AT1G22950

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004916
Physical Location & Seq
Reverse (-)
8125023 .. 8127286
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22950.1

Sequence Viewer

Length: 1194 bp
ATGTGCAATCAAACTCCTCTTAGATCTATGGCTCTCGATTCTTCCGGAAAACAACCTGAGCAGCAGCAACAACAACAACCACGAGCCTCCTCTGGAAACGGCGAGGCAAGATTGAAGCTTCGGAGAACCCCTAATGAAGAACATGAGCCTGAGAACTATGAAGATCTACCTTTGGACTATAGTCCTTCTCTGTTCACCTCTCTTGAGCGTTACTTACCTGAGCAGCTTCTCAATTCCACACGAATCGATAAAGCTAGTTTCATGAGAGACCTTCTTCTCCGATATTCCCCTGATACTGAGCGAGTTCGGGTTCTGAGGCATAAAGAATACAGAGACAAAATCATGTCTTCTTACCAACGTCTGCATGGAGAGATCTATACTCTTGACCCTAGTAGTTTCTTTGCGCCATCGTTTCTTGGAGCTTTTAGTCGGAAGTCAGAGCCGAATTTCAGAAGTAGTATGGTTGAATCTTATCCTGGGATTTTCACTTTTGAAATGTTTAAACCACAGTTTTGTGAAATGTTATTAGCAGAGGTTGAACATATGGAGAAATGGGTTTATGATTCAAGATCCACAATAATGAGACCTAATACGATGAACAATTTCGGTGTTGTCCTTGATGATTTTGGCTTTGATAGCATGCTTCAGAAGTTGGTTGATGACTTTATTAGTCCTATAGCTCAAGTTCTGTTCCCGGAAGTCTGTGGAACCTCTTTAGATTCTCACCATGGCTATATTGTTGAATATGGAAAAGATAGGGATGTTGATCTTGGGTTTCATGTGGATGACTCAGAAGTTAGTTTAAATGTCTGCTTGGGTAAACAATTCTCTGGCGGGGAGCTGTATTTTCGAGGGGTGAGATGTGATAAGCATGTGAATTCCGATAGCACTGAAAAGGAAGTTTATGATTACTCTCATGTACCTGGTCACGCCATTCTTCATCGTGGACGTCATCGCCATGGTGCTAGAGCTACAACTTCTGGACACCGAGCCAACTTGATTTTGTGGTGTAGAAGCTCAACTTTTCGAGAGATGAAGAATTATCAGAGAGATTTCTCAGGTTGGTGTGGAGGATGCAAACTTGATAAACAGAGAAGGCAGCGTGATTCGATTAATGCAACTAAAGAGATACTAGCAAGGAAAGCTGCAGAGAAGACACTTGTTGAGCTGGCTTCAAAATCATGTGCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

397

Amino Acids

45.75

Weight (kDa)

