FvH4_2g38050

UPF0496 protein At1g20180-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011075
Physical Location & Seq
Reverse (-)
27575508 .. 27577257
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38050.t1

Sequence Viewer

Length: 1143 bp
ATGAGGAAGAGTCTACGATCCGGGTTGAGATCTATGTTTTCGAAACCAGGCAGCAGATCAAGGAAAATTGATCACCGAGGCGGTGGAGGTAGCTTTTCCAGTAAGCTAAGTGTGAACGACGAGTATTTGGAAGTTTTCAGAACAAAATCATATCTAGAAATGTGGGATAGAGTTCATGGATCGGTACAATATCCAACAACAAACCCTAATCTACTATCTTCTTCTCCATCAGTTCCTTTCTATGTGAATCTATCAGAAAATCTGCTCGATCCGCGGCAAGAAACCCTCAATGAGATGATTAGCGAGGATGGAATAAACGTGCACCACCTTCTCACCGACTACTTTGAAGCGAGCCTAGAGGCGTGCCATATATGCGAGCTACTCCTCAGAAGCATCCATCAAACGCACGCAGAGTACGGCAACATAAAGAAAGTGATGAAGAAGCTAAGCCAAAGAAGCATTTACACCGACGAGCAATGCCGGGCTATTTTCCGGGAGCTTACAACGTTTGCACGTCTCAAAAACCCGTTGTCGATTATCAGCCCCCTGCAATTCCGTGACATTCACGATAGCTACTCAGCATTGCTCAGTAGCCTGACATCGAGACAGCAGAAGATTCGGCGTAGGGCAAAGCGTAACAGGATTCTGAAGAAAGTTGGAGGAGTTGGACTGGTGGTGTCACACAGTGCACTTTTGATAGGTTTGCTAGTCTTTGCATTTCATAGCATGATCGGAATAGTGGCAGCGCCGGCAATCATGGCTTGTTCTGCAGGTTTGTGCAAGAAGAAAATGGAGTCAGGAGAGCAGTGGTTCAAGTCAAGATTCGACGTCCACGAAATTCACGGGGAGCAACTCGATGTTGCAGCCAAAGGGATTTACATACTGATCAACGATTTCGATACCATGAGTCGGCTGGTCATGAGATTACAGGATGAGGTAGAACACCGCAAAGCTGTTGCAGATTTGTGTGTGAGGAATGGAATTAACTGTGAAGTATTCAAGGGGGTTGTGCGAGAATTTCATGTCCATGACTCTAACTTCATGGAGCAGTTGGAGGAGCTTGAAGAACATGTATACTTGTGTATGCTGACGATCAACAAATCAAGAAGGCTTGTGCTTCAAGAAATATTGGCAACACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

381

Amino Acids

43.42

Weight (kDa)

