Rorug04G0273600

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004916
Physical Location & Seq
Reverse (-)
44611501 .. 44611863
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0273600.1

Sequence Viewer

Length: 363 bp
ATGGGCGTGTTCACTTACGAATCCGAGTTCGCCTCTGTCATTGCACCACCAAGACTGTTCAAGGCCTTTGTTCTTGATGCTGACAACCTCATCCCCAAGATTGCCCCACAGGCTGTGAAGAGTGCTGAAATTGTTGAAGGAGATGGAGGTGTTGGAACCATCAAGAAGATTCATCTTGGTGAAGGAAGCGAATACAGCTACGTGACGCATCAGATTAATGGACTGGACAAAGACAACTTTGTTTACAAGTACAGCATCATTGAAGGTGATGCTATTGGAGACAAAGTTGAGAAAATCTCGGACGAGATTAAACTGGTGGCATCTCCAAGTGGAGGTTCTATCATCAAGAGAAAAGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

12.92

Weight (kDa)

5.35

Isoelectric Point (pI)

20.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 116 2e-15 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 3 cut(s) 61, 137, 263
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 273
AoxI GGCC 1 cut(s) 63
AseI ATTAAT 1 cut(s) 216
AsuHPI GGTGA 2 cut(s) 191, 278
BccI CCATC 2 cut(s) 137, 167
BcoDI GTCTC 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 5 cut(s) 67, 217, 259, 264, 329
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BsaAI YACGTR 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse1I ACTGG 2 cut(s) 228, 318
Bse3DI GCAATG 1 cut(s) 39
BseGI GGATG 1 cut(s) 90
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 39
BseNI ACTGG 2 cut(s) 228, 318
BshFI GGCC 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 273
BsnI GGCC 1 cut(s) 65
BspANI GGCC 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 2 cut(s) 228, 318
Bst4CI ACNGT 1 cut(s) 57
Bst6I CTCTTC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 202
BstF5I GGATG 1 cut(s) 90
BstMAI GTCTC 1 cut(s) 273
BstMWI GCNNNNNNNGC 2 cut(s) 110, 195
BsuRI GGCC 1 cut(s) 65
BtsCI GGATG 1 cut(s) 90
CseI GACGC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 250
CviJI RGCY 3 cut(s) 65, 113, 198
CviKI_1 RGCY 3 cut(s) 65, 113, 198
CviQI GTAC 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco147I AGGCCT 1 cut(s) 65
FaiI YATR 1 cut(s) 361
FokI GGATG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 65
HgaI GACGC 1 cut(s) 214
HinfI GANTC 2 cut(s) 20, 169
HphI GGTGA 2 cut(s) 191, 278
Hpy166II GTNNAC 2 cut(s) 12, 244
Hpy188I TCNGA 3 cut(s) 25, 213, 301
Hpy188III TCNNGA 3 cut(s) 74, 163, 346
Hpy8I GTNNAC 2 cut(s) 12, 244
HpyAV CCTTC 3 cut(s) 131, 176, 257
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 195
HpySE526I ACGT 1 cut(s) 201
LpnPI CCDG 3 cut(s) 95, 209, 299
LweI GCATC 5 cut(s) 67, 217, 259, 264, 329
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 2 cut(s) 130, 178
MluCI AATT 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 4 cut(s) 43, 98, 140, 326
MseI TTAA 2 cut(s) 216, 309
MslI CAYNNNNRTG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 110, 195
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 202
PceI AGGCCT 1 cut(s) 65
PfeI GAWTC 2 cut(s) 20, 169
Ppu21I YACGTR 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 157
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 2 cut(s) 216, 309
SetI ASST 6 cut(s) 90, 151, 200, 204, 268, 337
SfaNI GCATC 5 cut(s) 67, 217, 259, 264, 329
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 1 cut(s) 129
SseBI AGGCCT 1 cut(s) 65
StuI AGGCCT 1 cut(s) 65
TaaI ACNGT 1 cut(s) 57
TaiI ACGT 1 cut(s) 204
TasI AATT 1 cut(s) 129
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 2 cut(s) 20, 169
Tru1I TTAA 2 cut(s) 216, 309
Tru9I TTAA 2 cut(s) 216, 309
TseFI GTSAC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 161
VspI ATTAAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.