MD06G1197800.v1.1

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009065
Physical Location & Seq
Reverse (-)
33154855 .. 33160783
5929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1197800.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGTCCAGCGAGGCCGAGCCGTCGCCGTCCGGGTTCGGCACCGACGTGCCTAGCGACGTCGAGGACTACATTTCGGCGAATGCAGGAGAGCCCTCATTGCCCTGGGACATATTTGGTCACGTTTTTGACATTGTGAAGAATGGAAATCAGGCATTTCGAGAGAATCGCTTTGAGGAGGCAATTAATTGTTACTCCAGAGCCAATAACATTAAACCATGTGATCCAATTATTCTTGCCAACCGATGTGCTGCTTATATCAGGATCAGTAAATTCCTTAAACGCTTACTGGCATCAGCTTCTGAATATAGAGCACGGACTGGATTGGATCCCACAACACTTGCTGAACTTGCGTTAAAGGATGCAGAGAAGCTAATCGACCTTCGAAGTAATACAGAAAAGGCGTACATATTAAAGGCCGAAGCTCTCATTTTGCTGGAGAAATATGAAATGGCCCGTGATGTTATTCTTTCGGGTCTTCAGGTTAATCCTTTTAGCAATTATCTTCAGGAATCTTTCCAGAATTTAGAGAGAATACAATCTAATTTCACAGGGATGAGAAGCCATAGAAAAGCAGAACGCAGTGATGACTTTGATTGCACACTATGCTTAAAATTACTATACAAACCTGTCACAACTCCTTGTGGGCATTCCTTTTGTCGTTCATGCCTATTTCAGTCAATGGATCGAGGTAACAAATGTCCTTTGTGCCGCACAGTTCTTTTCATTAGTCCTAGAACGTGTGCAATCAGTGTTACACTCAATGACATTATACAAAAGAACTTCCCAGAAGAATTTGCCGAAAGGAAGTTAGAAAATGACCAAATGACAAACTTTGGTGTTGATTTGATGCCACTCTTTGTAATGGATGTTGTCCTCCCATTCCAGAAGTTTCCGCTGCACATTTTTGAACCACGGTATAGACTTATGGTAAGGAGGATAATGGAAGGCAATCGTCGGATGGGAATGGTTATCATTGATTCATCAACAGGTTCAATAGCTGAATTTGCTTGCGAAGTGGAAATTACCGAGTGTGAGCCACTTCCGGATGGTCGTTTTTATTTAGAGATTGAAAGTCGGCGAAGGTTTCGCATAATTCGATCTCGGGACCAAGATGGGTACCGTGTTGCAGAGATTGAATGGGTACAAGACACTCCACCAGAAGGGTGTGAAGCGAGAGAAGAATTGCAGGAATTGGCAAGTAATGCAGCAGAGTATGCTCGATCATGGATAGCGCGGGGTAAAAGGGCAGCACGTCAAGATCGAAGACGACTTGAGAGACTTCTCGGTGTGGAAGCAATGATGCCTTCACTACAAGATCCTGAACGCTTCAGCTTTTGGCTTGCTTCCTTATCAAACCGGCCTCCTCATGAAAGGTTGGATCTTCTAAGCTTGCAAGATACAAAAGAGAGAATACACCGGGGACTGTTACATCTTCGAGCGGAAGAGCGGGGTTGTCAAGTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg zf-C3HC4_2

Protein Analysis

489

Amino Acids

56.15

Weight (kDa)

5.98

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 197 - 238 1.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 198 - 237 2e-08 Ring finger domain
zf-C3HC4_2 PF13923 199 - 236 2e-12 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 199 - 236 9.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 199 - 234 2e-08 RING-type zinc-finger
zf-C3HC4_4 PF15227 199 - 236 2.7e-07 zinc finger of C3HC4-type, RING
zf-RING_5 PF14634 199 - 238 3.7e-06 zinc-RING finger domain
LON_substr_bdg PF02190 283 - 478 1.6e-27 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
Acc65I GGTACC 1 cut(s) 1116
AccB1I GGYRCC 2 cut(s) 38, 1116
AccBSI CCGCTC 2 cut(s) 1439, 1447
AccII CGCG 1 cut(s) 1234
