Rroxscaffold_3G00273160

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011994
Physical Location & Seq
Reverse (-)
65028170 .. 65030996
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273160.1

Sequence Viewer

Length: 2298 bp
ATGCCAAAGGGACGCTTCACCCTTTATGTGGAGCGAAACATGGGAGAGCTCTCACGCTTCATGGTCTATGCTGTTCTTGAATGGGTCTTGATCATTCTGCTCTTCATTGATGGCTTTTTTGCTTTTGTTGCCAATGAATTCGCCAAGCTATTTGAGCTCAGATACCCCTGCTGGCTTTGCACCAGGATCGATCACATTCTCGTTAACAGAGGTCGTGATTTCTATTATAATGATTCCATATGTGAATCACACAAGAAAGATGTCTCATACCTTGCTTACTGCCACAACCACAAGAGGCTATCCGACATAAGAAAGATGTGTGAGGCATGTCTTCTTTCATTTGCAACAGAAAAAGAGTCTGATTTCGACACATACAAGTCCCTAGTTGGGATCTTACACAAGGATCTCGAGTGCTTGGTGGAGGATGATGAACATCAAATTCAGCTGAGTGTGCCTGCAGCAAGGACATGGGAGGATGCAGGGTCGATTGAGAAGAGTAGGGACAGCATGACCATAGGGTGTTCTTGTTGCGGTGCGCCATTGAAGTTGAGTTCAGCTTCCTCCTACTCAAAAGCAAAGAGTGTTAGCGGGCTAGCGCAAGCTCCTACGCCTTCTCCACGAGCACCCCCAGGGAGAAATGATGATAATCCTGGTTTGGACTTGCCCCCTATTCGATACCCAGAACTCAAGTTTATGTCTGATAATGAGTCTGAGCTTCCAGAGGATGAGTATGGAACACATACGAAAAATCAAAAGGAAGATCCGAAAGCTGCTACATTGCCATTATTGTCAGAAGGTGATGAGTTAACTGATGATGCTAATAGGACTCCTATATATGGCAGAGGAAACAGGTTCTTTGGAATCCCACTTTCAGATTCAGCTCAAAATAGTCCTAGGGTGGCTATGAGGATTTCAAAGAAATCACCACTCGAAAAGAGTGAGTTTGCTTTGGAATCGGTTGATGGAAATCTTCCAAATGATGCGGATTGTGATTCAATTTTGCACCGTCTGAAGAGACAGGTCCGCGTGGACAGGAAGTCACTGATGGCTTTGTACATGGAACTCGATGAAGAAAGAAGTGCTTCAGCTGTAGCAGCCAACAATGCAATGGCTATGATCACAAGGCTACAAGCAGAGAAGGCTGCTGTTCAGATGGAGGCATTGCAATATCAGAGAATGATGGAGGAGCAGGCGGAGTATGACCAAGAGGCCCTGGAAGCAACGTATGATTTGCTTGCCAAGAGAGAAGATGATCTCAGGGTTTTAGAGGCTGAAATAGAGGCTTATAGGGAGAAATATGGATTGTTGAAGGAATTTGTCTACAAGGACCAAGATGAGGCTGGTGAATGTGGTACTCCTCTAAGCGTAAAGTATGGCGCAAACAACATGCAAAATGATCAAGCCGGGTCAGCAAAGGAGGAAAATGCAGACGCTGCCCTCGGTGAAAGTTTGAAACCACTAAAAGGGGAGAAGACTTACCTACTTGGGCGGTTCAAGAAACTAGACAAGATGAGAAACTCATCATCTAATGGGTTCTTGCCAGATGGTCTGAATGAGGATGAGATCGGAAATGGAAACAAAGCTGCCCTAACAAGGCAATTGTCGCTGTTGTCTCATCTTAGTGATAGAGTGAAGGCTCTTGAATCTGATACTGGATTCTTAGAGTATGCTGCTAAGACACTTGAGAAACATAGTGAAGGAACAAAACTTTTGACAGAGATAACTCAAAATTTACAGAAGCTCCGGCACCTTGTGAATCTTCCTACTGAGGAACAAAACAAGACGCCTCCGGCGGAACAAAAAAAGGAGCAAAACAAGACGCCTTCGGTGGAAAAAAAGGAGCAAAACAAGACGCATCCGGAGGAACAAAAGAAGGAGCAATGTAAGACGCTTTCAGAGGAACAAAAGGAGCAAAACAAGACGCCTCTGGAGGAACAAAATAAGACATCTCCGGAGGAACAAAAGAAGGAGCAAAGTAAGACGCCTGTAGAGGAACAAAAGAATGAGCAAAGTAAGACGCCTCCAAAGGAACAAAAGGAGCAAAACAATACGCCTCTGGAGGAACAAAAGAAGGAGCAAAACAATACTCTGGAGGAACAAAAGAATGAGCAAAGTAAGACGCCTTCGGAGGAACAAAAGGAGCAAAACAAGACACCTTCGGAGGAACAAAATGAGCAAAAGAAAATGCTTTCGGATGAACAAAATAAGATTCCTCCGGAAGAACAAAACAAGACGTCTCCAGAAGAACAGAAAAAGGAACAAAAAGACCTTACTTCCATAGTAAAAAGTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

