Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008647

Physical Location & Seq

Genomic Coordinates Reverse (-)

6990642 .. 6993989

Gene Structure 3348 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G20160.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGAAAGGCATTACATTCTTCACACCCTTTTTATCATTTCTATATCTCTTATGCATCTTGTTTATGACAGAAACTGAAGCTGGGTCGAGAAATGGTGATGGGGTCTACATTGTCTACATGGGATCAGCTTCCTCTGCTGCAAACGCTAATAGAGCTCAAATACTCATAAACACCATGTTTAAAAGGAGAGCAAACGATCTTCTCCACACATATAAGCATGGCTTCTCAGGTTTTGCAGCTCGTTTGACAGCAGAAGAGGCCAAGGTCATAGCCAAGAAACCGGGAGTGGTTTCAGTTTTTCCTGACCCACACTTCCAGCTTCATACAACTCATTCATGGGACTTTCTCAAGTACCAAACATCAGTAAAGGTCGATTCCGGTCCACCTTCATCAGCCTCAGATGGATCATATGATAGCATTGTCGGAATTCTTGACACAGGGATATGGCCAGAGTCAGAGAGTTTCAATGACAAAGACATGGGTCCAATTCCGTCTCGGTGGAAAGGTACATGCATGGAAGCAAAGGACTTCAAGTCTTCCAACTGTAACAGAAAGATCATTGGAGCAAGATACTACAAAAATCCTGATGATGATTCAGAATACTATACCACAAGGGATGTCATCGGTCACGGTTCTCATGTGTCCTCCACCATAGCTGGATCTGCCGTGGAGAATGCTTCCTACTATGGTGTAGCTTCCGGGACTGCAAAGGGAGGTTCACAAAACGCTAGAATCGCTATGTACAAAGTATGCAATCCAGGGGGATGCACTGGCTCCTCTATCTTAGCTGCTTTCGATGATGCAATCGCAGATGGAGTTGATGTTCTATCTCTGTCTCTTGGAGCTCCAGCATACGCTCGCATCGACTTGAACACTGATCCTATTGCCATTGGAGCGTTTCACGCGGTGGAGCAAGGAATCTTGGTGATCTGCTCTGCGGGTAATGATGGACCTGATGGCGGTACAGTTACTAATACTGCACCTTGGATAATGACCGTTGCTGCCAACACTATTGATAGAGACTTTGAGTCTGATGTTGTACTAGGCGGCAATAAAGTCATCAAGGGTGAAGGTATACACTTTTCAAACGTTAGTAAATCTCCTGTGTATCCTCTGATTCATGGCAAGTCTGCTAAGAGCGCTGATGCATCAGAAGGATCAGCCAGGGCCTGTGACTCTGATTCTCTAGATCAAGAGAAGGTAAAAGGGAAGATTGTGTTATGCGAGAACGTTGGTGGATCATATTATGCATCATCCGCTAGGGACGAGGTGAAGAGCAAAGGAGGTACTGGTTGCGTCTTTGTAGATGACAGAACTAGAGCAGTTGCTAGTGCTTATGGTAGCTTTCCTACTACCGTAATTGACTCAAAGGAAGCAGCTGAGATCTTCTCCTACCTCAACTCAACCAAAGATCCTGTTGCAACAATTCTTCCCACTGCAACAGTTGAAAAGTTCACACCTGCCCCTGCTGTTGCATATTTTTCTTCCAGAGGACCTTCAAGCCTCACAAGAAGCATTCTCAAACCTGACATTACCGCACCAGGAGTCTCGATACTCGCTGCATGGACTGGAAACGACTCAAGCATTTCACTGGAAGGCAAGCCGGCTTCTCAGTATAACGTCATATCAGGAACTTCCATGGCAGCTCCTCATGTTTCAGCTGTTGCATCTCTGATCAAATCACAGCATCCCACATGGGGTCCATCCGCGATCAGATCAGCAATTATGACAACAGCGACTCAAACAAACAACGACAAAGGTCTTATAACAACAGAAACTGGTGCAACAGCCACACCTTATGACTCTGGAGCAGGAGAACTAAGCTCAACAGCATCAATGCAACCAGGACTAGTTTACGAGACTACTGAAACTGACTACCTGAACTTTCTCTGTTACTATGGATATAACGTAACCACAATAAAGGCTATGTCAAAAGCTTTTCCAGAGAATTTTACTTGCCCTGCAGATTCCAACTTAGACTTGATCTCCACCATCAATTACCCGTCAATTGGAATCTCTGGATTCAAAGGAAATGGTAGCAAGACAGTTACAAGAACAGTGACCAATGTTGGTGAGGACGGTGAGGCGGTTTACACAGTCAGCGTGGAAACACCACCAGGGTTTAACATTCAAGTGACGCCAGAAAAACTCCAATTTACAAAAGATGGTGAGAAGTTGACATACCAGGTGATAGTGTCTGCTACTGCTTCACTTAAGCAAGATGTATTTGGGGCTCTTACTTGGTCTAATGCCAAGTACAAGGTCAGAAGCCCAATTGTAATTAGTAGCGAGAGTAGCCGCACAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0010033 GO:0010037 GO:0016787 GO:0017171 GO:0019538 GO:0030312 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0042221 GO:0042493 GO:0043170 GO:0043900 GO:0043901 GO:0044238 GO:0044464 GO:0048046 GO:0048519 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0065007 GO:0070011 GO:0071704 GO:0071944 GO:0072347 GO:0080134 GO:0140096 GO:1900424 GO:1900425 GO:1901564 GO:1901700 GO:2000026 GO:2000038 GO:2000122

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

769

Amino Acids

81.48

Weight (kDa)

5.66

Isoelectric Point (pI)

27.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	35 - 108	8e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	140 - 501	7.8e-27	Subtilase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008647)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20150 AT1G20160 AT1G20160 AT1G20160
fragaria_vesca	FvH4_2g38090
malus_domestica	MD08G1009400.v1.1
prunus_persica	Prupe.1G361300_v2.0.a1
pyrus_communis	pycom08g00790 pycom15g00680 pycom15g00690
rosa_chinensis	RchiOBHm_Chr3g0451701 RchiOBHm_Chr6g0303461
rosa_laevigata	RLG00000011082
rosa_multiflora	Rmu_ssc0000114.1_g000050
rosa_roxburghii	Rroxscaffold_7G00164720 Rroxscaffold_7G00164730
rosa_rugosa	Rorug06G0321400 Rorug06G0321500
rosa_samantha	Rh6AG436000 Rh6BG467600 Rh6CG447900 Rh6DG434600
rosa_wichuraiana	Rw6G037730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1766
AarI	CACCTGC	1 cut(s)	1468
Acc36I	ACCTGC	1 cut(s)	1468
AccI	GTMKAC	3 cut(s)	105, 114, 1075
AccII	CGCG	2 cut(s)	905, 1709
AciI	CCGC	9 cut(s)	905, 938, 960, 1047, 1257, 1536, 1707, 2087, 2299
AclI	AACGTT	2 cut(s)	1089, 1230
AclWI	GGATC	7 cut(s)	130, 412, 667, 872, 1165, 1246, 1406