AccIII TCCGGA 1 cut(s) 1042
AciI CCGC 5 cut(s) 709, 893, 1234, 1439, 1447
AclWI GGATC 7 cut(s) 215, 269, 320, 333, 690, 1310, 1386
AcsI RAATTY 4 cut(s) 269, 520, 791, 1001
AcuI CTGAAG 3 cut(s) 461, 488, 1312
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 3 cut(s) 404, 1118, 1143
AfiI CCNNNNNNNGG 1 cut(s) 1160
AflIII ACRYGT 1 cut(s) 737
AgsI TTSAA 4 cut(s) 908, 993, 1070, 1136
AjiI CACGTC 2 cut(s) 46, 1253
AjnI CCWGG 1 cut(s) 101
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 6 cut(s) 296, 370, 422, 998, 1332, 1389
AluI AGCT 6 cut(s) 296, 370, 422, 998, 1332, 1389
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 1270
AlwI GGATC 7 cut(s) 215, 269, 320, 333, 690, 1310, 1386
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 1101
Aor13HI TCCGGA 1 cut(s) 1042
AoxI GGCC 4 cut(s) 12, 414, 450, 1358
ApeKI GCWGC 4 cut(s) 248, 895, 1205, 1247
ApoI RAATTY 4 cut(s) 269, 520, 791, 1001
AseI ATTAAT 1 cut(s) 183
Asp718I GGTACC 1 cut(s) 1116
AspLEI GCGC 1 cut(s) 1234
AspS9I GGNCC 2 cut(s) 451, 1105
AsuC2I CCSGG 2 cut(s) 31, 1418
AsuII TTCGAA 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 1 cut(s) 1105
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 2 cut(s) 38, 1116
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 467, 1270
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 235, 882, 1217, 1259
BccI CCATC 3 cut(s) 952, 1040, 1106
BceAI ACGGC 2 cut(s) 4, 10
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 2 cut(s) 31, 1418
BcoDI GTCTC 1 cut(s) 1270
BfaI CTAG 2 cut(s) 51, 732
BisI GCNGC 5 cut(s) 249, 709, 896, 1206, 1248
BlsI GCNGC 5 cut(s) 250, 710, 897, 1207, 1249
Bme1390I CCNGG 3 cut(s) 31, 103, 1418
Bme18I GGWCC 1 cut(s) 1105
BmeT110I CYCGRG 1 cut(s) 1101
BmgBI CACGTC 2 cut(s) 46, 1253
BmgT120I GGNCC 2 cut(s) 451, 1105
BmiI GGNNCC 4 cut(s) 40, 327, 1106, 1118
BmrFI CCNGG 3 cut(s) 31, 103, 1418
BmsI GCATC 4 cut(s) 299, 349, 837, 1290
BpiI GAAGAC 2 cut(s) 467, 1270
BpmI CTGGAG 2 cut(s) 178, 455
Bpu14I TTCGAA 1 cut(s) 382
BpuEI CTTGAG 1 cut(s) 1292
BpuMI CCSGG 2 cut(s) 31, 1418
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 4 cut(s) 101, 102, 911, 1417
BsaWI WCCGGW 1 cut(s) 1042
Bsc4I CCNNNNNNNGG 1 cut(s) 1160
Bse118I RCCGGY 1 cut(s) 1356
Bse1I ACTGG 2 cut(s) 291, 322
Bse3DI GCAATG 2 cut(s) 95, 1302
BseAI TCCGGA 1 cut(s) 1042
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 4 cut(s) 101, 102, 911, 1417
BseGI GGATG 5 cut(s) 364, 558, 871, 963, 1051
BseLI CCNNNNNNNGG 1 cut(s) 1160
BseMI GCAATG 2 cut(s) 95, 1302
BseNI ACTGG 2 cut(s) 291, 322
