Rroxscaffold_7G00164660

UPF0496 protein At1g20180-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011075
Physical Location & Seq
Reverse (-)
6588828 .. 6590055
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164660.1

Sequence Viewer

Length: 1128 bp
ATGCGCTCCAGGTTGAGATCTATGTTTTCGAAACCAGGCACCAGATCAAGGAAAGTCAATGACCGAGGAGGTGGAGGTAGCTTTTCCAGCAAGCTGAGTGTGAACGAGGAGTACTTGGAAGTTTTCAGAACAAAATCGTATCTAGAAATGTGGGATAGAGTTCATGGATCGGTTCAGCGTCCAACGACAAATACTACTAGACTATCCTCTTCTCCATCGGTTCCTTTCTATATGAATCTCTCCGAAAATCTTCTCGATCCGCGGCAAGAAACCCTCAATGACATGATTAGTGAGGAGGGAATAAACGTGCACCACCTTCTCACTGACTACTTTGAAGCAAGCTTAGAGGCTTGCCATCTATGCGAGTTACTCCTTCGAAGCATCCATCAAACGCACGCAGAGTACCGCAACATCAAGAAGGTGATGAAAAAGCTAAGCCAAAGGAACAATTACACTGACGAGCAATGCCGGGCTATTTTCCGAGAGCTTACAACGTTTGCACGTCTGAAAAACTCGTTGTCGATTATCAGTCCCCTGCAATTCCGTGACATTCAAGATAGCTACTCGGCATTGCTCAATAATTTGACATCGAGACAGAAAAGGATTAGACGCAGGGCAAAGCGGAACAGGATTTTGAAGAAAGTTGGAGGAGTTGGTCTCGTAGTGTCACACAGTGCACTTTTGCTCGCTTTGCTAGTCTTTGCATTTCATAGCATGATTGGACTAGTGGCAGCGCCAGCAATCATGTCTTGTTGTATAGGTTTGTGCAAGAAGAAAATGGAGTCAGGAGACGAGTGGCTCAAGTCGAGTTTCGACCTCGAAATCCATGGGGAGCAACTTGATGTTGCGGCCAAAGGGATTTACATACTGATCAACGATTTCGATACCATGAGTCGGATGGTCAGGCGATTACAGGACGAGGTGGAACACCGCAAAGCTGTTGCAGATTTGTGTGTTAGGAATGGAGTCAATTGCGAAATATTTAATGAGGTTGTGCGAGAATTTCATGTCCATGACTCTAACTTTATGGAGCAGTTGGAGGAGCTTGAAGAGCATGTATACTTGTGTCTGCTGACGATCAACCGATCCAGAAGGCTTGTGCTTCAAGAAATATTCGCAACACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

375

Amino Acids

43.27

Weight (kDa)

8.99

Isoelectric Point (pI)

