTCATTGTCTTCTTCCTCAACATTATCATATTCAAAGACCAGTCCTTCAGGTCTGATGGCCAGATTGCTGATATGGCTGCTATGGGTACAACTATGTTCAGTTGCATCATATGGGCCGTCAACTGCCAGATTGCTCTGGCAATGTGTCACTTTACATGGATTCAACACTGCTTTATTTGGGGCAGTATTGCAATGTGGTACCTATTTCTCTTAATTTACGGCATAGTGACACCAACGTGGTCTGGAAATGCCTACCAGATTCTAGTTGAAGTCCTCGGTCCTGCACCCATCTTTTGGTCAGCCACCCTATTAGTTACTCTTGCTTGTAATCTCCCTTACATTGTCCACATAGCCTTCCAAAGAAGTTTCAATCCAATGGATCATCATATCATCCAAGAAATCAAATACTACCGGAAAGATGTTCAGGATCAACGCATGTGGAAGAGGGAGGCATCTAAAGCAAGACAAGAAACCAAGATTGGGTTCTCAGCTCGGGTAGATGCAAAGATCAGACAATTGAGAGCAAGGCTGCACAAGAAGCAACCCCAGAGTAACGCAATGTCCCCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1198

Amino Acids

135.21

Weight (kDa)

6.7

Isoelectric Point (pI)

38.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	42 - 109	7.3e-24	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	523 - 710	8.7e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	883 - 1133	8.7e-88	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1986
Acc36I	ACCTGC	3 cut(s)	1060, 1238, 1486
Acc65I	GGTACC	1 cut(s)	3222
AccB1I	GGYRCC	2 cut(s)	85, 3222
AccB7I	CCANNNNNTGG	1 cut(s)	3318
AccBSI	CCGCTC	1 cut(s)	1287
AccI	GTMKAC	1 cut(s)	1194
AccII	CGCG	1 cut(s)	1731
AccIII	TCCGGA	1 cut(s)	1569
AciI	CCGC	5 cut(s)	34, 1056, 1285, 1510, 1729
AclI	AACGTT	1 cut(s)	2852
AclWI	GGATC	9 cut(s)	686, 1195, 1429, 1463, 1476, 1739, 2366, 3411, 3459
AcoI	YGGCCR	2 cut(s)	1621, 3082
AcsI	RAATTY	9 cut(s)	512, 601, 856, 1168, 1793, 1911, 2027, 2275, 2965
AcuI	CTGAAG	2 cut(s)	1271, 3055
AcvI	CACGTG	1 cut(s)	1679
AfaI	GTAC	9 cut(s)	113, 126, 1007, 1930, 1969, 2843, 2984, 3112, 3224
AflIII	ACRYGT	1 cut(s)	1122
AjnI	CCWGG	4 cut(s)	106, 1188, 1376, 1747
AjuI	GAANNNNNNNTTGG	8 cut(s)	248, 280, 2592, 2624, 3486, 3491, 3518, 3523
AleI	CACNNNNGTG	1 cut(s)	3259
Alw26I	GTCTC	7 cut(s)	356, 569, 590, 1610, 2171, 2500, 2686
AlwI	GGATC	9 cut(s)	686, 1195, 1429, 1463, 1476, 1739, 2366, 3411, 3459
Ama87I	CYCGRG	3 cut(s)	115, 2075, 3516
Aor13HI	TCCGGA	1 cut(s)	1569
AoxI	GGCC	5 cut(s)	461, 1186, 1621, 3082, 3138
ApeKI	GCWGC	8 cut(s)	275, 1058, 1307, 1310, 1673, 2381, 3101, 3553
ApoI	RAATTY	9 cut(s)	512, 601, 856, 1168, 1793, 1911, 2027, 2275, 2965
ArsI	GACNNNNNNTTYG	2 cut(s)	1153, 1185
Asp700I	GAANNNNTTC	1 cut(s)	389
Asp718I	GGTACC	1 cut(s)	3222