AcoI	YGGCCR	1 cut(s)	446
AcsI	RAATTY	2 cut(s)	426, 1948
AcuI	CTGAAG	1 cut(s)	96
AcyI	GRCGYC	1 cut(s)	2138
AdeI	CACNNNGTG	1 cut(s)	907
AfaI	GTAC	7 cut(s)	353, 508, 743, 964, 1041, 1288, 2258
AfeI	AGCGCT	1 cut(s)	1141
AfiI	CCNNNNNNNGG	3 cut(s)	959, 1697, 2009
AflII	CTTAAG	1 cut(s)	2213
AgsI	TTSAA	8 cut(s)	466, 532, 871, 1086, 1448, 1500, 2026, 2132
AhdI	GACNNNNNGTC	2 cut(s)	532, 1027
AhlI	ACTAGT	1 cut(s)	1849
AjnI	CCWGG	6 cut(s)	757, 1163, 1540, 1843, 2116, 2184
AloI	GAACNNNNNNTCC	2 cut(s)	807, 839
Alw21I	GWGCWC	2 cut(s)	157, 847
Alw26I	GTCTC	5 cut(s)	498, 840, 1014, 1552, 1853
AlwI	GGATC	7 cut(s)	130, 412, 667, 872, 1165, 1246, 1406
AlwNI	CAGNNNCTG	3 cut(s)	74, 1170, 1778
Aor51HI	AGCGCT	1 cut(s)	1141
AoxI	GGCC	3 cut(s)	258, 446, 1167
ApeKI	GCWGC	7 cut(s)	137, 236, 788, 1001, 1376, 1559, 1643
ApoI	RAATTY	2 cut(s)	426, 1948
AspLEI	GCGC	1 cut(s)	1142
AspS9I	GGNCC	6 cut(s)	380, 482, 950, 1167, 1493, 1700
AsuC2I	CCSGG	2 cut(s)	282, 700
AsuHPI	GGTGA	8 cut(s)	107, 937, 1079, 1282, 2084, 2093, 2180, 2200
AvaII	GGWCC	5 cut(s)	380, 482, 950, 1493, 1700
BalI	TGGCCA	1 cut(s)	448
BanII	GRGCYC	3 cut(s)	157, 847, 2236
BarI	GAAGNNNNNNTAC	2 cut(s)	2165, 2197
BbsI	GAAGAC	1 cut(s)	528
Bbv12I	GWGCWC	2 cut(s)	157, 847
BbvI	GCAGC	7 cut(s)	124, 248, 775, 988, 1388, 1546, 1655
BccI	CCATC	8 cut(s)	92, 395, 806, 941, 950, 1711, 2000, 2159
BceAI	ACGGC	1 cut(s)	650
BciT130I	CCWGG	6 cut(s)	759, 1165, 1542, 1845, 2118, 2186
BciVI	GTATCC	1 cut(s)	1119
BclI	TGATCA	1 cut(s)	1674
BcnI	CCSGG	2 cut(s)	282, 700
BcoDI	GTCTC	5 cut(s)	498, 840, 1014, 1552, 1853
BcuI	ACTAGT	1 cut(s)	1849
BfaI	CTAG	7 cut(s)	729, 1043, 1187, 1260, 1317, 1329, 1850
BfmI	CTRYAG	1 cut(s)	1962
BfoI	RGCGCY	1 cut(s)	1143
BfrI	CTTAAG	1 cut(s)	2213
BfuAI	ACCTGC	1 cut(s)	1468
BfuI	GTATCC	1 cut(s)	1119
BglII	AGATCT	1 cut(s)	1383
BisI	GCNGC	9 cut(s)	138, 237, 789, 1002, 1048, 1377, 1560, 1644, 2299
BlsI	GCNGC	9 cut(s)	139, 238, 790, 1003, 1049, 1378, 1561, 1645, 2300
Bme1390I	CCNGG	8 cut(s)	282, 700, 759, 1165, 1542, 1845, 2118, 2186
Bme18I	GGWCC	5 cut(s)	380, 482, 950, 1493, 1700
BmeRI	GACNNNNNGTC	2 cut(s)	532, 1027
BmgT120I	GGNCC	6 cut(s)	380, 482, 950, 1167, 1493, 1700
