MD15G1007500.v1.1

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009565
Physical Location & Seq
Forward (+)
407861 .. 409594
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1007500.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGCGGAAGCAAAGCACAGAATCCTTAAACTCGAAACAGCTTTAACGTTGTTGTTCGTTCTAGTTTTGAACGGATGCGCGCAGAGCTTGCCACCTTCGAAATACGATGGGTTTTTGTACGGACATCGCCGAGTTGACTCGGATTCGATCCTGATCGAGGCCTTCTTCGACCCGATCTGCCCAGACAGCAGGGACTCCTGGCCGCCCCTCCAACAAGCCGTCGAGTACTACGCCCCTCGCGTCTCGGTGGTAATTCACCTGTTACCCTTACCTTACCACGACAATGCTTTCGTTAGTTCTCGGGCTCTGCACATCGTAAATCAGCTGAAAAAATCTGCCACGTTCCCGTTGTTGGATTGGTTCTTCAAGAATCAGGAGAGGTATTACAATGCTCAAACTCGCAACATTTCCCGGGCTGCTGTTGTAAATGATATAGTGAACTCTGCCACACAAGTGATTGGGAACTCCTATCATAGCGCCCTTGAATCCGGCTTCAATGACCGGAAAACGGACCTCAAAACAAGGGTTTCATTCAAGTATAGCACTTCGAGAGGGGTGTATGGAACGCCTACTTTCTTTGTCAATGGATTTGTACTGCCTGGTTCTGGATCCACTCTAGATCTGAATGGGTGGAAAAAAGTCATTGATCCATTAGTTGTCGGTAACGGGGTGCCAAAGAGCCAAGGAAATATGCACTTCTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin_4

Protein Analysis

236

Amino Acids

26.36

Weight (kDa)

