Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008647

Physical Location & Seq

Genomic Coordinates Forward (+)

697893 .. 700075

Gene Structure 2183 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1009400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1260 bp

ATGAAAAACATCTTCGTTTTCATGTCGTCCGGTACTTTTCTCTTGCTCCTGATCATCATCTCGTTTCATGGACCAACTAAAGCAGCTGATGATCATCAGAATGCCGGGAGAGGGAGTCGGAGAGACCGCCTTTACATCGTGTATATGGGATCTGCAGCCGCCTCATCAACAACTAGTGATGATGATATTTCTTCCCTAAGGGATGATCATGCTCAGCTCCTCAGTTCTGTCGTAACAAGGAAACCAAATGCAGTGGTGCACACTTACAAGCATGGTTTTTCAGGATTTGCGGCTCGTCTCACGGACGAAGAGGCGCGATCCATTGCTTCAAAGCCTGGAGTTGTATCTGTTTTTCTAGATCCCATATTGAAACTCCACACTACTCATTCCTGGGATTTCCTCAAGTATCAAACTGCTTTAAAAATCGACTCAAATCTTAACTCTAACAACTATGATGCCGCGGACGGAGTTTCATTAGTTACCGCTGATGGATCTGACACCATTATCGGTATTTTGGACACAGGTATATGGCCGGAGTCGGAGAGTTTCAATGACCAAGATATGGGTCCTATTCCGTCACGGTGGAAGGGTACGTGCATGAAATCAGATGATTTCAGTTCCTCCAACTGTAATAGGAAGTTGATAGGAGCAAGGTTTTATAGCACTCCTGAATCTGATGGCACAATAGAGATTGATTCCCCAAGGGATACAGTGGGACACGGCAGCCATGTGGCCGGAACAGCAGCAGGGACCCCTGTATCAGATGCGTCCTACTACGGCGTAGCAGCTGGGACTGCCATAGGAGGCTCCCCGGGTTCAAGGATCGCCATGTACAAGGTATGCTCGGAAGGAGGTTGTCGTGGCTCCGACATTTTAGCTGCCTTTGACGACGCGATTGCGGACGGGGTGGACGTTCTATCCCTCTCGCTTGGTTCAGCCAGTGGGTTTCAGCCGGAGTTGAGCAACGACCCCATTGCGATCGGAGCGTTCCATGCGGTGGAGCAGGGGATTACCGTGGTCTGCTCCGCCGGGAATGATGGCCCTGACCCTGGAACCGTTGTGAACGCCGCACCCTGGATTTTGACAGTTGGCGCCACCACCATTGACCGAGATTTCGAGTCTGATGTGGTGTTGGGAGGCGGCACGGTGGTCAAGGGGAGAGCCATAAATTTTTCAAAACTCGAAAAATCGCCTGTGTACCCGTTGATATATGCTCTGTCTGCCCGCAAAGCTGATGGTAATGAACATAGTGCAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0010033 GO:0010037 GO:0016787 GO:0017171 GO:0019538 GO:0030312 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0042221 GO:0042493 GO:0043170 GO:0043900 GO:0043901 GO:0044238 GO:0044464 GO:0048046 GO:0048519 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0065007 GO:0070011 GO:0071704 GO:0071944 GO:0072347 GO:0080134 GO:0140096 GO:1900424 GO:1900425 GO:1901564 GO:1901700 GO:2000026 GO:2000038 GO:2000122

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

420

Amino Acids

44.11

Weight (kDa)

5.1

Isoelectric Point (pI)