765

Amino Acids

87.09

Weight (kDa)

5.16

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 336 - 426 1.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AatII GACGTC 1 cut(s) 2237
AccB1I GGYRCC 1 cut(s) 1746
AccI GTMKAC 1 cut(s) 1320
AccII CGCG 1 cut(s) 1026
AccIII TCCGGA 3 cut(s) 1858, 1951, 2215
AciI CCGC 7 cut(s) 531, 588, 983, 1024, 1193, 1489, 1793
AclWI GGATC 4 cut(s) 194, 398, 411, 755
AcsI RAATTY 4 cut(s) 137, 438, 1313, 1729
AcuI CTGAAG 2 cut(s) 1031, 1068
AcyI GRCGYC 7 cut(s) 1784, 1820, 1922, 1982, 2018, 2120, 2234
AdeI CACNNNGTG 1 cut(s) 1753
AfaI GTAC 2 cut(s) 1055, 1354
AfiI CCNNNNNNNGG 6 cut(s) 28, 387, 836, 1336, 1463, 1593
AgsI TTSAA 8 cut(s) 80, 544, 915, 996, 1309, 1453, 1495, 1643
AhdI GACNNNNNGTC 1 cut(s) 1036
AjnI CCWGG 4 cut(s) 182, 628, 649, 1212
Alw21I GWGCWC 3 cut(s) 51, 159, 625
Alw26I GTCTC 4 cut(s) 268, 1009, 1617, 2241
AlwI GGATC 4 cut(s) 194, 398, 411, 755
AlwNI CAGNNNCTG 1 cut(s) 1433
Ama87I CYCGRG 1 cut(s) 407
Aor13HI TCCGGA 3 cut(s) 1858, 1951, 2215
AoxI GGCC 1 cut(s) 1209
ApeKI GCWGC 7 cut(s) 458, 770, 1094, 1142, 1433, 1583, 1670
ApoI RAATTY 4 cut(s) 137, 438, 1313, 1729
Asp700I GAANNNNTTC 1 cut(s) 1081
AspA2I CCTAGG 1 cut(s) 893
AspLEI GCGC 3 cut(s) 538, 598, 1379
AspS9I GGNCC 3 cut(s) 1021, 1210, 1327
AsuC2I CCSGG 1 cut(s) 1405
AsuHPI GGTGA 5 cut(s) 10, 809, 915, 1355, 1454
AsuNHI GCTAGC 1 cut(s) 592
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 1021, 1327
AvrII CCTAGG 1 cut(s) 893
BanI GGYRCC 1 cut(s) 1746
BanII GRGCYC 2 cut(s) 51, 159
BarI GAAGNNNNNNTAC 2 cut(s) 2107, 2139
BauI CACGAG 1 cut(s) 618
BbsI GAAGAC 2 cut(s) 323, 1478
Bbv12I GWGCWC 3 cut(s) 51, 159, 625
BbvI GCAGC 7 cut(s) 470, 757, 1106, 1129, 1420, 1570, 1657
BccI CCATC 6 cut(s) 104, 956, 1039, 1147, 1174, 1538
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciT130I CCWGG 4 cut(s) 184, 630, 651, 1214
BclI TGATCA 3 cut(s) 90, 1116, 1396
BcnI CCSGG 1 cut(s) 1405
BcoDI GTCTC 4 cut(s) 268, 1009, 1617, 2241
BfaI CTAG 4 cut(s) 383, 593, 894, 1502
BfmI CTRYAG 3 cut(s) 456, 1089, 1986
BisI GCNGC 7 cut(s) 459, 771, 1095, 1143, 1434, 1584, 1671
BlnI CCTAGG 1 cut(s) 893
BlsI GCNGC 7 cut(s) 460, 772, 1096, 1144, 1435, 1585, 1672
Bme1390I CCNGG 5 cut(s) 184, 630, 651, 1214, 1405
Bme18I GGWCC 2 cut(s) 1021, 1327
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 1021, 1210, 1327