6.55

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 114 - 236 1.1e-49 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 952
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 1 cut(s) 834
AclWI GGATC 1 cut(s) 564
AcsI RAATTY 2 cut(s) 445, 877
AcuI CTGAAG 1 cut(s) 629
AcyI GRCGYC 1 cut(s) 949
AfaI GTAC 1 cut(s) 921
AgsI TTSAA 7 cut(s) 115, 467, 494, 539, 567, 743, 1176
AjnI CCWGG 2 cut(s) 475, 922
Alw26I GTCTC 3 cut(s) 261, 327, 577
AlwI GGATC 1 cut(s) 564
Aor13HI TCCGGA 1 cut(s) 44
ApeKI GCWGC 5 cut(s) 61, 64, 223, 1099, 1145
ApoI RAATTY 2 cut(s) 445, 877
AseI ATTAAT 1 cut(s) 1113
Asp700I GAANNNNTTC 1 cut(s) 602
AspLEI GCGC 1 cut(s) 406
AsuC2I CCSGG 1 cut(s) 695
AsuHPI GGTGA 3 cut(s) 187, 716, 868
BauI CACGAG 1 cut(s) 81
BbsI GAAGAC 2 cut(s) 339, 1160
BbvI GCAGC 5 cut(s) 73, 76, 235, 1111, 1132
BccI CCATC 1 cut(s) 415
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 2 cut(s) 477, 924
BcnI CCSGG 1 cut(s) 695
BcoDI GTCTC 3 cut(s) 261, 327, 577
BfaI CTAG 4 cut(s) 255, 390, 966, 1133
BfmI CTRYAG 3 cut(s) 178, 675, 1146
BglII AGATCT 3 cut(s) 23, 163, 372
BisI GCNGC 5 cut(s) 62, 65, 224, 1100, 1146
BlsI GCNGC 5 cut(s) 63, 66, 225, 1101, 1147
Bme1390I CCNGG 3 cut(s) 477, 695, 924
BmiI GGNNCC 1 cut(s) 709
BmrFI CCNGG 3 cut(s) 477, 695, 924
BmsI GCATC 1 cut(s) 1064
BoxI GACNNNNGTC 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 339, 1160
Bpu10I CCTNAGC 2 cut(s) 57, 219
BpuEI CTTGAG 2 cut(s) 224, 666
BpuMI CCSGG 1 cut(s) 695
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 949
BsaI GGTCTC 2 cut(s) 261, 577
BsaJI CCNNGG 3 cut(s) 476, 727, 958
BsaWI WCCGGW 1 cut(s) 44
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 44
BseBI CCWGG 2 cut(s) 477, 924
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 476, 727, 958
BseGI GGATG 3 cut(s) 766, 790, 1079
BseJI GATNNNNATC 1 cut(s) 765
BseMII CTCAG 7 cut(s) 48, 141, 210, 288, 305, 804, 1071
BseRI GAGGAG 2 cut(s) 6, 79
BseXI GCAGC 5 cut(s) 73, 76, 235, 1111, 1132
BshVI ATCGAT 1 cut(s) 246
BsiSI CCGG 2 cut(s) 45, 695
BsmAI GTCTC 3 cut(s) 261, 327, 577
Bso31I GGTCTC 2 cut(s) 261, 577
Bsp13I TCCGGA 1 cut(s) 44
Bsp143I GATC 5 cut(s) 23, 163, 372, 569, 766
Bsp19I CCATGG 2 cut(s) 727, 958
BspACI CCGC 1 cut(s) 834
BspCNI CTCAG 7 cut(s) 49, 142, 211, 289, 306, 803, 1070
BspDI ATCGAT 1 cut(s) 246
BspEI TCCGGA 1 cut(s) 44
BspHI TCATGA 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 709
BspMAI CTGCAG 1 cut(s) 1150
BspPI GGATC 1 cut(s) 564
BspTNI GGTCTC 2 cut(s) 261, 577
BssECI CCNNGG 3 cut(s) 476, 727, 958
BssMI GATC 5 cut(s) 23, 163, 372, 569, 766
BssNI GRCGYC 1 cut(s) 949
BssSI CACGAG 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 727, 958
Bst2BI CACGAG 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 477, 924
Bst4CI ACNGT 1 cut(s) 510
BstACI GRCGYC 1 cut(s) 949
