RchiOBHm_Chr6g0303541

UPF0496 protein At1g20180-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011075
Physical Location & Seq
Forward (+)
63250838 .. 63252273
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27270

Sequence Viewer

Length: 1146 bp
ATGAGGAAAAGTATGCGCTCCAGGTTGAGATCTATGTTTTCGAAACCAGGCAGCAGATCAAGGAAAGTCAATGACCGAGGCGGTGGAGGTAGCTTTTCCAGCAAGCTGAGTGTGAACGAGGAGTACTTGGAAGTTTTCAGAACAAAATCGTATCTAGAAATGTGGGATAGAGTTCATGGACATGGATCGGTACAGCGTCCAACAACAAATACTACTAGACTATCCTCTTCTCCATTGGTTCCTTTCTATATGAATCTCTCCGAAAATCTTCTCGATCCGCGGCAAGAAACCCTCAATGACATGATTAGTGAGGAGGGAATAAACGTGCACCACCTTCTCACTGACTACTTTGAATCAAGCTTAGAGGCTTACCATCTATGCGAGTTACTCCTTCGAAGCATCCATCAAACGCACGCAGAGTACCGCAACATCAAGAAGGTGATGAAAAAGCTAAGCCAAAGGAACAATTACACTGACGAGCAATGCCGGGCTATTTTCCGAGAGCTTACGACGTTTGGACATCTGAAAAACTCGTTGTCGATTAGCAGCCCCCTGCAATTCCGTGACATTCACGATAGCTACTCGGCATTGCTCAATAATTTGACATCGAGACAGAAAAAGATTAGACGCAGGGCAAAGCGGAACAGGATTTTGAAGAAAGTTGGAGGAGTTGGTCTCGTAGTGTCTCACAGTGCACTATTGCTCGCTTTGCTAGTCTTTGCATTTCATAGCATGATTGGACTAGTGGCAGCGCCAGCAATCATGTCTTGTTCTATAGGTTTGTGCAAGAAGAAAATGGAGTCAGGAGAGGAGTGTCTCAAGTCGAGTTTCGACCACGAAATCCATGGGGAGCAACTTGATGTTGCGGCCAAAGGGATTTACATACTGATCAACGATTTCGATACCATGAGTCGGATGGTCAGGCGATTACAGGACGAGGTAGAACACCGCAAAGCTGTTGCAGATTTGTGTGTTAGGAATGGAGTCAATTGCGAAATATTTAATGAGGTTGTGCGAGAATTTCATGTCCATGACTCCAACTTTATGGAGCAGTTGGAGGAGCTTGAAGAACATGTATACTTGTGTATGCTGACGATCAACCGATCCAGAAGGCTTGTGCTTCAAGAAATATTCGCAACACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

381

Amino Acids

43.93

Weight (kDa)

