Rh7BG030400

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011994
Physical Location & Seq
Forward (+)
2057632 .. 2060486
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG030400.1

Sequence Viewer

Length: 2592 bp
ATGAGTTCAAACAAGAAAACAATGCCAAAGGGACGCTTCACCCGTTATGTGGAGCAAAACATGGGAGAGCTCTCACGCTTCATGGTCTATGCTGTGCTTGAATGGGTCTTGATCATTCTGCTCTTCATTGATGGCTTTTTTGCTTTTGTTGCCAATGAATTCGCCAAGCTATTTGAGCTCAGATACCCCTGCTGGCTTTGCACCAGGATCGATCACATTCTCGTTAACAGAGGTCGTGATTTCTATTATAATGATTCCATATGTGAATCACACAAGAAAGATGTCTCGTACCTTGCTTATTGCCACAACCACAAGAGGCTATCTGACATAAGAAAGATGTGTGAGGCATGTCTTCTTTCATTTGCAACCGAAAAAGAGTCTGATTTGGACACATACAAGTCCCTAGTTGGGATCTTACACAAGGATCTCGAGTGCTTGGTGGAGGATGATGAACATCAAATTCAGCTGAGTGTGCCTGCGGCAAGGACATGGGAGGATGCAGGGTCGATTGAGAAGAGTAGGGACAGCATGACCATAGGGTGTTCGTGTTGCGGTGCGCCATTGAAGTTGAGTTCAGCTTCCTCCTACTCAAAAGCAAAGAGTGTTAGCGGGCTAGCGCAAGCTCCTACGCCTTCTCCACGAGCACCCCCAGGGAGAAATGATGATAATCCTGGTTTGGACTTGCCCCCTATTCGATACCCAGAACTCAAGTTTATGTCTGATAATGAGTCTGAGCTTCCAGAGGATGAGTATGGAACACATACGAAAAATCAAAAGGAAGATCCGAAAGCTGCTACATTGCCATTATTGTCAGAAGGTGATGAGTTAACTGATGATGCTAATAGGACTCCTATATATGGCAGAGGAAACAGGTTCTTTGGAATCCCACTTTCAGATTCAGCTCAAAATAGTCCTAGGGTGGCTATGAGGATTTCAAAGAAATCACCACTCGAAAAGAGCGAGTTTGCTTTGGAATCGGTTGATGGAAATCTTCCAAATGATGCGGATTGTGATTCAATTTTGCACCGTCTGAAGAGACAGGTCCGCGTGGACAGGAAGTCACTGATGGCTTTGTACATGGAACTCGATGAAGAAAGAAGTGCTTCAGCTGTAGCAGCCAACAATGCAATGGCTATGATCACAAGGCTACAAGCAGAGAAGGCTGCTGTTCAGATGGAGGCATTGCAGTATCAGAGAATGATGGAGGAGCAGGCGGAGTATGACCAAGAAGCCCTGGAAGCAACATATGATTTGCTTGCCAAGAGAGAAGATGATCTCAGGGTTTTAGAGGCTGAAATAGAGGCTTATAGGGAGAAATATGGATTGTTGAAGGAATTTGTCTACAAGGACCAAGATGAGGCTGGTGAATGTGGTACTCCTCTAAGCGTAAAGTATGGCGCAAACAACATGCAAAATGATCAAGCCGGGTCAGCAAAGGAGGAGAATGCAGACGCTGCCCTCGGTGAAAGTTTGAAACCACTGAAAGGGGAGAAGATTTACCTACTTGGGCGGTTCAAGAAAATGGACAAGATGAGAAACTTATCATCTAATGGGTTCTTGCCAGATGGTCTGAATGAGGATGAGATAGGAAATGGAAACAAAGCTGCCCTAACAAGGCAATTGTCGCTGTTGTCTCATCTTAGTGATAGAGTGAAGGCTCTTGAATCTGACACTGGATTCTTAGAGTATGCTGCTAAGACACTTGAGAAACATAGTGAAGGAACAAAACTTTTGACAGAGATAACTCAGAATTTACAGAAGCTCCGGCACCTTGTGAATCTTCCTACTGAGGAACAAAACAAGACGCCTCCAGCGGAACCAAAAAAGGAGCAAAACAAGACACCTTCTGAGGAAAAAAAGAAGGAGCAAAACAAGACACATCCGGAGGACCAAAAGAAGGAGCAAAGTAAGACGCATTCGGAGGAACAAAAGGAGCAAAACAAGACGCCTCCGGAGGAACAAATTCAAAGTAAGACGCCTGTAGAGGAACCAAAGAATGAGCAAAGTAAGACGCCTCCAGAGGAACAAAAGAAGGAGCAAACCAAGACGCCTCCAGAGGAACAAAAGAACGAGCAAAGTAAGACGCCTATGGAGGAACAAAAGAAGGAGCAAAACAAGACGTCTCCAGACAAACAAAATAAGACGCATCCGGAGGAACAAAAGGAACAAAATAATACACCTGCGGAGGAACAAAAGGAGCAAAACAAGATGCCTCCGGAGGAACAAAAGAAGGAGCAAAACAAGACGCCTCCAGAGGAACAAAAGAATGAGCAAAGTAAGACGCCTTCGGAGGACAAAAGTAAGACGCCTCTGGAGGAACAAAAGAAGGAGCAAAACGATACGCCTCTGGAGGAACAAAAGAAAGAGCAAAGTAAGACACATCCGGAGGAACAAAAGAATGAGCAAAGTAAGACACCTCCGGAGGAACAAAAGAAGCAAAACAAGACGCCTTCGGAGGAACAAAATGAGCAAAAGAAAACGCTTTCGGATGAACAAAATAAGATTCCTCCAGAAGAACTAAACAAGACATCTCCAGCAGAACAGAAAAAGGAACAAAAAGACTTTACTTCCATAGTAAAAAGTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

