AT1G18990

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011994
Physical Location & Seq
Reverse (-)
6558574 .. 6560561
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18990.1

Sequence Viewer

Length: 1575 bp
ATGTCTAACCGTTCCTTCAAGAATTTCATAGAAGAAGAACTTGGAACATTCCCACAGTTCTTAATCTACGCTCTTCTTGAATGGATCCTCATCATTATCCTCTTCATCGATGGCTTCCTCGCTTTCTTCTCTAACCAAATCGCTAAATTCTTTGACCTCAAAATCCCCTGTCTTCTCTGCACTAGACTCGACCATGTTCTTGTCAGCAGAAACCCTGATTTCTATTACAACGACTCGATATGCGATGCTCACAAGAAGAATGTCTCATCTCTCGCTTACTGTCATGTGCACAAGAAACTCTCTGAGATCAAACGTATGTGTGAAGGATGTCTTCTCTCTTTCGCTACCGAGAAAGAAACAGATGTTGATACTTACAAGTCTCTTATTGGAATCTTGCATAAGGATCTTGAGCTTCTTATCGACGATGAACGTGAGCTTCAATTGGCGTTTCCGGTTGCGGGTTCGAAGAAAGATGAAAACTTCTATCAGGTTGAGAACAGAACTAACAATAGCAATGACAGGTTTCAGAGGCAGCAACGTTGTTCTTGTTGTGGGCAGATAATGAAGCTGAAATCCGATAAACCTAAGAGCAATAATCAATCTTTTTTCGGAGCTCCTAGTCCATCTCCTAGAGTTTCATTTAACCAAAGAACTTTGGATTTGTCTAACATTAAATACACGGATTTGCCTGAGGATGATGATGCTTTGAACACGAAAGGCGCTTCGTTGGATGCAGTTGATGACAGAACTCCGAGTTTTGTAAAAGGAGGTAATAAGTTCTTTGGCATTCCGCTTTCTGACTCGGCTCAGAATAGTCCGCGATGGTCTGTTCGATCAATGAAGAAATCTTTCGTTGATCAGAATGGTTTGGAGAGCGAAGTGTTGGATGGAGATTCGATACTTCAGCATCTAAATAGACAGGTTAGATTGGATCGCAAGTCGTTGATGGATTTGTATATGGAGTTAGATGAAGAGAGGAGTGCTTCAGCTGTTGCAGCAAACAATGCAATGGCTATGATCACAAGACTTCAAGCAGAGAAAGCTGCGGTTCAAATGGAGGCATTGCAGTATCAGAGAATGATGGATGAGCAAGCAGAATATGATCAAGAAGCTTTGCAGTCTATGAATGGTTTGTTGGTTAAGAGAGAAGAGGAAATGAAAGAGCTTGAAGCTGGAATTGAAGTTTATAGATTGAGATATGGATTGTTGAGAGAAGAAAGAGGAGAAGCAGAGGAGTTTCTTGATGAAGAAACTAAACCGGTTTCAGATTTGCCTGTTTGTTCTTCAAATCATGAAGAAGATTTGGAACAAATGAAAGATTCAGCTGAGGATTCTATAGGCAATAATGGTGTGATGATTATAGAGGAAGAAAAAGAGAATGGAAGCAGAAAAGATATGTTAGTGAAGGAAATTTCAGAGATTACAGAGAGACTAAATGCAATTGAATCAAAAGGTGAATTGTTGCAGCAAATTTCAGATGTTTTAGATGTTAGTGAAGGAGAAGCAATTCTATTACAGATATCTCAGAATCTGCATATGCTTCGTAGTTTCATTGAAATGCCAACAGAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

524

Amino Acids

59.88

Weight (kDa)

4.75

Isoelectric Point (pI)