AspLEI	GCGC	1 cut(s)	1067
AspS9I	GGNCC	4 cut(s)	104, 311, 3138, 3302
AsuC2I	CCSGG	2 cut(s)	762, 1159
AsuHPI	GGTGA	8 cut(s)	202, 505, 627, 832, 1638, 1789, 2080, 2579
AvaI	CYCGRG	3 cut(s)	115, 2075, 3516
AvaII	GGWCC	3 cut(s)	104, 311, 3302
BaeGI	GKGCMC	2 cut(s)	88, 2136
BalI	TGGCCA	2 cut(s)	1623, 3084
BamHI	GGATCC	1 cut(s)	1468
BanI	GGYRCC	2 cut(s)	85, 3222
BanII	GRGCYC	2 cut(s)	1542, 2962
BbrPI	CACGTG	1 cut(s)	1679
BbsI	GAAGAC	4 cut(s)	384, 983, 2555, 3025
BbvI	GCAGC	8 cut(s)	262, 1070, 1319, 1322, 1685, 2393, 3088, 3540
BceAI	ACGGC	4 cut(s)	47, 1565, 3125, 3259
BcgI	CGANNNNNNTGC	4 cut(s)	1867, 1901, 1994, 2028
BciT130I	CCWGG	4 cut(s)	108, 1190, 1378, 1749
BciVI	GTATCC	1 cut(s)	1749
BclI	TGATCA	5 cut(s)	730, 951, 1339, 2109, 2355
BcnI	CCSGG	2 cut(s)	762, 1159
BcoDI	GTCTC	7 cut(s)	356, 569, 590, 1610, 2171, 2500, 2686
BfaI	CTAG	4 cut(s)	1290, 1926, 2655, 3287
BfmI	CTRYAG	1 cut(s)	2124
BfoI	RGCGCY	1 cut(s)	1068
BfuAI	ACCTGC	3 cut(s)	1060, 1238, 1486
BfuI	GTATCC	1 cut(s)	1749
BglII	AGATCT	2 cut(s)	2172, 2256
BisI	GCNGC	9 cut(s)	276, 1056, 1059, 1308, 1311, 1674, 2382, 3102, 3554
BlsI	GCNGC	9 cut(s)	277, 1057, 1060, 1309, 1312, 1675, 2383, 3103, 3555
Bme1390I	CCNGG	6 cut(s)	108, 762, 1159, 1190, 1378, 1749
Bme18I	GGWCC	3 cut(s)	104, 311, 3302
BmeT110I	CYCGRG	3 cut(s)	115, 2075, 3516
BmgT120I	GGNCC	4 cut(s)	104, 311, 3138, 3302
BmiI	GGNNCC	5 cut(s)	38, 87, 1470, 2959, 3224
BmrFI	CCNGG	6 cut(s)	108, 762, 1159, 1190, 1378, 1749
BmrI	ACTGGG	3 cut(s)	2553, 2668, 3002
BmsI	GCATC	9 cut(s)	608, 1012, 1887, 2061, 2452, 2483, 3138, 3485, 3514
BmuI	ACTGGG	3 cut(s)	2553, 2668, 3002
BpiI	GAAGAC	4 cut(s)	384, 983, 2555, 3025
BplI	GAGNNNNNCTC	2 cut(s)	617, 649
BpmI	CTGGAG	1 cut(s)	27
Bpu10I	CCTNAGC	3 cut(s)	791, 1602, 2502
BpuEI	CTTGAG	3 cut(s)	1137, 2015, 2153
BpuMI	CCSGG	2 cut(s)	762, 1159
BsaAI	YACGTR	1 cut(s)	1679
BsaBI	GATNNNNATC	1 cut(s)	696
BsaI	GGTCTC	2 cut(s)	356, 2171
BsaWI	WCCGGW	2 cut(s)	1569, 3435
BsaXI	ACNNNNNCTCC	8 cut(s)	129, 159, 432, 462, 477, 507, 1991, 2021
Bse118I	RCCGGY	2 cut(s)	278, 738
Bse1I	ACTGG	9 cut(s)	386, 1222, 1624, 1757, 2548, 2674, 2984, 2997, 3064
Bse3DI	GCAATG	8 cut(s)	79, 1260, 1269, 2873, 3013, 3171, 3222, 3588
Bse8I	GATNNNNATC	1 cut(s)	696
BseAI	TCCGGA	1 cut(s)	1569