BmiI GGNNCC 1 cut(s) 1748
BmrFI CCNGG 5 cut(s) 184, 630, 651, 1214, 1405
BmsI GCATC 4 cut(s) 466, 805, 970, 1864
BmtI GCTAGC 1 cut(s) 596
BpiI GAAGAC 2 cut(s) 323, 1478
BpmI CTGGAG 4 cut(s) 1949, 2078, 2111, 2223
BpuEI CTTGAG 2 cut(s) 671, 1703
BpuMI CCSGG 1 cut(s) 1405
Bsa29I ATCGAT 1 cut(s) 189
BsaBI GATNNNNATC 3 cut(s) 432, 645, 966
BsaHI GRCGYC 7 cut(s) 1784, 1820, 1922, 1982, 2018, 2120, 2234
BsaJI CCNNGG 5 cut(s) 628, 629, 893, 1212, 1438
BsaWI WCCGGW 3 cut(s) 1858, 1951, 2215
BsaXI ACNNNNNCTCC 4 cut(s) 598, 628, 1725, 1755
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 387, 836, 1336, 1463, 1593
Bse1I ACTGG 1 cut(s) 1657
Bse3DI GCAATG 4 cut(s) 776, 1113, 1160, 1886
Bse8I GATNNNNATC 3 cut(s) 432, 645, 966
BseAI TCCGGA 3 cut(s) 1858, 1951, 2215
BseBI CCWGG 4 cut(s) 184, 630, 651, 1214
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 5 cut(s) 628, 629, 893, 1212, 1438
BseGI GGATG 6 cut(s) 430, 481, 730, 1564, 1855, 2200
BseJI GATNNNNATC 3 cut(s) 432, 645, 966
BseLI CCNNNNNNNGG 6 cut(s) 28, 387, 836, 1336, 1463, 1593
BseMI GCAATG 4 cut(s) 776, 1113, 1160, 1886
BseMII CTCAG 5 cut(s) 172, 437, 702, 1270, 1758
BseNI ACTGG 1 cut(s) 1657
BseRI GAGGAG 2 cut(s) 1199, 1347
BseXI GCAGC 7 cut(s) 470, 757, 1106, 1129, 1420, 1570, 1657
Bsh1236I CGCG 1 cut(s) 1026
BshFI GGCC 1 cut(s) 1211
BshNI GGYRCC 1 cut(s) 1746
BshVI ATCGAT 1 cut(s) 189
BsiHKAI GWGCWC 3 cut(s) 51, 159, 625
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 6 cut(s) 1404, 1744, 1790, 1859, 1952, 2216
BslFI GGGAC 3 cut(s) 24, 364, 515
BslI CCNNNNNNNGG 6 cut(s) 28, 387, 836, 1336, 1463, 1593
BsmAI GTCTC 4 cut(s) 268, 1009, 1617, 2241
BsmBI CGTCTC 1 cut(s) 2241
BsmFI GGGAC 3 cut(s) 24, 364, 515
BsnI GGCC 1 cut(s) 1211
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 3 cut(s) 51, 159, 625
Bsp13I TCCGGA 3 cut(s) 1858, 1951, 2215
Bsp1407I TGTACA 1 cut(s) 1053
BspACI CCGC 7 cut(s) 531, 588, 983, 1024, 1193, 1489, 1793
BspANI GGCC 1 cut(s) 1211
BspCNI CTCAG 5 cut(s) 171, 438, 703, 1269, 1759
BspDI ATCGAT 1 cut(s) 189
BspEI TCCGGA 3 cut(s) 1858, 1951, 2215
BspFNI CGCG 1 cut(s) 1026
BspLI GGNNCC 1 cut(s) 1748
BspMAI CTGCAG 1 cut(s) 460
BspOI GCTAGC 1 cut(s) 596
BspPI GGATC 4 cut(s) 194, 398, 411, 755
BspQI GCTCTTC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 1746
BsrDI GCAATG 4 cut(s) 776, 1113, 1160, 1886
BsrGI TGTACA 1 cut(s) 1053
BsrI ACTGG 1 cut(s) 1657
BssECI CCNNGG 5 cut(s) 628, 629, 893, 1212, 1438
