AT1G18485

pentatricopeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016120
Physical Location & Seq
Forward (+)
6363042 .. 6366263
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18485.1

Sequence Viewer

Length: 2913 bp
ATGGCTTCTGTTTTGCTTCCATTGCCACAAGTATTTGTTTTGTTTGATTATCGCAGGAGCCGTAAAGAGTCAAGCTTTCCCCGCGCCGTCTACAATTCCAATTCCATTTCTTCTAATTCAACCAATGCCAATCATTTCCTTCGCCGGATTTCTAACTTCTGTGAAACCGGAGACCTCGACAAGTCCTTTCGAACTGTCCAAGAATTCGTTGGCGATGACGAGAGCTCGTCCGATGCGTTTCTTCTTGTCCGAGAAGCTCTTGGGCTTCTCTTACAAGCTTCTGGGAAGAGAAAAGACATTGAAATGGGAAGAAAGATCCACCAGCTGGTTTCTGGGTCGACCCGGTTAAGGAATGATGACGTTCTCTGTACTCGTATTATCACCATGTACGCTATGTGTGGCTCTCCTGATGATTCTCGGTTTGTGTTTGATGCCTTGCGGAGTAAGAATCTCTTCCAGTGGAATGCTGTTATCAGCTCTTACTCAAGAAACGAGCTTTACGATGAAGTATTAGAGACGTTTATCGAGATGATCTCAACGACTGATCTTTTACCGGATCATTTCACTTATCCATGTGTGATTAAGGCTTGTGCTGGGATGTCTGATGTTGGGATCGGGCTTGCGGTTCATGGGTTGGTGGTGAAGACCGGATTGGTGGAAGATGTTTTTGTGGGTAATGCATTGGTGTCTTTCTATGGAACTCATGGTTTTGTTACTGATGCCTTGCAACTGTTCGATATAATGCCTGAGAGAAATTTGGTTTCCTGGAACTCCATGATTCGAGTGTTCTCGGATAATGGTTTCTCAGAAGAAAGCTTCTTGCTTCTTGGAGAAATGATGGAGGAAAATGGTGATGGTGCGTTTATGCCGGATGTTGCTACACTTGTTACTGTGTTGCCGGTTTGTGCAAGAGAAAGAGAAATAGGGTTGGGAAAAGGTGTTCATGGGTGGGCGGTGAAATTGAGGTTGGACAAGGAACTAGTCCTTAACAATGCCTTAATGGACATGTACTCCAAATGCGGTTGCATTACTAATGCACAAATGATCTTTAAGATGAACAATAACAAGAATGTGGTTTCTTGGAACACAATGGTAGGAGGTTTTTCAGCAGAGGGTGATACACATGGGACGTTTGATGTTCTGAGACAGATGCTGGCAGGAGGCGAAGATGTCAAAGCGGACGAGGTAACCATTCTAAATGCAGTGCCTGTTTGTTTCCATGAATCATTCTTACCGAGTTTAAAGGAACTTCATTGTTACTCCTTAAAACAAGAGTTTGTATACAATGAGTTAGTAGCCAATGCCTTTGTGGCTTCTTATGCAAAGTGTGGCTCACTGAGTTATGCCCAGCGTGTGTTTCATGGTATAAGGAGTAAAACAGTGAACTCTTGGAATGCTTTGATTGGCGGCCATGCTCAGAGCAATGATCCGAGATTGTCCTTGGATGCTCATTTGCAGATGAAAATTTCTGGATTGCTACCTGATAGTTTTACTGTCTGTAGTCTTCTTTCAGCTTGTTCTAAACTAAAATCCCTGCGGTTGGGAAAGGAAGTGCATGGGTTTATAATCCGAAATTGGTTAGAAAGAGATTTGTTTGTTTATTTATCAGTACTCTCATTATACATTCATTGTGGGGAGCTATGCACTGTACAAGCATTATTTGATGCAATGGAAGATAAAAGCTTAGTATCTTGGAATACTGTAATCACCGGTTACCTTCAGAATGGATTTCCTGATAGAGCTTTGGGCGTCTTTAGACAAATGGTTTTGTATGGAATCCAGCTTTGTGGGATTTCTATGATGCCTGTGTTTGGTGCGTGTTCGCTGCTACCTTCTCTTCGACTGGGGAGAGAAGCGCATGCTTATGCTTTGAAGCACCTCCTGGAGGACGATGCGTTTATTGCATGTTCTTTAATTGATATGTATGCCAAGAATGGTTCTATAACACAATCATCTAAGGTTTTCAATGGCTTGAAGGAGAAAAGCACGGCATCTTGGAATGCCATGATAATGGGATATGGAATACATGGCCTAGCAAAAGAGGCTATAAAACTCTTTGAAGAGATGCAAAGAACAGGTCATAACCCTGATGACCTAACTTTCTTAGGTGTTCTAACGGCTTGCAACCATAGTGGTCTGATCCATGAAGGGCTGAGATATCTGGATCAAATGAAGTCTTCTTTTGGGCTAAAGCCAAATTTAAAGCATTATGCTTGTGTCATTGACATGTTAGGCCGAGCTGGGCAACTAGACAAGGCTCTAAGGGTTGTTGCAGAAGAGATGTCTGAGGAAGCTGACGTTGGAATTTGGAAGTCTCTGCTCAGCTCATGCAGAATCCATCAAAATCTGGAAATGGGAGAGAAAGTTGCTGCTAAGTTATTCGAATTAGAACCAGAAAAGCCTGAGAATTATGTTCTGCTTTCCAACTTATATGCAGGATTGGGGAAATGGGAAGACGTGAGAAAAGTGAGACAGAGAATGAACGAGATGAGTTTACGAAAGGATGCTGGATGCTCTTGGATCGAACTAAACAGGAAGGTATTTAGCTTTGTTGTCGGTGAAAGATTCTTAGATGGTTTTGAGGAAATCAAAAGTCTTTGGAGTATATTGGAGATGAAGATATCGAAGATGGGATACAGACCAGACACAATGTCAGTACAACATGATCTAAGTGAAGAGGAGAAGATTGAACAGTTAAGGGGACACAGCGAGAAGCTTGCCCTTACCTACGGGTTGATTAAAACTTCAGAAGGAACAACTATTAGAGTTTACAAGAACCTAAGGATCTGTGTGGACTGTCACAATGCAGCAAAGTTGATCTCCAAGGTCATGGAAAGGGAAATAGTTGTGAGGGACAACAAAAGGTTTCATCATTTCAAGAATGGGGTTTGTTCTTGTGGAGATTACTGGTAA

Protein Analysis

970

Amino Acids

108.81

Weight (kDa)

6.22

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 150 - 198 6.9e-06 PPR repeat family
PPR_2 PF13041 460 - 508 2.4e-06 PPR repeat family
PPR PF01535 563 - 593 4.9e-07 PPR repeat
PPR_2 PF13041 662 - 708 2.7e-10 PPR repeat family
PPR PF01535 665 - 693 4.4e-08 PPR repeat
E_motif PF20431 780 - 842 2.2e-20 E motif
DYW_deaminase PF14432 878 - 970 2.8e-42 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18485
fragaria_vesca FvH4_5g13240
malus_domestica MD06G1207000.v1.1
prunus_persica Prupe.5G211400_v2.0.a1
pyrus_communis pycom06g18480
rosa_chinensis RchiOBHm_Chr7g0183921
rosa_laevigata RLG00000005010
rosa_multiflora Rmu_sc0004712.1_g000029 Rmu_sc0007533.1_g000009
rosa_rugosa Rorug06G0461400
rosa_samantha Rh7AG062600 Rh7BG062800 Rh7CG064100 Rh7DG062600
rosa_wichuraiana Rw7G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1565
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 3 cut(s) 90, 338, 1281
AccII CGCG 1 cut(s) 84
AciI CCGC 8 cut(s) 82, 439, 623, 953, 1020, 1178, 1407, 1537
AclWI GGATC 8 cut(s) 310, 564, 620, 1421, 2134, 2172, 2528, 2792
AcoI YGGCCR 1 cut(s) 1408
AcsI RAATTY 5 cut(s) 203, 754, 1464, 2197, 2304
AcuI CTGAAG 2 cut(s) 1703, 2730
AcyI GRCGYC 1 cut(s) 1749
AfaI GTAC 6 cut(s) 370, 389, 1010, 1611, 1650, 2658
AfiI CCNNNNNNNGG 5 cut(s) 325, 348, 1540, 1811, 1885
AflIII ACRYGT 2 cut(s) 1005, 2226
AgeI ACCGGT 1 cut(s) 1709
AgsI TTSAA 8 cut(s) 120, 302, 1873, 1966, 1975, 2060, 2690, 2878