BseBI	CCWGG	4 cut(s)	108, 1190, 1378, 1749
BseJI	GATNNNNATC	1 cut(s)	696
BseMI	GCAATG	8 cut(s)	79, 1260, 1269, 2873, 3013, 3171, 3222, 3588
BseMII	CTCAG	6 cut(s)	367, 767, 805, 1593, 2750, 3525
BseNI	ACTGG	9 cut(s)	386, 1222, 1624, 1757, 2548, 2674, 2984, 2997, 3064
BseRI	GAGGAG	2 cut(s)	1193, 2012
BseSI	GKGCMC	2 cut(s)	88, 2136
BseXI	GCAGC	8 cut(s)	262, 1070, 1319, 1322, 1685, 2393, 3088, 3540
BseYI	CCCAGC	1 cut(s)	1958
BsgI	GTGCAG	2 cut(s)	3291, 3539
Bsh1236I	CGCG	1 cut(s)	1731
Bsh1285I	CGRYCG	1 cut(s)	1891
BshFI	GGCC	5 cut(s)	463, 1188, 1623, 3084, 3140
BshNI	GGYRCC	2 cut(s)	85, 3222
BsiEI	CGRYCG	1 cut(s)	1891
BsiHKCI	CYCGRG	3 cut(s)	115, 2075, 3516
BsiSI	CCGG	6 cut(s)	279, 739, 762, 1159, 1570, 3436
BsiWI	CGTACG	1 cut(s)	1967
BslFI	GGGAC	3 cut(s)	853, 2304, 3571
BsmAI	GTCTC	7 cut(s)	356, 569, 590, 1610, 2171, 2500, 2686
BsmFI	GGGAC	3 cut(s)	853, 2304, 3571
BsmI	GAATGC	2 cut(s)	2347, 2801
BsnI	GGCC	5 cut(s)	463, 1188, 1623, 3084, 3140
Bso31I	GGTCTC	2 cut(s)	356, 2171
BsoBI	CYCGRG	3 cut(s)	115, 2075, 3516
Bsp1286I	GDGCHC	4 cut(s)	88, 1542, 2136, 2962
Bsp13I	TCCGGA	1 cut(s)	1569
Bsp1407I	TGTACA	2 cut(s)	124, 2841
Bsp19I	CCATGG	2 cut(s)	464, 2707
BspACI	CCGC	5 cut(s)	34, 1056, 1285, 1510, 1729
BspANI	GGCC	5 cut(s)	463, 1188, 1623, 3084, 3140
BspCNI	CTCAG	6 cut(s)	366, 768, 804, 1594, 2749, 3524
BspEI	TCCGGA	1 cut(s)	1569
BspFNI	CGCG	1 cut(s)	1731
BspHI	TCATGA	2 cut(s)	733, 835
BspLI	GGNNCC	5 cut(s)	38, 87, 1470, 2959, 3224
BspMI	ACCTGC	3 cut(s)	1060, 1238, 1486
BspPI	GGATC	9 cut(s)	686, 1195, 1429, 1463, 1476, 1739, 2366, 3411, 3459
BspQI	GCTCTTC	1 cut(s)	1128
BspT107I	GGYRCC	2 cut(s)	85, 3222
BspTNI	GGTCTC	2 cut(s)	356, 2171
BsrBI	CCGCTC	1 cut(s)	1287
BsrDI	GCAATG	8 cut(s)	79, 1260, 1269, 2873, 3013, 3171, 3222, 3588
BsrFI	RCCGGY	2 cut(s)	278, 738
BsrGI	TGTACA	2 cut(s)	124, 2841
BsrI	ACTGG	9 cut(s)	386, 1222, 1624, 1757, 2548, 2674, 2984, 2997, 3064
BssAI	RCCGGY	2 cut(s)	278, 738
BssT1I	CCWWGG	7 cut(s)	65, 100, 222, 314, 464, 1455, 2707
Bst2UI	CCWGG	4 cut(s)	108, 1190, 1378, 1749
Bst4CI	ACNGT	7 cut(s)	124, 173, 679, 1280, 1444, 1896, 2934
Bst6I	CTCTTC	4 cut(s)	1128, 1608, 2792, 3461
BstAPI	GCANNNNNTGC	1 cut(s)	2387
BstAUI	TGTACA	2 cut(s)	124, 2841
BstBAI	YACGTR	1 cut(s)	1679