55.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 303 - 391 1.6e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 820
AciI CCGC 4 cut(s) 458, 791, 818, 1046
AclI AACGTT 1 cut(s) 538
AclWI GGATC 4 cut(s) 79, 92, 411, 939
AcsI RAATTY 4 cut(s) 22, 146, 1410, 1470
AcuI CTGAAG 2 cut(s) 887, 969
AfiI CCNNNNNNNGG 1 cut(s) 458
AgeI ACCGGT 1 cut(s) 1258
AjuI GAANNNNNNNTTGG 4 cut(s) 24, 56, 1118, 1150
Alw21I GWGCWC 2 cut(s) 291, 616
Alw26I GTCTC 3 cut(s) 268, 384, 1423
Alw44I GTGCAC 1 cut(s) 287
AlwI GGATC 4 cut(s) 79, 92, 411, 939
AlwNI CAGNNNCTG 1 cut(s) 1531
ApaLI GTGCAC 1 cut(s) 287
ApeKI GCWGC 4 cut(s) 532, 995, 1043, 1465
ApoI RAATTY 4 cut(s) 22, 146, 1410, 1470
AsiGI ACCGGT 1 cut(s) 1258
Asp700I GAANNNNTTC 2 cut(s) 848, 1506
AspLEI GCGC 1 cut(s) 722
AsuHPI GGTGA 1 cut(s) 1466
AsuII TTCGAA 1 cut(s) 464
AxyI CCTNAGG 1 cut(s) 690
BaeGI GKGCMC 1 cut(s) 291
BamHI GGATCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 616
BbsI GAAGAC 2 cut(s) 164, 323
Bbv12I GWGCWC 2 cut(s) 291, 616
BbvCI CCTCAGC 1 cut(s) 1326
BbvI GCAGC 4 cut(s) 544, 1007, 1030, 1477
BccI CCATC 6 cut(s) 104, 631, 816, 881, 940, 1075
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 1523, 1557
BclI TGATCA 3 cut(s) 856, 1017, 1102
BcoDI GTCTC 3 cut(s) 268, 384, 1423
BfaI CTAG 3 cut(s) 183, 618, 630
BfmI CTRYAG 1 cut(s) 1335
BfoI RGCGCY 1 cut(s) 723
BisI GCNGC 4 cut(s) 533, 996, 1044, 1466
BlsI GCNGC 4 cut(s) 534, 997, 1045, 1467
BmiI GGNNCC 1 cut(s) 86
BmsI GCATC 4 cut(s) 235, 691, 721, 916
BpiI GAAGAC 2 cut(s) 164, 323
Bpu10I CCTNAGC 1 cut(s) 1326
Bpu14I TTCGAA 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 428
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 89
BsaWI WCCGGW 2 cut(s) 451, 1258
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 1 cut(s) 1258
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 3 cut(s) 520, 1014, 1061
Bse8I GATNNNNATC 1 cut(s) 89
BseCI ATCGAT 1 cut(s) 108
BseGI GGATG 5 cut(s) 332, 700, 736, 892, 1090
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 3 cut(s) 520, 1014, 1061
BseMII CTCAG 5 cut(s) 294, 681, 821, 1317, 1538
BseRI GAGGAG 3 cut(s) 991, 1236, 1247
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 4 cut(s) 544, 1007, 1030, 1477
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 820
BshTI ACCGGT 1 cut(s) 1258
BshVI ATCGAT 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 291, 616
BsiSI CCGG 2 cut(s) 452, 1259
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 3 cut(s) 268, 384, 1423
BsmI GAATGC 1 cut(s) 786
Bsp119I TTCGAA 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 291, 616
Bsp143I GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
BspACI CCGC 4 cut(s) 458, 791, 818, 1046
BspCNI CTCAG 5 cut(s) 295, 682, 820, 1318, 1537
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 1 cut(s) 820
BspHI TCATGA 2 cut(s) 1291, 1571
BspLI GGNNCC 1 cut(s) 86
BspPI GGATC 4 cut(s) 79, 92, 411, 939
BspQI GCTCTTC 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 464