29.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	45 - 126	4.7e-19	Peptidase inhibitor I9
Peptidase_S8	PF00082	164 - 383	2.5e-25	Subtilase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008647)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20150 AT1G20160 AT1G20160 AT1G20160
fragaria_vesca	FvH4_2g38090
malus_domestica	MD08G1009400.v1.1
prunus_persica	Prupe.1G361300_v2.0.a1
pyrus_communis	pycom08g00790 pycom15g00680 pycom15g00690
rosa_chinensis	RchiOBHm_Chr3g0451701 RchiOBHm_Chr6g0303461
rosa_laevigata	RLG00000011082
rosa_multiflora	Rmu_ssc0000114.1_g000050
rosa_roxburghii	Rroxscaffold_7G00164720 Rroxscaffold_7G00164730
rosa_rugosa	Rorug06G0321400 Rorug06G0321500
rosa_samantha	Rh6AG436000 Rh6BG467600 Rh6CG447900 Rh6DG434600
rosa_wichuraiana	Rw6G037730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1091
AccB7I	CCANNNNNTGG	2 cut(s)	562, 997
AccII	CGCG	3 cut(s)	316, 461, 893
AclWI	GGATC	5 cut(s)	157, 312, 353, 499, 830
AcoI	YGGCCR	2 cut(s)	530, 732
AcsI	RAATTY	1 cut(s)	1168
AcyI	GRCGYC	1 cut(s)	1092
AfaI	GTAC	4 cut(s)	34, 592, 833, 1199
AfiI	CCNNNNNNNGG	5 cut(s)	111, 562, 997, 1049, 1074
AgsI	TTSAA	5 cut(s)	330, 370, 550, 819, 1176
AhlI	ACTAGT	1 cut(s)	173
AjnI	CCWGG	4 cut(s)	334, 389, 1048, 1073
AluBI	AGCT	5 cut(s)	86, 217, 788, 878, 1232
AluI	AGCT	5 cut(s)	86, 217, 788, 878, 1232
Alw21I	GWGCWC	1 cut(s)	261
Alw26I	GTCTC	2 cut(s)	117, 302
Alw44I	GTGCAC	1 cut(s)	257
AlwI	GGATC	5 cut(s)	157, 312, 353, 499, 830
Ama87I	CYCGRG	1 cut(s)	811
AoxI	GGCC	3 cut(s)	530, 732, 1039
ApaLI	GTGCAC	1 cut(s)	257
ApeKI	GCWGC	6 cut(s)	83, 155, 723, 743, 785, 878
ApoI	RAATTY	1 cut(s)	1168
ArsI	GACNNNNNNTTYG	2 cut(s)	1097, 1129
AspLEI	GCGC	2 cut(s)	316, 1094
AspS9I	GGNCC	4 cut(s)	71, 566, 750, 1040
AsuC2I	CCSGG	4 cut(s)	106, 812, 813, 1030
AvaI	CYCGRG	1 cut(s)	811
AvaII	GGWCC	3 cut(s)	71, 566, 750
AxyI	CCTNAGG	1 cut(s)	197
BaeGI	GKGCMC	1 cut(s)	261
BaeI	ACNNNNGTAYC	2 cut(s)	741, 774
BanI	GGYRCC	1 cut(s)	1091
Bbv12I	GWGCWC	1 cut(s)	261
BbvI	GCAGC	6 cut(s)	95, 167, 735, 755, 797, 865
BccI	CCATC	4 cut(s)	482, 671, 1031, 1229
BceAI	ACGGC	2 cut(s)	736, 793
BcgI	CGANNNNNNTGC	4 cut(s)	878, 912, 956, 990
BciT130I	CCWGG	4 cut(s)	336, 391, 1050, 1075
BciVI	GTATCC	1 cut(s)	700
BclI	TGATCA	3 cut(s)	51, 91, 205
BcnI	CCSGG	4 cut(s)	106, 812, 813, 1030
BcoDI	GTCTC	2 cut(s)	117, 302
BcuI	ACTAGT	1 cut(s)	173
BfaI	CTAG	2 cut(s)	174, 356
BfmI	CTRYAG	1 cut(s)	153
BfoI	RGCGCY	1 cut(s)	1095
BfuI	GTATCC	1 cut(s)	700
BlpI	GCTNAGC	1 cut(s)	213
