Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Reverse (-)

20262468 .. 20267859

Gene Structure 5392 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G54280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3723 bp

ATGGCTCGACGTAGAATAAGATCAAGGATTCGTAAAAGTCATTTCTATACATTTAGATGTCTTAGACCTAAAACTCTTGATGATCAAGGACCTCATGTTATCAATGGACCTGGTTACACCAGAATCGTTCATTGTAACCAGCCTCATTTGCATTTGGCTACTAAACTCATTAGGTACCGTTCAAACTATGTATCGACCACCAGGTATAACTTGCTCACTTTCTTACCTAAATGCTTGTACGAGCAATTCCACCGCGTTGCCAATTTCTATTTCCTCGTTGCTGCCATTCTCTCTGTTTTTCCTCTGTCTCCGTTTAACAAATGGAGCATGATTGCTCCTTTGGTATTCGTTGTTGGGCTTAGTATGGGGAAAGAAGCCTTGGAGGATTGGAGAAGGTTTATGCAGGACGTTGAGGTGAACTCTAGGAAAGCTAGTGTTCATAAAGGGAGTGGTGATTTTGGTAGAAGAACGTGGAAGAGAATCCGTGTTGGTGATATTGTAAGAGTGGAGAAAGATGAGTTTTTTCCTGCTGATTTGCTCTTGTTGTCATCGAGTTACGAGGATGGGATATGTTATGTTGAGACTATGAACTTAGATGGTGAAACTAACTTGAAAGTTAAGAGATGTTTGGATGCTACTTTGGCATTGGAGAAGGATGAATCTTTTCAGAACTTCTCTGGAACGATAAAATGCGAAGATCCGAATCCGAATCTGTATACGTTTGTTGGTAATCTTGAGTGTGATGGTCAGGTTTATCCACTTGATCCTAACCAGATTCTTTTGAGAGACTCAAAGCTTAGGAACACTGCTTATGTCTATGGTGTTGTGGTTTTTACTGGCCATGATACAAAAGTAATGCAGAATTCTACAAAATCTCCTTCAAAGAGAAGCAGGATTGAAAAGAGAATGGACTACATTATATATACTCTCTTTGCACTTCTCTTGACTGTTTCCTTCATCAGCTCTCTGGGTTTTGCTGTGATGACAAAACTTTTAATGGCAGAGTGGTGGTACTTAAGACCAGACAAGCCTGAGAGCTTGACAAATCCTACAAATCCTTTGTATGCGTGGGTTGTTCATTTGATCACTGCTCTGTTACTTTATGGATACTTGATTCCCATCTCATTGTATGTTTCCATTGAGGTAGTGAAAGTCTTGCAAGCACATTTTATAAATCAAGACTTGCAGTTGTATGATAGTGAGAGTGGGACTCCGGCTCAAGCACGCACCTCGAATTTAAATGAAGAGCTGGGACAAGTTGATACTATCCTTTCTGATAAAACAGGAACTTTGACATGTAACCAGATGGACTTTCTGAAATGCTCTATTGCGGGGACTTCTTACGGTGTCCGTGCTAGTGAAGTGGAACTAGCTGCTGCTAAGCAGATGGCAATGGATCTTGAGGAGAAAGGTGAAGAAGTTGCAAACCTTTCTATGAATAAAGGCAGGACACAACGGTATGCAAAATTGGCTAGCAAGACGTCATCAGATTTTGAATTGGAGACAGTAGTCACTGCTAGCGACGAAAAGGATCAGAAGCAGAACACTGGTGTTAAGGGGTTTAGTTTTGAAGATAACCGTTTGATGAATGAAAACTGGTTAAATGAGCCAAATTCAGATGACATTTTGATGTTTTTCCGTATTCTGGCGGTTTGTCATACAGCTATTCCTGAGGTGGATGAAGATACTGGAATGTGTACTTATGAAGCTGAGTCTCCTGATGAAGTTGCTTTCCTTGTTGCTTCTAGGGAGTTTGGTTTTGAATTTACTAAGAGAACTCAATCTAGTGTTTTTATTGCTGAACGGTTTTCATCTTCAGGTCAACCAGTTGATAGAGAATACAAAATCTTGAATCTGTTAGACTTCACTAGCAAAAGAAAAAGAATGTCTGCCATTGTCAGAGATGAAGAAGGGCAGATTCTTCTACTATGTAAAGGCGCTGACAGCATTATATTTGAACGTTTATCAAAGAGTGGGAAAGAGTATCTTGGAGCTACTTCAAAGCACTTGAATGTATATGGTGAAGCGGGTTTACGCACATTGGCACTTGGTTACAGAAAGCTAGATGAGACCGAGTATGCAGCATGGAACAGTGAATTTCACAAAGCCAAAACTTCAGTTGGAGCTGATAGAGATGAAATGCTCGAGAAGGTTTCAGATATGATGGAGAAGGAATTGATCCTTGTTGGAGCTACTGCTGTGGAGGACAAACTGCAAAAAGGGGTGCCTCAATGCATAGATAACCTTGCCCAAGCTGGTCTCAAAATATGGGTTTTGACAGGAGATAAGATGGAGACAGCAATAAACATAGGATATGCCTGCAGTTTACTACGACAGGGTATGAAGCAGATATCCATTTCACTTACAAATGTGGAGGAATCATCACAAAACTCTGAAGCTGCTGCAAAGGAGAGCATTTTGATGCAGATCACAAATGCTTCACAGATGATTAAAATTGAAAAGGATCCACATGCAGCATTTGCTTTGATCATTGATGGAAAAACACTTACCTATGCTTTGAAGGATGATGTCAAGTATCAGTTTCTTGCCCTTGCAGTTGACTGTGCATCAGTGATATGCTGTCGTGTCTCTCCCAAACAGAAAGCACTTGTAACAAGGCTGGCCAAAGAAGGAACAGGAAAAACAACTTTGGCAATTGGTGATGGTGCAAATGATGTTGGAATGATTCAAGAAGCTGATATTGGTGTTGGCATCAGTGGCGTTGAAGGCATGCAGGCAGTAATGGCAAGTGACTTCTCCATAGCCCAGTTTCGGTTTCTTGAGAGACTACTTGTTGTCCACGGACATTGGTGCTACAAACGAATAGCCCAGATGATTTGTTATTTCTTCTACAAGAACATAACCTTTGGTCTGACTCTCTTCTACTTCGAATGCTTTACCGGATTTTCTGGTCAATCAATATACAACGACTCCTACTTACTACTCTTCAACGTTGTTCTCACTTCTCTCCCGGTCATTTCCCTCGGAGTTTTTGAACAAGATGTTCCTTCTGATGTCTGCTTACAGTTCCCTGCATTGTATCAACAAGGCCCCAAGAACCTGTTCTTCGACTGGTACAGAATCCTTGGATGGATGGGGAATGGAGTTTATGCATCCATAGTCATCTTTACACTCAACCTTGGAATCTTCCATGTTCAATCATTCCGCTCAGATGGCCAAACCGCAGATATGAATGCCATGGGAACCGCAATGTTCACATGCATCATATGGGCAGTAAATGTTCAAATAGCTCTAACCATGAGTCACTTCACTTGGATCCAACACGTAATGATTTGGGGAAGCATAGGAGCTTGGTACGTCTTCCTCGCTCTTTACGGTATGTTACCAGTAAAACTCTCTGGTAACATCTTTCACATGCTCGTCGAGATTCTTGCCCCTGCACCCATCTTCTGGCTTACTTCACTTCTGGTCATTGCCGCCACAACGCTACCTTACTTGTTCCACATTTCATACCAAAGATCAGTGAACCCTCTTGACCATCACATTATACAAGAGATCAAACATTTCAGGATCGATGTAGAGGATGAGCGAATGTGGAAACGAGAGAAGTCAAAGGCGAGAGAGAAGACAAAGATTGGGTTCACGGCTCGAGTTGATGCTAAGATCAGGCAGCTAAGAGGCAGGCTTCAAAGGAAGCATTCGGTGTTAAGTGTTATGAGTGGAACATCGTCTAATGATACACCTTCTTCAAACTCACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005488 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006950 GO:0006996 GO:0008150 GO:0008270 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010286 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016049 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019216 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0033036 GO:0034204 GO:0040007 GO:0042623 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045332 GO:0046872 GO:0046914 GO:0048193 GO:0048194 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0060560 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0080090 GO:0097035 GO:0098791 GO:1905038