BstC8I	GCNNGC	4 cut(s)	1063, 1095, 1267, 2643
BstDEI	CTNAG	8 cut(s)	353, 586, 776, 791, 1602, 2502, 2736, 3511
BstDSI	CCRYGG	2 cut(s)	464, 2707
BstEII	GGTNACC	1 cut(s)	628
BstENI	CCTNNNNNAGG	2 cut(s)	877, 1746
BstFNI	CGCG	1 cut(s)	1731
BstH2I	RGCGCY	1 cut(s)	1068
BstHHI	GCGC	1 cut(s)	1067
BstMAI	GTCTC	7 cut(s)	356, 569, 590, 1610, 2171, 2500, 2686
BstMCI	CGRYCG	1 cut(s)	1891
BstNI	CCWGG	4 cut(s)	108, 1190, 1378, 1749
BstNSI	RCATGY	5 cut(s)	1126, 2229, 2381, 2848, 3463
BstPI	GGTNACC	1 cut(s)	628
BstSCI	CCNGG	6 cut(s)	106, 760, 1157, 1188, 1376, 1747
BstSFI	CTRYAG	1 cut(s)	2124
BstSLI	GKGCMC	2 cut(s)	88, 2136
BstUI	CGCG	1 cut(s)	1731
BstV1I	GCAGC	8 cut(s)	262, 1070, 1319, 1322, 1685, 2393, 3088, 3540
BstV2I	GAAGAC	4 cut(s)	384, 983, 2555, 3025
BstX2I	RGATCY	5 cut(s)	691, 1468, 2172, 2256, 2371
BstXI	CCANNNNNNTGG	2 cut(s)	107, 1002
BstYI	RGATCY	5 cut(s)	691, 1468, 2172, 2256, 2371
BsuI	GTATCC	1 cut(s)	1749
BsuRI	GGCC	5 cut(s)	463, 1188, 1623, 3084, 3140
BtgI	CCRYGG	2 cut(s)	464, 2707
BtsI	GCAGTG	1 cut(s)	3190
BtsIMutI	CAGTG	3 cut(s)	1764, 2629, 3190
BveI	ACCTGC	3 cut(s)	1060, 1238, 1486
Cac8I	GCNNGC	4 cut(s)	1063, 1095, 1267, 2643
CciI	TCATGA	2 cut(s)	733, 835
CfoI	GCGC	1 cut(s)	1067
Cfr10I	RCCGGY	2 cut(s)	278, 738
Cfr13I	GGNCC	4 cut(s)	104, 311, 3138, 3302
CseI	GACGC	1 cut(s)	2438
Csp6I	GTAC	9 cut(s)	112, 125, 1006, 1929, 1968, 2842, 2983, 3111, 3223
CspCI	CAANNNNNGTGG	2 cut(s)	3443, 3478
CviQI	GTAC	9 cut(s)	112, 125, 1006, 1929, 1968, 2842, 2983, 3111, 3223
DdeI	CTNAG	8 cut(s)	353, 586, 776, 791, 1602, 2502, 2736, 3511
EaeI	YGGCCR	2 cut(s)	1621, 3082
Eam1104I	CTCTTC	4 cut(s)	1128, 1608, 2792, 3461
EarI	CTCTTC	4 cut(s)	1128, 1608, 2792, 3461
EciI	GGCGGA	1 cut(s)	49
Eco130I	CCWWGG	7 cut(s)	65, 100, 222, 314, 464, 1455, 2707
Eco24I	GRGCYC	2 cut(s)	1542, 2962
Eco31I	GGTCTC	2 cut(s)	356, 2171
Eco47I	GGWCC	3 cut(s)	104, 311, 3302
Eco57I	CTGAAG	2 cut(s)	1271, 3055
Eco72I	CACGTG	1 cut(s)	1679
Eco88I	CYCGRG	3 cut(s)	115, 2075, 3516
Eco91I	GGTNACC	1 cut(s)	628
EcoNI	CCTNNNNNAGG	2 cut(s)	877, 1746
EcoO109I	RGGNCCY	1 cut(s)	104
EcoO65I	GGTNACC	1 cut(s)	628
EcoRI	GAATTC	2 cut(s)	856, 1793
EcoRII	CCWGG	4 cut(s)	106, 1188, 1376, 1747
EcoT14I	CCWWGG	7 cut(s)	65, 100, 222, 314, 464, 1455, 2707
EcoT22I	ATGCAT	1 cut(s)	2144