BmiI	GGNNCC	3 cut(s)	483, 775, 1701
BmrFI	CCNGG	8 cut(s)	282, 700, 759, 1165, 1542, 1845, 2118, 2186
BoxI	GACNNNNGTC	2 cut(s)	480, 1758
BpiI	GAAGAC	1 cut(s)	528
BplI	GAGNNNNNCTC	2 cut(s)	1373, 1405
BpmI	CTGGAG	2 cut(s)	831, 1827
BpuEI	CTTGAG	2 cut(s)	332, 1564
BpuMI	CCSGG	2 cut(s)	282, 700
BsaHI	GRCGYC	1 cut(s)	2138
BsaJI	CCNNGG	7 cut(s)	261, 666, 758, 983, 1164, 1638, 2117
BsaWI	WCCGGW	1 cut(s)	377
BsaXI	ACNNNNNCTCC	4 cut(s)	807, 837, 1970, 2000
Bsc4I	CCNNNNNNNGG	3 cut(s)	959, 1697, 2009
Bse118I	RCCGGY	1 cut(s)	1603
Bse1I	ACTGG	5 cut(s)	775, 1294, 1573, 1596, 1783
BseBI	CCWGG	6 cut(s)	759, 1165, 1542, 1845, 2118, 2186
BseDI	CCNNGG	7 cut(s)	261, 666, 758, 983, 1164, 1638, 2117
BseGI	GGATG	5 cut(s)	622, 770, 1253, 1687, 1703
BseLI	CCNNNNNNNGG	3 cut(s)	959, 1697, 2009
BseMII	CTCAG	4 cut(s)	240, 411, 1371, 1625
BseNI	ACTGG	5 cut(s)	775, 1294, 1573, 1596, 1783
BseRI	GAGGAG	2 cut(s)	766, 1638
BseXI	GCAGC	7 cut(s)	124, 248, 775, 988, 1388, 1546, 1655
BseYI	CCCAGC	1 cut(s)	80
BsgI	GTGCAG	1 cut(s)	963
Bsh1236I	CGCG	2 cut(s)	905, 1709
BshFI	GGCC	3 cut(s)	260, 448, 1169
BsiHKAI	GWGCWC	2 cut(s)	157, 847
BsiSI	CCGG	4 cut(s)	281, 378, 699, 1604
BslFI	GGGAC	3 cut(s)	353, 715, 1277
BslI	CCNNNNNNNGG	3 cut(s)	959, 1697, 2009
BsmAI	GTCTC	5 cut(s)	498, 840, 1014, 1552, 1853
BsmBI	CGTCTC	1 cut(s)	498
BsmFI	GGGAC	3 cut(s)	353, 715, 1277
BsmI	GAATGC	2 cut(s)	679, 1515
BsnI	GGCC	3 cut(s)	260, 448, 1169
Bsp1286I	GDGCHC	3 cut(s)	157, 847, 2236
Bsp1407I	TGTACA	1 cut(s)	741
Bsp19I	CCATGG	1 cut(s)	1638
BspACI	CCGC	9 cut(s)	905, 938, 960, 1047, 1257, 1536, 1707, 2087, 2299
BspANI	GGCC	3 cut(s)	260, 448, 1169
BspCNI	CTCAG	4 cut(s)	239, 410, 1372, 1624
BspFNI	CGCG	2 cut(s)	905, 1709
BspLI	GGNNCC	3 cut(s)	483, 775, 1701
BspMAI	CTGCAG	1 cut(s)	1966
BspMI	ACCTGC	1 cut(s)	1468
BspPI	GGATC	7 cut(s)	130, 412, 667, 872, 1165, 1246, 1406
BspQI	GCTCTTC	1 cut(s)	1268
BspTI	CTTAAG	1 cut(s)	2213
BsrFI	RCCGGY	1 cut(s)	1603
BsrGI	TGTACA	1 cut(s)	741
BsrI	ACTGG	5 cut(s)	775, 1294, 1573, 1596, 1783
BssAI	RCCGGY	1 cut(s)	1603
BssECI	CCNNGG	7 cut(s)	261, 666, 758, 983, 1164, 1638, 2117
BssNAI	GTATAC	1 cut(s)	1076
