AT1G20180

Protein of unknown function (DUF677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011075
Physical Location & Seq
Forward (+)
6996176 .. 6997943
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20180.1

Sequence Viewer

Length: 1173 bp
ATGTTGAAAGTGAAGAATTTTCTTGGTTCTAGATTAAAGTCTTTCCCTACAAGAAAAGGAAAGTCCGACAAGGAATGTTGTCGTAGCTTGACGTCGAAGCTAAGTGTTAATGAAGAGTACAAGGAAGCATTTAGAACAAACTCCTACTTAGAGACTCGGACAAAAGCAGAAGATCAATTAGGCATAACATCATGTAGCAAACTCTCTTCTTCTTCTCCTTCTCCTTCTTCATCTTCAGATCTTAGCTTCCATTCACACTTCACCGATTACTTGCTTGACCCTCCACAAGAAACACTTGATGCCCTGATGCAAGACTCGAGTTTAGACAATCTTATAGTTACGTTTTTCGACTTGAGCTCCGAAGCTTGTGACGTGTGTGAGACTCTTCTTCAGTGCCTCCAACAAATCAAGATTAATCACAACAAAATCAAAAGGGTTATGAAGATTGGGAAAAGGGTTTGTAACGGTGCTAAAACCTTGGAATGTAGCCCCGAGATGCTCTGTGCTTTGATATTTCAAGAACTTTCGAGATTTGCCGCGCTGAAAAACCCTTTGTGTCGTATCGTCAATGAAGCACAGTTTCGTATAGTTCACGATGCAAACTCGGATTTGCTCACCAAACTGACATCGAAAAAGAGAAGAATAAGAAGAAAAATCAGGTTCTTTAAATTCTGCAAGAAGCTAGGAGGTTATAGCCTAGTGATTACTCATAGCGCGATTGTCATTACATTGCTGATCATAGCTCTTCACAGCATACTCGGCGTTTTCGCAGCTCCTGCGTTGCTCGGTCTATGTTCGTTCTGTCTTTTGAGGAAGAAGAAAGCGAAAGGAAGAATGCATAAGAGCAATAAAGATACAACTCTAGAGAAGCTCGGGACACAAATCGACATTGCAGCTAAAGGAATGTTCATATTGATAAACGATTTGGATACGTTGAGTAGACTTGCTGGGAGATTATGCGATGAGATAGAGCATAGAAAAACAGTAGCTGCGATGTGCGCAAAGAGTAGGAAGATTGAAGTGTTAAAGGAAGCATTGAGGGAGTTTAATGGACATGAAGAAAAATTCTCAGATCAATTGCAAGAGCTTGAAGAACATCTCTATCTATGTTTCCACACTATTAACCGATCAAGAAGGCTAGTGTTAGCGCAAATCACAGGACAAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

390

Amino Acids

44.07

Weight (kDa)

