pycom08g00690

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009565
Physical Location & Seq
Forward (+)
554837 .. 557162
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00690.4

Sequence Viewer

Length: 945 bp
ATGTTAGAGATCTTAGGTTCGATTCTCGCCAAAGCTTATCCCTTCCTCTCAGTGTTGATAATATCGTTTGTCAAAAAAAAAAAAAAAAACAATAACTTCTCAAAAAAAAAAGTTTTGTCAAAATCCAGGGAAGGTACATTTCCACAGGCGGTTACATGCGGTCTTGGTGCAGCTCAGGTGATTTGCCTTCATACGGTCCACTCGAGGAAACAGAGAGACAAAAGCGGAAGAAAAAGAATGGCGGAATCAAAGCACAGAATCCTTAAACTCAAAACAGCTTTAACGTTGTTGTTCGTGCTGGTTTTGAGCGGATGCGCGCAGAGCTTGCCCCCTTCAAAATACGATGGGTTTTTGTACGGACATCACCTAGTTGACTCCGATTCGATCCTGATCGAAGCTTTCTTCGACCCGGTCTGCCCAGACAGCAGGGATGCCTGGCCGCCCCTCAAACAAGTCGTCGAGCACTACGCCCCTCGCGTCACTGTGGCAATTCACCTCTTACCGTTGCCTTACCATGACAATGCTTTCGTTACTTCTCGGGCTCTTCACATCGTAAATCAGCTGAAACCTTCTGCTACATTCCCATTGTTGGAGTGGTTCTTCAAGAATCAGGAGAGGTATTATAATGCTCAAACTCGCAACATTTCCAGGGTTGCTGTTGTAAATGACCTAGTGAACTCTGTCACAGAAGTGATTGGGAACTCCTATCACAGCGCCCTTGAATTCGGCTTCAGTGACCTGAAAACGGATCTGAAAACAAGGGTTTCCTTCAAGTTTAGCACTTCAAGAGGGGTGTACGGAACACCTACTTTCTTTGTCAATGGATTTGTATTGCCAGATGCTGGCTCCAGTCTGGATTTTAACGGGTGGAAAAAAATCATTGATCCGTTAGTTGTTGGTAACGGGGCGCCAAAGAGCCAAGAAAATGTGCAATTCTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin_4

Protein Analysis

315

Amino Acids

35.16

Weight (kDa)

