Rorug04G0273800

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004916
Physical Location & Seq
Forward (+)
44621465 .. 44622447
983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0273800.1

Sequence Viewer

Length: 480 bp
ATGGGTGTCTTCACATACGAATCTGAGTTTAACTCTGTCATCCCACCACCTAAGTTGTTCAAGGCCTTTGTCCTTGATGCCGACAACCTCATCCCCAAGATTGCCCCACAAGCTGTGAAGAGTGCCGAAATTGTTGAGGGAGATGGAGGTGTTGGAACCATCAAGAAGATCCACCTTGGTGAAGGAAGTGAGTATAGCTATGTCAAGCACAAGATTGACGGAATTGACAAAGACAACTTTGTGTACAACTACAGCATAATCGAAGGAGACGCTATCGGAGACAAGATTGAGAAAATCTCCTATGAGATTATGTTGGTGGCATCTGAGGGAGGTTCCATCATCAAGAGCACCAGTCACTACCACACCAAGGGTGAGGTCGAGATCAAGGAGGAGCATGTCAAGGCTGGAAAAGAAAGAGCCGCTGGTCTGTTCAAGCTCATTGAAAACCACCTTGTGGCCCATCCTGAGGAATACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.54

Weight (kDa)

5.36

Isoelectric Point (pI)

26.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 153 2.2e-24 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 454
AciI CCGC 1 cut(s) 420
AclWI GGATC 1 cut(s) 163
AdeI CACNNNGTG 1 cut(s) 454
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 3 cut(s) 367, 454, 466
AgsI TTSAA 3 cut(s) 61, 433, 443
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 3 cut(s) 113, 198, 436
AluI AGCT 3 cut(s) 113, 198, 436
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 2 cut(s) 261, 273
AlwI GGATC 1 cut(s) 163
AoxI GGCC 2 cut(s) 63, 456
AspS9I GGNCC 1 cut(s) 457
AsuHPI GGTGA 2 cut(s) 191, 383
AxyI CCTNAGG 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 350
BccI CCATC 4 cut(s) 137, 167, 344, 468
BcoDI GTCTC 2 cut(s) 261, 273
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 2 cut(s) 157, 334
BmsI GCATC 2 cut(s) 67, 329
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BsaJI CCNNGG 2 cut(s) 175, 366
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 454, 466
Bse1I ACTGG 1 cut(s) 351
Bse21I CCTNAGG 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 175, 366
BseGI GGATG 3 cut(s) 39, 90, 460
BseLI CCNNNNNNNGG 3 cut(s) 367, 454, 466
BseMII CTCAG 3 cut(s) 15, 315, 456
BseNI ACTGG 1 cut(s) 351
BseRI GAGGAG 1 cut(s) 404
BshFI GGCC 2 cut(s) 65, 458
BsiHKAI GWGCWC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 367, 454, 466
BsmAI GTCTC 2 cut(s) 261, 273
BsmBI CGTCTC 1 cut(s) 261
BsnI GGCC 2 cut(s) 65, 458
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 2 cut(s) 168, 381
BspACI CCGC 1 cut(s) 420
BspANI GGCC 2 cut(s) 65, 458
BspCNI CTCAG 3 cut(s) 16, 316, 457
BspLI GGNNCC 2 cut(s) 157, 334
BspPI GGATC 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 351
BssECI CCNNGG 2 cut(s) 175, 366
BssMI GATC 2 cut(s) 168, 381
BssT1I CCWWGG 2 cut(s) 175, 366
Bst6I CTCTTC 1 cut(s) 113
BstAUI TGTACA 1 cut(s) 243
BstDEI CTNAG 4 cut(s) 24, 51, 324, 465
BstF5I GGATG 3 cut(s) 39, 90, 460
BstKTI GATC 2 cut(s) 171, 384
BstMAI GTCTC 2 cut(s) 261, 273
BstMBI GATC 2 cut(s) 168, 381
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 398
BstSFI CTRYAG 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 465
BsuRI GGCC 2 cut(s) 65, 458
BtsCI GGATG 3 cut(s) 39, 90, 460
Cfr13I GGNCC 1 cut(s) 457
CseI GACGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 244
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 1 cut(s) 395
CviJI RGCY 7 cut(s) 65, 113, 198, 404, 419, 436, 458
CviKI_1 RGCY 7 cut(s) 65, 113, 198, 404, 419, 436, 458
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 4 cut(s) 24, 51, 324, 465
DpnI GATC 2 cut(s) 170, 383
DpnII GATC 2 cut(s) 168, 381
DraIII CACNNNGTG 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 175, 366
Eco147I AGGCCT 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 465
EcoT14I CCWWGG 2 cut(s) 175, 366
ErhI CCWWGG 2 cut(s) 175, 366
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 1 cut(s) 398
FaiI YATR 7 cut(s) 16, 195, 201, 257, 303, 311, 396
FatI CATG 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 3 cut(s) 26, 77, 447
Fsp4HI GCNGC 1 cut(s) 420
GluI GCNGC 1 cut(s) 420
HaeIII GGCC 2 cut(s) 65, 458
HgaI GACGC 1 cut(s) 278
Hin1II CATG 1 cut(s) 398
HinfI GANTC 1 cut(s) 20
HphI GGTGA 2 cut(s) 191, 383
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 25, 278, 325
Hpy188III TCNNGA 4 cut(s) 163, 343, 379, 464
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 176, 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 4 cut(s) 24, 51, 324, 465
Hsp92II CATG 1 cut(s) 398
Kzo9I GATC 2 cut(s) 168, 381
LmnI GCTCC 1 cut(s) 391
LpnPI CCDG 3 cut(s) 364, 390, 408
LweI GCATC 2 cut(s) 67, 329
MaeIII GTNAC 1 cut(s) 353
MalI GATC 2 cut(s) 170, 383
MboI GATC 2 cut(s) 168, 381
MboII GAAGA 2 cut(s) 130, 178
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 2 cut(s) 129, 222
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 8 cut(s) 98, 130, 140, 319, 323, 367, 382, 460
MseI TTAA 1 cut(s) 30
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 422
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 2 cut(s) 168, 381
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 2 cut(s) 157, 334
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 177
PceI AGGCCT 1 cut(s) 65
PfeI GAWTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 454
PkrI GCNGC 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 157, 334
PspPI GGNCC 1 cut(s) 457
PsuI RGATCY 1 cut(s) 168
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 2 cut(s) 168, 381
Sau96I GGNCC 1 cut(s) 457
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 2 cut(s) 67, 329
SfcI CTRYAG 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 2 cut(s) 129, 222
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 1 cut(s) 420
StuI AGGCCT 1 cut(s) 65
StyI CCWWGG 2 cut(s) 175, 366
TaqI TCGA 2 cut(s) 261, 378
TasI AATT 2 cut(s) 129, 222
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TseFI GTSAC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 234
Van91I CCANNNNNTGG 1 cut(s) 454
XceI RCATGY 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.