EcoT38I	GRGCYC	2 cut(s)	1542, 2962
ErhI	CCWWGG	7 cut(s)	65, 100, 222, 314, 464, 1455, 2707
FalI	AAGNNNNNCTT	6 cut(s)	215, 247, 605, 637, 1287, 1319
FaqI	GGGAC	3 cut(s)	853, 2304, 3571
FauNDI	CATATG	3 cut(s)	1633, 2483, 3134
FbaI	TGATCA	5 cut(s)	730, 951, 1339, 2109, 2355
FblI	GTMKAC	1 cut(s)	1194
Fnu4HI	GCNGC	9 cut(s)	276, 1056, 1059, 1308, 1311, 1674, 2382, 3102, 3554
FriOI	GRGCYC	2 cut(s)	1542, 2962
Fsp4HI	GCNGC	9 cut(s)	276, 1056, 1059, 1308, 1311, 1674, 2382, 3102, 3554
FspBI	CTAG	4 cut(s)	1290, 1926, 2655, 3287
GlaI	GCGC	1 cut(s)	1066
GluI	GCNGC	9 cut(s)	276, 1056, 1059, 1308, 1311, 1674, 2382, 3102, 3554
GsaI	CCCAGC	1 cut(s)	1962
GsuI	CTGGAG	1 cut(s)	27
HaeII	RGCGCY	1 cut(s)	1068
HaeIII	GGCC	5 cut(s)	463, 1188, 1623, 3084, 3140
HapII	CCGG	6 cut(s)	279, 739, 762, 1159, 1570, 3436
HgaI	GACGC	1 cut(s)	2438
HhaI	GCGC	1 cut(s)	1067
Hin6I	GCGC	1 cut(s)	1065
HinP1I	GCGC	1 cut(s)	1065
HincII	GTYRAC	3 cut(s)	1394, 2184, 3145
HindII	GTYRAC	3 cut(s)	1394, 2184, 3145
HindIII	AAGCTT	1 cut(s)	1991
HinfI	GANTC	9 cut(s)	77, 782, 1643, 2000, 2329, 2593, 2619, 3184, 3283
HpaII	CCGG	6 cut(s)	279, 739, 762, 1159, 1570, 3436
HphI	GGTGA	8 cut(s)	202, 505, 627, 832, 1638, 1789, 2080, 2579
Hpy166II	GTNNAC	6 cut(s)	1195, 1394, 1504, 2184, 3145, 3370
Hpy8I	GTNNAC	6 cut(s)	1195, 1394, 1504, 2184, 3145, 3370
HpyAV	CCTTC	9 cut(s)	223, 400, 601, 1078, 1649, 2503, 2627, 3079, 3388
HpyCH4III	ACNGT	7 cut(s)	124, 173, 679, 1280, 1444, 1896, 2934
HpyCH4IV	ACGT	4 cut(s)	1678, 1970, 2852, 3260
HpyF3I	CTNAG	8 cut(s)	353, 586, 776, 791, 1602, 2502, 2736, 3511
HpySE526I	ACGT	4 cut(s)	1678, 1970, 2852, 3260
HspAI	GCGC	1 cut(s)	1065
Kpn2I	TCCGGA	1 cut(s)	1569
KpnI	GGTACC	1 cut(s)	3226
Ksp22I	TGATCA	5 cut(s)	730, 951, 1339, 2109, 2355
LguI	GCTCTTC	1 cut(s)	1128
LmnI	GCTCC	7 cut(s)	36, 150, 318, 334, 1658, 2241, 2957
Lsp1109I	GCAGC	8 cut(s)	262, 1070, 1319, 1322, 1685, 2393, 3088, 3540
LweI	GCATC	9 cut(s)	608, 1012, 1887, 2061, 2452, 2483, 3138, 3485, 3514
MaeI	CTAG	4 cut(s)	1290, 1926, 2655, 3287
MaeII	ACGT	4 cut(s)	1678, 1970, 2852, 3260
MbiI	CCGCTC	1 cut(s)	1287
MfeI	CAATTG	2 cut(s)	2562, 3539
MflI	RGATCY	5 cut(s)	691, 1468, 2172, 2256, 2371
MhlI	GDGCHC	4 cut(s)	88, 1542, 2136, 2962
MlsI	TGGCCA	2 cut(s)	1623, 3084
