Rroxscaffold_7G00164720

Subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008647
Physical Location & Seq
Forward (+)
6653093 .. 6655331
2239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164720.1

Sequence Viewer

Length: 1233 bp
ATGGCTCAAAAACCTGGAGTTATATCTGTTTTTCCTGATCACTTACTGAAACTCCACACCACTCATTCCTGGGATTTCCTCAAGTATCAGACTGAGTTAGAAATCGACTCATTTCCGAACAGCATCTCCGAGAATGATGATGGTGCTGCTGATGACACAGCACCGGACTCTAATGGATCTGACACCATTATTGGCATTTTAGACACAGGTATTTGGCCGGAGTCGGAGAGTTTTATTGACAAGGATATGGGTCCGATTCCATCAAGGTGGAAGGGTACATGCATGAAAGGACCTGATTTTACTTCATCCAGCTGTAATAGGAAGTTAATAGGAGCAAGATTTTACAACTCCAGCGATCTAATCACAGACAACGATTCACCAAGGGATTTAGTGGGGCATGGTACGCATGTTGCAGGCACAGCGGCAGGGAGTGTTGTGCCGGGTGCATCCTACTATGGCTTAGCTGCTGGTACAGCCAAGGGAGGCTCCCCAGGTTCAAGGATCGCCATGTATAAAGTATGCACAGCAGAGGGTTGCTATGGGTCGGCAATTTTAGCAGCATTCGATGATGCTATCAGTGATGGGGTGGATGTTCTATCTGTGTCCCTTGGTTCGTCAAGTTATCAGCCGGAGCTGAGCAGTGACCCCATTGCAATAGGAGCTTTCCATGCAGTGGAGCAGGGGATTATAGTCGTGTCCTCGGCAGGGAATGATGGACCGAACCGCGAAACAGTAGCGAACTTTGCGCCTTGGCTATTGACTGTTGCTGCCTCTACCATTGACCGGAACTTTGAGTCTAATGTGGTCTTGGGGGGAAACAAAGCGATCAAGGGAGAAGGCATAAATTTTTCTAGTCTCCAGAAAGTTCCTGTGTACCCACTGATATATGCACCATCTGCCAAGACAGCAGACGCTGAGGAACCCGAAGCAAGAAACTGCGACAGACATTCATTGGACGAAAAAATGATCAAGGGAAAGATTGTAATCTGCGACACAGATGTTCCTTTTTATACTACTGACAACCAAATTGAGGCCGTGAAGAGTCTTGGCGGAATAGGTGTAATTTTTAACAGGGATGATCATATCGGGATAATAGCAGACACATATGGAGCAGTTCCTGCAACCGCGATCAGTTTAAAGGGTGCCAAAGATATCTTCTCCTATATCAACTCAACCAGGTACACAAACAAAGCAAATCCAAATTCACCAGACCTTTCATTCAATACTGACTGA

Protein Analysis

410

Amino Acids

43.39

Weight (kDa)

