Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0016120

Physical Location & Seq

Genomic Coordinates Reverse (-)

34164129 .. 34167059

Gene Structure 2931 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1207000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGGCTTCTGTGGCAGTGGCACCACCACCACTCTCATGCCATTCTCTGCCTACACCTAACGTCAAACACTTCCTCCGCCCAATCTTCCCTCCAAAACCAAAACCACTTTCTTCTCTCTCCGCCGCACAGACAACCACCCACCACTACCACCAAACAGACTCTCTCACTTTCCTCCAAGACGTCAACAATCTCTGTGACGCCGGAAACCTCTCTGAGGCTCTGACCCTTCTTCGAGCACACTCTCACGATGCAGTTTCCAGCTCACAGCAAGGGAAGGACGCCATGGGAACCTTGTTACAGGCCTGCGGGCGCCGCAAGGACGTCGAAACCGGACGCAAAGTCCACAACTTGGTCTCTGCGTCGACCGTTTTCAGCAGTGACTTTGTTCTCAACACACGCATCATTACCATGTATGCAATGTGCGGTTCTCCTTTGGATTCCCGCTCAGTTTTCGACGGGCTGAAGAGGAAGAACTTGTTCCAGTGGAATGCGCTTGTTAGTGGGTATGCAAGAAACGAGCTTTTTGTTGATGCCATTGATCTGTTTGTTGAGTTGATCAGCGTTACGGAGTTTAAACCCGATAATTTTACTTTTCCCTGTGTTTTTAAGGCCTGTGGGGGGATTTCGGACGTGGGTTTGGGACAGGTTGTTCATGGGATGGCTGTGAAGATGGGATTGATTTCGGATGTGTTTGTGGGCAATGCGTTGATTGCGATGTATGGGAAATGCGGGTCGGTTGAGGATGCAGCTAAAATGTTCGAGATAATGCCTGAGAAGAATTTGGTTTCTTGGAATTCGATGATATGCGGGTTCTCGGAGAATGGATTGGATCATGAGAGTTATAGTTTGTTGGGGAAGATTTTAGAGAGTGAAGAAGCTCTAGTACCGGATGTTGCTACTTTGGTGACTGTATTGCCTTTGTGTGCTGGGAACGGAGAAGTAAATATGGGGATGATGATTCATAGTTTGGCAGTGAAATTGGGGCTGAACCAGGAATTAATGGTGAATAATGCGTTGGCGGACATGTACTTGAAATGTGGGTACTCAGTTGAAGCTCAAGTTTTGTTTGACAAGAATGACAAGAAAAATGTGGTTTCTTGGAATTCCGTCATAGGAGGTTTTTCTAGAGAAGGGGATGTATGTGGCACATTTGATCTTTTGCGAAAAATGCAGATGGAAGAGGAGAAAGTGAAGGTGAATGAGGTCACCATACTGAACGTGTTACCGGCTTGTTTGGAGGAGTCGGAACTTTTGAGCTTGAAAGAACTTCATGCTTATTCATTCAGACATTGGTTTATATATGATGAACTGGTAGCCAATGCTTTTGTTGCAGCGTACACGAAATGTGGGTCACTAAATTCCGCTGAGCTTGTTTTTCATGGTATTGAGACGAAGACAGTGGGCTCCTGGAACGCAGTTATTGGTGGCTGTGCCCAAAATGGAGATCCATACAAAGCTTTAGATTTGTACCTTCAAATGAAATATTCAGGCTTGGATCCTGATGAATTTAGCATCGGTAGCCTCCTTTTAGCTTGTGCCCACCTGAAACATCTTCAACACGGGAGAGAGATTCATGGATTTGTGCTGCGAAATGGGTTAGAAATGGATTCATTCATTGGTATTTCATTACTATCCGTTTACATCCATTGCGGAAAATTATCATCTGCAAGAATATTATTTGATAGGACAGAATCAAAAATTAGTGTGTCTTGGAATGCAATGATTGCCGGTTACACTCAGGTTGGACTTCCTGACAAAGCCCTTGATCTCTTTCGCCAAATGCTGTCTGATGAAATCCTGCCTTGTGAAATTGCAACAATGAGCATGTTTGGGGCTTGTTCGCAACTGTCAGCTTTGCGTTCAGGAAAAGAGCTGCATTGTTTCGCTTTGAAAGCTCGCCTCACAGAAGACCTTTTTGTTGGTTGCTCTCTCATAGATATGTATGCAAAAAGTGGCTGCATCGAACAATCTCATAGAGTTTTTGACCGGTTAACCAAGAAGGATGTGCCTTCATGGAACGTTATAATTGCAGGATATGGAATTCATGGACATGGAAACAAGGCTTTAGAGCTCTTCAGAGAAATGCTAAGTTTTGGGCAGAAGCCTGATGGGTTTACGTTTATCGGAATTTTAACTGCATGTAGCCATGCCGGGTTGGTTAAGGAGGGGATAGAATATTTCAACCAGATGCAAAGCTTGTACAAAATAGAGCCGAAACTAGAGCATTATGCATGTGTGGTGGACATGCTTGGCCGGGCTGGCCGGTTGGAGGAAGCTTTAAACCTCATACATGAGATGCCGGAGGAGCCAGATACTAGAATGTGGAGCTCATTGCTTAGTTCCTGTAGGAGTCATAATAATTTGGACATGGGGCAGAAAATCGCCGAAAAATTGTTAGACTTAGAGCCTGAAAAGGCGGAGAACTACGTGCTGCTGTCTAACTTGTATGCCGCATCAGGTAAATGGGATAGTGTGAGAAACGTGCGGCGAAAGATGAAGGAGATTGGCCTTCAGAAAGATGCCGGACGCAGCTGGATTGAACTCGGAGGGCAAGTTTACAGCTTTGTTGTTGGTGACACATCTCTGCCAGAGTCCGGAGAGATCAAGAAAACGTGGGCAAGGCTAGAGGAAAAGATAAGTGAATTCGGATACAAACCCGACACAGGCTGTGTGCTTCATGAACTGGGAGAAGACGAGAAGGTTGAAATACTACGGGGACATAGCGAGAAGCTTGCGATTTCATTCGGTTTGTTGAAGACGAGTAGAAGTAGAACAACACTGAGAGTCTGCAAAAATCTGCGGATTTGTGTGGATTGCCACAATGCAGCTAAGCTGATATCGAAAGTTGTTGAAAGGGAGATAATTGTGAGAGATAATAAGCGCTTCCATCATTTCAAACATGGCCTTTGTTCTTGCGGAGATTATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

977

Amino Acids

108.2

Weight (kDa)

6.14

Isoelectric Point (pI)

42.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	466 - 514	1.3e-08	PPR repeat family
PPR	PF01535	469 - 498	1.9e-06	PPR repeat
PPR_2	PF13041	568 - 613	3.2e-06	PPR repeat family
PPR	PF01535	569 - 597	6.2e-08	PPR repeat
PPR_2	PF13041	668 - 715	3.8e-10	PPR repeat family
PPR	PF01535	671 - 699	2.8e-06	PPR repeat
E_motif	PF20431	785 - 847	5.4e-17	E motif
DYW_deaminase	PF14432	883 - 976	7.3e-40	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18485
fragaria_vesca	FvH4_5g13240
malus_domestica	MD06G1207000.v1.1
prunus_persica	Prupe.5G211400_v2.0.a1
pyrus_communis	pycom06g18480
rosa_chinensis	RchiOBHm_Chr7g0183921
rosa_laevigata	RLG00000005010
rosa_multiflora	Rmu_sc0004712.1_g000029 Rmu_sc0007533.1_g000009
rosa_rugosa	Rorug06G0461400
rosa_samantha	Rh7AG062600 Rh7BG062800 Rh7CG064100 Rh7DG062600
rosa_wichuraiana	Rw7G005170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2024
AatII	GACGTC	2 cut(s)	183, 324
AccB1I	GGYRCC	2 cut(s)	19, 309
AccB7I	CCANNNNNTGG	1 cut(s)	349
AccBSI	CCGCTC	1 cut(s)	444
AccI	GTMKAC	1 cut(s)	362
