Rw7G002540

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011994
Physical Location & Seq
Forward (+)
2110549 .. 2113901
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002540.1

Sequence Viewer

Length: 2433 bp
ATGAGTTCAAACAAGAAAACAATAACAAAGGGACGCTTCACCCTTTATGTGGAGCGAAACATGGGAGAGCTCTCACGCTTCATGGTCTATGCTGTGCTTGAATGGGCCTTGATCATTCTGCTCTTCATTGATGGCCTTTTTGCTTTTGTTGCCAATGAATTCGCCAAGCTATTTGAGCTCAGATACCCCTGCTGGCTTTGCTCCAGGATCGATCACATTCTCGTTAACAGAGGTCGTGATTTCTATTATAATGATTCCATATGTGAATCACACAAGAAAGATGTCTCATACCTTGCTTACTGCCACAACCACAAGAGGCTATCCGACATAAGAAAGATGTGTGAGGCATGTCTTCTTTCATTTGCAACCGAAAAAGAGTCTGATTTCGACACATACAAGTCCCTAGTTGGGATCTTGCACAAGGATCTCGAGTGCTTTGTGGAGGATGATGAACATCAAATTCAGTTGAGTGTGCCTGCAGCAAGGACATGGGAGGATGCAGGGTCGATTGAGAAGAGTAGGGACAGCATGACCATAGGGTGTTCATGTTGCGGGGCGCCATTGAAGTTGAGTTCAGCTTCCTCCTACTCAAAAGCAAAGAGTGTTAGCGGGCTAGCGCAAGCTCCTACGCCTTCTCCACGAGCACCCGCAGGGAGAAATGATGATAATCCTGGTTTGGACTTGCCCCCTATTCGATATCCAGAACTCAAGTTTATGTCTGATAATGAGTCTGAGCTTCCAGAGGATGTGTATGGAACACACATGAAAAATCAAAAGGAAGATCCGAAAGCTGCTACATTGCCATTATTGACAGAAGGTGATGAGTTAGCTGATGATGCTAACAGGACTCCTATATATGGCAGAGGAAACAGGTTCTTTGGAATCCCACTTTCAGATTCAGCTCAAAATAGTCCTAGGGTGGCTATGAGGATTTCAAAGAAATCACCACTCGAAAAGAGCGAGTTTGCTTTGGAATCGGTTGATGGAAATCTTCCAAATGATGCAGATTGTGATTCAATTTTGCACCGTCTGAAGAGACAGGTCCGCTTGGACAGGAAGTCACTGATGGCTTTGTACATGGAACTCGATGAAGAAAGAAGTGCTTCAGCTGTAGCAGCCAACAATGCAATGGCTATGATCACAAGGCTACAAGCAGAGAAGGCTGCTGTTCAGATGGAGGCATTGCAATATCAGAGAATGATGGAGGAGCAGGCGGAGTATGACCAAGAAGCCCTGGAAGCAACATATGATTTGCTTGCCAAGAGAGAAGATGATCTCAGGGTTTTAGAGGCTGAAATAGAGGCTTATAGGGAGAAATATGGATTGTTGAAGGAATTTGTCTACAAGGACCAAGATGAGGCTGGTGAATGTGGTACTCCTCTGAGCGTAAAGTATGGCGCAAACAACATGCAAAATGATCAAGCCGGGTCAGCAAAGGAAGAGAATGCAGAGGCTGCCCACGGCGAAAGTTTGAAACCACTGAAAGGGGAGAAGACTTACCTACTTGGGCGGTTCAAGAAACTAGACAAGATGAGAAACTCATCATCTAATGGGTTCTTGCCAGATGGTCTGAATGAGGATGAGATAGGAAATGGAAACAAAGCTGCCCTAACAAGGCAATTGTCGCTGTTGTCTCATCTTAGTGATAGAGTGAAGGCTCTTGAATCTGACACCGGATTCCTAGAGTATGCTGCTAAGACACTTGAGAAACATAGTGAAGGAACAAAACTTTTGACAGAGATAACTCAAAATTTACAGAAGCTCCGGCACCTAGTGAATCTGCCTACTGAGGAACAAAACAAGACGCCTCCGGTGGAACAAAAGAAGGAGCAAAACAAGACGGATTCGGAGGAACAAAAGGAGCAAAACAAGACGCTTCCGGAGGAACAAAATAAGACGTCTCCGGAGGAACAAAAGGAGCAAAGTAAGACGCCTTCGGAGGAACAAAAGGAGCAAAACAAGACGCCTCCAGGGGAACAAAAGAAGGAGCAAAACAAGACGCCTCCGGAGGAACAAAATAAGGCGCATCCGGAGGAACAAAAGGAGCAAAGTAAGATGCCTTCGGAGGAACAAAAGGAGCAAAACAAGAAGCTTCCGGAGGATCAAAAGGAGCAAAACAAGACACTTCCGGAGGAACAAAAGAAGGAGCAAAGTAAGACGCCTCCAGAGGAACAAAAGGAGCGAAGTAAGACGCCTCCACAAGAACAAAAGAATGAGCAAAGTAAGACGCCATCCGAAGAACAAAATAAGGAGCAAAACAATATGCCCCCGGAGGAACAAAGTAAGACGCCTCCAGAGGAACAAAATGAGCAAAAGAAGACGCTTTCAGATGAACAAAATAAGATTCCTCCGGAAGAACAAAACAAAATGTCTCCAGAAGAACAGAAAAAGGAACAAAAAGACCTTACTTCTATAGTAAAAAGTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