9.16

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 670
AccII CGCG 2 cut(s) 80, 240
AciI CCGC 2 cut(s) 5, 203
AclI AACGTT 1 cut(s) 47
AclWI GGATC 4 cut(s) 142, 603, 616, 641
AcoI YGGCCR 1 cut(s) 200
AfaI GTAC 3 cut(s) 119, 227, 594
AfiI CCNNNNNNNGG 4 cut(s) 157, 352, 508, 605
AgsI TTSAA 5 cut(s) 70, 367, 485, 496, 535
AjnI CCWGG 2 cut(s) 197, 598
AleI CACNNNNGTG 1 cut(s) 452
AluBI AGCT 3 cut(s) 41, 87, 325
AluI AGCT 3 cut(s) 41, 87, 325
Alw26I GTCTC 1 cut(s) 247
AlwI GGATC 4 cut(s) 142, 603, 616, 641
Ama87I CYCGRG 2 cut(s) 300, 411
AoxI GGCC 2 cut(s) 159, 200
ApeKI GCWGC 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AspLEI GCGC 3 cut(s) 80, 82, 479
AspS9I GGNCC 1 cut(s) 511
AsuC2I CCSGG 2 cut(s) 412, 413
AsuHPI GGTGA 1 cut(s) 248
AsuII TTCGAA 1 cut(s) 98
AvaI CYCGRG 2 cut(s) 300, 411
AvaII GGWCC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 403
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 203
BciT130I CCWGG 2 cut(s) 199, 600
BcnI CCSGG 2 cut(s) 412, 413
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 2 cut(s) 62, 617
BfoI RGCGCY 1 cut(s) 480
BglII AGATCT 1 cut(s) 619
BisI GCNGC 2 cut(s) 203, 417
BlsI GCNGC 2 cut(s) 204, 418
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 4 cut(s) 199, 412, 413, 600
Bme18I GGWCC 1 cut(s) 511
BmeT110I CYCGRG 2 cut(s) 300, 411
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 2 cut(s) 610, 672
BmrFI CCNGG 4 cut(s) 199, 412, 413, 600
BmsI GCATC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 412, 413
BsaBI GATNNNNATC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 411, 682
BsaWI WCCGGW 1 cut(s) 501
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 352, 508, 605
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 2 cut(s) 199, 600
BseDI CCNNGG 2 cut(s) 411, 682
BseGI GGATG 1 cut(s) 80
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 4 cut(s) 157, 352, 508, 605
BsePI GCGCGC 1 cut(s) 78
BseXI GCAGC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 2 cut(s) 80, 240
BshFI GGCC 2 cut(s) 161, 202
BshNI GGYRCC 1 cut(s) 670
BsiHKCI CYCGRG 2 cut(s) 300, 411
BsiSI CCGG 3 cut(s) 412, 489, 502
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 4 cut(s) 157, 352, 508, 605
BsmAI GTCTC 1 cut(s) 247
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 2 cut(s) 161, 202
BsoBI CYCGRG 2 cut(s) 300, 411
Bsp119I TTCGAA 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 6 cut(s) 147, 153, 174, 608, 619, 646
BspACI CCGC 2 cut(s) 5, 203
BspANI GGCC 2 cut(s) 161, 202
BspFNI CGCG 2 cut(s) 80, 240
BspLI GGNNCC 2 cut(s) 610, 672
BspPI GGATC 4 cut(s) 142, 603, 616, 641
BspT104I TTCGAA 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 670
BssECI CCNNGG 2 cut(s) 411, 682
BssHII GCGCGC 1 cut(s) 78
BssMI GATC 6 cut(s) 147, 153, 174, 608, 619, 646
BssT1I CCWWGG 1 cut(s) 682
Bst2UI CCWGG 2 cut(s) 199, 600
BstAPI GCANNNNNTGC 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 80, 89
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 80
BstFNI CGCG 2 cut(s) 80, 240
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 3 cut(s) 80, 82, 479
BstKTI GATC 6 cut(s) 150, 156, 177, 611, 622, 649
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 6 cut(s) 147, 153, 174, 608, 619, 646
BstMWI GCNNNNNNNGC 3 cut(s) 84, 88, 186
BstNI CCWGG 2 cut(s) 199, 600
BstSCI CCNGG 4 cut(s) 197, 410, 411, 598
BstUI CGCG 2 cut(s) 80, 240
BstV1I GCAGC 1 cut(s) 403
BstX2I RGATCY 2 cut(s) 608, 619
BstYI RGATCY 2 cut(s) 608, 619
BsuRI GGCC 2 cut(s) 161, 202
BtgZI GCGATG 1 cut(s) 110
BtsCI GGATG 1 cut(s) 80
Cac8I GCNNGC 2 cut(s) 80, 89
CfoI GCGC 3 cut(s) 80, 82, 479
Cfr13I GGNCC 1 cut(s) 511
Cfr9I CCCGGG 1 cut(s) 411
CseI GACGC 1 cut(s) 229
Csp6I GTAC 3 cut(s) 118, 226, 593
CviQI GTAC 3 cut(s) 118, 226, 593
DpnI GATC 6 cut(s) 149, 155, 176, 610, 621, 648
DpnII GATC 6 cut(s) 147, 153, 174, 608, 619, 646
EaeI YGGCCR 1 cut(s) 200
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 682
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 511
Eco88I CYCGRG 2 cut(s) 300, 411