9.01

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 106 - 376 4.8e-17 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20180 AT1G20180
fragaria_vesca FvH4_2g38050
malus_domestica MD03G1275300.v1.1 MD08G1009100.v1.1 MD15G1007800.v1.1 MD15G1007900.v1.1
prunus_persica Prupe.1G360800_v2.0.a1 Prupe.1G360800_v2.0.a1
pyrus_communis pycom15g00630
rosa_chinensis RchiOBHm_Chr6g0303541
rosa_laevigata RLG00000011075
rosa_multiflora Rmu_ssc0000114.1_g000034
rosa_roxburghii Rroxscaffold_7G00164660
rosa_rugosa Rorug06G0322100
rosa_samantha Rh6AG436500 Rh6BG466900 Rh6CG448500 Rh6DG435300
rosa_wichuraiana Rw6G037780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 831
Acc36I ACCTGC 1 cut(s) 761
AccI GTMKAC 2 cut(s) 13, 1074
AccII CGCG 1 cut(s) 274
AciI CCGC 4 cut(s) 81, 272, 274, 946
AclI AACGTT 1 cut(s) 506
AclWI GGATC 3 cut(s) 12, 187, 263
AcsI RAATTY 2 cut(s) 837, 1016
AcuI CTGAAG 1 cut(s) 668
AcyI GRCGYC 1 cut(s) 828
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 2 cut(s) 186, 416
AfiI CCNNNNNNNGG 1 cut(s) 909
AflIII ACRYGT 1 cut(s) 1069
AgsI TTSAA 5 cut(s) 347, 814, 1000, 1064, 1121
AjiI CACGTC 1 cut(s) 515
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 8 cut(s) 93, 106, 379, 445, 499, 573, 953, 1060
AluI AGCT 8 cut(s) 93, 106, 379, 445, 499, 573, 953, 1060
Alw21I GWGCWC 2 cut(s) 324, 691
Alw26I GTCTC 2 cut(s) 521, 598
Alw44I GTGCAC 2 cut(s) 320, 687
AlwI GGATC 3 cut(s) 12, 187, 263
ApaLI GTGCAC 2 cut(s) 320, 687
ApeKI GCWGC 3 cut(s) 51, 743, 863
ApoI RAATTY 2 cut(s) 837, 1016
AspLEI GCGC 1 cut(s) 748
AsuC2I CCSGG 3 cut(s) 22, 482, 494
AsuHPI GGTGA 2 cut(s) 65, 325
AsuII TTCGAA 1 cut(s) 41
BaeGI GKGCMC 2 cut(s) 324, 691
Bbv12I GWGCWC 2 cut(s) 324, 691
BbvI GCAGC 3 cut(s) 63, 755, 875
BccI CCATC 3 cut(s) 235, 302, 405
BceAI ACGGC 1 cut(s) 433
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 1 cut(s) 48
BclI TGATCA 2 cut(s) 70, 885
BcnI CCSGG 3 cut(s) 22, 482, 494
BcoDI GTCTC 2 cut(s) 521, 598
BfaI CTAG 3 cut(s) 155, 356, 707
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 761
BglII AGATCT 1 cut(s) 29
BisI GCNGC 4 cut(s) 52, 275, 744, 864
BlpI GCTNAGC 1 cut(s) 446
BlsI GCNGC 4 cut(s) 53, 276, 745, 865
Bme1390I CCNGG 4 cut(s) 22, 48, 482, 494
BmgBI CACGTC 1 cut(s) 515
BmrFI CCNGG 4 cut(s) 22, 48, 482, 494
BmsI GCATC 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 446
Bpu14I TTCGAA 1 cut(s) 41
BpuMI CCSGG 3 cut(s) 22, 482, 494
BsaHI GRCGYC 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 76, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 909
Bse118I RCCGGY 1 cut(s) 748
Bse1I ACTGG 2 cut(s) 99, 675
Bse3DI GCAATG 2 cut(s) 482, 581
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 2 cut(s) 76, 272
BseGI GGATG 3 cut(s) 313, 393, 937
BseLI CCNNNNNNNGG 1 cut(s) 909
BseMI GCAATG 2 cut(s) 482, 581
BseMII CTCAG 3 cut(s) 400, 591, 601
BseNI ACTGG 2 cut(s) 99, 675
BseRI GAGGAG 3 cut(s) 374, 675, 1070
BseSI GKGCMC 2 cut(s) 324, 691
BseXI GCAGC 3 cut(s) 63, 755, 875
Bsh1236I CGCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 324, 691
BsiSI CCGG 4 cut(s) 21, 481, 493, 749
BslI CCNNNNNNNGG 1 cut(s) 909
BsmAI GTCTC 2 cut(s) 521, 598
BsmBI CGTCTC 1 cut(s) 521
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 324, 691
Bsp143I GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
Bsp1720I GCTNAGC 1 cut(s) 446
BspACI CCGC 4 cut(s) 81, 272, 274, 946
BspCNI CTCAG 3 cut(s) 399, 590, 600
BspFNI CGCG 1 cut(s) 274
BspHI TCATGA 1 cut(s) 918
