MD16G1035400.v1.1

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004916
Physical Location & Seq
Forward (+)
2536215 .. 2539708
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1035400.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGCTACGGACGGCGCGGCGGACCGACGGAGGCCACCGCCGCAAACGCTGAGCGCAGGCAACAACGGAAACTGCGTGATGAGCTTGTCGAACAGACTGAGACTGATGCCGAACAAGGATCACAAGGCGGAGAGCTACGAGGACCTGGAGCAGTTGGAGTTCAGTCCCTTGCTCTTCAGCTCGCTGGAGCGCTACTTGCCACCCACCATGCTCAATGCCTCACGTGACCTCAAGCTCCAGTACATGAGGGACATTCTCCTCCGGTACTCACCCGAGAGCGAACGCACTCGTGTTCAACGACATCGAGAATACAGGCAGAAGATCATCACACACTATCAGCCTCTACAAAGGGAGTTGTACACTCTGCAAGCTGAAAAGTTCTTTGTCCCCTCATTTCTCAGAGCAATCAGTGAGAACACAGAGGATAGCTTTAGAAGTATACTGTCTGAACCCTCTCCTGGAGTTTATACATTTGAAATGCTTCAGCCGGATTTCTGTGACTTGTTAGTAACTGAGGTGGAAAATTTTCAAGGGTGGGTCCATGAGACAAATTTCAGAGTCATGCGACCAAATACAATGAATAAATACGGTTGTGTTCTTGATGACTTTGGCCTGGAAACCATGCTTGACAAGTTGATGGAGGACTTTATACGTCCTATGTCCAAAGTTTTCTTCCCGGAAGTTGGTGGATCCACACTGGATACTCATCACGGTTTTGTTGTTGAATATGGAATCGATAGGGATGTAGAGCTTGGTTTTCATGTGGATGATTCAGAAGTCACCTTGAATGTTTGCTTGGGAAAGCAATTTTCAGGTGGAGATTTGTTCTTTCGAGGTGTTCGATGTGATAAACATGTTAATTCAGAGACACAATCTGAGGAAATCTTTGATTATCCTCATGTCCCGGGACATGCCATTCTTCATCATGGTCGCCATCGACATGGTGCTAGAGCCACGACATCAGGGCGTCGAGTCAACTTATTATTATGGTGCAGAAGTTCGGTCTTTAGGGAGCTGAAGAAATATCAGAAAGACTTTTCCAACTGGTGTGGCGAGTGCAAACGTGAGAAGAAAGAAAGGCAGCGCCAATCAATTGCCGCAACCAAACTGGAAATACTTAAGATAAATGGACAAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

44.16

Weight (kDa)

6.71

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 108 - 230 1.9e-55 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 439
AccII CGCG 1 cut(s) 17
AciI CCGC 6 cut(s) 17, 20, 38, 41, 128, 1098
AclWI GGATC 3 cut(s) 126, 684, 697
AcsI RAATTY 2 cut(s) 523, 550
AcuI CTGAAG 3 cut(s) 160, 467, 1037
AcvI CACGTG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 967
AfaI GTAC 3 cut(s) 242, 266, 359
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 2 cut(s) 458, 683
AflII CTTAAG 1 cut(s) 1118
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 5 cut(s) 296, 476, 530, 725, 787
AjnI CCWGG 3 cut(s) 144, 457, 612
AjuI GAANNNNNNNTTGG 4 cut(s) 656, 688, 779, 811
AluBI AGCT 8 cut(s) 84, 135, 180, 235, 371, 429, 751, 1015
AluI AGCT 8 cut(s) 84, 135, 180, 235, 371, 429, 751, 1015
Alw26I GTCTC 3 cut(s) 94, 539, 860
AlwI GGATC 3 cut(s) 126, 684, 697
Ama87I CYCGRG 2 cut(s) 272, 904
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 2 cut(s) 32, 610
ApeKI GCWGC 1 cut(s) 1081
ApoI RAATTY 2 cut(s) 523, 550
Asp700I GAANNNNTTC 2 cut(s) 480, 525
AspLEI GCGC 4 cut(s) 17, 56, 192, 1086
AspS9I GGNCC 3 cut(s) 22, 142, 538
AsuC2I CCSGG 3 cut(s) 677, 905, 906
AsuHPI GGTGA 2 cut(s) 261, 772
AvaI CYCGRG 2 cut(s) 272, 904
AvaII GGWCC 3 cut(s) 22, 142, 538
BamHI GGATCC 1 cut(s) 689
BauI CACGAG 1 cut(s) 288
BbrPI CACGTG 1 cut(s) 224
BbvI GCAGC 1 cut(s) 1093
BccI CCATC 2 cut(s) 631, 942
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 3 cut(s) 146, 459, 614
BciVI GTATCC 1 cut(s) 694
