Rorug04G0274000

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004916
Physical Location & Seq
Forward (+)
44631969 .. 44632783
815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0274000.1

Sequence Viewer

Length: 483 bp
ATGGGTGTCTTCACATACGAAACTGAGTTCACCTCTGTCATTCCTCCAGCTAGGTTGTTTAAGGCCTTTGTTCTTGATGCTGACAACTTCATTCCCAAGATTGCTCCACAAGCAGTGAAAAGCGCTGAGATTGTTAAGGGAGATGGCGGTGTTGGAACCATCAAGAAGATCAATCTCGGTGAAGGAAGTGAATACAGCTACGTGAAGCACCAGATTGACGGAATTGACAAAGACAACTTTGTGTACAAGTACAGCATGATCGAAGGAGATGCTATCTCAGACAAAATTGAGAAGATCTCCTACGAGACTAAGTTGGTGGCATCTTCCGATGGAGGCTCCGTTATTAAGAGCACTAGCAACTACCACACCAAGGGAGATGTCGAGATCAAGGAAGAGCATGTTAAGGCTGGCAAAGAAAGGGCAGCTGGTTTGTTCAAGATCATCGAGAGCCACCTTTTGGCCAACCCTGATGCCTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.51

Weight (kDa)

5.59

Isoelectric Point (pI)

16.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 6.3e-26 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 457
AciI CCGC 1 cut(s) 147
AcoI YGGCCR 1 cut(s) 459
AfaI GTAC 2 cut(s) 245, 251
AfeI AGCGCT 1 cut(s) 124
AfiI CCNNNNNNNGG 2 cut(s) 370, 457
AgsI TTSAA 1 cut(s) 436
AluBI AGCT 3 cut(s) 50, 198, 425
AluI AGCT 3 cut(s) 50, 198, 425
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 299
Aor51HI AGCGCT 1 cut(s) 124
AoxI GGCC 2 cut(s) 63, 459
ApeKI GCWGC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 22, 191
BalI TGGCCA 1 cut(s) 461
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 434
BccI CCATC 3 cut(s) 137, 167, 323
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 51, 354
BfoI RGCGCY 1 cut(s) 126
BglII AGATCT 1 cut(s) 294
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 157, 337
BmsI GCATC 4 cut(s) 67, 259, 329, 460
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BpmI CTGGAG 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 457
BseDI CCNNGG 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 370, 457
BseMII CTCAG 3 cut(s) 15, 117, 291
BseXI GCAGC 1 cut(s) 434
BshFI GGCC 2 cut(s) 65, 461
BsiHKAI GWGCWC 1 cut(s) 353
BslI CCNNNNNNNGG 2 cut(s) 370, 457
BsmAI GTCTC 1 cut(s) 299
BsnI GGCC 2 cut(s) 65, 461
Bsp1286I GDGCHC 1 cut(s) 353
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 5 cut(s) 168, 258, 294, 384, 438
BspACI CCGC 1 cut(s) 147
BspANI GGCC 2 cut(s) 65, 461
BspCNI CTCAG 3 cut(s) 16, 118, 290
BspLI GGNNCC 2 cut(s) 157, 337
BspQI GCTCTTC 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 5 cut(s) 168, 258, 294, 384, 438
BssT1I CCWWGG 1 cut(s) 369
Bst6I CTCTTC 1 cut(s) 387
BstAUI TGTACA 1 cut(s) 243
BstBAI YACGTR 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 4 cut(s) 24, 126, 277, 309
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 5 cut(s) 171, 261, 297, 387, 441
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 5 cut(s) 168, 258, 294, 384, 438
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 2 cut(s) 65, 461
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 409
CfoI GCGC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 244, 250
CviAII CATG 2 cut(s) 256, 398
CviJI RGCY 8 cut(s) 50, 65, 198, 336, 407, 425, 450, 461
CviKI_1 RGCY 8 cut(s) 50, 65, 198, 336, 407, 425, 450, 461
CviQI GTAC 2 cut(s) 244, 250
DdeI CTNAG 4 cut(s) 24, 126, 277, 309
DpnI GATC 5 cut(s) 170, 260, 296, 386, 440
DpnII GATC 5 cut(s) 168, 258, 294, 384, 438
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 65
Eco47III AGCGCT 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 2 cut(s) 259, 401
FaiI YATR 3 cut(s) 16, 257, 399
FatI CATG 2 cut(s) 255, 397
Fnu4HI GCNGC 1 cut(s) 423
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 2 cut(s) 51, 354
GlaI GCGC 1 cut(s) 124
GluI GCNGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 2 cut(s) 65, 461
HhaI GCGC 1 cut(s) 125
Hin1II CATG 2 cut(s) 259, 401
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HphI GGTGA 2 cut(s) 22, 191
Hpy166II GTNNAC 2 cut(s) 30, 244
Hpy188I TCNGA 2 cut(s) 280, 328
Hpy188III TCNNGA 5 cut(s) 74, 163, 382, 436, 445
Hpy8I GTNNAC 2 cut(s) 30, 244
HpyAV CCTTC 2 cut(s) 176, 257
HpyCH4IV ACGT 1 cut(s) 201
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 4 cut(s) 24, 126, 277, 309
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 259, 401
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 5 cut(s) 168, 258, 294, 384, 438
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 2 cut(s) 109, 341
LpnPI CCDG 4 cut(s) 60, 224, 393, 411
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 4 cut(s) 67, 259, 329, 460
MaeI CTAG 2 cut(s) 51, 354
MaeII ACGT 1 cut(s) 201
MalI GATC 5 cut(s) 170, 260, 296, 386, 440
MboI GATC 5 cut(s) 168, 258, 294, 384, 438
MboII GAAGA 4 cut(s) 178, 304, 315, 404
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 2 cut(s) 222, 285
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 3 cut(s) 43, 54, 326
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 4 cut(s) 60, 135, 345, 402
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 425
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 5 cut(s) 168, 258, 294, 384, 438
NlaIII CATG 2 cut(s) 259, 401
NlaIV GGNNCC 2 cut(s) 157, 337
NspI RCATGY 1 cut(s) 401
PceI AGGCCT 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 387
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 1 cut(s) 424
Ppu21I YACGTR 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 2 cut(s) 245, 251
RsaNI GTAC 2 cut(s) 244, 250
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 4 cut(s) 60, 135, 345, 402
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 5 cut(s) 168, 258, 294, 384, 438
SduI GDGCHC 1 cut(s) 353
SetI ASST 7 cut(s) 35, 52, 56, 200, 204, 427, 456
SfaNI GCATC 4 cut(s) 67, 259, 329, 460
Sse9I AATT 2 cut(s) 222, 285
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 1 cut(s) 147
SspMI CTAG 2 cut(s) 51, 354
StuI AGGCCT 1 cut(s) 65
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 1 cut(s) 204
TaqI TCGA 3 cut(s) 261, 381, 444
TasI AATT 2 cut(s) 222, 285
TatI WGTACW 2 cut(s) 243, 249
Tru1I TTAA 4 cut(s) 60, 135, 345, 402
Tru9I TTAA 4 cut(s) 60, 135, 345, 402
TscAI CASTG 1 cut(s) 120
TseI GCWGC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 79
TspGWI ACGGA 2 cut(s) 234, 328
TspRI CASTG 1 cut(s) 120
Van91I CCANNNNNTGG 1 cut(s) 457
XceI RCATGY 1 cut(s) 401
XspI CTAG 2 cut(s) 51, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.