Rh6DG435300

UPF0496 protein At1g20180-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011075
Physical Location & Seq
Forward (+)
61380947 .. 61383200
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG435300.1

Sequence Viewer

Length: 1137 bp
ATGAGGAAAAGTATGCGCTCCAGGTTGAGATCTATGTTTTCGAAACCAGGCAGCAGATCAAGGAAAGTCAATGACCGAGGCGGTGGAGGTAGCTTTTCCAGCAAGCTGAGTGTGAACGAGGAGTACTTGGAAGTTTTCAGAACAAAATCGTATCTAGAAATGTGGGATAGAGTTCATGGATCGGTACAGCGTCCAACGACAAATACTAGACTATCCTCTTCTCCATCGGTTCCTTTCTATATGAATCTCTCCGAAAATCTTCTCGATCCGCGGCAAGAAACCCTCAATGACATGATTAGTGAGGAGGGAATAAACGTGCACCACCTTCTCACTGACTACTTTGAAGCAAACTTAGAGGCTTGCCATCTATGCGAGTTACTCCTTCGAAGCATCCATCAAACGCACGCAGAGTACCGCAACATCAAGAAGGTGATGAAAAAGCTAAGCCAAAGGAACAATTACACTGACGAGCAATGCCGGGCTATTTTCCGAGAGCTTACAACGTTTGCACGTCTGAAAAACTCGTTGTCGATTATCAGCCCCCTGCAATTCCGTGACATTCACGATAGCTACTCGGCATTGCTCAATAATTTGACATCGAGACAGAAAAAGATTAGACGCAGGGCAAAGCGGAACAGGATTTTGAAGAAAGTTGGAGGAGTTGGTCTCGTAGTGTCACACAGTGCACTTTTGCTCGCTTTGCTAGTCTTTGCATTTCATAGCATGATTGGACTAGTGGCAGCGCCAGCAATCATGTCTTGTTCTATAGGTTTGTGCAAGAAGAAAATGGAGTCAGGAGAGGAGTGGCTCAAGTCGAGTTTCGACCACGAAATCCATGGGGAGCAACTTGATGTTGCGGCCAAAGGGATTTACATACTGATCAACGATTTCGATACCATGAGTCGGATGGTCAGGCGATTACAGGACGAGGTAGAACACCGCAAAGCTGTTGCAGATTTGTGTGTTAGGAATGGAGTCAATTGCGAAATATTTAATGAGGTTGTGCGAGAATTTCATGTCCATGACTCTAACTTTATGGAGCAGTTGGAGGAGCTTGAAGAACATGTATACTTGTGTATGCTGACGATCAACCGATCCAGAAGGCTTGTGCTTCAAGAAATATTCGCAACACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

378

Amino Acids

43.71

Weight (kDa)