AccIII	TCCGGA	1 cut(s)	2594
AclI	AACGTT	1 cut(s)	2019
AclWI	GGATC	4 cut(s)	837, 1439, 1490, 1503
AcoI	YGGCCR	2 cut(s)	2251, 2260
AcsI	RAATTY	8 cut(s)	778, 793, 1102, 1357, 1505, 2040, 2127, 2642
AcuI	CTGAAG	3 cut(s)	482, 2059, 2495
AcyI	GRCGYC	5 cut(s)	180, 198, 279, 310, 321
AfaI	GTAC	6 cut(s)	885, 1028, 1043, 1337, 1469, 2201
AfeI	AGCGCT	1 cut(s)	2882
AfiI	CCNNNNNNNGG	6 cut(s)	214, 349, 618, 2269, 2594, 2663
AflIII	ACRYGT	2 cut(s)	1023, 1218
AgeI	ACCGGT	1 cut(s)	1986
AhdI	GACNNNNNGTC	1 cut(s)	338
AjiI	CACGTC	1 cut(s)	631
AjnI	CCWGG	2 cut(s)	990, 1406
AjuI	GAANNNNNNNTTGG	4 cut(s)	91, 123, 998, 1030
AloI	GAACNNNNNNTCC	2 cut(s)	633, 665
Alw21I	GWGCWC	3 cut(s)	238, 2073, 2330
Alw26I	GTCTC	2 cut(s)	358, 1382
AlwI	GGATC	4 cut(s)	837, 1439, 1490, 1503
Aor13HI	TCCGGA	1 cut(s)	2594
Aor51HI	AGCGCT	1 cut(s)	2882
AoxI	GGCC	6 cut(s)	300, 609, 2251, 2260, 2506, 2902
ApeKI	GCWGC	8 cut(s)	746, 1331, 1585, 1873, 1956, 2431, 2529, 2825
ApoI	RAATTY	8 cut(s)	778, 793, 1102, 1357, 1505, 2040, 2127, 2642
ArsI	GACNNNNNNTTYG	2 cut(s)	1334, 1366
AseI	ATTAAT	1 cut(s)	998
AsiGI	ACCGGT	1 cut(s)	1986
Asp700I	GAANNNNTTC	1 cut(s)	476
AspLEI	GCGC	3 cut(s)	312, 493, 2883
AsuC2I	CCSGG	2 cut(s)	2152, 2255
AsuHPI	GGTGA	5 cut(s)	916, 1015, 1198, 1207, 2585
BaeGI	GKGCMC	2 cut(s)	1435, 1540
BamHI	GGATCC	1 cut(s)	1495
BanI	GGYRCC	2 cut(s)	19, 309
BanII	GRGCYC	3 cut(s)	1406, 2073, 2330
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BbsI	GAAGAC	4 cut(s)	1400, 1914, 2697, 2762
Bbv12I	GWGCWC	3 cut(s)	238, 2073, 2330
BbvI	GCAGC	8 cut(s)	758, 1343, 1572, 1860, 1943, 2418, 2541, 2837
BccI	CCATC	5 cut(s)	652, 664, 1168, 2102, 2895
BcgI	CGANNNNNNTGC	4 cut(s)	304, 338, 2714, 2748
BciT130I	CCWGG	2 cut(s)	992, 1408
BciVI	GTATCC	1 cut(s)	2642
BclI	TGATCA	1 cut(s)	555
BcnI	CCSGG	2 cut(s)	2152, 2255
BcoDI	GTCTC	2 cut(s)	358, 1382
BfaI	CTAG	5 cut(s)	881, 1125, 2219, 2316, 2624
BfmI	CTRYAG	1 cut(s)	2344
BfoI	RGCGCY	2 cut(s)	313, 2884
BfuI	GTATCC	1 cut(s)	2642
BglI	GCCNNNNNGGC	1 cut(s)	2259
BlpI	GCTNAGC	2 cut(s)	1365, 2829
Bme1390I	CCNGG	4 cut(s)	992, 1408, 2152, 2255
BmeRI	GACNNNNNGTC	1 cut(s)	338
BmgBI	CACGTC	1 cut(s)	631
BmiI	GGNNCC	6 cut(s)	21, 289, 311, 1405, 1497, 2307