BsrDI GCAATG 3 cut(s) 520, 1014, 1061
BsrFI RCCGGY 1 cut(s) 1258
BssAI RCCGGY 1 cut(s) 1258
BssMI GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
Bst4CI ACNGT 3 cut(s) 11, 57, 281
Bst6I CTCTTC 4 cut(s) 78, 107, 966, 1143
BstAPI GCANNNNNTGC 1 cut(s) 1004
BstBI TTCGAA 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 1092
BstDEI CTNAG 6 cut(s) 303, 585, 690, 807, 1326, 1524
BstF5I GGATG 5 cut(s) 332, 700, 736, 892, 1090
BstFNI CGCG 1 cut(s) 820
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 8 cut(s) 87, 309, 406, 836, 859, 934, 1020, 1105
BstMAI GTCTC 3 cut(s) 268, 384, 1423
BstMBI GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 995, 1004, 1040
BstSFI CTRYAG 1 cut(s) 1335
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 820
BstV1I GCAGC 4 cut(s) 544, 1007, 1030, 1477
BstV2I GAAGAC 2 cut(s) 164, 323
BstX2I RGATCY 2 cut(s) 84, 403
BstYI RGATCY 2 cut(s) 84, 403
Bsu15I ATCGAT 1 cut(s) 108
Bsu36I CCTNAGG 1 cut(s) 690
BsuTUI ATCGAT 1 cut(s) 108
BtgZI GCGATG 2 cut(s) 258, 835
BtsCI GGATG 5 cut(s) 332, 700, 736, 892, 1090
Cac8I GCNNGC 1 cut(s) 1092
CaiI CAGNNNCTG 1 cut(s) 1531
CciI TCATGA 2 cut(s) 1291, 1571
CfoI GCGC 1 cut(s) 722
Cfr10I RCCGGY 1 cut(s) 1258
ClaI ATCGAT 1 cut(s) 108
CspAI ACCGGT 1 cut(s) 1258
CviAII CATG 4 cut(s) 194, 284, 1292, 1572
DdeI CTNAG 6 cut(s) 303, 585, 690, 807, 1326, 1524
DpnI GATC 8 cut(s) 86, 308, 405, 835, 858, 933, 1019, 1104
DpnII GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
Eam1104I CTCTTC 4 cut(s) 78, 107, 966, 1143
EarI CTCTTC 4 cut(s) 78, 107, 966, 1143
Ecl136II GAGCTC 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 616
Eco32I GATATC 1 cut(s) 1521
Eco53kI GAGCTC 1 cut(s) 614
Eco57I CTGAAG 2 cut(s) 887, 969
Eco81I CCTNAGG 1 cut(s) 690
EcoICRI GAGCTC 1 cut(s) 614
EcoRV GATATC 1 cut(s) 1521
EcoT38I GRGCYC 1 cut(s) 616
FaeI CATG 4 cut(s) 197, 287, 1295, 1575
FalI AAGNNNNNCTT 4 cut(s) 24, 56, 315, 347
FatI CATG 4 cut(s) 193, 283, 1291, 1571
FauI CCCGC 1 cut(s) 451
FauNDI CATATG 1 cut(s) 1536
FbaI TGATCA 3 cut(s) 856, 1017, 1102
Fnu4HI GCNGC 4 cut(s) 533, 996, 1044, 1466
FokI GGATG 5 cut(s) 339, 707, 743, 899, 1097
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 4 cut(s) 533, 996, 1044, 1466
FspBI CTAG 3 cut(s) 183, 618, 630
GlaI GCGC 1 cut(s) 721
GluI GCNGC 4 cut(s) 533, 996, 1044, 1466
HaeII RGCGCY 1 cut(s) 723
HapII CCGG 2 cut(s) 452, 1259
HhaI GCGC 1 cut(s) 722
Hin1II CATG 4 cut(s) 197, 287, 1295, 1575
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HindIII AAGCTT 1 cut(s) 1110
HpaII CCGG 2 cut(s) 452, 1259
HphI GGTGA 1 cut(s) 1466
Hpy166II GTNNAC 1 cut(s) 289
Hpy188III TCNNGA 7 cut(s) 19, 77, 407, 1106, 1241, 1292, 1572
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 4 cut(s) 25, 317, 1399, 1490
HpyCH4III ACNGT 3 cut(s) 11, 57, 281
HpyCH4IV ACGT 3 cut(s) 313, 430, 538
HpyF10VI GCNNNNNNNGC 3 cut(s) 995, 1004, 1040
HpyF3I CTNAG 6 cut(s) 303, 585, 690, 807, 1326, 1524
HpySE526I ACGT 3 cut(s) 313, 430, 538
