AT1G18660

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009065
Physical Location & Seq
Forward (+)
6420856 .. 6425898
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18660.4

Sequence Viewer

Length: 1461 bp
ATGTCTAACGAAGACTCTCTTCCGCCTTTCACTTTGTTTGGTTTGGATGATGTCGAAAATTACGGTCTGGTCACTGAAGCAGACAATTCGTTACCTTTAGATATACACAATCAGGTTTTTCAACTTGTGGAGAAGGGAAACCAATCATTCAAAGAGTCTCGCTTTGAAGAGGCTATTAGCAGTTATTCAAAGGCCAATTCTATTAAACCTCTTGACCCTATTGTTCTTGGCAATAGAAGTGCTGCTTATATAAGATTTGGCCAATATCTGAAGCAGAGATCTGCATCAATTTCTGAATATAAACCTCTGAATGGTTTTGATATGTCGATGCTTGGTGAACTTGCTCTTAAGGATGCTGATAAGCTAATGAGTCTTCAGAGCAGTTTAGTGAAGTCATATATTACAAAGGCTTGTGCCCTCATGTTGCTAGAAAGATATGAGGTAGCCCGTGACACTATCCTCTCAGGTCTACAGATTGATCCCTTTAGCGGTCCTCTACGATCCAATCTTCAGGAATTGGAGAAAGTGATGCCTAATTCGATGCGTAAAACCCATGGAATGGCTGAGCGGTCTGATGATTTTGATTGCACTGTTTGCCTGAAATTGCTGTACGAACCTGCTACAACTCCATGTGGGCATACATTCTGCCGATCATGCCTCTTTCAGTCAATGGATCGCGGCAACAAATGTCCACTATGTCGAACTGTCATTTTTATGACTCCAAGAACATGCGCTGTCAGTGTGACACTGAATAACATCATAGAGAAAAACTTTCCAGAGGAGTATGCTGAAAGGAAATCAGAGCAAGACACTCTGGTCCACCTAGGCAATGAAAGTATGCCCCTTTTCGTCATGGATGTGATAATCCCCTGTCAGAAGTTGTCTCTTCATATATTTGAACCACGATATCGACTAATGGTGAGAAGAATAATGGAAGGAAATCATCGGATGGGAATGGTAGCTCTTGATTCGGCGACAGGTTCTCCAGTGGATGTTGCTTGCGAAGTTGAAATCACAGAGTGTGATCCACTCCCGGATGGACGTTTTGTCCTGGAGCTGGAAAGCCATAGAAGGTGCCGCATTGTAAAAGCTTGGGATCAAGATGGATATCGTGTTGCAGAGGTTGAATGGGTGAAAGATATACCACCACAAAGCGAGCAAGGGAAAGCAGATCTGCGGGAACTGACGACCAGCGCAGCATCATTTGCTCGGTCATGGCTAGATAGAGCAAAGGAAGCAGCTAGACAAGGAGACAGAAGAAGACTTGAAATACTTCTAAATGTTGAATCTATGATACCAACGCCTCAAGATCCCGAGCGCTTCAGTTTCTGGCTTGCTACACTGACAGATAGGAGACCTTCAGAAAGATTGGAACTACTTCGGCTTCAGGATACTGGAGAGAGAATAAAGCGCGGGTTGATATATCTCCGATCAGTAGAAAGAGGATGCAGAATGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg zf-C3HC4_2

Protein Analysis

486

Amino Acids

55.41

Weight (kDa)

5.73

Isoelectric Point (pI)

