AT1G20225

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009565
Physical Location & Seq
Reverse (-)
7007756 .. 7009380
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20225.1

Sequence Viewer

Length: 702 bp
ATGATGATTCGAACGGCGTTAGTGTTCGTTGTCTTCTTCGTCGGAACAGTCGTACAAGCGCAGCTGATACCTCCGGCGAGGCGTGATGGATTTTTGTATCCACCGGGTCGGAAAATCGACCGGGATACGATACTTATCGAGGCGTATATTGACCCGGTTTGTCCGGATTGTAGAGACGCTTGGGAGCCGCTAAAGCTAGCGATTGATCACTATGGATCTCGCGTTGCTCTTGTTCTTCACCTCATTCCTTTACCCTTCCATGACAATGCATTTGTAGCATCTCGGGCTTTACACATCGTTGATACCTTAAACGCCAATGCCACGTTCAATTTGCTGGAAGGGATCTTCAAGCATCAGACATTGTTCTACAACTCGCAAACACAACTCATGTCAAGACCTGCAGTTGTGGAAGAATTAATCAAGCTTGGAACAGTTACGTTAGGAAACTCATATCATTCTCCTTTGAAATCTGGCTTCAGCAACTCAAAATCTGATCTTGCCACTAGAGTTTCCTTCAAGTATAGCGTCTCAAGAGGAGTCTCCGCGACACCAACCTTCTACGTGAATGGATTCGAGCTGCCTGGTGCTGGTTCTCCCAAAGATTATGAAGGATGGAGAGACACCATTGATCCTCTGGTTAAACCACAAGAAGTAGACATACGAGACACCTTCCTTTCATTCCTCACGCAACCATCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin_4

Protein Analysis

233

Amino Acids

26.05

Weight (kDa)