BssNI	GRCGYC	1 cut(s)	2138
BssT1I	CCWWGG	3 cut(s)	261, 983, 1638
Bst1107I	GTATAC	1 cut(s)	1076
Bst2UI	CCWGG	6 cut(s)	759, 1165, 1542, 1845, 2118, 2186
Bst6I	CTCTTC	2 cut(s)	249, 1268
BstACI	GRCGYC	1 cut(s)	2138
BstAFI	CTTAAG	1 cut(s)	2213
BstAUI	TGTACA	1 cut(s)	741
BstC8I	GCNNGC	3 cut(s)	859, 1601, 1605
BstDEI	CTNAG	8 cut(s)	226, 397, 784, 1134, 1380, 1611, 1820, 1975
BstDSI	CCRYGG	2 cut(s)	666, 1638
BstF5I	GGATG	5 cut(s)	622, 770, 1253, 1687, 1703
BstFNI	CGCG	2 cut(s)	905, 1709
BstH2I	RGCGCY	1 cut(s)	1143
BstHHI	GCGC	1 cut(s)	1142
BstMAI	GTCTC	5 cut(s)	498, 840, 1014, 1552, 1853
BstNI	CCWGG	6 cut(s)	759, 1165, 1542, 1845, 2118, 2186
BstNSI	RCATGY	1 cut(s)	513
BstPAI	GACNNNNGTC	2 cut(s)	480, 1758
BstSCI	CCNGG	8 cut(s)	280, 698, 757, 1163, 1540, 1843, 2116, 2184
BstSFI	CTRYAG	1 cut(s)	1962
BstUI	CGCG	2 cut(s)	905, 1709
BstV1I	GCAGC	7 cut(s)	124, 248, 775, 988, 1388, 1546, 1655
BstV2I	GAAGAC	1 cut(s)	528
BstX2I	RGATCY	3 cut(s)	659, 1383, 1411
BstYI	RGATCY	3 cut(s)	659, 1383, 1411
BstZ17I	GTATAC	1 cut(s)	1076
BsuI	GTATCC	1 cut(s)	1119
BsuRI	GGCC	3 cut(s)	260, 448, 1169
BtgI	CCRYGG	2 cut(s)	666, 1638
BtsCI	GGATG	5 cut(s)	622, 770, 1253, 1687, 1703
BtsI	GCAGTG	1 cut(s)	1434
BtsIMutI	CAGTG	5 cut(s)	768, 873, 1434, 1589, 2064
BveI	ACCTGC	1 cut(s)	1468
Cac8I	GCNNGC	3 cut(s)	859, 1601, 1605
CaiI	CAGNNNCTG	3 cut(s)	74, 1170, 1778
CfoI	GCGC	1 cut(s)	1142
Cfr10I	RCCGGY	1 cut(s)	1603
Cfr13I	GGNCC	6 cut(s)	380, 482, 950, 1167, 1493, 1700
CseI	GACGC	2 cut(s)	1285, 2146
CsiI	ACCWGGT	1 cut(s)	2184
Csp6I	GTAC	7 cut(s)	352, 507, 742, 963, 1040, 1287, 2257
CviQI	GTAC	7 cut(s)	352, 507, 742, 963, 1040, 1287, 2257
DdeI	CTNAG	8 cut(s)	226, 397, 784, 1134, 1380, 1611, 1820, 1975
DraI	TTTAAA	1 cut(s)	181
DraIII	CACNNNGTG	1 cut(s)	907
DriI	GACNNNNNGTC	2 cut(s)	532, 1027
EaeI	YGGCCR	1 cut(s)	446
Eam1104I	CTCTTC	2 cut(s)	249, 1268
Eam1105I	GACNNNNNGTC	2 cut(s)	532, 1027
EarI	CTCTTC	2 cut(s)	249, 1268
Ecl136II	GAGCTC	2 cut(s)	155, 845
Eco130I	CCWWGG	3 cut(s)	261, 983, 1638
Eco24I	GRGCYC	3 cut(s)	157, 847, 2236
Eco47I	GGWCC	5 cut(s)	380, 482, 950, 1493, 1700
Eco47III	AGCGCT	1 cut(s)	1141
Eco53kI	GAGCTC	2 cut(s)	155, 845
Eco57I	CTGAAG	1 cut(s)	96
