RchiOBHm_Chr7g0183921

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016120
Physical Location & Seq
Reverse (-)
4643165 .. 4646229
3065 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16410

Sequence Viewer

Length: 2877 bp
ATGGCCTCTGTGGCACCACCACCACTCCCATTCTCATATCATCACCATCCCCTGCTTCCCAAACCAAACCCCAAACACTCCATTTTCACACCAAAATCTTCACTTTCTTTCTCTCCCGCTCAAACCAAAACCAACTCACTCCAAGACCTCAACCACCTCTGCAACTCCGGCAACCTCTCCGCCGCCCTCACCCTCCTCCAAACCAATGCCTTCCCCACCTCACAGCACCTCAAAGACGCCATCGGCGCCCTCCTCCAGGCCTGTGGCCGCCACAAGGACATCGAAACCGGCCGCAAGCTCCACCACCTCGTCTCCCAATCCACCCACTTCACCCACGACTTTGTCCTCAACACCCGCATCATCACCATGTACTCAATGTGCAACTCTCCTTCCGATTCCCGCCTCGTTTTCGAAGGGTTACAGAGAAAGAACCTGTTCCAGTGGAATGCTCTGGTTAGTGGGTACTCAAGAAACGAGCTTTATGAGGAAGCAATTGATACCTTCATTAAGTTGATAAGCGCCGCGGAGTTTAAACCCGATAATTTCACAATGCCTTGTGTGATTAAGGCCTGTGGTGGGCTTTTGGATGTGGGTTTGGGGCAGGGGGTTCATGGGATGGTTGTGAAGATGGGGCTGGTTTCGGATGTGTTTGTGGGGAATGCATTGATTGCGGTGTATGGGAGATTTGGGATTGTTGGAGATTCAGTTAAGGTGTTTGAGAAAATGAGTGCGAGGAACTTGGTGTCTTGGAATTCGATGATTGGGGGGTTTTCGGAGAATGGGATGTGTGAAGAGAGTTGTAGGTTGTTGGTGAGGCTTTTGGAGGCTGAGGAAGGGTTTGTTCCGGATGAGGCGACGTTGGTGACTGTATTACCTGTTTGTGCTGGGAAAGGGGATGTTAGAATGGGGATGGAGATTCATGGTTTGGCGGTGAAGTTGGGGCTGGATAAGGAGTTGATGTTGAGTAATGCCTTGATGGACATGTATTTGAAATGTGGGTACTTAACGGAAGCTCGAGTTTTGTTTGAGAGAAATGAGAGTAAGAATGTGGTGTCTTGGAATGCTTTCATAGGAGGTTATTCTAGACAAGGGGATGTTAGTGGAGCATTTGATCTTTTGCAAAAAATGCAGATGAAGGAGAAGGTGAATGAGGTCACCCTCTTAAATGTGTTACCGGTTTTTTTAGAGGAGTCGGAACTTTTGAGCTTGAAAGAGCTTCATGGTTACTCATTCAGACATGGGTTTCAAGATGATGAGCTGGTAGCCAATGCTTTTGTTGCAGCCTATGCGAAATGTGGGTCACTGAGTTCTGCTGAGCAGGTGTTTTATGGTATCGAGACGAAGACAGTGAGCTCCTGGAATGCAGTTATTGGTGGCCATGCCCAGAATGGAGATCCAAAAAAGGCTTTAGATTTGTACCTTCAAATGAAACAATCAGGTTTGGATCCTGACAAGTTTAGCATTGGTAGCCTCCTTTTAGCTTGTGCCCACCTCAAATTTCTTCAATATGGTAAAGAGATTCATGGGTTTGTGCTACGTAATGGGTTAGAGTTGGATTCATTTATTGGTATTTCATTACTGTCCTTTTACATCCAATGTGGTAAACTATCGACGGCAAGAGCATTATTTGATAGGATGGAAGATAAAATTCCAGTGTCATGGAATGCAATAATCTCTGGTTACTCTCAGATTGGACTTCCTGATGAAGCCCTCGATCTCTTTCGACTAATGCTGTCTAATGGAATCCTACCTGGTGAAATAGCCACAATGAGTGCACTGGGGGCTTGTTCACAACTGTCAGCTCTGCGTCTAGGGAAAGAACTGCATTGTTTTGCTTTGAAAGCACGCCTTACACTAGACCTGTTTGTTGGTTGTTCTCTCATAGATATGTATGCAAAAAGTGGCTGCATAGAACAATCTCACAGGGCATTTGACAGTTTAACAAAGAAAGATGTGGCATCATGGAATGTTATAATTGCAGGGTATGGAATTCATGGACATGGAAACAAGGCTTTGGAGCTATTTGGAGAAATGATAAGTTTGGGGCATAAGCCTGATGTCTTTACATTTCTTGGAATTCTAACTGCATGTAACCATGCTGGGCTTGTTAAAAATGGGCTAAAATATTTCAAAGAGATGCAAAGTTTATATGGAATAGAGCCGAAGTTAGAGCATTATGCATGTGTAGTAGACATGCTGGGTAGGGCTGGTCAATTGGAGGAAGCTCTAAACCTGATAGATAACATGCCTGAAGAACCAGATACTAGAATTTGGAGTTCATTGCTTAGTTCCTGTAGGAATTATAATGATTTGGGAATGGGACAGAAAATTGCTGAAAAATTACTAGAATTGGAGCCCGGAAAGGCAGAGAATTATGTCCTACTGTCGAACTTATATGCTGCATCAGGGAATTGGGATGATGTGAGATGGGTCCGGCAAAGGATGAAGGAGATTGGCCTTCAGAAAGAAGCTGGCCGCAGTTGGATTGAAATTGGAGGGCAAGTGTATAACTTTGTTGCTGGTGACAATTCTCTGCCAGAGTCGGCGGAGATTAGGAAGATGTGGACAAAAGTAGAGGAAAGAATAAGTAAACTCGGATATACACCCAACACAGATTCTGTGCTTCATGAAGTGGACTATACAGAGAAGATTGAAATATTGAGGGGGCATAGTGAAAAGCTTGCAATTTCATTTGGATTGTTAAAGATGAAAAAAGGTGCTACACTGAGAGTCTGTAAAAATCTGCGCATCTGCATTGATTGTCACAATGCTGCTAAGTTGATATCGAAGGCTGTTGAAACGGAGATAATTGTGAGAGACAACAAGCGCTTCCATCATTTCAAAGATGGCCTCTGTTCTTGTGGAGATTATTGGTAA

Protein Analysis

958

Amino Acids

106.09

Weight (kDa)

6.45

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 348 - 379 1.5e-06 PPR repeat family
PPR_2 PF13041 449 - 497 2.5e-10 PPR repeat family
PPR PF01535 452 - 481 2.8e-07 PPR repeat
PPR PF01535 552 - 582 7.4e-09 PPR repeat
PPR_2 PF13041 552 - 586 2.3e-06 PPR repeat family
PPR_2 PF13041 650 - 697 4.6e-11 PPR repeat family
PPR PF01535 654 - 682 2.6e-06 PPR repeat
E_motif PF20431 768 - 830 1.3e-17 E motif
DYW_deaminase PF14432 866 - 958 2e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18485
fragaria_vesca FvH4_5g13240
malus_domestica MD06G1207000.v1.1
prunus_persica Prupe.5G211400_v2.0.a1
pyrus_communis pycom06g18480
rosa_chinensis RchiOBHm_Chr7g0183921
rosa_laevigata RLG00000005010
rosa_multiflora Rmu_sc0004712.1_g000029 Rmu_sc0007533.1_g000009
rosa_rugosa Rorug06G0461400
rosa_samantha Rh7AG062600 Rh7BG062800 Rh7CG064100 Rh7DG062600
rosa_wichuraiana Rw7G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1973, 2304
AarI CACCTGC 1 cut(s) 1309
Acc16I TGCGCA 1 cut(s) 2747
Acc36I ACCTGC 1 cut(s) 1309
AccB1I GGYRCC 2 cut(s) 13, 245
AccBSI CCGCTC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 2190
AccII CGCG 1 cut(s) 524
AccIII TCCGGA 1 cut(s) 844
AclWI GGATC 3 cut(s) 1388, 1439, 1452
AcoI YGGCCR 4 cut(s) 265, 289, 1375, 2473
AcsI RAATTY 6 cut(s) 751, 1496, 1647, 1989, 2076, 2268
AcuI CTGAAG 2 cut(s) 2271, 2444
AcyI GRCGYC 2 cut(s) 237, 246
AfaI GTAC 4 cut(s) 371, 464, 1001, 1418
AfeI AGCGCT 1 cut(s) 2828
