FvH4_5g16010

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011994
Physical Location & Seq
Reverse (-)
9082944 .. 9086574
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16010.t1

Sequence Viewer

Length: 2091 bp
ATGAGTTCAAACAAGAAGACAATGGCAAAGGGACGCTTCACCCTATTTGTGGAGCAAAACATGGGACAGCTCCCACGTTTCATGGTCTATGCTGTGCTCGAATGGGTCTTGATCATTCTGCTCTTCCTTGATGGCTTTGTTGCGTTTCTTGCCAATGAGTTTGCCAAGTTATTTGAGCTCACAACTCCCTGCTGGCTTTGCACCAAGATCGATCACATTCTCGTTAACAGAGGTCGTGATTTCTATTACAATGATTCCATATGTGAATCTCACAAGAAAGATGTTTCCTACCTTGCTTACTGCCACAACCACAAGAGGCTATCTGACATAAGAAAGATGTGTGAGGCATGTCTTCTTTCATTTGCAACCGAGAAAGAGTCTGATTTCGACACATACAAGTCCCTAGTTGGGATCCTGCACAAGGATCTTGAGTGCTTTGTGGAGGATGATCAACATCAAATTCAGCTGAGTGTGCCTGCAGCAAGGACATGGGAGGATGCAGGGACCATTGAGAAGAGTAGGGACAGCATGACTATAGGGTGTTCGTGTTGCGGTGCGCCATTGAAGTTGAGTCAGTCTCCCTCCTACCCCAAAGGGAAAAATGTAACCGCGCTTGCGCAGGCTCCTACACCTTCTCCACGAGCACCCTTAGGGAAAAATGATGATGCTCCTGGTTTGGACTTGCCCCCTATTCGATACTCAGAACTCAAGTTTATGTCTGAAAATGAGTCCGAGCTTCCAGAGGATGAGTATGGAGCACACTCAAAAAATCAAAAGGAAGATCCGAAAGCTGCTACACTGCCACTATTGGCAGAAGGTGATGAATTAACTGATGATGCTAATAGGACTCCCCTATTTGGTAGAGGAAACAAGTTCTTTGGAATACCACTAACAGATTCAGCTCAAAATAGTCCTAGGGTGGCTATGAGGATCTCAAAGAAATCACCACTTGAAAAGAGCGAGTTTGTTTTGGAATCGGTTGATGGAAATCTTCCAAATGATACGGATTGTGATTCAATTTTGCAACGTCTGAAGAGACAGGTTCGCTTGGACAAGAAGTCCCTAATGGCTCTGTACATGGAACTTGACGAAGAAAGAAGTGCTTCAGCTGTAGCAGCTGACGAGGCAATGGCTATGATCACAAGGCTACAAGCAGAGAAGGCTGCTGTTCAGATGGAGGCATTGCAGTATCAGAGAATGATGGAGGAGCAGGCGGAGTATGACCAAGAAGCCCTGGAAGCAACATATGACTTGCTTGCCAAGAGAGAAGATGATATGAGGGTTTTAGAGGCTGAAATAGAGGCTTATAGGGAAAAATATGGATTATTGAAGGAATTTGTCTACAAGGACCAAGATGAGGCTGCTGAATCTGGTAATTCTTCAAGCGCAAACAACGCGCAAAATGATCAAGCTGGGTCAGCAAAGGAGGAGAATGCAGTCGACACTGCCCTTGCTGAAAGTTTGAAACCACTGAAAGGGGAGAAGACCTACCTCCTTGGGCGGCTCAAGAAACTAGACAAGAGAAACTTATCAAACTTGTCATCTAATGGGTTTTCCTTGCCGGATGGTCTGAATGAGGATGAGATAGGAAATGGAAACAAAGCTGCCCTAACAAGACAATTGTCCCTATTGTCCCATCTTAGTGATAGGGTGAAGGCTCTTGAATCAGACACTGGATTCCTAGAGTATGCTGCTAAGTCACTTGAGAAACATAGTGAAGGAACAAAACTTTTGACAGAGATAACTCAAAATTTACAGAAACTCCGGCACCTTGTGAATCTGCCTACACTGCCTACACCACCTACAGAGGAACAAAACAAGATGGCTCCGGAGGAACACAGGAAGGAGCAAAACAAGACACCTTTAGAGGACCAAAAGAAGGAGCAAACTAAGACGCCTACAGAGGAGCCTCTAGAAGAACAAAAGAAGGAGCAAAGCGAGCTAAACAAGACGCATCCGGAGGAACAAAAGCAGTCTGTTTTTGCTCGAATGTTTTGGGGCCAAACACAGTCATCGGAGGAACAAAAGCAGTCATCTCCGGAAGAACAAAACAAGACGTCTTCAGAGGCACAAAAAGATGGACTACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

697

Amino Acids

78.39

Weight (kDa)

5.07

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 343 - 433 1.5e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2060