9.7

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AccB1I GGYRCC 1 cut(s) 907
AccB7I CCANNNNNTGG 1 cut(s) 842
AccBSI CCGCTC 1 cut(s) 309
AccII CGCG 2 cut(s) 317, 477
AciI CCGC 6 cut(s) 149, 159, 225, 242, 309, 440
AclI AACGTT 1 cut(s) 284
AclWI GGATC 3 cut(s) 379, 756, 878
AcoI YGGCCR 1 cut(s) 437
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 1 cut(s) 715
AcyI GRCGYC 1 cut(s) 908
AfaI GTAC 3 cut(s) 136, 356, 797
AfiI CCNNNNNNNGG 4 cut(s) 193, 589, 745, 842
AgsI TTSAA 5 cut(s) 336, 604, 722, 772, 786
AjnI CCWGG 3 cut(s) 125, 434, 647
AleI CACNNNNGTG 1 cut(s) 689
AluBI AGCT 6 cut(s) 35, 173, 278, 324, 398, 562
AluI AGCT 6 cut(s) 35, 173, 278, 324, 398, 562
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 3 cut(s) 379, 756, 878
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 2 cut(s) 202, 537
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 722
ArsI GACNNNNNNTTYG 4 cut(s) 441, 473, 509, 541
AspLEI GCGC 4 cut(s) 317, 319, 716, 910
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 3 cut(s) 190, 356, 485
AvaI CYCGRG 2 cut(s) 202, 537
AvaII GGWCC 1 cut(s) 196
BanI GGYRCC 1 cut(s) 907
BanII GRGCYC 1 cut(s) 544
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 182
BccI CCATC 1 cut(s) 338
BciT130I CCWGG 3 cut(s) 127, 436, 649
BcnI CCSGG 1 cut(s) 410
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 2 cut(s) 368, 671
BfoI RGCGCY 2 cut(s) 717, 911
BglII AGATCT 1 cut(s) 9
BisI GCNGC 2 cut(s) 171, 440
BlsI GCNGC 2 cut(s) 172, 441
Bme1390I CCNGG 4 cut(s) 127, 410, 436, 649
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 2 cut(s) 202, 537
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 2 cut(s) 847, 909
BmrFI CCNGG 4 cut(s) 127, 410, 436, 649
BmsI GCATC 3 cut(s) 302, 421, 829
BpmI CTGGAG 1 cut(s) 832
Bpu10I CCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 410
BsaBI GATNNNNATC 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 908
BsaJI CCNNGG 2 cut(s) 126, 648
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 589, 745, 842
Bse1I ACTGG 1 cut(s) 849
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 3 cut(s) 127, 436, 649
BseDI CCNNGG 2 cut(s) 126, 648
BseGI GGATG 2 cut(s) 317, 436
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 4 cut(s) 193, 589, 745, 842
BseMII CTCAG 2 cut(s) 63, 188
BseNI ACTGG 1 cut(s) 849
BsePI GCGCGC 1 cut(s) 315
BseXI GCAGC 1 cut(s) 182
BsgI GTGCAG 1 cut(s) 189
Bsh1236I CGCG 2 cut(s) 317, 477
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 907
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 2 cut(s) 202, 537
BsiSI CCGG 1 cut(s) 410
BslI CCNNNNNNNGG 4 cut(s) 193, 589, 745, 842
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 1 cut(s) 439
BsoBI CYCGRG 2 cut(s) 202, 537
Bsp1286I GDGCHC 2 cut(s) 465, 544
Bsp143I GATC 5 cut(s) 9, 384, 390, 748, 883
BspACI CCGC 6 cut(s) 149, 159, 225, 242, 309, 440
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 2 cut(s) 62, 187
BspFNI CGCG 2 cut(s) 317, 477
BspLI GGNNCC 2 cut(s) 847, 909
BspPI GGATC 3 cut(s) 379, 756, 878
BspQI GCTCTTC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 907
BsrBI CCGCTC 1 cut(s) 309
BsrI ACTGG 1 cut(s) 849
BssECI CCNNGG 2 cut(s) 126, 648
BssHII GCGCGC 1 cut(s) 315
BssMI GATC 5 cut(s) 9, 384, 390, 748, 883