810

Amino Acids

92.25

Weight (kDa)

5.17

Isoelectric Point (pI)

65.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 343 - 433 1.7e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AatII GACGTC 1 cut(s) 1901
AccB1I GGYRCC 2 cut(s) 556, 1767
AccI GTMKAC 1 cut(s) 1341
AccIII TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
AciI CCGC 6 cut(s) 552, 609, 648, 1045, 1214, 1510
AclWI GGATC 5 cut(s) 215, 419, 432, 776, 2109
AcsI RAATTY 4 cut(s) 158, 459, 1334, 1750
AcuI CTGAAG 2 cut(s) 1052, 1089
AdeI CACNNNGTG 1 cut(s) 1774
AfaI GTAC 2 cut(s) 1076, 1375
AfiI CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
AgsI TTSAA 9 cut(s) 9, 101, 565, 936, 1017, 1330, 1474, 1516, 1664
AhdI GACNNNNNGTC 1 cut(s) 1057
AjnI CCWGG 4 cut(s) 203, 670, 1233, 1969
Alw21I GWGCWC 3 cut(s) 72, 180, 646
Alw26I GTCTC 5 cut(s) 289, 1030, 1638, 1905, 2376
AlwI GGATC 5 cut(s) 215, 419, 432, 776, 2109
AlwNI CAGNNNCTG 1 cut(s) 1454
Ama87I CYCGRG 1 cut(s) 428
Aor13HI TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
AoxI GGCC 2 cut(s) 105, 133
ApeKI GCWGC 7 cut(s) 479, 791, 1115, 1163, 1454, 1604, 1691
ApoI RAATTY 4 cut(s) 158, 459, 1334, 1750
Asp700I GAANNNNTTC 1 cut(s) 1102
AspA2I CCTAGG 1 cut(s) 914
AspLEI GCGC 4 cut(s) 559, 619, 1400, 2026
AspS9I GGNCC 3 cut(s) 105, 1042, 1348
AsuC2I CCSGG 2 cut(s) 1426, 2270
AsuHPI GGTGA 4 cut(s) 31, 830, 936, 1376
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 1042, 1348
AvrII CCTAGG 1 cut(s) 914
BanI GGYRCC 2 cut(s) 556, 1767
BanII GRGCYC 2 cut(s) 72, 180
BarI GAAGNNNNNNTAC 4 cut(s) 1918, 1950, 2044, 2076
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 3 cut(s) 344, 1499, 2324
Bbv12I GWGCWC 3 cut(s) 72, 180, 646
BbvI GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
BccI CCATC 7 cut(s) 125, 977, 1060, 1168, 1195, 1559, 2239
BceAI ACGGC 1 cut(s) 1477
BciT130I CCWGG 4 cut(s) 205, 672, 1235, 1971
BclI TGATCA 3 cut(s) 111, 1137, 1417
BcnI CCSGG 2 cut(s) 1426, 2270
BcoDI GTCTC 5 cut(s) 289, 1030, 1638, 1905, 2376
BfaI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
BfmI CTRYAG 3 cut(s) 477, 1110, 2412
BfoI RGCGCY 1 cut(s) 560
BisI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
BlnI CCTAGG 1 cut(s) 914
BlsI GCNGC 7 cut(s) 481, 793, 1117, 1165, 1456, 1606, 1693
Bme1390I CCNGG 6 cut(s) 205, 672, 1235, 1426, 1971, 2270
Bme18I GGWCC 2 cut(s) 1042, 1348
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 105, 1042, 1348
BmiI GGNNCC 2 cut(s) 558, 1769
BmrFI CCNGG 6 cut(s) 205, 672, 1235, 1426, 1971, 2270
BmsI GCATC 5 cut(s) 487, 826, 991, 2035, 2046
BmtI GCTAGC 1 cut(s) 617
BpiI GAAGAC 3 cut(s) 344, 1499, 2324
BpmI CTGGAG 5 cut(s) 187, 1953, 2148, 2277, 2358
BpuEI CTTGAG 2 cut(s) 692, 1724
BpuMI CCSGG 2 cut(s) 1426, 2270
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 3 cut(s) 453, 666, 987
BsaJI CCNNGG 5 cut(s) 914, 1233, 1459, 1970, 2268
BsaWI WCCGGW 9 cut(s) 1673, 1810, 1879, 1903, 2005, 2029, 2095, 2128, 2350
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 1746, 1776
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
Bse3DI GCAATG 3 cut(s) 797, 1134, 1181