AfiI CCNNNNNNNGG 3 cut(s) 256, 274, 576
AflIII ACRYGT 1 cut(s) 981
AgeI ACCGGT 1 cut(s) 1174
AjnI CCWGG 3 cut(s) 255, 1355, 1750
Alw21I GWGCWC 2 cut(s) 1355, 1777
Alw26I GTCTC 3 cut(s) 316, 1331, 2811
Alw44I GTGCAC 1 cut(s) 1773
AlwI GGATC 3 cut(s) 1388, 1439, 1452
AlwNI CAGNNNCTG 1 cut(s) 2618
Ama87I CYCGRG 1 cut(s) 1014
Aor13HI TCCGGA 1 cut(s) 844
Aor51HI AGCGCT 1 cut(s) 2828
AoxI GGCC 9 cut(s) 3, 258, 265, 289, 567, 1375, 2455, 2473, 2848
ApaLI GTGCAC 1 cut(s) 1773
ApeKI GCWGC 4 cut(s) 1280, 1905, 2399, 2771
ApoI RAATTY 6 cut(s) 751, 1496, 1647, 1989, 2076, 2268
ArsI GACNNNNNNTTYG 2 cut(s) 1283, 1315
AsiGI ACCGGT 1 cut(s) 1174
Asp700I GAANNNNTTC 2 cut(s) 434, 1064
AspLEI GCGC 4 cut(s) 248, 521, 2748, 2829
AspS9I GGNCC 1 cut(s) 2431
AsuC2I CCSGG 1 cut(s) 2358
AsuII TTCGAA 1 cut(s) 411
AvaI CYCGRG 1 cut(s) 1014
AvaII GGWCC 1 cut(s) 2431
BaeGI GKGCMC 2 cut(s) 1489, 1777
BalI TGGCCA 1 cut(s) 1377
BamHI GGATCC 1 cut(s) 1444
BanI GGYRCC 2 cut(s) 13, 245
BanII GRGCYC 2 cut(s) 1355, 2358
BbsI GAAGAC 1 cut(s) 1349
Bbv12I GWGCWC 2 cut(s) 1355, 1777
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 4 cut(s) 1292, 1892, 2386, 2758
BceAI ACGGC 1 cut(s) 1629
BciT130I CCWGG 3 cut(s) 257, 1357, 1752
BcnI CCSGG 1 cut(s) 2358
BcoDI GTCTC 3 cut(s) 316, 1331, 2811
BfaI CTAG 5 cut(s) 1083, 1811, 1856, 2265, 2345
BfmI CTRYAG 1 cut(s) 2293
BfoI RGCGCY 3 cut(s) 249, 522, 2830
BfuAI ACCTGC 1 cut(s) 1309
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 9 cut(s) 183, 268, 292, 522, 1281, 1906, 2400, 2476, 2772
BlpI GCTNAGC 1 cut(s) 1314
BlsI GCNGC 9 cut(s) 184, 269, 293, 523, 1282, 1907, 2401, 2477, 2773
Bme1390I CCNGG 4 cut(s) 257, 1357, 1752, 2358
Bme18I GGWCC 1 cut(s) 2431
BmeT110I CYCGRG 1 cut(s) 1014
BmgT120I GGNCC 1 cut(s) 2431
BmiI GGNNCC 5 cut(s) 15, 247, 1446, 2355, 2432
BmrFI CCNGG 4 cut(s) 257, 1357, 1752, 2358
BmrI ACTGGG 1 cut(s) 1787
BmsI GCATC 5 cut(s) 366, 1967, 2127, 2411, 2757
BmuI ACTGGG 1 cut(s) 1787
BpiI GAAGAC 1 cut(s) 1349
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 828
Bpu1102I GCTNAGC 1 cut(s) 1314
Bpu14I TTCGAA 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 2358
BsaAI YACGTR 1 cut(s) 1538
BsaHI GRCGYC 2 cut(s) 237, 246
BsaJI CCNNGG 1 cut(s) 522
BsaWI WCCGGW 2 cut(s) 844, 1174
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 274, 576