863

Amino Acids

98.53

Weight (kDa)

5.3

Isoelectric Point (pI)

63.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 343 - 433 1.4e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AarI CACCTGC 1 cut(s) 2188
AatII GACGTC 1 cut(s) 2123
Acc36I ACCTGC 1 cut(s) 2188
AccB1I GGYRCC 1 cut(s) 1767
AccI GTMKAC 1 cut(s) 1341
AccII CGCG 1 cut(s) 1047
AccIII TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
AciI CCGC 9 cut(s) 479, 552, 609, 1004, 1045, 1214, 1510, 1814, 2183
AclWI GGATC 4 cut(s) 215, 419, 432, 776
AcsI RAATTY 5 cut(s) 158, 459, 1334, 1750, 1962
AcuI CTGAAG 2 cut(s) 1052, 1089
AdeI CACNNNGTG 1 cut(s) 1774
AfaI GTAC 3 cut(s) 290, 1076, 1375
AfiI CCNNNNNNNGG 7 cut(s) 49, 408, 857, 1357, 1484, 1614, 1897
AhdI GACNNNNNGTC 1 cut(s) 1057
AjnI CCWGG 4 cut(s) 203, 649, 670, 1233
Alw21I GWGCWC 3 cut(s) 72, 180, 646
Alw26I GTCTC 4 cut(s) 289, 1030, 1638, 2127
AlwI GGATC 4 cut(s) 215, 419, 432, 776
AlwNI CAGNNNCTG 1 cut(s) 1454
Ama87I CYCGRG 1 cut(s) 428
Aor13HI TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
ApeKI GCWGC 6 cut(s) 791, 1115, 1163, 1454, 1604, 1691
ApoI RAATTY 5 cut(s) 158, 459, 1334, 1750, 1962
Asp700I GAANNNNTTC 2 cut(s) 1102, 1962
AspA2I CCTAGG 1 cut(s) 914
AspLEI GCGC 3 cut(s) 559, 619, 1400
AspS9I GGNCC 3 cut(s) 1042, 1348, 1888
AsuC2I CCSGG 1 cut(s) 1426
AsuHPI GGTGA 5 cut(s) 31, 830, 936, 1376, 1475
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 3 cut(s) 1042, 1348, 1888
AvrII CCTAGG 1 cut(s) 914
BanI GGYRCC 1 cut(s) 1767
BanII GRGCYC 2 cut(s) 72, 180
BarI GAAGNNNNNNTAC 2 cut(s) 2269, 2301
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 3 cut(s) 72, 180, 646
BbvI GCAGC 6 cut(s) 778, 1127, 1150, 1441, 1591, 1678
BccI CCATC 6 cut(s) 125, 977, 1060, 1168, 1195, 1559
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 4 cut(s) 205, 651, 672, 1235
BclI TGATCA 3 cut(s) 111, 1137, 1417
BcnI CCSGG 1 cut(s) 1426
BcoDI GTCTC 4 cut(s) 289, 1030, 1638, 2127
BfaI CTAG 3 cut(s) 404, 614, 915
BfmI CTRYAG 2 cut(s) 1110, 1980
BfuAI ACCTGC 1 cut(s) 2188
BisI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
BlnI CCTAGG 1 cut(s) 914
BlsI GCNGC 7 cut(s) 481, 793, 1117, 1165, 1456, 1606, 1693
Bme1390I CCNGG 5 cut(s) 205, 651, 672, 1235, 1426
Bme18I GGWCC 3 cut(s) 1042, 1348, 1888
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 1042, 1348, 1888