BseRI GAGGAG 2 cut(s) 188, 1353
BseXI GCAGC 4 cut(s) 235, 882, 1217, 1259
BsgI GTGCAG 1 cut(s) 881
Bsh1236I CGCG 1 cut(s) 1234
BshFI GGCC 4 cut(s) 14, 416, 452, 1360
BshNI GGYRCC 2 cut(s) 38, 1116
BsiHKAI GWGCWC 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 4 cut(s) 30, 1043, 1357, 1417
BslFI GGGAC 3 cut(s) 119, 1118, 1434
BslI CCNNNNNNNGG 1 cut(s) 1160
BsmAI GTCTC 1 cut(s) 1270
BsmFI GGGAC 3 cut(s) 119, 1118, 1434
BsmI GAATGC 2 cut(s) 85, 646
BsnI GGCC 4 cut(s) 14, 416, 452, 1360
BsoBI CYCGRG 1 cut(s) 1101
Bsp119I TTCGAA 1 cut(s) 382
Bsp1286I GDGCHC 2 cut(s) 93, 313
Bsp13I TCCGGA 1 cut(s) 1042
Bsp143I GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
BspACI CCGC 5 cut(s) 709, 893, 1234, 1439, 1447
BspANI GGCC 4 cut(s) 14, 416, 452, 1360
BspEI TCCGGA 1 cut(s) 1042
BspFNI CGCG 1 cut(s) 1234
BspHI TCATGA 1 cut(s) 1366
BspLI GGNNCC 4 cut(s) 40, 327, 1106, 1118
BspPI GGATC 7 cut(s) 215, 269, 320, 333, 690, 1310, 1386
BspQI GCTCTTC 1 cut(s) 1437
BspT104I TTCGAA 1 cut(s) 382
BspT107I GGYRCC 2 cut(s) 38, 1116
BsrBI CCGCTC 2 cut(s) 1439, 1447
BsrDI GCAATG 2 cut(s) 95, 1302
BsrFI RCCGGY 1 cut(s) 1356
BsrI ACTGG 2 cut(s) 291, 322
BssAI RCCGGY 1 cut(s) 1356
BssECI CCNNGG 4 cut(s) 101, 102, 911, 1417
BssMI GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 4 cut(s) 715, 915, 1121, 1425
Bst6I CTCTTC 1 cut(s) 1437
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 3 cut(s) 603, 1202, 1214
BstBI TTCGAA 1 cut(s) 382
BstC8I GCNNGC 3 cut(s) 1009, 1341, 1391
BstDEI CTNAG 1 cut(s) 1385
BstDSI CCRYGG 1 cut(s) 911
BstF5I GGATG 5 cut(s) 364, 558, 871, 963, 1051
BstFNI CGCG 1 cut(s) 1234
BstHHI GCGC 1 cut(s) 1234
BstKTI GATC 9 cut(s) 223, 264, 328, 685, 1100, 1223, 1261, 1318, 1381
BstMAI GTCTC 1 cut(s) 1270
BstMBI GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
BstMWI GCNNNNNNNGC 6 cut(s) 97, 347, 603, 1004, 1202, 1214
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 3 cut(s) 29, 101, 1416
BstUI CGCG 1 cut(s) 1234
BstV1I GCAGC 4 cut(s) 235, 882, 1217, 1259
BstV2I GAAGAC 2 cut(s) 467, 1270
BstX2I RGATCY 3 cut(s) 325, 1315, 1378
BstYI RGATCY 3 cut(s) 325, 1315, 1378
BsuRI GGCC 4 cut(s) 14, 416, 452, 1360
BtgI CCRYGG 1 cut(s) 911
BtrI CACGTC 2 cut(s) 46, 1253
BtsCI GGATG 5 cut(s) 364, 558, 871, 963, 1051
BtsI GCAGTG 1 cut(s) 586
BtsIMutI CAGTG 2 cut(s) 586, 754
Cac8I GCNNGC 3 cut(s) 1009, 1341, 1391
CaiI CAGNNNCTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 1366
CfoI GCGC 1 cut(s) 1234
Cfr10I RCCGGY 1 cut(s) 1356
Cfr13I GGNCC 2 cut(s) 451, 1105
Csp6I GTAC 3 cut(s) 403, 1117, 1142
