AT1G74830

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011994
Physical Location & Seq
Reverse (-)
28113194 .. 28115281
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74830.1

Sequence Viewer

Length: 1629 bp
ATGTATATACAATTGCTCTGTTTTTTCTTGTTCTTGTTTCTGCTTTTACAAGCCACAATGTCTAAACGTTCTTTCAAGAAATTTGTGGAACAAGAACTCGGATCTCTTCCTCATTTCCTCATCTACACTGTTCTTGAATGGAGCCTCATTGTTTTCCTCTTCATCGACGGCGTTATCGCCTTTCTGTCAAACCAATTCGCCAAATTCTTCGACCTCAACATTCCTTGTCTTCTCTGCACCAGAATCGACCACATTCTCGTCCCTAGAGACCCTCAATTCTATTACAACGAGTCAATCTGCGACTCTCACAAGAAGAAAGTATCTTCTCTTGCTTATTGCCATGTCCATAAGAAGCTCTCTGAGATTAAACATATGTGTGAAGGATGTCTTCTCTCTTTCGCCACCGAGAAAGATTCTGACTGTGATACTTATAAGTCTCTCATTGGCATCTTACATAAAGATCTCGAGCTTCTCATTGACGATGAACGAGACCTTCCCTTGGCGTTCAAGAAAGACGACAATTTAGTCCAGACAACAAAAAATCTGGTGGATTACAAGACTAACAACATTAAAAATGATAGCTTGAAGCAACATTGTTCGTGTTGTGGAGAATTATTGAAGATAAAATCTGAGAAGTTACCGAAGAACAACAATTCTTTCCTTGCTCCTGCTCCTTCTCCTAGGGTATCGCACAACAAACTGTCTGAAAACGAATCTGAGTTTAAGGATATGGATGTGGACCGAACACCAAGTTTTGTAAGAGGAGGTAACAAGTTTTTTGGGATCCCTCTGTCTGATTCGGCGCAAAACAGTCCACGATGGTCCGTTCGATCTCTTAAGAAATCCGTTTTGAATAAGACAGAGAATGCTTCGGACACGACAGATCCAACCGGTGAATCCATTCTTAATCAGCTCAAGAAAGAGGTTCGCTTGGACAAGAAGTCACTCATTGATTTATATATGGAGTTAGATGAAGAGAGAAGTGCTTCAGCTGTTGCAGCCAACGAAGCGATGGCTATGATCACGAGGCTTCAAGCCGAGAAAGCTGCTGTTCAAATGGAGGCTTTGCAGTACCAAAGAATGATGGATGAACAAGCAGAGTATGACCAAGAAGCTTTGCAGTCTATGAGTAGTGAATTGGCGAAGAGGGAAGAGGAAATGAAGGAGCTTGAAGCTGAATTTGAGGTTTACAGAGAGAAATATGGATGTTTGACTGATCAAGAAGATGCAAGGGAAGAGTTTCATAAACAAAACGGCAATGCTAGTGCATATGATGATTGCCAAGAAACCAAACCAGTCTCGGATTTGGCTGTCTCTTCTTCAAATCAGCAGGAAAATGGCGAAAACATCGATCAAAATGGGCAATCGAAAAGGTCAGAGGAGTCTACCGCGGAGAATGTTGTCTCTGCAGATGAAGAAAAAGGGAGTGAAAGCAAAGAAGGGATAGTAAAAGAGCTTTCAGAGATCACAGAGAGACTAAGTACTCTTCAATCAAATGGTGATTTATTGAAACACATTGCTGATGTTTTGGATGTTAGTGAAGGAGAAGCAATTCTATTGCAGATATCTCAGAATTTGCACATGCTTCGTAGTTTTGTTGCTATGCCTTCTGAATCCATGAACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

542

Amino Acids

61.61

Weight (kDa)

