Rh7CG032200

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0011994
Physical Location & Seq
Forward (+)
2233436 .. 2236048
2613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG032200.1

Sequence Viewer

Length: 2361 bp
ATGAGTTCAAACAAGAAAACAATAACAAAGGGACGCTTCACCCTTTATGTGGAGCGAAACATGGGAGAGCTCTCACGCTTCATGGTCTATGCTGTGCTTGAATGGGCCTTGATCATTCTGCTCTTCATTGATGGCTTTTTTGCTTTTGTTGCCAATGAATTCGCCAAGCTATTTGAGCTCAGATACCCCTGCTGGCTTTGCACCAGGATCGATCACATTCTCGTTAACAGAGGTCGTGATTTCTATTATAATGATTCCATATGTGAATCACACAAGAAAGATGTCTCATACCTTGCTTACTGCCACAACCACAAGAGGCTATCCGACATAAGAAAGATGTGTGAGGCATGTCTTCTTTCATTTGCAACCGAAAAAGAGTCTGATTTCGACACATACAAGTCCCTAGTTGGGATCTTGCACAAGGATCTCGAGTGCTTTGTGGAGGATGATGAACATCAAATTCAGTTGAGTGTGCCTGCAGCAAGGACATGGGAGGATGCAGGGTCGATTGAGAAGAGTAGGGACAGCATGACCATAGGGTGTTCATGTTGCGGGGCGCCATTGAAGTTGAGTTCAGCTTCCTCCTACTCAAAAGCAAAGAGTGTTAGCGGGCTAGCGCAAGCTCCTACGCCTTCTCCACGAGCACCCACAGGGAGAAATGATGATAATCCTGGTTTGGACTTGCCCCCTATTCGATATCCAGAACTCAAGTTTATGTCTGATAATGAGTCTGAGCTTCCAGAGGATGTGTATGGAACACACATGAAAAATCAAAAGGAAGACCCGAAAGCTGCTACATTGCCATTATTGACAGAAGGTGATGAGTTAGCTGATGATGCTAACAGGACTCCTATATATGGCAGAGGAAACAGGTTCTTTGGAATCCCACTTTCAGATTCAGCTCAAAATAGTCCTAGGGTGGCTATGAGGATTTCAAAGAAATCACCACTCGAAAAGAGCGAGTTTGCTTTGGAATCGGTTGATGGAAATCTTCCAAATGATGCGGATTGTGATTCAATTTTGCACCGTCTGAAGAGACAGGTCCGCTTGGACAGGAAGTCACTGATGGCTTTGTACATGGAACTCGATGAAGAAAGAAGTGCTTCAGCTGTAGCAGCCAACAATGCAATGGCTATGATCACAAGGCTACAAGCAGAGAAGGCTGCTGTTCAGATGGAGGCATTGCAATATCAGAGAATGATGGAGGAGCAGGCGGAGTATGACCAAGAAGCCCTGGAAGCAACATATGATTTGCTTGCCAAGAGAGAAGATGATCTCAGGGTTTTAGAGGCTGAAATAGAGGCTTATAGGGAGAAATATGGATTGTTGAAGGAATTTGTCTACAAGGACCAAGATGAGGCTGGTGAATGTGGTACTCCTCTAAGCGTAAAGTATGGCGCAAACAACATGCAAAATGATCAAGCCGGGTCAGCAAAGGAAGAGAATGCAGAGGCTGCCCTCGGTGAAAGTTTAAAACCACTGAAAGGGGAGAAGACTTACCTACTTGGGCGGTTCAAGAAACTAGACAAGATGAGAAACTCATCATCTAATGGGTTCTTGCCAGATGGTCTGAATGAGGATGAGATAGGAAATGGAAACAAAGCTGCCCTAACAAGGCAATTGTCGCTGTTGTCTCATCTTAGCGATAGAGTGAAGGCTCTTGAATCTGACACCGGATTCCTAGAGTATGCTGCTAAGACACTTGAGAAACATAGTGAAGGAACAAAACTTTTGACAGAGATAACTCAAAATTTACAGAAGCTCCGGCACCTAGTGAATCTGCCTACTGAGGAACAAAACAAGACGCCACCGGTGGAACAAAAGAAGGAGCAAAACAAGACGAATTCGGAGGAACAAAAGAAGGAGCAAAACAAGACGCTTCCGGAGGAACAAAATAAGACGTCTCCGGAGGAACAAAAGAAGGAGCAAAGTAAGACGCCTTCAGAGGAGCAAAACAAGACGCCTCCGGAGGAACAAAATAAGGCGCATCCGGAGGAACAAAAGGAGCAAAGTAATATGCCTTCGGAGGAACAAAAGGAGCAAAACAAGAAGCTTCCGGAGGATCAAAAGGAGCAAAGCAAGACACCTCCAGAGGAACAAAAGAAGGAGCAAAGTAAGACGCCTCCACAAGAACAAAAGAACGAGCAAAGTAAGACGCCATCGGAAGAACAAAACAAGGAGCAAAACAATATGCCCCCGGAGGAACAAAGTAAGACGCCTCCGGAGGAACAAAATGAGCAAAAGAAGACGCTTTCAGATGAACAAAATAAGATTCCTCCGGAAGAACAAAACAAGACGTCTCCAGAAGAACAGAAAAAGGAACAAAAAGACCTTACTTCTATAGTAAAAAGTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

786

Amino Acids

89.42

Weight (kDa)

5.15

Isoelectric Point (pI)