9.2

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 62 - 374 9.7e-17 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1068
AccII CGCG 1 cut(s) 271
AciI CCGC 7 cut(s) 81, 269, 271, 415, 631, 857, 940
AclI AACGTT 1 cut(s) 503
AclWI GGATC 3 cut(s) 187, 260, 1089
AcoI YGGCCR 1 cut(s) 858
AcsI RAATTY 1 cut(s) 1010
AdeI CACNNNGTG 1 cut(s) 683
AfaI GTAC 3 cut(s) 125, 186, 413
AfiI CCNNNNNNNGG 1 cut(s) 903
AflIII ACRYGT 1 cut(s) 1063
AgsI TTSAA 4 cut(s) 344, 646, 1058, 1115
AhlI ACTAGT 1 cut(s) 733
AjiI CACGTC 1 cut(s) 512
AjnI CCWGG 2 cut(s) 20, 46
AluBI AGCT 7 cut(s) 93, 106, 442, 496, 570, 947, 1054
AluI AGCT 7 cut(s) 93, 106, 442, 496, 570, 947, 1054
Alw21I GWGCWC 2 cut(s) 321, 688
Alw26I GTCTC 2 cut(s) 595, 671
Alw44I GTGCAC 2 cut(s) 317, 684
AlwI GGATC 3 cut(s) 187, 260, 1089
AoxI GGCC 1 cut(s) 858
ApaLI GTGCAC 2 cut(s) 317, 684
ApeKI GCWGC 2 cut(s) 51, 740
ApoI RAATTY 1 cut(s) 1010
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 2 cut(s) 18, 745
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 442
AsuII TTCGAA 2 cut(s) 41, 385
BaeGI GKGCMC 2 cut(s) 321, 688
Bbv12I GWGCWC 2 cut(s) 321, 688
BbvI GCAGC 2 cut(s) 63, 752
BccI CCATC 4 cut(s) 232, 372, 402, 901
BciT130I CCWGG 2 cut(s) 22, 48
BclI TGATCA 1 cut(s) 879
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 595, 671
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 4 cut(s) 155, 207, 704, 734
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 746
BglII AGATCT 1 cut(s) 29
BisI GCNGC 4 cut(s) 52, 272, 741, 858
BlpI GCTNAGC 1 cut(s) 443
BlsI GCNGC 4 cut(s) 53, 273, 742, 859
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 3 cut(s) 22, 48, 479
BmgBI CACGTC 1 cut(s) 512
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 3 cut(s) 22, 48, 479
BmsI GCATC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 651, 683
BpmI CTGGAG 1 cut(s) 4
Bpu1102I GCTNAGC 1 cut(s) 443
Bpu14I TTCGAA 2 cut(s) 41, 385
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 479
BsaI GGTCTC 1 cut(s) 671
BsaJI CCNNGG 3 cut(s) 76, 269, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 903
Bse3DI GCAATG 2 cut(s) 479, 578
BseBI CCWGG 2 cut(s) 22, 48
BseDI CCNNGG 3 cut(s) 76, 269, 835
BseGI GGATG 2 cut(s) 390, 912
BseLI CCNNNNNNNGG 1 cut(s) 903
BseMI GCAATG 2 cut(s) 479, 578
BseMII CTCAG 1 cut(s) 98
BseRI GAGGAG 5 cut(s) 134, 317, 672, 815, 1064
BseSI GKGCMC 2 cut(s) 321, 688
BseXI GCAGC 2 cut(s) 63, 752
Bsh1236I CGCG 1 cut(s) 271
BshFI GGCC 1 cut(s) 860
BsiHKAI GWGCWC 2 cut(s) 321, 688
BsiSI CCGG 1 cut(s) 478
BslI CCNNNNNNNGG 1 cut(s) 903
BsmAI GTCTC 2 cut(s) 595, 671
BsnI GGCC 1 cut(s) 860
Bso31I GGTCTC 1 cut(s) 671
Bsp119I TTCGAA 2 cut(s) 41, 385
Bsp1286I GDGCHC 2 cut(s) 321, 688
Bsp143I GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
Bsp1720I GCTNAGC 1 cut(s) 443
Bsp19I CCATGG 1 cut(s) 835
BspACI CCGC 7 cut(s) 81, 269, 271, 415, 631, 857, 940
BspANI GGCC 1 cut(s) 860
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 3 cut(s) 187, 260, 1089
BspT104I TTCGAA 2 cut(s) 41, 385
BspTNI GGTCTC 1 cut(s) 671
BsrDI GCAATG 2 cut(s) 479, 578
BssECI CCNNGG 3 cut(s) 76, 269, 835
BssMI GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
BssNAI GTATAC 1 cut(s) 1069
BssT1I CCWWGG 1 cut(s) 835
Bst1107I GTATAC 1 cut(s) 1069
Bst2UI CCWGG 2 cut(s) 22, 48
