Rroxscaffold_7G00164620

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009565
Physical Location & Seq
Forward (+)
6561979 .. 6563777
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164620.1

Sequence Viewer

Length: 699 bp
ATGGAGGAAGCAAAGCACAAAATCGTCGTAGTACAAACCGCTTTGCTGTTGCTGTTGGTTTTGGTTTTAAATGGGTATGCACAGAACTTGCCCCCTGCCAAGTACGATGGGTTCTTGTACGAGCATAGCCTGATTGACCCGGATTCGATTCTCATCGAAGCTTTCTTTGACCCGGTCTGCCCGGACAGTAGAGATTCCTGGCCACCCCTCAAACAGGCCCTAGCCTACTACGGCCCTCACGTCACTCTTGCTGTGCACCTATTACCGTTGCCTTACCATGACAACGCTTTTGTTGCTTCTCGAGCTATACACATCGTAAATAAGCTGAAACCTTCTGCTAAGTTTTCCGTGTTGGAGTGGTTCTTTAAGAATCAGGAGAGGTATTACAATGCTCAAACGCGCAACATTTCTAGGGCTTCCATTGTGGATGACTTAGTGAAGCAACTAACAGCAGTACTTGGAAACTCTTATCATAGTTCCCTGGAATCAGGCTTTAATGACCGGAAAACTGATCTCACAACAAGGGTTTCCTTTAAGTATAGTACTTCAAGAGGGGTGTATGGAACGCCTACTTTCTACGTAAATGGATTTGTATTGCCTGATTCTGGCGATGCTCTAGATTTTAACGGGTGGAAAAAAGTGATTGACCCTTTAGTTACGGCACCAAAGAAACAGGAAAATTTGCATTTCTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin_4

Protein Analysis

232

Amino Acids

26.23

Weight (kDa)