9.36

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 100 - 385 7.5e-17 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 95
Acc16I TGCGCA 1 cut(s) 1000
AccI GTMKAC 1 cut(s) 940
AccII CGCG 2 cut(s) 539, 716
AciI CCGC 1 cut(s) 537
AcsI RAATTY 3 cut(s) 16, 668, 1064
AcuI CTGAAG 2 cut(s) 219, 374
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 4 cut(s) 7, 518, 1019, 1091
AjiI CACGTC 1 cut(s) 373
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 2 cut(s) 146, 374
AlwNI CAGNNNCTG 2 cut(s) 776, 989
Ama87I CYCGRG 3 cut(s) 316, 491, 872
ApeKI GCWGC 3 cut(s) 770, 893, 989
ApoI RAATTY 3 cut(s) 16, 668, 1064
AseI ATTAAT 1 cut(s) 414
AspLEI GCGC 4 cut(s) 541, 716, 1001, 1150
AsuHPI GGTGA 2 cut(s) 253, 607
AvaI CYCGRG 3 cut(s) 316, 491, 872
BanII GRGCYC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 3 cut(s) 782, 905, 976
BciVI GTATCC 1 cut(s) 922
BclI TGATCA 1 cut(s) 735
BcoDI GTCTC 2 cut(s) 146, 374
BfaI CTAG 5 cut(s) 30, 683, 698, 863, 1139
BfuI GTATCC 1 cut(s) 922
BglII AGATCT 1 cut(s) 238
BisI GCNGC 4 cut(s) 537, 771, 894, 990
BlsI GCNGC 4 cut(s) 538, 772, 895, 991
BmeT110I CYCGRG 3 cut(s) 316, 491, 872
BmgBI CACGTC 1 cut(s) 373
BmsI GCATC 4 cut(s) 289, 297, 486, 586
BpuEI CTTGAG 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 477
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bse3DI GCAATG 2 cut(s) 728, 888
BseDI CCNNGG 1 cut(s) 477
BseMI GCAATG 2 cut(s) 728, 888
BseMII CTCAG 1 cut(s) 1083
BseXI GCAGC 3 cut(s) 782, 905, 976
BseYI CCCAGC 1 cut(s) 947
Bsh1236I CGCG 2 cut(s) 539, 716
BsiHKAI GWGCWC 1 cut(s) 359
BsiHKCI CYCGRG 3 cut(s) 316, 491, 872
BslFI GGGAC 1 cut(s) 889
BsmAI GTCTC 2 cut(s) 146, 374
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 840
BsoBI CYCGRG 3 cut(s) 316, 491, 872
Bsp1286I GDGCHC 1 cut(s) 359
Bsp143I GATC 5 cut(s) 172, 238, 735, 1072, 1127
BspACI CCGC 1 cut(s) 537
BspCNI CTCAG 1 cut(s) 1082
BspFNI CGCG 2 cut(s) 539, 716
BspQI GCTCTTC 1 cut(s) 750
BsrDI GCAATG 2 cut(s) 728, 888
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 5 cut(s) 172, 238, 735, 1072, 1127
BssNI GRCGYC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 3 cut(s) 467, 579, 985
Bst6I CTCTTC 4 cut(s) 108, 211, 390, 750
BstACI GRCGYC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 776
BstDEI CTNAG 4 cut(s) 101, 148, 242, 1069
BstFNI CGCG 2 cut(s) 539, 716
BstHHI GCGC 4 cut(s) 541, 716, 1001, 1150
BstKTI GATC 5 cut(s) 175, 241, 738, 1075, 1130
BstMAI GTCTC 2 cut(s) 146, 374
BstMBI GATC 5 cut(s) 172, 238, 735, 1072, 1127
BstMWI GCNNNNNNNGC 3 cut(s) 759, 776, 998
BstUI CGCG 2 cut(s) 539, 716
BstV1I GCAGC 3 cut(s) 782, 905, 976
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuI GTATCC 1 cut(s) 922
BtgZI GCGATG 2 cut(s) 975, 1007
BtrI CACGTC 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 398
CaiI CAGNNNCTG 2 cut(s) 776, 989
CfoI GCGC 4 cut(s) 541, 716, 1001, 1150
Csp6I GTAC 1 cut(s) 118
CviAII CATG 2 cut(s) 192, 1055
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 4 cut(s) 101, 148, 242, 1069
DpnI GATC 5 cut(s) 174, 240, 737, 1074, 1129
DpnII GATC 5 cut(s) 172, 238, 735, 1072, 1127
DraI TTTAAA 1 cut(s) 667
Eam1104I CTCTTC 4 cut(s) 108, 211, 390, 750
EarI CTCTTC 4 cut(s) 108, 211, 390, 750
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 359
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 219, 374
Eco88I CYCGRG 3 cut(s) 316, 491, 872
EcoICRI GAGCTC 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 840
EcoT38I GRGCYC 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 2 cut(s) 195, 1058
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 889
FatI CATG 2 cut(s) 191, 1054
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 1 cut(s) 940
Fnu4HI GCNGC 4 cut(s) 537, 771, 894, 990
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 4 cut(s) 537, 771, 894, 990
FspBI CTAG 5 cut(s) 30, 683, 698, 863, 1139
FspI TGCGCA 1 cut(s) 1000
GlaI GCGC 4 cut(s) 540, 715, 1000, 1149
GluI GCNGC 4 cut(s) 537, 771, 894, 990