AhdI GACNNNNNGTC 1 cut(s) 2650
AhlI ACTAGT 1 cut(s) 979
AjiI CACGTC 1 cut(s) 2458
AjnI CCWGG 2 cut(s) 764, 1881
AjuI GAANNNNNNNTTGG 6 cut(s) 635, 667, 950, 982, 2283, 2315
AloI GAACNNNNNNTCC 2 cut(s) 924, 956
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 5 cut(s) 165, 509, 1138, 2319, 2464
AlwI GGATC 8 cut(s) 310, 564, 620, 1421, 2134, 2172, 2528, 2792
AlwNI CAGNNNCTG 2 cut(s) 1153, 1208
AoxI GGCC 3 cut(s) 1408, 2029, 2233
ApeKI GCWGC 3 cut(s) 1825, 2369, 2807
ApoI RAATTY 5 cut(s) 203, 754, 1464, 2197, 2304
ArsI GACNNNNNNTTYG 2 cut(s) 2062, 2094
AsiGI ACCGGT 1 cut(s) 1709
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 86, 1858
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 7 cut(s) 373, 652, 863, 967, 1127, 1699, 2570
AsuII TTCGAA 2 cut(s) 190, 2382
AxyI CCTNAGG 1 cut(s) 2780
BanII GRGCYC 1 cut(s) 227
BarI GAAGNNNNNNTAC 2 cut(s) 2618, 2650
BbsI GAAGAC 4 cut(s) 650, 1496, 2169, 2460
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 1812, 2356, 2819
BccI CCATC 5 cut(s) 832, 848, 2346, 2567, 2623
BceAI ACGGC 4 cut(s) 45, 71, 2004, 2133
BcgI CGANNNNNNTGC 2 cut(s) 2699, 2733
BciT130I CCWGG 2 cut(s) 766, 1883
BciVI GTATCC 1 cut(s) 2627
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 5 cut(s) 165, 509, 1138, 2319, 2464
BcuI ACTAGT 1 cut(s) 979
BfaI CTAG 3 cut(s) 980, 2033, 2249
BfmI CTRYAG 1 cut(s) 1498
BfuI GTATCC 1 cut(s) 2627
BglI GCCNNNNNGGC 1 cut(s) 1310
BisI GCNGC 4 cut(s) 1408, 1826, 2370, 2808
BlpI GCTNAGC 1 cut(s) 2321
BlsI GCNGC 4 cut(s) 1409, 1827, 2371, 2809
BmcAI AGTACT 1 cut(s) 1611
Bme1390I CCNGG 3 cut(s) 343, 766, 1883
BmeRI GACNNNNNGTC 1 cut(s) 2650
BmgBI CACGTC 1 cut(s) 2458
BmiI GGNNCC 1 cut(s) 59
BmrFI CCNGG 3 cut(s) 343, 766, 1883
BmrI ACTGGG 1 cut(s) 1853
BmuI ACTGGG 1 cut(s) 1853
BpiI GAAGAC 4 cut(s) 650, 1496, 2169, 2460
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 1 cut(s) 1904
Bpu1102I GCTNAGC 1 cut(s) 2321
Bpu14I TTCGAA 2 cut(s) 190, 2382
BpuEI CTTGAG 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 1749
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 1440, 2823
BsaWI WCCGGW 4 cut(s) 167, 553, 647, 1709
BsaXI ACNNNNNCTCC 4 cut(s) 995, 1025, 2349, 2379
Bsc4I CCNNNNNNNGG 5 cut(s) 325, 348, 1540, 1811, 1885
Bse118I RCCGGY 2 cut(s) 898, 1709
Bse1I ACTGG 3 cut(s) 457, 1848, 2912
Bse21I CCTNAGG 1 cut(s) 2780
