Rorug04G0273900

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004916
Physical Location & Seq
Forward (+)
44623843 .. 44624721
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0273900.1

Sequence Viewer

Length: 483 bp
ATGGGTGTGTTCACTTATGAAACCGAGTTCACCTCTGTCATCCCCCCTGCTAGGTTGTTCAAGTCCTTTGTTCTTGATGCCGACAACCTCATCCCCAAGATTGCTCCCCAAGCAGTTAAAAGTGCCGAGATTCTTGAAGGAGATGGAGGAGTTGGAACCATCAAGAAGATCAACCTCGGGGAAGGAAGTGAATATAGCTATGTGAAGCATCAGATTGATGGAATTGACAAGGAAAACTTTGTGTACAGTTATAGTATGATTGAGGGAGATGCTATCTCAGACAAAATTGAGAAGATCTCCTATGAGACTAAGTTGGTTGCATCTTCTGATGGAGGTTCTGTCATCAAGAGCACCAGCAACTACCACACCAAGGGCGACGTCGAGATCAAGGAAGAGCATGTTAAGGCAGGAAAAGAAAGGGCTGCTGGTTTATTCAAGATTATTGAGAGCCACCTTTTGGCCAATCCTGATGCCTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.48

Weight (kDa)

5.11

Isoelectric Point (pI)

19.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 5.3e-27 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 381
AccB7I CCANNNNNTGG 1 cut(s) 457
AcoI YGGCCR 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 3 cut(s) 51, 370, 457
AgsI TTSAA 3 cut(s) 61, 137, 436
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 176
BalI TGGCCA 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 409
BccI CCATC 4 cut(s) 137, 167, 212, 323
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 1 cut(s) 51
BglII AGATCT 1 cut(s) 294
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
BmeT110I CYCGRG 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 5 cut(s) 67, 217, 259, 329, 460
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 175, 369
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 370, 457
BseDI CCNNGG 2 cut(s) 175, 369
BseGI GGATG 2 cut(s) 39, 90
BseLI CCNNNNNNNGG 3 cut(s) 51, 370, 457
BseMII CTCAG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 409
BshFI GGCC 1 cut(s) 461
BsiHKAI GWGCWC 1 cut(s) 353
BsiHKCI CYCGRG 1 cut(s) 176
BslI CCNNNNNNNGG 3 cut(s) 51, 370, 457
BsmAI GTCTC 1 cut(s) 299
BsnI GGCC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 353
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 3 cut(s) 168, 294, 384
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 1 cut(s) 290
BspLI GGNNCC 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 243
BssECI CCNNGG 2 cut(s) 175, 369
BssMI GATC 3 cut(s) 168, 294, 384
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 387
BstACI GRCGYC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 243
BstDEI CTNAG 2 cut(s) 277, 309
BstF5I GGATG 2 cut(s) 39, 90
BstKTI GATC 3 cut(s) 171, 297, 387
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 3 cut(s) 168, 294, 384
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 1 cut(s) 461
BtsCI GGATG 2 cut(s) 39, 90
Csp6I GTAC 1 cut(s) 244
CviAII CATG 1 cut(s) 398
CviJI RGCY 4 cut(s) 198, 422, 450, 461
CviKI_1 RGCY 4 cut(s) 198, 422, 450, 461
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 2 cut(s) 277, 309
DpnI GATC 3 cut(s) 170, 296, 386
DpnII GATC 3 cut(s) 168, 294, 384
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 1 cut(s) 401
FaiI YATR 7 cut(s) 18, 195, 201, 252, 257, 303, 399
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FatI CATG 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 2 cut(s) 26, 77
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 461
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 1 cut(s) 401
HinfI GANTC 1 cut(s) 130
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 3 cut(s) 12, 30, 244
Hpy188I TCNGA 3 cut(s) 213, 280, 328
Hpy188III TCNNGA 7 cut(s) 74, 134, 163, 346, 382, 436, 467
Hpy8I GTNNAC 3 cut(s) 12, 30, 244
Hpy99I CGWCG 2 cut(s) 380, 383
HpyAV CCTTC 2 cut(s) 131, 176
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 277, 309
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 1 cut(s) 401
Kzo9I GATC 3 cut(s) 168, 294, 384
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 4 cut(s) 60, 367, 393, 411
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 5 cut(s) 67, 217, 259, 329, 460
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 378
MalI GATC 3 cut(s) 170, 296, 386
MboI GATC 3 cut(s) 168, 294, 384
MboII GAAGA 4 cut(s) 178, 304, 315, 404
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 2 cut(s) 222, 285
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 6 cut(s) 43, 98, 140, 185, 256, 326
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 2 cut(s) 117, 402
Msp20I TGGCCA 1 cut(s) 461
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 3 cut(s) 168, 294, 384
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 1 cut(s) 157
NmeAIII GCCGAG 1 cut(s) 151
NspI RCATGY 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 130
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 117, 402
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 3 cut(s) 168, 294, 384
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 35, 56, 90, 177, 200, 337, 381, 456
SfaNI GCATC 5 cut(s) 67, 217, 259, 329, 460
Sse9I AATT 2 cut(s) 222, 285
SspMI CTAG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 1 cut(s) 381
TaqI TCGA 1 cut(s) 381
TasI AATT 2 cut(s) 222, 285
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 2 cut(s) 117, 402
Tru9I TTAA 2 cut(s) 117, 402
TseI GCWGC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 33
Van91I CCANNNNNTGG 1 cut(s) 457
XceI RCATGY 1 cut(s) 401
XspI CTAG 1 cut(s) 51
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.