BmrFI	CCNGG	4 cut(s)	992, 1408, 2152, 2255
BmrI	ACTGGG	1 cut(s)	2693
BmuI	ACTGGG	1 cut(s)	2693
BpiI	GAAGAC	4 cut(s)	1400, 1914, 2697, 2762
Bpu1102I	GCTNAGC	2 cut(s)	1365, 2829
BpuEI	CTTGAG	1 cut(s)	1041
BpuMI	CCSGG	2 cut(s)	2152, 2255
BsaAI	YACGTR	1 cut(s)	2428
BsaHI	GRCGYC	5 cut(s)	180, 198, 279, 310, 321
BsaI	GGTCTC	1 cut(s)	358
BsaJI	CCNNGG	1 cut(s)	282
BsaWI	WCCGGW	4 cut(s)	329, 886, 1986, 2594
BsaXI	ACNNNNNCTCC	4 cut(s)	57, 87, 2538, 2568
Bsc4I	CCNNNNNNNGG	6 cut(s)	214, 349, 618, 2269, 2594, 2663
Bse118I	RCCGGY	4 cut(s)	1225, 1727, 1986, 2262
Bse1I	ACTGG	3 cut(s)	481, 1314, 2688
Bse3DI	GCAATG	5 cut(s)	423, 706, 1645, 1725, 2330
BseAI	TCCGGA	1 cut(s)	2594
BseBI	CCWGG	2 cut(s)	992, 1408
BseDI	CCNNGG	1 cut(s)	282
BseGI	GGATG	8 cut(s)	663, 691, 748, 895, 957, 1141, 1641, 2008
BseLI	CCNNNNNNNGG	6 cut(s)	214, 349, 618, 2269, 2594, 2663
BseMI	GCAATG	5 cut(s)	423, 706, 1645, 1725, 2330
BseMII	CTCAG	7 cut(s)	204, 459, 762, 1059, 1356, 1751, 2771
BseNI	ACTGG	3 cut(s)	481, 1314, 2688
BseRI	GAGGAG	3 cut(s)	1196, 1253, 2318
BseSI	GKGCMC	2 cut(s)	1435, 1540
BseXI	GCAGC	8 cut(s)	758, 1343, 1572, 1860, 1943, 2418, 2541, 2837
BseYI	CCCAGC	1 cut(s)	926
Bsh1285I	CGRYCG	1 cut(s)	366
BshFI	GGCC	6 cut(s)	302, 611, 2253, 2262, 2508, 2904
BshNI	GGYRCC	2 cut(s)	19, 309
BshTI	ACCGGT	1 cut(s)	1986
BsiEI	CGRYCG	1 cut(s)	366
BsiHKAI	GWGCWC	3 cut(s)	238, 2073, 2330
BslFI	GGGAC	2 cut(s)	654, 2730
BslI	CCNNNNNNNGG	6 cut(s)	214, 349, 618, 2269, 2594, 2663
BsmAI	GTCTC	2 cut(s)	358, 1382
BsmBI	CGTCTC	1 cut(s)	1382
BsmFI	GGGAC	2 cut(s)	654, 2730
BsmI	GAATGC	2 cut(s)	493, 1720
BsnI	GGCC	6 cut(s)	302, 611, 2253, 2262, 2508, 2904
Bso31I	GGTCTC	1 cut(s)	358
Bsp1286I	GDGCHC	6 cut(s)	238, 1406, 1435, 1540, 2073, 2330
Bsp13I	TCCGGA	1 cut(s)	2594
Bsp1407I	TGTACA	1 cut(s)	2199
Bsp143I	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
Bsp1720I	GCTNAGC	2 cut(s)	1365, 2829
Bsp19I	CCATGG	1 cut(s)	282
BspANI	GGCC	6 cut(s)	302, 611, 2253, 2262, 2508, 2904
BspCNI	CTCAG	7 cut(s)	205, 458, 763, 1058, 1357, 1750, 2772
BspEI	TCCGGA	1 cut(s)	2594
BspHI	TCATGA	2 cut(s)	832, 2677
BspLI	GGNNCC	6 cut(s)	21, 289, 311, 1405, 1497, 2307
BspPI	GGATC	4 cut(s)	837, 1439, 1490, 1503
BspQI	GCTCTTC	1 cut(s)	2078