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 1 cut(s) 761
BspPI GGATC 3 cut(s) 12, 187, 263
BspT104I TTCGAA 1 cut(s) 41
BsrDI GCAATG 2 cut(s) 482, 581
BsrFI RCCGGY 1 cut(s) 748
BsrI ACTGG 2 cut(s) 99, 675
BssAI RCCGGY 1 cut(s) 748
BssECI CCNNGG 2 cut(s) 76, 272
BssMI GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
BssNAI GTATAC 1 cut(s) 1075
BssNI GRCGYC 1 cut(s) 828
Bst1107I GTATAC 1 cut(s) 1075
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 686, 989
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 1 cut(s) 828
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 5 cut(s) 352, 364, 377, 408, 750
BstDEI CTNAG 5 cut(s) 107, 386, 446, 577, 587
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 3 cut(s) 313, 393, 937
BstFNI CGCG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 749
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 9 cut(s) 20, 32, 59, 73, 182, 271, 732, 888, 1095
BstMAI GTCTC 2 cut(s) 521, 598
BstMBI GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
BstMWI GCNNNNNNNGC 6 cut(s) 271, 372, 456, 749, 758, 767
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 1073
BstSCI CCNGG 4 cut(s) 20, 46, 480, 492
BstSFI CTRYAG 1 cut(s) 768
BstSLI GKGCMC 2 cut(s) 324, 691
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 3 cut(s) 63, 755, 875
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BstZ17I GTATAC 1 cut(s) 1075
BtgI CCRYGG 1 cut(s) 272
BtrI CACGTC 1 cut(s) 515
BtsCI GGATG 3 cut(s) 313, 393, 937
BtsI GCAGTG 1 cut(s) 812
BtsIMutI CAGTG 2 cut(s) 691, 812
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 5 cut(s) 352, 364, 377, 408, 750
CciI TCATGA 1 cut(s) 918
CfoI GCGC 1 cut(s) 748
Cfr10I RCCGGY 1 cut(s) 748
Cfr42I CCGCGG 1 cut(s) 275
Csp6I GTAC 2 cut(s) 185, 415
CviAII CATG 9 cut(s) 176, 727, 757, 904, 919, 1022, 1028, 1042, 1070
CviQI GTAC 2 cut(s) 185, 415
DdeI CTNAG 5 cut(s) 107, 386, 446, 577, 587
DpnI GATC 9 cut(s) 19, 31, 58, 72, 181, 270, 731, 887, 1094
DpnII GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
DraIII CACNNNGTG 1 cut(s) 686
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco57I CTGAAG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 46
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 9 cut(s) 179, 730, 760, 907, 922, 1025, 1031, 1045, 1073
FatI CATG 9 cut(s) 175, 726, 756, 903, 918, 1021, 1027, 1041, 1069
FbaI TGATCA 2 cut(s) 70, 885
FblI GTMKAC 2 cut(s) 13, 1074
Fnu4HI GCNGC 4 cut(s) 52, 275, 744, 864
FokI GGATG 3 cut(s) 320, 380, 944
Fsp4HI GCNGC 4 cut(s) 52, 275, 744, 864
FspBI CTAG 3 cut(s) 155, 356, 707
GlaI GCGC 1 cut(s) 747
GluI GCNGC 4 cut(s) 52, 275, 744, 864
HaeII RGCGCY 1 cut(s) 749
HapII CCGG 4 cut(s) 21, 481, 493, 749
HhaI GCGC 1 cut(s) 748
Hin1I GRCGYC 1 cut(s) 828
Hin1II CATG 9 cut(s) 179, 730, 760, 907, 922, 1025, 1031, 1045, 1073
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HinfI GANTC 8 cut(s) 10, 247, 616, 643, 794, 822, 907, 1031
HpaII CCGG 4 cut(s) 21, 481, 493, 749
HphI GGTGA 2 cut(s) 65, 325
Hpy166II GTNNAC 6 cut(s) 14, 115, 322, 689, 832, 1075
Hpy188I TCNGA 5 cut(s) 140, 256, 389, 648, 734
Hpy188III TCNNGA 8 cut(s) 155, 566, 603, 798, 819, 919, 1104, 1121
Hpy8I GTNNAC 6 cut(s) 14, 115, 322, 689, 832, 1075
Hpy99I CGWCG 3 cut(s) 122, 473, 830
HpyAV CCTTC 2 cut(s) 338, 1101
HpyCH4III ACNGT 2 cut(s) 686, 989
HpyCH4IV ACGT 4 cut(s) 318, 506, 514, 828
HpyCH4V TGCA 9 cut(s) 322, 512, 550, 689, 716, 770, 780, 863, 959
HpyF10VI GCNNNNNNNGC 6 cut(s) 271, 372, 456, 749, 758, 767
HpyF3I CTNAG 5 cut(s) 107, 386, 446, 577, 587
