FvH4_4g26460

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004916
Physical Location & Seq
Forward (+)
27859572 .. 27863960
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26460.t3

Sequence Viewer

Length: 1125 bp
ATGGACGGCTCAGTGGACCGAAGTAATCCACCGCCGCAAACGGCGTGCACGGCCAACGGAAACGGCGTCGTTTCGAAGACGAGGCTGAGACTGAAGCCGGAGAAGGAGCACAAGTCGGATAGCTACAACGACCTGCAGCAATTGGATTTCAGTCCTCTGCTCTTCAGCTCGCTCGAGCATTACTTGCCGGCGAACATGCTCAATGTGCCGCGTGAGCTCAAGCTCCGGTACATGAGGGACATTCTGCTCCGGTACACCTCCGAGGGCGAACGCAGTCGTATTCAGCGGCATAGAGAATACAGAGACAGGATCACATCAAGCTATCAGTATCTGCACGAGGAGCTATACACTATGCAAGCTGAACAATTCTTTGTCCCGTCATTTCTCAATGTCATCAATGAGGGCACAGAGGGTACTTTTAGAAGTATACTCAACGAACCCTCTCCTGGAATCTTCACATTTGAAATGCTTCAGCCGCGTTTCTGCGAATTGTTAGTATCCGAGGTTGAGAATTTTGAAAGGTGGGTCCATGAGACAAGATTCAGAATCATGCGACCAAACACAATGAACAAATTTGGTGCTGTTCTTGATGACTTTGGCTTTGAAACTATGCTTGACAAGTTGATGGAGGAATTTATACGCCCTATATCTAAAGTTCTCTTTCCAGAAGTTGGTGGAGCCACACTGGATTCACATCATGGTTTTGTTGTTGAATATGGAATGGATAGGGACGTTGAGCTTGGTTTTCATGTCGATGACTCGGAAGTCACCTTGAATGTTTGCTTGGGTAAGCAGTTTATTGGTGGGGAGTTGTATTTTCGAGGTGTTCGATGTAATAAGCATGTGAATTCAGCGACACTCTCAGAGGAAGTCTTTGATTATCCTCATGTTCCGGGACATGCAGTTCTTCATCGTGGTCGCCACCGACATGGTGCTAGAGCTACAATATCTGGGCGTCGAACCAACTTACTACTATGGTGCAGAAGTTCGGTCTTTAGAGAGCTGAGAAAATATCAAAAAGATTGTTCTAGCTGGTGCGGAGACTGCCATCGGGAGAAGAAAGAAAGACAGCGCCAATCAGTTGCTGCCACCAAACTGGAATTACTTAACAATGATGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

375

Amino Acids

43.53

Weight (kDa)