EcoICRI	GAGCTC	2 cut(s)	155, 845
EcoO109I	RGGNCCY	2 cut(s)	1167, 1493
EcoRI	GAATTC	1 cut(s)	426
EcoRII	CCWGG	6 cut(s)	757, 1163, 1540, 1843, 2116, 2184
EcoT14I	CCWWGG	3 cut(s)	261, 983, 1638
EcoT22I	ATGCAT	4 cut(s)	56, 515, 1150, 1252
EcoT38I	GRGCYC	3 cut(s)	157, 847, 2236
ErhI	CCWWGG	3 cut(s)	261, 983, 1638
Esp3I	CGTCTC	1 cut(s)	498
FalI	AAGNNNNNCTT	2 cut(s)	206, 238
FaqI	GGGAC	3 cut(s)	353, 715, 1277
FauI	CCCGC	1 cut(s)	931
FauNDI	CATATG	1 cut(s)	409
FbaI	TGATCA	1 cut(s)	1674
FblI	GTMKAC	3 cut(s)	105, 114, 1075
Fnu4HI	GCNGC	9 cut(s)	138, 237, 789, 1002, 1048, 1377, 1560, 1644, 2299
FokI	GGATG	5 cut(s)	629, 777, 1240, 1674, 1690
FriOI	GRGCYC	3 cut(s)	157, 847, 2236
Fsp4HI	GCNGC	9 cut(s)	138, 237, 789, 1002, 1048, 1377, 1560, 1644, 2299
FspBI	CTAG	7 cut(s)	729, 1043, 1187, 1260, 1317, 1329, 1850
GlaI	GCGC	1 cut(s)	1141
GluI	GCNGC	9 cut(s)	138, 237, 789, 1002, 1048, 1377, 1560, 1644, 2299
GsaI	CCCAGC	1 cut(s)	84
GsuI	CTGGAG	2 cut(s)	831, 1827
HaeII	RGCGCY	1 cut(s)	1143
HaeIII	GGCC	3 cut(s)	260, 448, 1169
HapII	CCGG	4 cut(s)	281, 378, 699, 1604
HgaI	GACGC	2 cut(s)	1285, 2146
HhaI	GCGC	1 cut(s)	1142
Hin1I	GRCGYC	1 cut(s)	2138
Hin6I	GCGC	1 cut(s)	1140
HinP1I	GCGC	1 cut(s)	1140
HincII	GTYRAC	1 cut(s)	2178
HindII	GTYRAC	1 cut(s)	2178
HindIII	AAGCTT	1 cut(s)	1935
HpaII	CCGG	4 cut(s)	281, 378, 699, 1604
HphI	GGTGA	8 cut(s)	107, 937, 1079, 1282, 2084, 2093, 2180, 2200
Hpy166II	GTNNAC	9 cut(s)	106, 115, 383, 719, 1076, 1455, 1855, 2092, 2178
Hpy8I	GTNNAC	9 cut(s)	106, 115, 383, 719, 1076, 1455, 1855, 2092, 2178
HpyAV	CCTTC	6 cut(s)	396, 1064, 1148, 1192, 1506, 1589
HpyCH4IV	ACGT	4 cut(s)	1089, 1230, 1620, 1908
HpyF3I	CTNAG	8 cut(s)	226, 397, 784, 1134, 1380, 1611, 1820, 1975
HpySE526I	ACGT	4 cut(s)	1089, 1230, 1620, 1908
Hsp92I	GRCGYC	1 cut(s)	2138
HspAI	GCGC	1 cut(s)	1140
KroI	GCCGGC	1 cut(s)	1603
KroNI	GCCGGC	1 cut(s)	1605
Ksp22I	TGATCA	1 cut(s)	1674
LguI	GCTCTTC	1 cut(s)	1268
LmnI	GCTCC	8 cut(s)	563, 779, 842, 850, 893, 910, 1651, 1808
Lsp1109I	GCAGC	7 cut(s)	124, 248, 775, 988, 1388, 1546, 1655
MabI	ACCWGGT	1 cut(s)	2184
MaeI	CTAG	7 cut(s)	729, 1043, 1187, 1260, 1317, 1329, 1850