BssNI GRCGYC 1 cut(s) 908
Bst2UI CCWGG 3 cut(s) 127, 436, 649
Bst4CI ACNGT 3 cut(s) 196, 484, 504
Bst6I CTCTTC 1 cut(s) 549
BstACI GRCGYC 1 cut(s) 908
BstAPI GCANNNNNTGC 1 cut(s) 325
BstC8I GCNNGC 3 cut(s) 317, 326, 844
BstDEI CTNAG 3 cut(s) 13, 49, 174
BstF5I GGATG 2 cut(s) 317, 436
BstFNI CGCG 2 cut(s) 317, 477
BstH2I RGCGCY 2 cut(s) 717, 911
BstHHI GCGC 4 cut(s) 317, 319, 716, 910
BstKTI GATC 5 cut(s) 12, 387, 393, 751, 886
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 5 cut(s) 9, 384, 390, 748, 883
BstMWI GCNNNNNNNGC 3 cut(s) 321, 325, 423
BstNI CCWGG 3 cut(s) 127, 436, 649
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 4 cut(s) 125, 408, 434, 647
BstUI CGCG 2 cut(s) 317, 477
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 9, 748
BstYI RGATCY 2 cut(s) 9, 748
BsuRI GGCC 1 cut(s) 439
BtsCI GGATG 2 cut(s) 317, 436
BtsIMutI CAGTG 3 cut(s) 57, 480, 739
Cac8I GCNNGC 3 cut(s) 317, 326, 844
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 4 cut(s) 317, 319, 716, 910
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 1 cut(s) 466
Csp6I GTAC 3 cut(s) 135, 355, 796
CviAII CATG 2 cut(s) 156, 515
CviQI GTAC 3 cut(s) 135, 355, 796
DdeI CTNAG 3 cut(s) 13, 49, 174
DinI GGCGCC 1 cut(s) 909
DpnI GATC 5 cut(s) 11, 386, 392, 750, 885
DpnII GATC 5 cut(s) 9, 384, 390, 748, 883
EaeI YGGCCR 1 cut(s) 437
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
EciI GGCGGA 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 715
Eco88I CYCGRG 2 cut(s) 202, 537
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 3 cut(s) 125, 434, 647
EcoT38I GRGCYC 1 cut(s) 544
EgeI GGCGCC 1 cut(s) 909
EheI GGCGCC 1 cut(s) 909
FaeI CATG 2 cut(s) 159, 518
FaiI YATR 4 cut(s) 157, 192, 516, 624
FatI CATG 2 cut(s) 155, 514
Fnu4HI GCNGC 2 cut(s) 171, 440
FokI GGATG 2 cut(s) 324, 443
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 2 cut(s) 171, 440
FspBI CTAG 2 cut(s) 368, 671
GlaI GCGC 4 cut(s) 316, 318, 715, 909
GluI GCNGC 2 cut(s) 171, 440
GsuI CTGGAG 1 cut(s) 832
HaeII RGCGCY 2 cut(s) 717, 911
HaeIII GGCC 1 cut(s) 439
HapII CCGG 1 cut(s) 410
HgaI GACGC 1 cut(s) 466
HhaI GCGC 4 cut(s) 317, 319, 716, 910
Hin1I GRCGYC 1 cut(s) 908
Hin1II CATG 2 cut(s) 159, 518
Hin6I GCGC 4 cut(s) 315, 317, 714, 908
HinP1I GCGC 4 cut(s) 315, 317, 714, 908
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 2 cut(s) 33, 396
HinfI GANTC 6 cut(s) 22, 245, 258, 374, 380, 607
HpaII CCGG 1 cut(s) 410
HphI GGTGA 3 cut(s) 190, 356, 485
Hpy166II GTNNAC 4 cut(s) 199, 373, 676, 796
Hpy188I TCNGA 2 cut(s) 379, 753
Hpy188III TCNNGA 5 cut(s) 388, 604, 611, 786, 854
Hpy8I GTNNAC 4 cut(s) 199, 373, 676, 796
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 6 cut(s) 52, 125, 197, 342, 579, 778
HpyCH4III ACNGT 3 cut(s) 196, 484, 504
HpyCH4IV ACGT 1 cut(s) 284
HpyCH4V TGCA 2 cut(s) 170, 931
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 325, 423
HpyF3I CTNAG 3 cut(s) 13, 49, 174
HpySE526I ACGT 1 cut(s) 284
Hsp92I GRCGYC 1 cut(s) 908
Hsp92II CATG 2 cut(s) 159, 518
HspAI GCGC 4 cut(s) 315, 317, 714, 908
KasI GGCGCC 1 cut(s) 907
Kzo9I GATC 5 cut(s) 9, 384, 390, 748, 883
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 1 cut(s) 851
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 3 cut(s) 302, 421, 829
MaeI CTAG 2 cut(s) 368, 671