62.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 343 - 433 1.6e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AatII GACGTC 2 cut(s) 1904, 2300
AccB1I GGYRCC 2 cut(s) 556, 1767
AccI GTMKAC 1 cut(s) 1341
AccIII TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
AciI CCGC 6 cut(s) 552, 609, 1004, 1045, 1214, 1510
AclWI GGATC 4 cut(s) 215, 419, 432, 2070
AcsI RAATTY 5 cut(s) 158, 459, 1334, 1750, 1843
AcuI CTGAAG 3 cut(s) 1052, 1089, 1926
AcyI GRCGYC 9 cut(s) 557, 1805, 1901, 1937, 1961, 2120, 2156, 2216, 2297
AdeI CACNNNGTG 1 cut(s) 1774
AfaI GTAC 2 cut(s) 1076, 1375
AfiI CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
AgeI ACCGGT 1 cut(s) 1810
AgsI TTSAA 8 cut(s) 9, 101, 565, 936, 1017, 1330, 1516, 1664
AhdI GACNNNNNGTC 1 cut(s) 1057
AjnI CCWGG 3 cut(s) 203, 670, 1233
Alw21I GWGCWC 3 cut(s) 72, 180, 646
Alw26I GTCTC 5 cut(s) 289, 1030, 1638, 1908, 2304
AlwI GGATC 4 cut(s) 215, 419, 432, 2070
AlwNI CAGNNNCTG 1 cut(s) 1454
Ama87I CYCGRG 1 cut(s) 428
Aor13HI TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 7 cut(s) 479, 791, 1115, 1163, 1454, 1604, 1691
ApoI RAATTY 5 cut(s) 158, 459, 1334, 1750, 1843
AsiGI ACCGGT 1 cut(s) 1810
Asp700I GAANNNNTTC 1 cut(s) 1102
AspA2I CCTAGG 1 cut(s) 914
AspLEI GCGC 4 cut(s) 559, 619, 1400, 1987
AspS9I GGNCC 3 cut(s) 105, 1042, 1348
AsuC2I CCSGG 2 cut(s) 1426, 2198
AsuHPI GGTGA 5 cut(s) 31, 830, 936, 1376, 1475
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 1042, 1348
AvrII CCTAGG 1 cut(s) 914
BanI GGYRCC 2 cut(s) 556, 1767
BanII GRGCYC 2 cut(s) 72, 180
BarI GAAGNNNNNNTAC 4 cut(s) 1924, 1956, 2005, 2037
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 4 cut(s) 344, 786, 1499, 2252
Bbv12I GWGCWC 3 cut(s) 72, 180, 646
BbvI GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
BccI CCATC 7 cut(s) 125, 977, 1060, 1168, 1195, 1559, 2167
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 3 cut(s) 205, 672, 1235
BclI TGATCA 3 cut(s) 111, 1137, 1417
BcnI CCSGG 2 cut(s) 1426, 2198
BcoDI GTCTC 5 cut(s) 289, 1030, 1638, 1908, 2304
BfaI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
BfmI CTRYAG 3 cut(s) 477, 1110, 2340
BfoI RGCGCY 1 cut(s) 560
BisI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
BlnI CCTAGG 1 cut(s) 914
BlsI GCNGC 7 cut(s) 481, 793, 1117, 1165, 1456, 1606, 1693
Bme1390I CCNGG 5 cut(s) 205, 672, 1235, 1426, 2198
Bme18I GGWCC 2 cut(s) 1042, 1348
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 105, 1042, 1348
BmiI GGNNCC 2 cut(s) 558, 1769
BmrFI CCNGG 5 cut(s) 205, 672, 1235, 1426, 2198
BmsI GCATC 4 cut(s) 487, 826, 991, 1996
BmtI GCTAGC 1 cut(s) 617
BpiI GAAGAC 4 cut(s) 344, 786, 1499, 2252
BpmI CTGGAG 2 cut(s) 2073, 2286
BpuEI CTTGAG 2 cut(s) 692, 1724