BspT107I	GGYRCC	2 cut(s)	19, 309
BspTNI	GGTCTC	1 cut(s)	358
BsrBI	CCGCTC	1 cut(s)	444
BsrDI	GCAATG	5 cut(s)	423, 706, 1645, 1725, 2330
BsrFI	RCCGGY	4 cut(s)	1225, 1727, 1986, 2262
BsrGI	TGTACA	1 cut(s)	2199
BsrI	ACTGG	3 cut(s)	481, 1314, 2688
BssAI	RCCGGY	4 cut(s)	1225, 1727, 1986, 2262
BssECI	CCNNGG	1 cut(s)	282
BssMI	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
BssNI	GRCGYC	5 cut(s)	180, 198, 279, 310, 321
BssT1I	CCWWGG	1 cut(s)	282
Bst2UI	CCWGG	2 cut(s)	992, 1408
Bst4CI	ACNGT	4 cut(s)	367, 910, 1399, 1848
Bst6I	CTCTTC	3 cut(s)	458, 1173, 2078
BstACI	GRCGYC	5 cut(s)	180, 198, 279, 310, 321
BstAPI	GCANNNNNTGC	1 cut(s)	1724
BstAUI	TGTACA	1 cut(s)	2199
BstBAI	YACGTR	1 cut(s)	2428
BstC8I	GCNNGC	5 cut(s)	304, 308, 1897, 2260, 2733
BstDSI	CCRYGG	1 cut(s)	282
BstEII	GGTNACC	1 cut(s)	1204
BstENI	CCTNNNNNAGG	1 cut(s)	212
BstF5I	GGATG	8 cut(s)	663, 691, 748, 895, 957, 1141, 1641, 2008
BstH2I	RGCGCY	2 cut(s)	313, 2884
BstHHI	GCGC	3 cut(s)	312, 493, 2883
BstKTI	GATC	8 cut(s)	541, 558, 832, 1156, 1447, 1498, 1768, 2604
BstMAI	GTCTC	2 cut(s)	358, 1382
BstMBI	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
BstMCI	CGRYCG	1 cut(s)	366
BstNI	CCWGG	2 cut(s)	992, 1408
BstNSI	RCATGY	5 cut(s)	1027, 1828, 2142, 2235, 2248
BstPI	GGTNACC	1 cut(s)	1204
BstSCI	CCNGG	4 cut(s)	990, 1406, 2150, 2253
BstSFI	CTRYAG	1 cut(s)	2344
BstSLI	GKGCMC	2 cut(s)	1435, 1540
BstV1I	GCAGC	8 cut(s)	758, 1343, 1572, 1860, 1943, 2418, 2541, 2837
BstV2I	GAAGAC	4 cut(s)	1400, 1914, 2697, 2762
BstX2I	RGATCY	2 cut(s)	1444, 1495
BstYI	RGATCY	2 cut(s)	1444, 1495
BsuI	GTATCC	1 cut(s)	2642
BsuRI	GGCC	6 cut(s)	302, 611, 2253, 2262, 2508, 2904
BtgI	CCRYGG	1 cut(s)	282
BtgZI	GCGATG	1 cut(s)	728
BtrI	CACGTC	1 cut(s)	631
BtsCI	GGATG	8 cut(s)	663, 691, 748, 895, 957, 1141, 1641, 2008
BtsI	GCAGTG	3 cut(s)	21, 382, 978
BtsIMutI	CAGTG	6 cut(s)	21, 382, 488, 978, 1404, 2777
Cac8I	GCNNGC	5 cut(s)	304, 308, 1897, 2260, 2733
CciI	TCATGA	2 cut(s)	832, 2677
CfoI	GCGC	3 cut(s)	312, 493, 2883
Cfr10I	RCCGGY	4 cut(s)	1225, 1727, 1986, 2262
CseI	GACGC	5 cut(s)	206, 287, 342, 348, 2535
Csp6I	GTAC	6 cut(s)	884, 1027, 1042, 1336, 1468, 2200
CspAI	ACCGGT	1 cut(s)	1986
CviQI	GTAC	6 cut(s)	884, 1027, 1042, 1336, 1468, 2200
