Rw6G037780

UPF0496 protein At1g20180-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011075
Physical Location & Seq
Forward (+)
62059164 .. 62070880
11717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037780.1

Sequence Viewer

Length: 1140 bp
ATGTCCAAGATGAGGAAAAACCTGCGCTCCAGGTTGAGATATATGTTTTCGAAACCAGGTGAGAAAGTCAATGATCGAGGCGGTGGAGGTAGCTTTTCCAGCAAGCTGAGTGTGAACGAGGAGTACTTGGAAGTTTTCAGAACAAAATCGTATCTAGAAATGTGGGATAGAGTTCATGGATCGGTACAGCGTCCAACGACAAATACTACTAGACTATCCTCTTCTCCATCGGTTCCTTTCTATATGAATCTCTCCGAAAATCTTCTCGATCCGCGGCAAGAAACCCTCAATGACATGATTAGTGAGGAGGGAATAAACGTGCACCACCTTCTCACTGACTACTTTGAAGCAAGCTTAGAGGCTTGCCATCTATGCGAGTTACTCCTTCGAAGCATCCATCAAACGCACGCAGAGTACCGCAACATCAAGAAGGTGATGAAAAAGCTAAGCCAAAGGAACAATTACACTGACGAGCAATGCCGGGCTATTTTCCGAGAGCTTACAACGTTTGGACGTCTGAAAAACTCGTTGTCGATTATCAGCCCCCTGCAATTCCGTGGCATTCACGATAGCTACTCGGCATTGCTCAATAATTTGACATCAATACAGAAAAAGATTAGACGCAGGGCAAAGCGGAACAGGATTTTGAAGAAAGTTGGAGGAGTTGGTCTCGTAGTGTCACACAGTGCACTTTTGCTCGCTTTGCTAGTCTTTGCATTTCATAGCATGATTGGACTAGTGGCAGCGCCAGCAATCATGTCTTGTTCTATAGGTTTGTGCAAGAAGAAAATGGAGTCAGGAGAGGAGTGGCTCAAGTCGAGTTTCGACCACGAAATCCATGGGGAGCAACTTGATGTTGCGGCCAAAGGGATTTACATACTGATCAACGATTTCGATACCATGAGTCGGATGGTCAGGCGATTACAGGACGAGGTAGAACACCGCAAAGCTGTTGCAGATTTGTGTGTTAGGAATGGAGTCAATTGCGAAATATTTAATGAGGTTGTACGAGAATTTCATGTCCATGACTCCAACTTTATGGAGCAGTTGGAGGAGCTTGAAGAACATGTATACTTGTGTATGCTGACGATCAACCGATCCAGAAGGCTTGTGCTTCAAGAAATATTCGCAACACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

379

Amino Acids

43.74

Weight (kDa)