BcnI CCSGG 3 cut(s) 677, 905, 906
BcoDI GTCTC 3 cut(s) 94, 539, 860
BfaI CTAG 1 cut(s) 948
BfoI RGCGCY 2 cut(s) 193, 1087
BfrI CTTAAG 1 cut(s) 1118
BfuI GTATCC 1 cut(s) 694
BisI GCNGC 4 cut(s) 18, 41, 1082, 1098
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 4 cut(s) 19, 42, 1083, 1099
Bme1390I CCNGG 6 cut(s) 146, 459, 614, 677, 905, 906
Bme18I GGWCC 3 cut(s) 22, 142, 538
BmeT110I CYCGRG 2 cut(s) 272, 904
BmgT120I GGNCC 3 cut(s) 22, 142, 538
BmiI GGNNCC 2 cut(s) 539, 691
BmrFI CCNGG 6 cut(s) 146, 459, 614, 677, 905, 906
BmsI GCATC 1 cut(s) 96
BpmI CTGGAG 4 cut(s) 167, 206, 221, 480
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 3 cut(s) 677, 905, 906
Bsa29I ATCGAT 1 cut(s) 735
BsaAI YACGTR 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 967
BsaJI CCNNGG 1 cut(s) 904
BsaWI WCCGGW 1 cut(s) 261
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 683
Bse1I ACTGG 4 cut(s) 238, 702, 1049, 1113
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 3 cut(s) 146, 459, 614
BseCI ATCGAT 1 cut(s) 735
BseDI CCNNGG 1 cut(s) 904
BseGI GGATG 2 cut(s) 748, 772
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 2 cut(s) 458, 683
BseMII CTCAG 5 cut(s) 41, 89, 412, 504, 867
BseNI ACTGG 4 cut(s) 238, 702, 1049, 1113
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 1093
BsgI GTGCAG 1 cut(s) 1012
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 34, 612
BshVI ATCGAT 1 cut(s) 735
BsiHKCI CYCGRG 2 cut(s) 272, 904
BsiSI CCGG 4 cut(s) 262, 488, 677, 905
BslFI GGGAC 5 cut(s) 150, 263, 371, 887, 921
BslI CCNNNNNNNGG 2 cut(s) 458, 683
BsmAI GTCTC 3 cut(s) 94, 539, 860
BsmFI GGGAC 5 cut(s) 150, 263, 371, 887, 921
BsnI GGCC 2 cut(s) 34, 612
BsoBI CYCGRG 2 cut(s) 272, 904
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 3 cut(s) 118, 321, 689
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 6 cut(s) 17, 20, 38, 41, 128, 1098
BspANI GGCC 2 cut(s) 34, 612
BspCNI CTCAG 5 cut(s) 42, 90, 411, 505, 868
BspDI ATCGAT 1 cut(s) 735
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 539, 691
BspPI GGATC 3 cut(s) 126, 684, 697
BspQI GCTCTTC 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 1118
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 4 cut(s) 238, 702, 1049, 1113
BssECI CCNNGG 1 cut(s) 904
BssMI GATC 3 cut(s) 118, 321, 689
BssNAI GTATAC 1 cut(s) 440
BssNI GRCGYC 1 cut(s) 967
BssSI CACGAG 1 cut(s) 288
Bst1107I GTATAC 1 cut(s) 440
Bst2BI CACGAG 1 cut(s) 288
Bst2UI CCWGG 3 cut(s) 146, 459, 614
Bst4CI ACNGT 3 cut(s) 444, 590, 713
Bst6I CTCTTC 1 cut(s) 179
BstACI GRCGYC 1 cut(s) 967
BstAFI CTTAAG 1 cut(s) 1118
BstAUI TGTACA 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 58, 182, 369
BstDEI CTNAG 5 cut(s) 50, 98, 398, 513, 876
BstF5I GGATG 2 cut(s) 748, 772
BstFNI CGCG 1 cut(s) 17
BstH2I RGCGCY 2 cut(s) 193, 1087
BstHHI GCGC 4 cut(s) 17, 56, 192, 1086
BstKTI GATC 3 cut(s) 121, 324, 692
BstMAI GTCTC 3 cut(s) 94, 539, 860
BstMBI GATC 3 cut(s) 118, 321, 689
BstMWI GCNNNNNNNGC 4 cut(s) 40, 46, 81, 196