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoRII CCWGG 2 cut(s) 197, 598
EcoT14I CCWWGG 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 682
Esp3I CGTCTC 1 cut(s) 247
FaiI YATR 5 cut(s) 434, 474, 540, 561, 692
FaqI GGGAC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 203, 417
FokI GGATG 1 cut(s) 87
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 2 cut(s) 203, 417
FspBI CTAG 2 cut(s) 62, 617
GlaI GCGC 3 cut(s) 79, 81, 478
GluI GCNGC 2 cut(s) 203, 417
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 2 cut(s) 161, 202
HapII CCGG 3 cut(s) 412, 489, 502
HgaI GACGC 1 cut(s) 229
HhaI GCGC 3 cut(s) 80, 82, 479
Hin6I GCGC 3 cut(s) 78, 80, 477
HinP1I GCGC 3 cut(s) 78, 80, 477
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 6 cut(s) 21, 137, 143, 194, 370, 485
HpaII CCGG 3 cut(s) 412, 489, 502
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 2 cut(s) 136, 439
Hpy188I TCNGA 2 cut(s) 142, 624
Hpy188III TCNNGA 6 cut(s) 151, 367, 374, 549, 606, 617
Hpy8I GTNNAC 2 cut(s) 136, 439
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 2 cut(s) 105, 172
HpyCH4IV ACGT 2 cut(s) 47, 341
HpyCH4V TGCA 2 cut(s) 310, 694
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 88, 186
HpySE526I ACGT 2 cut(s) 47, 341
HspAI GCGC 3 cut(s) 78, 80, 477
Kzo9I GATC 6 cut(s) 147, 153, 174, 608, 619, 646
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 1 cut(s) 65
MaeI CTAG 2 cut(s) 62, 617
MaeII ACGT 2 cut(s) 47, 341
MaeIII GTNAC 2 cut(s) 261, 662
MalI GATC 6 cut(s) 149, 155, 176, 610, 621, 648
MboI GATC 6 cut(s) 147, 153, 174, 608, 619, 646
MboII GAAGA 3 cut(s) 157, 355, 691
MflI RGATCY 2 cut(s) 608, 619
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 1 cut(s) 252
MlyI GAGTC 2 cut(s) 131, 188
MmeI TCCRAC 2 cut(s) 235, 333
MnlI CCTC 6 cut(s) 151, 218, 246, 372, 524, 545
MseI TTAA 2 cut(s) 27, 44
MslI CAYNNNNRTG 2 cut(s) 282, 452
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 3 cut(s) 412, 489, 502
MspR9I CCNGG 4 cut(s) 199, 412, 413, 600
MvaI CCWGG 2 cut(s) 199, 600
MvnI CGCG 2 cut(s) 80, 240
MwoI GCNNNNNNNGC 3 cut(s) 84, 88, 186
NciI CCSGG 2 cut(s) 412, 413
NdeII GATC 6 cut(s) 147, 153, 174, 608, 619, 646
NlaIV GGNNCC 2 cut(s) 610, 672
NmeAIII GCCGAG 1 cut(s) 155
NspV TTCGAA 1 cut(s) 98
OliI CACNNNNGTG 1 cut(s) 452
PauI GCGCGC 1 cut(s) 78
PceI AGGCCT 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 4 cut(s) 21, 143, 370, 485
PkrI GCNGC 2 cut(s) 204, 418
PleI GAGTC 2 cut(s) 131, 188
PpsI GAGTC 2 cut(s) 131, 188
Psp1406I AACGTT 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 197, 598
PspGI CCWGG 2 cut(s) 197, 598
PspN4I GGNNCC 2 cut(s) 610, 672
PspPI GGNCC 1 cut(s) 511
PsuI RGATCY 2 cut(s) 608, 619
PteI GCGCGC 1 cut(s) 78
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 3 cut(s) 119, 227, 594
RsaNI GTAC 3 cut(s) 118, 226, 593
RseI CAYNNNNRTG 2 cut(s) 282, 452
SaqAI TTAA 2 cut(s) 27, 44
SatI GCNGC 2 cut(s) 203, 417
Sau3AI GATC 6 cut(s) 147, 153, 174, 608, 619, 646
Sau96I GGNCC 1 cut(s) 511
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 2 cut(s) 131, 188
ScrFI CCNGG 4 cut(s) 199, 412, 413, 600
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 98
SinI GGWCC 1 cut(s) 511
SmaI CCCGGG 1 cut(s) 413
SmiMI CAYNNNNRTG 2 cut(s) 282, 452
Sse9I AATT 1 cut(s) 252
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 2 cut(s) 5, 203
SspMI CTAG 2 cut(s) 62, 617
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 4 cut(s) 197, 410, 411, 598
StyI CCWWGG 1 cut(s) 682
TaiI ACGT 2 cut(s) 50, 344
TaqI TCGA 7 cut(s) 33, 98, 146, 156, 168, 222, 548
TasI AATT 1 cut(s) 252
TatI WGTACW 2 cut(s) 225, 592
TauI GCSGC 1 cut(s) 205
TfiI GAWTC 4 cut(s) 21, 143, 370, 485
Tru1I TTAA 2 cut(s) 27, 44
Tru9I TTAA 2 cut(s) 27, 44
TseI GCWGC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 3 cut(s) 87, 135, 524
TspMI CCCGGG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 511
XagI CCTNNNNNAGG 1 cut(s) 155
XbaI TCTAGA 1 cut(s) 616
XmaI CCCGGG 1 cut(s) 411
XspI CTAG 2 cut(s) 62, 617
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.