54.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 194 - 237 1.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 195 - 234 7.4e-09 Ring finger domain
zf-C3HC4_2 PF13923 196 - 233 7.3e-13 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 196 - 233 9.8e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 196 - 231 3.2e-08 RING-type zinc-finger
zf-C3HC4_4 PF15227 196 - 233 5.1e-07 zinc finger of C3HC4-type, RING
zf-RING_5 PF14634 196 - 235 3.2e-06 zinc-RING finger domain
LON_substr_bdg PF02190 280 - 465 2.4e-21 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 815
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 1074
AccB7I CCANNNNNTGG 1 cut(s) 559
AccBSI CCGCTC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 469
AccII CGCG 2 cut(s) 678, 1413
AciI CCGC 7 cut(s) 23, 489, 568, 678, 1078, 1177, 1413
AclWI GGATC 6 cut(s) 473, 495, 681, 1019, 1104, 1304
AcoI YGGCCR 1 cut(s) 259
AcuI CTGAAG 7 cut(s) 96, 290, 359, 494, 1306, 1344, 1370
AdeI CACNNNGTG 1 cut(s) 1020
AfaI GTAC 1 cut(s) 611
AfeI AGCGCT 1 cut(s) 1319
AfiI CCNNNNNNNGG 4 cut(s) 311, 488, 559, 1057
AflII CTTAAG 1 cut(s) 347
AgsI TTSAA 9 cut(s) 122, 151, 167, 189, 899, 1010, 1127, 1268, 1286
AhdI GACNNNNNGTC 1 cut(s) 1046
AjnI CCWGG 1 cut(s) 1050
AluBI AGCT 5 cut(s) 364, 962, 1057, 1091, 1241
AluI AGCT 5 cut(s) 364, 962, 1057, 1091, 1241
Alw26I GTCTC 4 cut(s) 162, 888, 1245, 1348
AlwI GGATC 6 cut(s) 473, 495, 681, 1019, 1104, 1304
AlwNI CAGNNNCTG 1 cut(s) 1329
Ama87I CYCGRG 1 cut(s) 1313
Aor51HI AGCGCT 1 cut(s) 1319
AoxI GGCC 2 cut(s) 192, 259
ApeKI GCWGC 3 cut(s) 242, 1196, 1238
Asp700I GAANNNNTTC 2 cut(s) 1272, 1377
AspA2I CCTAGG 1 cut(s) 823
AspLEI GCGC 4 cut(s) 734, 1196, 1320, 1413
AspS9I GGNCC 2 cut(s) 491, 817
AsuC2I CCSGG 1 cut(s) 1034
AsuHPI GGTGA 3 cut(s) 347, 931, 1144
AvaI CYCGRG 1 cut(s) 1313
AvaII GGWCC 2 cut(s) 491, 817
AvrII CCTAGG 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 418
BalI TGGCCA 1 cut(s) 261
BanI GGYRCC 1 cut(s) 1074
BbsI GAAGAC 3 cut(s) 18, 365, 1267
BbvI GCAGC 3 cut(s) 229, 1208, 1250
BccI CCATC 3 cut(s) 943, 1031, 1097
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciT130I CCWGG 1 cut(s) 1052
BciVI GTATCC 1 cut(s) 1384
BcnI CCSGG 1 cut(s) 1034
BcoDI GTCTC 4 cut(s) 162, 888, 1245, 1348
BfaI CTAG 4 cut(s) 428, 824, 1220, 1242
BfmI CTRYAG 1 cut(s) 470
BfoI RGCGCY 1 cut(s) 1321
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 625
BfuI GTATCC 1 cut(s) 1384
BglII AGATCT 2 cut(s) 278, 1171
BisI GCNGC 5 cut(s) 243, 679, 1078, 1197, 1239
BlnI CCTAGG 1 cut(s) 823
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 5 cut(s) 244, 680, 1079, 1198, 1240
Bme1390I CCNGG 2 cut(s) 1034, 1052
Bme18I GGWCC 2 cut(s) 491, 817
BmeRI GACNNNNNGTC 1 cut(s) 1046
BmeT110I CYCGRG 1 cut(s) 1313
BmgT120I GGNCC 2 cut(s) 491, 817
BmiI GGNNCC 1 cut(s) 1076
BmrFI CCNGG 2 cut(s) 1034, 1052