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1240

Amino Acids

140.1

Weight (kDa)

6.94

Isoelectric Point (pI)

35.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	43 - 111	1.7e-23	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	545 - 740	1.7e-17	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	914 - 1164	3.5e-87	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1172
AatII	GACGTC	1 cut(s)	1484
Acc65I	GGTACC	1 cut(s)	174
AccB1I	GGYRCC	2 cut(s)	174, 2224
AccB7I	CCANNNNNTGG	1 cut(s)	3411
AccBSI	CCGCTC	1 cut(s)	3168
AccI	GTMKAC	1 cut(s)	716
AccII	CGCG	1 cut(s)	255
AciI	CCGC	8 cut(s)	253, 1331, 1649, 2027, 3166, 3183, 3207, 3438
AclI	AACGTT	2 cut(s)	1960, 2952
AcoI	YGGCCR	3 cut(s)	838, 2622, 3175
AcsI	RAATTY	5 cut(s)	862, 1234, 1612, 1763, 2096
AcuI	CTGAAG	3 cut(s)	1800, 2100, 2415
AcyI	GRCGYC	1 cut(s)	1481
AfaI	GTAC	6 cut(s)	176, 239, 1013, 1699, 3077, 3317
AfiI	CCNNNNNNNGG	3 cut(s)	1645, 2772, 3411
AflII	CTTAAG	1 cut(s)	1015
AflIII	ACRYGT	2 cut(s)	1295, 3283
AjnI	CCWGG	2 cut(s)	109, 200
AjuI	GAANNNNNNNTTGG	8 cut(s)	254, 286, 362, 394, 2685, 2717, 3047, 3079
Ama87I	CYCGRG	2 cut(s)	2144, 3609
AoxI	GGCC	4 cut(s)	838, 2622, 3049, 3175
ApeKI	GCWGC	8 cut(s)	281, 1373, 1376, 2081, 2399, 2402, 2474, 3631
ApoI	RAATTY	5 cut(s)	862, 1234, 1612, 1763, 2096
Asp700I	GAANNNNTTC	2 cut(s)	663, 3062
Asp718I	GGTACC	1 cut(s)	174
AspLEI	GCGC	1 cut(s)	1940
AspS9I	GGNCC	3 cut(s)	89, 107, 3050
AsuC2I	CCSGG	1 cut(s)	2972
AsuHPI	GGTGA	7 cut(s)	427, 464, 503, 611, 1424, 2033, 2672
AsuII	TTCGAA	1 cut(s)	2889
AsuNHI	GCTAGC	2 cut(s)	1472, 1517
AvaI	CYCGRG	2 cut(s)	2144, 3609
AvaII	GGWCC	2 cut(s)	89, 107
AxyI	CCTNAGG	1 cut(s)	1671
BaeI	ACNNNNGTAYC	2 cut(s)	837, 870
BalI	TGGCCA	3 cut(s)	840, 2624, 3177
BamHI	GGATCC	2 cut(s)	2464, 3276
BanI	GGYRCC	2 cut(s)	174, 2224
BarI	GAAGNNNNNNTAC	2 cut(s)	2016, 2048
BbsI	GAAGAC	2 cut(s)	3313, 3593
BbvI	GCAGC	8 cut(s)	268, 1360, 1363, 2093, 2386, 2389, 2486, 3643
BceAI	ACGGC	1 cut(s)	3621
BcgI	CGANNNNNNTGC	4 cut(s)	1212, 1246, 3374, 3408
BciT130I	CCWGG	2 cut(s)	111, 202
BciVI	GTATCC	1 cut(s)	1100
BclI	TGATCA	3 cut(s)	82, 1083, 2487
BcnI	CCSGG	1 cut(s)	2972
BfmI	CTRYAG	1 cut(s)	2320
BfoI	RGCGCY	1 cut(s)	1941
BfrI	CTTAAG	1 cut(s)	1015
BfuI	GTATCC	1 cut(s)	1100