BssNI GRCGYC 7 cut(s) 1784, 1820, 1922, 1982, 2018, 2120, 2234
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 893
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 4 cut(s) 184, 630, 651, 1214
Bst4CI ACNGT 1 cut(s) 1007
Bst6I CTCTTC 3 cut(s) 107, 488, 1007
BstACI GRCGYC 7 cut(s) 1784, 1820, 1922, 1982, 2018, 2120, 2234
BstAPI GCANNNNNTGC 1 cut(s) 1433
BstAUI TGTACA 1 cut(s) 1053
BstC8I GCNNGC 7 cut(s) 173, 456, 590, 594, 600, 1191, 1236
BstDEI CTNAG 9 cut(s) 158, 446, 711, 1256, 1361, 1619, 1660, 1674, 1767
BstF5I GGATG 6 cut(s) 430, 481, 730, 1564, 1855, 2200
BstFNI CGCG 1 cut(s) 1026
BstHHI GCGC 3 cut(s) 538, 598, 1379
BstMAI GTCTC 4 cut(s) 268, 1009, 1617, 2241
BstNI CCWGG 4 cut(s) 184, 630, 651, 1214
BstNSI RCATGY 2 cut(s) 330, 1390
BstSCI CCNGG 5 cut(s) 182, 628, 649, 1212, 1403
BstSFI CTRYAG 3 cut(s) 456, 1089, 1986
BstUI CGCG 1 cut(s) 1026
BstV1I GCAGC 7 cut(s) 470, 757, 1106, 1129, 1420, 1570, 1657
BstV2I GAAGAC 2 cut(s) 323, 1478
BstX2I RGATCY 3 cut(s) 390, 403, 760
BstYI RGATCY 3 cut(s) 390, 403, 760
Bsu15I ATCGAT 1 cut(s) 189
BsuRI GGCC 1 cut(s) 1211
BsuTUI ATCGAT 1 cut(s) 189
BtsCI GGATG 6 cut(s) 430, 481, 730, 1564, 1855, 2200
BtsIMutI CAGTG 1 cut(s) 1040
Cac8I GCNNGC 7 cut(s) 173, 456, 590, 594, 600, 1191, 1236
CaiI CAGNNNCTG 1 cut(s) 1433
CfoI GCGC 3 cut(s) 538, 598, 1379
Cfr13I GGNCC 3 cut(s) 1021, 1210, 1327
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 2 cut(s) 1054, 1353
CviAII CATG 7 cut(s) 40, 61, 327, 468, 508, 1057, 1387
CviQI GTAC 2 cut(s) 1054, 1353
DdeI CTNAG 9 cut(s) 158, 446, 711, 1256, 1361, 1619, 1660, 1674, 1767
DraIII CACNNNGTG 1 cut(s) 1753
DriI GACNNNNNGTC 1 cut(s) 1036
Eam1104I CTCTTC 3 cut(s) 107, 488, 1007
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 3 cut(s) 107, 488, 1007
EciI GGCGGA 2 cut(s) 1208, 1808
Ecl136II GAGCTC 2 cut(s) 49, 157
Eco130I CCWWGG 1 cut(s) 893
Eco24I GRGCYC 2 cut(s) 51, 159
Eco47I GGWCC 2 cut(s) 1021, 1327
Eco53kI GAGCTC 2 cut(s) 49, 157
Eco57I CTGAAG 2 cut(s) 1031, 1068
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 2 cut(s) 49, 157
EcoO109I RGGNCCY 1 cut(s) 1210
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 4 cut(s) 182, 628, 649, 1212
EcoT14I CCWWGG 1 cut(s) 893
EcoT38I GRGCYC 2 cut(s) 51, 159
ErhI CCWWGG 1 cut(s) 893
Esp3I CGTCTC 1 cut(s) 2241
FaeI CATG 7 cut(s) 43, 64, 330, 471, 511, 1060, 1390
FalI AAGNNNNNCTT 3 cut(s) 31, 1066, 1098
FaqI GGGAC 3 cut(s) 24, 364, 515
FatI CATG 7 cut(s) 39, 60, 326, 467, 507, 1056, 1386
FauI CCCGC 1 cut(s) 581