6.74

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 104 - 226 2.5e-54 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 1 cut(s) 427
AccII CGCG 2 cut(s) 211, 478
AciI CCGC 6 cut(s) 32, 35, 209, 286, 476, 1038
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 4 cut(s) 511, 572, 632, 847
AcuI CTGAAG 3 cut(s) 113, 148, 455
AcyI GRCGYC 2 cut(s) 66, 955
AfaI GTAC 3 cut(s) 230, 254, 415
AfiI CCNNNNNNNGG 2 cut(s) 446, 671
AgsI TTSAA 5 cut(s) 464, 518, 605, 713, 775
AjnI CCWGG 1 cut(s) 445
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AloI GAACNNNNNNTCC 2 cut(s) 888, 920
Alw21I GWGCWC 3 cut(s) 50, 111, 219
Alw26I GTCTC 4 cut(s) 82, 297, 527, 1035
Alw44I GTGCAC 1 cut(s) 46
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 2 cut(s) 331, 1085
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 1 cut(s) 51
ApaLI GTGCAC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 136, 1085
ApoI RAATTY 4 cut(s) 511, 572, 632, 847
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 1074
AspS9I GGNCC 2 cut(s) 16, 526
AsuC2I CCSGG 1 cut(s) 894
AsuHPI GGTGA 1 cut(s) 760
AsuII TTCGAA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 2 cut(s) 16, 526
BaeGI GKGCMC 2 cut(s) 50, 407
BanII GRGCYC 1 cut(s) 219
BauI CACGAG 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 3 cut(s) 50, 111, 219
BbvI GCAGC 2 cut(s) 148, 1072
BccI CCATC 3 cut(s) 619, 1056, 1109
BceAI ACGGC 4 cut(s) 22, 57, 66, 79
BciT130I CCWGG 1 cut(s) 447
BciVI GTATCC 2 cut(s) 508, 1112
BcnI CCSGG 1 cut(s) 894
BcoDI GTCTC 4 cut(s) 82, 297, 527, 1035
BfaI CTAG 3 cut(s) 936, 1029, 1123
BfmI CTRYAG 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 1075
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 2 cut(s) 508, 1112
BisI GCNGC 6 cut(s) 35, 137, 209, 287, 476, 1086
BlsI GCNGC 6 cut(s) 36, 138, 210, 288, 477, 1087
Bme1390I CCNGG 2 cut(s) 447, 894
Bme18I GGWCC 2 cut(s) 16, 526
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 16, 526
BmiI GGNNCC 2 cut(s) 527, 679
BmrFI CCNGG 2 cut(s) 447, 894
BpiI GAAGAC 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 894
BsaBI GATNNNNATC 1 cut(s) 693
BsaHI GRCGYC 2 cut(s) 66, 955
BsaJI CCNNGG 2 cut(s) 261, 501
BsaWI WCCGGW 2 cut(s) 225, 249
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 671
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 690, 1101
Bse8I GATNNNNATC 1 cut(s) 693
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 261, 501
BseJI GATNNNNATC 1 cut(s) 693
BseLI CCNNNNNNNGG 2 cut(s) 446, 671
BseMII CTCAG 4 cut(s) 24, 77, 876, 995
BseNI ACTGG 2 cut(s) 690, 1101
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 2 cut(s) 50, 407
BseXI GCAGC 2 cut(s) 148, 1072
BsgI GTGCAG 2 cut(s) 317, 1000
Bsh1236I CGCG 2 cut(s) 211, 478
BshFI GGCC 1 cut(s) 53
BsiHKAI GWGCWC 3 cut(s) 50, 111, 219
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 5 cut(s) 98, 188, 226, 250, 893
BslFI GGGAC 4 cut(s) 251, 359, 743, 909
BslI CCNNNNNNNGG 2 cut(s) 446, 671
BsmAI GTCTC 4 cut(s) 82, 297, 527, 1035
BsmFI GGGAC 4 cut(s) 251, 359, 743, 909
BsnI GGCC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 4 cut(s) 50, 111, 219, 407
Bsp143I GATC 1 cut(s) 309
BspACI CCGC 6 cut(s) 32, 35, 209, 286, 476, 1038
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 4 cut(s) 23, 78, 875, 996
BspFNI CGCG 2 cut(s) 211, 478
BspLI GGNNCC 2 cut(s) 527, 679
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 1 cut(s) 317
BspQI GCTCTTC 1 cut(s) 167
BspT104I TTCGAA 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 2 cut(s) 690, 1101
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 2 cut(s) 261, 501
BssMI GATC 1 cut(s) 309
BssNAI GTATAC 1 cut(s) 428
BssNI GRCGYC 2 cut(s) 66, 955
BssSI CACGAG 1 cut(s) 335
Bst1107I GTATAC 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 447
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 2 cut(s) 66, 955
BstAPI GCANNNNNTGC 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 46, 170, 189, 357
BstDEI CTNAG 4 cut(s) 10, 86, 862, 1004
BstFNI CGCG 2 cut(s) 211, 478
BstH2I RGCGCY 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 1074
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 4 cut(s) 82, 297, 527, 1035
BstMBI GATC 1 cut(s) 309
BstMWI GCNNNNNNNGC 7 cut(s) 50, 184, 205, 214, 340, 475, 1044
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 3 cut(s) 199, 845, 902
BstSCI CCNGG 2 cut(s) 445, 892
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 2 cut(s) 50, 407
BstUI CGCG 2 cut(s) 211, 478
BstV1I GCAGC 2 cut(s) 148, 1072
BstV2I GAAGAC 1 cut(s) 83
BstXI CCANNNNNNTGG 2 cut(s) 929, 1096
BstZ17I GTATAC 1 cut(s) 428
BsuI GTATCC 2 cut(s) 508, 1112
BsuRI GGCC 1 cut(s) 53
BtsIMutI CAGTG 2 cut(s) 18, 683
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 46, 170, 189, 357
CaiI CAGNNNCTG 2 cut(s) 331, 1085
CfoI GCGC 1 cut(s) 1074
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 16, 526
CseI GACGC 2 cut(s) 55, 944