HpySE526I ACGT 4 cut(s) 318, 506, 514, 828
Hsp92I GRCGYC 1 cut(s) 828
Hsp92II CATG 9 cut(s) 179, 730, 760, 907, 922, 1025, 1031, 1045, 1073
HspAI GCGC 1 cut(s) 746
KroI GCCGGC 1 cut(s) 748
KroNI GCCGGC 1 cut(s) 750
Ksp22I TGATCA 2 cut(s) 70, 885
KspI CCGCGG 1 cut(s) 275
Kzo9I GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
LmnI GCTCC 4 cut(s) 496, 847, 1045, 1057
Lsp1109I GCAGC 3 cut(s) 63, 755, 875
LweI GCATC 1 cut(s) 402
MaeI CTAG 3 cut(s) 155, 356, 707
MaeII ACGT 4 cut(s) 318, 506, 514, 828
MaeIII GTNAC 3 cut(s) 557, 635, 678
MalI GATC 9 cut(s) 19, 31, 58, 72, 181, 270, 731, 887, 1094
MboI GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
MboII GAAGA 8 cut(s) 19, 210, 213, 451, 625, 661, 796, 1076
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 324, 691
MluCI AATT 5 cut(s) 66, 551, 837, 981, 1016
MlyI GAGTC 4 cut(s) 19, 803, 916, 1025
MmeI TCCRAC 4 cut(s) 218, 637, 646, 1032
MroNI GCCGGC 1 cut(s) 748
MseI TTAA 1 cut(s) 984
MslI CAYNNNNRTG 1 cut(s) 1026
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 4 cut(s) 21, 481, 493, 749
MspR9I CCNGG 4 cut(s) 22, 48, 482, 494
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 6 cut(s) 271, 372, 456, 749, 758, 767
NaeI GCCGGC 1 cut(s) 750
NciI CCSGG 3 cut(s) 22, 482, 494
NdeII GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
NgoMIV GCCGGC 1 cut(s) 748
NlaIII CATG 9 cut(s) 179, 730, 760, 907, 922, 1025, 1031, 1045, 1073
NmuCI GTSAC 2 cut(s) 557, 678
NspI RCATGY 1 cut(s) 1073
NspV TTCGAA 1 cut(s) 41
PagI TCATGA 1 cut(s) 918
PciI ACATGT 1 cut(s) 1069
PcsI WCGNNNNNNNCGW 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 750
PfeI GAWTC 4 cut(s) 247, 616, 643, 822
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 4 cut(s) 53, 276, 745, 865
PleI GAGTC 4 cut(s) 18, 802, 915, 1025
PpsI GAGTC 4 cut(s) 18, 802, 915, 1025
PscI ACATGT 1 cut(s) 1069
Psp1406I AACGTT 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 2 cut(s) 186, 416
RsaNI GTAC 2 cut(s) 185, 415
RseI CAYNNNNRTG 1 cut(s) 1026
SacII CCGCGG 1 cut(s) 275
SaqAI TTAA 1 cut(s) 984
SatI GCNGC 4 cut(s) 52, 275, 744, 864
Sau3AI GATC 9 cut(s) 17, 29, 56, 70, 179, 268, 729, 885, 1092
SchI GAGTC 4 cut(s) 19, 803, 916, 1025
ScrFI CCNGG 4 cut(s) 22, 48, 482, 494
SduI GDGCHC 2 cut(s) 324, 691
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 768
Sfr303I CCGCGG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 41
SgrBI CCGCGG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 1026
Sse9I AATT 5 cut(s) 66, 551, 837, 981, 1016
SsiI CCGC 4 cut(s) 81, 272, 274, 946
SspI AATATT 1 cut(s) 1128
SspMI CTAG 3 cut(s) 155, 356, 707
StyD4I CCNGG 4 cut(s) 20, 46, 480, 492
TaaI ACNGT 2 cut(s) 686, 989
TaiI ACGT 4 cut(s) 321, 509, 517, 831
TaqI TCGA 7 cut(s) 41, 267, 533, 602, 825, 855, 897
TasI AATT 5 cut(s) 66, 551, 837, 981, 1016
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 4 cut(s) 247, 616, 643, 822
Tru1I TTAA 1 cut(s) 984
Tru9I TTAA 1 cut(s) 984
TscAI CASTG 2 cut(s) 691, 812
TseFI GTSAC 2 cut(s) 557, 678
TseI GCWGC 3 cut(s) 51, 743, 863
Tsp45I GTSAC 2 cut(s) 557, 678
TspDTI ATGAA 5 cut(s) 164, 452, 710, 1010, 1030
TspGWI ACGGA 1 cut(s) 545
TspRI CASTG 2 cut(s) 691, 812
VneI GTGCAC 2 cut(s) 320, 687
XapI RAATTY 2 cut(s) 837, 1016
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 1073
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
XmiI GTMKAC 2 cut(s) 13, 1074
XspI CTAG 3 cut(s) 155, 356, 707
ZraI GACGTC 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.