Bse118I RCCGGY 2 cut(s) 287, 1174
Bse1I ACTGG 3 cut(s) 439, 1652, 1782
Bse3DI GCAATG 1 cut(s) 2279
BseAI TCCGGA 1 cut(s) 844
BseBI CCWGG 3 cut(s) 257, 1357, 1752
BseDI CCNNGG 1 cut(s) 522
BseLI CCNNNNNNNGG 3 cut(s) 256, 274, 576
BseMI GCAATG 1 cut(s) 2279
BseMII CTCAG 5 cut(s) 819, 1295, 1305, 1700, 2717
BseNI ACTGG 3 cut(s) 439, 1652, 1782
BseRI GAGGAG 3 cut(s) 185, 242, 1202
BseSI GKGCMC 2 cut(s) 1489, 1777
BseX3I CGGCCG 1 cut(s) 289
BseXI GCAGC 4 cut(s) 1292, 1892, 2386, 2758
BseYI CCCAGC 3 cut(s) 884, 2099, 2197
Bsh1236I CGCG 1 cut(s) 524
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 9 cut(s) 5, 260, 267, 291, 569, 1377, 2457, 2475, 2850
BshNI GGYRCC 2 cut(s) 13, 245
BshTI ACCGGT 1 cut(s) 1174
BsiEI CGRYCG 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 1355, 1777
BsiHKCI CYCGRG 1 cut(s) 1014
BsiSI CCGG 6 cut(s) 168, 288, 845, 1175, 2358, 2434
BslFI GGGAC 1 cut(s) 2334
BslI CCNNNNNNNGG 3 cut(s) 256, 274, 576
BsmAI GTCTC 3 cut(s) 316, 1331, 2811
BsmBI CGTCTC 2 cut(s) 316, 1331
BsmFI GGGAC 1 cut(s) 2334
BsmI GAATGC 5 cut(s) 451, 664, 1066, 1366, 1669
BsnI GGCC 9 cut(s) 5, 260, 267, 291, 569, 1377, 2457, 2475, 2850
BsoBI CYCGRG 1 cut(s) 1014
Bsp119I TTCGAA 1 cut(s) 411
Bsp1286I GDGCHC 4 cut(s) 1355, 1489, 1777, 2358
Bsp13I TCCGGA 1 cut(s) 844
Bsp143I GATC 4 cut(s) 1111, 1393, 1444, 1714
Bsp1720I GCTNAGC 1 cut(s) 1314
BspANI GGCC 9 cut(s) 5, 260, 267, 291, 569, 1377, 2457, 2475, 2850
BspCNI CTCAG 5 cut(s) 820, 1296, 1306, 1699, 2718
BspEI TCCGGA 1 cut(s) 844
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 1 cut(s) 2626
BspLI GGNNCC 5 cut(s) 15, 247, 1446, 2355, 2432
BspMI ACCTGC 1 cut(s) 1309
BspPI GGATC 3 cut(s) 1388, 1439, 1452
BspT104I TTCGAA 1 cut(s) 411
BspT107I GGYRCC 2 cut(s) 13, 245
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 2279
BsrFI RCCGGY 2 cut(s) 287, 1174
BsrI ACTGG 3 cut(s) 439, 1652, 1782
BssAI RCCGGY 2 cut(s) 287, 1174
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 4 cut(s) 1111, 1393, 1444, 1714
BssNI GRCGYC 2 cut(s) 237, 246
Bst2UI CCWGG 3 cut(s) 257, 1357, 1752
Bst4CI ACNGT 6 cut(s) 868, 1348, 1581, 1797, 1937, 2385
Bst6I CTCTTC 1 cut(s) 786
BstACI GRCGYC 2 cut(s) 237, 246
BstAPI GCANNNNNTGC 3 cut(s) 668, 1126, 1286
BstBAI YACGTR 1 cut(s) 1538
BstBI TTCGAA 1 cut(s) 411
BstC8I GCNNGC 4 cut(s) 296, 1846, 2473, 2682