Acc16I TGCGCA 1 cut(s) 618
AccB1I GGYRCC 1 cut(s) 1767
AccI GTMKAC 2 cut(s) 1341, 1440
AccII CGCG 2 cut(s) 611, 1397
AccIII TCCGGA 3 cut(s) 1828, 1957, 2038
AciI CCGC 4 cut(s) 552, 609, 1214, 1501
AclWI GGATC 5 cut(s) 406, 419, 432, 776, 938
AcsI RAATTY 3 cut(s) 459, 1334, 1750
AcuI CTGAAG 3 cut(s) 1052, 1089, 2046
AcyI GRCGYC 2 cut(s) 1895, 2057
AdeI CACNNNGTG 1 cut(s) 1774
AfaI GTAC 1 cut(s) 1076
AfiI CCNNNNNNNGG 7 cut(s) 49, 408, 421, 857, 1357, 1475, 1879
AgsI TTSAA 8 cut(s) 9, 565, 953, 1017, 1330, 1383, 1465, 1664
AhdI GACNNNNNGTC 1 cut(s) 1057
AjnI CCWGG 2 cut(s) 670, 1233
Alw21I GWGCWC 4 cut(s) 99, 180, 646, 760
Alw26I GTCTC 2 cut(s) 582, 1030
AlwI GGATC 5 cut(s) 406, 419, 432, 776, 938
AlwNI CAGNNNCTG 1 cut(s) 1673
Aor13HI TCCGGA 3 cut(s) 1828, 1957, 2038
AoxI GGCC 1 cut(s) 1999
ApeKI GCWGC 7 cut(s) 479, 791, 1115, 1163, 1361, 1604, 1691
ApoI RAATTY 3 cut(s) 459, 1334, 1750
Asp700I GAANNNNTTC 1 cut(s) 1102
AspA2I CCTAGG 1 cut(s) 914
AspLEI GCGC 5 cut(s) 559, 613, 619, 1388, 1399
AspS9I GGNCC 4 cut(s) 504, 1348, 1870, 1999
AsuHPI GGTGA 4 cut(s) 31, 830, 936, 1663
AvaII GGWCC 3 cut(s) 504, 1348, 1870
AvrII CCTAGG 1 cut(s) 914
AxyI CCTNAGG 1 cut(s) 649
BamHI GGATCC 1 cut(s) 411
BanI GGYRCC 1 cut(s) 1767
BanII GRGCYC 1 cut(s) 180
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 4 cut(s) 23, 344, 1490, 2052
Bbv12I GWGCWC 4 cut(s) 99, 180, 646, 760
BbvI GCAGC 7 cut(s) 491, 778, 1127, 1150, 1348, 1591, 1678
BccI CCATC 8 cut(s) 125, 977, 1168, 1195, 1559, 1644, 1816, 2072
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 2 cut(s) 672, 1235
BclI TGATCA 4 cut(s) 111, 448, 1137, 1405
BcoDI GTCTC 2 cut(s) 582, 1030
BfaI CTAG 5 cut(s) 404, 915, 1514, 1682, 1913
BfmI CTRYAG 5 cut(s) 477, 534, 1110, 1803, 1899
BisI GCNGC 8 cut(s) 480, 792, 1116, 1164, 1362, 1502, 1605, 1692
BlnI CCTAGG 1 cut(s) 914
BlsI GCNGC 8 cut(s) 481, 793, 1117, 1165, 1363, 1503, 1606, 1693
Bme1390I CCNGG 2 cut(s) 672, 1235
Bme18I GGWCC 3 cut(s) 504, 1348, 1870
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmgT120I GGNCC 4 cut(s) 504, 1348, 1870, 1999
BmiI GGNNCC 7 cut(s) 413, 505, 624, 1769, 1827, 1908, 2000
BmrFI CCNGG 2 cut(s) 672, 1235
BmsI GCATC 4 cut(s) 487, 655, 826, 1963
BoxI GACNNNNGTC 1 cut(s) 1621
BpiI GAAGAC 4 cut(s) 23, 344, 1490, 2052
BplI GAGNNNNNCTC 2 cut(s) 562, 594
BpuEI CTTGAG 4 cut(s) 449, 692, 1490, 1724
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 2 cut(s) 453, 987