DinI	GGCGCC	1 cut(s)	311
DpnI	GATC	8 cut(s)	540, 557, 831, 1155, 1446, 1497, 1767, 2603
DpnII	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
DraI	TTTAAA	2 cut(s)	574, 2280
DriI	GACNNNNNGTC	1 cut(s)	338
EaeI	YGGCCR	2 cut(s)	2251, 2260
Eam1104I	CTCTTC	3 cut(s)	458, 1173, 2078
Eam1105I	GACNNNNNGTC	1 cut(s)	338
EarI	CTCTTC	3 cut(s)	458, 1173, 2078
EciI	GGCGGA	4 cut(s)	65, 109, 1034, 2432
Ecl136II	GAGCTC	2 cut(s)	2071, 2328
Eco130I	CCWWGG	1 cut(s)	282
Eco147I	AGGCCT	2 cut(s)	302, 611
Eco24I	GRGCYC	3 cut(s)	1406, 2073, 2330
Eco31I	GGTCTC	1 cut(s)	358
Eco32I	GATATC	1 cut(s)	2838
Eco47III	AGCGCT	1 cut(s)	2882
Eco53kI	GAGCTC	2 cut(s)	2071, 2328
Eco57I	CTGAAG	3 cut(s)	482, 2059, 2495
Eco91I	GGTNACC	1 cut(s)	1204
EcoICRI	GAGCTC	2 cut(s)	2071, 2328
EcoNI	CCTNNNNNAGG	1 cut(s)	212
EcoO65I	GGTNACC	1 cut(s)	1204
EcoRI	GAATTC	4 cut(s)	793, 1102, 2040, 2642
EcoRII	CCWGG	2 cut(s)	990, 1406
EcoRV	GATATC	1 cut(s)	2838
EcoT14I	CCWWGG	1 cut(s)	282
EcoT22I	ATGCAT	1 cut(s)	2233
EcoT38I	GRGCYC	3 cut(s)	1406, 2073, 2330
EgeI	GGCGCC	1 cut(s)	311
EheI	GGCGCC	1 cut(s)	311
ErhI	CCWWGG	1 cut(s)	282
Esp3I	CGTCTC	1 cut(s)	1382
FaqI	GGGAC	2 cut(s)	654, 2730
FauI	CCCGC	4 cut(s)	299, 449, 722, 800
FbaI	TGATCA	1 cut(s)	555
FblI	GTMKAC	1 cut(s)	362
FokI	GGATG	8 cut(s)	670, 698, 755, 902, 964, 1148, 1628, 2015
FriOI	GRGCYC	3 cut(s)	1406, 2073, 2330
FspBI	CTAG	5 cut(s)	881, 1125, 2219, 2316, 2624
GlaI	GCGC	3 cut(s)	311, 492, 2882
GsaI	CCCAGC	1 cut(s)	930
HaeII	RGCGCY	2 cut(s)	313, 2884
HaeIII	GGCC	6 cut(s)	302, 611, 2253, 2262, 2508, 2904
HgaI	GACGC	5 cut(s)	206, 287, 342, 348, 2535
HhaI	GCGC	3 cut(s)	312, 493, 2883
Hin1I	GRCGYC	5 cut(s)	180, 198, 279, 310, 321
Hin6I	GCGC	3 cut(s)	310, 491, 2881
HinP1I	GCGC	3 cut(s)	310, 491, 2881
HincII	GTYRAC	3 cut(s)	184, 363, 1992
HindII	GTYRAC	3 cut(s)	184, 363, 1992
HindIII	AAGCTT	4 cut(s)	1455, 2194, 2274, 2729
HpaI	GTTAAC	1 cut(s)	1992
HphI	GGTGA	5 cut(s)	916, 1015, 1198, 1207, 2585
Hpy166II	GTNNAC	9 cut(s)	184, 343, 363, 1338, 1639, 1992, 2115, 2242, 2557
Hpy8I	GTNNAC	9 cut(s)	184, 343, 363, 1338, 1639, 1992, 2115, 2242, 2557
Hpy99I	CGWCG	3 cut(s)	326, 364, 458
HpyCH4III	ACNGT	4 cut(s)	367, 910, 1399, 1848