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 1 cut(s) 223
BstBI TTCGAA 2 cut(s) 41, 385
BstC8I GCNNGC 5 cut(s) 104, 361, 405, 696, 747
BstDEI CTNAG 3 cut(s) 107, 352, 443
BstDSI CCRYGG 2 cut(s) 269, 835
BstF5I GGATG 2 cut(s) 390, 912
BstFNI CGCG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 746
BstHHI GCGC 2 cut(s) 18, 745
BstKTI GATC 7 cut(s) 32, 59, 182, 268, 882, 1089, 1097
BstMAI GTCTC 2 cut(s) 595, 671
BstMBI GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
BstMWI GCNNNNNNNGC 4 cut(s) 99, 369, 700, 746
BstNI CCWGG 2 cut(s) 22, 48
BstNSI RCATGY 1 cut(s) 1067
BstSCI CCNGG 3 cut(s) 20, 46, 477
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 2 cut(s) 321, 688
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 63, 752
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BstZ17I GTATAC 1 cut(s) 1069
BsuRI GGCC 1 cut(s) 860
BtgI CCRYGG 2 cut(s) 269, 835
BtrI CACGTC 1 cut(s) 512
BtsCI GGATG 2 cut(s) 390, 912
BtsIMutI CAGTG 3 cut(s) 330, 462, 688
Cac8I GCNNGC 5 cut(s) 104, 361, 405, 696, 747
CfoI GCGC 2 cut(s) 18, 745
Cfr42I CCGCGG 1 cut(s) 272
CseI GACGC 2 cut(s) 179, 627
Csp6I GTAC 3 cut(s) 124, 185, 412
CviAII CATG 9 cut(s) 176, 292, 724, 754, 836, 898, 1016, 1022, 1064
CviQI GTAC 3 cut(s) 124, 185, 412
DdeI CTNAG 3 cut(s) 107, 352, 443
DpnI GATC 7 cut(s) 31, 58, 181, 267, 881, 1088, 1096
DpnII GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
DraIII CACNNNGTG 1 cut(s) 683
EaeI YGGCCR 1 cut(s) 858
Eam1104I CTCTTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 835
Eco31I GGTCTC 1 cut(s) 671
EcoRII CCWGG 2 cut(s) 20, 46
EcoT14I CCWWGG 1 cut(s) 835
ErhI CCWWGG 1 cut(s) 835
FaeI CATG 9 cut(s) 179, 295, 727, 757, 839, 901, 1019, 1025, 1067
FatI CATG 9 cut(s) 175, 291, 723, 753, 835, 897, 1015, 1021, 1063
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 1 cut(s) 1068
Fnu4HI GCNGC 4 cut(s) 52, 272, 741, 858
FokI GGATG 2 cut(s) 377, 919
Fsp4HI GCNGC 4 cut(s) 52, 272, 741, 858
FspBI CTAG 4 cut(s) 155, 207, 704, 734
GlaI GCGC 2 cut(s) 17, 744
GluI GCNGC 4 cut(s) 52, 272, 741, 858
GsuI CTGGAG 1 cut(s) 4
HaeII RGCGCY 1 cut(s) 746
HaeIII GGCC 1 cut(s) 860
HapII CCGG 1 cut(s) 478
HgaI GACGC 2 cut(s) 179, 627
HhaI GCGC 2 cut(s) 18, 745
Hin1II CATG 9 cut(s) 179, 295, 727, 757, 839, 901, 1019, 1025, 1067
Hin6I GCGC 2 cut(s) 16, 743
HinP1I GCGC 2 cut(s) 16, 743
HinfI GANTC 5 cut(s) 244, 791, 901, 975, 1025
HpaII CCGG 1 cut(s) 478
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 4 cut(s) 115, 319, 686, 1069
Hpy188I TCNGA 5 cut(s) 140, 253, 491, 516, 906
Hpy188III TCNNGA 8 cut(s) 155, 263, 424, 563, 600, 795, 1098, 1115
Hpy8I GTNNAC 4 cut(s) 115, 319, 686, 1069
HpyAV CCTTC 4 cut(s) 335, 392, 421, 1095
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 3 cut(s) 315, 503, 511
HpyCH4V TGCA 7 cut(s) 319, 509, 547, 686, 713, 777, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 369, 700, 746
HpyF3I CTNAG 3 cut(s) 107, 352, 443
HpySE526I ACGT 3 cut(s) 315, 503, 511
Hsp92II CATG 9 cut(s) 179, 295, 727, 757, 839, 901, 1019, 1025, 1067
HspAI GCGC 2 cut(s) 16, 743
Ksp22I TGATCA 1 cut(s) 879
KspI CCGCGG 1 cut(s) 272
Kzo9I GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
LmnI GCTCC 4 cut(s) 23, 841, 1039, 1051
Lsp1109I GCAGC 2 cut(s) 63, 752
LweI GCATC 1 cut(s) 399
MaeI CTAG 4 cut(s) 155, 207, 704, 734
MaeII ACGT 3 cut(s) 315, 503, 511