FauNDI CATATG 1 cut(s) 239
FbaI TGATCA 3 cut(s) 90, 1116, 1396
FblI GTMKAC 1 cut(s) 1320
Fnu4HI GCNGC 7 cut(s) 459, 771, 1095, 1143, 1434, 1584, 1671
FokI GGATG 6 cut(s) 437, 488, 737, 1571, 1842, 2207
FriOI GRGCYC 2 cut(s) 51, 159
Fsp4HI GCNGC 7 cut(s) 459, 771, 1095, 1143, 1434, 1584, 1671
FspBI CTAG 4 cut(s) 383, 593, 894, 1502
GlaI GCGC 3 cut(s) 537, 597, 1378
GluI GCNGC 7 cut(s) 459, 771, 1095, 1143, 1434, 1584, 1671
GsuI CTGGAG 4 cut(s) 1949, 2078, 2111, 2223
HaeIII GGCC 1 cut(s) 1211
HapII CCGG 6 cut(s) 1404, 1744, 1790, 1859, 1952, 2216
HhaI GCGC 3 cut(s) 538, 598, 1379
Hin1I GRCGYC 7 cut(s) 1784, 1820, 1922, 1982, 2018, 2120, 2234
Hin1II CATG 7 cut(s) 43, 64, 330, 471, 511, 1060, 1390
Hin6I GCGC 3 cut(s) 536, 596, 1377
HinP1I GCGC 3 cut(s) 536, 596, 1377
HincII GTYRAC 2 cut(s) 205, 807
HindII GTYRAC 2 cut(s) 205, 807
HpaI GTTAAC 2 cut(s) 205, 807
HpaII CCGG 6 cut(s) 1404, 1744, 1790, 1859, 1952, 2216
HphI GGTGA 5 cut(s) 10, 809, 915, 1355, 1454
Hpy166II GTNNAC 4 cut(s) 205, 807, 1030, 1321
Hpy8I GTNNAC 4 cut(s) 205, 807, 1030, 1321
HpyCH4III ACNGT 1 cut(s) 1007
HpyCH4IV ACGT 2 cut(s) 1223, 2234
HpyCH4V TGCA 9 cut(s) 180, 344, 458, 479, 1003, 1106, 1165, 1390, 1427
HpyF3I CTNAG 9 cut(s) 158, 446, 711, 1256, 1361, 1619, 1660, 1674, 1767
HpySE526I ACGT 2 cut(s) 1223, 2234
Hsp92I GRCGYC 7 cut(s) 1784, 1820, 1922, 1982, 2018, 2120, 2234
Hsp92II CATG 7 cut(s) 43, 64, 330, 471, 511, 1060, 1390
HspAI GCGC 3 cut(s) 536, 596, 1377
Kpn2I TCCGGA 3 cut(s) 1858, 1951, 2215
Ksp22I TGATCA 3 cut(s) 90, 1116, 1396
KspAI GTTAAC 2 cut(s) 205, 807
LguI GCTCTTC 1 cut(s) 107
Lsp1109I GCAGC 7 cut(s) 470, 757, 1106, 1129, 1420, 1570, 1657
LweI GCATC 4 cut(s) 466, 805, 970, 1864
MaeI CTAG 4 cut(s) 383, 593, 894, 1502
MaeII ACGT 2 cut(s) 1223, 2234
MaeIII GTNAC 1 cut(s) 1038
MfeI CAATTG 1 cut(s) 1598
MflI RGATCY 3 cut(s) 390, 403, 760
MhlI GDGCHC 3 cut(s) 51, 159, 625
MluCI AATT 6 cut(s) 137, 438, 996, 1313, 1598, 1729
MlyI GAGTC 3 cut(s) 365, 716, 820
MmeI TCCRAC 1 cut(s) 327
MroI TCCGGA 3 cut(s) 1858, 1951, 2215
MroXI GAANNNNTTC 1 cut(s) 1081
MseI TTAA 2 cut(s) 204, 806
MslI CAYNNNNRTG 1 cut(s) 1620
MspA1I CMGCKG 2 cut(s) 445, 1088
MspI CCGG 6 cut(s) 1404, 1744, 1790, 1859, 1952, 2216
MspR9I CCNGG 5 cut(s) 184, 630, 651, 1214, 1405
MunI CAATTG 1 cut(s) 1598
MvaI CCWGG 4 cut(s) 184, 630, 651, 1214
MvnI CGCG 1 cut(s) 1026
NciI CCSGG 1 cut(s) 1405
NdeI CATATG 1 cut(s) 239
NheI GCTAGC 1 cut(s) 592