BpuMI CCSGG 2 cut(s) 1426, 2198
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 3 cut(s) 453, 666, 987
BsaHI GRCGYC 9 cut(s) 557, 1805, 1901, 1937, 1961, 2120, 2156, 2216, 2297
BsaJI CCNNGG 4 cut(s) 914, 1233, 1459, 2196
BsaWI WCCGGW 9 cut(s) 1673, 1810, 1882, 1906, 1966, 1990, 2056, 2221, 2278
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 1746, 1776
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
Bse118I RCCGGY 1 cut(s) 1810
Bse3DI GCAATG 3 cut(s) 797, 1134, 1181
Bse8I GATNNNNATC 3 cut(s) 453, 666, 987
BseAI TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
BseBI CCWGG 3 cut(s) 205, 672, 1235
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 4 cut(s) 914, 1233, 1459, 2196
BseGI GGATG 5 cut(s) 451, 502, 751, 1585, 1987
BseJI GATNNNNATC 3 cut(s) 453, 666, 987
BseLI CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
BseMI GCAATG 3 cut(s) 797, 1134, 1181
BseMII CTCAG 4 cut(s) 193, 723, 1291, 1779
BseRI GAGGAG 3 cut(s) 1220, 1368, 1961
BseXI GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
BshFI GGCC 1 cut(s) 107
BshNI GGYRCC 2 cut(s) 556, 1767
BshTI ACCGGT 1 cut(s) 1810
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 3 cut(s) 72, 180, 646
BsiHKCI CYCGRG 1 cut(s) 428
BslFI GGGAC 3 cut(s) 45, 385, 536
BslI CCNNNNNNNGG 6 cut(s) 49, 408, 857, 1357, 1484, 1614
BsmAI GTCTC 5 cut(s) 289, 1030, 1638, 1908, 2304
BsmBI CGTCTC 2 cut(s) 1908, 2304
BsmFI GGGAC 3 cut(s) 45, 385, 536
BsmI GAATGC 1 cut(s) 1450
BsnI GGCC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 72, 180, 646
Bsp13I TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
Bsp1407I TGTACA 1 cut(s) 1074
Bsp143I GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
BspACI CCGC 6 cut(s) 552, 609, 1004, 1045, 1214, 1510
BspANI GGCC 1 cut(s) 107
BspCNI CTCAG 4 cut(s) 192, 724, 1290, 1780
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
BspLI GGNNCC 2 cut(s) 558, 1769
BspMAI CTGCAG 1 cut(s) 481
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 4 cut(s) 215, 419, 432, 2070
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 2 cut(s) 556, 1767
BsrDI GCAATG 3 cut(s) 797, 1134, 1181
BsrFI RCCGGY 1 cut(s) 1810
BsrGI TGTACA 1 cut(s) 1074
BssAI RCCGGY 1 cut(s) 1810
BssECI CCNNGG 4 cut(s) 914, 1233, 1459, 2196
BssMI GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
BssNI GRCGYC 9 cut(s) 557, 1805, 1901, 1937, 1961, 2120, 2156, 2216, 2297
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 914
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 3 cut(s) 205, 672, 1235
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 4 cut(s) 128, 509, 1028, 1434
BstACI GRCGYC 9 cut(s) 557, 1805, 1901, 1937, 1961, 2120, 2156, 2216, 2297
BstAPI GCANNNNNTGC 1 cut(s) 1454