4.67

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 59 - 299 2.9e-26 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1142
AccB7I CCANNNNNTGG 1 cut(s) 673
AccII CGCG 2 cut(s) 726, 1127
AciI CCGC 4 cut(s) 422, 724, 1050, 1125
AclWI GGATC 2 cut(s) 184, 509
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 2 cut(s) 844, 1201
AfaI GTAC 5 cut(s) 277, 403, 472, 875, 1181
AfiI CCNNNNNNNGG 4 cut(s) 673, 705, 783, 1030
AgsI TTSAA 2 cut(s) 498, 1222
AjnI CCWGG 4 cut(s) 13, 68, 490, 1175
AjuI GAANNNNNNNTTGG 2 cut(s) 1139, 1171
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 4 cut(s) 312, 464, 634, 662
AluI AGCT 4 cut(s) 312, 464, 634, 662
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 2 cut(s) 184, 509
AlwNI CAGNNNCTG 2 cut(s) 914, 1118
AoxI GGCC 2 cut(s) 215, 1032
ApeKI GCWGC 4 cut(s) 146, 464, 557, 767
ApoI RAATTY 2 cut(s) 844, 1201
ArsI GACNNNNNNTTYG 2 cut(s) 773, 805
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 3 cut(s) 251, 290, 716
AsuC2I CCSGG 1 cut(s) 441
AsuHPI GGTGA 2 cut(s) 369, 1197
AvaII GGWCC 3 cut(s) 251, 290, 716
BaeI ACNNNNGTAYC 2 cut(s) 393, 426
BanI GGYRCC 1 cut(s) 1142
BbvCI CCTCAGC 1 cut(s) 915
BbvI GCAGC 4 cut(s) 133, 451, 569, 754
BccI CCATC 5 cut(s) 134, 268, 575, 707, 901
BceAI ACGGC 1 cut(s) 1019
BciT130I CCWGG 4 cut(s) 15, 70, 492, 1177
BclI TGATCA 3 cut(s) 37, 966, 1078
BcnI CCSGG 1 cut(s) 441
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 1 cut(s) 852
BisI GCNGC 5 cut(s) 147, 423, 465, 558, 768
BlpI GCTNAGC 2 cut(s) 460, 635
BlsI GCNGC 5 cut(s) 148, 424, 466, 559, 769
Bme1390I CCNGG 5 cut(s) 15, 70, 441, 492, 1177
Bme18I GGWCC 3 cut(s) 251, 290, 716
BmgT120I GGNCC 3 cut(s) 251, 290, 716
BmiI GGNNCC 4 cut(s) 252, 487, 921, 1144
BmrFI CCNGG 5 cut(s) 15, 70, 441, 492, 1177
BmsI GCATC 3 cut(s) 132, 455, 559
BpmI CTGGAG 3 cut(s) 36, 334, 842
Bpu10I CCTNAGC 1 cut(s) 915
Bpu1102I GCTNAGC 2 cut(s) 460, 635
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 441
BsaBI GATNNNNATC 1 cut(s) 983
BsaJI CCNNGG 7 cut(s) 69, 380, 477, 490, 607, 699, 749
BsaWI WCCGGW 2 cut(s) 163, 783
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 110, 140
Bsc4I CCNNNNNNNGG 4 cut(s) 673, 705, 783, 1030
Bse3DI GCAATG 1 cut(s) 648
Bse8I GATNNNNATC 1 cut(s) 983
BseBI CCWGG 4 cut(s) 15, 70, 492, 1177
BseDI CCNNGG 7 cut(s) 69, 380, 477, 490, 607, 699, 749
BseGI GGATG 4 cut(s) 305, 446, 595, 1081
BseJI GATNNNNATC 1 cut(s) 983
BseLI CCNNNNNNNGG 4 cut(s) 673, 705, 783, 1030
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 3 cut(s) 84, 626, 906
BseXI GCAGC 4 cut(s) 133, 451, 569, 754
Bsh1236I CGCG 2 cut(s) 726, 1127
BshFI GGCC 2 cut(s) 217, 1034
BshNI GGYRCC 1 cut(s) 1142
BsiSI CCGG 5 cut(s) 164, 218, 440, 629, 784
BslFI GGGAC 1 cut(s) 589
BslI CCNNNNNNNGG 4 cut(s) 673, 705, 783, 1030
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 589
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 2 cut(s) 217, 1034
Bsp143I GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
Bsp1720I GCTNAGC 2 cut(s) 460, 635
BspACI CCGC 4 cut(s) 422, 724, 1050, 1125
BspANI GGCC 2 cut(s) 217, 1034
BspCNI CTCAG 3 cut(s) 85, 627, 907
BspFNI CGCG 2 cut(s) 726, 1127
BspLI GGNNCC 4 cut(s) 252, 487, 921, 1144
BspPI GGATC 2 cut(s) 184, 509
BspT107I GGYRCC 1 cut(s) 1142
BsrDI GCAATG 1 cut(s) 648