BmiI GGNNCC 3 cut(s) 1769, 1818, 1989
BmrFI CCNGG 5 cut(s) 205, 651, 672, 1235, 1426
BmsI GCATC 5 cut(s) 487, 826, 991, 2155, 2199
BmtI GCTAGC 1 cut(s) 617
BpiI GAAGAC 1 cut(s) 344
BpmI CTGGAG 9 cut(s) 1794, 2001, 2037, 2109, 2235, 2333, 2369, 2493, 2517
BpuEI CTTGAG 2 cut(s) 692, 1724
BpuMI CCSGG 1 cut(s) 1426
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 3 cut(s) 453, 666, 987
BsaJI CCNNGG 5 cut(s) 649, 650, 914, 1233, 1459
BsaWI WCCGGW 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 1746, 1776
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 408, 857, 1357, 1484, 1614, 1897
Bse1I ACTGG 1 cut(s) 1678
Bse3DI GCAATG 3 cut(s) 797, 1134, 1181
Bse8I GATNNNNATC 3 cut(s) 453, 666, 987
BseAI TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
BseBI CCWGG 4 cut(s) 205, 651, 672, 1235
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 5 cut(s) 649, 650, 914, 1233, 1459
BseGI GGATG 8 cut(s) 451, 502, 751, 1585, 1879, 2146, 2380, 2494
BseJI GATNNNNATC 3 cut(s) 453, 666, 987
BseLI CCNNNNNNNGG 7 cut(s) 49, 408, 857, 1357, 1484, 1614, 1897
BseMI GCAATG 3 cut(s) 797, 1134, 1181
BseMII CTCAG 7 cut(s) 193, 458, 723, 1291, 1760, 1779, 1839
BseNI ACTGG 1 cut(s) 1678
BseRI GAGGAG 3 cut(s) 1220, 1368, 1454
BseXI GCAGC 6 cut(s) 778, 1127, 1150, 1441, 1591, 1678
Bsh1236I CGCG 1 cut(s) 1047
BshNI GGYRCC 1 cut(s) 1767
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 3 cut(s) 72, 180, 646
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 8 cut(s) 1425, 1765, 1883, 1952, 2150, 2216, 2384, 2420
BslFI GGGAC 3 cut(s) 45, 385, 536
BslI CCNNNNNNNGG 7 cut(s) 49, 408, 857, 1357, 1484, 1614, 1897
BsmAI GTCTC 4 cut(s) 289, 1030, 1638, 2127
BsmBI CGTCTC 1 cut(s) 2127
BsmFI GGGAC 3 cut(s) 45, 385, 536
BsmI GAATGC 2 cut(s) 1450, 1915
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 72, 180, 646
Bsp13I TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
Bsp1407I TGTACA 1 cut(s) 1074
Bsp143I GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
BspACI CCGC 9 cut(s) 479, 552, 609, 1004, 1045, 1214, 1510, 1814, 2183
BspCNI CTCAG 7 cut(s) 192, 459, 724, 1290, 1759, 1780, 1840
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
BspFNI CGCG 1 cut(s) 1047
BspLI GGNNCC 3 cut(s) 1769, 1818, 1989
BspMI ACCTGC 1 cut(s) 2188
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 4 cut(s) 215, 419, 432, 776
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 1767
BsrDI GCAATG 3 cut(s) 797, 1134, 1181
BsrGI TGTACA 1 cut(s) 1074
BsrI ACTGG 1 cut(s) 1678
BssECI CCNNGG 5 cut(s) 649, 650, 914, 1233, 1459
BssMI GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 914
