pycom14g15740

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011994
Physical Location & Seq
Forward (+)
18265368 .. 18266888
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g15740.1

Sequence Viewer

Length: 1155 bp
ATGTCGGACAATGAGTCCGAGCTTCCAGAGGACGAGTATGAATCACAACCACCGAATCGTGTCTCTCAGTGTGAGTTAAACGATGGAAATTGTGCAGCAGTCAAGGAAGATCCCAAAGCTGCAACGGTGCCATTGTTGACAGAAGGTGAAGATTTGGCTGATGATGCAAACAGGACTCCTCTTTTTGGTAAAGGAAACAGGTTTTTCGGAATCCCACTTACAGATTCAGCTACAAACAGTCCCAGGGTGGCAATGAGGATTTCAAGGAAATCGCCACTTGAAAAAACCGGCGAGTATGCCTTAGAATCTGTCGAGGGAAGCACTTCAAATGAGGCAGAGTGTGATTCCATTCTGCACCGGTTGAAGAGGCAGGTCCGGTTAGATAGGAAGTCGCTGATAGCATTGTACATGGAGCTAGATGAAGAAAGAAGCGCTTCGGCTGTGGCAGCGAACAATGCAATGGCTATGATCACCCGGCTACAAGCAGAGAAGGCTGCTGTTCAGATGGAAGCCTTGCAGTATCAAAGAATGATGGAGGAACAGGCGGAGTACGACCAAGAAGCACTGGAAGCGACGTATGATCTGCTTGCCAAGAGAGAGGAGGAGCTCAGACTTATAGAAGCTGAAGTAGAGGCGTATAGAGAAAAATACGGATTGCTGAAGGAATTCGCCTACGAATCTGGTGATGAGGGCAGAGGGAAAAATGACTGCGGCAGTTCTCCCGGCGAGCCTAATGGCGAAAACACACAAAATGATCAATCAAAGGAGGAGAACTTAGAGGGAGCCCTTGCTGAATCTTTGAAGCCTCTGAAGGGGGAGAAAACCTACCTCCTGGGTCGGTTGAAGAAACTCGATAAGAAAACCAACTCCGCTAAGGGGTTCTATTCTTTGCAGGGCCCTGACGGTCTGAATCATACGGATGATGAGATAGGAAATGGAAATAAAGCTGCTCTAACAAGGCAATTGTCGTTGTTGTCTCATCTTAGTGAAAGGGTGAAGGCCCTTGAGTCAGACACAGGATTCCTAGAGCATGCAGCTAAGACACTTGAGAAACATAGTGAAGGAACAAAACTTTTGACAGAGATAACTCAAAACTTACAGAAGCTTCGGCATCTCGTCATGATGCCCCCGGAGGAAAACAGCGGGTTACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

385

Amino Acids

42.63

Weight (kDa)