6.59

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_4 PF13462 46 - 210 6.8e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 654
AccII CGCG 2 cut(s) 222, 545
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 2 cut(s) 188, 543
AclWI GGATC 3 cut(s) 223, 350, 623
AcuI CTGAAG 1 cut(s) 460
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 587
AgsI TTSAA 4 cut(s) 328, 349, 466, 517
AjnI CCWGG 1 cut(s) 580
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 4 cut(s) 64, 196, 424, 577
AluI AGCT 4 cut(s) 64, 196, 424, 577
Alw26I GTCTC 5 cut(s) 168, 532, 544, 612, 657
AlwI GGATC 3 cut(s) 223, 350, 623
Ama87I CYCGRG 1 cut(s) 282
Aor13HI TCCGGA 1 cut(s) 163
ApeKI GCWGC 2 cut(s) 61, 577
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AseI ATTAAT 1 cut(s) 416
Asp700I GAANNNNTTC 1 cut(s) 569
AspLEI GCGC 1 cut(s) 61
AsuC2I CCSGG 3 cut(s) 105, 122, 155
AsuHPI GGTGA 1 cut(s) 230
AsuII TTCGAA 1 cut(s) 10
AsuNHI GCTAGC 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 642, 674
BbsI GAAGAC 1 cut(s) 25
BbvI GCAGC 2 cut(s) 73, 564
BccI CCATC 3 cut(s) 80, 606, 700
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 582
BciVI GTATCC 2 cut(s) 108, 118
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 3 cut(s) 105, 122, 155
BcoDI GTCTC 5 cut(s) 168, 532, 544, 612, 657
BfaI CTAG 3 cut(s) 197, 504, 700
BfmI CTRYAG 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 406
BfuI GTATCC 2 cut(s) 108, 118
BisI GCNGC 3 cut(s) 62, 188, 578
BlsI GCNGC 3 cut(s) 63, 189, 579
Bme1390I CCNGG 4 cut(s) 105, 122, 155, 582
BmeT110I CYCGRG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 4 cut(s) 105, 122, 155, 582
BmsI GCATC 2 cut(s) 287, 361
BmtI GCTAGC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 514
BpuMI CCSGG 3 cut(s) 105, 122, 155
BsaAI YACGTR 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 134
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse8I GATNNNNATC 1 cut(s) 134
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 582
BseGI GGATG 1 cut(s) 617
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 1 cut(s) 587
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 2 cut(s) 73, 564
Bsh1236I CGCG 2 cut(s) 222, 545
Bsh1285I CGRYCG 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 5 cut(s) 74, 104, 121, 155, 164
BslI CCNNNNNNNGG 1 cut(s) 587
BsmAI GTCTC 5 cut(s) 168, 532, 544, 612, 657
BsmBI CGTCTC 2 cut(s) 168, 532
BsoBI CYCGRG 1 cut(s) 282
Bsp119I TTCGAA 1 cut(s) 10
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 205, 215, 342, 493, 628
BspACI CCGC 2 cut(s) 188, 543
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 2 cut(s) 222, 545
BspLI GGNNCC 1 cut(s) 186
BspMAI CTGCAG 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 406
BspOI GCTAGC 1 cut(s) 200
BspPI GGATC 3 cut(s) 223, 350, 623
BspT104I TTCGAA 1 cut(s) 10
BssMI GATC 5 cut(s) 205, 215, 342, 493, 628
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 49, 433
BstBAI YACGTR 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 198
BstF5I GGATG 1 cut(s) 617
BstFNI CGCG 2 cut(s) 222, 545
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 5 cut(s) 208, 218, 345, 496, 631
BstMAI GTCTC 5 cut(s) 168, 532, 544, 612, 657
BstMBI GATC 5 cut(s) 205, 215, 342, 493, 628
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 3 cut(s) 193, 275, 284
BstNI CCWGG 1 cut(s) 582
BstSCI CCNGG 4 cut(s) 103, 120, 153, 580
BstSFI CTRYAG 1 cut(s) 399
BstUI CGCG 2 cut(s) 222, 545
BstV1I GCAGC 2 cut(s) 73, 564
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 215, 342
BstYI RGATCY 2 cut(s) 215, 342
BsuI GTATCC 2 cut(s) 108, 118
BtsCI GGATG 1 cut(s) 617
BveI ACCTGC 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 1 cut(s) 61
CseI GACGC 2 cut(s) 185, 514
Csp6I GTAC 1 cut(s) 53
CviAII CATG 2 cut(s) 260, 388
CviJI RGCY 7 cut(s) 64, 187, 196, 287, 424, 474, 577