BstAUI TGTACA 1 cut(s) 1074
BstC8I GCNNGC 7 cut(s) 194, 477, 611, 615, 621, 1212, 1257
BstDEI CTNAG 7 cut(s) 179, 732, 1277, 1382, 1640, 1695, 1788
BstF5I GGATG 5 cut(s) 451, 502, 751, 1585, 1987
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 4 cut(s) 559, 619, 1400, 1987
BstKTI GATC 9 cut(s) 114, 210, 214, 414, 427, 1140, 1276, 1420, 2065
BstMAI GTCTC 5 cut(s) 289, 1030, 1638, 1908, 2304
BstMBI GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
BstNI CCWGG 3 cut(s) 205, 672, 1235
BstNSI RCATGY 2 cut(s) 351, 1411
BstSCI CCNGG 5 cut(s) 203, 670, 1233, 1424, 2196
BstSFI CTRYAG 3 cut(s) 477, 1110, 2340
BstV1I GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
BstV2I GAAGAC 4 cut(s) 344, 786, 1499, 2252
BstX2I RGATCY 2 cut(s) 411, 424
BstYI RGATCY 2 cut(s) 411, 424
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 1 cut(s) 107
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 5 cut(s) 451, 502, 751, 1585, 1987
BtsIMutI CAGTG 2 cut(s) 1061, 1478
Cac8I GCNNGC 7 cut(s) 194, 477, 611, 615, 621, 1212, 1257
CaiI CAGNNNCTG 1 cut(s) 1454
CfoI GCGC 4 cut(s) 559, 619, 1400, 1987
Cfr10I RCCGGY 1 cut(s) 1810
Cfr13I GGNCC 3 cut(s) 105, 1042, 1348
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 9 cut(s) 42, 1813, 1885, 1945, 1969, 2128, 2164, 2224, 2257
Csp6I GTAC 2 cut(s) 1075, 1374
CspAI ACCGGT 1 cut(s) 1810
CviAII CATG 9 cut(s) 61, 82, 348, 489, 529, 546, 763, 1078, 1408
CviQI GTAC 2 cut(s) 1075, 1374
DdeI CTNAG 7 cut(s) 179, 732, 1277, 1382, 1640, 1695, 1788
DinI GGCGCC 1 cut(s) 558
DpnI GATC 9 cut(s) 113, 209, 213, 413, 426, 1139, 1275, 1419, 2064
DpnII GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
DraI TTTAAA 1 cut(s) 1473
DraIII CACNNNGTG 1 cut(s) 1774
DriI GACNNNNNGTC 1 cut(s) 1057
Eam1104I CTCTTC 4 cut(s) 128, 509, 1028, 1434
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 4 cut(s) 128, 509, 1028, 1434
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 2 cut(s) 70, 178
Eco130I CCWWGG 1 cut(s) 914
Eco24I GRGCYC 2 cut(s) 72, 180
Eco32I GATATC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 1042, 1348
Eco53kI GAGCTC 2 cut(s) 70, 178
Eco57I CTGAAG 3 cut(s) 1052, 1089, 1926
Eco88I CYCGRG 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 70, 178
EcoRI GAATTC 2 cut(s) 158, 1843
EcoRII CCWGG 3 cut(s) 203, 670, 1233
EcoRV GATATC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 914
EcoT38I GRGCYC 2 cut(s) 72, 180
EgeI GGCGCC 1 cut(s) 558
EheI GGCGCC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 914
Esp3I CGTCTC 2 cut(s) 1908, 2304
FaeI CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 1087, 1119
FaqI GGGAC 3 cut(s) 45, 385, 536
FatI CATG 9 cut(s) 60, 81, 347, 488, 528, 545, 762, 1077, 1407