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 4 cut(s) 205, 651, 672, 1235
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 3 cut(s) 128, 509, 1028
BstAPI GCANNNNNTGC 1 cut(s) 1454
BstAUI TGTACA 1 cut(s) 1074
BstC8I GCNNGC 7 cut(s) 194, 477, 611, 615, 621, 1212, 1257
BstF5I GGATG 8 cut(s) 451, 502, 751, 1585, 1879, 2146, 2380, 2494
BstFNI CGCG 1 cut(s) 1047
BstHHI GCGC 3 cut(s) 559, 619, 1400
BstKTI GATC 9 cut(s) 114, 210, 214, 414, 427, 784, 1140, 1276, 1420
BstMAI GTCTC 4 cut(s) 289, 1030, 1638, 2127
BstMBI GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
BstNI CCWGG 4 cut(s) 205, 651, 672, 1235
BstNSI RCATGY 2 cut(s) 351, 1411
BstSCI CCNGG 5 cut(s) 203, 649, 670, 1233, 1424
BstSFI CTRYAG 2 cut(s) 1110, 1980
BstUI CGCG 1 cut(s) 1047
BstV1I GCAGC 6 cut(s) 778, 1127, 1150, 1441, 1591, 1678
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 3 cut(s) 411, 424, 781
BstYI RGATCY 3 cut(s) 411, 424, 781
Bsu15I ATCGAT 1 cut(s) 210
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 8 cut(s) 451, 502, 751, 1585, 1879, 2146, 2380, 2494
BtsIMutI CAGTG 3 cut(s) 1061, 1478, 1671
BveI ACCTGC 1 cut(s) 2188
Cac8I GCNNGC 7 cut(s) 194, 477, 611, 615, 621, 1212, 1257
CaiI CAGNNNCTG 1 cut(s) 1454
CfoI GCGC 3 cut(s) 559, 619, 1400
Cfr13I GGNCC 3 cut(s) 1042, 1348, 1888
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 3 cut(s) 289, 1075, 1374
CviAII CATG 7 cut(s) 61, 82, 348, 489, 529, 1078, 1408
CviQI GTAC 3 cut(s) 289, 1075, 1374
DpnI GATC 9 cut(s) 113, 209, 213, 413, 426, 783, 1139, 1275, 1419
DpnII GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
DraIII CACNNNGTG 1 cut(s) 1774
DriI GACNNNNNGTC 1 cut(s) 1057
Eam1104I CTCTTC 3 cut(s) 128, 509, 1028
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 3 cut(s) 128, 509, 1028
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 2 cut(s) 70, 178
Eco130I CCWWGG 1 cut(s) 914
Eco24I GRGCYC 2 cut(s) 72, 180
Eco47I GGWCC 3 cut(s) 1042, 1348, 1888
Eco53kI GAGCTC 2 cut(s) 70, 178
Eco57I CTGAAG 2 cut(s) 1052, 1089
Eco88I CYCGRG 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 70, 178
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 4 cut(s) 203, 649, 670, 1233
EcoT14I CCWWGG 1 cut(s) 914
EcoT38I GRGCYC 2 cut(s) 72, 180
ErhI CCWWGG 1 cut(s) 914
Esp3I CGTCTC 1 cut(s) 2127
FaeI CATG 7 cut(s) 64, 85, 351, 492, 532, 1081, 1411
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 1087, 1119
FaqI GGGAC 3 cut(s) 45, 385, 536
FatI CATG 7 cut(s) 60, 81, 347, 488, 528, 1077, 1407
FauI CCCGC 1 cut(s) 602
FauNDI CATATG 2 cut(s) 260, 1246
FbaI TGATCA 3 cut(s) 111, 1137, 1417