BsaHI GRCGYC 2 cut(s) 1895, 2057
BsaJI CCNNGG 3 cut(s) 914, 1233, 1495
BsaWI WCCGGW 3 cut(s) 1828, 1957, 2038
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 1746, 1776
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 408, 421, 857, 1357, 1475, 1879
Bse1I ACTGG 1 cut(s) 1678
Bse21I CCTNAGG 1 cut(s) 649
Bse3DI GCAATG 2 cut(s) 1134, 1181
Bse8I GATNNNNATC 2 cut(s) 453, 987
BseAI TCCGGA 3 cut(s) 1828, 1957, 2038
BseBI CCWGG 2 cut(s) 672, 1235
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 914, 1233, 1495
BseGI GGATG 6 cut(s) 451, 502, 751, 1570, 1585, 1954
BseJI GATNNNNATC 2 cut(s) 453, 987
BseLI CCNNNNNNNGG 7 cut(s) 49, 408, 421, 857, 1357, 1475, 1879
BseMI GCAATG 2 cut(s) 1134, 1181
BseMII CTCAG 2 cut(s) 458, 714
BseNI ACTGG 1 cut(s) 1678
BseRI GAGGAG 3 cut(s) 1220, 1442, 1919
BseXI GCAGC 7 cut(s) 491, 778, 1127, 1150, 1348, 1591, 1678
BseYI CCCAGC 1 cut(s) 1412
BsgI GTGCAG 1 cut(s) 401
Bsh1236I CGCG 2 cut(s) 611, 1397
BshFI GGCC 1 cut(s) 2001
BshNI GGYRCC 1 cut(s) 1767
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 4 cut(s) 99, 180, 646, 760
BsiSI CCGG 5 cut(s) 1562, 1765, 1829, 1958, 2039
BslFI GGGAC 8 cut(s) 45, 78, 385, 517, 536, 1045, 1609, 1618
BslI CCNNNNNNNGG 7 cut(s) 49, 408, 421, 857, 1357, 1475, 1879
BsmAI GTCTC 2 cut(s) 582, 1030
BsmFI GGGAC 8 cut(s) 45, 78, 385, 517, 536, 1045, 1609, 1618
BsmI GAATGC 1 cut(s) 1438
BsnI GGCC 1 cut(s) 2001
Bsp1286I GDGCHC 4 cut(s) 99, 180, 646, 760
Bsp13I TCCGGA 3 cut(s) 1828, 1957, 2038
Bsp1407I TGTACA 1 cut(s) 1074
BspACI CCGC 4 cut(s) 552, 609, 1214, 1501
BspANI GGCC 1 cut(s) 2001
BspCNI CTCAG 2 cut(s) 459, 713
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 3 cut(s) 1828, 1957, 2038
BspFNI CGCG 2 cut(s) 611, 1397
BspLI GGNNCC 7 cut(s) 413, 505, 624, 1769, 1827, 1908, 2000
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 5 cut(s) 406, 419, 432, 776, 938
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 1767
BsrDI GCAATG 2 cut(s) 1134, 1181
BsrGI TGTACA 1 cut(s) 1074
BsrI ACTGG 1 cut(s) 1678
BssECI CCNNGG 3 cut(s) 914, 1233, 1495
BssNI GRCGYC 2 cut(s) 1895, 2057
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 2 cut(s) 914, 1495
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 2 cut(s) 672, 1235
Bst4CI ACNGT 1 cut(s) 2010
Bst6I CTCTTC 3 cut(s) 128, 509, 1028
BstACI GRCGYC 2 cut(s) 1895, 2057
BstAUI TGTACA 1 cut(s) 1074
BstC8I GCNNGC 7 cut(s) 194, 477, 615, 621, 1212, 1257, 1940
BstDEI CTNAG 7 cut(s) 467, 649, 700, 1640, 1695, 1890, 2088