Bse3DI GCAATG 3 cut(s) 20, 1429, 1674
BseBI CCWGG 2 cut(s) 766, 1883
BseDI CCNNGG 2 cut(s) 1440, 2823
BseGI GGATG 5 cut(s) 603, 877, 1450, 2509, 2516
BseLI CCNNNNNNNGG 5 cut(s) 325, 348, 1540, 1811, 1885
BseMI GCAATG 3 cut(s) 20, 1429, 1674
BseMII CTCAG 9 cut(s) 738, 819, 1133, 1328, 1430, 2144, 2277, 2335, 2394
BseNI ACTGG 3 cut(s) 457, 1848, 2912
BseRI GAGGAG 1 cut(s) 2693
BseXI GCAGC 3 cut(s) 1812, 2356, 2819
BseYI CCCAGC 3 cut(s) 593, 1347, 2240
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 1410, 2031, 2235
BshTI ACCGGT 1 cut(s) 1709
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 8 cut(s) 145, 168, 343, 554, 648, 869, 899, 1710
BslFI GGGAC 3 cut(s) 1141, 2715, 2867
BslI CCNNNNNNNGG 5 cut(s) 325, 348, 1540, 1811, 1885
BsmAI GTCTC 5 cut(s) 165, 509, 1138, 2319, 2464
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 3 cut(s) 1141, 2715, 2867
BsmI GAATGC 3 cut(s) 469, 1399, 2005
BsnI GGCC 3 cut(s) 1410, 2031, 2235
Bso31I GGTCTC 1 cut(s) 165
Bsp119I TTCGAA 2 cut(s) 190, 2382
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 1648
Bsp1720I GCTNAGC 1 cut(s) 2321
BspACI CCGC 8 cut(s) 82, 439, 623, 953, 1020, 1178, 1407, 1537
BspANI GGCC 3 cut(s) 1410, 2031, 2235
BspCNI CTCAG 9 cut(s) 739, 818, 1134, 1329, 1429, 2145, 2278, 2334, 2395
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 1 cut(s) 59
BspPI GGATC 8 cut(s) 310, 564, 620, 1421, 2134, 2172, 2528, 2792
BspT104I TTCGAA 2 cut(s) 190, 2382
BspTNI GGTCTC 1 cut(s) 165
BsrDI GCAATG 3 cut(s) 20, 1429, 1674
BsrFI RCCGGY 2 cut(s) 898, 1709
BsrGI TGTACA 1 cut(s) 1648
BsrI ACTGG 3 cut(s) 457, 1848, 2912
BssAI RCCGGY 2 cut(s) 898, 1709
BssECI CCNNGG 2 cut(s) 1440, 2823
BssNAI GTATAC 1 cut(s) 1282
BssNI GRCGYC 1 cut(s) 1749
BssT1I CCWWGG 2 cut(s) 1440, 2823
Bst1107I GTATAC 1 cut(s) 1282
Bst2UI CCWGG 2 cut(s) 766, 1883
Bst4CI ACNGT 9 cut(s) 196, 732, 892, 1381, 1495, 1648, 1702, 2694, 2798
Bst6I CTCTTC 6 cut(s) 281, 458, 1842, 2055, 2271, 2670
BstACI GRCGYC 1 cut(s) 1749
BstAUI TGTACA 1 cut(s) 1648
BstBI TTCGAA 2 cut(s) 190, 2382
BstC8I GCNNGC 5 cut(s) 621, 1155, 1860, 2122, 2718
BstEII GGTNACC 2 cut(s) 1186, 1712
BstENI CCTNNNNNAGG 1 cut(s) 1883
BstF5I GGATG 5 cut(s) 603, 877, 1450, 2509, 2516
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 2 cut(s) 86, 1858
BstMAI GTCTC 5 cut(s) 165, 509, 1138, 2319, 2464
