MD13G1033500.v1.1

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004916
Physical Location & Seq
Forward (+)
2346768 .. 2350526
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1033500.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGGCTACGGACGGCGCGGCGGACCGACGGAGGCCACCGCCGCAAATGCTGAGCGCAGGCAACAACGGGAACTGCGTGATGAGCTTGTCGAACAGGTTGAGGCTGATGCCGAACAAGGAGCACAAGGCGGAGAGCTACGAGGACCTACAGCATTTGGAGTTCAGTCCCTTGCTTTTCAGCTCGCTGGAGCGCTACTTGCCTCCCACCATGCTCAATGCCTCACGTGACCTCAAGCTCCAGTACATGAGGGACATCCTCCTCCGGTACTCGCCCGAGAGCGAACGCACTCGTGTTCAACGACATCGAGAATACAGGCAGAAGATCATTGCACACTATCAGCCTCTACAAAGGGAGTTATACACGCTGCAAGCTGCAAAGTTCTTTGTCCCCTCATTTCTCAGAGCAATCAGTGAGAACACAGAGGCTAGCTTTAGAAATATACTGTCTGAACCCTCTCCTGGAGTTTATACCTTTGAAATGCTTCAGCCGGATTTCTGTGAATTGTTATTAACTGAGGTGGAAAATTTTGAAGGGTGGGTCCATGAGACAAATTTCAGAATCATGCGACCAAATACAATGAATAGATACGGTTGTGTTCTTGATGACTTTGGTCTTGAAACCATGCTTGACAAGTTGATGGAGGACTTTATACGTCCTTTGTCTAAATTTTTCTTCCCAGAAGTTGGCGGATCCACACTGGATACTCATCACGGTTTTGTTGTTGAATATGGAAGCGATAGGGATGTAGAGCTTGGTTTTCATGTGGATGATTCAGAAGTCACCTTGAATGTTTGCTTGGGAAAGCAATTTTCTGGTGGAGATTTGTTCTTTCGAGGTGTTCGATGTGATAAACATGTTAATTCAGAGACACAATCTGAGGAAATCTTTGATTATACTCATGTTCCGGGACGTGCCATTCTTCATCATGGTCGCCATCGGCATGGTGCTAGAGCCACAACATCGGGGCATCGAGTCAACTTACTATTATGGTGCAGAAGTTCGGTGTTTAGAGAGCTGAAGAAATATCAGAAAGATTTTTCCAAATGGTGTGGAGAGTGCAAACGTGAAAAAAAAGAGAGGCAGCGTCAATCAATTGCTGCAACCAAACTGGAAATACTCAAGAGAGATGGACAAACCGCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

382

Amino Acids

44.33

Weight (kDa)