BssECI CCNNGG 7 cut(s) 69, 380, 477, 490, 607, 699, 749
BssMI GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
BssT1I CCWWGG 4 cut(s) 380, 477, 607, 749
Bst2UI CCWGG 4 cut(s) 15, 70, 492, 1177
Bst4CI ACNGT 2 cut(s) 733, 763
Bst6I CTCTTC 1 cut(s) 1034
BstAPI GCANNNNNTGC 2 cut(s) 896, 1118
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 4 cut(s) 93, 460, 635, 915
BstF5I GGATG 4 cut(s) 305, 446, 595, 1081
BstFNI CGCG 2 cut(s) 726, 1127
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 8 cut(s) 40, 179, 358, 504, 828, 969, 1081, 1131
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
BstNI CCWGG 4 cut(s) 15, 70, 492, 1177
BstNSI RCATGY 2 cut(s) 282, 410
BstSCI CCNGG 5 cut(s) 13, 68, 439, 490, 1175
BstUI CGCG 2 cut(s) 726, 1127
BstV1I GCAGC 4 cut(s) 133, 451, 569, 754
BstX2I RGATCY 1 cut(s) 176
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 2 cut(s) 217, 1034
BtsCI GGATG 4 cut(s) 305, 446, 595, 1081
BtsI GCAGTG 2 cut(s) 646, 678
BtsIMutI CAGTG 4 cut(s) 583, 646, 678, 878
Cac8I GCNNGC 1 cut(s) 415
CaiI CAGNNNCTG 2 cut(s) 914, 1118
CfoI GCGC 1 cut(s) 748
Cfr13I GGNCC 3 cut(s) 251, 290, 716
CseI GACGC 1 cut(s) 920
CsiI ACCWGGT 1 cut(s) 1175
Csp6I GTAC 5 cut(s) 276, 402, 471, 874, 1180
CviAII CATG 6 cut(s) 279, 283, 398, 407, 508, 668
CviQI GTAC 5 cut(s) 276, 402, 471, 874, 1180
DdeI CTNAG 4 cut(s) 93, 460, 635, 915
DpnI GATC 8 cut(s) 39, 178, 357, 503, 827, 968, 1080, 1130
DpnII GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
DraI TTTAAA 1 cut(s) 1137
EaeI YGGCCR 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 1034
EarI CTCTTC 1 cut(s) 1034
EciI GGCGGA 1 cut(s) 1065
Eco130I CCWWGG 4 cut(s) 380, 477, 607, 749
Eco32I GATATC 1 cut(s) 1153
Eco47I GGWCC 3 cut(s) 251, 290, 716
EcoO109I RGGNCCY 1 cut(s) 290
EcoRII CCWGG 4 cut(s) 13, 68, 490, 1175
EcoRV GATATC 1 cut(s) 1153
EcoT14I CCWWGG 4 cut(s) 380, 477, 607, 749
EcoT22I ATGCAT 1 cut(s) 284
ErhI CCWWGG 4 cut(s) 380, 477, 607, 749
FaeI CATG 6 cut(s) 282, 286, 401, 410, 511, 671
FaqI GGGAC 1 cut(s) 589
FatI CATG 6 cut(s) 278, 282, 397, 406, 507, 667
FauNDI CATATG 1 cut(s) 1105
FbaI TGATCA 3 cut(s) 37, 966, 1078
Fnu4HI GCNGC 5 cut(s) 147, 423, 465, 558, 768
FokI GGATG 4 cut(s) 292, 433, 602, 1088
Fsp4HI GCNGC 5 cut(s) 147, 423, 465, 558, 768
FspBI CTAG 1 cut(s) 852
GlaI GCGC 1 cut(s) 747
GluI GCNGC 5 cut(s) 147, 423, 465, 558, 768
GsuI CTGGAG 3 cut(s) 36, 334, 842
HaeIII GGCC 2 cut(s) 217, 1034
HapII CCGG 5 cut(s) 164, 218, 440, 629, 784
HgaI GACGC 1 cut(s) 920
HhaI GCGC 1 cut(s) 748
Hin1II CATG 6 cut(s) 282, 286, 401, 410, 511, 671
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HinfI GANTC 7 cut(s) 107, 167, 221, 256, 374, 794, 1042
HpaII CCGG 5 cut(s) 164, 218, 440, 629, 784
HphI GGTGA 2 cut(s) 369, 1197
Hpy166II GTNNAC 2 cut(s) 874, 1182
Hpy188I TCNGA 6 cut(s) 90, 117, 130, 181, 226, 255
Hpy188III TCNNGA 3 cut(s) 35, 859, 1087
Hpy8I GTNNAC 2 cut(s) 874, 1182
HpyAV CCTTC 2 cut(s) 265, 830
HpyCH4III ACNGT 2 cut(s) 733, 763
HpyCH4V TGCA 8 cut(s) 282, 413, 446, 522, 653, 671, 890, 1121
HpyF3I CTNAG 4 cut(s) 93, 460, 635, 915
Hsp92II CATG 6 cut(s) 282, 286, 401, 410, 511, 671
HspAI GCGC 1 cut(s) 746
Ksp22I TGATCA 3 cut(s) 37, 966, 1078
Kzo9I GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