FblI GTMKAC 1 cut(s) 1341
Fnu4HI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
FokI GGATG 8 cut(s) 458, 509, 758, 1592, 1866, 2133, 2367, 2501
FriOI GRGCYC 2 cut(s) 72, 180
Fsp4HI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
FspBI CTAG 3 cut(s) 404, 614, 915
GlaI GCGC 3 cut(s) 558, 618, 1399
GluI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
GsuI CTGGAG 9 cut(s) 1794, 2001, 2037, 2109, 2235, 2333, 2369, 2493, 2517
HapII CCGG 8 cut(s) 1425, 1765, 1883, 1952, 2150, 2216, 2384, 2420
HhaI GCGC 3 cut(s) 559, 619, 1400
Hin1II CATG 7 cut(s) 64, 85, 351, 492, 532, 1081, 1411
Hin6I GCGC 3 cut(s) 557, 617, 1398
HinP1I GCGC 3 cut(s) 557, 617, 1398
HincII GTYRAC 2 cut(s) 226, 828
HindII GTYRAC 2 cut(s) 226, 828
HpaI GTTAAC 2 cut(s) 226, 828
HpaII CCGG 8 cut(s) 1425, 1765, 1883, 1952, 2150, 2216, 2384, 2420
HphI GGTGA 5 cut(s) 31, 830, 936, 1376, 1475
Hpy166II GTNNAC 4 cut(s) 226, 828, 1051, 1342
Hpy8I GTNNAC 4 cut(s) 226, 828, 1051, 1342
HpyCH4III ACNGT 1 cut(s) 1028
HpyCH4IV ACGT 1 cut(s) 2120
HpyCH4V TGCA 8 cut(s) 201, 365, 500, 1024, 1127, 1186, 1411, 1448
HpySE526I ACGT 1 cut(s) 2120
Hsp92II CATG 7 cut(s) 64, 85, 351, 492, 532, 1081, 1411
HspAI GCGC 3 cut(s) 557, 617, 1398
Kpn2I TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
Ksp22I TGATCA 3 cut(s) 111, 1137, 1417
KspAI GTTAAC 2 cut(s) 226, 828
Kzo9I GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
LguI GCTCTTC 1 cut(s) 128
Lsp1109I GCAGC 6 cut(s) 778, 1127, 1150, 1441, 1591, 1678
LweI GCATC 5 cut(s) 487, 826, 991, 2155, 2199
MaeI CTAG 3 cut(s) 404, 614, 915
MaeII ACGT 1 cut(s) 2120
MaeIII GTNAC 1 cut(s) 1059
MalI GATC 9 cut(s) 113, 209, 213, 413, 426, 783, 1139, 1275, 1419
MboI GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
MfeI CAATTG 1 cut(s) 1619
MflI RGATCY 3 cut(s) 411, 424, 781
MhlI GDGCHC 3 cut(s) 72, 180, 646
MluCI AATT 7 cut(s) 158, 459, 1017, 1334, 1619, 1750, 1962
MlyI GAGTC 3 cut(s) 386, 737, 841
MroI TCCGGA 6 cut(s) 1882, 1951, 2149, 2215, 2383, 2419
MroXI GAANNNNTTC 2 cut(s) 1102, 1962
MseI TTAA 2 cut(s) 225, 827
MslI CAYNNNNRTG 1 cut(s) 1641
MspA1I CMGCKG 3 cut(s) 466, 1109, 1814
MspI CCGG 8 cut(s) 1425, 1765, 1883, 1952, 2150, 2216, 2384, 2420
MspR9I CCNGG 5 cut(s) 205, 651, 672, 1235, 1426
MunI CAATTG 1 cut(s) 1619
Mva1269I GAATGC 2 cut(s) 1450, 1915
MvaI CCWGG 4 cut(s) 205, 651, 672, 1235
MvnI CGCG 1 cut(s) 1047
NciI CCSGG 1 cut(s) 1426
NdeI CATATG 2 cut(s) 260, 1246
NdeII GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 7 cut(s) 64, 85, 351, 492, 532, 1081, 1411
NlaIV GGNNCC 3 cut(s) 1769, 1818, 1989
NmuCI GTSAC 1 cut(s) 1059
NspI RCATGY 2 cut(s) 351, 1411