NlaIII CATG 7 cut(s) 43, 64, 330, 471, 511, 1060, 1390
NlaIV GGNNCC 1 cut(s) 1748
NmuCI GTSAC 1 cut(s) 1038
NspI RCATGY 2 cut(s) 330, 1390
PaeR7I CTCGAG 1 cut(s) 407
PasI CCCWGGG 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 1081
PkrI GCNGC 7 cut(s) 460, 772, 1096, 1144, 1435, 1585, 1672
PleI GAGTC 3 cut(s) 364, 715, 820
PpsI GAGTC 3 cut(s) 364, 715, 820
PsiI TTATAA 1 cut(s) 228
Psp124BI GAGCTC 2 cut(s) 51, 159
Psp6I CCWGG 4 cut(s) 182, 628, 649, 1212
PspGI CCWGG 4 cut(s) 182, 628, 649, 1212
PspN4I GGNNCC 1 cut(s) 1748
PspPI GGNCC 3 cut(s) 1021, 1210, 1327
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 1433
PsuI RGATCY 3 cut(s) 390, 403, 760
PvuII CAGCTG 2 cut(s) 445, 1088
RsaI GTAC 2 cut(s) 1055, 1354
RsaNI GTAC 2 cut(s) 1054, 1353
RseI CAYNNNNRTG 1 cut(s) 1620
SacI GAGCTC 2 cut(s) 51, 159
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 204, 806
SatI GCNGC 7 cut(s) 459, 771, 1095, 1143, 1434, 1584, 1671
Sau96I GGNCC 3 cut(s) 1021, 1210, 1327
SchI GAGTC 3 cut(s) 365, 716, 820
ScrFI CCNGG 5 cut(s) 184, 630, 651, 1214, 1405
SduI GDGCHC 3 cut(s) 51, 159, 625
SfaNI GCATC 4 cut(s) 466, 805, 970, 1864
SfcI CTRYAG 3 cut(s) 456, 1089, 1986
Sfr274I CTCGAG 1 cut(s) 407
SinI GGWCC 2 cut(s) 1021, 1327
SlaI CTCGAG 1 cut(s) 407
SmiMI CAYNNNNRTG 1 cut(s) 1620
SmlI CTYRAG 3 cut(s) 407, 686, 1682
SmoI CTYRAG 3 cut(s) 407, 686, 1682
Sse9I AATT 6 cut(s) 137, 438, 996, 1313, 1598, 1729
SsiI CCGC 7 cut(s) 531, 588, 983, 1024, 1193, 1489, 1793
SspMI CTAG 4 cut(s) 383, 593, 894, 1502
SstI GAGCTC 2 cut(s) 51, 159
StyD4I CCNGG 5 cut(s) 182, 628, 649, 1212, 1403
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 1 cut(s) 1007
TaiI ACGT 2 cut(s) 1226, 2237
TaqI TCGA 7 cut(s) 189, 366, 408, 485, 673, 930, 1065
TasI AATT 6 cut(s) 137, 438, 996, 1313, 1598, 1729
TatI WGTACW 1 cut(s) 1053
Tru1I TTAA 2 cut(s) 204, 806
Tru9I TTAA 2 cut(s) 204, 806
TscAI CASTG 1 cut(s) 1047
TseFI GTSAC 1 cut(s) 1038
TseI GCWGC 7 cut(s) 458, 770, 1094, 1142, 1433, 1583, 1670
Tsp45I GTSAC 1 cut(s) 1038
TspDTI ATGAA 7 cut(s) 49, 94, 150, 327, 444, 1083, 2211
TspRI CASTG 1 cut(s) 1047
VpaK11BI GGWCC 2 cut(s) 1021, 1327
XapI RAATTY 4 cut(s) 137, 438, 1313, 1729
XceI RCATGY 2 cut(s) 330, 1390
XcmI CCANNNNNNNNNTGG 2 cut(s) 1105, 1337
XhoI CTCGAG 1 cut(s) 407
XmaJI CCTAGG 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 1320
XmnI GAANNNNTTC 1 cut(s) 1081
XspI CTAG 4 cut(s) 383, 593, 894, 1502
ZraI GACGTC 1 cut(s) 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.