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 4 cut(s) 216, 663, 1224, 1367
CviQI GTAC 3 cut(s) 403, 1117, 1142
DdeI CTNAG 1 cut(s) 1385
DpnI GATC 9 cut(s) 222, 263, 327, 684, 1099, 1222, 1260, 1317, 1380
DpnII GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
Eam1104I CTCTTC 1 cut(s) 1437
EarI CTCTTC 1 cut(s) 1437
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 1105
Eco57I CTGAAG 3 cut(s) 461, 488, 1312
Eco88I CYCGRG 1 cut(s) 1101
EcoRII CCWGG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 4 cut(s) 219, 666, 1227, 1370
FaqI GGGAC 3 cut(s) 119, 1118, 1434
FatI CATG 4 cut(s) 215, 662, 1223, 1366
FauI CCCGC 2 cut(s) 1227, 1440
Fnu4HI GCNGC 5 cut(s) 249, 709, 896, 1206, 1248
FokI GGATG 5 cut(s) 371, 565, 878, 970, 1058
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 5 cut(s) 249, 709, 896, 1206, 1248
FspBI CTAG 2 cut(s) 51, 732
GlaI GCGC 1 cut(s) 1233
GluI GCNGC 5 cut(s) 249, 709, 896, 1206, 1248
GsuI CTGGAG 2 cut(s) 178, 455
HaeIII GGCC 4 cut(s) 14, 416, 452, 1360
HapII CCGG 4 cut(s) 30, 1043, 1357, 1417
HhaI GCGC 1 cut(s) 1234
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 4 cut(s) 219, 666, 1227, 1370
Hin6I GCGC 1 cut(s) 1232
HinP1I GCGC 1 cut(s) 1232
HindIII AAGCTT 1 cut(s) 1387
HinfI GANTC 3 cut(s) 163, 509, 977
HpaII CCGG 4 cut(s) 30, 1043, 1357, 1417
Hpy188I TCNGA 2 cut(s) 301, 957
Hpy99I CGWCG 5 cut(s) 25, 47, 59, 62, 957
HpyAV CCTTC 5 cut(s) 389, 938, 1074, 1154, 1314
HpyCH4III ACNGT 4 cut(s) 715, 915, 1121, 1425
HpyCH4IV ACGT 5 cut(s) 45, 57, 120, 737, 1252
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 347, 603, 1004, 1202, 1214
HpyF3I CTNAG 1 cut(s) 1385
HpySE526I ACGT 5 cut(s) 45, 57, 120, 737, 1252
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 4 cut(s) 219, 666, 1227, 1370
HspAI GCGC 1 cut(s) 1232
Kpn2I TCCGGA 1 cut(s) 1042
KpnI GGTACC 1 cut(s) 1120
Kzo9I GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
LguI GCTCTTC 1 cut(s) 1437
Lsp1109I GCAGC 4 cut(s) 235, 882, 1217, 1259
LweI GCATC 4 cut(s) 299, 349, 837, 1290
MaeI CTAG 2 cut(s) 51, 732
MaeII ACGT 5 cut(s) 45, 57, 120, 737, 1252
MaeIII GTNAC 6 cut(s) 116, 188, 628, 689, 751, 1425
MalI GATC 9 cut(s) 222, 263, 327, 684, 1099, 1222, 1260, 1317, 1380
MbiI CCGCTC 2 cut(s) 1439, 1447
MboI GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
MboII GAAGA 9 cut(s) 148, 467, 494, 800, 1190, 1275, 1373, 1424, 1454
MflI RGATCY 3 cut(s) 325, 1315, 1378
MhlI GDGCHC 2 cut(s) 93, 313
MmeI TCCRAC 2 cut(s) 935, 1356
MroI TCCGGA 1 cut(s) 1042