LmnI GCTCC 6 cut(s) 332, 491, 631, 659, 676, 1109
Lsp1109I GCAGC 4 cut(s) 133, 451, 569, 754
LweI GCATC 3 cut(s) 132, 455, 559
MabI ACCWGGT 1 cut(s) 1175
MaeI CTAG 1 cut(s) 852
MaeIII GTNAC 1 cut(s) 641
MalI GATC 8 cut(s) 39, 178, 357, 503, 827, 968, 1080, 1130
MboI GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
MboII GAAGA 2 cut(s) 1051, 1147
MflI RGATCY 1 cut(s) 176
MluCI AATT 5 cut(s) 549, 844, 1026, 1062, 1201
MlyI GAGTC 5 cut(s) 101, 161, 230, 803, 1051
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 7 cut(s) 89, 476, 523, 709, 781, 910, 1024
Mph1103I ATGCAT 1 cut(s) 284
MseI TTAA 3 cut(s) 326, 1068, 1136
MslI CAYNNNNRTG 1 cut(s) 265
MspA1I CMGCKG 2 cut(s) 312, 422
MspI CCGG 5 cut(s) 164, 218, 440, 629, 784
MspR9I CCNGG 5 cut(s) 15, 70, 441, 492, 1177
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 4 cut(s) 15, 70, 492, 1177
MvnI CGCG 2 cut(s) 726, 1127
NciI CCSGG 1 cut(s) 441
NdeI CATATG 1 cut(s) 1105
NdeII GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
NlaIII CATG 6 cut(s) 282, 286, 401, 410, 511, 671
NlaIV GGNNCC 4 cut(s) 252, 487, 921, 1144
NmeAIII GCCGAG 1 cut(s) 680
NmuCI GTSAC 1 cut(s) 641
NsiI ATGCAT 1 cut(s) 284
NspI RCATGY 2 cut(s) 282, 410
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 2 cut(s) 256, 374
PflMI CCANNNNNTGG 1 cut(s) 673
PkrI GCNGC 5 cut(s) 148, 424, 466, 559, 769
PleI GAGTC 5 cut(s) 101, 161, 229, 802, 1050
PpsI GAGTC 5 cut(s) 101, 161, 229, 802, 1050
PpuMI RGGWCCY 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 4 cut(s) 13, 68, 490, 1175
PspGI CCWGG 4 cut(s) 13, 68, 490, 1175
PspN4I GGNNCC 4 cut(s) 252, 487, 921, 1144
PspPI GGNCC 3 cut(s) 251, 290, 716
PspPPI RGGWCCY 1 cut(s) 290
PstNI CAGNNNCTG 2 cut(s) 914, 1118
PsuI RGATCY 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 312
RsaI GTAC 5 cut(s) 277, 403, 472, 875, 1181
RsaNI GTAC 5 cut(s) 276, 402, 471, 874, 1180
RseI CAYNNNNRTG 1 cut(s) 265
SaqAI TTAA 3 cut(s) 326, 1068, 1136
SatI GCNGC 5 cut(s) 147, 423, 465, 558, 768
Sau3AI GATC 8 cut(s) 37, 176, 355, 501, 825, 966, 1078, 1128
Sau96I GGNCC 3 cut(s) 251, 290, 716
SchI GAGTC 5 cut(s) 101, 161, 230, 803, 1051
ScrFI CCNGG 5 cut(s) 15, 70, 441, 492, 1177
SexAI ACCWGGT 1 cut(s) 1175
SfaNI GCATC 3 cut(s) 132, 455, 559
SinI GGWCC 3 cut(s) 251, 290, 716
SmiMI CAYNNNNRTG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 5 cut(s) 549, 844, 1026, 1062, 1201
SsiI CCGC 4 cut(s) 422, 724, 1050, 1125
SspMI CTAG 1 cut(s) 852
StyD4I CCNGG 5 cut(s) 13, 68, 439, 490, 1175
StyI CCWWGG 4 cut(s) 380, 477, 607, 749
TaaI ACNGT 2 cut(s) 733, 763
TaqI TCGA 2 cut(s) 105, 564
TaqII GACCGA 1 cut(s) 733
TasI AATT 5 cut(s) 549, 844, 1026, 1062, 1201
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 2 cut(s) 256, 374
Tru1I TTAA 3 cut(s) 326, 1068, 1136
Tru9I TTAA 3 cut(s) 326, 1068, 1136
TscAI CASTG 4 cut(s) 583, 646, 678, 885
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 4 cut(s) 146, 464, 557, 767
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 4 cut(s) 294, 299, 939, 1206
TspRI CASTG 4 cut(s) 583, 646, 678, 885
Van91I CCANNNNNTGG 1 cut(s) 673
VpaK11BI GGWCC 3 cut(s) 251, 290, 716
XapI RAATTY 2 cut(s) 844, 1201
XceI RCATGY 2 cut(s) 282, 410
XspI CTAG 1 cut(s) 852
Zsp2I ATGCAT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.