4.95

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 304 - 393 9.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AasI GACNNNNNNGTC 1 cut(s) 524
AccI GTMKAC 1 cut(s) 1385
AccII CGCG 1 cut(s) 1391
AciI CCGC 2 cut(s) 1389, 1391
AclI AACGTT 1 cut(s) 67
AclWI GGATC 4 cut(s) 109, 778, 791, 878
AcsI RAATTY 4 cut(s) 80, 203, 1178, 1573
AcuI CTGAAG 1 cut(s) 972
AfaI GTAC 2 cut(s) 1073, 1483
AfiI CCNNNNNNNGG 1 cut(s) 499
AflII CTTAAG 1 cut(s) 836
AgeI ACCGGT 1 cut(s) 890
AhdI GACNNNNNGTC 1 cut(s) 940
Alw26I GTCTC 7 cut(s) 261, 441, 483, 1303, 1318, 1408, 1468
AlwI GGATC 4 cut(s) 109, 778, 791, 878
Ama87I CYCGRG 1 cut(s) 464
ApeKI GCWGC 2 cut(s) 998, 1046
ApoI RAATTY 4 cut(s) 80, 203, 1178, 1573
AsiGI ACCGGT 1 cut(s) 890
Asp700I GAANNNNTTC 4 cut(s) 900, 985, 1239, 1551
AspA2I CCTAGG 1 cut(s) 680
AspLEI GCGC 1 cut(s) 805
AspS9I GGNCC 2 cut(s) 739, 822
AsuHPI GGTGA 2 cut(s) 905, 1511
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 2 cut(s) 739, 822
AvrII CCTAGG 1 cut(s) 680
BamHI GGATCC 1 cut(s) 783
BauI CACGAG 1 cut(s) 1024
BbsI GAAGAC 2 cut(s) 221, 380
BbvI GCAGC 2 cut(s) 1010, 1033
BccI CCATC 3 cut(s) 813, 1006, 1078
BceAI ACGGC 2 cut(s) 184, 1270
BcgI CGANNNNNNTGC 6 cut(s) 226, 260, 1028, 1062, 1568, 1602
BclI TGATCA 2 cut(s) 1020, 1216
BcoDI GTCTC 7 cut(s) 261, 441, 483, 1303, 1318, 1408, 1468
BfaI CTAG 3 cut(s) 264, 681, 1263
BfmI CTRYAG 1 cut(s) 1407
BfrI CTTAAG 1 cut(s) 836
BglII AGATCT 1 cut(s) 460
BisI GCNGC 2 cut(s) 999, 1047
BlnI CCTAGG 1 cut(s) 680
BlsI GCNGC 2 cut(s) 1000, 1048
BmcAI AGTACT 1 cut(s) 1483
Bme18I GGWCC 2 cut(s) 739, 822
BmeRI GACNNNNNGTC 1 cut(s) 940
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 739, 822
BmiI GGNNCC 2 cut(s) 143, 785
BmsI GCATC 2 cut(s) 456, 1216
BpiI GAAGAC 2 cut(s) 221, 380
BpuEI CTTGAG 1 cut(s) 899
Bsa29I ATCGAT 1 cut(s) 1350
BsaI GGTCTC 2 cut(s) 261, 483
BsaJI CCNNGG 3 cut(s) 498, 680, 1389
BsaWI WCCGGW 1 cut(s) 890
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse118I RCCGGY 1 cut(s) 890
Bse1I ACTGG 1 cut(s) 1295
Bse3DI GCAATG 2 cut(s) 1264, 1515
BseCI ATCGAT 1 cut(s) 1350
BseDI CCNNGG 3 cut(s) 498, 680, 1389
BseGI GGATG 5 cut(s) 389, 739, 1093, 1211, 1537
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMI GCAATG 2 cut(s) 1264, 1515
BseMII CTCAG 4 cut(s) 351, 621, 708, 1583
BseNI ACTGG 1 cut(s) 1295
BseRI GAGGAG 2 cut(s) 777, 1394
BseXI GCAGC 2 cut(s) 1010, 1033
BsgI GTGCAG 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 1391
BshTI ACCGGT 1 cut(s) 890
BshVI ATCGAT 1 cut(s) 1350