MseI TTAA 7 cut(s) 183, 210, 276, 353, 410, 483, 608
MslI CAYNNNNRTG 2 cut(s) 44, 551
MspA1I CMGCKG 1 cut(s) 895
MspI CCGG 4 cut(s) 30, 1043, 1357, 1417
MspR9I CCNGG 3 cut(s) 31, 103, 1418
Mva1269I GAATGC 2 cut(s) 85, 646
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 1234
MwoI GCNNNNNNNGC 6 cut(s) 97, 347, 603, 1004, 1202, 1214
NciI CCSGG 2 cut(s) 31, 1418
NdeII GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
NlaIII CATG 4 cut(s) 219, 666, 1227, 1370
NlaIV GGNNCC 4 cut(s) 40, 327, 1106, 1118
NmeAIII GCCGAG 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 116, 628
NspV TTCGAA 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 1366
PasI CCCWGGG 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 1437
PcsI WCGNNNNNNNCGW 4 cut(s) 42, 51, 1093, 1258
PctI GAATGC 2 cut(s) 85, 646
PfeI GAWTC 3 cut(s) 163, 509, 977
PkrI GCNGC 5 cut(s) 250, 710, 897, 1207, 1249
PshBI ATTAAT 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 4 cut(s) 40, 327, 1106, 1118
PspPI GGNCC 2 cut(s) 451, 1105
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 3 cut(s) 325, 1315, 1378
RsaI GTAC 3 cut(s) 404, 1118, 1143
RsaNI GTAC 3 cut(s) 403, 1117, 1142
RseI CAYNNNNRTG 2 cut(s) 44, 551
SapI GCTCTTC 1 cut(s) 1437
SaqAI TTAA 7 cut(s) 183, 210, 276, 353, 410, 483, 608
SatI GCNGC 5 cut(s) 249, 709, 896, 1206, 1248
Sau3AI GATC 9 cut(s) 220, 261, 325, 682, 1097, 1220, 1258, 1315, 1378
Sau96I GGNCC 2 cut(s) 451, 1105
ScrFI CCNGG 3 cut(s) 31, 103, 1418
SduI GDGCHC 2 cut(s) 93, 313
SfaNI GCATC 4 cut(s) 299, 349, 837, 1290
SfuI TTCGAA 1 cut(s) 382
SinI GGWCC 1 cut(s) 1105
SmiMI CAYNNNNRTG 2 cut(s) 44, 551
SmlI CTYRAG 1 cut(s) 1271
SmoI CTYRAG 1 cut(s) 1271
SsiI CCGC 5 cut(s) 709, 893, 1234, 1439, 1447
SspMI CTAG 2 cut(s) 51, 732
StyD4I CCNGG 3 cut(s) 29, 101, 1416
TaaI ACNGT 4 cut(s) 715, 915, 1121, 1425
TaiI ACGT 5 cut(s) 48, 60, 123, 740, 1255
TaqI TCGA 9 cut(s) 60, 157, 375, 382, 685, 1096, 1219, 1261, 1435
TauI GCSGC 1 cut(s) 711
TfiI GAWTC 3 cut(s) 163, 509, 977
Tru1I TTAA 7 cut(s) 183, 210, 276, 353, 410, 483, 608
Tru9I TTAA 7 cut(s) 183, 210, 276, 353, 410, 483, 608
TscAI CASTG 2 cut(s) 586, 754
TseFI GTSAC 2 cut(s) 116, 628
TseI GCWGC 4 cut(s) 248, 895, 1205, 1247
Tsp45I GTSAC 2 cut(s) 116, 628
TspDTI ATGAA 5 cut(s) 459, 651, 712, 969, 1383
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 2 cut(s) 586, 754
VpaK11BI GGWCC 1 cut(s) 1105
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 4 cut(s) 269, 520, 791, 1001
XspI CTAG 2 cut(s) 51, 732
ZraI GACGTC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.