MD03G1275300.v1.1

UPF0496 protein At1g20180-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011075
Physical Location & Seq
Forward (+)
35826551 .. 35827513
963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1275300.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGTGTTGGACCGCTCCGATATTTTACTCGAACCACGGCAAGAAACCCTTAAAAACATCATCGATGAGGAGGGCTTAAATCTCCACCACCTTCTCACCGACTACTTCGAAGCCAGCTTAGACGCCTGCCACATATGTGACGTGCTCCTGCATAGCATCCACCAAACCCGTGCCGAAAACCGACACACGAAGAAGCTCGTATTAAAACTCAGCCAAAGAGTACACCTACTTTATAATGATAATATCGACATTACTGATGATGAACAATGCCTGGAAATTTTCGGGGAGCTTGGCACATTTGCCCGAGCTAAAAATCCGTTGTCGATAATCAGCCCTGGGCCGTTCCGTCACATTCATGATAGCTACGTGGCGTTATTCGATAGGCTGATTTTAAAGCACAAAAAGGTGCGAAGGGCCTCAAAGCGTAACAGAATATGCAAAAAGGTTGGAGGGGTTGGTCTGGTGGTGTCACATACCGCGCTTTTGATCACGTTGCTTGTGTTTGCAATCCACAGCATGATTGGAATCGTGGTGGCGCCGGCGCTCATAGCCTGCTCGTTGGGTTTGTGCGCGAGAAAAATGGACTCCGCAGCGGAGTGGTTGAAGACTAGGTTTCCCGAGAGCCACGGGCAGCAACTTGACGTGGCGGCGAAAGGGGTTTATATTTTGATCAATGATTTCGATACAATGAGCCGGATGGTGAGGCGGTTGTACGACGAGGTGGAGCACAGGAAAGCTGTCGCCAGAATGTGTGTTAGCAGTGTGAGGAACATAATTAGTACTAATAATGGTGGATCATCTTCAAACTGTGACATATTTAAGAAGGTGGTGAGGGAATTTCATGCGAGTGAGGATAGCTTTGTGGAGCAGCTAGAGGAGCTTGAAGAACATATTTACTTGTGCTTGCTCACCATAAACAGGTCGAGAAGGCTTGTGATACAAGAAATATTGGCAAAGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

321

Amino Acids

36.38

Weight (kDa)