MluNI	TGGCCA	2 cut(s)	1623, 3084
MlyI	GAGTC	3 cut(s)	86, 1652, 2009
MmeI	TCCRAC	5 cut(s)	463, 2263, 2343, 2566, 2596
Mox20I	TGGCCA	2 cut(s)	1623, 3084
Mph1103I	ATGCAT	1 cut(s)	2144
MroI	TCCGGA	1 cut(s)	1569
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	2 cut(s)	1623, 3084
MseI	TTAA	6 cut(s)	411, 1107, 1226, 1781, 2414, 3236
MslI	CAYNNNNRTG	5 cut(s)	1000, 1121, 1125, 1941, 3259
Msp20I	TGGCCA	2 cut(s)	1623, 3084
MspA1I	CMGCKG	2 cut(s)	1061, 2129
MspI	CCGG	6 cut(s)	279, 739, 762, 1159, 1570, 3436
MspR9I	CCNGG	6 cut(s)	108, 762, 1159, 1190, 1378, 1749
MunI	CAATTG	2 cut(s)	2562, 3539
Mva1269I	GAATGC	2 cut(s)	2347, 2801
MvaI	CCWGG	4 cut(s)	108, 1190, 1378, 1749
MvnI	CGCG	1 cut(s)	1731
NciI	CCSGG	2 cut(s)	762, 1159
NcoI	CCATGG	2 cut(s)	464, 2707
NdeI	CATATG	3 cut(s)	1633, 2483, 3134
NlaIV	GGNNCC	5 cut(s)	38, 87, 1470, 2959, 3224
NmuCI	GTSAC	5 cut(s)	1644, 1832, 2657, 3170, 3250
NsiI	ATGCAT	1 cut(s)	2144
NspI	RCATGY	5 cut(s)	1126, 2229, 2381, 2848, 3463
OliI	CACNNNNGTG	1 cut(s)	3259
PaeR7I	CTCGAG	2 cut(s)	115, 2075
PagI	TCATGA	2 cut(s)	733, 835
PciI	ACATGT	1 cut(s)	1122
PciSI	GCTCTTC	1 cut(s)	1128
PctI	GAATGC	2 cut(s)	2347, 2801
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	6 cut(s)	782, 2329, 2593, 2619, 3184, 3283
Pfl23II	CGTACG	1 cut(s)	1967
PflFI	GACNNNGTC	1 cut(s)	865
PflMI	CCANNNNNTGG	1 cut(s)	3318
PfoI	TCCNGGA	1 cut(s)	1747
PkrI	GCNGC	9 cut(s)	277, 1057, 1060, 1309, 1312, 1675, 2383, 3103, 3555
Ple19I	CGATCG	1 cut(s)	1891
PleI	GAGTC	3 cut(s)	85, 1651, 2008
PmaCI	CACGTG	1 cut(s)	1679
PmlI	CACGTG	1 cut(s)	1679
PpsI	GAGTC	3 cut(s)	85, 1651, 2008
Ppu21I	YACGTR	1 cut(s)	1679
PpuMI	RGGWCCY	1 cut(s)	104
PscI	ACATGT	1 cut(s)	1122
PsiI	TTATAA	1 cut(s)	1986
Psp1406I	AACGTT	1 cut(s)	2852
Psp5II	RGGWCCY	1 cut(s)	104
Psp6I	CCWGG	4 cut(s)	106, 1188, 1376, 1747
PspCI	CACGTG	1 cut(s)	1679
PspEI	GGTNACC	1 cut(s)	628
PspFI	CCCAGC	1 cut(s)	1958
PspGI	CCWGG	4 cut(s)	106, 1188, 1376, 1747
PspLI	CGTACG	1 cut(s)	1967
PspN4I	GGNNCC	5 cut(s)	38, 87, 1470, 2959, 3224
PspPI	GGNCC	4 cut(s)	104, 311, 3138, 3302
PspPPI	RGGWCCY	1 cut(s)	104
PspXI	VCTCGAGB	1 cut(s)	2075
PsuI	RGATCY	5 cut(s)	691, 1468, 2172, 2256, 2371
PsyI	GACNNNGTC	1 cut(s)	865
PvuI	CGATCG	1 cut(s)	1891
PvuII	CAGCTG	2 cut(s)	1061, 2129