BmsI GCATC 7 cut(s) 293, 318, 343, 519, 531, 1208, 1436
BpiI GAAGAC 3 cut(s) 18, 365, 1267
BpmI CTGGAG 3 cut(s) 969, 1073, 1416
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 1290
BpuMI CCSGG 1 cut(s) 1034
BsaBI GATNNNNATC 2 cut(s) 283, 1107
BsaI GGTCTC 1 cut(s) 1348
BsaJI CCNNGG 2 cut(s) 553, 823
BsaXI ACNNNNNCTCC 2 cut(s) 967, 997
Bsc4I CCNNNNNNNGG 4 cut(s) 311, 488, 559, 1057
Bse1I ACTGG 2 cut(s) 986, 1399
Bse3DI GCAATG 1 cut(s) 835
Bse8I GATNNNNATC 2 cut(s) 283, 1107
BseBI CCWGG 1 cut(s) 1052
BseDI CCNNGG 2 cut(s) 553, 823
BseGI GGATG 7 cut(s) 52, 358, 862, 954, 997, 1042, 1451
BseJI GATNNNNATC 2 cut(s) 283, 1107
BseLI CCNNNNNNNGG 4 cut(s) 311, 488, 559, 1057
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 2 cut(s) 477, 555
BseNI ACTGG 2 cut(s) 986, 1399
BseRI GAGGAG 1 cut(s) 794
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 3 cut(s) 229, 1208, 1250
Bsh1236I CGCG 2 cut(s) 678, 1413
BshFI GGCC 2 cut(s) 194, 261
BshNI GGYRCC 1 cut(s) 1074
BsiHKCI CYCGRG 1 cut(s) 1313
BsiSI CCGG 1 cut(s) 1034
BslI CCNNNNNNNGG 4 cut(s) 311, 488, 559, 1057
BsmAI GTCTC 4 cut(s) 162, 888, 1245, 1348
BsmI GAATGC 1 cut(s) 1458
BsnI GGCC 2 cut(s) 194, 261
Bso31I GGTCTC 1 cut(s) 1348
BsoBI CYCGRG 1 cut(s) 1313
Bsp1286I GDGCHC 1 cut(s) 418
Bsp1720I GCTNAGC 1 cut(s) 564
Bsp19I CCATGG 1 cut(s) 553
BspACI CCGC 7 cut(s) 23, 489, 568, 678, 1078, 1177, 1413
BspANI GGCC 2 cut(s) 194, 261
BspCNI CTCAG 2 cut(s) 476, 556
BspFNI CGCG 2 cut(s) 678, 1413
BspLI GGNNCC 1 cut(s) 1076
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 6 cut(s) 473, 495, 681, 1019, 1104, 1304
BspT107I GGYRCC 1 cut(s) 1074
BspTI CTTAAG 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 1348
BsrBI CCGCTC 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 835
BsrI ACTGG 2 cut(s) 986, 1399
BssECI CCNNGG 2 cut(s) 553, 823
BssT1I CCWWGG 2 cut(s) 553, 823
Bst2UI CCWGG 1 cut(s) 1052
Bst4CI ACNGT 3 cut(s) 65, 592, 706
Bst6I CTCTTC 3 cut(s) 24, 162, 891
BstAFI CTTAAG 1 cut(s) 347
BstAPI GCANNNNNTGC 2 cut(s) 594, 1205
BstC8I GCNNGC 3 cut(s) 1000, 1157, 1335
BstDEI CTNAG 2 cut(s) 463, 564
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 7 cut(s) 52, 358, 862, 954, 997, 1042, 1451
BstFNI CGCG 2 cut(s) 678, 1413
BstH2I RGCGCY 1 cut(s) 1321
BstHHI GCGC 4 cut(s) 734, 1196, 1320, 1413
BstMAI GTCTC 4 cut(s) 162, 888, 1245, 1348
BstMWI GCNNNNNNNGC 4 cut(s) 594, 654, 1205, 1235
BstNI CCWGG 1 cut(s) 1052
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 2 cut(s) 1032, 1050
BstSFI CTRYAG 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 2 cut(s) 678, 1413
BstV1I GCAGC 3 cut(s) 229, 1208, 1250
BstV2I GAAGAC 3 cut(s) 18, 365, 1267
BstX2I RGATCY 3 cut(s) 278, 1171, 1309
BstYI RGATCY 3 cut(s) 278, 1171, 1309
BsuI GTATCC 1 cut(s) 1384
BsuRI GGCC 2 cut(s) 194, 261
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 7 cut(s) 52, 358, 862, 954, 997, 1042, 1451