8.07

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 17 - 316 2.3e-08 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 15
AccII CGCG 2 cut(s) 479, 572
AciI CCGC 6 cut(s) 13, 477, 588, 593, 647, 706
AclWI GGATC 1 cut(s) 802
AcsI RAATTY 2 cut(s) 276, 836
AcyI GRCGYC 2 cut(s) 123, 536
AfaI GTAC 3 cut(s) 222, 713, 781
AfiI CCNNNNNNNGG 1 cut(s) 918
AgsI TTSAA 3 cut(s) 604, 804, 884
AjiI CACGTC 2 cut(s) 142, 643
AjnI CCWGG 2 cut(s) 270, 334
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 9 cut(s) 117, 196, 289, 308, 363, 737, 858, 871, 880
AluI AGCT 9 cut(s) 117, 196, 289, 308, 363, 737, 858, 871, 880
Alw21I GWGCWC 2 cut(s) 147, 729
AlwI GGATC 1 cut(s) 802
Ama87I CYCGRG 2 cut(s) 303, 617
AoxI GGCC 2 cut(s) 338, 414
ApeKI GCWGC 3 cut(s) 590, 631, 868
ApoI RAATTY 2 cut(s) 276, 836
AspLEI GCGC 4 cut(s) 481, 538, 544, 572
AspS9I GGNCC 3 cut(s) 10, 338, 414
AsuHPI GGTGA 4 cut(s) 88, 712, 841, 901
AsuII TTCGAA 1 cut(s) 108
AvaI CYCGRG 2 cut(s) 303, 617
AvaII GGWCC 1 cut(s) 10
BanI GGYRCC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 2 cut(s) 147, 729
BbvI GCAGC 3 cut(s) 602, 643, 880
BccI CCATC 1 cut(s) 691
BceAI ACGGC 2 cut(s) 53, 325
BciT130I CCWGG 2 cut(s) 272, 336
BclI TGATCA 2 cut(s) 486, 669
BfaI CTAG 2 cut(s) 609, 872
BfoI RGCGCY 2 cut(s) 539, 545
BisI GCNGC 4 cut(s) 591, 632, 648, 869
BlsI GCNGC 4 cut(s) 592, 633, 649, 870
BmcAI AGTACT 1 cut(s) 781
Bme1390I CCNGG 2 cut(s) 272, 336
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 2 cut(s) 303, 617
BmgBI CACGTC 2 cut(s) 142, 643
BmgT120I GGNCC 3 cut(s) 10, 338, 414
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 2 cut(s) 272, 336
BmsI GCATC 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 611
Bpu14I TTCGAA 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 367
BsaBI GATNNNNATC 1 cut(s) 524
BsaHI GRCGYC 2 cut(s) 123, 536
BsaJI CCNNGG 4 cut(s) 35, 334, 335, 625
Bsc4I CCNNNNNNNGG 1 cut(s) 918
Bse118I RCCGGY 1 cut(s) 538
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 2 cut(s) 272, 336
BseCI ATCGAT 1 cut(s) 63
BseDI CCNNGG 4 cut(s) 35, 334, 335, 625
BseGI GGATG 2 cut(s) 156, 702
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 1 cut(s) 918
BseMII CTCAG 1 cut(s) 223
BseRI GAGGAG 2 cut(s) 83, 890
BseXI GCAGC 3 cut(s) 602, 643, 880
Bsh1236I CGCG 2 cut(s) 479, 572
BshFI GGCC 2 cut(s) 340, 416
BshNI GGYRCC 1 cut(s) 535
BshVI ATCGAT 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 147, 729
BsiHKCI CYCGRG 2 cut(s) 303, 617
BsiSI CCGG 2 cut(s) 539, 694
BslI CCNNNNNNNGG 1 cut(s) 918
BsnI GGCC 2 cut(s) 340, 416
BsoBI CYCGRG 2 cut(s) 303, 617
Bsp119I TTCGAA 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 147, 729
Bsp143I GATC 3 cut(s) 486, 669, 794
BspACI CCGC 6 cut(s) 13, 477, 588, 593, 647, 706
BspANI GGCC 2 cut(s) 340, 416
BspCNI CTCAG 1 cut(s) 222
BspDI ATCGAT 1 cut(s) 63
BspFNI CGCG 2 cut(s) 479, 572
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 537
BspPI GGATC 1 cut(s) 802
BspT104I TTCGAA 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 538
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 4 cut(s) 35, 334, 335, 625
BssMI GATC 3 cut(s) 486, 669, 794