55.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 74 - 371 4.1e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 1059
AccII CGCG 1 cut(s) 262
AciI CCGC 6 cut(s) 260, 262, 406, 622, 848, 931
AclI AACGTT 1 cut(s) 494
AclWI GGATC 3 cut(s) 175, 251, 1080
AcoI YGGCCR 1 cut(s) 849
AcsI RAATTY 1 cut(s) 1001
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 2 cut(s) 113, 404
AfiI CCNNNNNNNGG 2 cut(s) 48, 894
AgsI TTSAA 5 cut(s) 335, 554, 637, 1049, 1106
AhlI ACTAGT 1 cut(s) 724
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 2 cut(s) 8, 34
AluBI AGCT 8 cut(s) 81, 94, 342, 433, 487, 561, 938, 1045
AluI AGCT 8 cut(s) 81, 94, 342, 433, 487, 561, 938, 1045
Alw21I GWGCWC 2 cut(s) 312, 679
Alw26I GTCTC 3 cut(s) 586, 662, 783
Alw44I GTGCAC 2 cut(s) 308, 675
AlwI GGATC 3 cut(s) 175, 251, 1080
AoxI GGCC 1 cut(s) 849
ApaLI GTGCAC 2 cut(s) 308, 675
ApeKI GCWGC 1 cut(s) 731
ApoI RAATTY 1 cut(s) 1001
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 2 cut(s) 6, 736
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 433
AsuII TTCGAA 2 cut(s) 29, 376
BaeGI GKGCMC 2 cut(s) 312, 679
BanI GGYRCC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 312, 679
BbvI GCAGC 1 cut(s) 743
BccI CCATC 4 cut(s) 223, 363, 393, 892
BciT130I CCWGG 2 cut(s) 10, 36
BclI TGATCA 1 cut(s) 870
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 3 cut(s) 586, 662, 783
BcuI ACTAGT 1 cut(s) 724
BfaI CTAG 4 cut(s) 143, 198, 695, 725
BfoI RGCGCY 1 cut(s) 737
BglII AGATCT 1 cut(s) 17
BisI GCNGC 3 cut(s) 263, 732, 849
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 3 cut(s) 264, 733, 850
BmcAI AGTACT 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 10, 36, 470
BmgBI CACGTC 1 cut(s) 503
BmiI GGNNCC 2 cut(s) 40, 222
BmrFI CCNGG 3 cut(s) 10, 36, 470
BmsI GCATC 1 cut(s) 390
BplI GAGNNNNNCTC 2 cut(s) 642, 674
Bpu1102I GCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 2 cut(s) 29, 376
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 470
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 3 cut(s) 64, 260, 826
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 894
Bse3DI GCAATG 2 cut(s) 470, 569
BseBI CCWGG 2 cut(s) 10, 36
BseDI CCNNGG 3 cut(s) 64, 260, 826
BseGI GGATG 2 cut(s) 381, 903
BseLI CCNNNNNNNGG 2 cut(s) 48, 894
BseMI GCAATG 2 cut(s) 470, 569
BseMII CTCAG 1 cut(s) 86
BseRI GAGGAG 5 cut(s) 81, 122, 308, 663, 1055
BseSI GKGCMC 2 cut(s) 312, 679
BseXI GCAGC 1 cut(s) 743
Bsh1236I CGCG 1 cut(s) 262
BshFI GGCC 1 cut(s) 851
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 312, 679
BsiSI CCGG 1 cut(s) 469
BslFI GGGAC 1 cut(s) 516
BslI CCNNNNNNNGG 2 cut(s) 48, 894
BsmAI GTCTC 3 cut(s) 586, 662, 783
BsmBI CGTCTC 1 cut(s) 783
BsmFI GGGAC 1 cut(s) 516
BsnI GGCC 1 cut(s) 851
Bso31I GGTCTC 1 cut(s) 662
Bsp119I TTCGAA 2 cut(s) 29, 376
Bsp1286I GDGCHC 2 cut(s) 312, 679
Bsp143I GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
Bsp1720I GCTNAGC 1 cut(s) 434
Bsp19I CCATGG 1 cut(s) 826
BspACI CCGC 6 cut(s) 260, 262, 406, 622, 848, 931
BspANI GGCC 1 cut(s) 851
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 40, 222
BspPI GGATC 3 cut(s) 175, 251, 1080
BspQI GCTCTTC 1 cut(s) 1044
BspT104I TTCGAA 2 cut(s) 29, 376
BspT107I GGYRCC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 2 cut(s) 470, 569
BssECI CCNNGG 3 cut(s) 64, 260, 826
BssMI GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