Bme1390I	CCNGG	8 cut(s)	106, 336, 391, 812, 813, 1030, 1050, 1075
Bme18I	GGWCC	3 cut(s)	71, 566, 750
BmeT110I	CYCGRG	1 cut(s)	811
BmgT120I	GGNCC	4 cut(s)	71, 566, 750, 1040
BmiI	GGNNCC	7 cut(s)	567, 751, 752, 808, 865, 1054, 1093
BmrFI	CCNGG	8 cut(s)	106, 336, 391, 812, 813, 1030, 1050, 1075
BmsI	GCATC	2 cut(s)	445, 754
BpmI	CTGGAG	1 cut(s)	357
Bpu1102I	GCTNAGC	1 cut(s)	213
BpuEI	CTTGAG	1 cut(s)	386
BpuMI	CCSGG	4 cut(s)	106, 812, 813, 1030
BsaAI	YACGTR	1 cut(s)	594
BsaBI	GATNNNNATC	2 cut(s)	56, 93
BsaHI	GRCGYC	1 cut(s)	1092
BsaI	GGTCTC	1 cut(s)	117
BsaJI	CCNNGG	8 cut(s)	390, 459, 701, 810, 811, 1014, 1048, 1073
BsaWI	WCCGGW	1 cut(s)	29
BsaXI	ACNNNNNCTCC	2 cut(s)	100, 130
Bsc4I	CCNNNNNNNGG	5 cut(s)	111, 562, 997, 1049, 1074
Bse1I	ACTGG	1 cut(s)	939
Bse21I	CCTNAGG	1 cut(s)	197
Bse3DI	GCAATG	2 cut(s)	321, 972
Bse8I	GATNNNNATC	2 cut(s)	56, 93
BseBI	CCWGG	4 cut(s)	336, 391, 1050, 1075
BseDI	CCNNGG	8 cut(s)	390, 459, 701, 810, 811, 1014, 1048, 1073
BseGI	GGATG	1 cut(s)	208
BseJI	GATNNNNATC	2 cut(s)	56, 93
BseLI	CCNNNNNNNGG	5 cut(s)	111, 562, 997, 1049, 1074
BseMI	GCAATG	2 cut(s)	321, 972
BseMII	CTCAG	2 cut(s)	227, 235
BseNI	ACTGG	1 cut(s)	939
BseRI	GAGGAG	1 cut(s)	209
BseSI	GKGCMC	1 cut(s)	261
BseXI	GCAGC	6 cut(s)	95, 167, 735, 755, 797, 865
BseYI	CCCAGC	1 cut(s)	788
Bsh1236I	CGCG	3 cut(s)	316, 461, 893
Bsh1285I	CGRYCG	1 cut(s)	981
BshFI	GGCC	3 cut(s)	532, 734, 1041
BshNI	GGYRCC	1 cut(s)	1091
BsiEI	CGRYCG	1 cut(s)	981
BsiHKAI	GWGCWC	1 cut(s)	261
BsiHKCI	CYCGRG	1 cut(s)	811
BsiSI	CCGG	7 cut(s)	30, 105, 533, 735, 812, 953, 1029
BslFI	GGGAC	3 cut(s)	729, 763, 805
BslI	CCNNNNNNNGG	5 cut(s)	111, 562, 997, 1049, 1074
BsmAI	GTCTC	2 cut(s)	117, 302
BsmBI	CGTCTC	1 cut(s)	302
BsmFI	GGGAC	3 cut(s)	729, 763, 805
BsmI	GAATGC	1 cut(s)	106
BsnI	GGCC	3 cut(s)	532, 734, 1041
Bso31I	GGTCTC	1 cut(s)	117
BsoBI	CYCGRG	1 cut(s)	811
Bsp1286I	GDGCHC	1 cut(s)	261
Bsp1407I	TGTACA	1 cut(s)	831
Bsp143I	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
Bsp1720I	GCTNAGC	1 cut(s)	213
BspANI	GGCC	3 cut(s)	532, 734, 1041
BspCNI	CTCAG	2 cut(s)	226, 234
BspFNI	CGCG	3 cut(s)	316, 461, 893
BspLI	GGNNCC	7 cut(s)	567, 751, 752, 808, 865, 1054, 1093
BspMAI	CTGCAG	1 cut(s)	157
BspPI	GGATC	5 cut(s)	157, 312, 353, 499, 830
BspT107I	GGYRCC	1 cut(s)	1091
BspTNI	GGTCTC	1 cut(s)	117
BsrDI	GCAATG	2 cut(s)	321, 972
BsrGI	TGTACA	1 cut(s)	831
BsrI	ACTGG	1 cut(s)	939
BssECI	CCNNGG	8 cut(s)	390, 459, 701, 810, 811, 1014, 1048, 1073
BssMI	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
BssNI	GRCGYC	1 cut(s)	1092
