RchiOBHm_Chr6g0303571

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009565
Physical Location & Seq
Reverse (-)
63268296 .. 63270205
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27273

Sequence Viewer

Length: 693 bp
ATGGAGGAAGCAAAGCACAAAATCGTCATAGTACAAACAGCTTTGCTGTTGCTGTTGGTTTTAAATGGGTATGCACAGAACTTGCCCCCTGCCAAGTACGATGGGTTCTTGTACGAGCATCGCCTGATTGACCCGGATTCGATTCTCATCGAAGCTTTCTTTGACCCGGTCTGCCCGGACAGCAGAGATTCCTGGCCACCCCTCAAACAGGCCCTAGCCTACTACGGCCCTCACGTCAGTCTTGCTGTGCACCTATTACCGTTGCCTTACCATGACAACGCTTTTGTTGCTTCTCGTGCTATACAAATTGTAAATAAGCTGAAACCTTCTGCTACGTTTGCCGTGTTGGAGTGGTTCTTCAAGAATCAGGAGAGGTATTACAATGCTCAAACGCGCAACATTTCTAGGGCTTCCATTGTGGATGACTTAGTGAAGCAACTAACAGCAGTACTAGGGAACTCTTATCATAGTTCCCTGGAATCAGGCTTTAATGACCGGAAAACTGATCTCACAACAAGGGTTTCCTTTAAGTATAGTACTTCAAGAGGGGTGTATGGAACGCCTACTTTCTATGTAAATGGATTTGTATTGCCTGATTCTGGCAATGCTCTAGATTTTAACGGGTGGAAAAAAGTGATTGACCCATTAGTTACGGCACCAAAGAAACAGGAAAATTTGCATTTCTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin_4

Protein Analysis

230

Amino Acids

26.04

Weight (kDa)