7.67

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 108 - 230 4.9e-55 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 683
AccII CGCG 1 cut(s) 17
AciI CCGC 7 cut(s) 17, 20, 38, 41, 128, 687, 1137
AclWI GGATC 2 cut(s) 684, 697
AcsI RAATTY 3 cut(s) 523, 550, 665
AcuI CTGAAG 2 cut(s) 467, 1037
AcvI CACGTG 1 cut(s) 224
AfaI GTAC 2 cut(s) 242, 266
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 2 cut(s) 458, 683
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 6 cut(s) 296, 476, 530, 617, 725, 787
AjiI CACGTC 1 cut(s) 911
AjnI CCWGG 1 cut(s) 457
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AluBI AGCT 8 cut(s) 84, 135, 180, 235, 371, 429, 751, 1015
AluI AGCT 8 cut(s) 84, 135, 180, 235, 371, 429, 751, 1015
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 539, 860
AlwI GGATC 2 cut(s) 684, 697
Ama87I CYCGRG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 4 cut(s) 364, 371, 1081, 1097
ApoI RAATTY 3 cut(s) 523, 550, 665
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 3 cut(s) 17, 56, 192
AspS9I GGNCC 3 cut(s) 22, 142, 538
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 1 cut(s) 772
AsuNHI GCTAGC 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 3 cut(s) 22, 142, 538
BaeI ACNNNNGTAYC 2 cut(s) 577, 610
BamHI GGATCC 1 cut(s) 689
BauI CACGAG 1 cut(s) 288
BbrPI CACGTG 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 351, 358, 1084, 1093
BccI CCATC 3 cut(s) 631, 942, 1121
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 459
BciVI GTATCC 1 cut(s) 694
BcnI CCSGG 1 cut(s) 906
BcoDI GTCTC 2 cut(s) 539, 860
BfaI CTAG 2 cut(s) 426, 948
BfmI CTRYAG 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 193
BfuI GTATCC 1 cut(s) 694
BisI GCNGC 6 cut(s) 18, 41, 365, 372, 1082, 1098
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 6 cut(s) 19, 42, 366, 373, 1083, 1099
Bme1390I CCNGG 2 cut(s) 459, 906
Bme18I GGWCC 3 cut(s) 22, 142, 538
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 911
BmgT120I GGNCC 3 cut(s) 22, 142, 538
BmiI GGNNCC 2 cut(s) 539, 691
BmrFI CCNGG 2 cut(s) 459, 906
BmsI GCATC 2 cut(s) 96, 976
BmtI GCTAGC 1 cut(s) 429
BoxI GACNNNNGTC 1 cut(s) 609
BpmI CTGGAG 3 cut(s) 206, 221, 480
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 215, 1103
BpuMI CCSGG 1 cut(s) 906
BsaAI YACGTR 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 705
BsaWI WCCGGW 1 cut(s) 261
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 683
Bse1I ACTGG 3 cut(s) 238, 702, 1113
Bse3DI GCAATG 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 1 cut(s) 459
BseGI GGATG 3 cut(s) 252, 748, 772
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 2 cut(s) 458, 683
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 4 cut(s) 41, 412, 504, 867
BseNI ACTGG 3 cut(s) 238, 702, 1113
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 4 cut(s) 351, 358, 1084, 1093
BsgI GTGCAG 1 cut(s) 1012
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 3 cut(s) 262, 488, 905
BslFI GGGAC 4 cut(s) 150, 263, 371, 921
BslI CCNNNNNNNGG 2 cut(s) 458, 683
BsmAI GTCTC 2 cut(s) 539, 860
BsmFI GGGAC 4 cut(s) 150, 263, 371, 921
BsnI GGCC 1 cut(s) 34
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 2 cut(s) 321, 689