BisI	GCNGC	9 cut(s)	282, 1374, 1377, 2082, 2400, 2403, 2475, 3438, 3632
BlpI	GCTNAGC	1 cut(s)	1380
BlsI	GCNGC	9 cut(s)	283, 1375, 1378, 2083, 2401, 2404, 2476, 3439, 3633
Bme1390I	CCNGG	3 cut(s)	111, 202, 2972
Bme18I	GGWCC	2 cut(s)	89, 107
BmeT110I	CYCGRG	2 cut(s)	2144, 3609
BmgT120I	GGNCC	3 cut(s)	89, 107, 3050
BmiI	GGNNCC	6 cut(s)	176, 2226, 2466, 3052, 3205, 3278
BmrFI	CCNGG	3 cut(s)	111, 202, 2972
BmrI	ACTGGG	1 cut(s)	2761
BmsI	GCATC	7 cut(s)	622, 2412, 2576, 2721, 3122, 3231, 3607
BmtI	GCTAGC	2 cut(s)	1476, 1521
BmuI	ACTGGG	1 cut(s)	2761
BoxI	GACNNNNGTC	1 cut(s)	1508
BpiI	GAAGAC	2 cut(s)	3313, 3593
BplI	GAGNNNNNCTC	4 cut(s)	404, 436, 1195, 1227
Bpu10I	CCTNAGC	1 cut(s)	797
Bpu1102I	GCTNAGC	1 cut(s)	1380
Bpu14I	TTCGAA	1 cut(s)	2889
BpuEI	CTTGAG	4 cut(s)	755, 1203, 1421, 2801
BpuMI	CCSGG	1 cut(s)	2972
Bsa29I	ATCGAT	1 cut(s)	3534
BsaAI	YACGTR	1 cut(s)	3286
BsaBI	GATNNNNATC	3 cut(s)	702, 1118, 2426
BsaHI	GRCGYC	1 cut(s)	1481
BsaI	GGTCTC	2 cut(s)	2063, 2264
BsaJI	CCNNGG	6 cut(s)	378, 2800, 2983, 3085, 3139, 3198
BsaWI	WCCGGW	1 cut(s)	2900
BsaXI	ACNNNNNCTCC	4 cut(s)	859, 889, 2915, 2945
Bsc4I	CCNNNNNNNGG	3 cut(s)	1645, 2772, 3411
Bse1I	ACTGG	8 cut(s)	841, 1552, 1601, 1693, 1826, 2767, 3077, 3347
Bse21I	CCTNAGG	1 cut(s)	1671
Bse3DI	GCAATG	3 cut(s)	1398, 3216, 3432
Bse8I	GATNNNNATC	3 cut(s)	702, 1118, 2426
BseBI	CCWGG	2 cut(s)	111, 202
BseCI	ATCGAT	1 cut(s)	3534
BseDI	CCNNGG	6 cut(s)	378, 2800, 2983, 3085, 3139, 3198
BseGI	GGATG	9 cut(s)	568, 637, 661, 1684, 2530, 3095, 3099, 3113, 3550
BseJI	GATNNNNATC	3 cut(s)	702, 1118, 2426
BseLI	CCNNNNNNNGG	3 cut(s)	1645, 2772, 3411
BseMI	GCAATG	3 cut(s)	1398, 3216, 3432
BseMII	CTCAG	4 cut(s)	1023, 1662, 1701, 3183
BseNI	ACTGG	8 cut(s)	841, 1552, 1601, 1693, 1826, 2767, 3077, 3347
BseRI	GAGGAG	1 cut(s)	1418
BseXI	GCAGC	8 cut(s)	268, 1360, 1363, 2093, 2386, 2389, 2486, 3643
BseYI	CCCAGC	1 cut(s)	1249
BsgI	GTGCAG	1 cut(s)	3384
Bsh1236I	CGCG	1 cut(s)	255
BshFI	GGCC	4 cut(s)	840, 2624, 3051, 3177
BshNI	GGYRCC	2 cut(s)	174, 2224
BshVI	ATCGAT	1 cut(s)	3534
BsiHKCI	CYCGRG	2 cut(s)	2144, 3609
BsiSI	CCGG	3 cut(s)	1214, 2901, 2972
BslFI	GGGAC	3 cut(s)	1222, 1266, 1348
BslI	CCNNNNNNNGG	3 cut(s)	1645, 2772, 3411
BsmFI	GGGAC	3 cut(s)	1222, 1266, 1348
BsmI	GAATGC	3 cut(s)	2897, 3199, 3658
BsnI	GGCC	4 cut(s)	840, 2624, 3051, 3177