9.01

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 78 - 375 3e-16 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 517
Acc36I ACCTGC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 1071
AccII CGCG 1 cut(s) 274
AciI CCGC 7 cut(s) 81, 272, 274, 418, 634, 860, 943
AclI AACGTT 1 cut(s) 506
AclWI GGATC 3 cut(s) 187, 263, 1092
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 1 cut(s) 1013
AcyI GRCGYC 1 cut(s) 514
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 4 cut(s) 125, 186, 416, 1008
AfiI CCNNNNNNNGG 2 cut(s) 12, 906
AflIII ACRYGT 1 cut(s) 1066
AgsI TTSAA 4 cut(s) 347, 649, 1061, 1118
AhlI ACTAGT 1 cut(s) 736
AjnI CCWGG 2 cut(s) 29, 55
AluBI AGCT 8 cut(s) 93, 106, 354, 445, 499, 573, 950, 1057
AluI AGCT 8 cut(s) 93, 106, 354, 445, 499, 573, 950, 1057
Alw21I GWGCWC 2 cut(s) 324, 691
Alw26I GTCTC 1 cut(s) 674
Alw44I GTGCAC 2 cut(s) 320, 687
AlwI GGATC 3 cut(s) 187, 263, 1092
AoxI GGCC 1 cut(s) 861
ApaLI GTGCAC 2 cut(s) 320, 687
ApeKI GCWGC 1 cut(s) 743
ApoI RAATTY 1 cut(s) 1013
Asp700I GAANNNNTTC 1 cut(s) 261
AspLEI GCGC 2 cut(s) 27, 748
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 71, 445
AsuII TTCGAA 2 cut(s) 50, 388
BaeGI GKGCMC 2 cut(s) 324, 691
Bbv12I GWGCWC 2 cut(s) 324, 691
BbvI GCAGC 1 cut(s) 755
BccI CCATC 4 cut(s) 235, 375, 405, 904
BciT130I CCWGG 2 cut(s) 31, 57
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 1 cut(s) 674
BcuI ACTAGT 1 cut(s) 736
BfaI CTAG 4 cut(s) 155, 210, 707, 737
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 30
BisI GCNGC 3 cut(s) 275, 744, 861
BlpI GCTNAGC 1 cut(s) 446
BlsI GCNGC 3 cut(s) 276, 745, 862
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 3 cut(s) 31, 57, 482
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 3 cut(s) 31, 57, 482
BmsI GCATC 1 cut(s) 402
BplI GAGNNNNNCTC 2 cut(s) 654, 686
BpmI CTGGAG 1 cut(s) 13
Bpu1102I GCTNAGC 1 cut(s) 446
Bpu14I TTCGAA 2 cut(s) 50, 388
BpuEI CTTGAG 1 cut(s) 797
BpuMI CCSGG 1 cut(s) 482
BsaHI GRCGYC 1 cut(s) 514
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 3 cut(s) 272, 556, 838
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 906
Bse3DI GCAATG 2 cut(s) 482, 581
BseBI CCWGG 2 cut(s) 31, 57
BseDI CCNNGG 3 cut(s) 272, 556, 838
BseGI GGATG 2 cut(s) 393, 915
BseLI CCNNNNNNNGG 2 cut(s) 12, 906
BseMI GCAATG 2 cut(s) 482, 581
BseMII CTCAG 1 cut(s) 98
BseRI GAGGAG 5 cut(s) 134, 320, 675, 818, 1067
BseSI GKGCMC 2 cut(s) 324, 691
BseXI GCAGC 1 cut(s) 755
Bsh1236I CGCG 1 cut(s) 274
BshFI GGCC 1 cut(s) 863
BsiHKAI GWGCWC 2 cut(s) 324, 691
BsiSI CCGG 1 cut(s) 481
BslI CCNNNNNNNGG 2 cut(s) 12, 906
BsmAI GTCTC 1 cut(s) 674
BsmI GAATGC 1 cut(s) 561
BsnI GGCC 1 cut(s) 863
Bso31I GGTCTC 1 cut(s) 674
Bsp119I TTCGAA 2 cut(s) 50, 388
Bsp1286I GDGCHC 2 cut(s) 324, 691
Bsp143I GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
Bsp1720I GCTNAGC 1 cut(s) 446
Bsp19I CCATGG 1 cut(s) 838
BspACI CCGC 7 cut(s) 81, 272, 274, 418, 634, 860, 943
BspANI GGCC 1 cut(s) 863
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 234
BspMI ACCTGC 1 cut(s) 30
BspPI GGATC 3 cut(s) 187, 263, 1092
BspT104I TTCGAA 2 cut(s) 50, 388
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 2 cut(s) 482, 581
BssECI CCNNGG 3 cut(s) 272, 556, 838
BssMI GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
BssNAI GTATAC 1 cut(s) 1072
BssNI GRCGYC 1 cut(s) 514
BssT1I CCWWGG 1 cut(s) 838