BssNI GRCGYC 2 cut(s) 123, 536
Bst2UI CCWGG 2 cut(s) 272, 336
Bst4CI ACNGT 1 cut(s) 809
BstACI GRCGYC 2 cut(s) 123, 536
BstBAI YACGTR 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 108
BstC8I GCNNGC 5 cut(s) 115, 127, 540, 553, 905
BstDEI CTNAG 2 cut(s) 118, 209
BstDSI CCRYGG 2 cut(s) 35, 625
BstF5I GGATG 2 cut(s) 156, 702
BstFNI CGCG 2 cut(s) 479, 572
BstH2I RGCGCY 2 cut(s) 539, 545
BstHHI GCGC 4 cut(s) 481, 538, 544, 572
BstKTI GATC 3 cut(s) 489, 672, 797
BstMBI GATC 3 cut(s) 486, 669, 794
BstMWI GCNNNNNNNGC 2 cut(s) 548, 877
BstNI CCWGG 2 cut(s) 272, 336
BstSCI CCNGG 2 cut(s) 270, 334
BstUI CGCG 2 cut(s) 479, 572
BstV1I GCAGC 3 cut(s) 602, 643, 880
BstV2I GAAGAC 1 cut(s) 611
Bsu15I ATCGAT 1 cut(s) 63
BsuRI GGCC 2 cut(s) 340, 416
BsuTUI ATCGAT 1 cut(s) 63
BtgI CCRYGG 2 cut(s) 35, 625
BtrI CACGTC 2 cut(s) 142, 643
BtsCI GGATG 2 cut(s) 156, 702
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 1 cut(s) 766
Cac8I GCNNGC 5 cut(s) 115, 127, 540, 553, 905
CciI TCATGA 1 cut(s) 355
CfoI GCGC 4 cut(s) 481, 538, 544, 572
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 3 cut(s) 10, 338, 414
ClaI ATCGAT 1 cut(s) 63
CseI GACGC 1 cut(s) 131
Csp6I GTAC 3 cut(s) 221, 712, 780
CviAII CATG 3 cut(s) 356, 517, 842
CviQI GTAC 3 cut(s) 221, 712, 780
DdeI CTNAG 2 cut(s) 118, 209
DinI GGCGCC 1 cut(s) 537
DpnI GATC 3 cut(s) 488, 671, 796
DpnII GATC 3 cut(s) 486, 669, 794
DraI TTTAAA 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 10
Eco88I CYCGRG 2 cut(s) 303, 617
EcoO109I RGGNCCY 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 270, 334
EgeI GGCGCC 1 cut(s) 537
EheI GGCGCC 1 cut(s) 537
FaeI CATG 3 cut(s) 359, 520, 845
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FatI CATG 3 cut(s) 355, 516, 841
FauNDI CATATG 1 cut(s) 134
FbaI TGATCA 2 cut(s) 486, 669
Fnu4HI GCNGC 4 cut(s) 591, 632, 648, 869
FokI GGATG 2 cut(s) 143, 709
Fsp4HI GCNGC 4 cut(s) 591, 632, 648, 869
FspBI CTAG 2 cut(s) 609, 872
GlaI GCGC 4 cut(s) 480, 537, 543, 571
GluI GCNGC 4 cut(s) 591, 632, 648, 869
HaeII RGCGCY 2 cut(s) 539, 545
HaeIII GGCC 2 cut(s) 340, 416
HapII CCGG 2 cut(s) 539, 694
HgaI GACGC 1 cut(s) 131
HhaI GCGC 4 cut(s) 481, 538, 544, 572
Hin1I GRCGYC 2 cut(s) 123, 536
Hin1II CATG 3 cut(s) 359, 520, 845
Hin6I GCGC 4 cut(s) 479, 536, 542, 570
HinP1I GCGC 4 cut(s) 479, 536, 542, 570
HinfI GANTC 2 cut(s) 525, 584
HpaII CCGG 2 cut(s) 539, 694
HphI GGTGA 4 cut(s) 88, 712, 841, 901
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 3 cut(s) 356, 617, 924
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 4 cut(s) 101, 405, 817, 921
HpyCH4III ACNGT 1 cut(s) 809
HpyCH4IV ACGT 4 cut(s) 141, 366, 491, 642
HpyCH4V TGCA 3 cut(s) 151, 438, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 548, 877
HpyF3I CTNAG 2 cut(s) 118, 209
HpySE526I ACGT 4 cut(s) 141, 366, 491, 642
Hsp92I GRCGYC 2 cut(s) 123, 536
Hsp92II CATG 3 cut(s) 359, 520, 845
HspAI GCGC 4 cut(s) 479, 536, 542, 570
KasI GGCGCC 1 cut(s) 535
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Ksp22I TGATCA 2 cut(s) 486, 669
Kzo9I GATC 3 cut(s) 486, 669, 794
LmnI GCTCC 6 cut(s) 20, 150, 286, 724, 865, 877