Bso31I	GGTCTC	2 cut(s)	2063, 2264
BsoBI	CYCGRG	2 cut(s)	2144, 3609
Bsp119I	TTCGAA	1 cut(s)	2889
Bsp1720I	GCTNAGC	1 cut(s)	1380
Bsp19I	CCATGG	1 cut(s)	3198
BspACI	CCGC	8 cut(s)	253, 1331, 1649, 2027, 3166, 3183, 3207, 3438
BspANI	GGCC	4 cut(s)	840, 2624, 3051, 3177
BspCNI	CTCAG	4 cut(s)	1024, 1663, 1702, 3182
BspDI	ATCGAT	1 cut(s)	3534
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	6 cut(s)	176, 2226, 2466, 3052, 3205, 3278
BspMAI	CTGCAG	1 cut(s)	2324
BspOI	GCTAGC	2 cut(s)	1476, 1521
BspQI	GCTCTTC	1 cut(s)	1239
BspT104I	TTCGAA	1 cut(s)	2889
BspT107I	GGYRCC	2 cut(s)	174, 2224
BspTI	CTTAAG	1 cut(s)	1015
BspTNI	GGTCTC	2 cut(s)	2063, 2264
BsrBI	CCGCTC	1 cut(s)	3168
BsrDI	GCAATG	3 cut(s)	1398, 3216, 3432
BsrI	ACTGG	8 cut(s)	841, 1552, 1601, 1693, 1826, 2767, 3077, 3347
BssECI	CCNNGG	6 cut(s)	378, 2800, 2983, 3085, 3139, 3198
BssNAI	GTATAC	1 cut(s)	717
BssNI	GRCGYC	1 cut(s)	1481
BssT1I	CCWWGG	4 cut(s)	378, 3085, 3139, 3198
Bst1107I	GTATAC	1 cut(s)	717
Bst2UI	CCWGG	2 cut(s)	111, 202
Bst6I	CTCTTC	4 cut(s)	470, 1239, 2885, 2951
BstACI	GRCGYC	1 cut(s)	1481
BstAFI	CTTAAG	1 cut(s)	1015
BstAPI	GCANNNNNTGC	1 cut(s)	2480
BstBAI	YACGTR	1 cut(s)	3286
BstBI	TTCGAA	1 cut(s)	2889
BstC8I	GCNNGC	9 cut(s)	1161, 1225, 1474, 1519, 2320, 2622, 2732, 2736, 3644
BstDSI	CCRYGG	2 cut(s)	2800, 3198
BstF5I	GGATG	9 cut(s)	568, 637, 661, 1684, 2530, 3095, 3099, 3113, 3550
BstFNI	CGCG	1 cut(s)	255
BstH2I	RGCGCY	1 cut(s)	1941
BstHHI	GCGC	1 cut(s)	1940
BstMWI	GCNNNNNNNGC	9 cut(s)	148, 641, 1469, 1944, 2480, 2718, 2727, 2744, 3174
BstNI	CCWGG	2 cut(s)	111, 202
BstNSI	RCATGY	5 cut(s)	1299, 2474, 2734, 3222, 3379
BstPAI	GACNNNNGTC	1 cut(s)	1508
BstSCI	CCNGG	3 cut(s)	109, 200, 2970
BstSFI	CTRYAG	1 cut(s)	2320
BstUI	CGCG	1 cut(s)	255
BstV1I	GCAGC	8 cut(s)	268, 1360, 1363, 2093, 2386, 2389, 2486, 3643
BstV2I	GAAGAC	2 cut(s)	3313, 3593
BstX2I	RGATCY	4 cut(s)	697, 1396, 2464, 3276
BstYI	RGATCY	4 cut(s)	697, 1396, 2464, 3276
BstZ17I	GTATAC	1 cut(s)	717
Bsu15I	ATCGAT	1 cut(s)	3534
Bsu36I	CCTNAGG	1 cut(s)	1671
BsuI	GTATCC	1 cut(s)	1100
BsuRI	GGCC	4 cut(s)	840, 2624, 3051, 3177
BsuTUI	ATCGAT	1 cut(s)	3534
BtgI	CCRYGG	2 cut(s)	2800, 3198
BtsCI	GGATG	9 cut(s)	568, 637, 661, 1684, 2530, 3095, 3099, 3113, 3550
BtsI	GCAGTG	3 cut(s)	804, 1086, 1512