Hsp92I	GRCGYC	5 cut(s)	180, 198, 279, 310, 321
HspAI	GCGC	3 cut(s)	310, 491, 2881
KasI	GGCGCC	1 cut(s)	309
Kpn2I	TCCGGA	1 cut(s)	2594
Ksp22I	TGATCA	1 cut(s)	555
KspAI	GTTAAC	1 cut(s)	1992
Kzo9I	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
LguI	GCTCTTC	1 cut(s)	2078
LmnI	GCTCC	3 cut(s)	1409, 2305, 2325
Lsp1109I	GCAGC	8 cut(s)	758, 1343, 1572, 1860, 1943, 2418, 2541, 2837
MaeI	CTAG	5 cut(s)	881, 1125, 2219, 2316, 2624
MalI	GATC	8 cut(s)	540, 557, 831, 1155, 1446, 1497, 1767, 2603
MbiI	CCGCTC	1 cut(s)	444
MboI	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
MflI	RGATCY	2 cut(s)	1444, 1495
MhlI	GDGCHC	6 cut(s)	238, 1406, 1435, 1540, 2073, 2330
Mly113I	GGCGCC	1 cut(s)	310
MlyI	GAGTC	5 cut(s)	152, 1250, 2359, 2600, 2793
MmeI	TCCRAC	3 cut(s)	1224, 1723, 2247
Mph1103I	ATGCAT	1 cut(s)	2233
MroI	TCCGGA	1 cut(s)	2594
MroXI	GAANNNNTTC	1 cut(s)	476
MseI	TTAA	7 cut(s)	573, 606, 998, 1991, 2132, 2160, 2279
MslI	CAYNNNNRTG	4 cut(s)	34, 407, 1937, 2049
MspA1I	CMGCKG	2 cut(s)	1364, 2532
MspR9I	CCNGG	4 cut(s)	992, 1408, 2152, 2255
MssI	GTTTAAAC	1 cut(s)	574
Mva1269I	GAATGC	2 cut(s)	493, 1720
MvaI	CCWGG	2 cut(s)	992, 1408
NarI	GGCGCC	1 cut(s)	310
NciI	CCSGG	2 cut(s)	2152, 2255
NcoI	CCATGG	1 cut(s)	282
NdeII	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
NlaIV	GGNNCC	6 cut(s)	21, 289, 311, 1405, 1497, 2307
NmuCI	GTSAC	6 cut(s)	194, 377, 904, 1204, 1350, 2573
NsiI	ATGCAT	1 cut(s)	2233
NspI	RCATGY	5 cut(s)	1027, 1828, 2142, 2235, 2248
PagI	TCATGA	2 cut(s)	832, 2677
PceI	AGGCCT	2 cut(s)	302, 611
PciI	ACATGT	1 cut(s)	1023
PciSI	GCTCTTC	1 cut(s)	2078
PctI	GAATGC	2 cut(s)	493, 1720
PdmI	GAANNNNTTC	1 cut(s)	476
PfeI	GAWTC	5 cut(s)	437, 958, 1570, 1607, 1691
PflMI	CCANNNNNTGG	1 cut(s)	349
PfoI	TCCNGGA	1 cut(s)	1406
PinAI	ACCGGT	1 cut(s)	1986
PleI	GAGTC	5 cut(s)	152, 1249, 2358, 2599, 2792
PluTI	GGCGCC	1 cut(s)	313
PmeI	GTTTAAAC	1 cut(s)	574
PpsI	GAGTC	5 cut(s)	152, 1249, 2358, 2599, 2792
Ppu21I	YACGTR	1 cut(s)	2428
PscI	ACATGT	1 cut(s)	1023
PshBI	ATTAAT	1 cut(s)	998
PsiI	TTATAA	1 cut(s)	2024
Psp124BI	GAGCTC	2 cut(s)	2073, 2330
Psp1406I	AACGTT	1 cut(s)	2019
Psp6I	CCWGG	2 cut(s)	990, 1406
PspEI	GGTNACC	1 cut(s)	1204
PspFI	CCCAGC	1 cut(s)	926
PspGI	CCWGG	2 cut(s)	990, 1406