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 6 cut(s) 451, 502, 751, 1570, 1585, 1954
BstFNI CGCG 2 cut(s) 611, 1397
BstHHI GCGC 5 cut(s) 559, 613, 619, 1388, 1399
BstMAI GTCTC 2 cut(s) 582, 1030
BstNI CCWGG 2 cut(s) 672, 1235
BstNSI RCATGY 1 cut(s) 351
BstPAI GACNNNNGTC 1 cut(s) 1621
BstSCI CCNGG 2 cut(s) 670, 1233
BstSFI CTRYAG 5 cut(s) 477, 534, 1110, 1803, 1899
BstUI CGCG 2 cut(s) 611, 1397
BstV1I GCAGC 7 cut(s) 491, 778, 1127, 1150, 1348, 1591, 1678
BstV2I GAAGAC 4 cut(s) 23, 344, 1490, 2052
BstX2I RGATCY 4 cut(s) 411, 424, 781, 930
BstYI RGATCY 4 cut(s) 411, 424, 781, 930
Bsu15I ATCGAT 1 cut(s) 210
Bsu36I CCTNAGG 1 cut(s) 649
BsuRI GGCC 1 cut(s) 2001
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 6 cut(s) 451, 502, 751, 1570, 1585, 1954
BtsI GCAGTG 3 cut(s) 797, 1443, 1787
BtsIMutI CAGTG 5 cut(s) 797, 1443, 1469, 1671, 1787
Cac8I GCNNGC 7 cut(s) 194, 477, 615, 621, 1212, 1257, 1940
CaiI CAGNNNCTG 1 cut(s) 1673
CfoI GCGC 5 cut(s) 559, 613, 619, 1388, 1399
Cfr13I GGNCC 4 cut(s) 504, 1348, 1870, 1999
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 3 cut(s) 42, 1903, 1960
Csp6I GTAC 1 cut(s) 1075
CviAII CATG 6 cut(s) 61, 82, 348, 489, 529, 1078
CviQI GTAC 1 cut(s) 1075
DdeI CTNAG 7 cut(s) 467, 649, 700, 1640, 1695, 1890, 2088
DraIII CACNNNGTG 1 cut(s) 1774
DriI GACNNNNNGTC 1 cut(s) 1057
Eam1104I CTCTTC 3 cut(s) 128, 509, 1028
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 3 cut(s) 128, 509, 1028
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 914, 1495
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 3 cut(s) 504, 1348, 1870
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 3 cut(s) 1052, 1089, 2046
Eco81I CCTNAGG 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 178
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 670, 1233
EcoT14I CCWWGG 2 cut(s) 914, 1495
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 914, 1495
FaeI CATG 6 cut(s) 64, 85, 351, 492, 532, 1081
FalI AAGNNNNNCTT 6 cut(s) 20, 52, 1087, 1119, 1511, 1543
FaqI GGGAC 8 cut(s) 45, 78, 385, 517, 536, 1045, 1609, 1618
FatI CATG 6 cut(s) 60, 81, 347, 488, 528, 1077
FauNDI CATATG 2 cut(s) 260, 1246
FbaI TGATCA 4 cut(s) 111, 448, 1137, 1405
FblI GTMKAC 2 cut(s) 1341, 1440
Fnu4HI GCNGC 8 cut(s) 480, 792, 1116, 1164, 1362, 1502, 1605, 1692
FokI GGATG 6 cut(s) 458, 509, 758, 1577, 1592, 1941
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 8 cut(s) 480, 792, 1116, 1164, 1362, 1502, 1605, 1692