BstC8I GCNNGC 2 cut(s) 641, 1170
BstDEI CTNAG 8 cut(s) 20, 57, 150, 219, 297, 314, 790, 1057
BstDSI CCRYGG 2 cut(s) 727, 958
BstF5I GGATG 3 cut(s) 766, 790, 1079
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 5 cut(s) 26, 166, 375, 572, 769
BstMAI GTCTC 3 cut(s) 261, 327, 577
BstMBI GATC 5 cut(s) 23, 163, 372, 569, 766
BstMWI GCNNNNNNNGC 2 cut(s) 636, 1142
BstNI CCWGG 2 cut(s) 477, 924
BstNSI RCATGY 2 cut(s) 643, 875
BstPAI GACNNNNGTC 1 cut(s) 180
BstSCI CCNGG 3 cut(s) 475, 693, 922
BstSFI CTRYAG 3 cut(s) 178, 675, 1146
BstV1I GCAGC 5 cut(s) 73, 76, 235, 1111, 1132
BstV2I GAAGAC 2 cut(s) 339, 1160
BstX2I RGATCY 4 cut(s) 23, 163, 372, 569
BstYI RGATCY 4 cut(s) 23, 163, 372, 569
Bsu15I ATCGAT 1 cut(s) 246
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 2 cut(s) 727, 958
BtgZI GCGATG 1 cut(s) 938
BtsCI GGATG 3 cut(s) 766, 790, 1079
BtsIMutI CAGTG 1 cut(s) 888
Cac8I GCNNGC 2 cut(s) 641, 1170
CciI TCATGA 1 cut(s) 261
CfoI GCGC 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 246
CsiI ACCWGGT 1 cut(s) 922
Csp6I GTAC 1 cut(s) 920
CviQI GTAC 1 cut(s) 920
DdeI CTNAG 8 cut(s) 20, 57, 150, 219, 297, 314, 790, 1057
DpnI GATC 5 cut(s) 25, 165, 374, 571, 768
DpnII GATC 5 cut(s) 23, 163, 372, 569, 766
DraI TTTAAA 2 cut(s) 502, 804
Eco130I CCWWGG 2 cut(s) 727, 958
Eco31I GGTCTC 2 cut(s) 261, 577
Eco57I CTGAAG 1 cut(s) 629
EcoRI GAATTC 1 cut(s) 877
EcoRII CCWGG 2 cut(s) 475, 922
EcoT14I CCWWGG 2 cut(s) 727, 958
ErhI CCWWGG 2 cut(s) 727, 958
FalI AAGNNNNNCTT 2 cut(s) 1006, 1038
FauI CCCGC 1 cut(s) 827
FauNDI CATATG 1 cut(s) 543
Fnu4HI GCNGC 5 cut(s) 62, 65, 224, 1100, 1146
FokI GGATG 3 cut(s) 773, 797, 1086
Fsp4HI GCNGC 5 cut(s) 62, 65, 224, 1100, 1146
FspBI CTAG 4 cut(s) 255, 390, 966, 1133
GlaI GCGC 1 cut(s) 405
GluI GCNGC 5 cut(s) 62, 65, 224, 1100, 1146
HapII CCGG 2 cut(s) 45, 695
HhaI GCGC 1 cut(s) 406
Hin1I GRCGYC 1 cut(s) 949
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 7 cut(s) 38, 243, 467, 563, 719, 788, 1106
HpaII CCGG 2 cut(s) 45, 695
HphI GGTGA 3 cut(s) 187, 716, 868
Hpy166II GTNNAC 3 cut(s) 195, 821, 947
Hpy188III TCNNGA 9 cut(s) 35, 45, 93, 203, 262, 383, 567, 981, 1028
Hpy8I GTNNAC 3 cut(s) 195, 821, 947
HpyAV CCTTC 3 cut(s) 195, 281, 1089
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4IV ACGT 2 cut(s) 358, 949
HpyCH4V TGCA 5 cut(s) 6, 364, 1077, 1118, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 636, 1142
HpyF3I CTNAG 8 cut(s) 20, 57, 150, 219, 297, 314, 790, 1057
HpySE526I ACGT 2 cut(s) 358, 949
Hsp92I GRCGYC 1 cut(s) 949
HspAI GCGC 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 44
Kzo9I GATC 5 cut(s) 23, 163, 372, 569, 766
LmnI GCTCC 2 cut(s) 419, 838
Lsp1109I GCAGC 5 cut(s) 73, 76, 235, 1111, 1132
LweI GCATC 1 cut(s) 1064
MabI ACCWGGT 1 cut(s) 922
MaeI CTAG 4 cut(s) 255, 390, 966, 1133