4.82

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 120 - 210 3.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 127
AciI CCGC 4 cut(s) 545, 711, 870, 1143
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 3 cut(s) 645, 680, 830
AfaI GTAC 2 cut(s) 407, 551
AfeI AGCGCT 1 cut(s) 433
AfiI CCNNNNNNNGG 3 cut(s) 185, 812, 876
AgeI ACCGGT 1 cut(s) 357
AgsI TTSAA 6 cut(s) 264, 281, 327, 364, 802, 844
AhdI GACNNNNNGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 242, 831
AluBI AGCT 9 cut(s) 22, 119, 230, 415, 607, 623, 947, 1037, 1105
AluI AGCT 9 cut(s) 22, 119, 230, 415, 607, 623, 947, 1037, 1105
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 2 cut(s) 67, 981
AlwI GGATC 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 433
AoxI GGCC 2 cut(s) 895, 999
ApaI GGGCCC 1 cut(s) 899
ApeKI GCWGC 6 cut(s) 95, 119, 446, 494, 947, 1034
ApoI RAATTY 1 cut(s) 665
AsiGI ACCGGT 1 cut(s) 357
Asp700I GAANNNNTTC 3 cut(s) 322, 433, 665
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 4 cut(s) 373, 895, 896, 1000
AsuC2I CCSGG 3 cut(s) 475, 723, 1130
AsuHPI GGTGA 4 cut(s) 158, 463, 695, 1006
AvaII GGWCC 1 cut(s) 373
BaeGI GKGCMC 1 cut(s) 899
BanI GGYRCC 1 cut(s) 127
BanII GRGCYC 3 cut(s) 609, 787, 899
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 6 cut(s) 106, 107, 458, 481, 934, 1046
BccI CCATC 3 cut(s) 77, 499, 526
BciT130I CCWGG 2 cut(s) 244, 833
BclI TGATCA 2 cut(s) 468, 754
BcnI CCSGG 3 cut(s) 475, 723, 1130
BcoDI GTCTC 2 cut(s) 67, 981
BfaI CTAG 2 cut(s) 416, 1025
BfoI RGCGCY 1 cut(s) 435
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 7 cut(s) 96, 120, 447, 495, 712, 948, 1035
BlsI GCNGC 7 cut(s) 97, 121, 448, 496, 713, 949, 1036
Bme1390I CCNGG 5 cut(s) 244, 475, 723, 833, 1130
Bme18I GGWCC 1 cut(s) 373
BmeRI GACNNNNNGTC 1 cut(s) 13
BmgT120I GGNCC 4 cut(s) 373, 895, 896, 1000
BmiI GGNNCC 3 cut(s) 129, 784, 897
BmrFI CCNGG 5 cut(s) 244, 475, 723, 833, 1130
BmsI GCATC 3 cut(s) 154, 1113, 1120
Bpu10I CCTNAGC 1 cut(s) 873
BpuEI CTTGAG 2 cut(s) 1025, 1067
BpuMI CCSGG 3 cut(s) 475, 723, 1130
BsaJI CCNNGG 4 cut(s) 242, 243, 832, 1128
BsaWI WCCGGW 2 cut(s) 357, 375
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 812, 876
Bse118I RCCGGY 2 cut(s) 287, 357
Bse1I ACTGG 1 cut(s) 570
Bse3DI GCAATG 2 cut(s) 258, 465
BseBI CCWGG 2 cut(s) 244, 833
BseDI CCNNGG 4 cut(s) 242, 243, 832, 1128
BseGI GGATG 1 cut(s) 925
BseLI CCNNNNNNNGG 3 cut(s) 185, 812, 876
BseMI GCAATG 2 cut(s) 258, 465
BseMII CTCAG 2 cut(s) 80, 622
BseNI ACTGG 1 cut(s) 570
BseRI GAGGAG 4 cut(s) 168, 614, 617, 782
BseSI GKGCMC 1 cut(s) 899
BseXI GCAGC 6 cut(s) 106, 107, 458, 481, 934, 1046
BsgI GTGCAG 2 cut(s) 114, 338
BshFI GGCC 2 cut(s) 897, 1001
BshNI GGYRCC 1 cut(s) 127
BshTI ACCGGT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 6 cut(s) 288, 358, 376, 475, 723, 1130
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 3 cut(s) 185, 812, 876
BsmAI GTCTC 2 cut(s) 67, 981
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 2 cut(s) 897, 1001
Bsp120I GGGCCC 1 cut(s) 895
Bsp1286I GDGCHC 3 cut(s) 609, 787, 899
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 4 cut(s) 109, 468, 580, 754
BspACI CCGC 4 cut(s) 545, 711, 870, 1143
BspANI GGCC 2 cut(s) 897, 1001
BspCNI CTCAG 2 cut(s) 79, 621