Bse8I GATNNNNATC 3 cut(s) 453, 666, 987
BseAI TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
BseBI CCWGG 4 cut(s) 205, 672, 1235, 1971
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 5 cut(s) 914, 1233, 1459, 1970, 2268
BseGI GGATG 6 cut(s) 451, 502, 751, 1585, 2026, 2231
BseJI GATNNNNATC 3 cut(s) 453, 666, 987
BseLI CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
BseMI GCAATG 3 cut(s) 797, 1134, 1181
BseMII CTCAG 5 cut(s) 193, 723, 1291, 1373, 1779
BseRI GAGGAG 2 cut(s) 1220, 1368
BseXI GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
BshFI GGCC 2 cut(s) 107, 135
BshNI GGYRCC 2 cut(s) 556, 1767
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 3 cut(s) 72, 180, 646
BsiHKCI CYCGRG 1 cut(s) 428
BslFI GGGAC 3 cut(s) 45, 385, 536
BslI CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
BsmAI GTCTC 5 cut(s) 289, 1030, 1638, 1905, 2376
BsmBI CGTCTC 1 cut(s) 1905
BsmFI GGGAC 3 cut(s) 45, 385, 536
BsmI GAATGC 1 cut(s) 1450
BsnI GGCC 2 cut(s) 107, 135
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 72, 180, 646
Bsp13I TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
Bsp1407I TGTACA 1 cut(s) 1074
BspACI CCGC 6 cut(s) 552, 609, 648, 1045, 1214, 1510
BspANI GGCC 2 cut(s) 107, 135
BspCNI CTCAG 5 cut(s) 192, 724, 1290, 1374, 1780
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
BspLI GGNNCC 2 cut(s) 558, 1769
BspMAI CTGCAG 1 cut(s) 481
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 5 cut(s) 215, 419, 432, 776, 2109
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 2 cut(s) 556, 1767
BsrDI GCAATG 3 cut(s) 797, 1134, 1181
BsrGI TGTACA 1 cut(s) 1074
BssECI CCNNGG 5 cut(s) 914, 1233, 1459, 1970, 2268
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 914
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 4 cut(s) 205, 672, 1235, 1971
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 4 cut(s) 128, 509, 1028, 1434
BstAPI GCANNNNNTGC 1 cut(s) 1454
BstAUI TGTACA 1 cut(s) 1074
BstC8I GCNNGC 7 cut(s) 194, 477, 611, 615, 621, 1212, 1257
BstDEI CTNAG 7 cut(s) 179, 732, 1277, 1382, 1640, 1695, 1788
BstDSI CCRYGG 1 cut(s) 1459
BstF5I GGATG 6 cut(s) 451, 502, 751, 1585, 2026, 2231
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 4 cut(s) 559, 619, 1400, 2026
BstMAI GTCTC 5 cut(s) 289, 1030, 1638, 1905, 2376
BstNI CCWGG 4 cut(s) 205, 672, 1235, 1971
BstNSI RCATGY 2 cut(s) 351, 1411
BstSCI CCNGG 6 cut(s) 203, 670, 1233, 1424, 1969, 2268
BstSFI CTRYAG 3 cut(s) 477, 1110, 2412
BstV1I GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
BstV2I GAAGAC 3 cut(s) 344, 1499, 2324
BstX2I RGATCY 3 cut(s) 411, 424, 781
BstYI RGATCY 3 cut(s) 411, 424, 781
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 2 cut(s) 107, 135
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 1459
BtsCI GGATG 6 cut(s) 451, 502, 751, 1585, 2026, 2231
BtsIMutI CAGTG 2 cut(s) 1061, 1478
Cac8I GCNNGC 7 cut(s) 194, 477, 611, 615, 621, 1212, 1257
CaiI CAGNNNCTG 1 cut(s) 1454
CfoI GCGC 4 cut(s) 559, 619, 1400, 2026
Cfr13I GGNCC 3 cut(s) 105, 1042, 1348
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 2 cut(s) 1075, 1374