FspBI CTAG 5 cut(s) 404, 915, 1514, 1682, 1913
FspI TGCGCA 1 cut(s) 618
GlaI GCGC 5 cut(s) 558, 612, 618, 1387, 1398
GluI GCNGC 8 cut(s) 480, 792, 1116, 1164, 1362, 1502, 1605, 1692
GsaI CCCAGC 1 cut(s) 1416
HaeIII GGCC 1 cut(s) 2001
HapII CCGG 5 cut(s) 1562, 1765, 1829, 1958, 2039
HgaI GACGC 3 cut(s) 42, 1903, 1960
HhaI GCGC 5 cut(s) 559, 613, 619, 1388, 1399
Hin1I GRCGYC 2 cut(s) 1895, 2057
Hin1II CATG 6 cut(s) 64, 85, 351, 492, 532, 1081
Hin6I GCGC 5 cut(s) 557, 611, 617, 1386, 1397
HinP1I GCGC 5 cut(s) 557, 611, 617, 1386, 1397
HincII GTYRAC 2 cut(s) 226, 1441
HindII GTYRAC 2 cut(s) 226, 1441
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 5 cut(s) 1562, 1765, 1829, 1958, 2039
HphI GGTGA 4 cut(s) 31, 830, 936, 1663
Hpy166II GTNNAC 3 cut(s) 226, 1342, 1441
Hpy8I GTNNAC 3 cut(s) 226, 1342, 1441
HpyAV CCTTC 9 cut(s) 642, 809, 1153, 1324, 1648, 1712, 1837, 1873, 1921
HpyCH4III ACNGT 1 cut(s) 2010
HpyCH4IV ACGT 3 cut(s) 76, 1027, 2057
HpyCH4V TGCA 8 cut(s) 201, 365, 418, 479, 500, 1024, 1186, 1436
HpyF3I CTNAG 7 cut(s) 467, 649, 700, 1640, 1695, 1890, 2088
HpySE526I ACGT 3 cut(s) 76, 1027, 2057
Hsp92I GRCGYC 2 cut(s) 1895, 2057
Hsp92II CATG 6 cut(s) 64, 85, 351, 492, 532, 1081
HspAI GCGC 5 cut(s) 557, 611, 617, 1386, 1397
Kpn2I TCCGGA 3 cut(s) 1828, 1957, 2038
Ksp22I TGATCA 4 cut(s) 111, 448, 1137, 1405
KspAI GTTAAC 1 cut(s) 226
LguI GCTCTTC 1 cut(s) 128
Lsp1109I GCAGC 7 cut(s) 491, 778, 1127, 1150, 1348, 1591, 1678
LweI GCATC 4 cut(s) 487, 655, 826, 1963
MaeI CTAG 5 cut(s) 404, 915, 1514, 1682, 1913
MaeII ACGT 3 cut(s) 76, 1027, 2057
MaeIII GTNAC 2 cut(s) 604, 1698
MfeI CAATTG 1 cut(s) 1619
MflI RGATCY 4 cut(s) 411, 424, 781, 930
MhlI GDGCHC 4 cut(s) 99, 180, 646, 760
MluCI AATT 7 cut(s) 459, 824, 1017, 1334, 1375, 1619, 1750
MlyI GAGTC 4 cut(s) 386, 580, 737, 841
MroI TCCGGA 3 cut(s) 1828, 1957, 2038
MroXI GAANNNNTTC 1 cut(s) 1102
MseI TTAA 2 cut(s) 225, 827
MslI CAYNNNNRTG 1 cut(s) 1641
MspA1I CMGCKG 3 cut(s) 466, 1109, 1118
MspI CCGG 5 cut(s) 1562, 1765, 1829, 1958, 2039
MspR9I CCNGG 2 cut(s) 672, 1235
MunI CAATTG 1 cut(s) 1619
Mva1269I GAATGC 1 cut(s) 1438
MvaI CCWGG 2 cut(s) 672, 1235
MvnI CGCG 2 cut(s) 611, 1397
NdeI CATATG 2 cut(s) 260, 1246
NlaIII CATG 6 cut(s) 64, 85, 351, 492, 532, 1081
NlaIV GGNNCC 7 cut(s) 413, 505, 624, 1769, 1827, 1908, 2000
NmuCI GTSAC 1 cut(s) 1698
NsbI TGCGCA 1 cut(s) 618
NspI RCATGY 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 128
PctI GAATGC 1 cut(s) 1438
PdmI GAANNNNTTC 1 cut(s) 1102