BstMWI GCNNNNNNNGC 6 cut(s) 22, 81, 1310, 1319, 1901, 2042
BstNI CCWGG 2 cut(s) 766, 1883
BstNSI RCATGY 4 cut(s) 1009, 1862, 1908, 2230
BstPI GGTNACC 2 cut(s) 1186, 1712
BstSCI CCNGG 3 cut(s) 341, 764, 1881
BstSFI CTRYAG 1 cut(s) 1498
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 3 cut(s) 1812, 2356, 2819
BstV2I GAAGAC 4 cut(s) 650, 1496, 2169, 2460
BstX2I RGATCY 2 cut(s) 315, 2784
BstXI CCANNNNNNTGG 3 cut(s) 1787, 2011, 2830
BstYI RGATCY 2 cut(s) 315, 2784
BstZ17I GTATAC 1 cut(s) 1282
Bsu36I CCTNAGG 1 cut(s) 2780
BsuI GTATCC 1 cut(s) 2627
BsuRI GGCC 3 cut(s) 1410, 2031, 2235
BtgZI GCGATG 1 cut(s) 228
BtrI CACGTC 1 cut(s) 2458
BtsCI GGATG 5 cut(s) 603, 877, 1450, 2509, 2516
BtsI GCAGTG 1 cut(s) 1209
BtsIMutI CAGTG 5 cut(s) 464, 1209, 1334, 1386, 1644
Cac8I GCNNGC 5 cut(s) 621, 1155, 1860, 2122, 2718
CaiI CAGNNNCTG 2 cut(s) 1153, 1208
CfoI GCGC 2 cut(s) 86, 1858
Cfr10I RCCGGY 2 cut(s) 898, 1709
CseI GACGC 1 cut(s) 1738
Csp6I GTAC 6 cut(s) 369, 388, 1009, 1610, 1649, 2657
CspAI ACCGGT 1 cut(s) 1709
CspCI CAANNNNNGTGG 2 cut(s) 2113, 2148
CviQI GTAC 6 cut(s) 369, 388, 1009, 1610, 1649, 2657
DraI TTTAAA 2 cut(s) 1242, 2202
DriI GACNNNNNGTC 1 cut(s) 2650
EaeI YGGCCR 1 cut(s) 1408
Eam1104I CTCTTC 6 cut(s) 281, 458, 1842, 2055, 2271, 2670
Eam1105I GACNNNNNGTC 1 cut(s) 2650
EarI CTCTTC 6 cut(s) 281, 458, 1842, 2055, 2271, 2670
Ecl136II GAGCTC 1 cut(s) 225
Eco130I CCWWGG 2 cut(s) 1440, 2823
Eco24I GRGCYC 1 cut(s) 227
Eco31I GGTCTC 1 cut(s) 165
Eco32I GATATC 2 cut(s) 2159, 2622
Eco53kI GAGCTC 1 cut(s) 225
Eco57I CTGAAG 2 cut(s) 1703, 2730
Eco81I CCTNAGG 1 cut(s) 2780
Eco91I GGTNACC 2 cut(s) 1186, 1712
EcoICRI GAGCTC 1 cut(s) 225
EcoNI CCTNNNNNAGG 1 cut(s) 1883
EcoO65I GGTNACC 2 cut(s) 1186, 1712
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 764, 1881
EcoRV GATATC 2 cut(s) 2159, 2622
EcoT14I CCWWGG 2 cut(s) 1440, 2823
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 227
ErhI CCWWGG 2 cut(s) 1440, 2823
Esp3I CGTCTC 1 cut(s) 509
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 3 cut(s) 1141, 2715, 2867
FauI CCCGC 1 cut(s) 89
FblI GTMKAC 3 cut(s) 90, 338, 1281
Fnu4HI GCNGC 4 cut(s) 1408, 1826, 2370, 2808
FokI GGATG 5 cut(s) 610, 884, 1457, 2516, 2523
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 4 cut(s) 1408, 1826, 2370, 2808
FspBI CTAG 3 cut(s) 980, 2033, 2249