MaeII	ACGT	4 cut(s)	1089, 1230, 1620, 1908
MaeIII	GTNAC	9 cut(s)	545, 626, 967, 1172, 1892, 1909, 2047, 2059, 2134
MboII	GAAGA	9 cut(s)	10, 191, 266, 528, 1222, 1285, 1378, 1421, 1476
MfeI	CAATTG	2 cut(s)	2007, 2274
MflI	RGATCY	3 cut(s)	659, 1383, 1411
MhlI	GDGCHC	3 cut(s)	157, 847, 2236
MlsI	TGGCCA	1 cut(s)	448
MluNI	TGGCCA	1 cut(s)	448
MlyI	GAGTC	8 cut(s)	461, 1037, 1169, 1358, 1554, 1571, 1732, 1796
MmeI	TCCRAC	3 cut(s)	403, 564, 1995
Mox20I	TGGCCA	1 cut(s)	448
Mph1103I	ATGCAT	4 cut(s)	56, 515, 1150, 1252
MroNI	GCCGGC	1 cut(s)	1603
MscI	TGGCCA	1 cut(s)	448
MseI	TTAA	3 cut(s)	180, 2124, 2214
MslI	CAYNNNNRTG	1 cut(s)	2132
Msp20I	TGGCCA	1 cut(s)	448
MspA1I	CMGCKG	2 cut(s)	1379, 1661
MspCI	CTTAAG	1 cut(s)	2213
MspI	CCGG	4 cut(s)	281, 378, 699, 1604
MspR9I	CCNGG	8 cut(s)	282, 700, 759, 1165, 1542, 1845, 2118, 2186
MunI	CAATTG	2 cut(s)	2007, 2274
Mva1269I	GAATGC	2 cut(s)	679, 1515
MvaI	CCWGG	6 cut(s)	759, 1165, 1542, 1845, 2118, 2186
MvnI	CGCG	2 cut(s)	905, 1709
NaeI	GCCGGC	1 cut(s)	1605
NciI	CCSGG	2 cut(s)	282, 700
NcoI	CCATGG	1 cut(s)	1638
NdeI	CATATG	1 cut(s)	409
NgoMIV	GCCGGC	1 cut(s)	1603
NlaIV	GGNNCC	3 cut(s)	483, 775, 1701
NmuCI	GTSAC	4 cut(s)	626, 1172, 2059, 2134
NsiI	ATGCAT	4 cut(s)	56, 515, 1150, 1252
NspI	RCATGY	1 cut(s)	513
PaqCI	CACCTGC	1 cut(s)	1468
PciSI	GCTCTTC	1 cut(s)	1268
PcsI	WCGNNNNNNNCGW	1 cut(s)	861
PctI	GAATGC	2 cut(s)	679, 1515
PdiI	GCCGGC	1 cut(s)	1605
PfeI	GAWTC	9 cut(s)	374, 593, 732, 918, 1117, 1181, 1967, 2013, 2022
PfoI	TCCNGGA	1 cut(s)	698
PkrI	GCNGC	9 cut(s)	139, 238, 790, 1003, 1049, 1378, 1561, 1645, 2300
PleI	GAGTC	8 cut(s)	460, 1036, 1169, 1358, 1553, 1571, 1732, 1796
PpsI	GAGTC	8 cut(s)	460, 1036, 1169, 1358, 1553, 1571, 1732, 1796
PpuMI	RGGWCCY	1 cut(s)	1493
PshAI	GACNNNNGTC	2 cut(s)	480, 1758
PsiI	TTATAA	1 cut(s)	1766
Psp124BI	GAGCTC	2 cut(s)	157, 847
Psp1406I	AACGTT	2 cut(s)	1089, 1230
Psp5II	RGGWCCY	1 cut(s)	1493
Psp6I	CCWGG	6 cut(s)	757, 1163, 1540, 1843, 2116, 2184
PspFI	CCCAGC	1 cut(s)	80
PspGI	CCWGG	6 cut(s)	757, 1163, 1540, 1843, 2116, 2184
PspN4I	GGNNCC	3 cut(s)	483, 775, 1701
PspPI	GGNCC	6 cut(s)	380, 482, 950, 1167, 1493, 1700
PspPPI	RGGWCCY	1 cut(s)	1493
