Rorug04G0273700

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004916
Physical Location & Seq
Forward (+)
44619238 .. 44620214
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0273700.1

Sequence Viewer

Length: 483 bp
ATGGGTGTCTTCACTTATGAAACCGAGTTCATCTCCGTCATTCCACCAGCTAGATTGTTCAAGGCTTTTATCCTTGATGCCGACAATCTCATCCCCAAGCTTGCTCCACAAGCAGTCAAGGGTGCTGAAATCATCGAAGGAGATGGTGGTGTTGGAACCATCAAGAAGATCACCTTCGGTGAAGGCAGCCAGTACAGCTCTGTGACACACAAGATCGATGCGATTGACAGTGATAACTTTGTGTACAACTACAGTTTAATTGAAGGGGATGCTATCTCAGACAAGATTGAGAAGATCTCATATGAGACCAAGTTGGTGGCATCTTCCGATGGAGGATCCGTCATCAAGAGCACCAGTAACTACTACACCAAAGGTGATGTGGAGATCAAGGAAGAGCATGTTAAGGCTGGAAAAGAGAAGGCATCCCACCTTTTCAAGCTTGTTGAAGGCTACCTCTTGGCCAATCCTAATGAATACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.47

Weight (kDa)

4.98

Isoelectric Point (pI)

21.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 1.5e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 330, 343
AcoI YGGCCR 1 cut(s) 459
AfaI GTAC 2 cut(s) 194, 245
AgsI TTSAA 4 cut(s) 61, 263, 436, 446
AluBI AGCT 4 cut(s) 50, 100, 198, 439
AluI AGCT 4 cut(s) 50, 100, 198, 439
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 2 cut(s) 330, 343
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 1 cut(s) 186
AsuHPI GGTGA 3 cut(s) 163, 191, 386
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 198
BccI CCATC 3 cut(s) 137, 167, 323
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 1 cut(s) 250
BglII AGATCT 1 cut(s) 294
BisI GCNGC 1 cut(s) 187
BlsI GCNGC 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 157, 337
BmsI GCATC 5 cut(s) 67, 208, 259, 329, 431
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
Bsa29I ATCGAT 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse1I ACTGG 2 cut(s) 190, 354
BseCI ATCGAT 1 cut(s) 216
BseGI GGATG 3 cut(s) 90, 274, 422
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 190, 354
BseXI GCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 461
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 299
BsnI GGCC 1 cut(s) 461
Bso31I GGTCTC 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 353
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 5 cut(s) 168, 213, 294, 335, 384
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 1 cut(s) 290
BspDI ATCGAT 1 cut(s) 216
BspLI GGNNCC 2 cut(s) 157, 337
BspPI GGATC 2 cut(s) 330, 343
BspQI GCTCTTC 1 cut(s) 387
BspTNI GGTCTC 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 2 cut(s) 190, 354
BssMI GATC 5 cut(s) 168, 213, 294, 335, 384
Bst4CI ACNGT 2 cut(s) 230, 254
Bst6I CTCTTC 1 cut(s) 387
BstAUI TGTACA 1 cut(s) 243
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 277
BstF5I GGATG 3 cut(s) 90, 274, 422
BstKTI GATC 5 cut(s) 171, 216, 297, 338, 387
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 5 cut(s) 168, 213, 294, 335, 384
BstMWI GCNNNNNNNGC 2 cut(s) 110, 195
BstNSI RCATGY 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 294, 335
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 2 cut(s) 294, 335
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 1 cut(s) 461
BsuTUI ATCGAT 1 cut(s) 216
BtsCI GGATG 3 cut(s) 90, 274, 422
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 102
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 2 cut(s) 193, 244
CviAII CATG 1 cut(s) 398
CviJI RGCY 9 cut(s) 50, 65, 100, 189, 198, 407, 439, 450, 461
CviKI_1 RGCY 9 cut(s) 50, 65, 100, 189, 198, 407, 439, 450, 461
CviQI GTAC 2 cut(s) 193, 244
DdeI CTNAG 1 cut(s) 277
DpnI GATC 5 cut(s) 170, 215, 296, 337, 386
DpnII GATC 5 cut(s) 168, 213, 294, 335, 384
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 299
FaeI CATG 1 cut(s) 401
FaiI YATR 4 cut(s) 18, 301, 303, 399
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 1 cut(s) 397
FauNDI CATATG 1 cut(s) 301
Fnu4HI GCNGC 1 cut(s) 187
FokI GGATG 3 cut(s) 77, 281, 409
Fsp4HI GCNGC 1 cut(s) 187
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 461
Hin1II CATG 1 cut(s) 401
HindIII AAGCTT 2 cut(s) 98, 437
HphI GGTGA 3 cut(s) 163, 191, 386
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 2 cut(s) 280, 328
Hpy188III TCNNGA 2 cut(s) 163, 346
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 6 cut(s) 131, 176, 184, 257, 412, 440
HpyCH4III ACNGT 2 cut(s) 230, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 195
HpyF3I CTNAG 1 cut(s) 277
Hsp92II CATG 1 cut(s) 401
Kzo9I GATC 5 cut(s) 168, 213, 294, 335, 384
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 4 cut(s) 60, 203, 367, 393
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 5 cut(s) 67, 208, 259, 329, 431
MaeI CTAG 1 cut(s) 51
MaeIII GTNAC 2 cut(s) 202, 356
MalI GATC 5 cut(s) 170, 215, 296, 337, 386
MboI GATC 5 cut(s) 168, 213, 294, 335, 384
MboII GAAGA 4 cut(s) 178, 304, 315, 404
MflI RGATCY 2 cut(s) 294, 335
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 2 cut(s) 326, 464
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 2 cut(s) 257, 402
Msp20I TGGCCA 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 110, 195
NdeI CATATG 1 cut(s) 301
NdeII GATC 5 cut(s) 168, 213, 294, 335, 384
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 157, 337
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 387
PkrI GCNGC 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 2 cut(s) 294, 335
RsaI GTAC 2 cut(s) 194, 245
RsaNI GTAC 2 cut(s) 193, 244
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 257, 402
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 5 cut(s) 168, 213, 294, 335, 384
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 52, 102, 176, 200, 376, 432, 441, 456
SfaNI GCATC 5 cut(s) 67, 208, 259, 329, 431
SfcI CTRYAG 1 cut(s) 250
Sse9I AATT 1 cut(s) 258
SspMI CTAG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 230, 254
TaqI TCGA 2 cut(s) 135, 216
TasI AATT 1 cut(s) 258
TatI WGTACW 2 cut(s) 192, 243
Tru1I TTAA 2 cut(s) 257, 402
Tru9I TTAA 2 cut(s) 257, 402
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 186
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 19, 33
TspGWI ACGGA 2 cut(s) 25, 328
TspRI CASTG 1 cut(s) 235
XceI RCATGY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.