BtsIMutI CAGTG 6 cut(s) 72, 588, 745, 746, 993, 1340
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 3 cut(s) 1000, 1157, 1335
CaiI CAGNNNCTG 1 cut(s) 1329
CfoI GCGC 4 cut(s) 734, 1196, 1320, 1413
Cfr13I GGNCC 2 cut(s) 491, 817
Csp6I GTAC 1 cut(s) 610
CviAII CATG 7 cut(s) 421, 554, 630, 654, 729, 853, 1215
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 2 cut(s) 463, 564
DraIII CACNNNGTG 1 cut(s) 1020
DrdI GACNNNNNNGTC 1 cut(s) 815
DriI GACNNNNNGTC 1 cut(s) 1046
DseDI GACNNNNNNGTC 1 cut(s) 815
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 3 cut(s) 24, 162, 891
Eam1105I GACNNNNNGTC 1 cut(s) 1046
EarI CTCTTC 3 cut(s) 24, 162, 891
EciI GGCGGA 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 553, 823
Eco31I GGTCTC 1 cut(s) 1348
Eco32I GATATC 2 cut(s) 908, 1109
Eco47I GGWCC 2 cut(s) 491, 817
Eco47III AGCGCT 1 cut(s) 1319
Eco57I CTGAAG 7 cut(s) 96, 290, 359, 494, 1306, 1344, 1370
Eco88I CYCGRG 1 cut(s) 1313
EcoRII CCWGG 1 cut(s) 1050
EcoRV GATATC 2 cut(s) 908, 1109
EcoT14I CCWWGG 2 cut(s) 553, 823
ErhI CCWWGG 2 cut(s) 553, 823
FaeI CATG 7 cut(s) 424, 557, 633, 657, 732, 856, 1218
FalI AAGNNNNNCTT 3 cut(s) 35, 229, 261
FatI CATG 7 cut(s) 420, 553, 629, 653, 728, 852, 1214
FauI CCCGC 2 cut(s) 1170, 1406
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 5 cut(s) 243, 679, 1078, 1197, 1239
FokI GGATG 6 cut(s) 59, 365, 869, 961, 1004, 1049
Fsp4HI GCNGC 5 cut(s) 243, 679, 1078, 1197, 1239
FspBI CTAG 4 cut(s) 428, 824, 1220, 1242
GlaI GCGC 4 cut(s) 733, 1195, 1319, 1412
GluI GCNGC 5 cut(s) 243, 679, 1078, 1197, 1239
GsuI CTGGAG 3 cut(s) 969, 1073, 1416
HaeII RGCGCY 1 cut(s) 1321
HaeIII GGCC 2 cut(s) 194, 261
HapII CCGG 1 cut(s) 1034
HhaI GCGC 4 cut(s) 734, 1196, 1320, 1413
Hin1II CATG 7 cut(s) 424, 557, 633, 657, 732, 856, 1218
Hin6I GCGC 4 cut(s) 732, 1194, 1318, 1411
HinP1I GCGC 4 cut(s) 732, 1194, 1318, 1411
HindIII AAGCTT 1 cut(s) 1089
HinfI GANTC 6 cut(s) 14, 155, 370, 718, 968, 1286
HpaII CCGG 1 cut(s) 1034
HphI GGTGA 3 cut(s) 347, 931, 1144
Hpy166II GTNNAC 4 cut(s) 338, 470, 692, 820
Hpy188III TCNNGA 8 cut(s) 212, 512, 776, 965, 1100, 1307, 1313, 1388
Hpy8I GTNNAC 4 cut(s) 338, 470, 692, 820
HpyAV CCTTC 4 cut(s) 127, 929, 1065, 1368
HpyCH4III ACNGT 3 cut(s) 65, 592, 706
HpyCH4IV ACGT 1 cut(s) 1042
HpyCH4V TGCA 5 cut(s) 284, 588, 1118, 1449, 1456
HpyF10VI GCNNNNNNNGC 4 cut(s) 594, 654, 1205, 1235
HpyF3I CTNAG 2 cut(s) 463, 564
HpySE526I ACGT 1 cut(s) 1042
Hsp92II CATG 7 cut(s) 424, 557, 633, 657, 732, 856, 1218
HspAI GCGC 4 cut(s) 732, 1194, 1318, 1411
LmnI GCTCC 1 cut(s) 1054
Lsp1109I GCAGC 3 cut(s) 229, 1208, 1250
LweI GCATC 7 cut(s) 293, 318, 343, 519, 531, 1208, 1436
MaeI CTAG 4 cut(s) 428, 824, 1220, 1242
MaeII ACGT 1 cut(s) 1042
MaeIII GTNAC 4 cut(s) 70, 90, 449, 742
MbiI CCGCTC 1 cut(s) 568
MboII GAAGA 9 cut(s) 11, 23, 179, 365, 500, 878, 936, 1269, 1272