PfeI GAWTC 9 cut(s) 254, 266, 896, 974, 1013, 1367, 1664, 1677, 1777
PkrI GCNGC 8 cut(s) 481, 793, 1117, 1165, 1363, 1503, 1606, 1693
PleI GAGTC 4 cut(s) 385, 579, 736, 841
PpsI GAGTC 4 cut(s) 385, 579, 736, 841
PshAI GACNNNNGTC 1 cut(s) 1621
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 670, 1233
PspFI CCCAGC 1 cut(s) 1412
PspGI CCWGG 2 cut(s) 670, 1233
PspN4I GGNNCC 7 cut(s) 413, 505, 624, 1769, 1827, 1908, 2000
PspPI GGNCC 4 cut(s) 504, 1348, 1870, 1999
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 1673
PsuI RGATCY 4 cut(s) 411, 424, 781, 930
PvuII CAGCTG 3 cut(s) 466, 1109, 1118
RsaI GTAC 1 cut(s) 1076
RsaNI GTAC 1 cut(s) 1075
RseI CAYNNNNRTG 1 cut(s) 1641
SacI GAGCTC 1 cut(s) 180
SalI GTCGAC 1 cut(s) 1439
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 225, 827
SatI GCNGC 8 cut(s) 480, 792, 1116, 1164, 1362, 1502, 1605, 1692
Sau96I GGNCC 4 cut(s) 504, 1348, 1870, 1999
SchI GAGTC 4 cut(s) 386, 580, 737, 841
ScrFI CCNGG 2 cut(s) 672, 1235
SduI GDGCHC 4 cut(s) 99, 180, 646, 760
SfaNI GCATC 4 cut(s) 487, 655, 826, 1963
SfcI CTRYAG 5 cut(s) 477, 534, 1110, 1803, 1899
SinI GGWCC 3 cut(s) 504, 1348, 1870
SmiMI CAYNNNNRTG 1 cut(s) 1641
SmlI CTYRAG 4 cut(s) 428, 707, 1505, 1703
SmoI CTYRAG 4 cut(s) 428, 707, 1505, 1703
Sse9I AATT 7 cut(s) 459, 824, 1017, 1334, 1375, 1619, 1750
SsiI CCGC 4 cut(s) 552, 609, 1214, 1501
SspMI CTAG 5 cut(s) 404, 915, 1514, 1682, 1913
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 670, 1233
StyI CCWWGG 2 cut(s) 914, 1495
TaaI ACNGT 1 cut(s) 2010
TaiI ACGT 3 cut(s) 79, 1030, 2060
TaqI TCGA 6 cut(s) 99, 210, 387, 694, 1440, 1987
TasI AATT 7 cut(s) 459, 824, 1017, 1334, 1375, 1619, 1750
TatI WGTACW 1 cut(s) 1074
TauI GCSGC 1 cut(s) 1504
TfiI GAWTC 9 cut(s) 254, 266, 896, 974, 1013, 1367, 1664, 1677, 1777
Tru1I TTAA 2 cut(s) 225, 827
Tru9I TTAA 2 cut(s) 225, 827
TscAI CASTG 5 cut(s) 804, 1450, 1476, 1678, 1794
TseFI GTSAC 1 cut(s) 1698
TseI GCWGC 7 cut(s) 479, 791, 1115, 1163, 1361, 1604, 1691
Tsp45I GTSAC 1 cut(s) 1698
TspDTI ATGAA 3 cut(s) 70, 348, 837
TspGWI ACGGA 1 cut(s) 1019
TspRI CASTG 5 cut(s) 804, 1450, 1476, 1678, 1794
VpaK11BI GGWCC 3 cut(s) 504, 1348, 1870
XagI CCTNNNNNAGG 1 cut(s) 419
XapI RAATTY 3 cut(s) 459, 1334, 1750
XbaI TCTAGA 1 cut(s) 1912
XceI RCATGY 1 cut(s) 351
XmaJI CCTAGG 1 cut(s) 914
XmiI GTMKAC 2 cut(s) 1341, 1440
XmnI GAANNNNTTC 1 cut(s) 1102
XspI CTAG 5 cut(s) 404, 915, 1514, 1682, 1913
ZraI GACGTC 1 cut(s) 2058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.