BssT1I	CCWWGG	1 cut(s)	701
Bst2UI	CCWGG	4 cut(s)	336, 391, 1050, 1075
Bst4CI	ACNGT	7 cut(s)	582, 629, 712, 1015, 1057, 1087, 1147
Bst6I	CTCTTC	1 cut(s)	303
BstACI	GRCGYC	1 cut(s)	1092
BstAUI	TGTACA	1 cut(s)	831
BstBAI	YACGTR	1 cut(s)	594
BstC8I	GCNNGC	1 cut(s)	1225
BstDEI	CTNAG	3 cut(s)	197, 213, 221
BstDSI	CCRYGG	2 cut(s)	459, 1014
BstF5I	GGATG	1 cut(s)	208
BstFNI	CGCG	3 cut(s)	316, 461, 893
BstH2I	RGCGCY	1 cut(s)	1095
BstHHI	GCGC	2 cut(s)	316, 1094
BstKTI	GATC	9 cut(s)	54, 94, 152, 208, 320, 361, 494, 825, 981
BstMAI	GTCTC	2 cut(s)	117, 302
BstMBI	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
BstMCI	CGRYCG	1 cut(s)	981
BstMWI	GCNNNNNNNGC	6 cut(s)	740, 794, 983, 992, 1220, 1229
BstNI	CCWGG	4 cut(s)	336, 391, 1050, 1075
BstSCI	CCNGG	8 cut(s)	104, 334, 389, 810, 811, 1028, 1048, 1073
BstSFI	CTRYAG	1 cut(s)	153
BstSLI	GKGCMC	1 cut(s)	261
BstUI	CGCG	3 cut(s)	316, 461, 893
BstV1I	GCAGC	6 cut(s)	95, 167, 735, 755, 797, 865
BstX2I	RGATCY	3 cut(s)	149, 358, 491
BstYI	RGATCY	3 cut(s)	149, 358, 491
Bsu36I	CCTNAGG	1 cut(s)	197
BsuI	GTATCC	1 cut(s)	700
BsuRI	GGCC	3 cut(s)	532, 734, 1041
BtgI	CCRYGG	2 cut(s)	459, 1014
BtsCI	GGATG	1 cut(s)	208
BtsI	GCAGTG	1 cut(s)	258
BtsIMutI	CAGTG	3 cut(s)	258, 717, 946
Cac8I	GCNNGC	1 cut(s)	1225
CfoI	GCGC	2 cut(s)	316, 1094
Cfr13I	GGNCC	4 cut(s)	71, 566, 750, 1040
Cfr42I	CCGCGG	1 cut(s)	462
Cfr9I	CCCGGG	1 cut(s)	811
CseI	GACGC	2 cut(s)	756, 899
Csp6I	GTAC	4 cut(s)	33, 591, 832, 1198
CspCI	CAANNNNNGTGG	4 cut(s)	234, 269, 1084, 1119
CviAII	CATG	8 cut(s)	22, 68, 209, 272, 598, 728, 829, 992
CviQI	GTAC	4 cut(s)	33, 591, 832, 1198
DdeI	CTNAG	3 cut(s)	197, 213, 221
DinI	GGCGCC	1 cut(s)	1093
DpnI	GATC	9 cut(s)	53, 93, 151, 207, 319, 360, 493, 824, 980
DpnII	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
DraI	TTTAAA	1 cut(s)	420
EaeI	YGGCCR	2 cut(s)	530, 732
Eam1104I	CTCTTC	1 cut(s)	303
EarI	CTCTTC	1 cut(s)	303
EciI	GGCGGA	1 cut(s)	1015
Eco130I	CCWWGG	1 cut(s)	701
Eco31I	GGTCTC	1 cut(s)	117
Eco47I	GGWCC	3 cut(s)	71, 566, 750
Eco81I	CCTNAGG	1 cut(s)	197
Eco88I	CYCGRG	1 cut(s)	811
EcoO109I	RGGNCCY	2 cut(s)	566, 750
EcoRII	CCWGG	4 cut(s)	334, 389, 1048, 1073
EcoT14I	CCWWGG	1 cut(s)	701
EgeI	GGCGCC	1 cut(s)	1093
EheI	GGCGCC	1 cut(s)	1093
ErhI	CCWWGG	1 cut(s)	701
Esp3I	CGTCTC	1 cut(s)	302
FaeI	CATG	8 cut(s)	25, 71, 212, 275, 601, 731, 832, 995
FaqI	GGGAC	3 cut(s)	729, 763, 805
FatI	CATG	8 cut(s)	21, 67, 208, 271, 597, 727, 828, 991
FauI	CCCGC	1 cut(s)	1232
FbaI	TGATCA	3 cut(s)	51, 91, 205