BssNAI GTATAC 1 cut(s) 1060
BssT1I CCWWGG 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 1060
Bst2UI CCWGG 2 cut(s) 10, 36
Bst4CI ACNGT 1 cut(s) 674
Bst6I CTCTTC 2 cut(s) 214, 1044
BstBI TTCGAA 2 cut(s) 29, 376
BstC8I GCNNGC 6 cut(s) 92, 340, 352, 396, 687, 738
BstDEI CTNAG 3 cut(s) 95, 343, 434
BstDSI CCRYGG 2 cut(s) 260, 826
BstF5I GGATG 2 cut(s) 381, 903
BstFNI CGCG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 737
BstHHI GCGC 2 cut(s) 6, 736
BstKTI GATC 7 cut(s) 20, 47, 170, 259, 873, 1080, 1088
BstMAI GTCTC 3 cut(s) 586, 662, 783
BstMBI GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
BstMWI GCNNNNNNNGC 5 cut(s) 87, 360, 691, 737, 1051
BstNI CCWGG 2 cut(s) 10, 36
BstNSI RCATGY 1 cut(s) 1058
BstSCI CCNGG 3 cut(s) 8, 34, 468
BstSLI GKGCMC 2 cut(s) 312, 679
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 743
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BstZ17I GTATAC 1 cut(s) 1060
BsuRI GGCC 1 cut(s) 851
BtgI CCRYGG 2 cut(s) 260, 826
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 2 cut(s) 381, 903
BtsIMutI CAGTG 3 cut(s) 321, 453, 679
Cac8I GCNNGC 6 cut(s) 92, 340, 352, 396, 687, 738
CfoI GCGC 2 cut(s) 6, 736
Cfr42I CCGCGG 1 cut(s) 263
CseI GACGC 2 cut(s) 167, 618
Csp6I GTAC 2 cut(s) 112, 403
CviAII CATG 9 cut(s) 164, 283, 715, 745, 827, 889, 1007, 1013, 1055
CviQI GTAC 2 cut(s) 112, 403
DdeI CTNAG 3 cut(s) 95, 343, 434
DpnI GATC 7 cut(s) 19, 46, 169, 258, 872, 1079, 1087
DpnII GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 849
Eam1104I CTCTTC 2 cut(s) 214, 1044
EarI CTCTTC 2 cut(s) 214, 1044
Eco130I CCWWGG 1 cut(s) 826
Eco31I GGTCTC 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 8, 34
EcoT14I CCWWGG 1 cut(s) 826
ErhI CCWWGG 1 cut(s) 826
Esp3I CGTCTC 1 cut(s) 783
FaeI CATG 9 cut(s) 167, 286, 718, 748, 830, 892, 1010, 1016, 1058
FaqI GGGAC 1 cut(s) 516
FatI CATG 9 cut(s) 163, 282, 714, 744, 826, 888, 1006, 1012, 1054
FbaI TGATCA 1 cut(s) 870
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 3 cut(s) 263, 732, 849
FokI GGATG 2 cut(s) 368, 910
Fsp4HI GCNGC 3 cut(s) 263, 732, 849
FspBI CTAG 4 cut(s) 143, 198, 695, 725
GlaI GCGC 2 cut(s) 5, 735
GluI GCNGC 3 cut(s) 263, 732, 849
HaeII RGCGCY 1 cut(s) 737
HaeIII GGCC 1 cut(s) 851
HapII CCGG 1 cut(s) 469
HgaI GACGC 2 cut(s) 167, 618
HhaI GCGC 2 cut(s) 6, 736
Hin1II CATG 9 cut(s) 167, 286, 718, 748, 830, 892, 1010, 1016, 1058
Hin6I GCGC 2 cut(s) 4, 734
HinP1I GCGC 2 cut(s) 4, 734
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 5 cut(s) 235, 782, 892, 966, 1016
HpaII CCGG 1 cut(s) 469
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 4 cut(s) 103, 310, 677, 1060
Hpy188I TCNGA 5 cut(s) 128, 244, 482, 507, 897
Hpy188III TCNNGA 8 cut(s) 143, 254, 415, 554, 591, 786, 1089, 1106
Hpy8I GTNNAC 4 cut(s) 103, 310, 677, 1060
HpyAV CCTTC 4 cut(s) 326, 383, 412, 1086
HpyCH4III ACNGT 1 cut(s) 674
HpyCH4IV ACGT 3 cut(s) 306, 494, 502
HpyCH4V TGCA 7 cut(s) 310, 500, 538, 677, 704, 768, 944
HpyF10VI GCNNNNNNNGC 5 cut(s) 87, 360, 691, 737, 1051
HpyF3I CTNAG 3 cut(s) 95, 343, 434
HpySE526I ACGT 3 cut(s) 306, 494, 502
Hsp92II CATG 9 cut(s) 167, 286, 718, 748, 830, 892, 1010, 1016, 1058
HspAI GCGC 2 cut(s) 4, 734
Ksp22I TGATCA 1 cut(s) 870
KspI CCGCGG 1 cut(s) 263
Kzo9I GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
LguI GCTCTTC 1 cut(s) 1044
LmnI GCTCC 4 cut(s) 11, 832, 1030, 1042
Lsp1109I GCAGC 1 cut(s) 743