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 3 cut(s) 129, 784, 897
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 258, 465
BsrFI RCCGGY 2 cut(s) 287, 357
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 1 cut(s) 570
BssAI RCCGGY 2 cut(s) 287, 357
BssECI CCNNGG 4 cut(s) 242, 243, 832, 1128
BssMI GATC 4 cut(s) 109, 468, 580, 754
Bst2UI CCWGG 2 cut(s) 244, 833
Bst4CI ACNGT 4 cut(s) 127, 239, 905, 1152
Bst6I CTCTTC 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 3 cut(s) 588, 728, 1032
BstDEI CTNAG 7 cut(s) 66, 301, 608, 775, 873, 983, 1038
BstEII GGTNACC 1 cut(s) 1146
BstF5I GGATG 1 cut(s) 925
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 4 cut(s) 112, 471, 583, 757
BstMAI GTCTC 2 cut(s) 67, 981
BstMBI GATC 4 cut(s) 109, 468, 580, 754
BstMWI GCNNNNNNNGC 5 cut(s) 164, 446, 455, 491, 569
BstNI CCWGG 2 cut(s) 244, 833
BstNSI RCATGY 1 cut(s) 1034
BstPI GGTNACC 1 cut(s) 1146
BstSCI CCNGG 5 cut(s) 242, 473, 721, 831, 1128
BstSLI GKGCMC 1 cut(s) 899
BstV1I GCAGC 6 cut(s) 106, 107, 458, 481, 934, 1046
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 2 cut(s) 897, 1001
BtsCI GGATG 1 cut(s) 925
BtsIMutI CAGTG 2 cut(s) 74, 563
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 3 cut(s) 588, 728, 1032
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 1 cut(s) 434
Cfr10I RCCGGY 2 cut(s) 287, 357
Cfr13I GGNCC 4 cut(s) 373, 895, 896, 1000
Csp6I GTAC 2 cut(s) 406, 550
CspAI ACCGGT 1 cut(s) 357
CviAII CATG 3 cut(s) 409, 1031, 1120
CviQI GTAC 2 cut(s) 406, 550
DdeI CTNAG 7 cut(s) 66, 301, 608, 775, 873, 983, 1038
DpnI GATC 4 cut(s) 111, 470, 582, 756
DpnII GATC 4 cut(s) 109, 468, 580, 754
DriI GACNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 359
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 359
EciI GGCGGA 1 cut(s) 560
Ecl136II GAGCTC 1 cut(s) 607
Eco24I GRGCYC 3 cut(s) 609, 787, 899
Eco47I GGWCC 1 cut(s) 373
Eco47III AGCGCT 1 cut(s) 433
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 3 cut(s) 645, 680, 830
Eco91I GGTNACC 1 cut(s) 1146
EcoICRI GAGCTC 1 cut(s) 607
EcoO109I RGGNCCY 3 cut(s) 895, 896, 1000
EcoO65I GGTNACC 1 cut(s) 1146
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 242, 831
EcoT38I GRGCYC 3 cut(s) 609, 787, 899
FaeI CATG 3 cut(s) 412, 1034, 1123
FalI AAGNNNNNCTT 2 cut(s) 418, 450
FaqI GGGAC 1 cut(s) 225
FatI CATG 3 cut(s) 408, 1030, 1119
FauI CCCGC 1 cut(s) 1136
FbaI TGATCA 2 cut(s) 468, 754
Fnu4HI GCNGC 7 cut(s) 96, 120, 447, 495, 712, 948, 1035
FokI GGATG 1 cut(s) 932
FriOI GRGCYC 3 cut(s) 609, 787, 899
Fsp4HI GCNGC 7 cut(s) 96, 120, 447, 495, 712, 948, 1035
FspBI CTAG 2 cut(s) 416, 1025
GlaI GCGC 1 cut(s) 433
GluI GCNGC 7 cut(s) 96, 120, 447, 495, 712, 948, 1035
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 2 cut(s) 897, 1001
HapII CCGG 6 cut(s) 288, 358, 376, 475, 723, 1130
HhaI GCGC 1 cut(s) 434
Hin1II CATG 3 cut(s) 412, 1034, 1123
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 1103
HpaII CCGG 6 cut(s) 288, 358, 376, 475, 723, 1130
HphI GGTGA 4 cut(s) 158, 463, 695, 1006
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 8 cut(s) 7, 19, 209, 504, 611, 810, 909, 1012
Hpy188III TCNNGA 2 cut(s) 26, 1120
Hpy8I GTNNAC 1 cut(s) 138
Hpy99I CGWCG 1 cut(s) 577
HpyAV CCTTC 6 cut(s) 137, 484, 655, 805, 991, 1055
HpyCH4III ACNGT 4 cut(s) 127, 239, 905, 1152
HpyCH4IV ACGT 1 cut(s) 575