FokI	GGATG	1 cut(s)	215
FspBI	CTAG	2 cut(s)	174, 356
GlaI	GCGC	2 cut(s)	315, 1093
GsaI	CCCAGC	1 cut(s)	792
GsuI	CTGGAG	1 cut(s)	357
HaeII	RGCGCY	1 cut(s)	1095
HaeIII	GGCC	3 cut(s)	532, 734, 1041
HapII	CCGG	7 cut(s)	30, 105, 533, 735, 812, 953, 1029
HgaI	GACGC	2 cut(s)	756, 899
HhaI	GCGC	2 cut(s)	316, 1094
Hin1I	GRCGYC	1 cut(s)	1092
Hin1II	CATG	8 cut(s)	25, 71, 212, 275, 601, 731, 832, 995
Hin6I	GCGC	2 cut(s)	314, 1092
HinP1I	GCGC	2 cut(s)	314, 1092
HinfI	GANTC	6 cut(s)	115, 428, 536, 671, 695, 1118
HpaII	CCGG	7 cut(s)	30, 105, 533, 735, 812, 953, 1029
Hpy166II	GTNNAC	4 cut(s)	259, 910, 1063, 1198
Hpy188III	TCNNGA	4 cut(s)	49, 282, 356, 668
Hpy8I	GTNNAC	4 cut(s)	259, 910, 1063, 1198
Hpy99I	CGWCG	1 cut(s)	893
HpyAV	CCTTC	2 cut(s)	580, 842
HpyCH4III	ACNGT	7 cut(s)	582, 629, 712, 1015, 1057, 1087, 1147
HpyCH4IV	ACGT	2 cut(s)	593, 912
HpyCH4V	TGCA	5 cut(s)	155, 251, 259, 597, 1253
HpyF10VI	GCNNNNNNNGC	6 cut(s)	740, 794, 983, 992, 1220, 1229
HpyF3I	CTNAG	3 cut(s)	197, 213, 221
HpySE526I	ACGT	2 cut(s)	593, 912
Hsp92I	GRCGYC	1 cut(s)	1092
Hsp92II	CATG	8 cut(s)	25, 71, 212, 275, 601, 731, 832, 995
HspAI	GCGC	2 cut(s)	314, 1092
KasI	GGCGCC	1 cut(s)	1091
KflI	GGGWCCC	1 cut(s)	750
Ksp22I	TGATCA	3 cut(s)	51, 91, 205
KspI	CCGCGG	1 cut(s)	462
Kzo9I	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
LmnI	GCTCC	8 cut(s)	51, 222, 647, 812, 869, 983, 1000, 1028
Lsp1109I	GCAGC	6 cut(s)	95, 167, 735, 755, 797, 865
LweI	GCATC	2 cut(s)	445, 754
MaeI	CTAG	2 cut(s)	174, 356
MaeII	ACGT	2 cut(s)	593, 912
MaeIII	GTNAC	3 cut(s)	232, 478, 576
MalI	GATC	9 cut(s)	53, 93, 151, 207, 319, 360, 493, 824, 980
MboI	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
MboII	GAAGA	3 cut(s)	4, 183, 320
MflI	RGATCY	3 cut(s)	149, 358, 491
MhlI	GDGCHC	1 cut(s)	261
MluCI	AATT	1 cut(s)	1168
Mly113I	GGCGCC	1 cut(s)	1092
MlyI	GAGTC	4 cut(s)	124, 422, 545, 1127
MmeI	TCCRAC	4 cut(s)	98, 519, 648, 891
MseI	TTAA	2 cut(s)	419, 438
MslI	CAYNNNNRTG	1 cut(s)	99
MspA1I	CMGCKG	4 cut(s)	86, 461, 485, 788
MspI	CCGG	7 cut(s)	30, 105, 533, 735, 812, 953, 1029
MspR9I	CCNGG	8 cut(s)	106, 336, 391, 812, 813, 1030, 1050, 1075
Mva1269I	GAATGC	1 cut(s)	106
MvaI	CCWGG	4 cut(s)	336, 391, 1050, 1075
MvnI	CGCG	3 cut(s)	316, 461, 893
MwoI	GCNNNNNNNGC	6 cut(s)	740, 794, 983, 992, 1220, 1229
NarI	GGCGCC	1 cut(s)	1092
NciI	CCSGG	4 cut(s)	106, 812, 813, 1030
NdeII	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
NlaIII	CATG	8 cut(s)	25, 71, 212, 275, 601, 731, 832, 995
NlaIV	GGNNCC	7 cut(s)	567, 751, 752, 808, 865, 1054, 1093