BstDEI CTNAG 7 cut(s) 828, 1304, 1314, 1686, 2285, 2726, 2775
BstDSI CCRYGG 1 cut(s) 522
BstEII GGTNACC 1 cut(s) 1153
BstENI CCTNNNNNAGG 1 cut(s) 254
BstFNI CGCG 1 cut(s) 524
BstH2I RGCGCY 3 cut(s) 249, 522, 2830
BstHHI GCGC 4 cut(s) 248, 521, 2748, 2829
BstKTI GATC 4 cut(s) 1114, 1396, 1447, 1717
BstMAI GTCTC 3 cut(s) 316, 1331, 2811
BstMBI GATC 4 cut(s) 1111, 1393, 1444, 1714
BstMCI CGRYCG 1 cut(s) 292
BstNI CCWGG 3 cut(s) 257, 1357, 1752
BstNSI RCATGY 5 cut(s) 985, 2091, 2184, 2197, 2248
BstPI GGTNACC 1 cut(s) 1153
BstSCI CCNGG 4 cut(s) 255, 1355, 1750, 2356
BstSFI CTRYAG 1 cut(s) 2293
BstSLI GKGCMC 2 cut(s) 1489, 1777
BstSNI TACGTA 1 cut(s) 1538
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 4 cut(s) 1292, 1892, 2386, 2758
BstV2I GAAGAC 1 cut(s) 1349
BstX2I RGATCY 2 cut(s) 1393, 1444
BstXI CCANNNNNNTGG 2 cut(s) 263, 1659
BstYI RGATCY 2 cut(s) 1393, 1444
BstZI CGGCCG 1 cut(s) 289
BsuRI GGCC 9 cut(s) 5, 260, 267, 291, 569, 1377, 2457, 2475, 2850
BtgI CCRYGG 1 cut(s) 522
BtsIMutI CAGTG 6 cut(s) 446, 1301, 1353, 1659, 1775, 2723
BveI ACCTGC 1 cut(s) 1309
Cac8I GCNNGC 4 cut(s) 296, 1846, 2473, 2682
CaiI CAGNNNCTG 1 cut(s) 2618
CciI TCATGA 1 cut(s) 2626
CfoI GCGC 4 cut(s) 248, 521, 2748, 2829
Cfr10I RCCGGY 2 cut(s) 287, 1174
Cfr13I GGNCC 1 cut(s) 2431
Cfr42I CCGCGG 1 cut(s) 525
CseI GACGC 2 cut(s) 245, 1796
CsiI ACCWGGT 1 cut(s) 1750
Csp6I GTAC 4 cut(s) 370, 463, 1000, 1417
CspAI ACCGGT 1 cut(s) 1174
CviQI GTAC 4 cut(s) 370, 463, 1000, 1417
DdeI CTNAG 7 cut(s) 828, 1304, 1314, 1686, 2285, 2726, 2775
DinI GGCGCC 1 cut(s) 247
DpnI GATC 4 cut(s) 1113, 1395, 1446, 1716
DpnII GATC 4 cut(s) 1111, 1393, 1444, 1714
DraI TTTAAA 1 cut(s) 532
EaeI YGGCCR 4 cut(s) 265, 289, 1375, 2473
EagI CGGCCG 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 2 cut(s) 169, 2561
Ecl136II GAGCTC 1 cut(s) 1353
EclXI CGGCCG 1 cut(s) 289
Eco105I TACGTA 1 cut(s) 1538
Eco147I AGGCCT 2 cut(s) 260, 569
Eco24I GRGCYC 2 cut(s) 1355, 2358
Eco32I GATATC 1 cut(s) 2784
Eco47I GGWCC 1 cut(s) 2431
Eco47III AGCGCT 1 cut(s) 2828
Eco52I CGGCCG 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 1353
Eco57I CTGAAG 2 cut(s) 2271, 2444
Eco88I CYCGRG 1 cut(s) 1014
Eco91I GGTNACC 1 cut(s) 1153
EcoICRI GAGCTC 1 cut(s) 1353
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 1153
EcoRI GAATTC 3 cut(s) 751, 1989, 2076