BtsIMutI	CAGTG	8 cut(s)	804, 1086, 1512, 1545, 2098, 2577, 2722, 3489
Cac8I	GCNNGC	9 cut(s)	1161, 1225, 1474, 1519, 2320, 2622, 2732, 2736, 3644
CfoI	GCGC	1 cut(s)	1940
Cfr13I	GGNCC	3 cut(s)	89, 107, 3050
ClaI	ATCGAT	1 cut(s)	3534
CsiI	ACCWGGT	2 cut(s)	109, 200
Csp6I	GTAC	6 cut(s)	175, 238, 1012, 1698, 3076, 3316
CspCI	CAANNNNNGTGG	2 cut(s)	2789, 2824
CviQI	GTAC	6 cut(s)	175, 238, 1012, 1698, 3076, 3316
DraI	TTTAAA	1 cut(s)	1239
EaeI	YGGCCR	3 cut(s)	838, 2622, 3175
Eam1104I	CTCTTC	4 cut(s)	470, 1239, 2885, 2951
EarI	CTCTTC	4 cut(s)	470, 1239, 2885, 2951
Eco130I	CCWWGG	4 cut(s)	378, 3085, 3139, 3198
Eco31I	GGTCTC	2 cut(s)	2063, 2264
Eco32I	GATATC	1 cut(s)	2352
Eco47I	GGWCC	2 cut(s)	89, 107
Eco57I	CTGAAG	3 cut(s)	1800, 2100, 2415
Eco81I	CCTNAGG	1 cut(s)	1671
Eco88I	CYCGRG	2 cut(s)	2144, 3609
EcoO109I	RGGNCCY	2 cut(s)	89, 3050
EcoRI	GAATTC	1 cut(s)	862
EcoRII	CCWGG	2 cut(s)	109, 200
EcoRV	GATATC	1 cut(s)	2352
EcoT14I	CCWWGG	4 cut(s)	378, 3085, 3139, 3198
EcoT22I	ATGCAT	3 cut(s)	2237, 3115, 3224
ErhI	CCWWGG	4 cut(s)	378, 3085, 3139, 3198
FalI	AAGNNNNNCTT	2 cut(s)	1971, 2003
FaqI	GGGAC	3 cut(s)	1222, 1266, 1348
FauI	CCCGC	2 cut(s)	1324, 2020
FauNDI	CATATG	1 cut(s)	3227
FbaI	TGATCA	3 cut(s)	82, 1083, 2487
FblI	GTMKAC	1 cut(s)	716
Fnu4HI	GCNGC	9 cut(s)	282, 1374, 1377, 2082, 2400, 2403, 2475, 3438, 3632
FokI	GGATG	9 cut(s)	575, 644, 668, 1691, 2537, 3100, 3102, 3106, 3557
Fsp4HI	GCNGC	9 cut(s)	282, 1374, 1377, 2082, 2400, 2403, 2475, 3438, 3632
GlaI	GCGC	1 cut(s)	1939
GluI	GCNGC	9 cut(s)	282, 1374, 1377, 2082, 2400, 2403, 2475, 3438, 3632
GsaI	CCCAGC	1 cut(s)	1253
HaeII	RGCGCY	1 cut(s)	1941
HaeIII	GGCC	4 cut(s)	840, 2624, 3051, 3177
HapII	CCGG	3 cut(s)	1214, 2901, 2972
HhaI	GCGC	1 cut(s)	1940
Hin1I	GRCGYC	1 cut(s)	1481
Hin6I	GCGC	1 cut(s)	1938
HinP1I	GCGC	1 cut(s)	1938
HincII	GTYRAC	2 cut(s)	1823, 2560
HindII	GTYRAC	2 cut(s)	1823, 2560
HindIII	AAGCTT	1 cut(s)	794
HpaII	CCGG	3 cut(s)	1214, 2901, 2972
HphI	GGTGA	7 cut(s)	427, 464, 503, 611, 1424, 2033, 2672
Hpy99I	CGWCG	3 cut(s)	12, 1526, 3386
HpyCH4IV	ACGT	9 cut(s)	10, 408, 470, 719, 1481, 1960, 2952, 3285, 3318
HpyF10VI	GCNNNNNNNGC	9 cut(s)	148, 641, 1469, 1944, 2480, 2718, 2727, 2744, 3174
HpySE526I	ACGT	9 cut(s)	10, 408, 470, 719, 1481, 1960, 2952, 3285, 3318
Hsp92I	GRCGYC	1 cut(s)	1481
HspAI	GCGC	1 cut(s)	1938