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 1 cut(s) 891
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 7 cut(s) 261, 441, 483, 1303, 1318, 1408, 1468
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 871
Bso31I GGTCTC 2 cut(s) 261, 483
BsoBI CYCGRG 1 cut(s) 464
Bsp143I GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
BspACI CCGC 2 cut(s) 1389, 1391
BspCNI CTCAG 4 cut(s) 352, 622, 709, 1582
BspDI ATCGAT 1 cut(s) 1350
BspFNI CGCG 1 cut(s) 1391
BspLI GGNNCC 2 cut(s) 143, 785
BspMAI CTGCAG 1 cut(s) 1411
BspPI GGATC 4 cut(s) 109, 778, 791, 878
BspTI CTTAAG 1 cut(s) 836
BspTNI GGTCTC 2 cut(s) 261, 483
BsrDI GCAATG 2 cut(s) 1264, 1515
BsrFI RCCGGY 1 cut(s) 890
BsrI ACTGG 1 cut(s) 1295
BssAI RCCGGY 1 cut(s) 890
BssECI CCNNGG 3 cut(s) 498, 680, 1389
BssMI GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
BssSI CACGAG 1 cut(s) 1024
BssT1I CCWWGG 2 cut(s) 498, 680
Bst2BI CACGAG 1 cut(s) 1024
Bst4CI ACNGT 4 cut(s) 130, 422, 702, 812
Bst6I CTCTTC 8 cut(s) 111, 164, 969, 1139, 1146, 1230, 1321, 1491
BstAFI CTTAAG 1 cut(s) 836
BstDEI CTNAG 5 cut(s) 360, 630, 717, 1478, 1569
BstDSI CCRYGG 1 cut(s) 1389
BstF5I GGATG 5 cut(s) 389, 739, 1093, 1211, 1537
BstFNI CGCG 1 cut(s) 1391
BstHHI GCGC 1 cut(s) 805
BstKTI GATC 9 cut(s) 104, 463, 786, 833, 886, 1023, 1219, 1354, 1467
BstMAI GTCTC 7 cut(s) 261, 441, 483, 1303, 1318, 1408, 1468
BstMBI GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
BstMWI GCNNNNNNNGC 3 cut(s) 998, 1007, 1043
BstNSI RCATGY 1 cut(s) 1585
BstSFI CTRYAG 1 cut(s) 1407
BstUI CGCG 1 cut(s) 1391
BstV1I GCAGC 2 cut(s) 1010, 1033
BstV2I GAAGAC 2 cut(s) 221, 380
BstX2I RGATCY 4 cut(s) 101, 460, 783, 883
BstYI RGATCY 4 cut(s) 101, 460, 783, 883
Bsu15I ATCGAT 1 cut(s) 1350
BsuTUI ATCGAT 1 cut(s) 1350
BtgI CCRYGG 1 cut(s) 1389
BtgZI GCGATG 1 cut(s) 1025
BtsCI GGATG 5 cut(s) 389, 739, 1093, 1211, 1537
BtsIMutI CAGTG 1 cut(s) 126
CfoI GCGC 1 cut(s) 805
Cfr10I RCCGGY 1 cut(s) 890
Cfr13I GGNCC 2 cut(s) 739, 822
Cfr42I CCGCGG 1 cut(s) 1392
ClaI ATCGAT 1 cut(s) 1350
Csp6I GTAC 2 cut(s) 1072, 1482
CspAI ACCGGT 1 cut(s) 890
CviAII CATG 3 cut(s) 341, 1582, 1618
CviQI GTAC 2 cut(s) 1072, 1482
DdeI CTNAG 5 cut(s) 360, 630, 717, 1478, 1569
DpnI GATC 9 cut(s) 103, 462, 785, 832, 885, 1022, 1218, 1353, 1466
DpnII GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
DrdI GACNNNNNNGTC 1 cut(s) 524
DriI GACNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 1 cut(s) 524
Eam1104I CTCTTC 8 cut(s) 111, 164, 969, 1139, 1146, 1230, 1321, 1491
Eam1105I GACNNNNNGTC 1 cut(s) 940
EarI CTCTTC 8 cut(s) 111, 164, 969, 1139, 1146, 1230, 1321, 1491