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 7 cut(s) 17, 20, 38, 41, 128, 687, 1137
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 4 cut(s) 42, 411, 505, 868
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 539, 691
BspOI GCTAGC 1 cut(s) 429
BspPI GGATC 2 cut(s) 684, 697
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 3 cut(s) 238, 702, 1113
BssMI GATC 2 cut(s) 321, 689
BssSI CACGAG 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 3 cut(s) 444, 590, 713
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 4 cut(s) 58, 182, 369, 427
BstDEI CTNAG 4 cut(s) 50, 398, 513, 876
BstF5I GGATG 3 cut(s) 252, 748, 772
BstFNI CGCG 1 cut(s) 17
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 3 cut(s) 17, 56, 192
BstKTI GATC 2 cut(s) 324, 692
BstMAI GTCTC 2 cut(s) 539, 860
BstMBI GATC 2 cut(s) 321, 689
BstMWI GCNNNNNNNGC 4 cut(s) 40, 46, 81, 196
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 1 cut(s) 857
BstPAI GACNNNNGTC 1 cut(s) 609
BstSCI CCNGG 2 cut(s) 457, 904
BstSFI CTRYAG 1 cut(s) 146
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 351, 358, 1084, 1093
BstX2I RGATCY 1 cut(s) 689
BstXI CCANNNNNNTGG 1 cut(s) 941
BstYI RGATCY 1 cut(s) 689
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 1 cut(s) 34
BtrI CACGTC 1 cut(s) 911
BtsCI GGATG 3 cut(s) 252, 748, 772
BtsIMutI CAGTG 2 cut(s) 415, 695
Cac8I GCNNGC 4 cut(s) 58, 182, 369, 427
CfoI GCGC 3 cut(s) 17, 56, 192
Cfr13I GGNCC 3 cut(s) 22, 142, 538
CpoI CGGWCCG 1 cut(s) 22
CseI GACGC 1 cut(s) 1073
Csp6I GTAC 2 cut(s) 241, 265
CspCI CAANNNNNGTGG 2 cut(s) 1030, 1065
CspI CGGWCCG 1 cut(s) 22
CviQI GTAC 2 cut(s) 241, 265
DdeI CTNAG 4 cut(s) 50, 398, 513, 876
DpnI GATC 2 cut(s) 323, 691
DpnII GATC 2 cut(s) 321, 689
EciI GGCGGA 3 cut(s) 35, 143, 702
Eco47I GGWCC 3 cut(s) 22, 142, 538
Eco47III AGCGCT 1 cut(s) 191
Eco57I CTGAAG 2 cut(s) 467, 1037
Eco72I CACGTG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 457
FaqI GGGAC 4 cut(s) 150, 263, 371, 921
Fnu4HI GCNGC 6 cut(s) 18, 41, 365, 372, 1082, 1098
FokI GGATG 3 cut(s) 239, 755, 779
Fsp4HI GCNGC 6 cut(s) 18, 41, 365, 372, 1082, 1098
FspBI CTAG 2 cut(s) 426, 948
GlaI GCGC 3 cut(s) 16, 55, 191
GluI GCNGC 6 cut(s) 18, 41, 365, 372, 1082, 1098
GsuI CTGGAG 3 cut(s) 206, 221, 480
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 1 cut(s) 34
HapII CCGG 3 cut(s) 262, 488, 905
HgaI GACGC 1 cut(s) 1073
HhaI GCGC 3 cut(s) 17, 56, 192
Hin6I GCGC 3 cut(s) 15, 54, 190
HinP1I GCGC 3 cut(s) 15, 54, 190
HincII GTYRAC 1 cut(s) 976
HindII GTYRAC 1 cut(s) 976
HinfI GANTC 3 cut(s) 558, 770, 972
HpaII CCGG 3 cut(s) 262, 488, 905
HphI GGTGA 1 cut(s) 772
Hpy166II GTNNAC 1 cut(s) 976
Hpy188I TCNGA 7 cut(s) 401, 448, 557, 775, 865, 877, 1029
Hpy188III TCNNGA 5 cut(s) 305, 599, 614, 1120, 1143
Hpy8I GTNNAC 1 cut(s) 976
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 524
HpyCH4III ACNGT 3 cut(s) 444, 590, 713
HpyCH4IV ACGT 4 cut(s) 223, 652, 910, 1063
HpyCH4V TGCA 6 cut(s) 329, 367, 374, 993, 1059, 1100
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 46, 81, 196
HpyF3I CTNAG 4 cut(s) 50, 398, 513, 876
HpySE526I ACGT 4 cut(s) 223, 652, 910, 1063
HspAI GCGC 3 cut(s) 15, 54, 190