GlaI GCGC 2 cut(s) 85, 1857
GluI GCNGC 4 cut(s) 1408, 1826, 2370, 2808
GsaI CCCAGC 3 cut(s) 597, 1351, 2244
GsuI CTGGAG 1 cut(s) 1904
HaeIII GGCC 3 cut(s) 1410, 2031, 2235
HapII CCGG 8 cut(s) 145, 168, 343, 554, 648, 869, 899, 1710
HgaI GACGC 1 cut(s) 1738
HhaI GCGC 2 cut(s) 86, 1858
Hin1I GRCGYC 1 cut(s) 1749
Hin6I GCGC 2 cut(s) 84, 1856
HinP1I GCGC 2 cut(s) 84, 1856
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 5 cut(s) 73, 276, 814, 1681, 2714
HinfI GANTC 8 cut(s) 68, 413, 448, 778, 1223, 1776, 2334, 2565
HpaII CCGG 8 cut(s) 145, 168, 343, 554, 648, 869, 899, 1710
HphI GGTGA 7 cut(s) 373, 652, 863, 967, 1127, 1699, 2570
Hpy166II GTNNAC 7 cut(s) 91, 339, 1282, 1384, 2495, 2770, 2794
Hpy188III TCNNGA 8 cut(s) 407, 486, 526, 1470, 1733, 2162, 2348, 2878
Hpy8I GTNNAC 7 cut(s) 91, 339, 1282, 1384, 2495, 2770, 2794
HpyAV CCTTC 7 cut(s) 149, 1727, 1842, 1969, 2141, 2530, 2744
HpyCH4III ACNGT 9 cut(s) 196, 732, 892, 1381, 1495, 1648, 1702, 2694, 2798
HpyCH4IV ACGT 5 cut(s) 360, 518, 1130, 2298, 2457
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 81, 1310, 1319, 1901, 2042
HpySE526I ACGT 5 cut(s) 360, 518, 1130, 2298, 2457
Hsp92I GRCGYC 1 cut(s) 1749
HspAI GCGC 2 cut(s) 84, 1856
LmnI GCTCC 2 cut(s) 57, 1636
Lsp1109I GCAGC 3 cut(s) 1812, 2356, 2819
MaeI CTAG 3 cut(s) 980, 2033, 2249
MaeII ACGT 5 cut(s) 360, 518, 1130, 2298, 2457
MaeIII GTNAC 6 cut(s) 712, 886, 1186, 1256, 1712, 2798
MflI RGATCY 2 cut(s) 315, 2784
MhlI GDGCHC 1 cut(s) 227
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 3 cut(s) 948, 2281, 2448
Mph1103I ATGCAT 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 452
MslI CAYNNNNRTG 4 cut(s) 302, 1863, 2009, 2225
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 8 cut(s) 145, 168, 343, 554, 648, 869, 899, 1710
MspR9I CCNGG 3 cut(s) 343, 766, 1883
Mva1269I GAATGC 3 cut(s) 469, 1399, 2005
MvaI CCWGG 2 cut(s) 766, 1883
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 6 cut(s) 22, 81, 1310, 1319, 1901, 2042
NciI CCSGG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 59
NmeAIII GCCGAG 1 cut(s) 2261
NmuCI GTSAC 1 cut(s) 2798
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 4 cut(s) 1009, 1862, 1908, 2230
NspV TTCGAA 2 cut(s) 190, 2382
PaeI GCATGC 1 cut(s) 1862
PciI ACATGT 2 cut(s) 1005, 2226
PcsI WCGNNNNNNNCGW 3 cut(s) 58, 233, 498