MaeII ACGT 2 cut(s) 358, 949
MaeIII GTNAC 2 cut(s) 209, 926
MalI GATC 5 cut(s) 25, 165, 374, 571, 768
MboI GATC 5 cut(s) 23, 163, 372, 569, 766
MboII GAAGA 8 cut(s) 33, 149, 173, 266, 339, 929, 1048, 1165
MflI RGATCY 4 cut(s) 23, 163, 372, 569
MluCI AATT 6 cut(s) 232, 445, 601, 824, 877, 1039
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 1 cut(s) 410
MroI TCCGGA 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 602
MseI TTAA 3 cut(s) 501, 803, 1113
MslI CAYNNNNRTG 3 cut(s) 578, 783, 957
MspI CCGG 2 cut(s) 45, 695
MspR9I CCNGG 3 cut(s) 477, 695, 924
MssI GTTTAAAC 1 cut(s) 502
MvaI CCWGG 2 cut(s) 477, 924
MwoI GCNNNNNNNGC 2 cut(s) 636, 1142
NciI CCSGG 1 cut(s) 695
NcoI CCATGG 2 cut(s) 727, 958
NdeI CATATG 1 cut(s) 543
NdeII GATC 5 cut(s) 23, 163, 372, 569, 766
NlaIV GGNNCC 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 926
NspI RCATGY 2 cut(s) 643, 875
PaeI GCATGC 1 cut(s) 643
PagI TCATGA 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 6 cut(s) 38, 243, 467, 563, 719, 1106
PfoI TCCNGGA 1 cut(s) 693
PkrI GCNGC 5 cut(s) 63, 66, 225, 1101, 1147
PleI GAGTC 1 cut(s) 782
PmeI GTTTAAAC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 782
PshAI GACNNNNGTC 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 1113
Psp6I CCWGG 2 cut(s) 475, 922
PspGI CCWGG 2 cut(s) 475, 922
PspN4I GGNNCC 1 cut(s) 709
PstI CTGCAG 1 cut(s) 1150
PsuI RGATCY 4 cut(s) 23, 163, 372, 569
RsaI GTAC 1 cut(s) 921
RsaNI GTAC 1 cut(s) 920
RseI CAYNNNNRTG 3 cut(s) 578, 783, 957
SaqAI TTAA 3 cut(s) 501, 803, 1113
SatI GCNGC 5 cut(s) 62, 65, 224, 1100, 1146
Sau3AI GATC 5 cut(s) 23, 163, 372, 569, 766
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 3 cut(s) 477, 695, 924
SexAI ACCWGGT 1 cut(s) 922
SfaNI GCATC 1 cut(s) 1064
SfcI CTRYAG 3 cut(s) 178, 675, 1146
SmiMI CAYNNNNRTG 3 cut(s) 578, 783, 957
SmlI CTYRAG 2 cut(s) 203, 681
SmoI CTYRAG 2 cut(s) 203, 681
SphI GCATGC 1 cut(s) 643
Sse9I AATT 6 cut(s) 232, 445, 601, 824, 877, 1039
SsiI CCGC 1 cut(s) 834
SspMI CTAG 4 cut(s) 255, 390, 966, 1133
StyD4I CCNGG 3 cut(s) 475, 693, 922
StyI CCWWGG 2 cut(s) 727, 958
TaaI ACNGT 1 cut(s) 510
TaiI ACGT 2 cut(s) 361, 952
TaqI TCGA 5 cut(s) 36, 246, 850, 1027, 1109
TasI AATT 6 cut(s) 232, 445, 601, 824, 877, 1039
TfiI GAWTC 6 cut(s) 38, 243, 467, 563, 719, 1106
Tru1I TTAA 3 cut(s) 501, 803, 1113
Tru9I TTAA 3 cut(s) 501, 803, 1113
TscAI CASTG 1 cut(s) 895
TseFI GTSAC 1 cut(s) 926
TseI GCWGC 5 cut(s) 61, 64, 223, 1099, 1145
Tsp45I GTSAC 1 cut(s) 926
TspDTI ATGAA 7 cut(s) 150, 174, 250, 611, 767, 929, 1049
TspRI CASTG 1 cut(s) 895
VspI ATTAAT 1 cut(s) 1113
XapI RAATTY 2 cut(s) 445, 877
XceI RCATGY 2 cut(s) 643, 875
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 4 cut(s) 255, 390, 966, 1133
ZraI GACGTC 1 cut(s) 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.