MflI RGATCY 3 cut(s) 278, 1171, 1309
MhlI GDGCHC 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 7 cut(s) 58, 85, 196, 288, 515, 535, 602
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 4 cut(s) 8, 164, 379, 712
Mox20I TGGCCA 1 cut(s) 261
MroXI GAANNNNTTC 2 cut(s) 1272, 1377
MscI TGGCCA 1 cut(s) 261
MseI TTAA 2 cut(s) 204, 348
MslI CAYNNNNRTG 2 cut(s) 713, 857
Msp20I TGGCCA 1 cut(s) 261
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 1 cut(s) 1034
MspR9I CCNGG 2 cut(s) 1034, 1052
Mva1269I GAATGC 1 cut(s) 1458
MvaI CCWGG 1 cut(s) 1052
MvnI CGCG 2 cut(s) 678, 1413
MwoI GCNNNNNNNGC 4 cut(s) 594, 654, 1205, 1235
NciI CCSGG 1 cut(s) 1034
NcoI CCATGG 1 cut(s) 553
NlaIII CATG 7 cut(s) 424, 557, 633, 657, 732, 856, 1218
NlaIV GGNNCC 1 cut(s) 1076
NmuCI GTSAC 3 cut(s) 70, 449, 742
NspI RCATGY 1 cut(s) 732
PctI GAATGC 1 cut(s) 1458
PdmI GAANNNNTTC 2 cut(s) 1272, 1377
PfeI GAWTC 2 cut(s) 968, 1286
PflMI CCANNNNNTGG 1 cut(s) 559
PfoI TCCNGGA 2 cut(s) 1032, 1050
PkrI GCNGC 5 cut(s) 244, 680, 1079, 1198, 1240
PleI GAGTC 4 cut(s) 8, 163, 378, 712
PpsI GAGTC 4 cut(s) 8, 163, 378, 712
Psp6I CCWGG 1 cut(s) 1050
PspGI CCWGG 1 cut(s) 1050
PspN4I GGNNCC 1 cut(s) 1076
PspPI GGNCC 2 cut(s) 491, 817
PstNI CAGNNNCTG 1 cut(s) 1329
PsuI RGATCY 3 cut(s) 278, 1171, 1309
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
RseI CAYNNNNRTG 2 cut(s) 713, 857
SaqAI TTAA 2 cut(s) 204, 348
SatI GCNGC 5 cut(s) 243, 679, 1078, 1197, 1239
Sau96I GGNCC 2 cut(s) 491, 817
SchI GAGTC 4 cut(s) 8, 164, 379, 712
ScrFI CCNGG 2 cut(s) 1034, 1052
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 7 cut(s) 293, 318, 343, 519, 531, 1208, 1436
SfcI CTRYAG 1 cut(s) 470
SinI GGWCC 2 cut(s) 491, 817
SmiMI CAYNNNNRTG 2 cut(s) 713, 857
SmlI CTYRAG 2 cut(s) 347, 1305
SmoI CTYRAG 2 cut(s) 347, 1305
Sse9I AATT 7 cut(s) 58, 85, 196, 288, 515, 535, 602
SsiI CCGC 7 cut(s) 23, 489, 568, 678, 1078, 1177, 1413
SspMI CTAG 4 cut(s) 428, 824, 1220, 1242
StyD4I CCNGG 2 cut(s) 1032, 1050
StyI CCWWGG 2 cut(s) 553, 823
TaaI ACNGT 3 cut(s) 65, 592, 706
TaiI ACGT 1 cut(s) 1045
TaqI TCGA 5 cut(s) 54, 326, 539, 700, 910
TaqII GACCGA 1 cut(s) 1200
TasI AATT 7 cut(s) 58, 85, 196, 288, 515, 535, 602
TauI GCSGC 2 cut(s) 681, 1080
TfiI GAWTC 2 cut(s) 968, 1286
Tru1I TTAA 2 cut(s) 204, 348
Tru9I TTAA 2 cut(s) 204, 348
TscAI CASTG 6 cut(s) 79, 595, 745, 753, 993, 1347
TseFI GTSAC 3 cut(s) 70, 449, 742
TseI GCWGC 3 cut(s) 242, 1196, 1238
Tsp45I GTSAC 3 cut(s) 70, 449, 742
TspDTI ATGAA 2 cut(s) 846, 878
TspRI CASTG 6 cut(s) 79, 595, 745, 753, 993, 1347
Van91I CCANNNNNTGG 1 cut(s) 559
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 491, 817
XceI RCATGY 1 cut(s) 732
XmaJI CCTAGG 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 469
XmnI GAANNNNTTC 2 cut(s) 1272, 1377
XspI CTAG 4 cut(s) 428, 824, 1220, 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.