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 6 cut(s) 151, 478, 529, 682, 734, 899
MalI GATC 5 cut(s) 11, 386, 392, 750, 885
MbiI CCGCTC 1 cut(s) 309
MboI GATC 5 cut(s) 9, 384, 390, 748, 883
MboII GAAGA 5 cut(s) 240, 394, 536, 592, 928
MflI RGATCY 2 cut(s) 9, 748
MhlI GDGCHC 2 cut(s) 465, 544
MluCI AATT 3 cut(s) 489, 722, 932
Mly113I GGCGCC 1 cut(s) 908
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 7 cut(s) 56, 198, 455, 483, 506, 609, 782
MseI TTAA 3 cut(s) 264, 281, 861
MslI CAYNNNNRTG 2 cut(s) 519, 689
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 4 cut(s) 127, 410, 436, 649
MvaI CCWGG 3 cut(s) 127, 436, 649
MvnI CGCG 2 cut(s) 317, 477
MwoI GCNNNNNNNGC 3 cut(s) 321, 325, 423
NarI GGCGCC 1 cut(s) 908
NciI CCSGG 1 cut(s) 410
NdeII GATC 5 cut(s) 9, 384, 390, 748, 883
NlaIII CATG 2 cut(s) 159, 518
NlaIV GGNNCC 2 cut(s) 847, 909
NmuCI GTSAC 3 cut(s) 478, 682, 734
NspI RCATGY 1 cut(s) 159
OliI CACNNNNGTG 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 202
PauI GCGCGC 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 474
PfeI GAWTC 5 cut(s) 22, 245, 258, 380, 607
PflFI GACNNNGTC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 842
PkrI GCNGC 2 cut(s) 172, 441
PleI GAGTC 1 cut(s) 368
PluTI GGCGCC 1 cut(s) 911
PpsI GAGTC 1 cut(s) 368
PsiI TTATAA 1 cut(s) 624
Psp1406I AACGTT 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 125, 434, 647
PspGI CCWGG 3 cut(s) 125, 434, 647
PspN4I GGNNCC 2 cut(s) 847, 909
PspPI GGNCC 1 cut(s) 196
PspXI VCTCGAGB 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 2 cut(s) 9, 748
PsyI GACNNNGTC 1 cut(s) 410
PteI GCGCGC 1 cut(s) 315
PvuII CAGCTG 1 cut(s) 562
RsaI GTAC 3 cut(s) 136, 356, 797
RsaNI GTAC 3 cut(s) 135, 355, 796
RseI CAYNNNNRTG 2 cut(s) 519, 689
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 3 cut(s) 264, 281, 861
SatI GCNGC 2 cut(s) 171, 440
Sau3AI GATC 5 cut(s) 9, 384, 390, 748, 883
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 4 cut(s) 127, 410, 436, 649
SduI GDGCHC 2 cut(s) 465, 544
SfaNI GCATC 3 cut(s) 302, 421, 829
SfoI GGCGCC 1 cut(s) 909
Sfr274I CTCGAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 202
SmiMI CAYNNNNRTG 2 cut(s) 519, 689
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 3 cut(s) 489, 722, 932
SsiI CCGC 6 cut(s) 149, 159, 225, 242, 309, 440
SspDI GGCGCC 1 cut(s) 907
SspMI CTAG 2 cut(s) 368, 671
StyD4I CCNGG 4 cut(s) 125, 408, 434, 647
TaaI ACNGT 3 cut(s) 196, 484, 504
TaiI ACGT 1 cut(s) 287
TaqI TCGA 6 cut(s) 20, 203, 383, 393, 405, 459
TasI AATT 3 cut(s) 489, 722, 932
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 5 cut(s) 22, 245, 258, 380, 607
Tru1I TTAA 3 cut(s) 264, 281, 861
Tru9I TTAA 3 cut(s) 264, 281, 861
TscAI CASTG 3 cut(s) 57, 487, 739
TseFI GTSAC 3 cut(s) 478, 682, 734
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 3 cut(s) 478, 682, 734
TspDTI ATGAA 1 cut(s) 179
TspGWI ACGGA 4 cut(s) 372, 761, 813, 876
TspRI CASTG 3 cut(s) 57, 487, 739
Tth111I GACNNNGTC 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 842
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 722
XceI RCATGY 1 cut(s) 159
XcmI CCANNNNNNNNNTGG 1 cut(s) 591
XhoI CTCGAG 1 cut(s) 202
XspI CTAG 2 cut(s) 368, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.