Eco130I CCWWGG 2 cut(s) 498, 680
Eco31I GGTCTC 2 cut(s) 261, 483
Eco32I GATATC 1 cut(s) 1566
Eco47I GGWCC 2 cut(s) 739, 822
Eco57I CTGAAG 1 cut(s) 972
Eco88I CYCGRG 1 cut(s) 464
EcoRV GATATC 1 cut(s) 1566
EcoT14I CCWWGG 2 cut(s) 498, 680
ErhI CCWWGG 2 cut(s) 498, 680
FaeI CATG 3 cut(s) 344, 1585, 1621
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 1 cut(s) 245
FatI CATG 3 cut(s) 340, 1581, 1617
FauNDI CATATG 2 cut(s) 372, 1270
FbaI TGATCA 2 cut(s) 1020, 1216
FblI GTMKAC 1 cut(s) 1385
Fnu4HI GCNGC 2 cut(s) 999, 1047
FokI GGATG 5 cut(s) 396, 746, 1100, 1218, 1544
Fsp4HI GCNGC 2 cut(s) 999, 1047
FspBI CTAG 3 cut(s) 264, 681, 1263
GlaI GCGC 1 cut(s) 804
GluI GCNGC 2 cut(s) 999, 1047
HapII CCGG 1 cut(s) 891
HhaI GCGC 1 cut(s) 805
Hin1II CATG 3 cut(s) 344, 1585, 1621
Hin6I GCGC 1 cut(s) 803
HinP1I GCGC 1 cut(s) 803
HindIII AAGCTT 1 cut(s) 1113
HinfI GANTC 9 cut(s) 243, 290, 302, 413, 713, 797, 896, 1382, 1613
HpaII CCGG 1 cut(s) 891
HphI GGTGA 2 cut(s) 905, 1511
Hpy166II GTNNAC 4 cut(s) 739, 815, 1189, 1386
Hpy188III TCNNGA 8 cut(s) 76, 134, 464, 508, 529, 916, 1024, 1220
Hpy8I GTNNAC 4 cut(s) 739, 815, 1189, 1386
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 7 cut(s) 374, 503, 684, 1156, 1433, 1535, 1617
HpyCH4III ACNGT 4 cut(s) 130, 422, 702, 812
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 9 cut(s) 237, 998, 1069, 1120, 1229, 1268, 1409, 1561, 1579
HpyF10VI GCNNNNNNNGC 3 cut(s) 998, 1007, 1043
HpyF3I CTNAG 5 cut(s) 360, 630, 717, 1478, 1569
HpySE526I ACGT 1 cut(s) 67
Hsp92II CATG 3 cut(s) 344, 1585, 1621
HspAI GCGC 1 cut(s) 803
Ksp22I TGATCA 2 cut(s) 1020, 1216
KspI CCGCGG 1 cut(s) 1392
Kzo9I GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
LmnI GCTCC 4 cut(s) 141, 670, 676, 1165
LpnPI CCDG 7 cut(s) 253, 530, 542, 681, 904, 1308, 1316
Lsp1109I GCAGC 2 cut(s) 1010, 1033
LweI GCATC 2 cut(s) 456, 1216
MaeI CTAG 3 cut(s) 264, 681, 1263
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 3 cut(s) 636, 767, 942
MalI GATC 9 cut(s) 103, 462, 785, 832, 885, 1022, 1218, 1353, 1466
MboI GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
MfeI CAATTG 1 cut(s) 11
MflI RGATCY 4 cut(s) 101, 460, 783, 883
MlyI GAGTC 3 cut(s) 296, 299, 1391
MmeI TCCRAC 1 cut(s) 911
MroXI GAANNNNTTC 4 cut(s) 900, 985, 1239, 1551
MseI TTAA 5 cut(s) 366, 570, 723, 837, 906
MslI CAYNNNNRTG 1 cut(s) 375
MspA1I CMGCKG 2 cut(s) 992, 1391
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 1 cut(s) 891
MunI CAATTG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 871