Bst1107I GTATAC 1 cut(s) 1072
Bst2UI CCWGG 2 cut(s) 31, 57
Bst4CI ACNGT 1 cut(s) 686
Bst6I CTCTTC 1 cut(s) 226
BstACI GRCGYC 1 cut(s) 514
BstBI TTCGAA 2 cut(s) 50, 388
BstC8I GCNNGC 6 cut(s) 104, 352, 364, 408, 699, 750
BstDEI CTNAG 3 cut(s) 107, 355, 446
BstDSI CCRYGG 3 cut(s) 272, 556, 838
BstF5I GGATG 2 cut(s) 393, 915
BstFNI CGCG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 749
BstHHI GCGC 2 cut(s) 27, 748
BstKTI GATC 6 cut(s) 76, 182, 271, 885, 1092, 1100
BstMAI GTCTC 1 cut(s) 674
BstMBI GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
BstMWI GCNNNNNNNGC 4 cut(s) 99, 372, 703, 749
BstNI CCWGG 2 cut(s) 31, 57
BstNSI RCATGY 1 cut(s) 1070
BstSCI CCNGG 3 cut(s) 29, 55, 480
BstSFI CTRYAG 1 cut(s) 768
BstSLI GKGCMC 2 cut(s) 324, 691
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 755
BstXI CCANNNNNNTGG 1 cut(s) 1039
BstZ17I GTATAC 1 cut(s) 1072
BsuRI GGCC 1 cut(s) 863
BtgI CCRYGG 3 cut(s) 272, 556, 838
BtsCI GGATG 2 cut(s) 393, 915
BtsIMutI CAGTG 3 cut(s) 333, 465, 691
BveI ACCTGC 1 cut(s) 30
Cac8I GCNNGC 6 cut(s) 104, 352, 364, 408, 699, 750
CfoI GCGC 2 cut(s) 27, 748
Cfr42I CCGCGG 1 cut(s) 275
CseI GACGC 2 cut(s) 179, 630
CsiI ACCWGGT 1 cut(s) 55
Csp6I GTAC 4 cut(s) 124, 185, 415, 1007
CviAII CATG 9 cut(s) 176, 295, 727, 757, 839, 901, 1019, 1025, 1067
CviQI GTAC 4 cut(s) 124, 185, 415, 1007
DdeI CTNAG 3 cut(s) 107, 355, 446
DpnI GATC 6 cut(s) 75, 181, 270, 884, 1091, 1099
DpnII GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
DraIII CACNNNGTG 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 861
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 29, 55
EcoT14I CCWWGG 1 cut(s) 838
ErhI CCWWGG 1 cut(s) 838
FaeI CATG 9 cut(s) 179, 298, 730, 760, 842, 904, 1022, 1028, 1070
FatI CATG 9 cut(s) 175, 294, 726, 756, 838, 900, 1018, 1024, 1066
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 1 cut(s) 1071
Fnu4HI GCNGC 3 cut(s) 275, 744, 861
FokI GGATG 2 cut(s) 380, 922
Fsp4HI GCNGC 3 cut(s) 275, 744, 861
FspBI CTAG 4 cut(s) 155, 210, 707, 737
GlaI GCGC 2 cut(s) 26, 747
GluI GCNGC 3 cut(s) 275, 744, 861
GsuI CTGGAG 1 cut(s) 13
HaeII RGCGCY 1 cut(s) 749
HaeIII GGCC 1 cut(s) 863
HapII CCGG 1 cut(s) 481
HgaI GACGC 2 cut(s) 179, 630
HhaI GCGC 2 cut(s) 27, 748
Hin1I GRCGYC 1 cut(s) 514
Hin1II CATG 9 cut(s) 179, 298, 730, 760, 842, 904, 1022, 1028, 1070
Hin6I GCGC 2 cut(s) 25, 746
HinP1I GCGC 2 cut(s) 25, 746
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 5 cut(s) 247, 794, 904, 978, 1028
HpaII CCGG 1 cut(s) 481
HphI GGTGA 2 cut(s) 71, 445
Hpy166II GTNNAC 4 cut(s) 115, 322, 689, 1072
Hpy188I TCNGA 5 cut(s) 140, 256, 494, 519, 909
Hpy188III TCNNGA 7 cut(s) 155, 266, 427, 566, 798, 1101, 1118
Hpy8I GTNNAC 4 cut(s) 115, 322, 689, 1072
HpyAV CCTTC 4 cut(s) 338, 395, 424, 1098
HpyCH4III ACNGT 1 cut(s) 686
HpyCH4IV ACGT 3 cut(s) 318, 506, 514
HpyCH4V TGCA 6 cut(s) 322, 550, 689, 716, 780, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 372, 703, 749
HpyF3I CTNAG 3 cut(s) 107, 355, 446
HpySE526I ACGT 3 cut(s) 318, 506, 514
Hsp92I GRCGYC 1 cut(s) 514
Hsp92II CATG 9 cut(s) 179, 298, 730, 760, 842, 904, 1022, 1028, 1070
HspAI GCGC 2 cut(s) 25, 746
Ksp22I TGATCA 1 cut(s) 882
KspI CCGCGG 1 cut(s) 275
Kzo9I GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
LmnI GCTCC 4 cut(s) 32, 844, 1042, 1054
Lsp1109I GCAGC 1 cut(s) 755
LweI GCATC 1 cut(s) 402