Csp6I GTAC 3 cut(s) 229, 253, 414
CviQI GTAC 3 cut(s) 229, 253, 414
DdeI CTNAG 4 cut(s) 10, 86, 862, 1004
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 219
Eco47I GGWCC 2 cut(s) 16, 526
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 113, 148, 455
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 219
FaqI GGGAC 4 cut(s) 251, 359, 743, 909
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 6 cut(s) 35, 137, 209, 287, 476, 1086
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 6 cut(s) 35, 137, 209, 287, 476, 1086
FspBI CTAG 3 cut(s) 936, 1029, 1123
GlaI GCGC 1 cut(s) 1073
GluI GCNGC 6 cut(s) 35, 137, 209, 287, 476, 1086
HaeII RGCGCY 1 cut(s) 1075
HaeIII GGCC 1 cut(s) 53
HapII CCGG 5 cut(s) 98, 188, 226, 250, 893
HgaI GACGC 2 cut(s) 55, 944
HhaI GCGC 1 cut(s) 1074
Hin1I GRCGYC 2 cut(s) 66, 955
Hin6I GCGC 1 cut(s) 1072
HinP1I GCGC 1 cut(s) 1072
HinfI GANTC 5 cut(s) 450, 540, 546, 689, 758
HpaII CCGG 5 cut(s) 98, 188, 226, 250, 893
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 4 cut(s) 16, 48, 255, 428
Hpy188I TCNGA 6 cut(s) 118, 262, 502, 545, 763, 865
Hpy188III TCNNGA 3 cut(s) 587, 665, 1052
Hpy8I GTNNAC 4 cut(s) 16, 48, 255, 428
Hpy99I CGWCG 2 cut(s) 71, 960
HpyAV CCTTC 1 cut(s) 97
HpyCH4IV ACGT 1 cut(s) 732
HpyCH4V TGCA 6 cut(s) 48, 136, 334, 355, 902, 981
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 184, 205, 214, 340, 475, 1044
HpyF3I CTNAG 4 cut(s) 10, 86, 862, 1004
HpySE526I ACGT 1 cut(s) 732
Hsp92I GRCGYC 2 cut(s) 66, 955
HspAI GCGC 1 cut(s) 1072
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Kzo9I GATC 1 cut(s) 309
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 5 cut(s) 106, 228, 252, 340, 677
Lsp1109I GCAGC 2 cut(s) 148, 1072
MaeI CTAG 3 cut(s) 936, 1029, 1123
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 1 cut(s) 766
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 5 cut(s) 88, 154, 445, 899, 1069
MfeI CAATTG 1 cut(s) 140
MhlI GDGCHC 4 cut(s) 50, 111, 219, 407
MluCI AATT 8 cut(s) 140, 365, 488, 511, 572, 632, 847, 1100
MlyI GAGTC 1 cut(s) 752
MmeI TCCRAC 1 cut(s) 96
MroNI GCCGGC 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 1 cut(s) 1107
MslI CAYNNNNRTG 2 cut(s) 753, 927
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 5 cut(s) 98, 188, 226, 250, 893
MspR9I CCNGG 2 cut(s) 447, 894
MunI CAATTG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 2 cut(s) 211, 478
MwoI GCNNNNNNNGC 7 cut(s) 50, 184, 205, 214, 340, 475, 1044
NaeI GCCGGC 1 cut(s) 189
NciI CCSGG 1 cut(s) 894
NdeII GATC 1 cut(s) 309
NgoMIV GCCGGC 1 cut(s) 187
NlaIV GGNNCC 2 cut(s) 527, 679
NmuCI GTSAC 1 cut(s) 766
NspI RCATGY 3 cut(s) 199, 845, 902
NspV TTCGAA 1 cut(s) 74
PaeR7I CTCGAG 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 167
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 826
PdiI GCCGGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 4 cut(s) 450, 540, 546, 689
PflMI CCANNNNNTGG 1 cut(s) 671
PfoI TCCNGGA 2 cut(s) 445, 892
PkrI GCNGC 6 cut(s) 36, 138, 210, 288, 477, 1087
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 527, 679
PspPI GGNCC 2 cut(s) 16, 526
PspXI VCTCGAGB 1 cut(s) 173
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 2 cut(s) 331, 1085
RsaI GTAC 3 cut(s) 230, 254, 415
RsaNI GTAC 3 cut(s) 229, 253, 414
RseI CAYNNNNRTG 2 cut(s) 753, 927
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 1 cut(s) 1107
SatI GCNGC 6 cut(s) 35, 137, 209, 287, 476, 1086
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 2 cut(s) 16, 526
SchI GAGTC 1 cut(s) 752
ScrFI CCNGG 2 cut(s) 447, 894
SduI GDGCHC 4 cut(s) 50, 111, 219, 407
SfcI CTRYAG 1 cut(s) 134
Sfr274I CTCGAG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 74
SinI GGWCC 2 cut(s) 16, 526
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 753, 927
SmlI CTYRAG 2 cut(s) 173, 218
SmoI CTYRAG 2 cut(s) 173, 218
Sse9I AATT 8 cut(s) 140, 365, 488, 511, 572, 632, 847, 1100
SsiI CCGC 6 cut(s) 32, 35, 209, 286, 476, 1038
SspMI CTAG 3 cut(s) 936, 1029, 1123
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 445, 892
TaiI ACGT 1 cut(s) 735
TaqI TCGA 6 cut(s) 74, 174, 753, 820, 829, 958
TaqII GACCGA 2 cut(s) 33, 979
TasI AATT 8 cut(s) 140, 365, 488, 511, 572, 632, 847, 1100
TauI GCSGC 4 cut(s) 37, 211, 289, 478
TfiI GAWTC 4 cut(s) 450, 540, 546, 689
Tru1I TTAA 1 cut(s) 1107
Tru9I TTAA 1 cut(s) 1107
TscAI CASTG 2 cut(s) 18, 690
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 2 cut(s) 136, 1085
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 3 cut(s) 581, 737, 899
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 2 cut(s) 18, 690
Van91I CCANNNNNTGG 1 cut(s) 671
VneI GTGCAC 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 16, 526
XapI RAATTY 4 cut(s) 511, 572, 632, 847
XceI RCATGY 3 cut(s) 199, 845, 902
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 3 cut(s) 936, 1029, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.