CviAII CATG 9 cut(s) 61, 82, 348, 489, 529, 546, 763, 1078, 1408
CviQI GTAC 2 cut(s) 1075, 1374
DdeI CTNAG 7 cut(s) 179, 732, 1277, 1382, 1640, 1695, 1788
DinI GGCGCC 1 cut(s) 558
DraIII CACNNNGTG 1 cut(s) 1774
DriI GACNNNNNGTC 1 cut(s) 1057
Eam1104I CTCTTC 4 cut(s) 128, 509, 1028, 1434
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 4 cut(s) 128, 509, 1028, 1434
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 2 cut(s) 70, 178
Eco130I CCWWGG 1 cut(s) 914
Eco24I GRGCYC 2 cut(s) 72, 180
Eco32I GATATC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 1042, 1348
Eco53kI GAGCTC 2 cut(s) 70, 178
Eco57I CTGAAG 2 cut(s) 1052, 1089
Eco88I CYCGRG 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 70, 178
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 4 cut(s) 203, 670, 1233, 1969
EcoRV GATATC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 914
EcoT38I GRGCYC 2 cut(s) 72, 180
EgeI GGCGCC 1 cut(s) 558
EheI GGCGCC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 914
Esp3I CGTCTC 1 cut(s) 1905
FaeI CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 1087, 1119
FaqI GGGAC 3 cut(s) 45, 385, 536
FatI CATG 9 cut(s) 60, 81, 347, 488, 528, 545, 762, 1077, 1407
FauI CCCGC 3 cut(s) 545, 602, 655
FauNDI CATATG 2 cut(s) 260, 1246
FbaI TGATCA 3 cut(s) 111, 1137, 1417
FblI GTMKAC 1 cut(s) 1341
Fnu4HI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
FokI GGATG 6 cut(s) 458, 509, 758, 1592, 2013, 2218
FriOI GRGCYC 2 cut(s) 72, 180
Fsp4HI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
FspBI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
GlaI GCGC 4 cut(s) 558, 618, 1399, 2025
GluI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
GsuI CTGGAG 5 cut(s) 187, 1953, 2148, 2277, 2358
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 2 cut(s) 107, 135
HhaI GCGC 4 cut(s) 559, 619, 1400, 2026
Hin1II CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
Hin6I GCGC 4 cut(s) 557, 617, 1398, 2024
HinP1I GCGC 4 cut(s) 557, 617, 1398, 2024
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 2090
HpaI GTTAAC 1 cut(s) 226
HphI GGTGA 4 cut(s) 31, 830, 936, 1376
Hpy166II GTNNAC 2 cut(s) 226, 1342
Hpy8I GTNNAC 2 cut(s) 226, 1342
HpyCH4III ACNGT 1 cut(s) 1028
HpyCH4IV ACGT 1 cut(s) 1898
HpyF3I CTNAG 7 cut(s) 179, 732, 1277, 1382, 1640, 1695, 1788
HpySE526I ACGT 1 cut(s) 1898
Hsp92II CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
HspAI GCGC 4 cut(s) 557, 617, 1398, 2024
KasI GGCGCC 1 cut(s) 556
Kpn2I TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
Ksp22I TGATCA 3 cut(s) 111, 1137, 1417
KspAI GTTAAC 1 cut(s) 226
LguI GCTCTTC 1 cut(s) 128
Lsp1109I GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
LweI GCATC 5 cut(s) 487, 826, 991, 2035, 2046
MaeI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
MaeII ACGT 1 cut(s) 1898
MaeIII GTNAC 1 cut(s) 1059
MfeI CAATTG 1 cut(s) 1619
MflI RGATCY 3 cut(s) 411, 424, 781
MhlI GDGCHC 3 cut(s) 72, 180, 646
MluCI AATT 6 cut(s) 158, 459, 1017, 1334, 1619, 1750
Mly113I GGCGCC 1 cut(s) 557
MlyI GAGTC 3 cut(s) 386, 737, 841
MmeI TCCRAC 1 cut(s) 348