NmuCI	GTSAC	1 cut(s)	576
PcsI	WCGNNNNNNNCGW	1 cut(s)	909
PctI	GAATGC	1 cut(s)	106
PfeI	GAWTC	2 cut(s)	671, 695
PflMI	CCANNNNNTGG	2 cut(s)	562, 997
Ple19I	CGATCG	1 cut(s)	981
PleI	GAGTC	4 cut(s)	123, 422, 544, 1126
PluTI	GGCGCC	1 cut(s)	1095
PpsI	GAGTC	4 cut(s)	123, 422, 544, 1126
Ppu21I	YACGTR	1 cut(s)	594
PpuMI	RGGWCCY	2 cut(s)	566, 750
Psp5II	RGGWCCY	2 cut(s)	566, 750
Psp6I	CCWGG	4 cut(s)	334, 389, 1048, 1073
PspFI	CCCAGC	1 cut(s)	788
PspGI	CCWGG	4 cut(s)	334, 389, 1048, 1073
PspN4I	GGNNCC	7 cut(s)	567, 751, 752, 808, 865, 1054, 1093
PspPI	GGNCC	4 cut(s)	71, 566, 750, 1040
PspPPI	RGGWCCY	2 cut(s)	566, 750
PstI	CTGCAG	1 cut(s)	157
PsuI	RGATCY	3 cut(s)	149, 358, 491
PvuI	CGATCG	1 cut(s)	981
PvuII	CAGCTG	2 cut(s)	86, 788
RsaI	GTAC	4 cut(s)	34, 592, 833, 1199
RsaNI	GTAC	4 cut(s)	33, 591, 832, 1198
RseI	CAYNNNNRTG	1 cut(s)	99
SacII	CCGCGG	1 cut(s)	462
SaqAI	TTAA	2 cut(s)	419, 438
Sau3AI	GATC	9 cut(s)	51, 91, 149, 205, 317, 358, 491, 822, 978
Sau96I	GGNCC	4 cut(s)	71, 566, 750, 1040
SchI	GAGTC	4 cut(s)	124, 422, 545, 1127
ScrFI	CCNGG	8 cut(s)	106, 336, 391, 812, 813, 1030, 1050, 1075
SduI	GDGCHC	1 cut(s)	261
SfaNI	GCATC	2 cut(s)	445, 754
SfcI	CTRYAG	1 cut(s)	153
SfoI	GGCGCC	1 cut(s)	1093
Sfr303I	CCGCGG	1 cut(s)	462
SgrBI	CCGCGG	1 cut(s)	462
SinI	GGWCC	3 cut(s)	71, 566, 750
SmaI	CCCGGG	1 cut(s)	813
SmiMI	CAYNNNNRTG	1 cut(s)	99
SmlI	CTYRAG	1 cut(s)	401
SmoI	CTYRAG	1 cut(s)	401
SpeI	ACTAGT	1 cut(s)	173
Sse9I	AATT	1 cut(s)	1168
SspDI	GGCGCC	1 cut(s)	1091
SspMI	CTAG	2 cut(s)	174, 356
StyD4I	CCNGG	8 cut(s)	104, 334, 389, 810, 811, 1028, 1048, 1073
StyI	CCWWGG	1 cut(s)	701
TaaI	ACNGT	7 cut(s)	582, 629, 712, 1015, 1057, 1087, 1147
TaiI	ACGT	2 cut(s)	596, 915
TaqI	TCGA	3 cut(s)	426, 1116, 1182
TaqII	GACCGA	1 cut(s)	1122
TasI	AATT	1 cut(s)	1168
TatI	WGTACW	1 cut(s)	831
TauI	GCSGC	5 cut(s)	161, 293, 461, 1070, 1143
TfiI	GAWTC	2 cut(s)	671, 695
Tru1I	TTAA	2 cut(s)	419, 438
Tru9I	TTAA	2 cut(s)	419, 438
TscAI	CASTG	3 cut(s)	258, 717, 946
TseFI	GTSAC	1 cut(s)	576
TseI	GCWGC	6 cut(s)	83, 155, 723, 743, 785, 878
Tsp45I	GTSAC	1 cut(s)	576
TspDTI	ATGAA	6 cut(s)	10, 17, 56, 462, 614, 1257
TspGWI	ACGGA	3 cut(s)	317, 480, 564
TspMI	CCCGGG	1 cut(s)	811
TspRI	CASTG	3 cut(s)	258, 717, 946
Van91I	CCANNNNNTGG	2 cut(s)	562, 997
VneI	GTGCAC	1 cut(s)	257
VpaK11BI	GGWCC	3 cut(s)	71, 566, 750
XapI	RAATTY	1 cut(s)	1168
XbaI	TCTAGA	1 cut(s)	355
XmaI	CCCGGG	1 cut(s)	811
XspI	CTAG	2 cut(s)	174, 356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.