Hsp92II CATG 4 cut(s) 197, 287, 1295, 1575
HspAI GCGC 1 cut(s) 720
Ksp22I TGATCA 3 cut(s) 856, 1017, 1102
Kzo9I GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
LguI GCTCTTC 1 cut(s) 78
LmnI GCTCC 2 cut(s) 611, 619
Lsp1109I GCAGC 4 cut(s) 544, 1007, 1030, 1477
LweI GCATC 4 cut(s) 235, 691, 721, 916
MaeI CTAG 3 cut(s) 183, 618, 630
MaeII ACGT 3 cut(s) 313, 430, 538
MalI GATC 8 cut(s) 86, 308, 405, 835, 858, 933, 1019, 1104
MboI GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
MfeI CAATTG 2 cut(s) 440, 1440
MflI RGATCY 2 cut(s) 84, 403
MhlI GDGCHC 2 cut(s) 291, 616
MluCI AATT 9 cut(s) 22, 146, 440, 1176, 1410, 1440, 1457, 1470, 1506
MlyI GAGTC 3 cut(s) 180, 227, 794
MmeI TCCRAC 2 cut(s) 708, 864
MroXI GAANNNNTTC 2 cut(s) 848, 1506
MseI TTAA 4 cut(s) 62, 642, 672, 1140
MslI CAYNNNNRTG 1 cut(s) 1556
MspA1I CMGCKG 2 cut(s) 989, 1325
MspI CCGG 2 cut(s) 452, 1259
MunI CAATTG 2 cut(s) 440, 1440
Mva1269I GAATGC 1 cut(s) 786
MvnI CGCG 1 cut(s) 820
MwoI GCNNNNNNNGC 3 cut(s) 995, 1004, 1040
NdeI CATATG 1 cut(s) 1536
NdeII GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
NlaIII CATG 4 cut(s) 197, 287, 1295, 1575
NlaIV GGNNCC 1 cut(s) 86
NmeAIII GCCGAG 1 cut(s) 782
NspV TTCGAA 1 cut(s) 464
PagI TCATGA 2 cut(s) 1291, 1571
PciSI GCTCTTC 1 cut(s) 78
PctI GAATGC 1 cut(s) 786
PdmI GAANNNNTTC 2 cut(s) 848, 1506
PfeI GAWTC 7 cut(s) 390, 893, 1319, 1331, 1445, 1528, 1568
PinAI ACCGGT 1 cut(s) 1258
PkrI GCNGC 4 cut(s) 534, 997, 1045, 1467
PleI GAGTC 3 cut(s) 180, 227, 794
PpsI GAGTC 3 cut(s) 180, 227, 794
Psp124BI GAGCTC 1 cut(s) 616
Psp1406I AACGTT 1 cut(s) 538
PspN4I GGNNCC 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 1531
PsuI RGATCY 2 cut(s) 84, 403
PvuII CAGCTG 2 cut(s) 989, 1325
RseI CAYNNNNRTG 1 cut(s) 1556
SacI GAGCTC 1 cut(s) 616
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 4 cut(s) 62, 642, 672, 1140
SatI GCNGC 4 cut(s) 533, 996, 1044, 1466
Sau3AI GATC 8 cut(s) 84, 306, 403, 833, 856, 931, 1017, 1102
SchI GAGTC 3 cut(s) 180, 227, 794
SduI GDGCHC 2 cut(s) 291, 616
SfaNI GCATC 4 cut(s) 235, 691, 721, 916
SfcI CTRYAG 1 cut(s) 1335
SfuI TTCGAA 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 1556
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 9 cut(s) 22, 146, 440, 1176, 1410, 1440, 1457, 1470, 1506
SsiI CCGC 4 cut(s) 458, 791, 818, 1046
SspMI CTAG 3 cut(s) 183, 618, 630
SstI GAGCTC 1 cut(s) 616
TaaI ACNGT 3 cut(s) 11, 57, 281
TaiI ACGT 3 cut(s) 316, 433, 541
TaqI TCGA 7 cut(s) 108, 189, 236, 420, 464, 832, 896
TasI AATT 9 cut(s) 22, 146, 440, 1176, 1410, 1440, 1457, 1470, 1506
TfiI GAWTC 7 cut(s) 390, 893, 1319, 1331, 1445, 1528, 1568
Tru1I TTAA 4 cut(s) 62, 642, 672, 1140
Tru9I TTAA 4 cut(s) 62, 642, 672, 1140
TseI GCWGC 4 cut(s) 532, 995, 1043, 1465
TspGWI ACGGA 1 cut(s) 695
VneI GTGCAC 1 cut(s) 287
XapI RAATTY 4 cut(s) 22, 146, 1410, 1470
XmnI GAANNNNTTC 2 cut(s) 848, 1506
XspI CTAG 3 cut(s) 183, 618, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.