HpyCH4V TGCA 8 cut(s) 95, 122, 167, 355, 458, 517, 892, 1034
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 446, 455, 491, 569
HpyF3I CTNAG 7 cut(s) 66, 301, 608, 775, 873, 983, 1038
HpySE526I ACGT 1 cut(s) 575
Hsp92II CATG 3 cut(s) 412, 1034, 1123
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 2 cut(s) 468, 754
Kzo9I GATC 4 cut(s) 109, 468, 580, 754
LmnI GCTCC 3 cut(s) 412, 604, 782
Lsp1109I GCAGC 6 cut(s) 106, 107, 458, 481, 934, 1046
LweI GCATC 3 cut(s) 154, 1113, 1120
MaeI CTAG 2 cut(s) 416, 1025
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 1 cut(s) 1146
MalI GATC 4 cut(s) 111, 470, 582, 756
MboI GATC 4 cut(s) 109, 468, 580, 754
MboII GAAGA 5 cut(s) 119, 161, 376, 434, 856
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 3 cut(s) 609, 787, 899
MluCI AATT 3 cut(s) 88, 665, 962
MlyI GAGTC 3 cut(s) 23, 169, 1016
MroXI GAANNNNTTC 3 cut(s) 322, 433, 665
MseI TTAA 1 cut(s) 77
MslI CAYNNNNRTG 2 cut(s) 918, 984
MspA1I CMGCKG 1 cut(s) 1143
MspI CCGG 6 cut(s) 288, 358, 376, 475, 723, 1130
MspR9I CCNGG 5 cut(s) 244, 475, 723, 833, 1130
MunI CAATTG 1 cut(s) 962
MvaI CCWGG 2 cut(s) 244, 833
MwoI GCNNNNNNNGC 5 cut(s) 164, 446, 455, 491, 569
NciI CCSGG 3 cut(s) 475, 723, 1130
NdeII GATC 4 cut(s) 109, 468, 580, 754
NlaIII CATG 3 cut(s) 412, 1034, 1123
NlaIV GGNNCC 3 cut(s) 129, 784, 897
NspI RCATGY 1 cut(s) 1034
PaeI GCATGC 1 cut(s) 1034
PagI TCATGA 1 cut(s) 1119
PasI CCCWGGG 1 cut(s) 243
PdmI GAANNNNTTC 3 cut(s) 322, 433, 665
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 7 cut(s) 97, 121, 448, 496, 713, 949, 1036
PleI GAGTC 3 cut(s) 22, 169, 1015
PpsI GAGTC 3 cut(s) 22, 169, 1015
Psp124BI GAGCTC 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 242, 831
PspEI GGTNACC 1 cut(s) 1146
PspGI CCWGG 2 cut(s) 242, 831
PspN4I GGNNCC 3 cut(s) 129, 784, 897
PspOMI GGGCCC 1 cut(s) 895
PspPI GGNCC 4 cut(s) 373, 895, 896, 1000
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 2 cut(s) 407, 551
RsaNI GTAC 2 cut(s) 406, 550
RseI CAYNNNNRTG 2 cut(s) 918, 984
SacI GAGCTC 1 cut(s) 609
SaqAI TTAA 1 cut(s) 77
SatI GCNGC 7 cut(s) 96, 120, 447, 495, 712, 948, 1035
Sau3AI GATC 4 cut(s) 109, 468, 580, 754
Sau96I GGNCC 4 cut(s) 373, 895, 896, 1000
SchI GAGTC 3 cut(s) 23, 169, 1016
ScrFI CCNGG 5 cut(s) 244, 475, 723, 833, 1130
SduI GDGCHC 3 cut(s) 609, 787, 899
SfaNI GCATC 3 cut(s) 154, 1113, 1120
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 918, 984
SmlI CTYRAG 2 cut(s) 1004, 1046
SmoI CTYRAG 2 cut(s) 1004, 1046
SphI GCATGC 1 cut(s) 1034
Sse9I AATT 3 cut(s) 88, 665, 962
SsiI CCGC 4 cut(s) 545, 711, 870, 1143
SspMI CTAG 2 cut(s) 416, 1025
SstI GAGCTC 1 cut(s) 609
StyD4I CCNGG 5 cut(s) 242, 473, 721, 831, 1128
TaaI ACNGT 4 cut(s) 127, 239, 905, 1152
TaiI ACGT 1 cut(s) 578
TaqI TCGA 2 cut(s) 312, 852
TasI AATT 3 cut(s) 88, 665, 962
TatI WGTACW 1 cut(s) 405
TauI GCSGC 1 cut(s) 714
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 2 cut(s) 74, 570
TseI GCWGC 6 cut(s) 95, 119, 446, 494, 947, 1034
TspDTI ATGAA 2 cut(s) 54, 435
TspGWI ACGGA 2 cut(s) 666, 932
TspRI CASTG 2 cut(s) 74, 570
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 1 cut(s) 665
XceI RCATGY 1 cut(s) 1034
XmnI GAANNNNTTC 3 cut(s) 322, 433, 665
XspI CTAG 2 cut(s) 416, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.