EcoRII CCWGG 3 cut(s) 255, 1355, 1750
EcoRV GATATC 1 cut(s) 2784
EcoT22I ATGCAT 2 cut(s) 664, 2182
EcoT38I GRGCYC 2 cut(s) 1355, 2358
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
Esp3I CGTCTC 2 cut(s) 316, 1331
FalI AAGNNNNNCTT 2 cut(s) 1833, 1865
FaqI GGGAC 1 cut(s) 2334
FauI CCCGC 3 cut(s) 124, 362, 407
FblI GTMKAC 1 cut(s) 2190
Fnu4HI GCNGC 9 cut(s) 183, 268, 292, 522, 1281, 1906, 2400, 2476, 2772
FriOI GRGCYC 2 cut(s) 1355, 2358
Fsp4HI GCNGC 9 cut(s) 183, 268, 292, 522, 1281, 1906, 2400, 2476, 2772
FspBI CTAG 5 cut(s) 1083, 1811, 1856, 2265, 2345
FspI TGCGCA 1 cut(s) 2747
GlaI GCGC 4 cut(s) 247, 520, 2747, 2828
GluI GCNGC 9 cut(s) 183, 268, 292, 522, 1281, 1906, 2400, 2476, 2772
GsaI CCCAGC 3 cut(s) 888, 2103, 2201
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 3 cut(s) 249, 522, 2830
HaeIII GGCC 9 cut(s) 5, 260, 267, 291, 569, 1377, 2457, 2475, 2850
HapII CCGG 6 cut(s) 168, 288, 845, 1175, 2358, 2434
HgaI GACGC 2 cut(s) 245, 1796
HhaI GCGC 4 cut(s) 248, 521, 2748, 2829
Hin1I GRCGYC 2 cut(s) 237, 246
Hin6I GCGC 4 cut(s) 246, 519, 2746, 2827
HinP1I GCGC 4 cut(s) 246, 519, 2746, 2827
HindIII AAGCTT 1 cut(s) 2678
HpaII CCGG 6 cut(s) 168, 288, 845, 1175, 2358, 2434
Hpy166II GTNNAC 7 cut(s) 1604, 1775, 1790, 2191, 2565, 2591, 2635
Hpy188I TCNGA 8 cut(s) 394, 643, 775, 1195, 1235, 1689, 2463, 2597
Hpy188III TCNNGA 8 cut(s) 468, 845, 1083, 1247, 1336, 1448, 1700, 2627
Hpy8I GTNNAC 7 cut(s) 1604, 1775, 1790, 2191, 2565, 2591, 2635
Hpy99I CGWCG 2 cut(s) 859, 1615
HpyCH4III ACNGT 6 cut(s) 868, 1348, 1581, 1797, 1937, 2385
HpyCH4IV ACGT 2 cut(s) 857, 1537
HpyF3I CTNAG 7 cut(s) 828, 1304, 1314, 1686, 2285, 2726, 2775
HpySE526I ACGT 2 cut(s) 857, 1537
Hsp92I GRCGYC 2 cut(s) 237, 246
HspAI GCGC 4 cut(s) 246, 519, 2746, 2827
KasI GGCGCC 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 844
KspI CCGCGG 1 cut(s) 525
Kzo9I GATC 4 cut(s) 1111, 1393, 1444, 1714
LmnI GCTCC 5 cut(s) 303, 1103, 1358, 2017, 2353
Lsp1109I GCAGC 4 cut(s) 1292, 1892, 2386, 2758
LweI GCATC 5 cut(s) 366, 1967, 2127, 2411, 2757
MabI ACCWGGT 1 cut(s) 1750
MaeI CTAG 5 cut(s) 1083, 1811, 1856, 2265, 2345
MaeII ACGT 2 cut(s) 857, 1537
MalI GATC 4 cut(s) 1113, 1395, 1446, 1716
MbiI CCGCTC 1 cut(s) 119
MboI GATC 4 cut(s) 1111, 1393, 1444, 1714
MboII GAAGA 9 cut(s) 90, 637, 803, 1354, 1493, 1652, 2264, 2569, 2659