9.1

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 83 - 377 5.4e-18 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1077
AccII CGCG 1 cut(s) 280
AciI CCGC 7 cut(s) 81, 278, 280, 424, 640, 866, 949
AclWI GGATC 3 cut(s) 193, 269, 1098
AcoI YGGCCR 1 cut(s) 867
AcsI RAATTY 1 cut(s) 1019
AfaI GTAC 3 cut(s) 125, 192, 422
AfiI CCNNNNNNNGG 1 cut(s) 912
AflIII ACRYGT 1 cut(s) 1072
AgsI TTSAA 4 cut(s) 353, 655, 1067, 1124
AhlI ACTAGT 1 cut(s) 742
AjnI CCWGG 2 cut(s) 20, 46
AluBI AGCT 8 cut(s) 93, 106, 360, 451, 505, 579, 956, 1063
AluI AGCT 8 cut(s) 93, 106, 360, 451, 505, 579, 956, 1063
Alw21I GWGCWC 2 cut(s) 330, 697
Alw26I GTCTC 4 cut(s) 604, 680, 690, 821
Alw44I GTGCAC 2 cut(s) 326, 693
AlwI GGATC 3 cut(s) 193, 269, 1098
AoxI GGCC 1 cut(s) 867
ApaLI GTGCAC 2 cut(s) 326, 693
ApeKI GCWGC 3 cut(s) 51, 546, 749
ApoI RAATTY 1 cut(s) 1019
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 18, 754
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 1 cut(s) 451
AsuII TTCGAA 2 cut(s) 41, 394
BaeGI GKGCMC 2 cut(s) 330, 697
Bbv12I GWGCWC 2 cut(s) 330, 697
BbvI GCAGC 3 cut(s) 63, 558, 761
BccI CCATC 3 cut(s) 381, 411, 910
BciT130I CCWGG 2 cut(s) 22, 48
BclI TGATCA 1 cut(s) 888
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 4 cut(s) 604, 680, 690, 821
BcuI ACTAGT 1 cut(s) 742
BfaI CTAG 4 cut(s) 155, 216, 713, 743
BfmI CTRYAG 1 cut(s) 774
BfoI RGCGCY 1 cut(s) 755
BglII AGATCT 1 cut(s) 29
BisI GCNGC 5 cut(s) 52, 281, 547, 750, 867
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 5 cut(s) 53, 282, 548, 751, 868
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 3 cut(s) 22, 48, 488
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 3 cut(s) 22, 48, 488
BmsI GCATC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 660, 692
BpmI CTGGAG 1 cut(s) 4
Bpu1102I GCTNAGC 1 cut(s) 452
Bpu14I TTCGAA 2 cut(s) 41, 394
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 3 cut(s) 76, 278, 844
Bsc4I CCNNNNNNNGG 1 cut(s) 912
Bse3DI GCAATG 2 cut(s) 488, 587
BseBI CCWGG 2 cut(s) 22, 48
BseDI CCNNGG 3 cut(s) 76, 278, 844
BseGI GGATG 2 cut(s) 399, 921
BseLI CCNNNNNNNGG 1 cut(s) 912
BseMI GCAATG 2 cut(s) 488, 587
BseMII CTCAG 1 cut(s) 98
BseRI GAGGAG 5 cut(s) 134, 326, 681, 824, 1073
BseSI GKGCMC 2 cut(s) 330, 697
BseXI GCAGC 3 cut(s) 63, 558, 761
Bsh1236I CGCG 1 cut(s) 280
BshFI GGCC 1 cut(s) 869
BsiHKAI GWGCWC 2 cut(s) 330, 697
BsiSI CCGG 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 912
BsmAI GTCTC 4 cut(s) 604, 680, 690, 821
BsnI GGCC 1 cut(s) 869
Bso31I GGTCTC 1 cut(s) 680
Bsp119I TTCGAA 2 cut(s) 41, 394
Bsp1286I GDGCHC 2 cut(s) 330, 697
Bsp143I GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
Bsp1720I GCTNAGC 1 cut(s) 452
Bsp19I CCATGG 1 cut(s) 844
BspACI CCGC 7 cut(s) 81, 278, 280, 424, 640, 866, 949
BspANI GGCC 1 cut(s) 869
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 240
BspPI GGATC 3 cut(s) 193, 269, 1098
BspT104I TTCGAA 2 cut(s) 41, 394
BspTNI GGTCTC 1 cut(s) 680
BsrDI GCAATG 2 cut(s) 488, 587
BssECI CCNNGG 3 cut(s) 76, 278, 844
BssMI GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
BssNAI GTATAC 1 cut(s) 1078
BssT1I CCWWGG 1 cut(s) 844