KpnI	GGTACC	1 cut(s)	178
Ksp22I	TGATCA	3 cut(s)	82, 1083, 2487
LguI	GCTCTTC	1 cut(s)	1239
LmnI	GCTCC	6 cut(s)	324, 340, 1991, 2123, 2189, 3308
Lsp1109I	GCAGC	8 cut(s)	268, 1360, 1363, 2093, 2386, 2389, 2486, 3643
LweI	GCATC	7 cut(s)	622, 2412, 2576, 2721, 3122, 3231, 3607
MabI	ACCWGGT	2 cut(s)	109, 200
MaeII	ACGT	9 cut(s)	10, 408, 470, 719, 1481, 1960, 2952, 3285, 3318
MbiI	CCGCTC	1 cut(s)	3168
MfeI	CAATTG	1 cut(s)	2655
MflI	RGATCY	4 cut(s)	697, 1396, 2464, 3276
MlsI	TGGCCA	3 cut(s)	840, 2624, 3177
MluNI	TGGCCA	3 cut(s)	840, 2624, 3177
MlyI	GAGTC	6 cut(s)	782, 1204, 1721, 2869, 2924, 3271
MmeI	TCCRAC	4 cut(s)	2101, 2167, 2659, 3304
Mox20I	TGGCCA	3 cut(s)	840, 2624, 3177
Mph1103I	ATGCAT	3 cut(s)	2237, 3115, 3224
MroXI	GAANNNNTTC	2 cut(s)	663, 3062
MscI	TGGCCA	3 cut(s)	840, 2624, 3177
MseI	TTAA	9 cut(s)	315, 618, 995, 1016, 1238, 1554, 1601, 2451, 3668
MslI	CAYNNNNRTG	4 cut(s)	55, 1628, 2010, 2420
Msp20I	TGGCCA	3 cut(s)	840, 2624, 3177
MspCI	CTTAAG	1 cut(s)	1015
MspI	CCGG	3 cut(s)	1214, 2901, 2972
MspR9I	CCNGG	3 cut(s)	111, 202, 2972
MunI	CAATTG	1 cut(s)	2655
Mva1269I	GAATGC	3 cut(s)	2897, 3199, 3658
MvaI	CCWGG	2 cut(s)	111, 202
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	9 cut(s)	148, 641, 1469, 1944, 2480, 2718, 2727, 2744, 3174
NciI	CCSGG	1 cut(s)	2972
NcoI	CCATGG	1 cut(s)	3198
NdeI	CATATG	1 cut(s)	3227
NheI	GCTAGC	2 cut(s)	1472, 1517
NlaIV	GGNNCC	6 cut(s)	176, 2226, 2466, 3052, 3205, 3278
NmuCI	GTSAC	3 cut(s)	1510, 2750, 3263
NsiI	ATGCAT	3 cut(s)	2237, 3115, 3224
NspI	RCATGY	5 cut(s)	1299, 2474, 2734, 3222, 3379
NspV	TTCGAA	1 cut(s)	2889
PaeI	GCATGC	1 cut(s)	2734
PaeR7I	CTCGAG	2 cut(s)	2144, 3609
PciI	ACATGT	1 cut(s)	1295
PciSI	GCTCTTC	1 cut(s)	1239
PctI	GAATGC	3 cut(s)	2897, 3199, 3658
PdmI	GAANNNNTTC	2 cut(s)	663, 3062
PflMI	CCANNNNNTGG	1 cut(s)	3411
PkrI	GCNGC	9 cut(s)	283, 1375, 1378, 2083, 2401, 2404, 2476, 3439, 3633
PleI	GAGTC	6 cut(s)	782, 1204, 1720, 2869, 2924, 3270
PpsI	GAGTC	6 cut(s)	782, 1204, 1720, 2869, 2924, 3270
Ppu21I	YACGTR	1 cut(s)	3286
PpuMI	RGGWCCY	1 cut(s)	89
PscI	ACATGT	1 cut(s)	1295
PshAI	GACNNNNGTC	1 cut(s)	1508
PsiI	TTATAA	1 cut(s)	1172
Psp1406I	AACGTT	2 cut(s)	1960, 2952
Psp5II	RGGWCCY	1 cut(s)	89
Psp6I	CCWGG	2 cut(s)	109, 200
PspFI	CCCAGC	1 cut(s)	1249
PspGI	CCWGG	2 cut(s)	109, 200
PspN4I	GGNNCC	6 cut(s)	176, 2226, 2466, 3052, 3205, 3278