MfeI CAATTG 2 cut(s) 492, 2213
MflI RGATCY 2 cut(s) 1393, 1444
MhlI GDGCHC 4 cut(s) 1355, 1489, 1777, 2358
MlsI TGGCCA 1 cut(s) 1377
MluNI TGGCCA 1 cut(s) 1377
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 1199, 2549, 2739
MmeI TCCRAC 4 cut(s) 676, 1173, 1533, 2462
Mox20I TGGCCA 1 cut(s) 1377
Mph1103I ATGCAT 2 cut(s) 664, 2182
MroI TCCGGA 1 cut(s) 844
MroXI GAANNNNTTC 2 cut(s) 434, 1064
MscI TGGCCA 1 cut(s) 1377
MseI TTAA 9 cut(s) 507, 531, 564, 708, 1004, 1163, 1940, 2109, 2702
MslI CAYNNNNRTG 3 cut(s) 365, 1886, 1998
Msp20I TGGCCA 1 cut(s) 1377
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 6 cut(s) 168, 288, 845, 1175, 2358, 2434
MspR9I CCNGG 4 cut(s) 257, 1357, 1752, 2358
MssI GTTTAAAC 1 cut(s) 532
MunI CAATTG 2 cut(s) 492, 2213
Mva1269I GAATGC 5 cut(s) 451, 664, 1066, 1366, 1669
MvaI CCWGG 3 cut(s) 257, 1357, 1752
MvnI CGCG 1 cut(s) 524
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 2358
NdeII GATC 4 cut(s) 1111, 1393, 1444, 1714
NlaIV GGNNCC 5 cut(s) 15, 247, 1446, 2355, 2432
NmuCI GTSAC 5 cut(s) 862, 1153, 1299, 2522, 2762
NsbI TGCGCA 1 cut(s) 2747
NsiI ATGCAT 2 cut(s) 664, 2182
NspI RCATGY 5 cut(s) 985, 2091, 2184, 2197, 2248
NspV TTCGAA 1 cut(s) 411
PaeR7I CTCGAG 1 cut(s) 1014
PagI TCATGA 1 cut(s) 2626
PaqCI CACCTGC 1 cut(s) 1309
PceI AGGCCT 2 cut(s) 260, 569
PciI ACATGT 1 cut(s) 981
PctI GAATGC 5 cut(s) 451, 664, 1066, 1366, 1669
PdmI GAANNNNTTC 2 cut(s) 434, 1064
PfeI GAWTC 7 cut(s) 395, 701, 916, 1519, 1556, 1743, 2615
PflFI GACNNNGTC 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 1355
PinAI ACCGGT 1 cut(s) 1174
PkrI GCNGC 9 cut(s) 184, 269, 293, 523, 1282, 1907, 2401, 2477, 2773
PleI GAGTC 3 cut(s) 1198, 2548, 2738
PluTI GGCGCC 1 cut(s) 249
PmeI GTTTAAAC 1 cut(s) 532
PpsI GAGTC 3 cut(s) 1198, 2548, 2738
Ppu21I YACGTR 1 cut(s) 1538
PscI ACATGT 1 cut(s) 981
PsiI TTATAA 2 cut(s) 1973, 2304
Psp124BI GAGCTC 1 cut(s) 1355
Psp6I CCWGG 3 cut(s) 255, 1355, 1750
PspEI GGTNACC 1 cut(s) 1153
PspFI CCCAGC 3 cut(s) 884, 2099, 2197
PspGI CCWGG 3 cut(s) 255, 1355, 1750
PspN4I GGNNCC 5 cut(s) 15, 247, 1446, 2355, 2432
PspPI GGNCC 1 cut(s) 2431
PspXI VCTCGAGB 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 2618
PsuI RGATCY 2 cut(s) 1393, 1444
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 4 cut(s) 371, 464, 1001, 1418