Bst1107I GTATAC 1 cut(s) 1078
Bst2UI CCWGG 2 cut(s) 22, 48
Bst4CI ACNGT 1 cut(s) 692
Bst6I CTCTTC 1 cut(s) 232
BstBI TTCGAA 2 cut(s) 41, 394
BstC8I GCNNGC 4 cut(s) 104, 414, 705, 756
BstDEI CTNAG 3 cut(s) 107, 361, 452
BstDSI CCRYGG 2 cut(s) 278, 844
BstF5I GGATG 2 cut(s) 399, 921
BstFNI CGCG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 755
BstHHI GCGC 2 cut(s) 18, 754
BstKTI GATC 7 cut(s) 32, 59, 188, 277, 891, 1098, 1106
BstMAI GTCTC 4 cut(s) 604, 680, 690, 821
BstMBI GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
BstMWI GCNNNNNNNGC 3 cut(s) 99, 709, 755
BstNI CCWGG 2 cut(s) 22, 48
BstNSI RCATGY 1 cut(s) 1076
BstSCI CCNGG 3 cut(s) 20, 46, 486
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 2 cut(s) 330, 697
BstUI CGCG 1 cut(s) 280
BstV1I GCAGC 3 cut(s) 63, 558, 761
BstX2I RGATCY 1 cut(s) 29
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 1 cut(s) 29
BstZ17I GTATAC 1 cut(s) 1078
BsuRI GGCC 1 cut(s) 869
BtgI CCRYGG 2 cut(s) 278, 844
BtsCI GGATG 2 cut(s) 399, 921
BtsIMutI CAGTG 3 cut(s) 339, 471, 697
Cac8I GCNNGC 4 cut(s) 104, 414, 705, 756
CfoI GCGC 2 cut(s) 18, 754
Cfr42I CCGCGG 1 cut(s) 281
CseI GACGC 2 cut(s) 185, 636
Csp6I GTAC 3 cut(s) 124, 191, 421
CviQI GTAC 3 cut(s) 124, 191, 421
DdeI CTNAG 3 cut(s) 107, 361, 452
DpnI GATC 7 cut(s) 31, 58, 187, 276, 890, 1097, 1105
DpnII GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
EaeI YGGCCR 1 cut(s) 867
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 844
Eco31I GGTCTC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 20, 46
EcoT14I CCWWGG 1 cut(s) 844
ErhI CCWWGG 1 cut(s) 844
FbaI TGATCA 1 cut(s) 888
FblI GTMKAC 1 cut(s) 1077
Fnu4HI GCNGC 5 cut(s) 52, 281, 547, 750, 867
FokI GGATG 2 cut(s) 386, 928
Fsp4HI GCNGC 5 cut(s) 52, 281, 547, 750, 867
FspBI CTAG 4 cut(s) 155, 216, 713, 743
GlaI GCGC 2 cut(s) 17, 753
GluI GCNGC 5 cut(s) 52, 281, 547, 750, 867
GsuI CTGGAG 1 cut(s) 4
HaeII RGCGCY 1 cut(s) 755
HaeIII GGCC 1 cut(s) 869
HapII CCGG 1 cut(s) 487
HgaI GACGC 2 cut(s) 185, 636
HhaI GCGC 2 cut(s) 18, 754
Hin6I GCGC 2 cut(s) 16, 752
HinP1I GCGC 2 cut(s) 16, 752
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 6 cut(s) 253, 353, 800, 910, 984, 1034
HpaII CCGG 1 cut(s) 487
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 4 cut(s) 115, 328, 695, 1078
Hpy188I TCNGA 5 cut(s) 140, 262, 500, 525, 915
Hpy188III TCNNGA 8 cut(s) 155, 272, 433, 572, 609, 804, 1107, 1124
Hpy8I GTNNAC 4 cut(s) 115, 328, 695, 1078
Hpy99I CGWCG 1 cut(s) 514
HpyAV CCTTC 4 cut(s) 344, 401, 430, 1104
HpyCH4III ACNGT 1 cut(s) 692
HpyCH4IV ACGT 2 cut(s) 324, 512
HpyCH4V TGCA 6 cut(s) 328, 556, 695, 722, 786, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 709, 755
HpyF3I CTNAG 3 cut(s) 107, 361, 452
HpySE526I ACGT 2 cut(s) 324, 512
HspAI GCGC 2 cut(s) 16, 752
Ksp22I TGATCA 1 cut(s) 888
KspI CCGCGG 1 cut(s) 281
Kzo9I GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
LmnI GCTCC 4 cut(s) 23, 850, 1048, 1060
Lsp1109I GCAGC 3 cut(s) 63, 558, 761
LweI GCATC 1 cut(s) 408
MaeI CTAG 4 cut(s) 155, 216, 713, 743
MaeII ACGT 2 cut(s) 324, 512
MaeIII GTNAC 2 cut(s) 384, 563
MalI GATC 7 cut(s) 31, 58, 187, 276, 890, 1097, 1105