7.82

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 655
AccII CGCG 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 194
AcsI RAATTY 1 cut(s) 673
AfaI GTAC 5 cut(s) 33, 98, 113, 450, 538
AfiI CCNNNNNNNGG 2 cut(s) 208, 599
AgsI TTSAA 2 cut(s) 361, 543
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 2 cut(s) 191, 474
AluBI AGCT 3 cut(s) 41, 155, 319
AluI AGCT 3 cut(s) 41, 155, 319
Alw21I GWGCWC 1 cut(s) 252
Alw44I GTGCAC 1 cut(s) 248
AoxI GGCC 3 cut(s) 194, 210, 226
ApaLI GTGCAC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 673
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AspLEI GCGC 1 cut(s) 396
AspS9I GGNCC 2 cut(s) 211, 227
AsuC2I CCSGG 3 cut(s) 134, 167, 176
BaeGI GKGCMC 1 cut(s) 252
BalI TGGCCA 1 cut(s) 196
BanI GGYRCC 1 cut(s) 655
BauI CACGAG 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 252
BccI CCATC 1 cut(s) 95
BceAI ACGGC 3 cut(s) 241, 326, 669
BciT130I CCWGG 2 cut(s) 193, 476
BcnI CCSGG 3 cut(s) 134, 167, 176
BfaI CTAG 4 cut(s) 215, 405, 452, 611
BmcAI AGTACT 2 cut(s) 450, 538
Bme1390I CCNGG 5 cut(s) 134, 167, 176, 193, 476
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 211, 227
BmiI GGNNCC 1 cut(s) 657
BmrFI CCNGG 5 cut(s) 134, 167, 176, 193, 476
BmsI GCATC 1 cut(s) 127
BpuMI CCSGG 3 cut(s) 134, 167, 176
BsaBI GATNNNNATC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 599
Bse3DI GCAATG 1 cut(s) 610
Bse8I GATNNNNATC 1 cut(s) 146
BseBI CCWGG 2 cut(s) 193, 476
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 1 cut(s) 427
BseJI GATNNNNATC 1 cut(s) 146
BseLI CCNNNNNNNGG 2 cut(s) 208, 599
BseMI GCAATG 1 cut(s) 610
BseSI GKGCMC 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 3 cut(s) 196, 212, 228
BshNI GGYRCC 1 cut(s) 655
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 4 cut(s) 134, 167, 176, 496
BslI CCNNNNNNNGG 2 cut(s) 208, 599
BsnI GGCC 3 cut(s) 196, 212, 228
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 505
BspANI GGCC 3 cut(s) 196, 212, 228
BspFNI CGCG 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 1 cut(s) 505
BssSI CACGAG 1 cut(s) 294
Bst2BI CACGAG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 193, 476
Bst4CI ACNGT 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 427
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 394
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 1 cut(s) 508
BstMBI GATC 1 cut(s) 505
BstMWI GCNNNNNNNGC 4 cut(s) 180, 287, 296, 338
BstNI CCWGG 2 cut(s) 193, 476
BstSCI CCNGG 5 cut(s) 132, 165, 174, 191, 474
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 394
BsuRI GGCC 3 cut(s) 196, 212, 228
BtgZI GCGATG 1 cut(s) 104
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 1 cut(s) 427
CfoI GCGC 1 cut(s) 396
Cfr13I GGNCC 2 cut(s) 211, 227
Csp6I GTAC 5 cut(s) 32, 97, 112, 449, 537
CviAII CATG 1 cut(s) 272
CviJI RGCY 9 cut(s) 41, 155, 196, 212, 218, 228, 319, 410, 486
CviKI_1 RGCY 9 cut(s) 41, 155, 196, 212, 218, 228, 319, 410, 486
CviQI GTAC 5 cut(s) 32, 97, 112, 449, 537
DdeI CTNAG 1 cut(s) 427
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
DraI TTTAAA 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 194
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 2 cut(s) 191, 474
FaeI CATG 1 cut(s) 275
FaiI YATR 9 cut(s) 29, 72, 273, 302, 468, 534, 555, 573, 691
FatI CATG 1 cut(s) 271
FokI GGATG 1 cut(s) 434
FspBI CTAG 4 cut(s) 215, 405, 452, 611
GlaI GCGC 1 cut(s) 395
HaeIII GGCC 3 cut(s) 196, 212, 228
HapII CCGG 4 cut(s) 134, 167, 176, 496
HhaI GCGC 1 cut(s) 396
Hin1II CATG 1 cut(s) 275
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 153
HinfI GANTC 6 cut(s) 137, 142, 188, 364, 479, 596
HpaII CCGG 4 cut(s) 134, 167, 176, 496
Hpy166II GTNNAC 1 cut(s) 250
Hpy188III TCNNGA 4 cut(s) 361, 368, 543, 611
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 1 cut(s) 261
HpyCH4IV ACGT 2 cut(s) 234, 335
HpyCH4V TGCA 3 cut(s) 74, 250, 679
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 287, 296, 338
HpyF3I CTNAG 1 cut(s) 427
HpySE526I ACGT 2 cut(s) 234, 335
Hsp92II CATG 1 cut(s) 275
HspAI GCGC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 505
LweI GCATC 1 cut(s) 127
MaeI CTAG 4 cut(s) 215, 405, 452, 611
MaeII ACGT 2 cut(s) 234, 335
MaeIII GTNAC 1 cut(s) 649
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 2 cut(s) 349, 676
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 196
MluCI AATT 2 cut(s) 306, 673
MluNI TGGCCA 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 4 cut(s) 212, 240, 366, 539
Mox20I TGGCCA 1 cut(s) 196
MscI TGGCCA 1 cut(s) 196
MseI TTAA 4 cut(s) 62, 489, 528, 618
Msp20I TGGCCA 1 cut(s) 196
MspI CCGG 4 cut(s) 134, 167, 176, 496
MspR9I CCNGG 5 cut(s) 134, 167, 176, 193, 476
MvaI CCWGG 2 cut(s) 193, 476
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 4 cut(s) 180, 287, 296, 338
NciI CCSGG 3 cut(s) 134, 167, 176
NdeII GATC 1 cut(s) 505
NlaIII CATG 1 cut(s) 275
NlaIV GGNNCC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PfeI GAWTC 6 cut(s) 137, 142, 188, 364, 479, 596
PflFI GACNNNGTC 1 cut(s) 167
PsiI TTATAA 1 cut(s) 691
Psp6I CCWGG 2 cut(s) 191, 474
PspGI CCWGG 2 cut(s) 191, 474
PspN4I GGNNCC 1 cut(s) 657
PspPI GGNCC 2 cut(s) 211, 227
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 5 cut(s) 33, 98, 113, 450, 538
RsaNI GTAC 5 cut(s) 32, 97, 112, 449, 537
SaqAI TTAA 4 cut(s) 62, 489, 528, 618
Sau3AI GATC 1 cut(s) 505
Sau96I GGNCC 2 cut(s) 211, 227
ScaI AGTACT 2 cut(s) 450, 538
ScrFI CCNGG 5 cut(s) 134, 167, 176, 193, 476
SduI GDGCHC 1 cut(s) 252
SetI ASST 8 cut(s) 43, 157, 237, 255, 321, 328, 338, 377
SfaNI GCATC 1 cut(s) 127
Sse9I AATT 2 cut(s) 306, 673
SspMI CTAG 4 cut(s) 215, 405, 452, 611
StyD4I CCNGG 5 cut(s) 132, 165, 174, 191, 474
TaaI ACNGT 1 cut(s) 261
TaiI ACGT 2 cut(s) 237, 338
TaqI TCGA 2 cut(s) 140, 150
TasI AATT 2 cut(s) 306, 673
TatI WGTACW 3 cut(s) 31, 448, 536
TfiI GAWTC 6 cut(s) 137, 142, 188, 364, 479, 596
Tru1I TTAA 4 cut(s) 62, 489, 528, 618
Tru9I TTAA 4 cut(s) 62, 489, 528, 618
Tth111I GACNNNGTC 1 cut(s) 167
VneI GTGCAC 1 cut(s) 248
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 1 cut(s) 673
XbaI TCTAGA 1 cut(s) 610
XspI CTAG 4 cut(s) 215, 405, 452, 611
ZrmI AGTACT 2 cut(s) 450, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.