BstNI CCWGG 3 cut(s) 146, 459, 614
BstNSI RCATGY 2 cut(s) 857, 914
BstSCI CCNGG 6 cut(s) 144, 457, 612, 675, 903, 904
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 1093
BstX2I RGATCY 1 cut(s) 689
BstXI CCANNNNNNTGG 1 cut(s) 941
BstYI RGATCY 1 cut(s) 689
BstZ17I GTATAC 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 735
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 2 cut(s) 34, 612
BsuTUI ATCGAT 1 cut(s) 735
BtsCI GGATG 2 cut(s) 748, 772
BtsIMutI CAGTG 2 cut(s) 415, 695
Cac8I GCNNGC 3 cut(s) 58, 182, 369
CfoI GCGC 4 cut(s) 17, 56, 192, 1086
Cfr13I GGNCC 3 cut(s) 22, 142, 538
Cfr9I CCCGGG 1 cut(s) 904
ClaI ATCGAT 1 cut(s) 735
CpoI CGGWCCG 1 cut(s) 22
CseI GACGC 1 cut(s) 956
Csp6I GTAC 3 cut(s) 241, 265, 358
CspCI CAANNNNNGTGG 2 cut(s) 1030, 1065
CspI CGGWCCG 1 cut(s) 22
CviQI GTAC 3 cut(s) 241, 265, 358
DdeI CTNAG 5 cut(s) 50, 98, 398, 513, 876
DpnI GATC 3 cut(s) 120, 323, 691
DpnII GATC 3 cut(s) 118, 321, 689
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
EciI GGCGGA 2 cut(s) 35, 143
Eco47I GGWCC 3 cut(s) 22, 142, 538
Eco47III AGCGCT 1 cut(s) 191
Eco57I CTGAAG 3 cut(s) 160, 467, 1037
Eco72I CACGTG 1 cut(s) 224
Eco88I CYCGRG 2 cut(s) 272, 904
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 3 cut(s) 144, 457, 612
FaqI GGGAC 5 cut(s) 150, 263, 371, 887, 921
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 4 cut(s) 18, 41, 1082, 1098
FokI GGATG 2 cut(s) 755, 779
Fsp4HI GCNGC 4 cut(s) 18, 41, 1082, 1098
FspBI CTAG 1 cut(s) 948
GlaI GCGC 4 cut(s) 16, 55, 191, 1085
GluI GCNGC 4 cut(s) 18, 41, 1082, 1098
GsuI CTGGAG 4 cut(s) 167, 206, 221, 480
HaeII RGCGCY 2 cut(s) 193, 1087
HaeIII GGCC 2 cut(s) 34, 612
HapII CCGG 4 cut(s) 262, 488, 677, 905
HgaI GACGC 1 cut(s) 956
HhaI GCGC 4 cut(s) 17, 56, 192, 1086
Hin1I GRCGYC 1 cut(s) 967
Hin6I GCGC 4 cut(s) 15, 54, 190, 1084
HinP1I GCGC 4 cut(s) 15, 54, 190, 1084
HincII GTYRAC 1 cut(s) 976
HindII GTYRAC 1 cut(s) 976
HinfI GANTC 4 cut(s) 558, 732, 770, 972
HpaII CCGG 4 cut(s) 262, 488, 677, 905
HphI GGTGA 2 cut(s) 261, 772
Hpy166II GTNNAC 3 cut(s) 360, 440, 976
Hpy188I TCNGA 7 cut(s) 401, 448, 557, 775, 865, 877, 1029
Hpy188III TCNNGA 2 cut(s) 305, 599
Hpy8I GTNNAC 3 cut(s) 360, 440, 976
Hpy99I CGWCG 2 cut(s) 30, 972
HpyCH4III ACNGT 3 cut(s) 444, 590, 713
HpyCH4IV ACGT 3 cut(s) 223, 652, 1063
HpyCH4V TGCA 3 cut(s) 367, 993, 1059
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 46, 81, 196
HpyF3I CTNAG 5 cut(s) 50, 98, 398, 513, 876
HpySE526I ACGT 3 cut(s) 223, 652, 1063
Hsp92I GRCGYC 1 cut(s) 967
HspAI GCGC 4 cut(s) 15, 54, 190, 1084
Kzo9I GATC 3 cut(s) 118, 321, 689
LguI GCTCTTC 1 cut(s) 179
LmnI GCTCC 4 cut(s) 148, 187, 240, 1012
Lsp1109I GCAGC 1 cut(s) 1093
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 948
MaeII ACGT 3 cut(s) 223, 652, 1063
MaeIII GTNAC 4 cut(s) 224, 497, 508, 778
MalI GATC 3 cut(s) 120, 323, 691
MboI GATC 3 cut(s) 118, 321, 689
MboII GAAGA 6 cut(s) 166, 331, 664, 911, 1030, 1081