RsaNI GTAC 4 cut(s) 370, 463, 1000, 1417
RseI CAYNNNNRTG 3 cut(s) 365, 1886, 1998
SacI GAGCTC 1 cut(s) 1355
SacII CCGCGG 1 cut(s) 525
SaqAI TTAA 9 cut(s) 507, 531, 564, 708, 1004, 1163, 1940, 2109, 2702
SatI GCNGC 9 cut(s) 183, 268, 292, 522, 1281, 1906, 2400, 2476, 2772
Sau3AI GATC 4 cut(s) 1111, 1393, 1444, 1714
Sau96I GGNCC 1 cut(s) 2431
SchI GAGTC 3 cut(s) 1199, 2549, 2739
ScrFI CCNGG 4 cut(s) 257, 1357, 1752, 2358
SduI GDGCHC 4 cut(s) 1355, 1489, 1777, 2358
SexAI ACCWGGT 1 cut(s) 1750
SfaNI GCATC 5 cut(s) 366, 1967, 2127, 2411, 2757
SfcI CTRYAG 1 cut(s) 2293
SfoI GGCGCC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 1014
Sfr303I CCGCGG 1 cut(s) 525
SfuI TTCGAA 1 cut(s) 411
SgrBI CCGCGG 1 cut(s) 525
SinI GGWCC 1 cut(s) 2431
SlaI CTCGAG 1 cut(s) 1014
SmiMI CAYNNNNRTG 3 cut(s) 365, 1886, 1998
SmlI CTYRAG 2 cut(s) 466, 1014
SmoI CTYRAG 2 cut(s) 466, 1014
SnaBI TACGTA 1 cut(s) 1538
SseBI AGGCCT 2 cut(s) 260, 569
SspDI GGCGCC 1 cut(s) 245
SspI AATATT 2 cut(s) 2126, 2658
SspMI CTAG 5 cut(s) 1083, 1811, 1856, 2265, 2345
SstI GAGCTC 1 cut(s) 1355
StuI AGGCCT 2 cut(s) 260, 569
StyD4I CCNGG 4 cut(s) 255, 1355, 1750, 2356
TaaI ACNGT 6 cut(s) 868, 1348, 1581, 1797, 1937, 2385
TaiI ACGT 2 cut(s) 860, 1540
TatI WGTACW 1 cut(s) 369
TauI GCSGC 5 cut(s) 185, 270, 294, 524, 2478
TfiI GAWTC 7 cut(s) 395, 701, 916, 1519, 1556, 1743, 2615
Tru1I TTAA 9 cut(s) 507, 531, 564, 708, 1004, 1163, 1940, 2109, 2702
Tru9I TTAA 9 cut(s) 507, 531, 564, 708, 1004, 1163, 1940, 2109, 2702
TscAI CASTG 6 cut(s) 446, 1308, 1353, 1659, 1782, 2730
TseFI GTSAC 5 cut(s) 862, 1153, 1299, 2522, 2762
TseI GCWGC 4 cut(s) 1280, 1905, 2399, 2771
Tsp45I GTSAC 5 cut(s) 862, 1153, 1299, 2522, 2762
TspGWI ACGGA 2 cut(s) 1022, 2816
TspRI CASTG 6 cut(s) 446, 1308, 1353, 1659, 1782, 2730
Tth111I GACNNNGTC 1 cut(s) 341
VneI GTGCAC 1 cut(s) 1773
VpaK11BI GGWCC 1 cut(s) 2431
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 6 cut(s) 751, 1496, 1647, 1989, 2076, 2268
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 5 cut(s) 985, 2091, 2184, 2197, 2248
XcmI CCANNNNNNNNNTGG 1 cut(s) 1385
XhoI CTCGAG 1 cut(s) 1014
XmiI GTMKAC 1 cut(s) 2190
XmnI GAANNNNTTC 2 cut(s) 434, 1064
XspI CTAG 5 cut(s) 1083, 1811, 1856, 2265, 2345
Zsp2I ATGCAT 2 cut(s) 664, 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.