Lsp1109I GCAGC 3 cut(s) 602, 643, 880
LweI GCATC 1 cut(s) 165
MaeI CTAG 2 cut(s) 609, 872
MaeII ACGT 4 cut(s) 141, 366, 491, 642
MaeIII GTNAC 5 cut(s) 137, 347, 425, 468, 809
MalI GATC 3 cut(s) 488, 671, 796
MbiI CCGCTC 1 cut(s) 15
MboI GATC 3 cut(s) 486, 669, 794
MboII GAAGA 4 cut(s) 202, 616, 792, 896
MhlI GDGCHC 2 cut(s) 147, 729
MluCI AATT 3 cut(s) 276, 774, 836
Mly113I GGCGCC 1 cut(s) 536
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 427
MreI CGCCGGCG 1 cut(s) 538
MroNI GCCGGC 1 cut(s) 538
MseI TTAA 5 cut(s) 51, 77, 203, 392, 819
MslI CAYNNNNRTG 3 cut(s) 135, 354, 846
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 2 cut(s) 539, 694
MspR9I CCNGG 2 cut(s) 272, 336
MvaI CCWGG 2 cut(s) 272, 336
MvnI CGCG 2 cut(s) 479, 572
MwoI GCNNNNNNNGC 2 cut(s) 548, 877
NaeI GCCGGC 1 cut(s) 540
NarI GGCGCC 1 cut(s) 536
NdeI CATATG 1 cut(s) 134
NdeII GATC 3 cut(s) 486, 669, 794
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 3 cut(s) 359, 520, 845
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 4 cut(s) 137, 347, 468, 809
NspV TTCGAA 1 cut(s) 108
OliI CACNNNNGTG 1 cut(s) 135
PagI TCATGA 1 cut(s) 355
PasI CCCWGGG 1 cut(s) 335
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 1 cut(s) 525
PkrI GCNGC 4 cut(s) 592, 633, 649, 870
PleI GAGTC 1 cut(s) 578
PluTI GGCGCC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 578
Ppu21I YACGTR 1 cut(s) 367
PsiI TTATAA 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 270, 334
PspGI CCWGG 2 cut(s) 270, 334
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 3 cut(s) 10, 338, 414
RsaI GTAC 3 cut(s) 222, 713, 781
RsaNI GTAC 3 cut(s) 221, 712, 780
RseI CAYNNNNRTG 3 cut(s) 135, 354, 846
SaqAI TTAA 5 cut(s) 51, 77, 203, 392, 819
SatI GCNGC 4 cut(s) 591, 632, 648, 869
Sau3AI GATC 3 cut(s) 486, 669, 794
Sau96I GGNCC 3 cut(s) 10, 338, 414
ScaI AGTACT 1 cut(s) 781
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 2 cut(s) 272, 336
SduI GDGCHC 2 cut(s) 147, 729
SfaNI GCATC 1 cut(s) 165
SfoI GGCGCC 1 cut(s) 537
SfuI TTCGAA 1 cut(s) 108
SgrAI CRCCGGYG 1 cut(s) 538
SinI GGWCC 1 cut(s) 10
SmiMI CAYNNNNRTG 3 cut(s) 135, 354, 846
Sse9I AATT 3 cut(s) 276, 774, 836
SsiI CCGC 6 cut(s) 13, 477, 588, 593, 647, 706
SspDI GGCGCC 1 cut(s) 535
SspI AATATT 1 cut(s) 948
SspMI CTAG 2 cut(s) 609, 872
StyD4I CCNGG 2 cut(s) 270, 334
TaaI ACNGT 1 cut(s) 809
TaiI ACGT 4 cut(s) 144, 369, 494, 645
TaqI TCGA 8 cut(s) 30, 63, 108, 246, 323, 378, 681, 923
TasI AATT 3 cut(s) 276, 774, 836
TatI WGTACW 2 cut(s) 220, 779
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 1 cut(s) 525
Tru1I TTAA 5 cut(s) 51, 77, 203, 392, 819
Tru9I TTAA 5 cut(s) 51, 77, 203, 392, 819
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 4 cut(s) 137, 347, 468, 809
TseI GCWGC 3 cut(s) 590, 631, 868
Tsp45I GTSAC 4 cut(s) 137, 347, 468, 809
TspDTI ATGAA 3 cut(s) 276, 344, 830
TspGWI ACGGA 2 cut(s) 306, 335
TspRI CASTG 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 10
XapI RAATTY 2 cut(s) 276, 836
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XspI CTAG 2 cut(s) 609, 872
ZrmI AGTACT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.