FauI CCCGC 2 cut(s) 545, 602
FauNDI CATATG 2 cut(s) 260, 1246
FbaI TGATCA 3 cut(s) 111, 1137, 1417
FblI GTMKAC 1 cut(s) 1341
Fnu4HI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
FokI GGATG 5 cut(s) 458, 509, 758, 1592, 1974
FriOI GRGCYC 2 cut(s) 72, 180
Fsp4HI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
FspBI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
GlaI GCGC 4 cut(s) 558, 618, 1399, 1986
GluI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
GsuI CTGGAG 2 cut(s) 2073, 2286
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 1 cut(s) 107
HgaI GACGC 9 cut(s) 42, 1813, 1885, 1945, 1969, 2128, 2164, 2224, 2257
HhaI GCGC 4 cut(s) 559, 619, 1400, 1987
Hin1I GRCGYC 9 cut(s) 557, 1805, 1901, 1937, 1961, 2120, 2156, 2216, 2297
Hin1II CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
Hin6I GCGC 4 cut(s) 557, 617, 1398, 1985
HinP1I GCGC 4 cut(s) 557, 617, 1398, 1985
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 2051
HpaI GTTAAC 1 cut(s) 226
HphI GGTGA 5 cut(s) 31, 830, 936, 1376, 1475
Hpy166II GTNNAC 2 cut(s) 226, 1342
Hpy8I GTNNAC 2 cut(s) 226, 1342
HpyCH4III ACNGT 1 cut(s) 1028
HpyCH4IV ACGT 2 cut(s) 1901, 2297
HpyF3I CTNAG 7 cut(s) 179, 732, 1277, 1382, 1640, 1695, 1788
HpySE526I ACGT 2 cut(s) 1901, 2297
Hsp92I GRCGYC 9 cut(s) 557, 1805, 1901, 1937, 1961, 2120, 2156, 2216, 2297
Hsp92II CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
HspAI GCGC 4 cut(s) 557, 617, 1398, 1985
KasI GGCGCC 1 cut(s) 556
Kpn2I TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
Ksp22I TGATCA 3 cut(s) 111, 1137, 1417
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
LguI GCTCTTC 1 cut(s) 128
Lsp1109I GCAGC 7 cut(s) 491, 778, 1127, 1150, 1441, 1591, 1678
LweI GCATC 4 cut(s) 487, 826, 991, 1996
MaeI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
MaeII ACGT 2 cut(s) 1901, 2297
MaeIII GTNAC 1 cut(s) 1059
MalI GATC 9 cut(s) 113, 209, 213, 413, 426, 1139, 1275, 1419, 2064
MboI GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
MfeI CAATTG 1 cut(s) 1619
MflI RGATCY 2 cut(s) 411, 424
MhlI GDGCHC 3 cut(s) 72, 180, 646
MluCI AATT 7 cut(s) 158, 459, 1017, 1334, 1619, 1750, 1843
Mly113I GGCGCC 1 cut(s) 557
MlyI GAGTC 3 cut(s) 386, 737, 841
MmeI TCCRAC 1 cut(s) 348
MroI TCCGGA 7 cut(s) 1882, 1906, 1966, 1990, 2056, 2221, 2278
MroXI GAANNNNTTC 1 cut(s) 1102
MseI TTAA 2 cut(s) 225, 1472
MspA1I CMGCKG 1 cut(s) 1109
MspR9I CCNGG 5 cut(s) 205, 672, 1235, 1426, 2198
MunI CAATTG 1 cut(s) 1619
Mva1269I GAATGC 1 cut(s) 1450
MvaI CCWGG 3 cut(s) 205, 672, 1235
NarI GGCGCC 1 cut(s) 557
NciI CCSGG 2 cut(s) 1426, 2198
NdeI CATATG 2 cut(s) 260, 1246
NdeII GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 9 cut(s) 64, 85, 351, 492, 532, 549, 766, 1081, 1411