PstI	CTGCAG	1 cut(s)	1966
PstNI	CAGNNNCTG	3 cut(s)	74, 1170, 1778
PsuI	RGATCY	3 cut(s)	659, 1383, 1411
PvuII	CAGCTG	2 cut(s)	1379, 1661
RsaI	GTAC	7 cut(s)	353, 508, 743, 964, 1041, 1288, 2258
RsaNI	GTAC	7 cut(s)	352, 507, 742, 963, 1040, 1287, 2257
RseI	CAYNNNNRTG	1 cut(s)	2132
SacI	GAGCTC	2 cut(s)	157, 847
SapI	GCTCTTC	1 cut(s)	1268
SaqAI	TTAA	3 cut(s)	180, 2124, 2214
SatI	GCNGC	9 cut(s)	138, 237, 789, 1002, 1048, 1377, 1560, 1644, 2299
Sau96I	GGNCC	6 cut(s)	380, 482, 950, 1167, 1493, 1700
SchI	GAGTC	8 cut(s)	461, 1037, 1169, 1358, 1554, 1571, 1732, 1796
ScrFI	CCNGG	8 cut(s)	282, 700, 759, 1165, 1542, 1845, 2118, 2186
SduI	GDGCHC	3 cut(s)	157, 847, 2236
SexAI	ACCWGGT	1 cut(s)	2184
SfcI	CTRYAG	1 cut(s)	1962
SinI	GGWCC	5 cut(s)	380, 482, 950, 1493, 1700
SmiMI	CAYNNNNRTG	1 cut(s)	2132
SmlI	CTYRAG	3 cut(s)	347, 1579, 2213
SmoI	CTYRAG	3 cut(s)	347, 1579, 2213
SpeI	ACTAGT	1 cut(s)	1849
SsiI	CCGC	9 cut(s)	905, 938, 960, 1047, 1257, 1536, 1707, 2087, 2299
SspMI	CTAG	7 cut(s)	729, 1043, 1187, 1260, 1317, 1329, 1850
SstI	GAGCTC	2 cut(s)	157, 847
StyD4I	CCNGG	8 cut(s)	280, 698, 757, 1163, 1540, 1843, 2116, 2184
StyI	CCWWGG	3 cut(s)	261, 983, 1638
TaiI	ACGT	4 cut(s)	1092, 1233, 1623, 1911
TaqI	TCGA	5 cut(s)	86, 372, 795, 864, 1550
TaqII	GACCGA	1 cut(s)	614
TatI	WGTACW	3 cut(s)	741, 1039, 2256
TauI	GCSGC	2 cut(s)	1050, 2301
TfiI	GAWTC	9 cut(s)	374, 593, 732, 918, 1117, 1181, 1967, 2013, 2022
Tru1I	TTAA	3 cut(s)	180, 2124, 2214
Tru9I	TTAA	3 cut(s)	180, 2124, 2214
TscAI	CASTG	5 cut(s)	775, 880, 1441, 1596, 2064
TseFI	GTSAC	4 cut(s)	626, 1172, 2059, 2134
TseI	GCWGC	7 cut(s)	137, 236, 788, 1001, 1376, 1559, 1643
Tsp45I	GTSAC	4 cut(s)	626, 1172, 2059, 2134
TspDTI	ATGAA	5 cut(s)	17, 311, 324, 378, 1109
TspGWI	ACGGA	1 cut(s)	480
TspRI	CASTG	5 cut(s)	775, 880, 1441, 1596, 2064
Vha464I	CTTAAG	1 cut(s)	2213
VpaK11BI	GGWCC	5 cut(s)	380, 482, 950, 1493, 1700
XapI	RAATTY	2 cut(s)	426, 1948
XbaI	TCTAGA	1 cut(s)	1186
XceI	RCATGY	1 cut(s)	513
XmiI	GTMKAC	3 cut(s)	105, 114, 1075
XspI	CTAG	7 cut(s)	729, 1043, 1187, 1260, 1317, 1329, 1850
Zsp2I	ATGCAT	4 cut(s)	56, 515, 1150, 1252

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.