MboI GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
MboII GAAGA 5 cut(s) 219, 260, 667, 802, 1079
MfeI CAATTG 1 cut(s) 988
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 330, 697
MluCI AATT 5 cut(s) 466, 557, 598, 988, 1019
MlyI GAGTC 4 cut(s) 809, 919, 993, 1028
MmeI TCCRAC 5 cut(s) 224, 643, 893, 1035, 1062
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 1 cut(s) 1002
MslI CAYNNNNRTG 2 cut(s) 180, 1029
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 1 cut(s) 487
MspR9I CCNGG 3 cut(s) 22, 48, 488
MunI CAATTG 1 cut(s) 988
MvaI CCWGG 2 cut(s) 22, 48
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 3 cut(s) 99, 709, 755
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 1 cut(s) 844
NdeII GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
NlaIV GGNNCC 1 cut(s) 240
NmeAIII GCCGAG 1 cut(s) 563
NmuCI GTSAC 1 cut(s) 563
NspI RCATGY 1 cut(s) 1076
NspV TTCGAA 2 cut(s) 41, 394
PciI ACATGT 1 cut(s) 1072
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 2 cut(s) 253, 353
PkrI GCNGC 5 cut(s) 53, 282, 548, 751, 868
PleI GAGTC 4 cut(s) 808, 918, 992, 1028
PpsI GAGTC 4 cut(s) 808, 918, 992, 1028
PscI ACATGT 1 cut(s) 1072
Psp6I CCWGG 2 cut(s) 20, 46
PspGI CCWGG 2 cut(s) 20, 46
PspN4I GGNNCC 1 cut(s) 240
PsrI GAACNNNNNNTAC 2 cut(s) 107, 139
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 3 cut(s) 125, 192, 422
RsaNI GTAC 3 cut(s) 124, 191, 421
RseI CAYNNNNRTG 2 cut(s) 180, 1029
SacII CCGCGG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 1002
SatI GCNGC 5 cut(s) 52, 281, 547, 750, 867
Sau3AI GATC 7 cut(s) 29, 56, 185, 274, 888, 1095, 1103
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 4 cut(s) 809, 919, 993, 1028
ScrFI CCNGG 3 cut(s) 22, 48, 488
SduI GDGCHC 2 cut(s) 330, 697
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 1 cut(s) 774
Sfr303I CCGCGG 1 cut(s) 281
SfuI TTCGAA 2 cut(s) 41, 394
SgrBI CCGCGG 1 cut(s) 281
SmiMI CAYNNNNRTG 2 cut(s) 180, 1029
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
SpeI ACTAGT 1 cut(s) 742
Sse9I AATT 5 cut(s) 466, 557, 598, 988, 1019
SsiI CCGC 7 cut(s) 81, 278, 280, 424, 640, 866, 949
SspI AATATT 2 cut(s) 999, 1131
SspMI CTAG 4 cut(s) 155, 216, 713, 743
StyD4I CCNGG 3 cut(s) 20, 46, 486
StyI CCWWGG 1 cut(s) 844
TaaI ACNGT 1 cut(s) 692
TaiI ACGT 2 cut(s) 327, 515
TaqI TCGA 8 cut(s) 41, 273, 394, 539, 608, 824, 831, 900
TaqII GACCGA 1 cut(s) 90
TasI AATT 5 cut(s) 466, 557, 598, 988, 1019
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 283, 869
TfiI GAWTC 2 cut(s) 253, 353
Tru1I TTAA 1 cut(s) 1002
Tru9I TTAA 1 cut(s) 1002
TscAI CASTG 3 cut(s) 346, 478, 697
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 3 cut(s) 51, 546, 749
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 5 cut(s) 164, 266, 458, 716, 1013
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 3 cut(s) 346, 478, 697
VneI GTGCAC 2 cut(s) 326, 693
XapI RAATTY 1 cut(s) 1019
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 1076
XcmI CCANNNNNNNNNTGG 2 cut(s) 842, 913
XmiI GTMKAC 1 cut(s) 1077
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 4 cut(s) 155, 216, 713, 743
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.