NlaIV GGNNCC 2 cut(s) 558, 1769
NmuCI GTSAC 1 cut(s) 1059
NspI RCATGY 2 cut(s) 351, 1411
PaeR7I CTCGAG 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PctI GAATGC 1 cut(s) 1450
PdmI GAANNNNTTC 1 cut(s) 1102
PinAI ACCGGT 1 cut(s) 1810
PkrI GCNGC 7 cut(s) 481, 793, 1117, 1165, 1456, 1606, 1693
PleI GAGTC 3 cut(s) 385, 736, 841
PluTI GGCGCC 1 cut(s) 560
PpsI GAGTC 3 cut(s) 385, 736, 841
PsiI TTATAA 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 72, 180
Psp6I CCWGG 3 cut(s) 203, 670, 1233
PspGI CCWGG 3 cut(s) 203, 670, 1233
PspN4I GGNNCC 2 cut(s) 558, 1769
PspPI GGNCC 3 cut(s) 105, 1042, 1348
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 1454
PsuI RGATCY 2 cut(s) 411, 424
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 2 cut(s) 1076, 1375
RsaNI GTAC 2 cut(s) 1075, 1374
SacI GAGCTC 2 cut(s) 72, 180
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 225, 1472
SatI GCNGC 7 cut(s) 480, 792, 1116, 1164, 1455, 1605, 1692
Sau3AI GATC 9 cut(s) 111, 207, 211, 411, 424, 1137, 1273, 1417, 2062
Sau96I GGNCC 3 cut(s) 105, 1042, 1348
SchI GAGTC 3 cut(s) 386, 737, 841
ScrFI CCNGG 5 cut(s) 205, 672, 1235, 1426, 2198
SduI GDGCHC 3 cut(s) 72, 180, 646
SfaNI GCATC 4 cut(s) 487, 826, 991, 1996
SfcI CTRYAG 3 cut(s) 477, 1110, 2340
SfoI GGCGCC 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 428
SgrAI CRCCGGYG 1 cut(s) 1810
SinI GGWCC 2 cut(s) 1042, 1348
SlaI CTCGAG 1 cut(s) 428
SmlI CTYRAG 3 cut(s) 428, 707, 1703
SmoI CTYRAG 3 cut(s) 428, 707, 1703
Sse9I AATT 7 cut(s) 158, 459, 1017, 1334, 1619, 1750, 1843
SsiI CCGC 6 cut(s) 552, 609, 1004, 1045, 1214, 1510
SspDI GGCGCC 1 cut(s) 556
SspMI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
SstI GAGCTC 2 cut(s) 72, 180
StyD4I CCNGG 5 cut(s) 203, 670, 1233, 1424, 2196
StyI CCWWGG 1 cut(s) 914
TaaI ACNGT 1 cut(s) 1028
TaiI ACGT 2 cut(s) 1904, 2300
TaqI TCGA 7 cut(s) 210, 387, 429, 506, 694, 951, 1086
TasI AATT 7 cut(s) 158, 459, 1017, 1334, 1619, 1750, 1843
TatI WGTACW 1 cut(s) 1074
Tru1I TTAA 2 cut(s) 225, 1472
Tru9I TTAA 2 cut(s) 225, 1472
TscAI CASTG 2 cut(s) 1068, 1485
TseFI GTSAC 1 cut(s) 1059
TseI GCWGC 7 cut(s) 479, 791, 1115, 1163, 1454, 1604, 1691
Tsp45I GTSAC 1 cut(s) 1059
TspDTI ATGAA 9 cut(s) 70, 115, 171, 348, 465, 534, 779, 1104, 2274
TspRI CASTG 2 cut(s) 1068, 1485
VpaK11BI GGWCC 2 cut(s) 1042, 1348
XapI RAATTY 5 cut(s) 158, 459, 1334, 1750, 1843
XceI RCATGY 2 cut(s) 351, 1411
XcmI CCANNNNNNNNNTGG 2 cut(s) 1126, 1358
XhoI CTCGAG 1 cut(s) 428
XmaJI CCTAGG 1 cut(s) 914
XmiI GTMKAC 1 cut(s) 1341
XmnI GAANNNNTTC 1 cut(s) 1102
XspI CTAG 6 cut(s) 404, 614, 915, 1523, 1682, 1772
ZraI GACGTC 2 cut(s) 1902, 2298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.