LweI GCATC 1 cut(s) 390
MaeI CTAG 4 cut(s) 143, 198, 695, 725
MaeII ACGT 3 cut(s) 306, 494, 502
MaeIII GTNAC 3 cut(s) 366, 545, 666
MalI GATC 7 cut(s) 19, 46, 169, 258, 872, 1079, 1087
MboI GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
MboII GAAGA 5 cut(s) 201, 242, 649, 784, 1061
MfeI CAATTG 1 cut(s) 970
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 2 cut(s) 312, 679
MluCI AATT 5 cut(s) 448, 539, 580, 970, 1001
MlyI GAGTC 4 cut(s) 791, 901, 975, 1010
MmeI TCCRAC 4 cut(s) 206, 625, 875, 1017
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 2 cut(s) 984, 1126
MslI CAYNNNNRTG 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 1 cut(s) 469
MspR9I CCNGG 3 cut(s) 10, 36, 470
MunI CAATTG 1 cut(s) 970
MvaI CCWGG 2 cut(s) 10, 36
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 5 cut(s) 87, 360, 691, 737, 1051
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 826
NdeII GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
NlaIII CATG 9 cut(s) 167, 286, 718, 748, 830, 892, 1010, 1016, 1058
NlaIV GGNNCC 2 cut(s) 40, 222
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 2 cut(s) 545, 666
NspI RCATGY 1 cut(s) 1058
NspV TTCGAA 2 cut(s) 29, 376
PciSI GCTCTTC 1 cut(s) 1044
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 3 cut(s) 264, 733, 850
PleI GAGTC 4 cut(s) 790, 900, 974, 1010
PpsI GAGTC 4 cut(s) 790, 900, 974, 1010
Psp1406I AACGTT 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 8, 34
PspGI CCWGG 2 cut(s) 8, 34
PspN4I GGNNCC 2 cut(s) 40, 222
PsrI GAACNNNNNNTAC 2 cut(s) 95, 127
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 2 cut(s) 113, 404
RsaNI GTAC 2 cut(s) 112, 403
RseI CAYNNNNRTG 1 cut(s) 1011
SacII CCGCGG 1 cut(s) 263
SapI GCTCTTC 1 cut(s) 1044
SaqAI TTAA 2 cut(s) 984, 1126
SatI GCNGC 3 cut(s) 263, 732, 849
Sau3AI GATC 7 cut(s) 17, 44, 167, 256, 870, 1077, 1085
ScaI AGTACT 1 cut(s) 113
SchI GAGTC 4 cut(s) 791, 901, 975, 1010
ScrFI CCNGG 3 cut(s) 10, 36, 470
SduI GDGCHC 2 cut(s) 312, 679
SfaNI GCATC 1 cut(s) 390
Sfr303I CCGCGG 1 cut(s) 263
SfuI TTCGAA 2 cut(s) 29, 376
SgrBI CCGCGG 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 1011
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
SpeI ACTAGT 1 cut(s) 724
Sse9I AATT 5 cut(s) 448, 539, 580, 970, 1001
SsiI CCGC 6 cut(s) 260, 262, 406, 622, 848, 931
SspI AATATT 2 cut(s) 981, 1113
SspMI CTAG 4 cut(s) 143, 198, 695, 725
StyD4I CCNGG 3 cut(s) 8, 34, 468
StyI CCWWGG 1 cut(s) 826
TaaI ACNGT 1 cut(s) 674
TaiI ACGT 3 cut(s) 309, 497, 505
TaqI TCGA 9 cut(s) 29, 255, 376, 521, 590, 806, 813, 819, 882
TaqII GACCGA 1 cut(s) 78
TasI AATT 5 cut(s) 448, 539, 580, 970, 1001
TatI WGTACW 1 cut(s) 111
TauI GCSGC 2 cut(s) 265, 851
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 2 cut(s) 984, 1126
Tru9I TTAA 2 cut(s) 984, 1126
TscAI CASTG 3 cut(s) 328, 460, 679
TseFI GTSAC 2 cut(s) 545, 666
TseI GCWGC 1 cut(s) 731
Tsp45I GTSAC 2 cut(s) 545, 666
TspDTI ATGAA 5 cut(s) 152, 248, 440, 698, 995
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 3 cut(s) 328, 460, 679
VneI GTGCAC 2 cut(s) 308, 675
XapI RAATTY 1 cut(s) 1001
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 1 cut(s) 1058
XcmI CCANNNNNNNNNTGG 1 cut(s) 895
XmiI GTMKAC 1 cut(s) 1059
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 4 cut(s) 143, 198, 695, 725
ZrmI AGTACT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.