RsaI	GTAC	9 cut(s)	113, 126, 1007, 1930, 1969, 2843, 2984, 3112, 3224
RsaNI	GTAC	9 cut(s)	112, 125, 1006, 1929, 1968, 2842, 2983, 3111, 3223
RseI	CAYNNNNRTG	5 cut(s)	1000, 1121, 1125, 1941, 3259
SapI	GCTCTTC	1 cut(s)	1128
SaqAI	TTAA	6 cut(s)	411, 1107, 1226, 1781, 2414, 3236
SatI	GCNGC	9 cut(s)	276, 1056, 1059, 1308, 1311, 1674, 2382, 3102, 3554
Sau96I	GGNCC	4 cut(s)	104, 311, 3138, 3302
SchI	GAGTC	3 cut(s)	86, 1652, 2009
ScrFI	CCNGG	6 cut(s)	108, 762, 1159, 1190, 1378, 1749
SduI	GDGCHC	4 cut(s)	88, 1542, 2136, 2962
SfaNI	GCATC	9 cut(s)	608, 1012, 1887, 2061, 2452, 2483, 3138, 3485, 3514
SfcI	CTRYAG	1 cut(s)	2124
Sfr274I	CTCGAG	2 cut(s)	115, 2075
SinI	GGWCC	3 cut(s)	104, 311, 3302
SlaI	CTCGAG	2 cut(s)	115, 2075
SmiMI	CAYNNNNRTG	5 cut(s)	1000, 1121, 1125, 1941, 3259
SmlI	CTYRAG	5 cut(s)	115, 1152, 1994, 2075, 2168
SmoI	CTYRAG	5 cut(s)	115, 1152, 1994, 2075, 2168
SsiI	CCGC	5 cut(s)	34, 1056, 1285, 1510, 1729
SspMI	CTAG	4 cut(s)	1290, 1926, 2655, 3287
StyD4I	CCNGG	6 cut(s)	106, 760, 1157, 1188, 1376, 1747
StyI	CCWWGG	7 cut(s)	65, 100, 222, 314, 464, 1455, 2707
TaaI	ACNGT	7 cut(s)	124, 173, 679, 1280, 1444, 1896, 2934
TaiI	ACGT	4 cut(s)	1681, 1973, 2855, 3263
TaqI	TCGA	8 cut(s)	116, 188, 860, 1887, 1891, 2076, 2631, 2976
TaqII	GACCGA	2 cut(s)	299, 3290
TatI	WGTACW	2 cut(s)	124, 2841
TauI	GCSGC	1 cut(s)	1058
TfiI	GAWTC	6 cut(s)	782, 2329, 2593, 2619, 3184, 3283
Tru1I	TTAA	6 cut(s)	411, 1107, 1226, 1781, 2414, 3236
Tru9I	TTAA	6 cut(s)	411, 1107, 1226, 1781, 2414, 3236
TscAI	CASTG	3 cut(s)	1764, 2629, 3197
TseFI	GTSAC	5 cut(s)	1644, 1832, 2657, 3170, 3250
TseI	GCWGC	8 cut(s)	275, 1058, 1307, 1310, 1673, 2381, 3101, 3553
Tsp45I	GTSAC	5 cut(s)	1644, 1832, 2657, 3170, 3250
TspRI	CASTG	3 cut(s)	1764, 2629, 3197
Tth111I	GACNNNGTC	1 cut(s)	865
Van91I	CCANNNNNTGG	1 cut(s)	3318
VpaK11BI	GGWCC	3 cut(s)	104, 311, 3302
XagI	CCTNNNNNAGG	2 cut(s)	877, 1746
XapI	RAATTY	9 cut(s)	512, 601, 856, 1168, 1793, 1911, 2027, 2275, 2965
XceI	RCATGY	5 cut(s)	1126, 2229, 2381, 2848, 3463
XcmI	CCANNNNNNNNNTGG	1 cut(s)	312
XhoI	CTCGAG	2 cut(s)	115, 2075
XmiI	GTMKAC	1 cut(s)	1194
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	4 cut(s)	1290, 1926, 2655, 3287
Zsp2I	ATGCAT	1 cut(s)	2144

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.