MaeIII GTNAC 3 cut(s) 375, 554, 675
MalI GATC 7 cut(s) 31, 58, 181, 267, 881, 1088, 1096
MboI GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
MboII GAAGA 5 cut(s) 210, 251, 658, 793, 1070
MfeI CAATTG 1 cut(s) 979
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 321, 688
MluCI AATT 5 cut(s) 457, 548, 589, 979, 1010
MlyI GAGTC 4 cut(s) 800, 910, 984, 1019
MmeI TCCRAC 4 cut(s) 218, 634, 884, 1026
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 1 cut(s) 993
MslI CAYNNNNRTG 1 cut(s) 1020
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 1 cut(s) 478
MspR9I CCNGG 3 cut(s) 22, 48, 479
MunI CAATTG 1 cut(s) 979
MvaI CCWGG 2 cut(s) 22, 48
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 4 cut(s) 99, 369, 700, 746
NciI CCSGG 1 cut(s) 479
NcoI CCATGG 1 cut(s) 835
NdeII GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
NlaIII CATG 9 cut(s) 179, 295, 727, 757, 839, 901, 1019, 1025, 1067
NlaIV GGNNCC 1 cut(s) 231
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 2 cut(s) 554, 675
NspI RCATGY 1 cut(s) 1067
NspV TTCGAA 2 cut(s) 41, 385
PciI ACATGT 1 cut(s) 1063
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 4 cut(s) 53, 273, 742, 859
PleI GAGTC 4 cut(s) 799, 909, 983, 1019
PpsI GAGTC 4 cut(s) 799, 909, 983, 1019
PscI ACATGT 1 cut(s) 1063
Psp1406I AACGTT 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 20, 46
PspGI CCWGG 2 cut(s) 20, 46
PspN4I GGNNCC 1 cut(s) 231
PsrI GAACNNNNNNTAC 2 cut(s) 107, 139
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 3 cut(s) 125, 186, 413
RsaNI GTAC 3 cut(s) 124, 185, 412
RseI CAYNNNNRTG 1 cut(s) 1020
SacII CCGCGG 1 cut(s) 272
SaqAI TTAA 1 cut(s) 993
SatI GCNGC 4 cut(s) 52, 272, 741, 858
Sau3AI GATC 7 cut(s) 29, 56, 179, 265, 879, 1086, 1094
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 4 cut(s) 800, 910, 984, 1019
ScrFI CCNGG 3 cut(s) 22, 48, 479
SduI GDGCHC 2 cut(s) 321, 688
SfaNI GCATC 1 cut(s) 399
SfcI CTRYAG 1 cut(s) 765
Sfr303I CCGCGG 1 cut(s) 272
SfuI TTCGAA 2 cut(s) 41, 385
SgrBI CCGCGG 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 1020
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
SpeI ACTAGT 1 cut(s) 733
Sse9I AATT 5 cut(s) 457, 548, 589, 979, 1010
SsiI CCGC 7 cut(s) 81, 269, 271, 415, 631, 857, 940
SspI AATATT 2 cut(s) 990, 1122
SspMI CTAG 4 cut(s) 155, 207, 704, 734
StyD4I CCNGG 3 cut(s) 20, 46, 477
StyI CCWWGG 1 cut(s) 835
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 3 cut(s) 318, 506, 514
TaqI TCGA 8 cut(s) 41, 264, 385, 530, 599, 815, 822, 891
TaqII GACCGA 1 cut(s) 90
TasI AATT 5 cut(s) 457, 548, 589, 979, 1010
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 274, 860
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 993
Tru9I TTAA 1 cut(s) 993
TscAI CASTG 3 cut(s) 337, 469, 688
TseFI GTSAC 2 cut(s) 554, 675
TseI GCWGC 2 cut(s) 51, 740
Tsp45I GTSAC 2 cut(s) 554, 675
TspDTI ATGAA 5 cut(s) 164, 257, 449, 707, 1004
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 3 cut(s) 337, 469, 688
VneI GTGCAC 2 cut(s) 317, 684
XapI RAATTY 1 cut(s) 1010
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 1067
XcmI CCANNNNNNNNNTGG 2 cut(s) 833, 904
XmiI GTMKAC 1 cut(s) 1068
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 4 cut(s) 155, 207, 704, 734
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.