MabI ACCWGGT 1 cut(s) 55
MaeI CTAG 4 cut(s) 155, 210, 707, 737
MaeII ACGT 3 cut(s) 318, 506, 514
MaeIII GTNAC 2 cut(s) 378, 678
MalI GATC 6 cut(s) 75, 181, 270, 884, 1091, 1099
MboI GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
MboII GAAGA 5 cut(s) 213, 254, 661, 796, 1073
MfeI CAATTG 1 cut(s) 982
MhlI GDGCHC 2 cut(s) 324, 691
MluCI AATT 5 cut(s) 460, 551, 592, 982, 1013
MlyI GAGTC 4 cut(s) 803, 913, 987, 1022
MmeI TCCRAC 5 cut(s) 218, 637, 887, 1029, 1056
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 1 cut(s) 996
MslI CAYNNNNRTG 1 cut(s) 1023
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 1 cut(s) 481
MspR9I CCNGG 3 cut(s) 31, 57, 482
MunI CAATTG 1 cut(s) 982
Mva1269I GAATGC 1 cut(s) 561
MvaI CCWGG 2 cut(s) 31, 57
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 4 cut(s) 99, 372, 703, 749
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 1 cut(s) 838
NdeII GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
NlaIII CATG 9 cut(s) 179, 298, 730, 760, 842, 904, 1022, 1028, 1070
NlaIV GGNNCC 1 cut(s) 234
NmeAIII GCCGAG 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 678
NspI RCATGY 1 cut(s) 1070
NspV TTCGAA 2 cut(s) 50, 388
PciI ACATGT 1 cut(s) 1066
PctI GAATGC 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 3 cut(s) 276, 745, 862
PleI GAGTC 4 cut(s) 802, 912, 986, 1022
PpsI GAGTC 4 cut(s) 802, 912, 986, 1022
PscI ACATGT 1 cut(s) 1066
Psp1406I AACGTT 1 cut(s) 506
Psp6I CCWGG 2 cut(s) 29, 55
PspGI CCWGG 2 cut(s) 29, 55
PspN4I GGNNCC 1 cut(s) 234
PsrI GAACNNNNNNTAC 2 cut(s) 107, 139
RsaI GTAC 4 cut(s) 125, 186, 416, 1008
RsaNI GTAC 4 cut(s) 124, 185, 415, 1007
RseI CAYNNNNRTG 1 cut(s) 1023
SacII CCGCGG 1 cut(s) 275
SaqAI TTAA 1 cut(s) 996
SatI GCNGC 3 cut(s) 275, 744, 861
Sau3AI GATC 6 cut(s) 73, 179, 268, 882, 1089, 1097
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 4 cut(s) 803, 913, 987, 1022
ScrFI CCNGG 3 cut(s) 31, 57, 482
SduI GDGCHC 2 cut(s) 324, 691
SexAI ACCWGGT 1 cut(s) 55
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 768
Sfr303I CCGCGG 1 cut(s) 275
SfuI TTCGAA 2 cut(s) 50, 388
SgrBI CCGCGG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 1023
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SpeI ACTAGT 1 cut(s) 736
Sse9I AATT 5 cut(s) 460, 551, 592, 982, 1013
SsiI CCGC 7 cut(s) 81, 272, 274, 418, 634, 860, 943
SspI AATATT 2 cut(s) 993, 1125
SspMI CTAG 4 cut(s) 155, 210, 707, 737
StyD4I CCNGG 3 cut(s) 29, 55, 480
StyI CCWWGG 1 cut(s) 838
TaaI ACNGT 1 cut(s) 686
TaiI ACGT 3 cut(s) 321, 509, 517
TaqI TCGA 8 cut(s) 50, 76, 267, 388, 533, 818, 825, 894
TasI AATT 5 cut(s) 460, 551, 592, 982, 1013
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 277, 863
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 996
Tru9I TTAA 1 cut(s) 996
TscAI CASTG 3 cut(s) 340, 472, 691
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 1 cut(s) 743
Tsp45I GTSAC 1 cut(s) 678
TspDTI ATGAA 5 cut(s) 164, 260, 452, 710, 1007
TspGWI ACGGA 1 cut(s) 545
TspRI CASTG 3 cut(s) 340, 472, 691
VneI GTGCAC 2 cut(s) 320, 687
XapI RAATTY 1 cut(s) 1013
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 1070
XcmI CCANNNNNNNNNTGG 2 cut(s) 836, 907
XmiI GTMKAC 1 cut(s) 1071
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 4 cut(s) 155, 210, 707, 737
ZraI GACGTC 1 cut(s) 515
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.