GsaI CCCAGC 1 cut(s) 951
HhaI GCGC 4 cut(s) 541, 716, 1001, 1150
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 2 cut(s) 195, 1058
Hin6I GCGC 4 cut(s) 539, 714, 999, 1148
HinP1I GCGC 4 cut(s) 539, 714, 999, 1148
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 3 cut(s) 154, 314, 382
HphI GGTGA 2 cut(s) 253, 607
Hpy166II GTNNAC 2 cut(s) 592, 941
Hpy188I TCNGA 6 cut(s) 67, 159, 238, 361, 607, 1072
Hpy188III TCNNGA 9 cut(s) 30, 409, 518, 528, 593, 863, 874, 1131, 1170
Hpy8I GTNNAC 2 cut(s) 592, 941
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 228, 234, 1128
HpyCH4III ACNGT 3 cut(s) 467, 579, 985
HpyCH4IV ACGT 4 cut(s) 92, 341, 372, 932
HpyCH4V TGCA 6 cut(s) 310, 599, 675, 838, 893, 1081
HpyF10VI GCNNNNNNNGC 3 cut(s) 759, 776, 998
HpyF3I CTNAG 4 cut(s) 101, 148, 242, 1069
HpySE526I ACGT 4 cut(s) 92, 341, 372, 932
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 2 cut(s) 195, 1058
HspAI GCGC 4 cut(s) 539, 714, 999, 1148
Ksp22I TGATCA 1 cut(s) 735
Kzo9I GATC 5 cut(s) 172, 238, 735, 1072, 1127
LguI GCTCTTC 1 cut(s) 750
LmnI GCTCC 2 cut(s) 362, 778
LpnPI CCDG 5 cut(s) 317, 643, 789, 933, 1143
Lsp1109I GCAGC 3 cut(s) 782, 905, 976
LweI GCATC 4 cut(s) 289, 297, 486, 586
MaeI CTAG 5 cut(s) 30, 683, 698, 863, 1139
MaeII ACGT 4 cut(s) 92, 341, 372, 932
MaeIII GTNAC 3 cut(s) 337, 368, 461
MalI GATC 5 cut(s) 174, 240, 737, 1074, 1129
MboI GATC 5 cut(s) 172, 238, 735, 1072, 1127
MfeI CAATTG 1 cut(s) 1076
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 359
MluCI AATT 5 cut(s) 16, 176, 668, 1064, 1076
MlyI GAGTC 3 cut(s) 148, 308, 376
MmeI TCCRAC 2 cut(s) 90, 424
MnlI CCTC 5 cut(s) 291, 407, 680, 804, 1032
Mph1103I ATGCAT 1 cut(s) 840
MseI TTAA 7 cut(s) 35, 108, 414, 666, 1025, 1047, 1122
MunI CAATTG 1 cut(s) 1076
Mva1269I GAATGC 1 cut(s) 840
MvnI CGCG 2 cut(s) 539, 716
MwoI GCNNNNNNNGC 3 cut(s) 759, 776, 998
NdeII GATC 5 cut(s) 172, 238, 735, 1072, 1127
NlaIII CATG 2 cut(s) 195, 1058
NmeAIII GCCGAG 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 368
NsbI TGCGCA 1 cut(s) 1000
NsiI ATGCAT 1 cut(s) 840
PaeR7I CTCGAG 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 750
PctI GAATGC 1 cut(s) 840
PkrI GCNGC 4 cut(s) 538, 772, 895, 991
PleI GAGTC 3 cut(s) 148, 308, 376
PpsI GAGTC 3 cut(s) 148, 308, 376
PshBI ATTAAT 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 359
PspFI CCCAGC 1 cut(s) 947
PspXI VCTCGAGB 1 cut(s) 316
PstNI CAGNNNCTG 2 cut(s) 776, 989
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SacI GAGCTC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 750
SaqAI TTAA 7 cut(s) 35, 108, 414, 666, 1025, 1047, 1122
SatI GCNGC 4 cut(s) 537, 771, 894, 990
Sau3AI GATC 5 cut(s) 172, 238, 735, 1072, 1127
SchI GAGTC 3 cut(s) 148, 308, 376
SduI GDGCHC 1 cut(s) 359
SfaNI GCATC 4 cut(s) 289, 297, 486, 586
Sfr274I CTCGAG 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 316
SmlI CTYRAG 2 cut(s) 316, 352
SmoI CTYRAG 2 cut(s) 316, 352
Sse9I AATT 5 cut(s) 16, 176, 668, 1064, 1076
SsiI CCGC 1 cut(s) 537
SspMI CTAG 5 cut(s) 30, 683, 698, 863, 1139
SstI GAGCTC 1 cut(s) 359
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 467, 579, 985
TaiI ACGT 4 cut(s) 95, 344, 375, 935
TaqI TCGA 6 cut(s) 95, 317, 348, 527, 629, 885
TaqII GACCGA 1 cut(s) 776
TasI AATT 5 cut(s) 16, 176, 668, 1064, 1076
TatI WGTACW 1 cut(s) 117
TauI GCSGC 1 cut(s) 539
Tru1I TTAA 7 cut(s) 35, 108, 414, 666, 1025, 1047, 1122
Tru9I TTAA 7 cut(s) 35, 108, 414, 666, 1025, 1047, 1122
TscAI CASTG 1 cut(s) 398
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 3 cut(s) 770, 893, 989
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 6 cut(s) 126, 219, 455, 585, 898, 1071
TspRI CASTG 1 cut(s) 398
VspI ATTAAT 1 cut(s) 414
XapI RAATTY 3 cut(s) 16, 668, 1064
XbaI TCTAGA 2 cut(s) 29, 862
XhoI CTCGAG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 940
XspI CTAG 5 cut(s) 30, 683, 698, 863, 1139
ZraI GACGTC 1 cut(s) 93
Zsp2I ATGCAT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.