7.1

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_4 PF13462 52 - 216 9.1e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 697
AccB1I GGYRCC 1 cut(s) 661
AccII CGCG 1 cut(s) 400
AciI CCGC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 200
AcsI RAATTY 1 cut(s) 679
AfaI GTAC 5 cut(s) 33, 104, 119, 456, 544
AfiI CCNNNNNNNGG 2 cut(s) 214, 605
AgsI TTSAA 1 cut(s) 549
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 2 cut(s) 197, 480
AluBI AGCT 3 cut(s) 161, 305, 325
AluI AGCT 3 cut(s) 161, 305, 325
Alw21I GWGCWC 1 cut(s) 258
Alw44I GTGCAC 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 3 cut(s) 200, 216, 232
ApaLI GTGCAC 1 cut(s) 254
ApoI RAATTY 1 cut(s) 679
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 217, 233
AsuC2I CCSGG 3 cut(s) 140, 173, 182
AvaI CYCGRG 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 202
BanI GGYRCC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 247, 675
BciT130I CCWGG 2 cut(s) 199, 482
BcnI CCSGG 3 cut(s) 140, 173, 182
BfaI CTAG 3 cut(s) 221, 411, 617
BmcAI AGTACT 2 cut(s) 456, 544
Bme1390I CCNGG 5 cut(s) 140, 173, 182, 199, 482
BmeT110I CYCGRG 1 cut(s) 300
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 2 cut(s) 217, 233
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 5 cut(s) 140, 173, 182, 199, 482
BmsI GCATC 1 cut(s) 601
BpuMI CCSGG 3 cut(s) 140, 173, 182
BsaAI YACGTR 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 480
BsaWI WCCGGW 1 cut(s) 501
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 605
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 2 cut(s) 199, 482
BseDI CCNNGG 1 cut(s) 480
BseGI GGATG 1 cut(s) 433
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 2 cut(s) 214, 605
BseSI GKGCMC 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 3 cut(s) 202, 218, 234
BshNI GGYRCC 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 4 cut(s) 140, 173, 182, 502
BslI CCNNNNNNNGG 2 cut(s) 214, 605
BsnI GGCC 3 cut(s) 202, 218, 234
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 1 cut(s) 511
BspACI CCGC 1 cut(s) 39
BspANI GGCC 3 cut(s) 202, 218, 234
BspFNI CGCG 1 cut(s) 400
BspLI GGNNCC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 661
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 1 cut(s) 511
Bst2UI CCWGG 2 cut(s) 199, 482
Bst4CI ACNGT 2 cut(s) 188, 267
BstBAI YACGTR 1 cut(s) 580
BstDEI CTNAG 2 cut(s) 339, 433
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 1 cut(s) 400
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 1 cut(s) 514
BstMBI GATC 1 cut(s) 511
BstMWI GCNNNNNNNGC 2 cut(s) 293, 302
BstNI CCWGG 2 cut(s) 199, 482
BstSCI CCNGG 5 cut(s) 138, 171, 180, 197, 480
BstSLI GKGCMC 1 cut(s) 258
BstSNI TACGTA 1 cut(s) 580
BstUI CGCG 1 cut(s) 400
BsuRI GGCC 3 cut(s) 202, 218, 234
BtgZI GCGATG 1 cut(s) 624
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 433
CfoI GCGC 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 217, 233
Csp6I GTAC 5 cut(s) 32, 103, 118, 455, 543
CviAII CATG 1 cut(s) 278
CviQI GTAC 5 cut(s) 32, 103, 118, 455, 543
DdeI CTNAG 2 cut(s) 339, 433
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
DraI TTTAAA 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 200
Eco105I TACGTA 1 cut(s) 580
Eco88I CYCGRG 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 2 cut(s) 197, 480
FaeI CATG 1 cut(s) 281
FaiI YATR 8 cut(s) 78, 126, 279, 308, 474, 540, 561, 697
FatI CATG 1 cut(s) 277
FokI GGATG 1 cut(s) 440
FspBI CTAG 3 cut(s) 221, 411, 617
GlaI GCGC 1 cut(s) 401
HaeIII GGCC 3 cut(s) 202, 218, 234
HapII CCGG 4 cut(s) 140, 173, 182, 502
HhaI GCGC 1 cut(s) 402
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 6 cut(s) 143, 148, 194, 370, 485, 602
HpaII CCGG 4 cut(s) 140, 173, 182, 502
Hpy166II GTNNAC 1 cut(s) 256
Hpy188III TCNNGA 4 cut(s) 300, 374, 549, 617
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 2 cut(s) 188, 267
HpyCH4IV ACGT 2 cut(s) 240, 579
HpyCH4V TGCA 3 cut(s) 80, 256, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 302
HpyF3I CTNAG 2 cut(s) 339, 433
HpySE526I ACGT 2 cut(s) 240, 579
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 1 cut(s) 400
Kzo9I GATC 1 cut(s) 511
LweI GCATC 1 cut(s) 601
MaeI CTAG 3 cut(s) 221, 411, 617
MaeII ACGT 2 cut(s) 240, 579
MaeIII GTNAC 2 cut(s) 241, 655
MalI GATC 1 cut(s) 513
MboI GATC 1 cut(s) 511
MboII GAAGA 1 cut(s) 682
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 202
MluCI AATT 1 cut(s) 679
MluNI TGGCCA 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 4 cut(s) 218, 246, 372, 545
Mox20I TGGCCA 1 cut(s) 202
MscI TGGCCA 1 cut(s) 202
MseI TTAA 5 cut(s) 68, 366, 495, 534, 624
Msp20I TGGCCA 1 cut(s) 202
MspI CCGG 4 cut(s) 140, 173, 182, 502
MspR9I CCNGG 5 cut(s) 140, 173, 182, 199, 482
MvaI CCWGG 2 cut(s) 199, 482
MvnI CGCG 1 cut(s) 400
MwoI GCNNNNNNNGC 2 cut(s) 293, 302
NciI CCSGG 3 cut(s) 140, 173, 182
NdeII GATC 1 cut(s) 511
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 241
PaeR7I CTCGAG 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 6 cut(s) 143, 148, 194, 370, 485, 602
PflFI GACNNNGTC 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 580
PsiI TTATAA 1 cut(s) 697
Psp6I CCWGG 2 cut(s) 197, 480
PspGI CCWGG 2 cut(s) 197, 480
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 2 cut(s) 217, 233
PsrI GAACNNNNNNTAC 2 cut(s) 95, 127
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 5 cut(s) 33, 104, 119, 456, 544
RsaNI GTAC 5 cut(s) 32, 103, 118, 455, 543
SaqAI TTAA 5 cut(s) 68, 366, 495, 534, 624
Sau3AI GATC 1 cut(s) 511
Sau96I GGNCC 2 cut(s) 217, 233
ScaI AGTACT 2 cut(s) 456, 544
ScrFI CCNGG 5 cut(s) 140, 173, 182, 199, 482
SduI GDGCHC 1 cut(s) 258
SetI ASST 8 cut(s) 163, 243, 261, 307, 327, 334, 383, 582
SfaNI GCATC 1 cut(s) 601
Sfr274I CTCGAG 1 cut(s) 300
SlaI CTCGAG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SnaBI TACGTA 1 cut(s) 580
Sse9I AATT 1 cut(s) 679
SsiI CCGC 1 cut(s) 39
SspMI CTAG 3 cut(s) 221, 411, 617
StyD4I CCNGG 5 cut(s) 138, 171, 180, 197, 480
TaaI ACNGT 2 cut(s) 188, 267
TaiI ACGT 2 cut(s) 243, 582
TaqI TCGA 3 cut(s) 146, 156, 301
TasI AATT 1 cut(s) 679
TatI WGTACW 3 cut(s) 31, 454, 542
TfiI GAWTC 6 cut(s) 143, 148, 194, 370, 485, 602
Tru1I TTAA 5 cut(s) 68, 366, 495, 534, 624
Tru9I TTAA 5 cut(s) 68, 366, 495, 534, 624
TseFI GTSAC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 241
TspGWI ACGGA 1 cut(s) 337
Tth111I GACNNNGTC 1 cut(s) 173
VneI GTGCAC 1 cut(s) 254
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 1 cut(s) 679
XbaI TCTAGA 1 cut(s) 616
XhoI CTCGAG 1 cut(s) 300
XspI CTAG 3 cut(s) 221, 411, 617
ZrmI AGTACT 2 cut(s) 456, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.