PspN4I	GGNNCC	6 cut(s)	21, 289, 311, 1405, 1497, 2307
PsuI	RGATCY	2 cut(s)	1444, 1495
PvuII	CAGCTG	1 cut(s)	2532
RsaI	GTAC	6 cut(s)	885, 1028, 1043, 1337, 1469, 2201
RsaNI	GTAC	6 cut(s)	884, 1027, 1042, 1336, 1468, 2200
RseI	CAYNNNNRTG	4 cut(s)	34, 407, 1937, 2049
SacI	GAGCTC	2 cut(s)	2073, 2330
SalI	GTCGAC	1 cut(s)	361
SapI	GCTCTTC	1 cut(s)	2078
SaqAI	TTAA	7 cut(s)	573, 606, 998, 1991, 2132, 2160, 2279
Sau3AI	GATC	8 cut(s)	538, 555, 829, 1153, 1444, 1495, 1765, 2601
SchI	GAGTC	5 cut(s)	152, 1250, 2359, 2600, 2793
ScrFI	CCNGG	4 cut(s)	992, 1408, 2152, 2255
SduI	GDGCHC	6 cut(s)	238, 1406, 1435, 1540, 2073, 2330
SfcI	CTRYAG	1 cut(s)	2344
SfiI	GGCCNNNNNGGCC	1 cut(s)	2259
SfoI	GGCGCC	1 cut(s)	311
SmiMI	CAYNNNNRTG	4 cut(s)	34, 407, 1937, 2049
SmlI	CTYRAG	1 cut(s)	1056
SmoI	CTYRAG	1 cut(s)	1056
SseBI	AGGCCT	2 cut(s)	302, 611
SspDI	GGCGCC	1 cut(s)	309
SspI	AATATT	3 cut(s)	1484, 1674, 2177
SspMI	CTAG	5 cut(s)	881, 1125, 2219, 2316, 2624
SstI	GAGCTC	2 cut(s)	2073, 2330
StuI	AGGCCT	2 cut(s)	302, 611
StyD4I	CCNGG	4 cut(s)	990, 1406, 2150, 2253
StyI	CCWWGG	1 cut(s)	282
TaaI	ACNGT	4 cut(s)	367, 910, 1399, 1848
TaqI	TCGA	8 cut(s)	232, 324, 362, 453, 759, 797, 1962, 2840
TatI	WGTACW	2 cut(s)	1026, 2199
TauI	GCSGC	4 cut(s)	125, 315, 2453, 2488
TfiI	GAWTC	5 cut(s)	437, 958, 1570, 1607, 1691
Tru1I	TTAA	7 cut(s)	573, 606, 998, 1991, 2132, 2160, 2279
Tru9I	TTAA	7 cut(s)	573, 606, 998, 1991, 2132, 2160, 2279
TscAI	CASTG	6 cut(s)	21, 382, 488, 978, 1404, 2784
TseFI	GTSAC	6 cut(s)	194, 377, 904, 1204, 1350, 2573
TseI	GCWGC	8 cut(s)	746, 1331, 1585, 1873, 1956, 2431, 2529, 2825
Tsp45I	GTSAC	6 cut(s)	194, 377, 904, 1204, 1350, 2573
TspGWI	ACGGA	4 cut(s)	581, 948, 1096, 1624
TspRI	CASTG	6 cut(s)	21, 382, 488, 978, 1404, 2784
Van91I	CCANNNNNTGG	1 cut(s)	349
VspI	ATTAAT	1 cut(s)	998
XagI	CCTNNNNNAGG	1 cut(s)	212
XapI	RAATTY	8 cut(s)	778, 793, 1102, 1357, 1505, 2040, 2127, 2642
XbaI	TCTAGA	1 cut(s)	1124
XceI	RCATGY	5 cut(s)	1027, 1828, 2142, 2235, 2248
XmiI	GTMKAC	1 cut(s)	362
XmnI	GAANNNNTTC	1 cut(s)	476
XspI	CTAG	5 cut(s)	881, 1125, 2219, 2316, 2624
ZraI	GACGTC	2 cut(s)	181, 322
Zsp2I	ATGCAT	1 cut(s)	2233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.