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 1 cut(s) 689
MluCI AATT 5 cut(s) 523, 550, 806, 859, 1092
MlyI GAGTC 2 cut(s) 567, 981
MmeI TCCRAC 2 cut(s) 135, 1065
MroXI GAANNNNTTC 2 cut(s) 480, 525
MseI TTAA 2 cut(s) 858, 1119
MslI CAYNNNNRTG 2 cut(s) 765, 939
MspCI CTTAAG 1 cut(s) 1118
MspI CCGG 4 cut(s) 262, 488, 677, 905
MspR9I CCNGG 6 cut(s) 146, 459, 614, 677, 905, 906
MunI CAATTG 1 cut(s) 1092
MvaI CCWGG 3 cut(s) 146, 459, 614
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 4 cut(s) 40, 46, 81, 196
NciI CCSGG 3 cut(s) 677, 905, 906
NdeII GATC 3 cut(s) 118, 321, 689
NlaIV GGNNCC 2 cut(s) 539, 691
NmuCI GTSAC 3 cut(s) 224, 497, 778
NspI RCATGY 2 cut(s) 857, 914
PciI ACATGT 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 295, 838
PdmI GAANNNNTTC 2 cut(s) 480, 525
PfeI GAWTC 2 cut(s) 732, 770
PfoI TCCNGGA 2 cut(s) 457, 675
PkrI GCNGC 4 cut(s) 19, 42, 1083, 1099
PleI GAGTC 2 cut(s) 566, 980
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
PpsI GAGTC 2 cut(s) 566, 980
Ppu21I YACGTR 1 cut(s) 224
PpuMI RGGWCCY 1 cut(s) 142
PscI ACATGT 1 cut(s) 853
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 3 cut(s) 144, 457, 612
PspCI CACGTG 1 cut(s) 224
PspGI CCWGG 3 cut(s) 144, 457, 612
PspN4I GGNNCC 2 cut(s) 539, 691
PspPI GGNCC 3 cut(s) 22, 142, 538
PspPPI RGGWCCY 1 cut(s) 142
PsuI RGATCY 1 cut(s) 689
RsaI GTAC 3 cut(s) 242, 266, 359
RsaNI GTAC 3 cut(s) 241, 265, 358
RseI CAYNNNNRTG 2 cut(s) 765, 939
Rsr2I CGGWCCG 1 cut(s) 22
RsrII CGGWCCG 1 cut(s) 22
SapI GCTCTTC 1 cut(s) 179
SaqAI TTAA 2 cut(s) 858, 1119
SatI GCNGC 4 cut(s) 18, 41, 1082, 1098
Sau3AI GATC 3 cut(s) 118, 321, 689
Sau96I GGNCC 3 cut(s) 22, 142, 538
SchI GAGTC 2 cut(s) 567, 981
ScrFI CCNGG 6 cut(s) 146, 459, 614, 677, 905, 906
SfaNI GCATC 1 cut(s) 96
SinI GGWCC 3 cut(s) 22, 142, 538
SmaI CCCGGG 1 cut(s) 906
SmiMI CAYNNNNRTG 2 cut(s) 765, 939
SmlI CTYRAG 2 cut(s) 230, 1118
SmoI CTYRAG 2 cut(s) 230, 1118
Sse9I AATT 5 cut(s) 523, 550, 806, 859, 1092
SsiI CCGC 6 cut(s) 17, 20, 38, 41, 128, 1098
SspMI CTAG 1 cut(s) 948
StyD4I CCNGG 6 cut(s) 144, 457, 612, 675, 903, 904
TaaI ACNGT 3 cut(s) 444, 590, 713
TaiI ACGT 3 cut(s) 226, 655, 1066
TaqI TCGA 7 cut(s) 89, 304, 735, 832, 841, 937, 970
TaqII GACCGA 2 cut(s) 39, 991
TasI AATT 5 cut(s) 523, 550, 806, 859, 1092
TatI WGTACW 2 cut(s) 240, 357
TauI GCSGC 3 cut(s) 20, 43, 1100
TfiI GAWTC 2 cut(s) 732, 770
Tru1I TTAA 2 cut(s) 858, 1119
Tru9I TTAA 2 cut(s) 858, 1119
TscAI CASTG 2 cut(s) 415, 702
TseFI GTSAC 3 cut(s) 224, 497, 778
TseI GCWGC 1 cut(s) 1081
Tsp45I GTSAC 3 cut(s) 224, 497, 778
TspDTI ATGAA 3 cut(s) 593, 749, 911
TspGWI ACGGA 3 cut(s) 23, 43, 81
TspMI CCCGGG 1 cut(s) 904
TspRI CASTG 2 cut(s) 415, 702
Vha464I CTTAAG 1 cut(s) 1118
VpaK11BI GGWCC 3 cut(s) 22, 142, 538
XapI RAATTY 2 cut(s) 523, 550
XceI RCATGY 2 cut(s) 857, 914
XmaI CCCGGG 1 cut(s) 904
XmiI GTMKAC 1 cut(s) 439
XmnI GAANNNNTTC 2 cut(s) 480, 525
XspI CTAG 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.