PspPI	GGNCC	3 cut(s)	89, 107, 3050
PspPPI	RGGWCCY	1 cut(s)	89
PspXI	VCTCGAGB	1 cut(s)	3609
PstI	CTGCAG	1 cut(s)	2324
PsuI	RGATCY	4 cut(s)	697, 1396, 2464, 3276
RsaI	GTAC	6 cut(s)	176, 239, 1013, 1699, 3077, 3317
RsaNI	GTAC	6 cut(s)	175, 238, 1012, 1698, 3076, 3316
RseI	CAYNNNNRTG	4 cut(s)	55, 1628, 2010, 2420
SapI	GCTCTTC	1 cut(s)	1239
SaqAI	TTAA	9 cut(s)	315, 618, 995, 1016, 1238, 1554, 1601, 2451, 3668
SatI	GCNGC	9 cut(s)	282, 1374, 1377, 2082, 2400, 2403, 2475, 3438, 3632
Sau96I	GGNCC	3 cut(s)	89, 107, 3050
SchI	GAGTC	6 cut(s)	782, 1204, 1721, 2869, 2924, 3271
ScrFI	CCNGG	3 cut(s)	111, 202, 2972
SexAI	ACCWGGT	2 cut(s)	109, 200
SfaNI	GCATC	7 cut(s)	622, 2412, 2576, 2721, 3122, 3231, 3607
SfcI	CTRYAG	1 cut(s)	2320
Sfr274I	CTCGAG	2 cut(s)	2144, 3609
SfuI	TTCGAA	1 cut(s)	2889
SinI	GGWCC	2 cut(s)	89, 107
SlaI	CTCGAG	2 cut(s)	2144, 3609
SmiI	ATTTAAAT	1 cut(s)	1239
SmiMI	CAYNNNNRTG	4 cut(s)	55, 1628, 2010, 2420
SmlI	CTYRAG	7 cut(s)	734, 1015, 1218, 1400, 2144, 2780, 3609
SmoI	CTYRAG	7 cut(s)	734, 1015, 1218, 1400, 2144, 2780, 3609
SphI	GCATGC	1 cut(s)	2734
SsiI	CCGC	8 cut(s)	253, 1331, 1649, 2027, 3166, 3183, 3207, 3438
StyD4I	CCNGG	3 cut(s)	109, 200, 2970
StyI	CCWWGG	4 cut(s)	378, 3085, 3139, 3198
SwaI	ATTTAAAT	1 cut(s)	1239
TaiI	ACGT	9 cut(s)	13, 411, 473, 722, 1484, 1963, 2955, 3288, 3321
TaqII	GACCGA	1 cut(s)	2087
TatI	WGTACW	1 cut(s)	1697
TauI	GCSGC	1 cut(s)	3440
Tru1I	TTAA	9 cut(s)	315, 618, 995, 1016, 1238, 1554, 1601, 2451, 3668
Tru9I	TTAA	9 cut(s)	315, 618, 995, 1016, 1238, 1554, 1601, 2451, 3668
TscAI	CASTG	8 cut(s)	811, 1093, 1519, 1552, 2098, 2577, 2722, 3489
TseFI	GTSAC	3 cut(s)	1510, 2750, 3263
TseI	GCWGC	8 cut(s)	281, 1373, 1376, 2081, 2399, 2402, 2474, 3631
Tsp45I	GTSAC	3 cut(s)	1510, 2750, 3263
TspGWI	ACGGA	5 cut(s)	300, 473, 1340, 1628, 2817
TspRI	CASTG	8 cut(s)	811, 1093, 1519, 1552, 2098, 2577, 2722, 3489
Van91I	CCANNNNNTGG	1 cut(s)	3411
Vha464I	CTTAAG	1 cut(s)	1015
VpaK11BI	GGWCC	2 cut(s)	89, 107
XapI	RAATTY	5 cut(s)	862, 1234, 1612, 1763, 2096
XceI	RCATGY	5 cut(s)	1299, 2474, 2734, 3222, 3379
XhoI	CTCGAG	2 cut(s)	2144, 3609
XmiI	GTMKAC	1 cut(s)	716
XmnI	GAANNNNTTC	2 cut(s)	663, 3062
ZraI	GACGTC	1 cut(s)	1482
Zsp2I	ATGCAT	3 cut(s)	2237, 3115, 3224

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.