Kzo9I GATC 2 cut(s) 321, 689
LmnI GCTCC 3 cut(s) 118, 187, 240
Lsp1109I GCAGC 4 cut(s) 351, 358, 1084, 1093
LweI GCATC 2 cut(s) 96, 976
MaeI CTAG 2 cut(s) 426, 948
MaeII ACGT 4 cut(s) 223, 652, 910, 1063
MaeIII GTNAC 2 cut(s) 224, 778
MalI GATC 2 cut(s) 323, 691
MboI GATC 2 cut(s) 321, 689
MboII GAAGA 4 cut(s) 331, 664, 911, 1030
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 1 cut(s) 689
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 7 cut(s) 500, 523, 550, 665, 806, 859, 1092
MlyI GAGTC 1 cut(s) 981
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 2 cut(s) 509, 858
MslI CAYNNNNRTG 2 cut(s) 765, 939
MspI CCGG 3 cut(s) 262, 488, 905
MspR9I CCNGG 2 cut(s) 459, 906
MunI CAATTG 1 cut(s) 1092
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 4 cut(s) 40, 46, 81, 196
NciI CCSGG 1 cut(s) 906
NdeII GATC 2 cut(s) 321, 689
NheI GCTAGC 1 cut(s) 425
NlaIV GGNNCC 2 cut(s) 539, 691
NmuCI GTSAC 2 cut(s) 224, 778
NspI RCATGY 1 cut(s) 857
PciI ACATGT 1 cut(s) 853
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 295, 838
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 2 cut(s) 558, 770
PflMI CCANNNNNTGG 1 cut(s) 683
PfoI TCCNGGA 2 cut(s) 457, 904
PkrI GCNGC 6 cut(s) 19, 42, 366, 373, 1083, 1099
PleI GAGTC 1 cut(s) 980
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
PpsI GAGTC 1 cut(s) 980
Ppu21I YACGTR 1 cut(s) 224
PpuMI RGGWCCY 1 cut(s) 142
PscI ACATGT 1 cut(s) 853
PshAI GACNNNNGTC 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 457
PspCI CACGTG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 539, 691
PspPI GGNCC 3 cut(s) 22, 142, 538
PspPPI RGGWCCY 1 cut(s) 142
PsuI RGATCY 1 cut(s) 689
RsaI GTAC 2 cut(s) 242, 266
RsaNI GTAC 2 cut(s) 241, 265
RseI CAYNNNNRTG 2 cut(s) 765, 939
Rsr2I CGGWCCG 1 cut(s) 22
RsrII CGGWCCG 1 cut(s) 22
SaqAI TTAA 2 cut(s) 509, 858
SatI GCNGC 6 cut(s) 18, 41, 365, 372, 1082, 1098
Sau3AI GATC 2 cut(s) 321, 689
Sau96I GGNCC 3 cut(s) 22, 142, 538
SchI GAGTC 1 cut(s) 981
ScrFI CCNGG 2 cut(s) 459, 906
SduI GDGCHC 1 cut(s) 123
SfaNI GCATC 2 cut(s) 96, 976
SfcI CTRYAG 1 cut(s) 146
SinI GGWCC 3 cut(s) 22, 142, 538
SmiMI CAYNNNNRTG 2 cut(s) 765, 939
SmlI CTYRAG 2 cut(s) 230, 1118
SmoI CTYRAG 2 cut(s) 230, 1118
Sse9I AATT 7 cut(s) 500, 523, 550, 665, 806, 859, 1092
SsiI CCGC 7 cut(s) 17, 20, 38, 41, 128, 687, 1137
SspMI CTAG 2 cut(s) 426, 948
StyD4I CCNGG 2 cut(s) 457, 904
TaaI ACNGT 3 cut(s) 444, 590, 713
TaiI ACGT 4 cut(s) 226, 655, 913, 1066
TaqI TCGA 5 cut(s) 89, 304, 832, 841, 970
TaqII GACCGA 1 cut(s) 39
TasI AATT 7 cut(s) 500, 523, 550, 665, 806, 859, 1092
TatI WGTACW 1 cut(s) 240
TauI GCSGC 2 cut(s) 20, 43
TfiI GAWTC 2 cut(s) 558, 770
Tru1I TTAA 2 cut(s) 509, 858
Tru9I TTAA 2 cut(s) 509, 858
TscAI CASTG 2 cut(s) 415, 702
TseFI GTSAC 2 cut(s) 224, 778
TseI GCWGC 4 cut(s) 364, 371, 1081, 1097
Tsp45I GTSAC 2 cut(s) 224, 778
TspDTI ATGAA 3 cut(s) 593, 749, 911
TspGWI ACGGA 2 cut(s) 23, 43
TspRI CASTG 2 cut(s) 415, 702
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 3 cut(s) 22, 142, 538
XapI RAATTY 3 cut(s) 523, 550, 665
XceI RCATGY 1 cut(s) 857
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 2 cut(s) 426, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.