MvnI CGCG 1 cut(s) 1391
MwoI GCNNNNNNNGC 3 cut(s) 998, 1007, 1043
NdeI CATATG 2 cut(s) 372, 1270
NdeII GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
NlaIII CATG 3 cut(s) 344, 1585, 1621
NlaIV GGNNCC 2 cut(s) 143, 785
NmeAIII GCCGAG 1 cut(s) 1063
NmuCI GTSAC 1 cut(s) 942
NspI RCATGY 1 cut(s) 1585
PaeR7I CTCGAG 1 cut(s) 464
PctI GAATGC 1 cut(s) 871
PdmI GAANNNNTTC 4 cut(s) 900, 985, 1239, 1551
PfeI GAWTC 6 cut(s) 243, 413, 713, 797, 896, 1613
PinAI ACCGGT 1 cut(s) 890
PkrI GCNGC 2 cut(s) 1000, 1048
PleI GAGTC 3 cut(s) 296, 298, 1390
PpsI GAGTC 3 cut(s) 296, 298, 1390
PsiI TTATAA 1 cut(s) 432
Psp1406I AACGTT 1 cut(s) 67
PspN4I GGNNCC 2 cut(s) 143, 785
PspPI GGNCC 2 cut(s) 739, 822
PstI CTGCAG 1 cut(s) 1411
PsuI RGATCY 4 cut(s) 101, 460, 783, 883
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 2 cut(s) 1073, 1483
RsaNI GTAC 2 cut(s) 1072, 1482
RseI CAYNNNNRTG 1 cut(s) 375
SacII CCGCGG 1 cut(s) 1392
SaqAI TTAA 5 cut(s) 366, 570, 723, 837, 906
SatI GCNGC 2 cut(s) 999, 1047
Sau3AI GATC 9 cut(s) 101, 460, 783, 830, 883, 1020, 1216, 1351, 1464
Sau96I GGNCC 2 cut(s) 739, 822
ScaI AGTACT 1 cut(s) 1483
SchI GAGTC 3 cut(s) 296, 299, 1391
SfaNI GCATC 2 cut(s) 456, 1216
SfcI CTRYAG 1 cut(s) 1407
Sfr274I CTCGAG 1 cut(s) 464
Sfr303I CCGCGG 1 cut(s) 1392
SgrBI CCGCGG 1 cut(s) 1392
SinI GGWCC 2 cut(s) 739, 822
SlaI CTCGAG 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 3 cut(s) 464, 836, 914
SmoI CTYRAG 3 cut(s) 464, 836, 914
SsiI CCGC 2 cut(s) 1389, 1391
SspMI CTAG 3 cut(s) 264, 681, 1263
StyI CCWWGG 2 cut(s) 498, 680
TaaI ACNGT 4 cut(s) 130, 422, 702, 812
TaiI ACGT 1 cut(s) 70
TaqI TCGA 7 cut(s) 165, 210, 246, 465, 829, 1350, 1367
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 1 cut(s) 1481
TfiI GAWTC 6 cut(s) 243, 413, 713, 797, 896, 1613
Tru1I TTAA 5 cut(s) 366, 570, 723, 837, 906
Tru9I TTAA 5 cut(s) 366, 570, 723, 837, 906
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 942
TseI GCWGC 2 cut(s) 998, 1046
Tsp45I GTSAC 1 cut(s) 942
TspDTI ATGAA 7 cut(s) 151, 498, 987, 1104, 1175, 1232, 1428
TspGWI ACGGA 2 cut(s) 814, 835
TspRI CASTG 1 cut(s) 133
Vha464I CTTAAG 1 cut(s) 836
VpaK11BI GGWCC 2 cut(s) 739, 822
XapI RAATTY 4 cut(s) 80, 203, 1178, 1573
XceI RCATGY 1 cut(s) 1585
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XhoI CTCGAG 1 cut(s) 464
XmaJI CCTAGG 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 1385
XmnI GAANNNNTTC 4 cut(s) 900, 985, 1239, 1551
XspI CTAG 3 cut(s) 264, 681, 1263
ZrmI AGTACT 1 cut(s) 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.