MroI TCCGGA 7 cut(s) 1879, 1903, 2005, 2029, 2095, 2128, 2350
MroXI GAANNNNTTC 1 cut(s) 1102
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 1 cut(s) 1641
MspA1I CMGCKG 1 cut(s) 1109
MspR9I CCNGG 6 cut(s) 205, 672, 1235, 1426, 1971, 2270
MunI CAATTG 1 cut(s) 1619
Mva1269I GAATGC 1 cut(s) 1450
MvaI CCWGG 4 cut(s) 205, 672, 1235, 1971
NarI GGCGCC 1 cut(s) 557
NciI CCSGG 2 cut(s) 1426, 2270
NdeI CATATG 2 cut(s) 260, 1246
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
NlaIV GGNNCC 2 cut(s) 558, 1769
NmuCI GTSAC 1 cut(s) 1059
NspI RCATGY 2 cut(s) 351, 1411
PaeR7I CTCGAG 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PctI GAATGC 1 cut(s) 1450
PdmI GAANNNNTTC 1 cut(s) 1102
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 7 cut(s) 481, 793, 1117, 1165, 1456, 1606, 1693
PleI GAGTC 3 cut(s) 385, 736, 841
PluTI GGCGCC 1 cut(s) 560
PpsI GAGTC 3 cut(s) 385, 736, 841
PsiI TTATAA 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 72, 180
Psp6I CCWGG 4 cut(s) 203, 670, 1233, 1969
PspGI CCWGG 4 cut(s) 203, 670, 1233, 1969
PspN4I GGNNCC 2 cut(s) 558, 1769
PspPI GGNCC 3 cut(s) 105, 1042, 1348
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 1454
PsuI RGATCY 3 cut(s) 411, 424, 781
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 2 cut(s) 1076, 1375
RsaNI GTAC 2 cut(s) 1075, 1374
RseI CAYNNNNRTG 1 cut(s) 1641
SacI GAGCTC 2 cut(s) 72, 180
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
Sau96I GGNCC 3 cut(s) 105, 1042, 1348
SchI GAGTC 3 cut(s) 386, 737, 841
ScrFI CCNGG 6 cut(s) 205, 672, 1235, 1426, 1971, 2270
SduI GDGCHC 3 cut(s) 72, 180, 646
SfaNI GCATC 5 cut(s) 487, 826, 991, 2035, 2046
SfcI CTRYAG 3 cut(s) 477, 1110, 2412
SfoI GGCGCC 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 2 cut(s) 1042, 1348
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 1641
SmlI CTYRAG 3 cut(s) 428, 707, 1703
SmoI CTYRAG 3 cut(s) 428, 707, 1703
Sse9I AATT 6 cut(s) 158, 459, 1017, 1334, 1619, 1750
SsiI CCGC 6 cut(s) 552, 609, 648, 1045, 1214, 1510
SspDI GGCGCC 1 cut(s) 556
SspMI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
SstI GAGCTC 2 cut(s) 72, 180
StyD4I CCNGG 6 cut(s) 203, 670, 1233, 1424, 1969, 2268
StyI CCWWGG 1 cut(s) 914
TaaI ACNGT 1 cut(s) 1028
TaiI ACGT 1 cut(s) 1901
TaqI TCGA 7 cut(s) 210, 387, 429, 506, 694, 951, 1086
TasI AATT 6 cut(s) 158, 459, 1017, 1334, 1619, 1750
TatI WGTACW 1 cut(s) 1074
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 2 cut(s) 1068, 1485
TseFI GTSAC 1 cut(s) 1059
TseI GCWGC 7 cut(s) 479, 791, 1115, 1163, 1454, 1604, 1691
Tsp45I GTSAC 1 cut(s) 1059
TspDTI ATGAA 9 cut(s) 70, 115, 171, 348, 465, 534, 779, 1104, 2346
TspGWI ACGGA 1 cut(s) 1856
TspRI CASTG 2 cut(s) 1068, 1485
VpaK11BI GGWCC 2 cut(s) 1042, 1348
XapI RAATTY 4 cut(s) 158, 459, 1334, 1750
XceI RCATGY 2 cut(s) 351, 1411
XcmI CCANNNNNNNNNTGG 2 cut(s) 1126, 1358
XhoI CTCGAG 1 cut(s) 428
XmaJI CCTAGG 1 cut(s) 914
XmiI GTMKAC 1 cut(s) 1341
XmnI GAANNNNTTC 1 cut(s) 1102
XspI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
ZraI GACGTC 1 cut(s) 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.