PctI GAATGC 3 cut(s) 469, 1399, 2005
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 7 cut(s) 413, 448, 778, 1223, 1776, 2334, 2565
PflMI CCANNNNNTGG 1 cut(s) 325
PfoI TCCNGGA 2 cut(s) 764, 1881
PinAI ACCGGT 1 cut(s) 1709
PkrI GCNGC 4 cut(s) 1409, 1827, 2371, 2809
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
PscI ACATGT 2 cut(s) 1005, 2226
PsiI TTATAA 1 cut(s) 1565
Psp124BI GAGCTC 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 764, 1881
PspEI GGTNACC 2 cut(s) 1186, 1712
PspFI CCCAGC 3 cut(s) 593, 1347, 2240
PspGI CCWGG 2 cut(s) 764, 1881
PspN4I GGNNCC 1 cut(s) 59
PstNI CAGNNNCTG 2 cut(s) 1153, 1208
PsuI RGATCY 2 cut(s) 315, 2784
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 6 cut(s) 370, 389, 1010, 1611, 1650, 2658
RsaNI GTAC 6 cut(s) 369, 388, 1009, 1610, 1649, 2657
RseI CAYNNNNRTG 4 cut(s) 302, 1863, 2009, 2225
SacI GAGCTC 1 cut(s) 227
SalI GTCGAC 1 cut(s) 337
SatI GCNGC 4 cut(s) 1408, 1826, 2370, 2808
ScaI AGTACT 1 cut(s) 1611
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 343, 766, 1883
SduI GDGCHC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 1498
SfuI TTCGAA 2 cut(s) 190, 2382
SmiMI CAYNNNNRTG 4 cut(s) 302, 1863, 2009, 2225
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
SpeI ACTAGT 1 cut(s) 979
SphI GCATGC 1 cut(s) 1862
SsiI CCGC 8 cut(s) 82, 439, 623, 953, 1020, 1178, 1407, 1537
SspMI CTAG 3 cut(s) 980, 2033, 2249
SstI GAGCTC 1 cut(s) 227
StyD4I CCNGG 3 cut(s) 341, 764, 1881
StyI CCWWGG 2 cut(s) 1440, 2823
TaaI ACNGT 9 cut(s) 196, 732, 892, 1381, 1495, 1648, 1702, 2694, 2798
TaiI ACGT 5 cut(s) 363, 521, 1133, 2301, 2460
TatI WGTACW 5 cut(s) 368, 1008, 1609, 1648, 2656
TauI GCSGC 1 cut(s) 1410
TfiI GAWTC 7 cut(s) 413, 448, 778, 1223, 1776, 2334, 2565
TscAI CASTG 5 cut(s) 464, 1209, 1341, 1386, 1651
TseFI GTSAC 1 cut(s) 2798
TseI GCWGC 3 cut(s) 1825, 2369, 2807
Tsp45I GTSAC 1 cut(s) 2798
TspRI CASTG 5 cut(s) 464, 1209, 1341, 1386, 1651
Van91I CCANNNNNTGG 1 cut(s) 325
XagI CCTNNNNNAGG 1 cut(s) 1883
XapI RAATTY 5 cut(s) 203, 754, 1464, 2197, 2304
XceI RCATGY 4 cut(s) 1009, 1862, 1908, 2230
XcmI CCANNNNNNNNNTGG 3 cut(s) 206, 329, 1306
XmiI GTMKAC 3 cut(s) 90, 338, 1281
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 3 cut(s) 980, 2033, 2249
ZrmI AGTACT 1 cut(s) 1611
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.