PaeR7I CTCGAG 1 cut(s) 428
PaqCI CACCTGC 1 cut(s) 2188
PasI CCCWGGG 1 cut(s) 650
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 957
PctI GAATGC 2 cut(s) 1450, 1915
PdmI GAANNNNTTC 2 cut(s) 1102, 1962
PkrI GCNGC 7 cut(s) 481, 793, 1117, 1165, 1456, 1606, 1693
PleI GAGTC 3 cut(s) 385, 736, 841
PpsI GAGTC 3 cut(s) 385, 736, 841
PsiI TTATAA 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 72, 180
Psp6I CCWGG 4 cut(s) 203, 649, 670, 1233
PspGI CCWGG 4 cut(s) 203, 649, 670, 1233
PspN4I GGNNCC 3 cut(s) 1769, 1818, 1989
PspPI GGNCC 3 cut(s) 1042, 1348, 1888
PstNI CAGNNNCTG 1 cut(s) 1454
PsuI RGATCY 3 cut(s) 411, 424, 781
PvuII CAGCTG 2 cut(s) 466, 1109
RsaI GTAC 3 cut(s) 290, 1076, 1375
RsaNI GTAC 3 cut(s) 289, 1075, 1374
RseI CAYNNNNRTG 1 cut(s) 1641
SacI GAGCTC 2 cut(s) 72, 180
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 225, 827
SatI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
Sau3AI GATC 9 cut(s) 111, 207, 211, 411, 424, 781, 1137, 1273, 1417
Sau96I GGNCC 3 cut(s) 1042, 1348, 1888
SchI GAGTC 3 cut(s) 386, 737, 841
ScrFI CCNGG 5 cut(s) 205, 651, 672, 1235, 1426
SduI GDGCHC 3 cut(s) 72, 180, 646
SfaNI GCATC 5 cut(s) 487, 826, 991, 2155, 2199
SfcI CTRYAG 2 cut(s) 1110, 1980
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 3 cut(s) 1042, 1348, 1888
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 1641
SmlI CTYRAG 3 cut(s) 428, 707, 1703
SmoI CTYRAG 3 cut(s) 428, 707, 1703
Sse9I AATT 7 cut(s) 158, 459, 1017, 1334, 1619, 1750, 1962
SsiI CCGC 9 cut(s) 479, 552, 609, 1004, 1045, 1214, 1510, 1814, 2183
SspMI CTAG 3 cut(s) 404, 614, 915
SstI GAGCTC 2 cut(s) 72, 180
StyD4I CCNGG 5 cut(s) 203, 649, 670, 1233, 1424
StyI CCWWGG 1 cut(s) 914
TaaI ACNGT 1 cut(s) 1028
TaiI ACGT 1 cut(s) 2123
TaqI TCGA 6 cut(s) 210, 429, 506, 694, 951, 1086
TasI AATT 7 cut(s) 158, 459, 1017, 1334, 1619, 1750, 1962
TatI WGTACW 1 cut(s) 1074
TauI GCSGC 1 cut(s) 482
Tru1I TTAA 2 cut(s) 225, 827
Tru9I TTAA 2 cut(s) 225, 827
TscAI CASTG 3 cut(s) 1068, 1485, 1678
TseFI GTSAC 1 cut(s) 1059
TseI GCWGC 6 cut(s) 791, 1115, 1163, 1454, 1604, 1691
Tsp45I GTSAC 1 cut(s) 1059
TspDTI ATGAA 7 cut(s) 70, 115, 171, 348, 465, 1104, 2505
TspRI CASTG 3 cut(s) 1068, 1485, 1678
VpaK11BI GGWCC 3 cut(s) 1042, 1348, 1888
XapI RAATTY 5 cut(s) 158, 459, 1334, 1750, 1962
XceI RCATGY 2 cut(s) 351, 1411
XcmI CCANNNNNNNNNTGG 2 cut(s) 1126, 1358
XhoI CTCGAG 1 cut(s) 428
XmaJI CCTAGG 1 cut(s) 914
XmiI GTMKAC 1 cut(s) 1341
XmnI GAANNNNTTC 2 cut(s) 1102, 1962
XspI CTAG 3 cut(s) 404, 614, 915
ZraI GACGTC 1 cut(s) 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.