CviKI_1 RGCY 7 cut(s) 64, 187, 196, 287, 424, 474, 577
CviQI GTAC 1 cut(s) 53
DpnI GATC 5 cut(s) 207, 217, 344, 495, 630
DpnII GATC 5 cut(s) 205, 215, 342, 493, 628
Eco57I CTGAAG 1 cut(s) 460
Eco88I CYCGRG 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 580
EcoT22I ATGCAT 1 cut(s) 271
Esp3I CGTCTC 2 cut(s) 168, 532
FaeI CATG 2 cut(s) 263, 391
FaiI YATR 8 cut(s) 147, 213, 261, 389, 451, 522, 606, 659
FatI CATG 2 cut(s) 259, 387
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 3 cut(s) 62, 188, 578
FokI GGATG 1 cut(s) 624
Fsp4HI GCNGC 3 cut(s) 62, 188, 578
FspBI CTAG 3 cut(s) 197, 504, 700
GlaI GCGC 1 cut(s) 60
GluI GCNGC 3 cut(s) 62, 188, 578
HapII CCGG 5 cut(s) 74, 104, 121, 155, 164
HgaI GACGC 2 cut(s) 185, 514
HhaI GCGC 1 cut(s) 61
Hin1II CATG 2 cut(s) 263, 391
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 3 cut(s) 7, 537, 570
HpaII CCGG 5 cut(s) 74, 104, 121, 155, 164
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 655
Hpy188I TCNGA 4 cut(s) 44, 111, 357, 493
Hpy188III TCNNGA 3 cut(s) 164, 393, 531
Hpy8I GTNNAC 1 cut(s) 655
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 6 cut(s) 265, 332, 523, 565, 602, 679
HpyCH4III ACNGT 2 cut(s) 49, 433
HpyCH4IV ACGT 3 cut(s) 323, 437, 561
HpyCH4V TGCA 2 cut(s) 269, 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 275, 284
HpySE526I ACGT 3 cut(s) 323, 437, 561
Hsp92II CATG 2 cut(s) 263, 391
HspAI GCGC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 5 cut(s) 205, 215, 342, 493, 628
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 73, 564
LweI GCATC 2 cut(s) 287, 361
MaeI CTAG 3 cut(s) 197, 504, 700
MaeII ACGT 3 cut(s) 323, 437, 561
MaeIII GTNAC 1 cut(s) 433
MalI GATC 5 cut(s) 207, 217, 344, 495, 630
MboI GATC 5 cut(s) 205, 215, 342, 493, 628
MboII GAAGA 5 cut(s) 25, 28, 227, 337, 422
MflI RGATCY 2 cut(s) 215, 342
MluCI AATT 2 cut(s) 328, 413
MlyI GAGTC 1 cut(s) 546
MmeI TCCRAC 2 cut(s) 22, 89
MnlI CCTC 7 cut(s) 72, 81, 133, 251, 527, 642, 692
Mph1103I ATGCAT 1 cut(s) 271
MroI TCCGGA 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 569
MseI TTAA 3 cut(s) 308, 416, 639
MslI CAYNNNNRTG 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 5 cut(s) 74, 104, 121, 155, 164
MspR9I CCNGG 4 cut(s) 105, 122, 155, 582
MvaI CCWGG 1 cut(s) 582
MvnI CGCG 2 cut(s) 222, 545
MwoI GCNNNNNNNGC 3 cut(s) 193, 275, 284
NciI CCSGG 3 cut(s) 105, 122, 155
NdeII GATC 5 cut(s) 205, 215, 342, 493, 628
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 2 cut(s) 263, 391
NlaIV GGNNCC 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 271
NspV TTCGAA 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 569
PfeI GAWTC 2 cut(s) 7, 570
PkrI GCNGC 3 cut(s) 63, 189, 579
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
Ppu21I YACGTR 1 cut(s) 562
PshBI ATTAAT 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 186
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 215, 342
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 264
SaqAI TTAA 3 cut(s) 308, 416, 639
SatI GCNGC 3 cut(s) 62, 188, 578
Sau3AI GATC 5 cut(s) 205, 215, 342, 493, 628
SchI GAGTC 1 cut(s) 546
ScrFI CCNGG 4 cut(s) 105, 122, 155, 582
SfaNI GCATC 2 cut(s) 287, 361
SfcI CTRYAG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 2 cut(s) 328, 413
SsiI CCGC 2 cut(s) 188, 543
SspMI CTAG 3 cut(s) 197, 504, 700
StyD4I CCNGG 4 cut(s) 103, 120, 153, 580
TaaI ACNGT 2 cut(s) 49, 433
TaiI ACGT 3 cut(s) 326, 440, 564
TaqI TCGA 4 cut(s) 10, 117, 138, 573
TasI AATT 2 cut(s) 328, 413
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 2 cut(s) 7, 570
Tru1I TTAA 3 cut(s) 308, 416, 639
Tru9I TTAA 3 cut(s) 308, 416, 639
TseI GCWGC 2 cut(s) 61, 577
TspDTI ATGAA 2 cut(s) 621, 666
VspI ATTAAT 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 654
XmnI GAANNNNTTC 1 cut(s) 569
XspI CTAG 3 cut(s) 197, 504, 700
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.